Cfap20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cfap20
Entrez Gene ID	14894
Full Name	cilia and flagella associated protein 20
Synonyms	2600014O15Rik,AL024127,Gtl3,T10-2A2
General protein information	Preferred Names cilia and flagella associated protein 20 Names cilia- and flagella-associated protein 20 UPF0468 protein C16orf80 homolog gene trap locus 3 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_008187.2	NP_032213.2	cilia- and flagella-associated protein 20

Pathways from BioSystems

Homologies

Homo sapiens (human)	C16orf80	NP_037374.1
Bos taurus (cattle)	C18H16orf80	NP_001003905.1
Canis lupus familiaris (dog)	C2H16orf80	XP_544382.2
Mus musculus (house mouse)	Gtl3	NP_032213.2
Pan troglodytes (chimpanzee)	C16H16orf80	XP_511001.1
Danio rerio (zebrafish)	zgc:73380	NP_957105.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009680	XP_318737.3
Caenorhabditis elegans (roundworm)	C54C6.6	NP_871706.1
	C20H16orf80	XP_001101318.1
Gallus gallus (chicken)	C11H16ORF80	NP_001005833.1
Oryza sativa (rice)	Os09g0510200	NP_001063633.1
Drosophila melanogaster (fruit fly)	CG5343	NP_609402.1
Arabidopsis thaliana (thale cress)	AT3G12300	NP_566418.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic analysis of mammalian primary cilia.
Ishikawa H, Thompson J, Yates JR 3rd, Marshall WF
Current biology : CB22(5)414-9(2012 Mar)

High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization.
Yoshikawa T, Piao Y, Zhong J, Matoba R, Carter MG, Wang Y, Goldberg I, Ko MS
Gene expression patterns : GEP6(2)213-24(2006 Jan)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Sequence and expression pattern of an evolutionarily conserved transcript identified by gene trapping.
Rijkers T, R?ther U
Biochimica et biophysica acta1307(3)294-300(1996 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cfap20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cfap20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01495	NM_008187.2 Latest version!	Mus musculus cilia and flagella associated protein 20 (Cfap20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cfap20 ( NM_008187.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032213.2 Mus musculus cilia and flagella associated protein 20 (Cfap20), mRNA.

Price & Availability↑

CloneID	OMu01495
Clone ID Related Accession (Same CDS sequence)	NM_008187.2
Accession Version	NM_008187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cilia- and flagella-associated protein 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM934726.1, BC119023.1 and CB195345.1. On Mar 21, 2009 this sequence version replaced NM_008187.1. ##Evidence-Data-START## Transcript exon combination :: AK011217.1, Z54179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTTCAAGA ACACGTTCCA GAGTGGCTTC CTTTCCATCC TCTACAGCAT CGGCAGCAAG 
CCCCTGCAGA TCTGGGACAA AAAGGTACGG AATGGCCACA TCAAAAGAAT CACTGACAAT 
GACATCCAGT CCCTGGTGCT AGAGATTGAA GGGACAAATG TCAGTACCAC ATACATCACG 
TGTCCTGCGG ACCCCAAGAA GACACTGGGG ATCAAACTGC CTTTCCTTGT CATGATCATC 
AAAAACTTGA AGAAATATTT TACCTTTGAA GTCCAGGTAC TAGATGACAA AAACGTTCGT 
CGAAGGTTTC GAGCAAGTAA CTACCAGAGT ACCACTCGAG TCAAGCCCTT CATCTGTACC 
ATGCCCATGC GGCTGGATGA TGGCTGGAAC CAGATTCAGT TCAACTTGTC AGATTTCACC 
CGGCGGGCAT ATGGCACGAA CTACATCGAG ACCCTGAGAG TGCAGATCCA TGCAAACTGC 
CGTATCCGAA GGGTTTACTT CTCAGACAGA CTCTACTCAG AAGATGAGCT GCCAGCAGAG 
TTCAAACTCT ATCTACCAGT TCAGAACAAG GCCAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032213.2
CDS	299..880
Translation	MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKAKQ

Target ORF information:

RefSeq Version	NM_008187.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cilia and flagella associated protein 20 (Cfap20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008187.2

ORF Insert Sequence:

ATGTTCAAGA ACACGTTCCA GAGTGGCTTC CTTTCCATCC TCTACAGCAT CGGCAGCAAG 
CCCCTGCAGA TCTGGGACAA AAAGGTACGG AATGGCCACA TCAAAAGAAT CACTGACAAT 
GACATCCAGT CCCTGGTGCT AGAGATTGAA GGGACAAATG TCAGTACCAC ATACATCACG 
TGTCCTGCGG ACCCCAAGAA GACACTGGGG ATCAAACTGC CTTTCCTTGT CATGATCATC 
AAAAACTTGA AGAAATATTT TACCTTTGAA GTCCAGGTAC TAGATGACAA AAACGTTCGT 
CGAAGGTTTC GAGCAAGTAA CTACCAGAGT ACCACTCGAG TCAAGCCCTT CATCTGTACC 
ATGCCCATGC GGCTGGATGA TGGCTGGAAC CAGATTCAGT TCAACTTGTC AGATTTCACC 
CGGCGGGCAT ATGGCACGAA CTACATCGAG ACCCTGAGAG TGCAGATCCA TGCAAACTGC 
CGTATCCGAA GGGTTTACTT CTCAGACAGA CTCTACTCAG AAGATGAGCT GCCAGCAGAG 
TTCAAACTCT ATCTACCAGT TCAGAACAAG GCCAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic analysis of mammalian primary cilia.
Current biology : CB22(5)414-9(2012 Mar)
Ishikawa H,Thompson J,Yates JR 3rd,Marshall WF

High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization.
Gene expression patterns : GEP6(2)213-24(2006 Jan)
Yoshikawa T,Piao Y,Zhong J,Matoba R,Carter MG,Wang Y,Goldberg I,Ko MS

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Sequence and expression pattern of an evolutionarily conserved transcript identified by gene trapping.
Biochimica et biophysica acta1307(3)294-300(1996 Jul)
Rijkers T,R?ther U