Ptdss1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptdss1
Entrez Gene ID	19210
Full Name	phosphatidylserine synthase 1
Synonyms	AU044268,AW539008,PSS-1,mKIAA0024
General protein information	Preferred Names phosphatidylserine synthase 1 Names phosphatidylserine synthase 1 PtdSer Synthase-1 ptdSer synthase 1 serine-exchange enzyme I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 B3\|13 34.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008959.3	NP_032985.2	phosphatidylserine synthase 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4825	NP_001262118.1
Caenorhabditis elegans (roundworm)	pssy-1	NP_509673.1
Danio rerio (zebrafish)	ptdss1a	NP_957250.1
Xenopus tropicalis (tropical clawed frog)	ptdss1	NP_001120508.1
Homo sapiens (human)	PTDSS1	NP_055569.1
Bos taurus (cattle)	PTDSS1	NP_001039505.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012146	XP_320381.3
Caenorhabditis elegans (roundworm)	pssy-2	NP_499786.2
	PTDSS1	XP_001091545.1
Rattus norvegicus (Norway rat)	Ptdss1	NP_001012113.1
Pan troglodytes (chimpanzee)	PTDSS1	XP_519868.2
Canis lupus familiaris (dog)	PTDSS1	XP_854779.3
Mus musculus (house mouse)	Ptdss1	NP_032985.2
Gallus gallus (chicken)	PTDSS1	NP_001026676.1
Danio rerio (zebrafish)	ptdss1b	XP_687872.5

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells.
Tasseva G, Cole L, Vance JE
The Journal of biological chemistry286(2)1061-73(2011 Jan)

Defining the importance of phosphatidylserine synthase-1 (PSS1): unexpected viability of PSS1-deficient mice.
Arikketh D, Nelson R, Vance JE
The Journal of biological chemistry283(19)12888-97(2008 May)

Identification of transcripts with enriched expression in the developing and adult pancreas.
Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD
Genome biology9(6)R99(2008)

Synthesis of phosphatidylserine by base exchange in Triton-insoluble floating fractions from rat cerebellum.
Buratta S, Felicetti M, Mozzi R
Journal of neurochemistry103(3)942-51(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Site-directed mutagenesis of binding sites within the Pss1 promoter demonstrated that Sp and N-Myc synergistically activate Pss1 expression in astrocytes
Title: N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells.

elimination of either PSS1 or PSS2, but not both, is compatible with mouse viability
Title: Defining the importance of phosphatidylserine synthase-1 (PSS1): unexpected viability of PSS1-deficient mice.

SBEE, synthesizing PS in the binding area for PKC, participates inn signal transduction. The capability of SBEE to utilize not only serine but also ethanolamine, as free exchanging base, suggests a mechanism for modulating in loco PS concentration.
Title: Synthesis of phosphatidylserine by base exchange in Triton-insoluble floating fractions from rat cerebellum.

The following Ptdss1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptdss1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03188	NM_008959.3 Latest version!	Mus musculus phosphatidylserine synthase 1 (Ptdss1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptdss1 ( NM_008959.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032985.2 Mus musculus phosphatidylserine synthase 1 (Ptdss1), mRNA.

Price & Availability↑

CloneID	OMu03188
Clone ID Related Accession (Same CDS sequence)	NM_008959.3
Accession Version	NM_008959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	phosphatidylserine synthase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK085857.1 and AK054101.1. On Oct 21, 2009 this sequence version replaced NM_008959.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK085857.1, SRR1660821.85144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCT GCGTGGGGAG CAGGACTCTG AGCAAGGATG ATGTGAACTA CAGGATGCAT 
TTCCGCATGA TCAACGAGCA GCAAGTGGAG GACATCACCA TAGACTTCTT CTACCGGCCG 
CACACCATCA CCCTGCTCAG CTTCACCATC ATCAGCCTCA TGTACTTCGC CTTCACCAGG 
GATGACTCTG TTCCGGAAGA CAACATCTGG AGAGGCATCC TGTCTGTTAT CTTCTTCTTT 
CTCATCATCA GCGTGTTAGC ATTCCCCAAT GGTCCATTTA CTCGACCTCA TCCAGCCTTA 
TGGCGAATGG TTTTTGGTCT CAGTGTGTTG TATTTCCTCT TCTTGGTATT CCTCCTTTTC 
CTGAACTTCG AGCAGGTTAA ATCCCTGATG TATTGGCTGG ATCCCAATCT TCGATATGCT 
ACACGAGAAG CGGACATCAT GGAGTATGCT GTGAACTGTC ATGTGATAAC CTGGGAGAGA 
ATCGTTAGTC ACTTTGATAT ATTTGCATTT GGCCATTTCT GGGGCTGGGC CATGAAGGCC 
TTGTTGATCC GTAGTTATGG GCTTTGCTGG ACAATCAGTA TCACCTGGGA GCTGACAGAG 
CTTTTCTTCA TGCATCTCCT GCCCAATTTT GCTGAGTGCT GGTGGGACCA AGTCATTCTG 
GACATCCTGT TGTGCAATGG TGGTGGAATC TGGCTGGGCA TGGTCGTTTG CCGGTTTCTG 
GAGATGAGAA CTTACCACTG GGCAAGCTTC AAGGACATCC ACACCACCAC AGGGAAGATC 
AAGCGAGCTG TGCTGCAGTT CACCCCTGCT AGCTGGACTT ACGTCAGGTG GTTTGATCCC 
AAGTCTTCCT TTCAGAGAGT GGCTGGTATA TACCTCTTCA TGATCATATG GCAGTTGACT 
GAGTTGAATA CCTTCTTCTT GAAGCACATC TTTGTGTTCC AAGCCAGCCA TCCCTTAAGC 
TGGGGTAGAA TCCTCTTCAT CGGCTGCATT ACGGCTCCCA CAGTGAGACA GTACTATGCC 
TACCTCACGG ACACACAGTG CAAGCGTGTA GGGACACAGT GCTGGGTGTT TGGGGTCATT 
GGTTTCCTGG AAGCTATTGT TTGCATAAAA TTTGGACAAG ATCTCTTTTC TAAGACCCAG 
ATACTCTATG TGATGCTTTG GCTTCTTTGT GTGGCCTTCA CCACGTTCCT GTGTCTGTAC 
GGCATGGTTT GGTATGCAGA GCACTATGGT CACCGGGAAA AGACCTATTC AGAGTGTGAA 
GATGGCACTT ACAGTCCAGA GATCTCCTGG CATCATGGGA AAGGCTCCAA AGGTTCTGAA 
GACAGCCCAC CGAAGCATTC AAGCAACCAC GAAAGCCATT CTTCCAGAAG AAGGAACCGG 
CATTCTAAGT CCAAAGTCAC TAATGGAGTT GGAAAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032985.2
CDS	679..2100
Translation	MASCVGSRTLSKDDVNYRMHFRMINEQQVEDITIDFFYRPHTITLLSFTIISLMYFAFTRDDSVPEDNIWRGILSVIFFFLIISVLAFPNGPFTRPHPALWRMVFGLSVLYFLFLVFLLFLNFEQVKSLMYWLDPNLRYATREADIMEYAVNCHVITWERIVSHFDIFAFGHFWGWAMKALLIRSYGLCWTISITWELTELFFMHLLPNFAECWWDQVILDILLCNGGGIWLGMVVCRFLEMRTYHWASFKDIHTTTGKIKRAVLQFTPASWTYVRWFDPKSSFQRVAGIYLFMIIWQLTELNTFFLKHIFVFQASHPLSWGRILFIGCITAPTVRQYYAYLTDTQCKRVGTQCWVFGVIGFLEAIVCIKFGQDLFSKTQILYVMLWLLCVAFTTFLCLYGMVWYAEHYGHREKTYSECEDGTYSPEISWHHGKGSKGSEDSPPKHSSNHESHSSRRRNRHSKSKVTNGVGKK

Target ORF information:

RefSeq Version	NM_008959.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylserine synthase 1 (Ptdss1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008959.3

ORF Insert Sequence:

ATGGCGTCCT GCGTGGGGAG CAGGACTCTG AGCAAGGATG ATGTGAACTA CAGGATGCAT 
TTCCGCATGA TCAACGAGCA GCAAGTGGAG GACATCACCA TAGACTTCTT CTACCGGCCG 
CACACCATCA CCCTGCTCAG CTTCACCATC ATCAGCCTCA TGTACTTCGC CTTCACCAGG 
GATGACTCTG TTCCGGAAGA CAACATCTGG AGAGGCATCC TGTCTGTTAT CTTCTTCTTT 
CTCATCATCA GCGTGTTAGC ATTCCCCAAT GGTCCATTTA CTCGACCTCA TCCAGCCTTA 
TGGCGAATGG TTTTTGGTCT CAGTGTGTTG TATTTCCTCT TCTTGGTATT CCTCCTTTTC 
CTGAACTTCG AGCAGGTTAA ATCCCTGATG TATTGGCTGG ATCCCAATCT TCGATATGCT 
ACACGAGAAG CGGACATCAT GGAGTATGCT GTGAACTGTC ATGTGATAAC CTGGGAGAGA 
ATCGTTAGTC ACTTTGATAT ATTTGCATTT GGCCATTTCT GGGGCTGGGC CATGAAGGCC 
TTGTTGATCC GTAGTTATGG GCTTTGCTGG ACAATCAGTA TCACCTGGGA GCTGACAGAG 
CTTTTCTTCA TGCATCTCCT GCCCAATTTT GCTGAGTGCT GGTGGGACCA AGTCATTCTG 
GACATCCTGT TGTGCAATGG TGGTGGAATC TGGCTGGGCA TGGTCGTTTG CCGGTTTCTG 
GAGATGAGAA CTTACCACTG GGCAAGCTTC AAGGACATCC ACACCACCAC AGGGAAGATC 
AAGCGAGCTG TGCTGCAGTT CACCCCTGCT AGCTGGACTT ACGTCAGGTG GTTTGATCCC 
AAGTCTTCCT TTCAGAGAGT GGCTGGTATA TACCTCTTCA TGATCATATG GCAGTTGACT 
GAGTTGAATA CCTTCTTCTT GAAGCACATC TTTGTGTTCC AAGCCAGCCA TCCCTTAAGC 
TGGGGTAGAA TCCTCTTCAT CGGCTGCATT ACGGCTCCCA CAGTGAGACA GTACTATGCC 
TACCTCACGG ACACACAGTG CAAGCGTGTA GGGACACAGT GCTGGGTGTT TGGGGTCATT 
GGTTTCCTGG AAGCTATTGT TTGCATAAAA TTTGGACAAG ATCTCTTTTC TAAGACCCAG 
ATACTCTATG TGATGCTTTG GCTTCTTTGT GTGGCCTTCA CCACGTTCCT GTGTCTGTAC 
GGCATGGTTT GGTATGCAGA GCACTATGGT CACCGGGAAA AGACCTATTC AGAGTGTGAA 
GATGGCACTT ACAGTCCAGA GATCTCCTGG CATCATGGGA AAGGCTCCAA AGGTTCTGAA 
GACAGCCCAC CGAAGCATTC AAGCAACCAC GAAAGCCATT CTTCCAGAAG AAGGAACCGG 
CATTCTAAGT CCAAAGTCAC TAATGGAGTT GGAAAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells.
The Journal of biological chemistry286(2)1061-73(2011 Jan)
Tasseva G,Cole L,Vance JE

Defining the importance of phosphatidylserine synthase-1 (PSS1): unexpected viability of PSS1-deficient mice.
The Journal of biological chemistry283(19)12888-97(2008 May)
Arikketh D,Nelson R,Vance JE

Identification of transcripts with enriched expression in the developing and adult pancreas.
Genome biology9(6)R99(2008)
Hoffman BG,Zavaglia B,Witzsche J,Ruiz de Algara T,Beach M,Hoodless PA,Jones SJ,Marra MA,Helgason CD

Synthesis of phosphatidylserine by base exchange in Triton-insoluble floating fractions from rat cerebellum.
Journal of neurochemistry103(3)942-51(2007 Nov)
Buratta S,Felicetti M,Mozzi R