LOC100883199 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883199
Entrez Gene ID	100883199
Full Name	probable sulfite oxidase, mitochondrial
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012286008.1	XP_012141398.1	probable sulfite oxidase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC100883199 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883199 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe97241	XM_012286008.1 Latest version!	Megachile rotundata probable sulfite oxidase, mitochondrial (LOC100883199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883199 ( XM_012286008.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141398.1 Megachile rotundata probable sulfite oxidase, mitochondrial (LOC100883199), mRNA.

Price & Availability↑

CloneID	OMe97241
Clone ID Related Accession (Same CDS sequence)	XM_012286008.1
Accession Version	XM_012286008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable sulfite oxidase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797072.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTAC GTTCGAAATT TTTGTACCAC CTAAGCAAAA AATCTTTACC ATCAGTGTCA 
AATATTTATA TTCCATGTTA TGTTGGATCA AAAAGAGAGT ATTCATTTAA AAGTGAACAA 
TTTACACAGA ACACATCTAA GAATGATTTT TATGCAAAAT GCACTGCATA CATTACCATT 
GCTACATCTC TACTGGGTAT CTATTTCTAC ACAACCCGTA AAGAAGTTTA TGCCTTGAAT 
AAAAAGGATG TTCAATGTGG AGAATTCCAA GCAAATTTGA AAACTTACAG TTTAGAGGAA 
GTAGGAAAAC ACGATAATAA AAAAGATGGT GTTTGGGTGA CATTTAAACA AGGAGTATAT 
GATGTAACAA ATTTTATTGA AAAACATCCA GGTGGTCCCT CCAAACTATT AATGGCAGCT 
GGTGGTTCGA TTGAACCATT TTGGGCAATA TTTGCAAATC ACAATAGTTC AGAAATATAT 
GAGCTTCTTG AAACAATGAG AATTGGAAAT ATTTCTGAAG AAGATACTAT TTCCAATAAA 
CTGAATGATA GTGACCCATA CTCAAATGAA CCTATTAGAC ATAAAGCTTT AAAAATTAAT 
GGACATAAAC CATTTTGTGC CGAACCACCT TCTGCTTTAT TAATTGAAAG CTTCATTACA 
CCTGTGGAAT TATTTTATGT CAGAAATCAT CTCCCTGTTC CTCACATAGA CATAAAAACT 
TATACTTTAG AACTAGCTGT GGAGGAAACA ACAAAGAAGA CCCTTAAATT TGAAGATATA 
AAAAGGTATC CCAAGTATAC AATAACAAGT GCCATAATGT GTGGTGGAAA CAGACGATCT 
GAAATGGCAA AAGAAAAAGA ATTGAAAGGG CTTAGTTGGG GTGTAGGTGC AGTGGGCAAT 
GCTACATGGA CTGGGGCAAG ACTATATGAT GTATTGAAGG ATTTGGGAAT TAAAGAAGAT 
GACTACAATC ATATACAGTT TGAGGGATAT GACTTAGATC CTTCTGGAAC ACCATATGGA 
GCAAGTATAC CTATTAGTAA AGCTATGGAT CCTAAAGCAG ATATAATTTT AGCTTATGAA 
ATGAATGGAA AACCAATATC AAAAGATCAT GGTTTTCCTA TCAGGGTAAT TGTTCCAGGT 
GTGGTGGGTG CCAGAAATGT GAAATGGCTT GCTAAAATAA TTGTCTCAAA AGAAGAAAGT 
CAGTCACAGT GGCAGCGACG TGATTATAAA GGTTTTTCTC CAAGTACTGA TTGGAATAAT 
GTAGATTTTT CTAAAGCACC TGCTATTCAA GAAATGCCTG TCGTTTCTGC AATTTGTGTA 
CCAGAATCAT CAGAAACAGT TAAAGTAGTA AATGGAAAAG TAAATGTCAA AGGATATGCA 
TGGTCTGGAG GAGGTCGAAA AATTATTCGA GTTGATGTAA CAAATGATCA CGGTAAAACT 
TGGTATACAG CTAATTTTCA TGCGGAAGAT AGCAATGCTA AAGAAGGTCG TTATTGGAGC 
TGGACATTAT GGAATGTAGA TCTTCCCGTT AATAAAGAAT CAAAAGAAAT GGAAATTTGG 
GCAAAAGCTG TAGATGCATC ATACAATGTT CAACCAGAAA GTTTTAAAAA CATTTGGAAT 
CTACGAGGTT TTCTCTGTAA TGCATATCAC AAAGTTCAAA TTAAATTAGA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141398.1
CDS	52..1728
Translation	MLLRSKFLYHLSKKSLPSVSNIYIPCYVGSKREYSFKSEQFTQNTSKNDFYAKCTAYITIATSLLGIYFYTTRKEVYALNKKDVQCGEFQANLKTYSLEEVGKHDNKKDGVWVTFKQGVYDVTNFIEKHPGGPSKLLMAAGGSIEPFWAIFANHNSSEIYELLETMRIGNISEEDTISNKLNDSDPYSNEPIRHKALKINGHKPFCAEPPSALLIESFITPVELFYVRNHLPVPHIDIKTYTLELAVEETTKKTLKFEDIKRYPKYTITSAIMCGGNRRSEMAKEKELKGLSWGVGAVGNATWTGARLYDVLKDLGIKEDDYNHIQFEGYDLDPSGTPYGASIPISKAMDPKADIILAYEMNGKPISKDHGFPIRVIVPGVVGARNVKWLAKIIVSKEESQSQWQRRDYKGFSPSTDWNNVDFSKAPAIQEMPVVSAICVPESSETVKVVNGKVNVKGYAWSGGGRKIIRVDVTNDHGKTWYTANFHAEDSNAKEGRYWSWTLWNVDLPVNKESKEMEIWAKAVDASYNVQPESFKNIWNLRGFLCNAYHKVQIKLEQ

Target ORF information:

RefSeq Version	XM_012286008.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable sulfite oxidase, mitochondrial (LOC100883199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286008.1

ORF Insert Sequence:

ATGCTTTTAC GTTCGAAATT TTTGTACCAC CTAAGCAAAA AATCTTTACC ATCAGTGTCA 
AATATTTATA TTCCATGTTA TGTTGGATCA AAAAGAGAGT ATTCATTTAA AAGTGAACAA 
TTTACACAGA ACACATCTAA GAATGATTTT TATGCAAAAT GCACTGCATA CATTACCATT 
GCTACATCTC TACTGGGTAT CTATTTCTAC ACAACCCGTA AAGAAGTTTA TGCCTTGAAT 
AAAAAGGATG TTCAATGTGG AGAATTCCAA GCAAATTTGA AAACTTACAG TTTAGAGGAA 
GTAGGAAAAC ACGATAATAA AAAAGATGGT GTTTGGGTGA CATTTAAACA AGGAGTATAT 
GATGTAACAA ATTTTATTGA AAAACATCCA GGTGGTCCCT CCAAACTATT AATGGCAGCT 
GGTGGTTCGA TTGAACCATT TTGGGCAATA TTTGCAAATC ACAATAGTTC AGAAATATAT 
GAGCTTCTTG AAACAATGAG AATTGGAAAT ATTTCTGAAG AAGATACTAT TTCCAATAAA 
CTGAATGATA GTGACCCATA CTCAAATGAA CCTATTAGAC ATAAAGCTTT AAAAATTAAT 
GGACATAAAC CATTTTGTGC CGAACCACCT TCTGCTTTAT TAATTGAAAG CTTCATTACA 
CCTGTGGAAT TATTTTATGT CAGAAATCAT CTCCCTGTTC CTCACATAGA CATAAAAACT 
TATACTTTAG AACTAGCTGT GGAGGAAACA ACAAAGAAGA CCCTTAAATT TGAAGATATA 
AAAAGGTATC CCAAGTATAC AATAACAAGT GCCATAATGT GTGGTGGAAA CAGACGATCT 
GAAATGGCAA AAGAAAAAGA ATTGAAAGGG CTTAGTTGGG GTGTAGGTGC AGTGGGCAAT 
GCTACATGGA CTGGGGCAAG ACTATATGAT GTATTGAAGG ATTTGGGAAT TAAAGAAGAT 
GACTACAATC ATATACAGTT TGAGGGATAT GACTTAGATC CTTCTGGAAC ACCATATGGA 
GCAAGTATAC CTATTAGTAA AGCTATGGAT CCTAAAGCAG ATATAATTTT AGCTTATGAA 
ATGAATGGAA AACCAATATC AAAAGATCAT GGTTTTCCTA TCAGGGTAAT TGTTCCAGGT 
GTGGTGGGTG CCAGAAATGT GAAATGGCTT GCTAAAATAA TTGTCTCAAA AGAAGAAAGT 
CAGTCACAGT GGCAGCGACG TGATTATAAA GGTTTTTCTC CAAGTACTGA TTGGAATAAT 
GTAGATTTTT CTAAAGCACC TGCTATTCAA GAAATGCCTG TCGTTTCTGC AATTTGTGTA 
CCAGAATCAT CAGAAACAGT TAAAGTAGTA AATGGAAAAG TAAATGTCAA AGGATATGCA 
TGGTCTGGAG GAGGTCGAAA AATTATTCGA GTTGATGTAA CAAATGATCA CGGTAAAACT 
TGGTATACAG CTAATTTTCA TGCGGAAGAT AGCAATGCTA AAGAAGGTCG TTATTGGAGC 
TGGACATTAT GGAATGTAGA TCTTCCCGTT AATAAAGAAT CAAAAGAAAT GGAAATTTGG 
GCAAAAGCTG TAGATGCATC ATACAATGTT CAACCAGAAA GTTTTAAAAA CATTTGGAAT 
CTACGAGGTT TTCTCTGTAA TGCATATCAC AAAGTTCAAA TTAAATTAGA GCAATGA