Ccs cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccs
Entrez Gene ID	12460
Full Name	copper chaperone for superoxide dismutase
Synonyms	Ccsd
General protein information	Preferred Names copper chaperone for superoxide dismutase Names copper chaperone for superoxide dismutase superoxide dismutase copper chaperone
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 4.11 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_016892.3	NP_058588.1	copper chaperone for superoxide dismutase
XM_006531645.2	XP_006531708.1	copper chaperone for superoxide dismutase isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ccs	NP_445877.1
Danio rerio (zebrafish)	ccs	NP_001191151.1
	CCS	XP_001118002.2
Canis lupus familiaris (dog)	CCS	NP_001181899.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007026	XP_308747.4
Homo sapiens (human)	CCS	NP_005116.1
Rattus norvegicus (Norway rat)	LOC100911589	XP_003749080.1
Drosophila melanogaster (fruit fly)	CCS	NP_001163108.1
Saccharomyces cerevisiae (baker's yeast)	CCS1	NP_013752.1
Magnaporthe oryzae (rice blast fungus)	MGG_05281	XP_003712840.1
Arabidopsis thaliana (thale cress)	CCS	NP_563910.2
Oryza sativa (rice)	Os04g0573200	NP_001053613.1
Mus musculus (house mouse)	Ccs	NP_058588.1
Neurospora crassa	NCU07851	XP_963112.2
Xenopus tropicalis (tropical clawed frog)	ccs	NP_001011020.1
Pan troglodytes (chimpanzee)	CCS	NP_001167001.1
Kluyveromyces lactis	KLLA0F26917g	XP_456277.1

Gene Ontology
Bibliography

Related articles in PubMed

The mitochondrial metallochaperone SCO1 maintains CTR1 at the plasma membrane to preserve copper homeostasis in the murine heart.
Baker ZN, Jett K, Boulet A, Hossain A, Cobine PA, Kim BE, El Zawily AM, Lee L, Tibbits GF, Petris MJ, Leary SC
Human molecular genetics26(23)4617-4628(2017 Dec)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

Effects of Cu,Zn-superoxide dismutase on cell proliferation and neuroblast differentiation in the mouse dentate gyrus.
Yoo DY, Shin BN, Kim IH, Kim W, Kim DW, Yoo KY, Choi JH, Lee CH, Yoon YS, Choi SY, Won MH, Hwang IK
Neurochemical research37(2)261-7(2012 Feb)

Erythrocyte copper chaperone for superoxide dismutase is increased following marginal copper deficiency in adult and postweanling mice.
Lassi KC, Prohaska JR
The Journal of nutrition142(2)292-7(2012 Feb)

Roles of copper chaperone for superoxide dismutase 1 and metallothionein in copper homeostasis.
Miyayama T, Ishizuka Y, Iijima T, Hiraoka D, Ogra Y
Metallomics : integrated biometal science3(7)693-701(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that a SOD1 supplement to healthy mice may not be necessary to modulate cell proliferation and neuroblast differentiation in the dentate gyrus
Title: Effects of Cu,Zn-superoxide dismutase on cell proliferation and neuroblast differentiation in the mouse dentate gyrus.

Small interfering RNA (siRNA) targeting CCS was introduced into metallothionein-knockout mouse fibroblasts (MT-KO cells) and their wild type cells (MT-WT cells) to reveal the interactive role of CCS with other Cu-regulating proteins.
Title: Roles of copper chaperone for superoxide dismutase 1 and metallothionein in copper homeostasis.

effects of copper chaperone for SOD1 on mitochondrial dysfunction are SOD1 mutation dependent and correlate with SOD1 redox susceptibility
Title: Redox susceptibility of SOD1 mutants is associated with the differential response to CCS over-expression in vivo.

Study finds that as expected, CCS over-expression promotes oxidation of a wild-type SOD1 disulfide; however, in the diseased G93A/CCS mouse, there was no increased oxidation of the mutant SOD1 disulfide.
Title: Biological effects of CCS in the absence of SOD1 enzyme activation: implications for disease in a mouse model for ALS.

These results establish that CCS/G93A SOD1 mice manifest an isolated complex IV deficiency which may underlie a substantial part of mutant SOD1-induced mitochondrial cytopathy.
Title: Isolated cytochrome c oxidase deficiency in G93A SOD1 mice overexpressing CCS protein.

The following Ccs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu55008	XM_006531645.2 Latest version!	Mus musculus copper chaperone for superoxide dismutase (Ccs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu10525	NM_016892.3 Latest version!	Mus musculus copper chaperone for superoxide dismutase (Ccs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccs ( XM_006531645.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531708.1 Mus musculus copper chaperone for superoxide dismutase (Ccs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55008
Clone ID Related Accession (Same CDS sequence)	XM_006531645.2
Accession Version	XM_006531645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	copper chaperone for superoxide dismutase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGA AGTCGGGGGA CGGTGGGACT GTGTGTGCGT TGGAGTTTGC AGTGCAGATG 
AGTTGTCAGA GCTGTGTGGA TGCTGTGCAC AAGACCCTGA AAGGGGTGGC AGGTGTCCAG 
AATGTGGACG TTCAGTTGGA GAACCAGATG GTGTTGGTGC AGACGACTCT GCCCAGCCAG 
GAGGTACAAG CACTCCTGGA AAGCACAGGG AGGCAGGCTG TACTCAAGGG TATGGGCAGT 
AGCCAATTAC AGAATCTGGG AGCAGCAGTG GCCATTCTGG AGGGCTGTGG CTCCATACAG 
GGGGTGGTCC GCTTCCTACA GCTATCCTCT GAACTCTGCT TGATTGAGGG AACCATCGAC 
GGCCTAGAGC CTGGGCTGCA TGGACTCCAT GTGCATCAGT ATGGGGACCT TACGAGGGAC 
TGCAATAGCA CCGGGGAGAT CTGGGCAATG TCCGGGCTGA AGCTGGTGGC CGAGCTACCT 
TCCGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531708.1
CDS	93..581
Translation	MASKSGDGGTVCALEFAVQMSCQSCVDAVHKTLKGVAGVQNVDVQLENQMVLVQTTLPSQEVQALLESTGRQAVLKGMGSSQLQNLGAAVAILEGCGSIQGVVRFLQLSSELCLIEGTIDGLEPGLHGLHVHQYGDLTRDCNSTGEIWAMSGLKLVAELPSG

Target ORF information:

RefSeq Version	XM_006531645.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus copper chaperone for superoxide dismutase (Ccs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531645.2

ORF Insert Sequence:

ATGGCTTCGA AGTCGGGGGA CGGTGGGACT GTGTGTGCGT TGGAGTTTGC AGTGCAGATG 
AGTTGTCAGA GCTGTGTGGA TGCTGTGCAC AAGACCCTGA AAGGGGTGGC AGGTGTCCAG 
AATGTGGACG TTCAGTTGGA GAACCAGATG GTGTTGGTGC AGACGACTCT GCCCAGCCAG 
GAGGTACAAG CACTCCTGGA AAGCACAGGG AGGCAGGCTG TACTCAAGGG TATGGGCAGT 
AGCCAATTAC AGAATCTGGG AGCAGCAGTG GCCATTCTGG AGGGCTGTGG CTCCATACAG 
GGGGTGGTCC GCTTCCTACA GCTATCCTCT GAACTCTGCT TGATTGAGGG AACCATCGAC 
GGCCTAGAGC CTGGGCTGCA TGGACTCCAT GTGCATCAGT ATGGGGACCT TACGAGGGAC 
TGCAATAGCA CCGGGGAGAT CTGGGCAATG TCCGGGCTGA AGCTGGTGGC CGAGCTACCT 
TCCGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccs ( NM_016892.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058588.1 Mus musculus copper chaperone for superoxide dismutase (Ccs), mRNA.

Price & Availability↑

CloneID	OMu10525
Clone ID Related Accession (Same CDS sequence)	NM_016892.3
Accession Version	NM_016892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	copper chaperone for superoxide dismutase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY073478.1, AK050072.1 and CF929049.1. On Sep 14, 2006 this sequence version replaced NM_016892.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF173379.1, BC026938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCGA AGTCGGGGGA CGGTGGGACT GTGTGTGCGT TGGAGTTTGC AGTGCAGATG 
AGTTGTCAGA GCTGTGTGGA TGCTGTGCAC AAGACCCTGA AAGGGGTGGC AGGTGTCCAG 
AATGTGGACG TTCAGTTGGA GAACCAGATG GTGTTGGTGC AGACGACTCT GCCCAGCCAG 
GAGGTACAAG CACTCCTGGA AAGCACAGGG AGGCAGGCTG TACTCAAGGG TATGGGCAGT 
AGCCAATTAC AGAATCTGGG AGCAGCAGTG GCCATTCTGG AGGGCTGTGG CTCCATACAG 
GGGGTGGTCC GCTTCCTACA GCTATCCTCT GAACTCTGCT TGATTGAGGG AACCATCGAC 
GGCCTAGAGC CTGGGCTGCA TGGACTCCAT GTGCATCAGT ATGGGGACCT TACGAGGGAC 
TGCAATAGCT GTGGGGACCA TTTTAACCCT GATGGAGCAT CTCATGGGGG TCCTCAGGAC 
ACTGATCGGC ACCGGGGAGA TCTGGGCAAT GTCCGGGCTG AAGCTGGTGG CCGAGCTACC 
TTCCGGATAG AGGATAAACA GCTGAAGGTG TGGGATGTGA TTGGCCGCAG CCTGGTTATC 
GATGAGGGGG AAGATGACCT GGGCCGGGGA GGCCATCCCT TATCCAAGAT CACAGGGAAT 
TCTGGGAAGA GGTTGGCCTG TGGCATCATT GCACGCTCTG CCGGCCTTTT CCAGAACCCC 
AAGCAGATCT GCTCCTGTGA TGGCCTCACT ATCTGGGAGG AGCGAGGCCG GCCCATTGCC 
GGTCAAGGCC GAAAGGACTC AGCCCAACCC CCAGCTCACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058588.1
CDS	88..912
Translation	MASKSGDGGTVCALEFAVQMSCQSCVDAVHKTLKGVAGVQNVDVQLENQMVLVQTTLPSQEVQALLESTGRQAVLKGMGSSQLQNLGAAVAILEGCGSIQGVVRFLQLSSELCLIEGTIDGLEPGLHGLHVHQYGDLTRDCNSCGDHFNPDGASHGGPQDTDRHRGDLGNVRAEAGGRATFRIEDKQLKVWDVIGRSLVIDEGEDDLGRGGHPLSKITGNSGKRLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGQGRKDSAQPPAHL

Target ORF information:

RefSeq Version	NM_016892.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus copper chaperone for superoxide dismutase (Ccs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016892.3

ORF Insert Sequence:

ATGGCTTCGA AGTCGGGGGA CGGTGGGACT GTGTGTGCGT TGGAGTTTGC AGTGCAGATG 
AGTTGTCAGA GCTGTGTGGA TGCTGTGCAC AAGACCCTGA AAGGGGTGGC AGGTGTCCAG 
AATGTGGACG TTCAGTTGGA GAACCAGATG GTGTTGGTGC AGACGACTCT GCCCAGCCAG 
GAGGTACAAG CACTCCTGGA AAGCACAGGG AGGCAGGCTG TACTCAAGGG TATGGGCAGT 
AGCCAATTAC AGAATCTGGG AGCAGCAGTG GCCATTCTGG AGGGCTGTGG CTCCATACAG 
GGGGTGGTCC GCTTCCTACA GCTATCCTCT GAACTCTGCT TGATTGAGGG AACCATCGAC 
GGCCTAGAGC CTGGGCTGCA TGGACTCCAT GTGCATCAGT ATGGGGACCT TACGAGGGAC 
TGCAATAGCT GTGGGGACCA TTTTAACCCT GATGGAGCAT CTCATGGGGG TCCTCAGGAC 
ACTGATCGGC ACCGGGGAGA TCTGGGCAAT GTCCGGGCTG AAGCTGGTGG CCGAGCTACC 
TTCCGGATAG AGGATAAACA GCTGAAGGTG TGGGATGTGA TTGGCCGCAG CCTGGTTATC 
GATGAGGGGG AAGATGACCT GGGCCGGGGA GGCCATCCCT TATCCAAGAT CACAGGGAAT 
TCTGGGAAGA GGTTGGCCTG TGGCATCATT GCACGCTCTG CCGGCCTTTT CCAGAACCCC 
AAGCAGATCT GCTCCTGTGA TGGCCTCACT ATCTGGGAGG AGCGAGGCCG GCCCATTGCC 
GGTCAAGGCC GAAAGGACTC AGCCCAACCC CCAGCTCACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mitochondrial metallochaperone SCO1 maintains CTR1 at the plasma membrane to preserve copper homeostasis in the murine heart.
Human molecular genetics26(23)4617-4628(2017 Dec)
Baker ZN,Jett K,Boulet A,Hossain A,Cobine PA,Kim BE,El Zawily AM,Lee L,Tibbits GF,Petris MJ,Leary SC

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

Effects of Cu,Zn-superoxide dismutase on cell proliferation and neuroblast differentiation in the mouse dentate gyrus.
Neurochemical research37(2)261-7(2012 Feb)
Yoo DY,Shin BN,Kim IH,Kim W,Kim DW,Yoo KY,Choi JH,Lee CH,Yoon YS,Choi SY,Won MH,Hwang IK

Erythrocyte copper chaperone for superoxide dismutase is increased following marginal copper deficiency in adult and postweanling mice.
The Journal of nutrition142(2)292-7(2012 Feb)
Lassi KC,Prohaska JR

Roles of copper chaperone for superoxide dismutase 1 and metallothionein in copper homeostasis.
Metallomics : integrated biometal science3(7)693-701(2011 Jul)
Miyayama T,Ishizuka Y,Iijima T,Hiraoka D,Ogra Y