Pitrm1 cDNA ORF clone, Microtus ochrogaster(prairie vole)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pitrm1
Entrez Gene ID	102002698
Full Name	pitrilysin metallopeptidase 1
Gene Type	protein-coding
Organism	Microtus ochrogaster(prairie vole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005365397.2	XP_005365454.1	presequence protease, mitochondrial
XM_005365397.3	XP_005365454.1	presequence protease, mitochondrial

Pathways from BioSystems
Gene Ontology

The following Pitrm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pitrm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMi119117	XM_005365397.3 Latest version!	Microtus ochrogaster pitrilysin metallopeptidase 1 (Pitrm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OMi119117	XM_005365397.2	Microtus ochrogaster pitrilysin metallopeptidase 1 (Pitrm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pitrm1 ( XM_005365397.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_005365454.1 Microtus ochrogaster pitrilysin metallopeptidase 1 (Pitrm1), mRNA.

Price & Availability↑

CloneID	OMi119117
Clone ID Related Accession (Same CDS sequence)	XM_005365397.2 , XM_005365397.3
Accession Version	XM_005365397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	presequence protease, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004949100.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2015 this sequence version replaced XM_005365397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCGCT TCAGCGGTCG TCGGGGATTG TGCGCGGTAC AGCGACTGAG CTGCGGAGCG 
CACCACAAAG TGTGGAGGGG GAGGAGTGAT CAAGCCTGTG AACGAGCTCT GCAGTATAAA 
GTGGGCGAAA AGATCCACGG CTTCACCGTC AAGCAGGTCA CTCCTGTCCC TGAACTGTTC 
CTGACAGCGG TAAAGCTCAG CCATGACTGT ACGGGAGCCA GATACCTGCA CCTGGCGAGG 
GAGGACAAAA ACAACTTATT CAGTGTCCAG TTCCGGACGA CCCCGATGGA CAGCACTGGA 
GTCCCCCATG TTCTTGAGCA TACAGTCCTC TGTGGTTCTC AGAAGTATCC ATGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCATTG TCCACATTTA TGAATGCCTT CACAGCTAGC 
GACTATACGA TGTATCCGTT CTCCACACAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCA 
GTGTATCTGG ATGCGACTTT CTTCCCATGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AACACGAGAA TCCAAGTGAC CCTCAGTCTC CCTTGATCTT TAAAGGAGTC 
GTCTTTAATG AGATGAAAGG GGCGTTTACA GACAATGAGA GGATATTCTC CCAGCACCTT 
CAGAACAAGC TTCTTCCTGA CCATACGTAC TCTGTGGTCT CTGGCGGGGA CCCACTGTGC 
ATCCCAGAGC TCACGTGGGA GCAGCTGAAA GAGTTCCATG CGACTCACTA TCACCCAAGC 
AATGCCAGGT TCTTCACGTA CGGCAACTTT CCACTGGAAC AGCACCTGAA GCAAATTCAT 
GAAGAGGCCC TGAGTAAATT TCAGAAAATG GAGCCTAGTA CAGCCGTGCC GGCCCAGCAG 
CACTGGGACA AGCCTAGAGA ATTCCATATA ACATGCGGCC CAGACTCGTT AGCTACGGAT 
GCCTCTAAGC AAACTACTGT CAGCGTTAGC TTCCTCTTAC CAGACATCAC TGACACCTTT 
GAAGCCTTCA CGCTGAACCT TCTGTCTTCC CTCCTGATTT CTGGGCCTAA TTCCCCCTTT 
TACAAAGCTT TGATTGAATC TGGACTCGGC ACAGACTTTT CCCCTGATGT TGGATACAAT 
GGCTACACGA GGGAGGCTTA CTTCAATGTT GGCCTCCAAG GGATTGCTGA GAAAGATGTC 
AGGACCGTCA GAGAGATCAT AGACAGGACA ATCGAAGAAG TTATTGAGCA AGGATTTGAA 
GATGACCGGA TTGAAGCTTT ACTTCATAAA ATCGAAATCC AGATGAAGCA TCAGTCTACC 
AGCTTTGGCC TGTCGCTGAC GTCATATATA GCCTCTTGCT GGAATCATGA CGGGGACCCC 
GTGGAGCTCC TGCAGATGGG AAATCAGTTG ACTAAATTTA GGCAGTGCCT TAAAGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAGCAG TATTTTAAGA ACAATCAGCA CAAGATGACT 
TTATCCATGA AACCGGATGA CAAATATTAT GAGAAACAAA CGCAAATGGA GACGGAAAAG 
CTAGCGCAAA AGGTGAATTC TCTCTCCCAG GCGGACAAGC AGCAGATCTA TCAGAAAGGT 
TTAGAATTAC GAACGCAGCA AAGTAAACCT CAAGACGCCT CCTGTCTCCC AGCACTGAAA 
GTCTCTGACA TTGAGCCCAT CATGCCTTTC ACCAAGTTTG ACATCGCCCT CGCAGCTGGA 
GATGTCCCTG TGCAGTACTG CCCGCAGCCC ACCAACGGCA TGGTCTATTT CCGAGCCTTT 
TCCAACTTAA ACACCCTTCC AGAGGAGTTG AGGCCCTATG TGCCTCTCTT CTGCAGTGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTGAC TACAGAGAGC AAGCCCAGCA GATTGAGCTG 
AAGACAGGAG GCATGACGGT TTCACCCCAC GTGCTCCCTG ATGACTCCCA GCTGGACACC 
TACGAGCAGG GTGTGCTGTT TTCATCTCTC TGCCTGGAAC GGAACCTGCC AGACATGATG 
CATCTGTGGA GTGAAATATT TAACAACCCG TGCTTTGAAG AAGAAGAGCA CTTCAAAGTG 
TTGGTGAAGA TGACAGCTCA GGAGCTCTCC AACGGCATTC CCGACTCGGG GCATCTCTAT 
GCATCCCTCA GAGCGAGCAA GACACTCACG CCTTCAGGGG ACTTAAAGGA GACCTTCGGT 
GGGATGGATC AGGTGAGGCT GATGAAAAGA ATTGCAGAAA TGACAGACAT CAAGCCCATC 
CTGAGAAAGC TACCCCGGAT CAAGAAATAC CTACTAAACT GTGATAACAT GAGATGCTCA 
GTCAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAGAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GCAGCAAAAA GGAGCGGAAA CCTGTTCGCC CACATATTGT CGAGAAACCT 
ACACACAGTG GCTCCCGGGG CAGTGCGCAT GTTAGCGGTT CCCAGATCAT CAGAAAGCTG 
ATAACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACGC ATTTCGTGCT GCCCTTCCCT 
GTGAACTATG TTGGCGAGTG TGTCAGAACC GTCCCCTATG CTGACCCAGA TCATGCCAGT 
CTTAAGATCC TTGCCCGTTT AATGACAGCG AAATTCCTGC ATACAGAAAT CCGAGAAAAG 
GGGGGTGCCT ATGGTGGAGG TGCTAAACTC AGCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CAAACTCCAT AGAAACACTC CAGTCTTTTG GGAAGGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACCCA GCAGGACATT GATGAAGCCA AGCTTTCTGT TTTCTCCGCT 
GTGGATTCTC CTGTTGCCCC ATCGGCTAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCG 
GATGAGATGA AGCAGGCTTA CCGGGAACAG CTCTTTGCTG TCAGCCATGA CAAACTGACC 
TCTGTGAGCC ATAAGTACCT TGGCATCGGG AAGAGCACAC ACAGCCTGGC TATACTCGGA 
CCAGAGAACC CAAAAATTGC CAAAGACCCA TCATGGATCG TAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005365454.1
CDS	17..3124
Translation	MWRFSGRRGLCAVQRLSCGAHHKVWRGRSDQACERALQYKVGEKIHGFTVKQVTPVPELFLTAVKLSHDCTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAFTASDYTMYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPSDPQSPLIFKGVVFNEMKGAFTDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKEFHATHYHPSNARFFTYGNFPLEQHLKQIHEEALSKFQKMEPSTAVPAQQHWDKPREFHITCGPDSLATDASKQTTVSVSFLLPDITDTFEAFTLNLLSSLLISGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFNVGLQGIAEKDVRTVREIIDRTIEEVIEQGFEDDRIEALLHKIEIQMKHQSTSFGLSLTSYIASCWNHDGDPVELLQMGNQLTKFRQCLKENPKFLQEKVEQYFKNNQHKMTLSMKPDDKYYEKQTQMETEKLAQKVNSLSQADKQQIYQKGLELRTQQSKPQDASCLPALKVSDIEPIMPFTKFDIALAAGDVPVQYCPQPTNGMVYFRAFSNLNTLPEELRPYVPLFCSVLTKLGCGILDYREQAQQIELKTGGMTVSPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFKVLVKMTAQELSNGIPDSGHLYASLRASKTLTPSGDLKETFGGMDQVRLMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTHSGSRGSAHVSGSQIIRKLITDPTFKPCQMKTHFVLPFPVNYVGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLSHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSAVDSPVAPSAKGMDHFLYGLSDEMKQAYREQLFAVSHDKLTSVSHKYLGIGKSTHSLAILGPENPKIAKDPSWIVR

Target ORF information:

RefSeq Version	XM_005365397.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster pitrilysin metallopeptidase 1 (Pitrm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005365397.2

ORF Insert Sequence:

ATGTGGCGCT TCAGCGGTCG TCGGGGATTG TGCGCGGTAC AGCGACTGAG CTGCGGAGCG 
CACCACAAAG TGTGGAGGGG GAGGAGTGAT CAAGCCTGTG AACGAGCTCT GCAGTATAAA 
GTGGGCGAAA AGATCCACGG CTTCACCGTC AAGCAGGTCA CTCCTGTCCC TGAACTGTTC 
CTGACAGCGG TAAAGCTCAG CCATGACTGT ACGGGAGCCA GATACCTGCA CCTGGCGAGG 
GAGGACAAAA ACAACTTATT CAGTGTCCAG TTCCGGACGA CCCCGATGGA CAGCACTGGA 
GTCCCCCATG TTCTTGAGCA TACAGTCCTC TGTGGTTCTC AGAAGTATCC ATGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCATTG TCCACATTTA TGAATGCCTT CACAGCTAGC 
GACTATACGA TGTATCCGTT CTCCACACAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCA 
GTGTATCTGG ATGCGACTTT CTTCCCATGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AACACGAGAA TCCAAGTGAC CCTCAGTCTC CCTTGATCTT TAAAGGAGTC 
GTCTTTAATG AGATGAAAGG GGCGTTTACA GACAATGAGA GGATATTCTC CCAGCACCTT 
CAGAACAAGC TTCTTCCTGA CCATACGTAC TCTGTGGTCT CTGGCGGGGA CCCACTGTGC 
ATCCCAGAGC TCACGTGGGA GCAGCTGAAA GAGTTCCATG CGACTCACTA TCACCCAAGC 
AATGCCAGGT TCTTCACGTA CGGCAACTTT CCACTGGAAC AGCACCTGAA GCAAATTCAT 
GAAGAGGCCC TGAGTAAATT TCAGAAAATG GAGCCTAGTA CAGCCGTGCC GGCCCAGCAG 
CACTGGGACA AGCCTAGAGA ATTCCATATA ACATGCGGCC CAGACTCGTT AGCTACGGAT 
GCCTCTAAGC AAACTACTGT CAGCGTTAGC TTCCTCTTAC CAGACATCAC TGACACCTTT 
GAAGCCTTCA CGCTGAACCT TCTGTCTTCC CTCCTGATTT CTGGGCCTAA TTCCCCCTTT 
TACAAAGCTT TGATTGAATC TGGACTCGGC ACAGACTTTT CCCCTGATGT TGGATACAAT 
GGCTACACGA GGGAGGCTTA CTTCAATGTT GGCCTCCAAG GGATTGCTGA GAAAGATGTC 
AGGACCGTCA GAGAGATCAT AGACAGGACA ATCGAAGAAG TTATTGAGCA AGGATTTGAA 
GATGACCGGA TTGAAGCTTT ACTTCATAAA ATCGAAATCC AGATGAAGCA TCAGTCTACC 
AGCTTTGGCC TGTCGCTGAC GTCATATATA GCCTCTTGCT GGAATCATGA CGGGGACCCC 
GTGGAGCTCC TGCAGATGGG AAATCAGTTG ACTAAATTTA GGCAGTGCCT TAAAGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAGCAG TATTTTAAGA ACAATCAGCA CAAGATGACT 
TTATCCATGA AACCGGATGA CAAATATTAT GAGAAACAAA CGCAAATGGA GACGGAAAAG 
CTAGCGCAAA AGGTGAATTC TCTCTCCCAG GCGGACAAGC AGCAGATCTA TCAGAAAGGT 
TTAGAATTAC GAACGCAGCA AAGTAAACCT CAAGACGCCT CCTGTCTCCC AGCACTGAAA 
GTCTCTGACA TTGAGCCCAT CATGCCTTTC ACCAAGTTTG ACATCGCCCT CGCAGCTGGA 
GATGTCCCTG TGCAGTACTG CCCGCAGCCC ACCAACGGCA TGGTCTATTT CCGAGCCTTT 
TCCAACTTAA ACACCCTTCC AGAGGAGTTG AGGCCCTATG TGCCTCTCTT CTGCAGTGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTGAC TACAGAGAGC AAGCCCAGCA GATTGAGCTG 
AAGACAGGAG GCATGACGGT TTCACCCCAC GTGCTCCCTG ATGACTCCCA GCTGGACACC 
TACGAGCAGG GTGTGCTGTT TTCATCTCTC TGCCTGGAAC GGAACCTGCC AGACATGATG 
CATCTGTGGA GTGAAATATT TAACAACCCG TGCTTTGAAG AAGAAGAGCA CTTCAAAGTG 
TTGGTGAAGA TGACAGCTCA GGAGCTCTCC AACGGCATTC CCGACTCGGG GCATCTCTAT 
GCATCCCTCA GAGCGAGCAA GACACTCACG CCTTCAGGGG ACTTAAAGGA GACCTTCGGT 
GGGATGGATC AGGTGAGGCT GATGAAAAGA ATTGCAGAAA TGACAGACAT CAAGCCCATC 
CTGAGAAAGC TACCCCGGAT CAAGAAATAC CTACTAAACT GTGATAACAT GAGATGCTCA 
GTCAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAGAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GCAGCAAAAA GGAGCGGAAA CCTGTTCGCC CACATATTGT CGAGAAACCT 
ACACACAGTG GCTCCCGGGG CAGTGCGCAT GTTAGCGGTT CCCAGATCAT CAGAAAGCTG 
ATAACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACGC ATTTCGTGCT GCCCTTCCCT 
GTGAACTATG TTGGCGAGTG TGTCAGAACC GTCCCCTATG CTGACCCAGA TCATGCCAGT 
CTTAAGATCC TTGCCCGTTT AATGACAGCG AAATTCCTGC ATACAGAAAT CCGAGAAAAG 
GGGGGTGCCT ATGGTGGAGG TGCTAAACTC AGCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CAAACTCCAT AGAAACACTC CAGTCTTTTG GGAAGGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACCCA GCAGGACATT GATGAAGCCA AGCTTTCTGT TTTCTCCGCT 
GTGGATTCTC CTGTTGCCCC ATCGGCTAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCG 
GATGAGATGA AGCAGGCTTA CCGGGAACAG CTCTTTGCTG TCAGCCATGA CAAACTGACC 
TCTGTGAGCC ATAAGTACCT TGGCATCGGG AAGAGCACAC ACAGCCTGGC TATACTCGGA 
CCAGAGAACC CAAAAATTGC CAAAGACCCA TCATGGATCG TAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitrm1 ( XM_005365397.3 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_005365454.1 Microtus ochrogaster pitrilysin metallopeptidase 1 (Pitrm1), mRNA.

Price & Availability↑

CloneID	OMi119117
Clone ID Related Accession (Same CDS sequence)	XM_005365397.2 , XM_005365397.3
Accession Version	XM_005365397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	presequence protease, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004949100.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_005365397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCGCT TCAGCGGTCG TCGGGGATTG TGCGCGGTAC AGCGACTGAG CTGCGGAGCG 
CACCACAAAG TGTGGAGGGG GAGGAGTGAT CAAGCCTGTG AACGAGCTCT GCAGTATAAA 
GTGGGCGAAA AGATCCACGG CTTCACCGTC AAGCAGGTCA CTCCTGTCCC TGAACTGTTC 
CTGACAGCGG TAAAGCTCAG CCATGACTGT ACGGGAGCCA GATACCTGCA CCTGGCGAGG 
GAGGACAAAA ACAACTTATT CAGTGTCCAG TTCCGGACGA CCCCGATGGA CAGCACTGGA 
GTCCCCCATG TTCTTGAGCA TACAGTCCTC TGTGGTTCTC AGAAGTATCC ATGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCATTG TCCACATTTA TGAATGCCTT CACAGCTAGC 
GACTATACGA TGTATCCGTT CTCCACACAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCA 
GTGTATCTGG ATGCGACTTT CTTCCCATGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AACACGAGAA TCCAAGTGAC CCTCAGTCTC CCTTGATCTT TAAAGGAGTC 
GTCTTTAATG AGATGAAAGG GGCGTTTACA GACAATGAGA GGATATTCTC CCAGCACCTT 
CAGAACAAGC TTCTTCCTGA CCATACGTAC TCTGTGGTCT CTGGCGGGGA CCCACTGTGC 
ATCCCAGAGC TCACGTGGGA GCAGCTGAAA GAGTTCCATG CGACTCACTA TCACCCAAGC 
AATGCCAGGT TCTTCACGTA CGGCAACTTT CCACTGGAAC AGCACCTGAA GCAAATTCAT 
GAAGAGGCCC TGAGTAAATT TCAGAAAATG GAGCCTAGTA CAGCCGTGCC GGCCCAGCAG 
CACTGGGACA AGCCTAGAGA ATTCCATATA ACATGCGGCC CAGACTCGTT AGCTACGGAT 
GCCTCTAAGC AAACTACTGT CAGCGTTAGC TTCCTCTTAC CAGACATCAC TGACACCTTT 
GAAGCCTTCA CGCTGAACCT TCTGTCTTCC CTCCTGATTT CTGGGCCTAA TTCCCCCTTT 
TACAAAGCTT TGATTGAATC TGGACTCGGC ACAGACTTTT CCCCTGATGT TGGATACAAT 
GGCTACACGA GGGAGGCTTA CTTCAATGTT GGCCTCCAAG GGATTGCTGA GAAAGATGTC 
AGGACCGTCA GAGAGATCAT AGACAGGACA ATCGAAGAAG TTATTGAGCA AGGATTTGAA 
GATGACCGGA TTGAAGCTTT ACTTCATAAA ATCGAAATCC AGATGAAGCA TCAGTCTACC 
AGCTTTGGCC TGTCGCTGAC GTCATATATA GCCTCTTGCT GGAATCATGA CGGGGACCCC 
GTGGAGCTCC TGCAGATGGG AAATCAGTTG ACTAAATTTA GGCAGTGCCT TAAAGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAGCAG TATTTTAAGA ACAATCAGCA CAAGATGACT 
TTATCCATGA AACCGGATGA CAAATATTAT GAGAAACAAA CGCAAATGGA GACGGAAAAG 
CTAGCGCAAA AGGTGAATTC TCTCTCCCAG GCGGACAAGC AGCAGATCTA TCAGAAAGGT 
TTAGAATTAC GAACGCAGCA AAGTAAACCT CAAGACGCCT CCTGTCTCCC AGCACTGAAA 
GTCTCTGACA TTGAGCCCAT CATGCCTTTC ACCAAGTTTG ACATCGCCCT CGCAGCTGGA 
GATGTCCCTG TGCAGTACTG CCCGCAGCCC ACCAACGGCA TGGTCTATTT CCGAGCCTTT 
TCCAACTTAA ACACCCTTCC AGAGGAGTTG AGGCCCTATG TGCCTCTCTT CTGCAGTGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTGAC TACAGAGAGC AAGCCCAGCA GATTGAGCTG 
AAGACAGGAG GCATGACGGT TTCACCCCAC GTGCTCCCTG ATGACTCCCA GCTGGACACC 
TACGAGCAGG GTGTGCTGTT TTCATCTCTC TGCCTGGAAC GGAACCTGCC AGACATGATG 
CATCTGTGGA GTGAAATATT TAACAACCCG TGCTTTGAAG AAGAAGAGCA CTTCAAAGTG 
TTGGTGAAGA TGACAGCTCA GGAGCTCTCC AACGGCATTC CCGACTCGGG GCATCTCTAT 
GCATCCCTCA GAGCGAGCAA GACACTCACG CCTTCAGGGG ACTTAAAGGA GACCTTCGGT 
GGGATGGATC AGGTGAGGCT GATGAAAAGA ATTGCAGAAA TGACAGACAT CAAGCCCATC 
CTGAGAAAGC TACCCCGGAT CAAGAAATAC CTACTAAACT GTGATAACAT GAGATGCTCA 
GTCAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAGAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GCAGCAAAAA GGAGCGGAAA CCTGTTCGCC CACATATTGT CGAGAAACCT 
ACACACAGTG GCTCCCGGGG CAGTGCGCAT GTTAGCGGTT CCCAGATCAT CAGAAAGCTG 
ATAACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACGC ATTTCGTGCT GCCCTTCCCT 
GTGAACTATG TTGGCGAGTG TGTCAGAACC GTCCCCTATG CTGACCCAGA TCATGCCAGT 
CTTAAGATCC TTGCCCGTTT AATGACAGCG AAATTCCTGC ATACAGAAAT CCGAGAAAAG 
GGGGGTGCCT ATGGTGGAGG TGCTAAACTC AGCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CAAACTCCAT AGAAACACTC CAGTCTTTTG GGAAGGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACCCA GCAGGACATT GATGAAGCCA AGCTTTCTGT TTTCTCCGCT 
GTGGATTCTC CTGTTGCCCC ATCGGCTAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCG 
GATGAGATGA AGCAGGCTTA CCGGGAACAG CTCTTTGCTG TCAGCCATGA CAAACTGACC 
TCTGTGAGCC ATAAGTACCT TGGCATCGGG AAGAGCACAC ACAGCCTGGC TATACTCGGA 
CCAGAGAACC CAAAAATTGC CAAAGACCCA TCATGGATCG TAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005365454.1
CDS	17..3124
Translation	MWRFSGRRGLCAVQRLSCGAHHKVWRGRSDQACERALQYKVGEKIHGFTVKQVTPVPELFLTAVKLSHDCTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAFTASDYTMYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPSDPQSPLIFKGVVFNEMKGAFTDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKEFHATHYHPSNARFFTYGNFPLEQHLKQIHEEALSKFQKMEPSTAVPAQQHWDKPREFHITCGPDSLATDASKQTTVSVSFLLPDITDTFEAFTLNLLSSLLISGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFNVGLQGIAEKDVRTVREIIDRTIEEVIEQGFEDDRIEALLHKIEIQMKHQSTSFGLSLTSYIASCWNHDGDPVELLQMGNQLTKFRQCLKENPKFLQEKVEQYFKNNQHKMTLSMKPDDKYYEKQTQMETEKLAQKVNSLSQADKQQIYQKGLELRTQQSKPQDASCLPALKVSDIEPIMPFTKFDIALAAGDVPVQYCPQPTNGMVYFRAFSNLNTLPEELRPYVPLFCSVLTKLGCGILDYREQAQQIELKTGGMTVSPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFKVLVKMTAQELSNGIPDSGHLYASLRASKTLTPSGDLKETFGGMDQVRLMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTHSGSRGSAHVSGSQIIRKLITDPTFKPCQMKTHFVLPFPVNYVGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLSHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSAVDSPVAPSAKGMDHFLYGLSDEMKQAYREQLFAVSHDKLTSVSHKYLGIGKSTHSLAILGPENPKIAKDPSWIVR

Target ORF information:

RefSeq Version	XM_005365397.3
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster pitrilysin metallopeptidase 1 (Pitrm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005365397.3

ORF Insert Sequence:

ATGTGGCGCT TCAGCGGTCG TCGGGGATTG TGCGCGGTAC AGCGACTGAG CTGCGGAGCG 
CACCACAAAG TGTGGAGGGG GAGGAGTGAT CAAGCCTGTG AACGAGCTCT GCAGTATAAA 
GTGGGCGAAA AGATCCACGG CTTCACCGTC AAGCAGGTCA CTCCTGTCCC TGAACTGTTC 
CTGACAGCGG TAAAGCTCAG CCATGACTGT ACGGGAGCCA GATACCTGCA CCTGGCGAGG 
GAGGACAAAA ACAACTTATT CAGTGTCCAG TTCCGGACGA CCCCGATGGA CAGCACTGGA 
GTCCCCCATG TTCTTGAGCA TACAGTCCTC TGTGGTTCTC AGAAGTATCC ATGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCATTG TCCACATTTA TGAATGCCTT CACAGCTAGC 
GACTATACGA TGTATCCGTT CTCCACACAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCA 
GTGTATCTGG ATGCGACTTT CTTCCCATGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AACACGAGAA TCCAAGTGAC CCTCAGTCTC CCTTGATCTT TAAAGGAGTC 
GTCTTTAATG AGATGAAAGG GGCGTTTACA GACAATGAGA GGATATTCTC CCAGCACCTT 
CAGAACAAGC TTCTTCCTGA CCATACGTAC TCTGTGGTCT CTGGCGGGGA CCCACTGTGC 
ATCCCAGAGC TCACGTGGGA GCAGCTGAAA GAGTTCCATG CGACTCACTA TCACCCAAGC 
AATGCCAGGT TCTTCACGTA CGGCAACTTT CCACTGGAAC AGCACCTGAA GCAAATTCAT 
GAAGAGGCCC TGAGTAAATT TCAGAAAATG GAGCCTAGTA CAGCCGTGCC GGCCCAGCAG 
CACTGGGACA AGCCTAGAGA ATTCCATATA ACATGCGGCC CAGACTCGTT AGCTACGGAT 
GCCTCTAAGC AAACTACTGT CAGCGTTAGC TTCCTCTTAC CAGACATCAC TGACACCTTT 
GAAGCCTTCA CGCTGAACCT TCTGTCTTCC CTCCTGATTT CTGGGCCTAA TTCCCCCTTT 
TACAAAGCTT TGATTGAATC TGGACTCGGC ACAGACTTTT CCCCTGATGT TGGATACAAT 
GGCTACACGA GGGAGGCTTA CTTCAATGTT GGCCTCCAAG GGATTGCTGA GAAAGATGTC 
AGGACCGTCA GAGAGATCAT AGACAGGACA ATCGAAGAAG TTATTGAGCA AGGATTTGAA 
GATGACCGGA TTGAAGCTTT ACTTCATAAA ATCGAAATCC AGATGAAGCA TCAGTCTACC 
AGCTTTGGCC TGTCGCTGAC GTCATATATA GCCTCTTGCT GGAATCATGA CGGGGACCCC 
GTGGAGCTCC TGCAGATGGG AAATCAGTTG ACTAAATTTA GGCAGTGCCT TAAAGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAGCAG TATTTTAAGA ACAATCAGCA CAAGATGACT 
TTATCCATGA AACCGGATGA CAAATATTAT GAGAAACAAA CGCAAATGGA GACGGAAAAG 
CTAGCGCAAA AGGTGAATTC TCTCTCCCAG GCGGACAAGC AGCAGATCTA TCAGAAAGGT 
TTAGAATTAC GAACGCAGCA AAGTAAACCT CAAGACGCCT CCTGTCTCCC AGCACTGAAA 
GTCTCTGACA TTGAGCCCAT CATGCCTTTC ACCAAGTTTG ACATCGCCCT CGCAGCTGGA 
GATGTCCCTG TGCAGTACTG CCCGCAGCCC ACCAACGGCA TGGTCTATTT CCGAGCCTTT 
TCCAACTTAA ACACCCTTCC AGAGGAGTTG AGGCCCTATG TGCCTCTCTT CTGCAGTGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTGAC TACAGAGAGC AAGCCCAGCA GATTGAGCTG 
AAGACAGGAG GCATGACGGT TTCACCCCAC GTGCTCCCTG ATGACTCCCA GCTGGACACC 
TACGAGCAGG GTGTGCTGTT TTCATCTCTC TGCCTGGAAC GGAACCTGCC AGACATGATG 
CATCTGTGGA GTGAAATATT TAACAACCCG TGCTTTGAAG AAGAAGAGCA CTTCAAAGTG 
TTGGTGAAGA TGACAGCTCA GGAGCTCTCC AACGGCATTC CCGACTCGGG GCATCTCTAT 
GCATCCCTCA GAGCGAGCAA GACACTCACG CCTTCAGGGG ACTTAAAGGA GACCTTCGGT 
GGGATGGATC AGGTGAGGCT GATGAAAAGA ATTGCAGAAA TGACAGACAT CAAGCCCATC 
CTGAGAAAGC TACCCCGGAT CAAGAAATAC CTACTAAACT GTGATAACAT GAGATGCTCA 
GTCAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAGAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GCAGCAAAAA GGAGCGGAAA CCTGTTCGCC CACATATTGT CGAGAAACCT 
ACACACAGTG GCTCCCGGGG CAGTGCGCAT GTTAGCGGTT CCCAGATCAT CAGAAAGCTG 
ATAACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACGC ATTTCGTGCT GCCCTTCCCT 
GTGAACTATG TTGGCGAGTG TGTCAGAACC GTCCCCTATG CTGACCCAGA TCATGCCAGT 
CTTAAGATCC TTGCCCGTTT AATGACAGCG AAATTCCTGC ATACAGAAAT CCGAGAAAAG 
GGGGGTGCCT ATGGTGGAGG TGCTAAACTC AGCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CAAACTCCAT AGAAACACTC CAGTCTTTTG GGAAGGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACCCA GCAGGACATT GATGAAGCCA AGCTTTCTGT TTTCTCCGCT 
GTGGATTCTC CTGTTGCCCC ATCGGCTAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCG 
GATGAGATGA AGCAGGCTTA CCGGGAACAG CTCTTTGCTG TCAGCCATGA CAAACTGACC 
TCTGTGAGCC ATAAGTACCT TGGCATCGGG AAGAGCACAC ACAGCCTGGC TATACTCGGA 
CCAGAGAACC CAAAAATTGC CAAAGACCCA TCATGGATCG TAAGATAA