Thoc5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thoc5
Entrez Gene ID	107829
Full Name	THO complex 5
Synonyms	1700060C24Rik,A430085L24Rik,Fmip,PK1.3,Sgk2
General protein information	Preferred Names THO complex 5 Names THO complex subunit 5 homolog Fms interacting protein fms-interacting protein serum/glucocorticoid regulated kinase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006514448.2	XP_006514511.2	THO complex subunit 5 homolog isoform X1
NM_172438.3	NP_766026.1	THO complex subunit 5 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THOC5	XP_001135376.2
Canis lupus familiaris (dog)	THOC5	XP_534728.2
Danio rerio (zebrafish)	thoc5	NP_997857.1
Homo sapiens (human)	THOC5	NP_001002877.1
Bos taurus (cattle)	THOC5	NP_001077185.1
Mus musculus (house mouse)	Thoc5	NP_766026.1
Rattus norvegicus (Norway rat)	Thoc5	NP_001012153.1
Gallus gallus (chicken)	THOC5	NP_001006195.1
Drosophila melanogaster (fruit fly)	thoc5	NP_611856.1
	LOC714842	XP_002808014.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004746	XP_001689094.1
Xenopus tropicalis (tropical clawed frog)	thoc5	NP_001016827.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of Modifier Genes in a Mouse Model of Gaucher Disease.
Klein AD, Ferreira NS, Ben-Dor S, Duan J, Hardy J, Cox TM, Merrill AH Jr, Futerman AH
Cell reports16(10)2546-2553(2016 09)

Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts.
Sakuma T, Tonne JM, Ikeda Y
Viruses6(3)1135-48(2014 Mar)

The THO complex regulates pluripotency gene mRNA export and controls embryonic stem cell self-renewal and somatic cell reprogramming.
Wang L, Miao YL, Zheng X, Lackford B, Zhou B, Han L, Yao C, Ward JM, Burkholder A, Lipchina I, Fargo DC, Hochedlinger K, Shi Y, Williams CJ, Hu G
Cell stem cell13(6)676-90(2013 Dec)

THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier.
Saran S, Tran DD, Klebba-F?rber S, Moran-Losada P, Wiehlmann L, Koch A, Chopra H, Pabst O, Hoffmann A, Klopfleisch R, Tamura T
BMC cell biology1451(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results imply that murine leukemia virus requires UAP56, THOC5 and THOC7, in addition to NXF1, for nuclear export of viral transcripts.
Title: Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts.

THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier.
Title: THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier.

Regulation of immediate-early gene response by THOC5, a member of mRNA export complex contributes to the M-CSF-induced macrophage differentiation.
Title: Transcriptional regulation of immediate-early gene response by THOC5, a member of mRNA export complex, contributes to the M-CSF-induced macrophage differentiation.

THOC5 may be required for mRNA export under stress and/or upon signaling-induced conditions
Title: Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts.

THOC5/Fms interacting protein is an essential element in the maintenance of hematopoiesis, and depletion of THOC5/Fms interacting protein causes the down-regulation of THOC1, which may contribute to altered THO complex function and cell death
Title: THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo.

The following Thoc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thoc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16091	NM_172438.3 Latest version!	Mus musculus THO complex 5 (Thoc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu72191	XM_006514448.2 Latest version!	Mus musculus THO complex 5 (Thoc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thoc5 ( NM_172438.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766026.1 Mus musculus THO complex 5 (Thoc5), mRNA.

Price & Availability↑

CloneID	OMu16091
Clone ID Related Accession (Same CDS sequence)	NM_172438.3
Accession Version	NM_172438.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Mus musculus(house mouse)
Product	THO complex subunit 5 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL645522.12. On May 1, 2007 this sequence version replaced NM_172438.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK050734.1, BC039758.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCATCAG AATCCAGCAA AAAACGGAAA CCCAAAGTGA TCCGGAGTGA TGGAACCCCA 
ACTGAAGGGA AACGGAACCG GTCTGACACG GAGCAGGAAG GCAAATACTA CAGTGAGGAG 
GCTGAAGTTG ACCTGCGGGA CCCTGGCAGA GACTATGAGT TGTACAAATA CACTTGCCAG 
GAGCTACAAA GGCTGATGGC CGAGATCCAG GACCTGAAGA GTAAGGGCAG CAAGGACCTG 
GCCATTGAAA TTGAAGAACG AAGGATCCAG AGCTGTGTGC ACTTCATGAC TCTAAAGAAG 
CTTAACCGAT TAGCCCACAT CAGATTAAAG AAAGGAAGAG ACCAGACCCA CGAGGCCAAG 
CAGAAAGTCG ATGCCTACCA CCTACAGCTA CAGAACCTGC TCTACGAAGT GATGCACCTG 
CAGAAGGAGA TCACCAAATG TCTGGAGTTC AAGTCAAAGC ATGAAGAAAT TGATCTGGTC 
AGCTTAGAGG AATTTTATTC AGAGGCTCCA CCAAGCATCA GCAAGGCCGA GATCACCATG 
GGAGACCCTC ACCAACAAAC ATTGGCACGT TTGGACTGGG AGCTGGAGCA GCGGAAAAGG 
CTGGCAGAAA AGTACCGAGA ATGTCTGTCT AACAAGGAGA AGATCCTGAA GGAGATTGAG 
GTGAAGAGGG ACTATCTGAG CAGCCTCCAG CCTCGCCTCA ACAGCATCAT GCAGGCTTCC 
CTGCCAGTGC AAGAATACCT GTTCATGCCA TTTGACCAGG CGCACAAACA GTATGAGACT 
GCCAGGCACC TGCCGCCTCC CCTCTATGTG CTCTTTGTGC AGGCCACTGC ATACGGGCAG 
GCCTGTGATA AGACGCTGTC CGTGGCAATT GAAGGCAGTG TGGACGAAGC CAAAGCTCTT 
TTCAAGCCCC CTGAGGACTC CCAAGATGAT GAGAGCGACT CAGATGCTGA AGAGGAGCAG 
ACGACGAAGC GGCGGCGGCC CACTCTCGGG GTTCAGTTGG ATGACAAGCG CAAGGAGATG 
CTAAAAAGGC ACCCACTGTC TGTCCTGCTG GATCTGAAGT GCAAAGACAA CAGTGTGCTT 
CATCTGACTT TCTACTATCT CATGAACCTC AACATCATGA CAGTGAAAGC CAAAGTGACG 
ACCGCCGTGG AGCTGATCAC CCCCATCAGT GCAGGAGACT TACTGTCTCC TGACTCGGTC 
TTGAGCTGCT TGTATCCGGG AGATCATGGG AAAAAAACTC CAAATCCAGC CAATCAGTAT 
CAGTTTGACA AAGTTGGCAT CCTGACTTTG AGAGACTATG TACTTGAGTT AGGTCACCCC 
TACTTGTGGG TACAGAAGCT GGGTGGCCTC CACTTCCCCA AAGAGCAGCC CCAGCAAACA 
GTGATGCCTG ACCACTCACA GAGCGCCAGC CACATGGAGA CCACCATGAA ACTGCTGAAG 
ACCAGGGTAC AGTCCCGCCT GGCTCTCCAC AAACAGTTTG CATCTCTGGA ACATGGCATT 
GTGCCCGTCA CCAGCGATTG CCAGGACCTC TTCCCTGCCA AGGTTGTTTC TCGCCTGGTA 
AAGTGGGTGA TAATTACCCA TGAAGATTAC ATGGAGCTGC ACTTCACTAA GGACATTGTG 
GAGGCAGGAC TGGCCGGAGA CACCAATCTC TACTACCTGG CGCTAATTGA AAGGGGAACA 
GCTAAACTTC AGGCTGCTGT GGTACTAAAC CCTGGCTACT CCTCCATCCC ACCGGTTTTC 
CGACTCTGTC TAAACTGGAA AGGGGAGAAA ACCAACAGCA ACGATGACAA TATTCGGGCT 
ATGGAGAGTG AAGTCAACGT GTGCTATAAG GAGTTGTGTG GCCCTCGGCC CAGTCACCAG 
CTCCTGACCA ACCAGCTGCA GAGACTGTGT GTGCTGCTGG ACGTCTACCT GGAGACCGAG 
AGCCACGATG ACAGCTTCGA GGGCCCCAAG GAGTTTCCCC AGGAGAAGAT GTGCCTGCGG 
CTTTTTAGGG GTCCGAGTAG GATGAAGCCG TTTAAATACA ACCATCCCCA AGGGTTCTTC 
AGCCACCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766026.1
CDS	57..2108
Translation	MSSESSKKRKPKVIRSDGTPTEGKRNRSDTEQEGKYYSEEAEVDLRDPGRDYELYKYTCQELQRLMAEIQDLKSKGSKDLAIEIEERRIQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAKQKVDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHEEIDLVSLEEFYSEAPPSISKAEITMGDPHQQTLARLDWELEQRKRLAEKYRECLSNKEKILKEIEVKRDYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAIEGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVLLDLKCKDNSVLHLTFYYLMNLNIMTVKAKVTTAVELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLRDYVLELGHPYLWVQKLGGLHFPKEQPQQTVMPDHSQSASHMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQDLFPAKVVSRLVKWVIITHEDYMELHFTKDIVEAGLAGDTNLYYLALIERGTAKLQAAVVLNPGYSSIPPVFRLCLNWKGEKTNSNDDNIRAMESEVNVCYKELCGPRPSHQLLTNQLQRLCVLLDVYLETESHDDSFEGPKEFPQEKMCLRLFRGPSRMKPFKYNHPQGFFSHR

Target ORF information:

RefSeq Version	NM_172438.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus THO complex 5 (Thoc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172438.3

ORF Insert Sequence:

ATGTCATCAG AATCCAGCAA AAAACGGAAA CCCAAAGTGA TCCGGAGTGA TGGAACCCCA 
ACTGAAGGGA AACGGAACCG GTCTGACACG GAGCAGGAAG GCAAATACTA CAGTGAGGAG 
GCTGAAGTTG ACCTGCGGGA CCCTGGCAGA GACTATGAGT TGTACAAATA CACTTGCCAG 
GAGCTACAAA GGCTGATGGC CGAGATCCAG GACCTGAAGA GTAAGGGCAG CAAGGACCTG 
GCCATTGAAA TTGAAGAACG AAGGATCCAG AGCTGTGTGC ACTTCATGAC TCTAAAGAAG 
CTTAACCGAT TAGCCCACAT CAGATTAAAG AAAGGAAGAG ACCAGACCCA CGAGGCCAAG 
CAGAAAGTCG ATGCCTACCA CCTACAGCTA CAGAACCTGC TCTACGAAGT GATGCACCTG 
CAGAAGGAGA TCACCAAATG TCTGGAGTTC AAGTCAAAGC ATGAAGAAAT TGATCTGGTC 
AGCTTAGAGG AATTTTATTC AGAGGCTCCA CCAAGCATCA GCAAGGCCGA GATCACCATG 
GGAGACCCTC ACCAACAAAC ATTGGCACGT TTGGACTGGG AGCTGGAGCA GCGGAAAAGG 
CTGGCAGAAA AGTACCGAGA ATGTCTGTCT AACAAGGAGA AGATCCTGAA GGAGATTGAG 
GTGAAGAGGG ACTATCTGAG CAGCCTCCAG CCTCGCCTCA ACAGCATCAT GCAGGCTTCC 
CTGCCAGTGC AAGAATACCT GTTCATGCCA TTTGACCAGG CGCACAAACA GTATGAGACT 
GCCAGGCACC TGCCGCCTCC CCTCTATGTG CTCTTTGTGC AGGCCACTGC ATACGGGCAG 
GCCTGTGATA AGACGCTGTC CGTGGCAATT GAAGGCAGTG TGGACGAAGC CAAAGCTCTT 
TTCAAGCCCC CTGAGGACTC CCAAGATGAT GAGAGCGACT CAGATGCTGA AGAGGAGCAG 
ACGACGAAGC GGCGGCGGCC CACTCTCGGG GTTCAGTTGG ATGACAAGCG CAAGGAGATG 
CTAAAAAGGC ACCCACTGTC TGTCCTGCTG GATCTGAAGT GCAAAGACAA CAGTGTGCTT 
CATCTGACTT TCTACTATCT CATGAACCTC AACATCATGA CAGTGAAAGC CAAAGTGACG 
ACCGCCGTGG AGCTGATCAC CCCCATCAGT GCAGGAGACT TACTGTCTCC TGACTCGGTC 
TTGAGCTGCT TGTATCCGGG AGATCATGGG AAAAAAACTC CAAATCCAGC CAATCAGTAT 
CAGTTTGACA AAGTTGGCAT CCTGACTTTG AGAGACTATG TACTTGAGTT AGGTCACCCC 
TACTTGTGGG TACAGAAGCT GGGTGGCCTC CACTTCCCCA AAGAGCAGCC CCAGCAAACA 
GTGATGCCTG ACCACTCACA GAGCGCCAGC CACATGGAGA CCACCATGAA ACTGCTGAAG 
ACCAGGGTAC AGTCCCGCCT GGCTCTCCAC AAACAGTTTG CATCTCTGGA ACATGGCATT 
GTGCCCGTCA CCAGCGATTG CCAGGACCTC TTCCCTGCCA AGGTTGTTTC TCGCCTGGTA 
AAGTGGGTGA TAATTACCCA TGAAGATTAC ATGGAGCTGC ACTTCACTAA GGACATTGTG 
GAGGCAGGAC TGGCCGGAGA CACCAATCTC TACTACCTGG CGCTAATTGA AAGGGGAACA 
GCTAAACTTC AGGCTGCTGT GGTACTAAAC CCTGGCTACT CCTCCATCCC ACCGGTTTTC 
CGACTCTGTC TAAACTGGAA AGGGGAGAAA ACCAACAGCA ACGATGACAA TATTCGGGCT 
ATGGAGAGTG AAGTCAACGT GTGCTATAAG GAGTTGTGTG GCCCTCGGCC CAGTCACCAG 
CTCCTGACCA ACCAGCTGCA GAGACTGTGT GTGCTGCTGG ACGTCTACCT GGAGACCGAG 
AGCCACGATG ACAGCTTCGA GGGCCCCAAG GAGTTTCCCC AGGAGAAGAT GTGCCTGCGG 
CTTTTTAGGG GTCCGAGTAG GATGAAGCCG TTTAAATACA ACCATCCCCA AGGGTTCTTC 
AGCCACCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thoc5 ( XM_006514448.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514511.2 Mus musculus THO complex 5 (Thoc5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72191
Clone ID Related Accession (Same CDS sequence)	XM_006514448.2
Accession Version	XM_006514448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	THO complex subunit 5 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006514448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAG AATCCAGCAA AAAACGGAAA CCCAAAGTGA TCCGGAGTGA TGGAACCCCA 
ACTGAAGGGA AACGGAACCG GTCTGACACG GAGCAGGAAG GCAAATACTA CAGTGAGGAG 
GCTGAAGTTG ACCTGCGGGA CCCTGGCAGA GACTATGAGT TGTACAAATA CACTTGCCAG 
GAGCTACAAA GGCTGATGGC CGAGATCCAG GACCTGAAGA GTAAGGGCAG CAAGGACCTG 
GCCATTGAAA TTGAAGAACG AAGGATCCAG AGCTGTGTGC ACTTCATGAC TCTAAAGAAG 
CTTAACCGAT TAGCCCACAT CAGATTAAAG AAAGGAAGAG ACCAGACCCA CGAGGCCAAG 
CAGAAAGTCG ATGCCTACCA CCTACAGCTA CAGAACCTGC TCTACGAAGT GATGCACCTG 
CAGAAGGAGA TCACCAAATG TCTGGAGTTC AAGTCAAAGC ATGAAGAAAT TGATCTGGTC 
AGCTTAGAGG AATTTTATTC AGAGGCTCCA CCAAGCATCA GCAAGGCCGA GATCACCATG 
GGAGACCCTC ACCAACAAAC ATTGGCACGT TTGGACTGGG AGCTGGAGCA GCGGAAAAGG 
CTGGCAGAAA AGTACCGAGA ATGTCTGTCT AACAAGGAGA AGATCCTGAA GGAGATTGAG 
GTGAAGAGGG ACTATCTGAG CAGCCTCCAG CCTCGCCTCA ACAGCATCAT GCAGGCTTCC 
CTGCCAGTGC AAGAATACCT GTTCATGCCA TTTGACCAGG CGCACAAACA GTATGAGACT 
GCCAGGCACC TGCCGCCTCC CCTCTATGTG CTCTTTGTGC AGGCCACTGC ATACGGGCAG 
GCCTGTGCTC ATATGAAATC CTCCTCCCAG CCCCCTAGAC AGGATAAGAC GCTGTCCGTG 
GCAATTGAAG GCAGTGTGGA CGAAGCCAAA GCTCTTTTCA AGCCCCCTGA GGACTCCCAA 
GATGATGAGA GCGACTCAGA TGCTGAAGAG GAGCAGACGA CGAAGCGGCG GCGGCCCACT 
CTCGGGGTTC AGTTGGATGA CAAGCGCAAG GAGATGCTAA AAAGGCACCC ACTGTCTGTC 
CTGCTGGATC TGAAGTGCAA AGACAACAGT GTGCTTCATC TGACTTTCTA CTATCTCATG 
AACCTCAACA TCATGACAGT GAAAGCCAAA GTGACGACCG CCGTGGAGCT GATCACCCCC 
ATCAGTGCAG GAGACTTACT GTCTCCTGAC TCGGTCTTGA GCTGCTTGTA TCCGGGAGAT 
CATGGGAAAA AAACTCCAAA TCCAGCCAAT CAGTATCAGT TTGACAAAGT TGGCATCCTG 
ACTTTGAGAG ACTATGTACT TGAGTTAGGT CACCCCTACT TGTGGGTACA GAAGCTGGGT 
GGCCTCCACT TCCCCAAAGA GCAGCCCCAG CAAACAGTGA TGCCTGACCA CTCACAGAGC 
GCCAGCCACA TGGAGACCAC CATGAAACTG CTGAAGACCA GGGTACAGTC CCGCCTGGCT 
CTCCACAAAC AGTTTGCATC TCTGGAACAT GGCATTGTGC CCGTCACCAG CGATTGCCAG 
GACCTCTTCC CTGCCAAGGT TGTTTCTCGC CTGGTAAAGT GGGTGATAAT TACCCATGAA 
GATTACATGG AGCTGCACTT CACTAAGGAC ATTGTGGAGG CAGGACTGGC CGGAGACACC 
AATCTCTACT ACCTGGCGCT AATTGAAAGG GGAACAGCTA AACTTCAGGC TGCTGTGGTA 
CTAAACCCTG GCTACTCCTC CATCCCACCG GTTTTCCGAC TCTGTCTAAA CTGGAAAGGG 
GAGAAAACCA ACAGCAACGA TGACAATATT CGGGCTATGG AGAGTGAAGT CAACGTGTGC 
TATAAGGAGT TGTGTGGCCC TCGGCCCAGT CACCAGCTCC TGACCAACCA GCTGCAGAGA 
CTGTGTGTGC TGCTGGACGT CTACCTGGAG ACCGAGAGCC ACGATGACAG CTTCGAGGGC 
CCCAAGGAGT TTCCCCAGGA GAAGATGTGC CTGCGGCTTT TTAGGGGTCC GAGTAGGATG 
AAGCCGTTTA AATACAACCA TCCCCAAGGG TTCTTCAGCC ACCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514511.2
CDS	117..2204
Translation	MSSESSKKRKPKVIRSDGTPTEGKRNRSDTEQEGKYYSEEAEVDLRDPGRDYELYKYTCQELQRLMAEIQDLKSKGSKDLAIEIEERRIQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAKQKVDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHEEIDLVSLEEFYSEAPPSISKAEITMGDPHQQTLARLDWELEQRKRLAEKYRECLSNKEKILKEIEVKRDYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACAHMKSSSQPPRQDKTLSVAIEGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVLLDLKCKDNSVLHLTFYYLMNLNIMTVKAKVTTAVELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLRDYVLELGHPYLWVQKLGGLHFPKEQPQQTVMPDHSQSASHMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQDLFPAKVVSRLVKWVIITHEDYMELHFTKDIVEAGLAGDTNLYYLALIERGTAKLQAAVVLNPGYSSIPPVFRLCLNWKGEKTNSNDDNIRAMESEVNVCYKELCGPRPSHQLLTNQLQRLCVLLDVYLETESHDDSFEGPKEFPQEKMCLRLFRGPSRMKPFKYNHPQGFFSHR

Target ORF information:

RefSeq Version	XM_006514448.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus THO complex 5 (Thoc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514448.2

ORF Insert Sequence:

ATGTCATCAG AATCCAGCAA AAAACGGAAA CCCAAAGTGA TCCGGAGTGA TGGAACCCCA 
ACTGAAGGGA AACGGAACCG GTCTGACACG GAGCAGGAAG GCAAATACTA CAGTGAGGAG 
GCTGAAGTTG ACCTGCGGGA CCCTGGCAGA GACTATGAGT TGTACAAATA CACTTGCCAG 
GAGCTACAAA GGCTGATGGC CGAGATCCAG GACCTGAAGA GTAAGGGCAG CAAGGACCTG 
GCCATTGAAA TTGAAGAACG AAGGATCCAG AGCTGTGTGC ACTTCATGAC TCTAAAGAAG 
CTTAACCGAT TAGCCCACAT CAGATTAAAG AAAGGAAGAG ACCAGACCCA CGAGGCCAAG 
CAGAAAGTCG ATGCCTACCA CCTACAGCTA CAGAACCTGC TCTACGAAGT GATGCACCTG 
CAGAAGGAGA TCACCAAATG TCTGGAGTTC AAGTCAAAGC ATGAAGAAAT TGATCTGGTC 
AGCTTAGAGG AATTTTATTC AGAGGCTCCA CCAAGCATCA GCAAGGCCGA GATCACCATG 
GGAGACCCTC ACCAACAAAC ATTGGCACGT TTGGACTGGG AGCTGGAGCA GCGGAAAAGG 
CTGGCAGAAA AGTACCGAGA ATGTCTGTCT AACAAGGAGA AGATCCTGAA GGAGATTGAG 
GTGAAGAGGG ACTATCTGAG CAGCCTCCAG CCTCGCCTCA ACAGCATCAT GCAGGCTTCC 
CTGCCAGTGC AAGAATACCT GTTCATGCCA TTTGACCAGG CGCACAAACA GTATGAGACT 
GCCAGGCACC TGCCGCCTCC CCTCTATGTG CTCTTTGTGC AGGCCACTGC ATACGGGCAG 
GCCTGTGCTC ATATGAAATC CTCCTCCCAG CCCCCTAGAC AGGATAAGAC GCTGTCCGTG 
GCAATTGAAG GCAGTGTGGA CGAAGCCAAA GCTCTTTTCA AGCCCCCTGA GGACTCCCAA 
GATGATGAGA GCGACTCAGA TGCTGAAGAG GAGCAGACGA CGAAGCGGCG GCGGCCCACT 
CTCGGGGTTC AGTTGGATGA CAAGCGCAAG GAGATGCTAA AAAGGCACCC ACTGTCTGTC 
CTGCTGGATC TGAAGTGCAA AGACAACAGT GTGCTTCATC TGACTTTCTA CTATCTCATG 
AACCTCAACA TCATGACAGT GAAAGCCAAA GTGACGACCG CCGTGGAGCT GATCACCCCC 
ATCAGTGCAG GAGACTTACT GTCTCCTGAC TCGGTCTTGA GCTGCTTGTA TCCGGGAGAT 
CATGGGAAAA AAACTCCAAA TCCAGCCAAT CAGTATCAGT TTGACAAAGT TGGCATCCTG 
ACTTTGAGAG ACTATGTACT TGAGTTAGGT CACCCCTACT TGTGGGTACA GAAGCTGGGT 
GGCCTCCACT TCCCCAAAGA GCAGCCCCAG CAAACAGTGA TGCCTGACCA CTCACAGAGC 
GCCAGCCACA TGGAGACCAC CATGAAACTG CTGAAGACCA GGGTACAGTC CCGCCTGGCT 
CTCCACAAAC AGTTTGCATC TCTGGAACAT GGCATTGTGC CCGTCACCAG CGATTGCCAG 
GACCTCTTCC CTGCCAAGGT TGTTTCTCGC CTGGTAAAGT GGGTGATAAT TACCCATGAA 
GATTACATGG AGCTGCACTT CACTAAGGAC ATTGTGGAGG CAGGACTGGC CGGAGACACC 
AATCTCTACT ACCTGGCGCT AATTGAAAGG GGAACAGCTA AACTTCAGGC TGCTGTGGTA 
CTAAACCCTG GCTACTCCTC CATCCCACCG GTTTTCCGAC TCTGTCTAAA CTGGAAAGGG 
GAGAAAACCA ACAGCAACGA TGACAATATT CGGGCTATGG AGAGTGAAGT CAACGTGTGC 
TATAAGGAGT TGTGTGGCCC TCGGCCCAGT CACCAGCTCC TGACCAACCA GCTGCAGAGA 
CTGTGTGTGC TGCTGGACGT CTACCTGGAG ACCGAGAGCC ACGATGACAG CTTCGAGGGC 
CCCAAGGAGT TTCCCCAGGA GAAGATGTGC CTGCGGCTTT TTAGGGGTCC GAGTAGGATG 
AAGCCGTTTA AATACAACCA TCCCCAAGGG TTCTTCAGCC ACCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of Modifier Genes in a Mouse Model of Gaucher Disease.
Cell reports16(10)2546-2553(2016 09)
Klein AD,Ferreira NS,Ben-Dor S,Duan J,Hardy J,Cox TM,Merrill AH Jr,Futerman AH

Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts.
Viruses6(3)1135-48(2014 Mar)
Sakuma T,Tonne JM,Ikeda Y

The THO complex regulates pluripotency gene mRNA export and controls embryonic stem cell self-renewal and somatic cell reprogramming.
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THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier.
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