Gcsam cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcsam
Entrez Gene ID	14525
Full Name	germinal center associated, signaling and motility
Synonyms	Gcet,Gcet2,M17,M17-L
General protein information	Preferred Names germinal center associated, signaling and motility Names germinal center-associated signaling and motility protein germinal center B-cell-expressed transcript 2 protein germinal center expressed transcript 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159297.1	NP_001152769.1	germinal center-associated signaling and motility protein isoform 1
NM_008099.4	NP_032125.3	germinal center-associated signaling and motility protein isoform 2

Pathways from BioSystems

Homologies

	GCSAM	XP_001103280.1
Mus musculus (house mouse)	Gcsam	NP_001152769.1
Bos taurus (cattle)	LOC784939	NP_001178137.1
Rattus norvegicus (Norway rat)	Gcsam	XP_001064866.2
Homo sapiens (human)	GCSAM	NP_001177188.1
Pan troglodytes (chimpanzee)	GCSAM	XP_001154677.1
Canis lupus familiaris (dog)	GCSAM	XP_005639532.1

Gene Ontology
Bibliography

Related articles in PubMed

M17, a gene specific for germinal center (GC) B cells and a prognostic marker for GC B-cell lymphomas, is dispensable for the GC reaction in mice.
Schenten D, Egert A, Pasparakis M, Rajewsky K
Blood107(12)4849-56(2006 Jun)

Identification of an Sp factor-dependent promoter in GCET, a gene expressed at high levels in germinal center B cells.
Steinke JW, Hodsdon W, Parenti S, Ostraat R, Lutz R, Borish L, Hagman J
Molecular immunology41(12)1145-53(2004 Nov)

Protocol for using signal peptide differential display and representational difference analysis to isolate differentially expressed cDNAs from fetal mouse brain.
Eriksson A, Nordqvist K
Brain research. Brain research protocols6(3)119-28(2001 Feb)

M17: a novel gene expressed in germinal centers.
Christoph T, Rickert R, Rajewsky K
International immunology6(8)1203-11(1994 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Assays identified a non-consensus binding site for Sp factors near sites of transcriptional initiation. The site binds Spl and Sp3 in nuclear extracts.
Title: Identification of an Sp factor-dependent promoter in GCET, a gene expressed at high levels in germinal center B cells.

The following Gcsam gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcsam cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01357	NM_008099.4 Latest version!	Mus musculus germinal center associated, signaling and motility (Gcsam), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu07367	NM_001159297.1 Latest version!	Mus musculus germinal center associated, signaling and motility (Gcsam), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcsam ( NM_008099.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032125.3 Mus musculus germinal center associated, signaling and motility (Gcsam), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01357
Clone ID Related Accession (Same CDS sequence)	NM_008099.4
Accession Version	NM_008099.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	germinal center-associated signaling and motility protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U13263.1 and AK036553.1. On Apr 11, 2009 this sequence version replaced NM_008099.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This difference results in a protein (isoform 2) with a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK036553.1, U13263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAACT GTTTGCAGAG GACAACCAGA TACTTCAGGG GCTGGAGTTG TTGTCACAGC 
GTTGAAGGAT GCTCCTGCCT TCCTTGGAAA AACATACGCA CATTTAAAGC CAGACAAGAG 
TCTCCAAAGC AAAATGAAGG GATGACTTCA GCTCCCGTTC AGGACAATGC TAATGAGACC 
TACACAGAAG AGTTGTGCTA CATCCTTGTT GATCACGAAG CTGTCAGGGG AAGGCCATCA 
GTGAACCCTG CTGAGGGGTT CTACGAGAAC ATCTCTAACA AGGCTGAGAG ACACAAAGAG 
TCTTCAAGAG GAACAGAGAC TGAGTATTCG GTTCTCCGTT TCCCTTCTCC CCCTCAGCCC 
CTACCTTCCA CAGATGATGA ATATGAACTT CTTATGCCCA GCAGATTCTC CTCACATGCC 
TTTCAACAGC CACGGCCGCT TACAACCCCC TACGAGACAC ACTTTTCTTA TCCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032125.3
CDS	93..572
Translation	MGNCLQRTTRYFRGWSCCHSVEGCSCLPWKNIRTFKARQESPKQNEGMTSAPVQDNANETYTEELCYILVDHEAVRGRPSVNPAEGFYENISNKAERHKESSRGTETEYSVLRFPSPPQPLPSTDDEYELLMPSRFSSHAFQQPRPLTTPYETHFSYPQ

Target ORF information:

RefSeq Version	NM_008099.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus germinal center associated, signaling and motility (Gcsam), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008099.4

ORF Insert Sequence:

ATGGGGAACT GTTTGCAGAG GACAACCAGA TACTTCAGGG GCTGGAGTTG TTGTCACAGC 
GTTGAAGGAT GCTCCTGCCT TCCTTGGAAA AACATACGCA CATTTAAAGC CAGACAAGAG 
TCTCCAAAGC AAAATGAAGG GATGACTTCA GCTCCCGTTC AGGACAATGC TAATGAGACC 
TACACAGAAG AGTTGTGCTA CATCCTTGTT GATCACGAAG CTGTCAGGGG AAGGCCATCA 
GTGAACCCTG CTGAGGGGTT CTACGAGAAC ATCTCTAACA AGGCTGAGAG ACACAAAGAG 
TCTTCAAGAG GAACAGAGAC TGAGTATTCG GTTCTCCGTT TCCCTTCTCC CCCTCAGCCC 
CTACCTTCCA CAGATGATGA ATATGAACTT CTTATGCCCA GCAGATTCTC CTCACATGCC 
TTTCAACAGC CACGGCCGCT TACAACCCCC TACGAGACAC ACTTTTCTTA TCCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcsam ( NM_001159297.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152769.1 Mus musculus germinal center associated, signaling and motility (Gcsam), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07367
Clone ID Related Accession (Same CDS sequence)	NM_001159297.1
Accession Version	NM_001159297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	germinal center-associated signaling and motility protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U13263.1, AY500830.1 and AK036553.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AY500830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAACT GTTTGCAGAG GACAACCAGA TGGCAGCTGG ACATGCAGGA AACACCTTGG 
AATCTGAGAC TCAGTGCCAA GGGAAGAACA TGCAGATACT TCAGGGGCTG GAGTTGTTGT 
CACAGCGTTG AAGGATGCTC CTGCCTTCCT TGGAAAAACA TACGCACATT TAAAGCCAGA 
CAAGAGTCTC CAAAGCAAAA TGAAGGGATG ACTTCAGCTC CCGTTCAGGA CAATGCTAAT 
GAGACCTACA CAGAAGAGTT GTGCTACATC CTTGTTGATC ACGAAGCTGT CAGGGGAAGG 
CCATCAGTGA ACCCTGCTGA GGGGTTCTAC GAGAACATCT CTAACAAGGC TGAGAGACAC 
AAAGAGTCTT CAAGAGGAAC AGAGACTGAG TATTCGGTTC TCCGTTTCCC TTCTCCCCCT 
CAGCCCCTAC CTTCCACAGA TGATGAATAT GAACTTCTTA TGCCCAGCAG ATTCTCCTCA 
CATGCCTTTC AACAGCCACG GCCGCTTACA ACCCCCTACG AGACACACTT TTCTTATCCA 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152769.1
CDS	93..638
Translation	MGNCLQRTTRWQLDMQETPWNLRLSAKGRTCRYFRGWSCCHSVEGCSCLPWKNIRTFKARQESPKQNEGMTSAPVQDNANETYTEELCYILVDHEAVRGRPSVNPAEGFYENISNKAERHKESSRGTETEYSVLRFPSPPQPLPSTDDEYELLMPSRFSSHAFQQPRPLTTPYETHFSYPQ

Target ORF information:

RefSeq Version	NM_001159297.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus germinal center associated, signaling and motility (Gcsam), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159297.1

ORF Insert Sequence:

ATGGGGAACT GTTTGCAGAG GACAACCAGA TGGCAGCTGG ACATGCAGGA AACACCTTGG 
AATCTGAGAC TCAGTGCCAA GGGAAGAACA TGCAGATACT TCAGGGGCTG GAGTTGTTGT 
CACAGCGTTG AAGGATGCTC CTGCCTTCCT TGGAAAAACA TACGCACATT TAAAGCCAGA 
CAAGAGTCTC CAAAGCAAAA TGAAGGGATG ACTTCAGCTC CCGTTCAGGA CAATGCTAAT 
GAGACCTACA CAGAAGAGTT GTGCTACATC CTTGTTGATC ACGAAGCTGT CAGGGGAAGG 
CCATCAGTGA ACCCTGCTGA GGGGTTCTAC GAGAACATCT CTAACAAGGC TGAGAGACAC 
AAAGAGTCTT CAAGAGGAAC AGAGACTGAG TATTCGGTTC TCCGTTTCCC TTCTCCCCCT 
CAGCCCCTAC CTTCCACAGA TGATGAATAT GAACTTCTTA TGCCCAGCAG ATTCTCCTCA 
CATGCCTTTC AACAGCCACG GCCGCTTACA ACCCCCTACG AGACACACTT TTCTTATCCA 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

M17, a gene specific for germinal center (GC) B cells and a prognostic marker for GC B-cell lymphomas, is dispensable for the GC reaction in mice.
Blood107(12)4849-56(2006 Jun)
Schenten D,Egert A,Pasparakis M,Rajewsky K

Identification of an Sp factor-dependent promoter in GCET, a gene expressed at high levels in germinal center B cells.
Molecular immunology41(12)1145-53(2004 Nov)
Steinke JW,Hodsdon W,Parenti S,Ostraat R,Lutz R,Borish L,Hagman J

Protocol for using signal peptide differential display and representational difference analysis to isolate differentially expressed cDNAs from fetal mouse brain.
Brain research. Brain research protocols6(3)119-28(2001 Feb)
Eriksson A,Nordqvist K

M17: a novel gene expressed in germinal centers.
International immunology6(8)1203-11(1994 Aug)
Christoph T,Rickert R,Rajewsky K