Nlrp3 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrp3
Entrez Gene ID	110305596
Full Name	NLR family pyrin domain containing 3
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021177615.1	XP_021033274.1	NACHT, LRR and PYD domains-containing protein 3
XM_021177615.2	XP_021033274.1	NACHT, LRR and PYD domains-containing protein 3

Pathways from BioSystems
Gene Ontology

The following Nlrp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv27567	XM_021177615.2 Latest version!	Mus caroli NLR family pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OMv27567	XM_021177615.1	Mus caroli NLR family pyrin domain containing 3 (Nlrp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrp3 ( XM_021177615.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021033274.1 Mus caroli NLR family pyrin domain containing 3 (Nlrp3), mRNA.

Price & Availability↑

CloneID	OMv27567
Clone ID Related Accession (Same CDS sequence)	XM_021177615.1 , XM_021177615.2
Accession Version	XM_021177615.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG TCCGTTGCAA GCTGGCCCAG TATCTAGAGG ACCTTGAAGA TATAGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CCGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACGCGTGTAC ATCTCATTCC 
TCTATGGTAT GCCAGGAGGA CAGCATTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAAAAGAAA GATTACTGTA AGATGTACAG AAGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TGGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CGAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TATTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCGCAC GGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCGGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCTGTGT GCAAGATCCT GCGCAAGTCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCACG TGGAGATCCT AGGCTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCCGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CTGGGAAGAG CCTAGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGCCATTG AGGAGCATCT CTTCTCTGAC 
TACCTGCAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGTGGAA GCACGGCCTG CAGAAGACTG ACGTCTCTGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGTGACC 
GTGAGGAAAG GGCCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTATG GGAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCCTATTTG GAGAAGAAAC TAAGCTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAGCTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGCG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CATGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGG 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAATTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAGTCAGAT TGCTGTGTGT GGGACTGAAG 
CATCTGCTCT GCAACCTCCA GAAACTCTGG TTGGTGAGCT GCTGTCTCAC ATCCGAGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAG GTTTTGTGTG AAAAGATGAA GGACCCACAA 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTACCTACG AAACAACGCC 
CTTGGAGACA CGGGACTCAG GCTTCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GTAACAACGA TTTGGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAACAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC ACTCCAGGAA 
GAAAAGCCTG AGCTGACTAT TGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021033274.1
CDS	123..3224
Translation	MTSVRCKLAQYLEDLEDIDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDACTSHSSMVCQEDSIEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVRTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKSSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGAIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLWKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGVTVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGVRLLCVGLKHLLCNLQKLWLVSCCLTSECCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRNNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	XM_021177615.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli NLR family pyrin domain containing 3 (Nlrp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021177615.1

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCCCAG TATCTAGAGG ACCTTGAAGA TATAGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CCGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACGCGTGTAC ATCTCATTCC 
TCTATGGTAT GCCAGGAGGA CAGCATTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAAAAGAAA GATTACTGTA AGATGTACAG AAGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TGGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CGAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TATTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCGCAC GGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCGGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCTGTGT GCAAGATCCT GCGCAAGTCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCACG TGGAGATCCT AGGCTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCCGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CTGGGAAGAG CCTAGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGCCATTG AGGAGCATCT CTTCTCTGAC 
TACCTGCAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGTGGAA GCACGGCCTG CAGAAGACTG ACGTCTCTGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGTGACC 
GTGAGGAAAG GGCCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTATG GGAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCCTATTTG GAGAAGAAAC TAAGCTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAGCTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGCG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CATGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGG 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAATTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAGTCAGAT TGCTGTGTGT GGGACTGAAG 
CATCTGCTCT GCAACCTCCA GAAACTCTGG TTGGTGAGCT GCTGTCTCAC ATCCGAGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAG GTTTTGTGTG AAAAGATGAA GGACCCACAA 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTACCTACG AAACAACGCC 
CTTGGAGACA CGGGACTCAG GCTTCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GTAACAACGA TTTGGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAACAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC ACTCCAGGAA 
GAAAAGCCTG AGCTGACTAT TGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( XM_021177615.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021033274.1 Mus caroli NLR family pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv27567
Clone ID Related Accession (Same CDS sequence)	XM_021177615.1 , XM_021177615.2
Accession Version	XM_021177615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021177615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG TCCGTTGCAA GCTGGCCCAG TATCTAGAGG ACCTTGAAGA TATAGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CCGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACGCGTGTAC ATCTCATTCC 
TCTATGGTAT GCCAGGAGGA CAGCATTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAAAAGAAA GATTACTGTA AGATGTACAG AAGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TGGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CGAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TATTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCGCAC GGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCGGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCTGTGT GCAAGATCCT GCGCAAGTCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCACG TGGAGATCCT AGGCTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCCGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CTGGGAAGAG CCTAGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGCCATTG AGGAGCATCT CTTCTCTGAC 
TACCTGCAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGTGGAA GCACGGCCTG CAGAAGACTG ACGTCTCTGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGTGACC 
GTGAGGAAAG GGCCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTATG GGAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCCTATTTG GAGAAGAAAC TAAGCTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAGCTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGCG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CATGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGG 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAATTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAGTCAGAT TGCTGTGTGT GGGACTGAAG 
CATCTGCTCT GCAACCTCCA GAAACTCTGG TTGGTGAGCT GCTGTCTCAC ATCCGAGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAG GTTTTGTGTG AAAAGATGAA GGACCCACAA 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTACCTACG AAACAACGCC 
CTTGGAGACA CGGGACTCAG GCTTCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GTAACAACGA TTTGGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAACAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC ACTCCAGGAA 
GAAAAGCCTG AGCTGACTAT TGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021033274.1
CDS	139..3240
Translation	MTSVRCKLAQYLEDLEDIDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDACTSHSSMVCQEDSIEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVRTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKSSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGAIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLWKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGVTVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGVRLLCVGLKHLLCNLQKLWLVSCCLTSECCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRNNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	XM_021177615.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli NLR family pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021177615.2

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCCCAG TATCTAGAGG ACCTTGAAGA TATAGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CCGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACGCGTGTAC ATCTCATTCC 
TCTATGGTAT GCCAGGAGGA CAGCATTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAAAAGAAA GATTACTGTA AGATGTACAG AAGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TGGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CGAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TATTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCGCAC GGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCGGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCTGTGT GCAAGATCCT GCGCAAGTCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCACG TGGAGATCCT AGGCTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCCGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CTGGGAAGAG CCTAGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGCCATTG AGGAGCATCT CTTCTCTGAC 
TACCTGCAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGTGGAA GCACGGCCTG CAGAAGACTG ACGTCTCTGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGTGACC 
GTGAGGAAAG GGCCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTATG GGAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCCTATTTG GAGAAGAAAC TAAGCTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAGCTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGCG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CATGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGG 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAATTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAGTCAGAT TGCTGTGTGT GGGACTGAAG 
CATCTGCTCT GCAACCTCCA GAAACTCTGG TTGGTGAGCT GCTGTCTCAC ATCCGAGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAG GTTTTGTGTG AAAAGATGAA GGACCCACAA 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTACCTACG AAACAACGCC 
CTTGGAGACA CGGGACTCAG GCTTCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GTAACAACGA TTTGGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAACAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC ACTCCAGGAA 
GAAAAGCCTG AGCTGACTAT TGTCTTCGAG ATTTCCTGGT AG