Csn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Csn2
Entrez Gene ID	12991
Full Name	casein beta
Synonyms	Csnb
General protein information	Preferred Names casein beta Names beta-casein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 E1\|5 43.56 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009972.2	NP_034102.1	beta-casein isoform a precursor
NM_001286020.1	NP_001272949.1	beta-casein isoform b precursor
NM_001286021.1	NP_001272950.1	beta-casein isoform b precursor
NM_001286024.1	NP_001272953.1	beta-casein isoform e precursor
NM_001286022.1	NP_001272951.1	beta-casein isoform c precursor
NM_001286023.1	NP_001272952.1	beta-casein isoform d precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Csn2	NP_058816.2
	CSN2	XP_001095936.2
Canis lupus familiaris (dog)	CSN2	NP_001003086.1
Mus musculus (house mouse)	Csn2	NP_034102.1
Bos taurus (cattle)	CSN2	NP_851351.1
Homo sapiens (human)	CSN2	NP_001882.1
Pan troglodytes (chimpanzee)	CSN2	XP_001143844.2

Gene Ontology
Bibliography

Related articles in PubMed

Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in ?-casein secretion pathway in lactating mammary epithelial cells.
Kobayashi K, Oyama S, Kuki C, Tsugami Y, Matsunaga K, Suzuki T, Nishimura T
Molecular and cellular endocrinology44016-24(2017 Jan)

Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with ?-casein expression in mammary epithelial cells.
Kobayashi K, Tsugami Y, Matsunaga K, Oyama S, Kuki C, Kumura H
Biochimica et biophysica acta1863(8)2006-16(2016 08)

Interactions of the ubiquitous octamer-binding transcription factor-1 with both the signal transducer and activator of transcription 5 and the glucocorticoid receptor mediate prolactin and glucocorticoid-induced ?-casein gene expression in mammary epithelial cells.
Qian X, Zhao FQ
The international journal of biochemistry & cell biology45(3)724-35(2013 Mar)

The Stat6-regulated KRAB domain zinc finger protein Zfp157 regulates the balance of lineages in mammary glands and compensates for loss of Gata-3.
Oliver CH, Khaled WT, Frend H, Nichols J, Watson CJ
Genes & development26(10)1086-97(2012 May)

Insulin-like growth factors 1 and 2 regulate expression of ?-casein in vitro in mouse mammary epithelial cells.
Shushanov SS
Bulletin of experimental biology and medicine152(2)202-5(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway.
Title: Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in ?-casein secretion pathway in lactating mammary epithelial cells.

Dexamethasone signaling upregulates Claudin-3 and -4 without beta-casein expression. Prolactin and dexamethasone signaling synergistically upregulate beta-casein expression.
Title: Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with ?-casein expression in mammary epithelial cells.

octamer-binding transcription factor-1 may serve as a master regulator that facilitates the DNA binding of both signal transducer and activator of transcription 5 and glucocorticoid receptor in hormone-induced beta-casein expression
Title: Interactions of the ubiquitous octamer-binding transcription factor-1 with both the signal transducer and activator of transcription 5 and the glucocorticoid receptor mediate prolactin and glucocorticoid-induced ?-casein gene expression in mammary epithelial cells.

It was found that both IGF-1 and IGF-2 activate the expression of milk protein beta-casein in the presence of prolactin and hydrocortisone. It was found that beta-casein expression is accompanied by cyclin D1 coexpression.
Title: Insulin-like growth factors 1 and 2 regulate expression of ?-casein in vitro in mouse mammary epithelial cells.

mammary cells express beta-casein in response to prolactin in a pathway that involves gap junction intracellular communication and OCT-1 and is independent of STAT5 nuclear translocation
Title: Gap junctions mediate STAT5-independent ?-casein expression in CID-9 mammary epithelial cells.

The following Csn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Csn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05244	NM_009972.2 Latest version!	Mus musculus casein beta (Csn2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu09152	NM_001286022.1 Latest version!	Mus musculus casein beta (Csn2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu09154	NM_001286023.1 Latest version!	Mus musculus casein beta (Csn2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu09155	NM_001286024.1 Latest version!	Mus musculus casein beta (Csn2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu09150	NM_001286021.1 Latest version!	Mus musculus casein beta (Csn2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu09150	NM_001286020.1 Latest version!	Mus musculus casein beta (Csn2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Csn2 ( NM_009972.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034102.1 Mus musculus casein beta (Csn2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05244
Clone ID Related Accession (Same CDS sequence)	NM_009972.2
Accession Version	NM_009972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	beta-casein isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074046.9 and AK052805.1. On Oct 26, 2013 this sequence version replaced NM_009972.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052805.1, X04490.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTACATTT 
ACTGTATCCT CTGAGACTGA TAGTATTTCC AGTGAGGAAT CTGTTGAACA TATCAATGAG 
CAGAAACTTC AGAAGGTGAA TCTCATGGGA CAGCTGCAGG CAGAGGATGT GCTCCAGGCT 
AAAGTTCACT CCAGCATCCA GTCACAGCCC CAGGCCTTTC CATATGCTCA GGCTCAAACC 
ATCTCTTGCA ATCCCGTCCC ACAAAACATC CAGCCTATTG CTCAACCCCC TGTGGTGCCA 
TCTCTTGGGC CTGTCATTTC TCCTGAACTG GAATCCTTCC TTAAAGCTAA AGCCACCATC 
CTTCCCAAGC ACAAACAGAT GCCCCTCCTT AACTCTGAAA CTGTGCTCCG CCTCATAAAC 
TCTCAAATCC CCAGCCTTGC CAGTCTTGCT AATCTGCACC TTCCTCAGTC TCTGGTCCAG 
CTCCTGGCAC AGGTTGTTCA GGCTTTTCCT CAGACTCACC TGGTTTCTTC TCAGACCCAG 
CTGTCTCTTC CTCAGTCCAA AGTTCTGTAC TTTCTGCAGC AAGTAGCACC CTTCCTTCCA 
CAAGATATGT CTGTCCAAGA CCTTCTGCAG TACCTAGAAC TTCTTAACCC CACCGTCCAA 
TTCCCTGCCA CTCCACAACA TTCCGTTTCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034102.1
CDS	60..755
Translation	MKVFILACLVALALARETTFTVSSETDSISSEESVEHINEQKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAFPQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQHSVSV

Target ORF information:

RefSeq Version	NM_009972.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus casein beta (Csn2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009972.2

ORF Insert Sequence:

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTACATTT 
ACTGTATCCT CTGAGACTGA TAGTATTTCC AGTGAGGAAT CTGTTGAACA TATCAATGAG 
CAGAAACTTC AGAAGGTGAA TCTCATGGGA CAGCTGCAGG CAGAGGATGT GCTCCAGGCT 
AAAGTTCACT CCAGCATCCA GTCACAGCCC CAGGCCTTTC CATATGCTCA GGCTCAAACC 
ATCTCTTGCA ATCCCGTCCC ACAAAACATC CAGCCTATTG CTCAACCCCC TGTGGTGCCA 
TCTCTTGGGC CTGTCATTTC TCCTGAACTG GAATCCTTCC TTAAAGCTAA AGCCACCATC 
CTTCCCAAGC ACAAACAGAT GCCCCTCCTT AACTCTGAAA CTGTGCTCCG CCTCATAAAC 
TCTCAAATCC CCAGCCTTGC CAGTCTTGCT AATCTGCACC TTCCTCAGTC TCTGGTCCAG 
CTCCTGGCAC AGGTTGTTCA GGCTTTTCCT CAGACTCACC TGGTTTCTTC TCAGACCCAG 
CTGTCTCTTC CTCAGTCCAA AGTTCTGTAC TTTCTGCAGC AAGTAGCACC CTTCCTTCCA 
CAAGATATGT CTGTCCAAGA CCTTCTGCAG TACCTAGAAC TTCTTAACCC CACCGTCCAA 
TTCCCTGCCA CTCCACAACA TTCCGTTTCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csn2 ( NM_001286022.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272951.1 Mus musculus casein beta (Csn2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu09152
Clone ID Related Accession (Same CDS sequence)	NM_001286022.1
Accession Version	NM_001286022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	beta-casein isoform c precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074046.9 and AK085698.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849383 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTGATAGT 
ATTTCCAGTG AGGAATCTGT TGAACATATC AATGAGAAAC TTCAGAAGGT GAATCTCATG 
GGACAGCTGC AGGCAGAGGA TGTGCTCCAG GCTAAAGTTC ACTCCAGCAT CCAGTCACAG 
CCCCAGGCCT TTCCATATGC TCAGGCTCAA ACCATCTCTT GCAATCCCGT CCCACAAAAC 
ATCCAGCCTA TTGCTCAACC CCCTGTGGTG CCATCTCTTG GGCCTGTCAT TTCTCCTGAA 
CTGGAATCCT TCCTTAAAGC TAAAGCCACC ATCCTTCCCA AGCACAAACA GATGCCCCTC 
CTTAACTCTG AAACTGTGCT CCGCCTCATA AACTCTCAAA TCCCCAGCCT TGCCAGTCTT 
GCTAATCTGC ACCTTCCTCA GTCTCTGGTC CAGCTCCTGG CACAGGTTGT TCAGGCTTTT 
CCTCAGACTC ACCTGGTTTC TTCTCAGACC CAGCTGTCTC TTCCTCAGTC CAAAGTTCTG 
TACTTTCTGC AGCAAGTAGC ACCCTTCCTT CCACAAGATA TGTCTGTCCA AGACCTTCTG 
CAGTACCTAG AACTTCTTAA CCCCACCGTC CAATTCCCTG CCACTCCACA ACATTCCGTT 
TCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272951.1
CDS	60..728
Translation	MKVFILACLVALALARETDSISSEESVEHINEKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAFPQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQHSVSV

Target ORF information:

RefSeq Version	NM_001286022.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus casein beta (Csn2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286022.1

ORF Insert Sequence:

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTGATAGT 
ATTTCCAGTG AGGAATCTGT TGAACATATC AATGAGAAAC TTCAGAAGGT GAATCTCATG 
GGACAGCTGC AGGCAGAGGA TGTGCTCCAG GCTAAAGTTC ACTCCAGCAT CCAGTCACAG 
CCCCAGGCCT TTCCATATGC TCAGGCTCAA ACCATCTCTT GCAATCCCGT CCCACAAAAC 
ATCCAGCCTA TTGCTCAACC CCCTGTGGTG CCATCTCTTG GGCCTGTCAT TTCTCCTGAA 
CTGGAATCCT TCCTTAAAGC TAAAGCCACC ATCCTTCCCA AGCACAAACA GATGCCCCTC 
CTTAACTCTG AAACTGTGCT CCGCCTCATA AACTCTCAAA TCCCCAGCCT TGCCAGTCTT 
GCTAATCTGC ACCTTCCTCA GTCTCTGGTC CAGCTCCTGG CACAGGTTGT TCAGGCTTTT 
CCTCAGACTC ACCTGGTTTC TTCTCAGACC CAGCTGTCTC TTCCTCAGTC CAAAGTTCTG 
TACTTTCTGC AGCAAGTAGC ACCCTTCCTT CCACAAGATA TGTCTGTCCA AGACCTTCTG 
CAGTACCTAG AACTTCTTAA CCCCACCGTC CAATTCCCTG CCACTCCACA ACATTCCGTT 
TCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csn2 ( NM_001286023.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272952.1 Mus musculus casein beta (Csn2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu09154
Clone ID Related Accession (Same CDS sequence)	NM_001286023.1
Accession Version	NM_001286023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	beta-casein isoform d precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074046.9 and AK052805.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons in the 5' coding region, compared to variant 1, resulting in an isoform (d) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI251319.1, BG246313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GGAATCTGTT 
GAACATATCA ATGAGCAGAA ACTTCAGAAG GTGAATCTCA TGGGACAGCT GCAGGCAGAG 
GATGTGCTCC AGGCTAAAGT TCACTCCAGC ATCCAGTCAC AGCCCCAGGC CTTTCCATAT 
GCTCAGGCTC AAACCATCTC TTGCAATCCC GTCCCACAAA ACATCCAGCC TATTGCTCAA 
CCCCCTGTGG TGCCATCTCT TGGGCCTGTC ATTTCTCCTG AACTGGAATC CTTCCTTAAA 
GCTAAAGCCA CCATCCTTCC CAAGCACAAA CAGATGCCCC TCCTTAACTC TGAAACTGTG 
CTCCGCCTCA TAAACTCTCA AATCCCCAGC CTTGCCAGTC TTGCTAATCT GCACCTTCCT 
CAGTCTCTGG TCCAGCTCCT GGCACAGGTT GTTCAGGCTT TTCCTCAGAC TCACCTGGTT 
TCTTCTCAGA CCCAGCTGTC TCTTCCTCAG TCCAAAGTTC TGTACTTTCT GCAGCAAGTA 
GCACCCTTCC TTCCACAAGA TATGTCTGTC CAAGACCTTC TGCAGTACCT AGAACTTCTT 
AACCCCACCG TCCAATTCCC TGCCACTCCA CAACATTCCG TTTCTGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272952.1
CDS	60..710
Translation	MKVFILACLVALALAREESVEHINEQKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAFPQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQHSVSV

Target ORF information:

RefSeq Version	NM_001286023.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus casein beta (Csn2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286023.1

ORF Insert Sequence:

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GGAATCTGTT 
GAACATATCA ATGAGCAGAA ACTTCAGAAG GTGAATCTCA TGGGACAGCT GCAGGCAGAG 
GATGTGCTCC AGGCTAAAGT TCACTCCAGC ATCCAGTCAC AGCCCCAGGC CTTTCCATAT 
GCTCAGGCTC AAACCATCTC TTGCAATCCC GTCCCACAAA ACATCCAGCC TATTGCTCAA 
CCCCCTGTGG TGCCATCTCT TGGGCCTGTC ATTTCTCCTG AACTGGAATC CTTCCTTAAA 
GCTAAAGCCA CCATCCTTCC CAAGCACAAA CAGATGCCCC TCCTTAACTC TGAAACTGTG 
CTCCGCCTCA TAAACTCTCA AATCCCCAGC CTTGCCAGTC TTGCTAATCT GCACCTTCCT 
CAGTCTCTGG TCCAGCTCCT GGCACAGGTT GTTCAGGCTT TTCCTCAGAC TCACCTGGTT 
TCTTCTCAGA CCCAGCTGTC TCTTCCTCAG TCCAAAGTTC TGTACTTTCT GCAGCAAGTA 
GCACCCTTCC TTCCACAAGA TATGTCTGTC CAAGACCTTC TGCAGTACCT AGAACTTCTT 
AACCCCACCG TCCAATTCCC TGCCACTCCA CAACATTCCG TTTCTGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csn2 ( NM_001286024.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272953.1 Mus musculus casein beta (Csn2), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu09155
Clone ID Related Accession (Same CDS sequence)	NM_001286024.1
Accession Version	NM_001286024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	beta-casein isoform e precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074046.9 and AK085698.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI113016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GGAATCTGTT 
GAACATATCA ATGAGAAACT TCAGAAGGTG AATCTCATGG GACAGCTGCA GGCAGAGGAT 
GTGCTCCAGG CTAAAGTTCA CTCCAGCATC CAGTCACAGC CCCAGGCCTT TCCATATGCT 
CAGGCTCAAA CCATCTCTTG CAATCCCGTC CCACAAAACA TCCAGCCTAT TGCTCAACCC 
CCTGTGGTGC CATCTCTTGG GCCTGTCATT TCTCCTGAAC TGGAATCCTT CCTTAAAGCT 
AAAGCCACCA TCCTTCCCAA GCACAAACAG ATGCCCCTCC TTAACTCTGA AACTGTGCTC 
CGCCTCATAA ACTCTCAAAT CCCCAGCCTT GCCAGTCTTG CTAATCTGCA CCTTCCTCAG 
TCTCTGGTCC AGCTCCTGGC ACAGGTTGTT CAGGCTTTTC CTCAGACTCA CCTGGTTTCT 
TCTCAGACCC AGCTGTCTCT TCCTCAGTCC AAAGTTCTGT ACTTTCTGCA GCAAGTAGCA 
CCCTTCCTTC CACAAGATAT GTCTGTCCAA GACCTTCTGC AGTACCTAGA ACTTCTTAAC 
CCCACCGTCC AATTCCCTGC CACTCCACAA CATTCCGTTT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272953.1
CDS	60..707
Translation	MKVFILACLVALALAREESVEHINEKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAFPQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQHSVSV

Target ORF information:

RefSeq Version	NM_001286024.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus casein beta (Csn2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286024.1

ORF Insert Sequence:

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GGAATCTGTT 
GAACATATCA ATGAGAAACT TCAGAAGGTG AATCTCATGG GACAGCTGCA GGCAGAGGAT 
GTGCTCCAGG CTAAAGTTCA CTCCAGCATC CAGTCACAGC CCCAGGCCTT TCCATATGCT 
CAGGCTCAAA CCATCTCTTG CAATCCCGTC CCACAAAACA TCCAGCCTAT TGCTCAACCC 
CCTGTGGTGC CATCTCTTGG GCCTGTCATT TCTCCTGAAC TGGAATCCTT CCTTAAAGCT 
AAAGCCACCA TCCTTCCCAA GCACAAACAG ATGCCCCTCC TTAACTCTGA AACTGTGCTC 
CGCCTCATAA ACTCTCAAAT CCCCAGCCTT GCCAGTCTTG CTAATCTGCA CCTTCCTCAG 
TCTCTGGTCC AGCTCCTGGC ACAGGTTGTT CAGGCTTTTC CTCAGACTCA CCTGGTTTCT 
TCTCAGACCC AGCTGTCTCT TCCTCAGTCC AAAGTTCTGT ACTTTCTGCA GCAAGTAGCA 
CCCTTCCTTC CACAAGATAT GTCTGTCCAA GACCTTCTGC AGTACCTAGA ACTTCTTAAC 
CCCACCGTCC AATTCCCTGC CACTCCACAA CATTCCGTTT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csn2 ( NM_001286020.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272949.1 Mus musculus casein beta (Csn2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09150
Clone ID Related Accession (Same CDS sequence)	NM_001286020.1 , NM_001286021.1
Accession Version	NM_001286020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	beta-casein isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074046.9 and AK085698.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Both variants 2 and 3 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK021324.1, AK021328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTACATTT 
ACTGTATCCT CTGAGACTGA TAGTATTTCC AGTGAGGAAT CTGTTGAACA TATCAATGAG 
AAACTTCAGA AGGTGAATCT CATGGGACAG CTGCAGGCAG AGGATGTGCT CCAGGCTAAA 
GTTCACTCCA GCATCCAGTC ACAGCCCCAG GCCTTTCCAT ATGCTCAGGC TCAAACCATC 
TCTTGCAATC CCGTCCCACA AAACATCCAG CCTATTGCTC AACCCCCTGT GGTGCCATCT 
CTTGGGCCTG TCATTTCTCC TGAACTGGAA TCCTTCCTTA AAGCTAAAGC CACCATCCTT 
CCCAAGCACA AACAGATGCC CCTCCTTAAC TCTGAAACTG TGCTCCGCCT CATAAACTCT 
CAAATCCCCA GCCTTGCCAG TCTTGCTAAT CTGCACCTTC CTCAGTCTCT GGTCCAGCTC 
CTGGCACAGG TTGTTCAGGC TTTTCCTCAG ACTCACCTGG TTTCTTCTCA GACCCAGCTG 
TCTCTTCCTC AGTCCAAAGT TCTGTACTTT CTGCAGCAAG TAGCACCCTT CCTTCCACAA 
GATATGTCTG TCCAAGACCT TCTGCAGTAC CTAGAACTTC TTAACCCCAC CGTCCAATTC 
CCTGCCACTC CACAACATTC CGTTTCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272949.1
CDS	60..752
Translation	MKVFILACLVALALARETTFTVSSETDSISSEESVEHINEKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAFPQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQHSVSV

Target ORF information:

RefSeq Version	NM_001286020.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus casein beta (Csn2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286020.1

ORF Insert Sequence:

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTACATTT 
ACTGTATCCT CTGAGACTGA TAGTATTTCC AGTGAGGAAT CTGTTGAACA TATCAATGAG 
AAACTTCAGA AGGTGAATCT CATGGGACAG CTGCAGGCAG AGGATGTGCT CCAGGCTAAA 
GTTCACTCCA GCATCCAGTC ACAGCCCCAG GCCTTTCCAT ATGCTCAGGC TCAAACCATC 
TCTTGCAATC CCGTCCCACA AAACATCCAG CCTATTGCTC AACCCCCTGT GGTGCCATCT 
CTTGGGCCTG TCATTTCTCC TGAACTGGAA TCCTTCCTTA AAGCTAAAGC CACCATCCTT 
CCCAAGCACA AACAGATGCC CCTCCTTAAC TCTGAAACTG TGCTCCGCCT CATAAACTCT 
CAAATCCCCA GCCTTGCCAG TCTTGCTAAT CTGCACCTTC CTCAGTCTCT GGTCCAGCTC 
CTGGCACAGG TTGTTCAGGC TTTTCCTCAG ACTCACCTGG TTTCTTCTCA GACCCAGCTG 
TCTCTTCCTC AGTCCAAAGT TCTGTACTTT CTGCAGCAAG TAGCACCCTT CCTTCCACAA 
GATATGTCTG TCCAAGACCT TCTGCAGTAC CTAGAACTTC TTAACCCCAC CGTCCAATTC 
CCTGCCACTC CACAACATTC CGTTTCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csn2 ( NM_001286021.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272950.1 Mus musculus casein beta (Csn2), transcript variant 3, mRNA.

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CloneID	OMu09150
Clone ID Related Accession (Same CDS sequence)	NM_001286020.1 , NM_001286021.1
Accession Version	NM_001286021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	beta-casein isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074046.9 and AK085698.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Both variants 2 and 3 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI110598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTACATTT 
ACTGTATCCT CTGAGACTGA TAGTATTTCC AGTGAGGAAT CTGTTGAACA TATCAATGAG 
AAACTTCAGA AGGTGAATCT CATGGGACAG CTGCAGGCAG AGGATGTGCT CCAGGCTAAA 
GTTCACTCCA GCATCCAGTC ACAGCCCCAG GCCTTTCCAT ATGCTCAGGC TCAAACCATC 
TCTTGCAATC CCGTCCCACA AAACATCCAG CCTATTGCTC AACCCCCTGT GGTGCCATCT 
CTTGGGCCTG TCATTTCTCC TGAACTGGAA TCCTTCCTTA AAGCTAAAGC CACCATCCTT 
CCCAAGCACA AACAGATGCC CCTCCTTAAC TCTGAAACTG TGCTCCGCCT CATAAACTCT 
CAAATCCCCA GCCTTGCCAG TCTTGCTAAT CTGCACCTTC CTCAGTCTCT GGTCCAGCTC 
CTGGCACAGG TTGTTCAGGC TTTTCCTCAG ACTCACCTGG TTTCTTCTCA GACCCAGCTG 
TCTCTTCCTC AGTCCAAAGT TCTGTACTTT CTGCAGCAAG TAGCACCCTT CCTTCCACAA 
GATATGTCTG TCCAAGACCT TCTGCAGTAC CTAGAACTTC TTAACCCCAC CGTCCAATTC 
CCTGCCACTC CACAACATTC CGTTTCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272950.1
CDS	75..767
Translation	MKVFILACLVALALARETTFTVSSETDSISSEESVEHINEKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAFPQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQHSVSV

Target ORF information:

RefSeq Version	NM_001286021.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus casein beta (Csn2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286021.1

ORF Insert Sequence:

ATGAAGGTCT TCATCCTCGC CTGCCTTGTG GCCCTTGCTC TTGCAAGAGA GACTACATTT 
ACTGTATCCT CTGAGACTGA TAGTATTTCC AGTGAGGAAT CTGTTGAACA TATCAATGAG 
AAACTTCAGA AGGTGAATCT CATGGGACAG CTGCAGGCAG AGGATGTGCT CCAGGCTAAA 
GTTCACTCCA GCATCCAGTC ACAGCCCCAG GCCTTTCCAT ATGCTCAGGC TCAAACCATC 
TCTTGCAATC CCGTCCCACA AAACATCCAG CCTATTGCTC AACCCCCTGT GGTGCCATCT 
CTTGGGCCTG TCATTTCTCC TGAACTGGAA TCCTTCCTTA AAGCTAAAGC CACCATCCTT 
CCCAAGCACA AACAGATGCC CCTCCTTAAC TCTGAAACTG TGCTCCGCCT CATAAACTCT 
CAAATCCCCA GCCTTGCCAG TCTTGCTAAT CTGCACCTTC CTCAGTCTCT GGTCCAGCTC 
CTGGCACAGG TTGTTCAGGC TTTTCCTCAG ACTCACCTGG TTTCTTCTCA GACCCAGCTG 
TCTCTTCCTC AGTCCAAAGT TCTGTACTTT CTGCAGCAAG TAGCACCCTT CCTTCCACAA 
GATATGTCTG TCCAAGACCT TCTGCAGTAC CTAGAACTTC TTAACCCCAC CGTCCAATTC 
CCTGCCACTC CACAACATTC CGTTTCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in ?-casein secretion pathway in lactating mammary epithelial cells.
Molecular and cellular endocrinology44016-24(2017 Jan)
Kobayashi K,Oyama S,Kuki C,Tsugami Y,Matsunaga K,Suzuki T,Nishimura T

Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with ?-casein expression in mammary epithelial cells.
Biochimica et biophysica acta1863(8)2006-16(2016 08)
Kobayashi K,Tsugami Y,Matsunaga K,Oyama S,Kuki C,Kumura H

Interactions of the ubiquitous octamer-binding transcription factor-1 with both the signal transducer and activator of transcription 5 and the glucocorticoid receptor mediate prolactin and glucocorticoid-induced ?-casein gene expression in mammary epithelial cells.
The international journal of biochemistry & cell biology45(3)724-35(2013 Mar)
Qian X,Zhao FQ

The Stat6-regulated KRAB domain zinc finger protein Zfp157 regulates the balance of lineages in mammary glands and compensates for loss of Gata-3.
Genes & development26(10)1086-97(2012 May)
Oliver CH,Khaled WT,Frend H,Nichols J,Watson CJ

Insulin-like growth factors 1 and 2 regulate expression of ?-casein in vitro in mouse mammary epithelial cells.
Bulletin of experimental biology and medicine152(2)202-5(2011 Dec)
Shushanov SS