Ppt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppt1
Entrez Gene ID	19063
Full Name	palmitoyl-protein thioesterase 1
Synonyms	9530043G02Rik,AA960502,C77813,CLN1,D4Ertd184e,INCL,PPT
General protein information	Preferred Names palmitoyl-protein thioesterase 1 Names palmitoyl-protein thioesterase 1 PPT-1 palmitoyl-protein hydrolase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D2.2\|4 57.27 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008917.3	NP_032943.2	palmitoyl-protein thioesterase 1 precursor

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ppt1	NP_727284.1
Caenorhabditis elegans (roundworm)	ppt-1	NP_504684.2
Oryza sativa (rice)	Os03g0101100	NP_001048650.1
	PPT1	XP_001082462.1
Mus musculus (house mouse)	Ppt1	NP_032943.2
Schizosaccharomyces pombe (fission yeast)	pdf1	NP_595325.2
Magnaporthe oryzae (rice blast fungus)	MGG_04920	XP_003712411.1
Arabidopsis thaliana (thale cress)	AT3G60340	NP_850728.1
Homo sapiens (human)	PPT1	NP_000301.1
Canis lupus familiaris (dog)	PPT1	NP_001010944.1
Bos taurus (cattle)	PPT1	NP_776579.1
Rattus norvegicus (Norway rat)	Ppt1	NP_071947.1
Danio rerio (zebrafish)	ppt1	NP_998504.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001058	XP_311781.4
Pan troglodytes (chimpanzee)	PPT1	XP_001172215.1
Gallus gallus (chicken)	PPT1	NP_001026074.1
Neurospora crassa	NCU06530	XP_961802.1
Oryza sativa (rice)	Os10g0563000	NP_001065397.2
Xenopus tropicalis (tropical clawed frog)	ppt1	NP_001006875.1

Gene Ontology

The following Ppt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22793	NM_008917.3 Latest version!	Mus musculus palmitoyl-protein thioesterase 1 (Ppt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppt1 ( NM_008917.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032943.2 Mus musculus palmitoyl-protein thioesterase 1 (Ppt1), mRNA.

Price & Availability↑

CloneID	OMu22793
Clone ID Related Accession (Same CDS sequence)	NM_008917.3
Accession Version	NM_008917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	palmitoyl-protein thioesterase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB600397.1, AK151107.1 and AK169579.1. On Dec 10, 2010 this sequence version replaced NM_008917.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK014561.1, AK152940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCGT CCTGTTCGCG GAGGCTGCTA GCTGCCGCTC TGCTGCCTTG GTGCTGCGCC 
GCCTGGGCCC TGGGGCATCT GGACCCACCT TCACCACCAC CGCTGGTGAT CTGGCATGGG 
ATGGGGGACA GCTGTTGTAA CCCCATGAGC ATGGGTGTCA TTAAAAAGAT GGTTGAAAAA 
GAAATACCTG GGATTTACGT CCTGTCTCTA GAGATTGGGA AGAACATGAT GGAGGATGTG 
GAGAACAGCT TCTTCTTGAA TGTTAATGTC CAAGTCAACA TGGTGTGTCA GATTCTGGAA 
AAGGATCCTA AGTTGCAGCA GGGATACAAT GCTATTGGCT TCTCCCAGGG AGGCCAGTTC 
CTGAGGGCAG TGGCTCAGAG ATGCCCAACA CCTCCCATGA TGACCCTGAT CTCAGTTGGA 
GGACAACATC AAGGTGTCTT TGGACTCCCC CGATGCCCAG GAGAGAGTTC TCACATCTGC 
GACTTCATCA GGAAGTCACT TAATGCCGGT GCTTACTCCA AACTTGTGCA AGAACGCCTG 
GTGCAAGCAC AGTACTGGCA TGACCCTATC AAGGAGAGTG TGTACCGAAA CTACAGCATC 
TTCTTGGCAG ACATAAATCA AGAGAGGTGT GTCAATGAGT CCTACAAGAA GAACCTGATG 
GCCCTCAAGA AGTTTGTGAT GGTGAAATTC TTTAATGATT CCATTGTGGA CCCTGTCGAC 
TCTGAGTGGT TTGGATTTTA CAGAAGTGGC CAAGCTAAGG AAACCATTCC CCTCCAGGAG 
AGCACTCTAT ACACAGAGGA CCGCCTGGGG CTAAAGAAAA TGGACAAAGC AGGAAAGCTA 
GTGTTTCTGG CTAAGGAAGG GGACCATCTT CAAATATCTA AAGAATGGTT TACTGCCCAC 
ATCATACCTT TTCTTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032943.2
CDS	30..950
Translation	MASSCSRRLLAAALLPWCCAAWALGHLDPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK

Target ORF information:

RefSeq Version	NM_008917.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus palmitoyl-protein thioesterase 1 (Ppt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008917.3

ORF Insert Sequence:

ATGGCGTCGT CCTGTTCGCG GAGGCTGCTA GCTGCCGCTC TGCTGCCTTG GTGCTGCGCC 
GCCTGGGCCC TGGGGCATCT GGACCCACCT TCACCACCAC CGCTGGTGAT CTGGCATGGG 
ATGGGGGACA GCTGTTGTAA CCCCATGAGC ATGGGTGTCA TTAAAAAGAT GGTTGAAAAA 
GAAATACCTG GGATTTACGT CCTGTCTCTA GAGATTGGGA AGAACATGAT GGAGGATGTG 
GAGAACAGCT TCTTCTTGAA TGTTAATGTC CAAGTCAACA TGGTGTGTCA GATTCTGGAA 
AAGGATCCTA AGTTGCAGCA GGGATACAAT GCTATTGGCT TCTCCCAGGG AGGCCAGTTC 
CTGAGGGCAG TGGCTCAGAG ATGCCCAACA CCTCCCATGA TGACCCTGAT CTCAGTTGGA 
GGACAACATC AAGGTGTCTT TGGACTCCCC CGATGCCCAG GAGAGAGTTC TCACATCTGC 
GACTTCATCA GGAAGTCACT TAATGCCGGT GCTTACTCCA AACTTGTGCA AGAACGCCTG 
GTGCAAGCAC AGTACTGGCA TGACCCTATC AAGGAGAGTG TGTACCGAAA CTACAGCATC 
TTCTTGGCAG ACATAAATCA AGAGAGGTGT GTCAATGAGT CCTACAAGAA GAACCTGATG 
GCCCTCAAGA AGTTTGTGAT GGTGAAATTC TTTAATGATT CCATTGTGGA CCCTGTCGAC 
TCTGAGTGGT TTGGATTTTA CAGAAGTGGC CAAGCTAAGG AAACCATTCC CCTCCAGGAG 
AGCACTCTAT ACACAGAGGA CCGCCTGGGG CTAAAGAAAA TGGACAAAGC AGGAAAGCTA 
GTGTTTCTGG CTAAGGAAGG GGACCATCTT CAAATATCTA AAGAATGGTT TACTGCCCAC 
ATCATACCTT TTCTTAAGTG A