cfap221 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	cfap221
Entrez Gene ID	101486703
Full Name	cilia and flagella associated protein 221
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004552590.2	XP_004552647.2	cilia- and flagella-associated protein 221 isoform X2
XM_004552589.1	XP_004552646.1	cilia- and flagella-associated protein 221 isoform X1
XM_004552590.1	XP_004552647.1	cilia- and flagella-associated protein 221 isoform X2
XM_004552589.2	XP_004552646.2	cilia- and flagella-associated protein 221 isoform X1

Pathways from BioSystems
Gene Ontology

The following cfap221 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cfap221 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa23395	XM_004552589.1 Latest version!	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMa23396	XM_004552590.1 Latest version!	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMa370335	XM_004552589.2 Latest version!	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMa370336	XM_004552590.2 Latest version!	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cfap221 ( XM_004552589.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552646.1 Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa23395
Clone ID Related Accession (Same CDS sequence)	XM_004552589.1
Accession Version	XM_004552589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	cilia- and flagella-associated protein 221 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCAGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATCTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GTGAAAAGGG GAGCTGTAGG GCAAGAAGAA 
ACCTTTGCTG CTGGACCACC TAAAATGTTT TCAAGACGAG TGCTTTGTGA AGTAGGACAG 
GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT TACACCACTT TTCAGTGGGA ACTGAGACGA 
AGAGTTCTCA GACTGTTCCA GCAGGCAGCT CACAAGGTTG TGATTCGACG TCGCATGAAC 
CGGAGACTGG CTTCTTTGAA GAAACTGGCA GACAGTATGA AGAACCTGCC CTTGGCACAG 
AAAGCTGATA AAGAGAAGAC ATGCGACCTA AAAGTGTCTG CAGACAAAGT CTTTCCATCT 
TCATCCCCCT TTTTCTCTAA TGAAGATGAC CCCCTGGCTA ACATTAATTT GCCTGAACTA 
CCGGTGGATA CTCTTGATGT GACTGTGACA TCGCACATTC CTTTCTTCAA GCTACAGGTC 
CCTCTGCACT ATAAGCTTAT GGGCTACCAG GCTGTGTCAG CTTGGGAGGC ATTTAACTCT 
TATATTCCCA TGACTTTGGC TAGGCCACTG CGTACTGGAG CTCCGGACAA GCTGGAACCC 
AACAGAGCTC ATTTTCCCAT GACAGAGGAC ACCACAGCAG GAGAGCAGCA TGATGAACAA 
GACAAAGATG CAGAAACAAA GGATGATTCC AGTCTCTCTT TCTGCGCCCC TGAAAATCTG 
CTCAGACCTT TTCCAGCAAA CCCACTGAGA ATCTTTAACC CAGCTCCGGG ACTTCAGACC 
TTTAAAACAC CCCCTAAATA TTTGGAGAGT GATTTGGAGT TTCATCTCTG CCCTCTGCCC 
AGGTATACGG TCCCTGAGAG CACATTGTGT GGCATAAGGA CCCAAACGCC ACACACTCAG 
AAGAAGTTTC TCAAATGCAA GGAAGTGATC CCTGGGGTTA TGAAGTGGAA AAATTTGGAT 
TCTATCACCT TGAAAAGTCT TTCAAACCAG GCCGCACTCA CCAGTGACTG TGCTCCACGC 
AGATCTGACA ACTACAACAC AGAGATACTT CCACTCACCG CAGCTTCTCC TCTCGCCAAC 
CTCCCTGATG ATGATGTGGC CCCGCTAATG GACACACTGT GTGAAGGCTC AGGAGTCCAA 
CTGACACCCG AGATGATCAG AGCAGAATTC TTGTGTGGAG AAGTTCTCGT CTCCATTAGC 
AACCTCGACG CAGGAAAGAC AACCGTGCAC CAGAGGCAGC ATCACATTGA GGCGACCTAC 
AGGTCTGAGT TTAACCAGAT GGGAAAAAGA GTGATGACTA GACTGAAGCA ACTTGGAGTC 
ACTGACAGAA CTTCTCATTT ACCTGAAGAA GACCGTGAAA AGCCGAAGCC TGTGCACACA 
CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552646.1
CDS	166..2769
Translation	MEVALSGPQTLSEPLRRRASLPLSQLVEATQSRGNIPNHLLESKIYANLKSNSLIQAEPSELHFSGFELSKDYVKILKLINISSEVMNIHVIPTQTKYFQTTYTKKYRLIPGLAYTLKVGFCPDEWRYFYDCIRVHCKGEDNLLIPVHAYPVIDDLHIPTHIDLPAVALRQSVCHAIPLRCSCPIGFEFQVHIIQPHESFSIHPLTGVIPAKGEAKITVTFCPLQFETSQITFQLVISQFNTKPYLCTITGSCAPHIALSELEKKFSYESAVTVKHKGASPSTLVLPRSKSKYRANKEADKSKAIRDPTNVKFKLPVDVCTPAGVAKMLIKDSNKLSSKDLKQAMSSGSMIARQNKQIKEALFMKKVQQDMKEEQTNHLKWQVHLGMDPMSEHATRQILEEREIAQHEYMVKRGAVGQEETFAAGPPKMFSRRVLCEVGQAPEGVPAFQFYTTFQWELRRRVLRLFQQAAHKVVIRRRMNRRLASLKKLADSMKNLPLAQKADKEKTCDLKVSADKVFPSSSPFFSNEDDPLANINLPELPVDTLDVTVTSHIPFFKLQVPLHYKLMGYQAVSAWEAFNSYIPMTLARPLRTGAPDKLEPNRAHFPMTEDTTAGEQHDEQDKDAETKDDSSLSFCAPENLLRPFPANPLRIFNPAPGLQTFKTPPKYLESDLEFHLCPLPRYTVPESTLCGIRTQTPHTQKKFLKCKEVIPGVMKWKNLDSITLKSLSNQAALTSDCAPRRSDNYNTEILPLTAASPLANLPDDDVAPLMDTLCEGSGVQLTPEMIRAEFLCGEVLVSISNLDAGKTTVHQRQHHIEATYRSEFNQMGKRVMTRLKQLGVTDRTSHLPEEDREKPKPVHTHPIHSHE

Target ORF information:

RefSeq Version	XM_004552589.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552589.1

ORF Insert Sequence:

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCAGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATCTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GTGAAAAGGG GAGCTGTAGG GCAAGAAGAA 
ACCTTTGCTG CTGGACCACC TAAAATGTTT TCAAGACGAG TGCTTTGTGA AGTAGGACAG 
GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT TACACCACTT TTCAGTGGGA ACTGAGACGA 
AGAGTTCTCA GACTGTTCCA GCAGGCAGCT CACAAGGTTG TGATTCGACG TCGCATGAAC 
CGGAGACTGG CTTCTTTGAA GAAACTGGCA GACAGTATGA AGAACCTGCC CTTGGCACAG 
AAAGCTGATA AAGAGAAGAC ATGCGACCTA AAAGTGTCTG CAGACAAAGT CTTTCCATCT 
TCATCCCCCT TTTTCTCTAA TGAAGATGAC CCCCTGGCTA ACATTAATTT GCCTGAACTA 
CCGGTGGATA CTCTTGATGT GACTGTGACA TCGCACATTC CTTTCTTCAA GCTACAGGTC 
CCTCTGCACT ATAAGCTTAT GGGCTACCAG GCTGTGTCAG CTTGGGAGGC ATTTAACTCT 
TATATTCCCA TGACTTTGGC TAGGCCACTG CGTACTGGAG CTCCGGACAA GCTGGAACCC 
AACAGAGCTC ATTTTCCCAT GACAGAGGAC ACCACAGCAG GAGAGCAGCA TGATGAACAA 
GACAAAGATG CAGAAACAAA GGATGATTCC AGTCTCTCTT TCTGCGCCCC TGAAAATCTG 
CTCAGACCTT TTCCAGCAAA CCCACTGAGA ATCTTTAACC CAGCTCCGGG ACTTCAGACC 
TTTAAAACAC CCCCTAAATA TTTGGAGAGT GATTTGGAGT TTCATCTCTG CCCTCTGCCC 
AGGTATACGG TCCCTGAGAG CACATTGTGT GGCATAAGGA CCCAAACGCC ACACACTCAG 
AAGAAGTTTC TCAAATGCAA GGAAGTGATC CCTGGGGTTA TGAAGTGGAA AAATTTGGAT 
TCTATCACCT TGAAAAGTCT TTCAAACCAG GCCGCACTCA CCAGTGACTG TGCTCCACGC 
AGATCTGACA ACTACAACAC AGAGATACTT CCACTCACCG CAGCTTCTCC TCTCGCCAAC 
CTCCCTGATG ATGATGTGGC CCCGCTAATG GACACACTGT GTGAAGGCTC AGGAGTCCAA 
CTGACACCCG AGATGATCAG AGCAGAATTC TTGTGTGGAG AAGTTCTCGT CTCCATTAGC 
AACCTCGACG CAGGAAAGAC AACCGTGCAC CAGAGGCAGC ATCACATTGA GGCGACCTAC 
AGGTCTGAGT TTAACCAGAT GGGAAAAAGA GTGATGACTA GACTGAAGCA ACTTGGAGTC 
ACTGACAGAA CTTCTCATTT ACCTGAAGAA GACCGTGAAA AGCCGAAGCC TGTGCACACA 
CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap221 ( XM_004552590.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552647.1 Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa23396
Clone ID Related Accession (Same CDS sequence)	XM_004552590.1
Accession Version	XM_004552590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	cilia- and flagella-associated protein 221 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCAGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATCTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT 
TACACCACTT TTCAGTGGGA ACTGAGACGA AGAGTTCTCA GACTGTTCCA GCAGGCAGCT 
CACAAGGTTG TGATTCGACG TCGCATGAAC CGGAGACTGG CTTCTTTGAA GAAACTGGCA 
GACAGTATGA AGAACCTGCC CTTGGCACAG AAAGCTGATA AAGAGAAGAC ATGCGACCTA 
AAAGTGTCTG CAGACAAAGT CTTTCCATCT TCATCCCCCT TTTTCTCTAA TGAAGATGAC 
CCCCTGGCTA ACATTAATTT GCCTGAACTA CCGGTGGATA CTCTTGATGT GACTGTGACA 
TCGCACATTC CTTTCTTCAA GCTACAGGTC CCTCTGCACT ATAAGCTTAT GGGCTACCAG 
GCTGTGTCAG CTTGGGAGGC ATTTAACTCT TATATTCCCA TGACTTTGGC TAGGCCACTG 
CGTACTGGAG CTCCGGACAA GCTGGAACCC AACAGAGCTC ATTTTCCCAT GACAGAGGAC 
ACCACAGCAG GAGAGCAGCA TGATGAACAA GACAAAGATG CAGAAACAAA GGATGATTCC 
AGTCTCTCTT TCTGCGCCCC TGAAAATCTG CTCAGACCTT TTCCAGCAAA CCCACTGAGA 
ATCTTTAACC CAGCTCCGGG ACTTCAGACC TTTAAAACAC CCCCTAAATA TTTGGAGAGT 
GATTTGGAGT TTCATCTCTG CCCTCTGCCC AGGTATACGG TCCCTGAGAG CACATTGTGT 
GGCATAAGGA CCCAAACGCC ACACACTCAG AAGAAGTTTC TCAAATGCAA GGAAGTGATC 
CCTGGGGTTA TGAAGTGGAA AAATTTGGAT TCTATCACCT TGAAAAGTCT TTCAAACCAG 
GCCGCACTCA CCAGTGACTG TGCTCCACGC AGATCTGACA ACTACAACAC AGAGATACTT 
CCACTCACCG CAGCTTCTCC TCTCGCCAAC CTCCCTGATG ATGATGTGGC CCCGCTAATG 
GACACACTGT GTGAAGGCTC AGGAGTCCAA CTGACACCCG AGATGATCAG AGCAGAATTC 
TTGTGTGGAG AAGTTCTCGT CTCCATTAGC AACCTCGACG CAGGAAAGAC AACCGTGCAC 
CAGAGGCAGC ATCACATTGA GGCGACCTAC AGGTCTGAGT TTAACCAGAT GGGAAAAAGA 
GTGATGACTA GACTGAAGCA ACTTGGAGTC ACTGACAGAA CTTCTCATTT ACCTGAAGAA 
GACCGTGAAA AGCCGAAGCC TGTGCACACA CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552647.1
CDS	166..2679
Translation	MEVALSGPQTLSEPLRRRASLPLSQLVEATQSRGNIPNHLLESKIYANLKSNSLIQAEPSELHFSGFELSKDYVKILKLINISSEVMNIHVIPTQTKYFQTTYTKKYRLIPGLAYTLKVGFCPDEWRYFYDCIRVHCKGEDNLLIPVHAYPVIDDLHIPTHIDLPAVALRQSVCHAIPLRCSCPIGFEFQVHIIQPHESFSIHPLTGVIPAKGEAKITVTFCPLQFETSQITFQLVISQFNTKPYLCTITGSCAPHIALSELEKKFSYESAVTVKHKGASPSTLVLPRSKSKYRANKEADKSKAIRDPTNVKFKLPVDVCTPAGVAKMLIKDSNKLSSKDLKQAMSSGSMIARQNKQIKEALFMKKVQQDMKEEQTNHLKWQVHLGMDPMSEHATRQILEEREIAQHEYMAPEGVPAFQFYTTFQWELRRRVLRLFQQAAHKVVIRRRMNRRLASLKKLADSMKNLPLAQKADKEKTCDLKVSADKVFPSSSPFFSNEDDPLANINLPELPVDTLDVTVTSHIPFFKLQVPLHYKLMGYQAVSAWEAFNSYIPMTLARPLRTGAPDKLEPNRAHFPMTEDTTAGEQHDEQDKDAETKDDSSLSFCAPENLLRPFPANPLRIFNPAPGLQTFKTPPKYLESDLEFHLCPLPRYTVPESTLCGIRTQTPHTQKKFLKCKEVIPGVMKWKNLDSITLKSLSNQAALTSDCAPRRSDNYNTEILPLTAASPLANLPDDDVAPLMDTLCEGSGVQLTPEMIRAEFLCGEVLVSISNLDAGKTTVHQRQHHIEATYRSEFNQMGKRVMTRLKQLGVTDRTSHLPEEDREKPKPVHTHPIHSHE

Target ORF information:

RefSeq Version	XM_004552590.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552590.1

ORF Insert Sequence:

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCAGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATCTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT 
TACACCACTT TTCAGTGGGA ACTGAGACGA AGAGTTCTCA GACTGTTCCA GCAGGCAGCT 
CACAAGGTTG TGATTCGACG TCGCATGAAC CGGAGACTGG CTTCTTTGAA GAAACTGGCA 
GACAGTATGA AGAACCTGCC CTTGGCACAG AAAGCTGATA AAGAGAAGAC ATGCGACCTA 
AAAGTGTCTG CAGACAAAGT CTTTCCATCT TCATCCCCCT TTTTCTCTAA TGAAGATGAC 
CCCCTGGCTA ACATTAATTT GCCTGAACTA CCGGTGGATA CTCTTGATGT GACTGTGACA 
TCGCACATTC CTTTCTTCAA GCTACAGGTC CCTCTGCACT ATAAGCTTAT GGGCTACCAG 
GCTGTGTCAG CTTGGGAGGC ATTTAACTCT TATATTCCCA TGACTTTGGC TAGGCCACTG 
CGTACTGGAG CTCCGGACAA GCTGGAACCC AACAGAGCTC ATTTTCCCAT GACAGAGGAC 
ACCACAGCAG GAGAGCAGCA TGATGAACAA GACAAAGATG CAGAAACAAA GGATGATTCC 
AGTCTCTCTT TCTGCGCCCC TGAAAATCTG CTCAGACCTT TTCCAGCAAA CCCACTGAGA 
ATCTTTAACC CAGCTCCGGG ACTTCAGACC TTTAAAACAC CCCCTAAATA TTTGGAGAGT 
GATTTGGAGT TTCATCTCTG CCCTCTGCCC AGGTATACGG TCCCTGAGAG CACATTGTGT 
GGCATAAGGA CCCAAACGCC ACACACTCAG AAGAAGTTTC TCAAATGCAA GGAAGTGATC 
CCTGGGGTTA TGAAGTGGAA AAATTTGGAT TCTATCACCT TGAAAAGTCT TTCAAACCAG 
GCCGCACTCA CCAGTGACTG TGCTCCACGC AGATCTGACA ACTACAACAC AGAGATACTT 
CCACTCACCG CAGCTTCTCC TCTCGCCAAC CTCCCTGATG ATGATGTGGC CCCGCTAATG 
GACACACTGT GTGAAGGCTC AGGAGTCCAA CTGACACCCG AGATGATCAG AGCAGAATTC 
TTGTGTGGAG AAGTTCTCGT CTCCATTAGC AACCTCGACG CAGGAAAGAC AACCGTGCAC 
CAGAGGCAGC ATCACATTGA GGCGACCTAC AGGTCTGAGT TTAACCAGAT GGGAAAAAGA 
GTGATGACTA GACTGAAGCA ACTTGGAGTC ACTGACAGAA CTTCTCATTT ACCTGAAGAA 
GACCGTGAAA AGCCGAAGCC TGTGCACACA CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap221 ( XM_004552589.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552646.2 Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa370335
Clone ID Related Accession (Same CDS sequence)	XM_004552589.2
Accession Version	XM_004552589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	cilia- and flagella-associated protein 221 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCGGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATTTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GTGAAAAGGG GAGCTGTAGG GCAAGAAGAA 
ACCTTTGCTG CTGGACCACC TAAAATGTTT TCAAGACGAG TGCTTTGTGA AGTAGGACAG 
GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT TACACCACTT TTCAGTGGGA ACTGAGACGA 
AGAGTTCTCA GACTGTTCCA GCAGGCAGCT CACAAGGTTG TGATTCGACG TCGCATGAAC 
CGGAGACTGG CTTCTTTGAA GAAACTGGCA GACAGTATGA AGAACCTGCC CTTGGCACAG 
AAAGCTGATA AAGAGAAGAC ATGCGACCTA AAAGTGTCTG CAGACAAAGT CTTTCCATCT 
TCATCCCCCT TTTTCTCTAA TGAAGATGAC CCCCTGGCTA ACATTAATTT GCCTGAACTA 
CCGGTGGATA CTCTTGATGT GACTGTGACA TCGCACATTC CTTTCTTCAA GCTACAGGTC 
CCTCTGCACT ATAAGCTTAT GGGCTACCAG GCTGTGTCAG CTTGGGAGGC ATTTAACTCT 
TATATTCCCA TGACTTTGGC TAGGCCACTG CGTACTGGAG CTCCGGACAA GCTGGAACCC 
AACAGAGCTC ATTTTCCCAT GACAGAGGAC ACCACAGCAG GAGAGCAGCA TGATGAACAA 
GACAAAGATG CAGAAACAAA GGATGATTCC AGTCTCTCTT TCTGCGCCCC TGAAAATCTG 
CTCAGACCTT TTCCAGCAAA CCCACTGAGA ATCTTTAACC CAGCTCCGGG ACTTCAGACC 
TTTAAAACAC CCCCTAAATA TTTGGAGAGT GATTTGGAGT TTCATCTCTG CCCTCTGCCC 
AGGTATACGG TCCCTGAGAG CACATTGTGT GGCATAAGGA CCCAAACGCC ACACACTCAG 
AAGAAGTTTC TCAAATGCAA GGAAGTGATC CCTGGGGTTA TGAAGTGGAA AAATTTGGAT 
TCTATCACCT TGAAAAGTCT TTCAAACCAG GCCGCTCTCA CCAGTGACTG TGCTCCACGC 
AGATCTGACA ACTACAACAC AGAGATACTT CCACTCACCG CAGCTTCTCC TCTCGCCAAC 
CTCCCTGATG ATGATGTGGC CCCGCTAATG GACACACTGT GTGAAGGCTC AGGAGTCCAA 
CTGACACCCG AGATGATCAG AGCAGAATTC TTGTGTGGAG AAGTTCTCGT CTCCATTAGC 
AACCTCGACG CAGGAAAGAC AACCGTGCAC CAGAGGCAGC ATCACATTGA GGCGACCTAC 
AGGTCTGAGT TTAACCAGAT GGGAAAAAGA GTGATGACTA GACTGAAGCA ACTTGGAGTC 
ACTGACAGAA CTTCTCATTT ACCTGAAGAA GACCGTGAAA AGCCGAAGCC TGTGCACACA 
CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552646.2
CDS	166..2769
Translation	MEVALSGPQTLSEPLRRRASLPLSQLVEATQSRGNIPNHLLESKIYANLKSNSLIQAEPSELHFSGFELGKDYVKILKLINISSEVMNIHVIPTQTKYFQTTYTKKYRLIPGLAYTLKVGFCPDEWRYFYDCIRVHCKGEDNLLIPVHAYPVIDDLHIPTHIDLPAVALRQSVCHAIPLRCSCPIGFEFQVHIIQPHESFSIHPLTGVIPAKGEAKITVTFCPLQFETSQITFQLVISQFNTKPYLCTITGSCAPHIALSELEKKFSYESAVTVKHKGASPSTLVLPRSKSKYRANKEADKSKAIRDPTNVKFKLPVDVCTPAGVAKMLIKDSNKLSSKDLKQAMSSGSMIARQNKQIKEALFMKKVQQDMKEEQTNHLKWQVHLGMDPMSEHATRQILEEREIAQHEYMVKRGAVGQEETFAAGPPKMFSRRVLCEVGQAPEGVPAFQFYTTFQWELRRRVLRLFQQAAHKVVIRRRMNRRLASLKKLADSMKNLPLAQKADKEKTCDLKVSADKVFPSSSPFFSNEDDPLANINLPELPVDTLDVTVTSHIPFFKLQVPLHYKLMGYQAVSAWEAFNSYIPMTLARPLRTGAPDKLEPNRAHFPMTEDTTAGEQHDEQDKDAETKDDSSLSFCAPENLLRPFPANPLRIFNPAPGLQTFKTPPKYLESDLEFHLCPLPRYTVPESTLCGIRTQTPHTQKKFLKCKEVIPGVMKWKNLDSITLKSLSNQAALTSDCAPRRSDNYNTEILPLTAASPLANLPDDDVAPLMDTLCEGSGVQLTPEMIRAEFLCGEVLVSISNLDAGKTTVHQRQHHIEATYRSEFNQMGKRVMTRLKQLGVTDRTSHLPEEDREKPKPVHTHPIHSHE

Target ORF information:

RefSeq Version	XM_004552589.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552589.2

ORF Insert Sequence:

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCGGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATTTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GTGAAAAGGG GAGCTGTAGG GCAAGAAGAA 
ACCTTTGCTG CTGGACCACC TAAAATGTTT TCAAGACGAG TGCTTTGTGA AGTAGGACAG 
GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT TACACCACTT TTCAGTGGGA ACTGAGACGA 
AGAGTTCTCA GACTGTTCCA GCAGGCAGCT CACAAGGTTG TGATTCGACG TCGCATGAAC 
CGGAGACTGG CTTCTTTGAA GAAACTGGCA GACAGTATGA AGAACCTGCC CTTGGCACAG 
AAAGCTGATA AAGAGAAGAC ATGCGACCTA AAAGTGTCTG CAGACAAAGT CTTTCCATCT 
TCATCCCCCT TTTTCTCTAA TGAAGATGAC CCCCTGGCTA ACATTAATTT GCCTGAACTA 
CCGGTGGATA CTCTTGATGT GACTGTGACA TCGCACATTC CTTTCTTCAA GCTACAGGTC 
CCTCTGCACT ATAAGCTTAT GGGCTACCAG GCTGTGTCAG CTTGGGAGGC ATTTAACTCT 
TATATTCCCA TGACTTTGGC TAGGCCACTG CGTACTGGAG CTCCGGACAA GCTGGAACCC 
AACAGAGCTC ATTTTCCCAT GACAGAGGAC ACCACAGCAG GAGAGCAGCA TGATGAACAA 
GACAAAGATG CAGAAACAAA GGATGATTCC AGTCTCTCTT TCTGCGCCCC TGAAAATCTG 
CTCAGACCTT TTCCAGCAAA CCCACTGAGA ATCTTTAACC CAGCTCCGGG ACTTCAGACC 
TTTAAAACAC CCCCTAAATA TTTGGAGAGT GATTTGGAGT TTCATCTCTG CCCTCTGCCC 
AGGTATACGG TCCCTGAGAG CACATTGTGT GGCATAAGGA CCCAAACGCC ACACACTCAG 
AAGAAGTTTC TCAAATGCAA GGAAGTGATC CCTGGGGTTA TGAAGTGGAA AAATTTGGAT 
TCTATCACCT TGAAAAGTCT TTCAAACCAG GCCGCTCTCA CCAGTGACTG TGCTCCACGC 
AGATCTGACA ACTACAACAC AGAGATACTT CCACTCACCG CAGCTTCTCC TCTCGCCAAC 
CTCCCTGATG ATGATGTGGC CCCGCTAATG GACACACTGT GTGAAGGCTC AGGAGTCCAA 
CTGACACCCG AGATGATCAG AGCAGAATTC TTGTGTGGAG AAGTTCTCGT CTCCATTAGC 
AACCTCGACG CAGGAAAGAC AACCGTGCAC CAGAGGCAGC ATCACATTGA GGCGACCTAC 
AGGTCTGAGT TTAACCAGAT GGGAAAAAGA GTGATGACTA GACTGAAGCA ACTTGGAGTC 
ACTGACAGAA CTTCTCATTT ACCTGAAGAA GACCGTGAAA AGCCGAAGCC TGTGCACACA 
CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap221 ( XM_004552590.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552647.2 Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa370336
Clone ID Related Accession (Same CDS sequence)	XM_004552590.2
Accession Version	XM_004552590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	cilia- and flagella-associated protein 221 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCGGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATTTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT 
TACACCACTT TTCAGTGGGA ACTGAGACGA AGAGTTCTCA GACTGTTCCA GCAGGCAGCT 
CACAAGGTTG TGATTCGACG TCGCATGAAC CGGAGACTGG CTTCTTTGAA GAAACTGGCA 
GACAGTATGA AGAACCTGCC CTTGGCACAG AAAGCTGATA AAGAGAAGAC ATGCGACCTA 
AAAGTGTCTG CAGACAAAGT CTTTCCATCT TCATCCCCCT TTTTCTCTAA TGAAGATGAC 
CCCCTGGCTA ACATTAATTT GCCTGAACTA CCGGTGGATA CTCTTGATGT GACTGTGACA 
TCGCACATTC CTTTCTTCAA GCTACAGGTC CCTCTGCACT ATAAGCTTAT GGGCTACCAG 
GCTGTGTCAG CTTGGGAGGC ATTTAACTCT TATATTCCCA TGACTTTGGC TAGGCCACTG 
CGTACTGGAG CTCCGGACAA GCTGGAACCC AACAGAGCTC ATTTTCCCAT GACAGAGGAC 
ACCACAGCAG GAGAGCAGCA TGATGAACAA GACAAAGATG CAGAAACAAA GGATGATTCC 
AGTCTCTCTT TCTGCGCCCC TGAAAATCTG CTCAGACCTT TTCCAGCAAA CCCACTGAGA 
ATCTTTAACC CAGCTCCGGG ACTTCAGACC TTTAAAACAC CCCCTAAATA TTTGGAGAGT 
GATTTGGAGT TTCATCTCTG CCCTCTGCCC AGGTATACGG TCCCTGAGAG CACATTGTGT 
GGCATAAGGA CCCAAACGCC ACACACTCAG AAGAAGTTTC TCAAATGCAA GGAAGTGATC 
CCTGGGGTTA TGAAGTGGAA AAATTTGGAT TCTATCACCT TGAAAAGTCT TTCAAACCAG 
GCCGCTCTCA CCAGTGACTG TGCTCCACGC AGATCTGACA ACTACAACAC AGAGATACTT 
CCACTCACCG CAGCTTCTCC TCTCGCCAAC CTCCCTGATG ATGATGTGGC CCCGCTAATG 
GACACACTGT GTGAAGGCTC AGGAGTCCAA CTGACACCCG AGATGATCAG AGCAGAATTC 
TTGTGTGGAG AAGTTCTCGT CTCCATTAGC AACCTCGACG CAGGAAAGAC AACCGTGCAC 
CAGAGGCAGC ATCACATTGA GGCGACCTAC AGGTCTGAGT TTAACCAGAT GGGAAAAAGA 
GTGATGACTA GACTGAAGCA ACTTGGAGTC ACTGACAGAA CTTCTCATTT ACCTGAAGAA 
GACCGTGAAA AGCCGAAGCC TGTGCACACA CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552647.2
CDS	166..2679
Translation	MEVALSGPQTLSEPLRRRASLPLSQLVEATQSRGNIPNHLLESKIYANLKSNSLIQAEPSELHFSGFELGKDYVKILKLINISSEVMNIHVIPTQTKYFQTTYTKKYRLIPGLAYTLKVGFCPDEWRYFYDCIRVHCKGEDNLLIPVHAYPVIDDLHIPTHIDLPAVALRQSVCHAIPLRCSCPIGFEFQVHIIQPHESFSIHPLTGVIPAKGEAKITVTFCPLQFETSQITFQLVISQFNTKPYLCTITGSCAPHIALSELEKKFSYESAVTVKHKGASPSTLVLPRSKSKYRANKEADKSKAIRDPTNVKFKLPVDVCTPAGVAKMLIKDSNKLSSKDLKQAMSSGSMIARQNKQIKEALFMKKVQQDMKEEQTNHLKWQVHLGMDPMSEHATRQILEEREIAQHEYMAPEGVPAFQFYTTFQWELRRRVLRLFQQAAHKVVIRRRMNRRLASLKKLADSMKNLPLAQKADKEKTCDLKVSADKVFPSSSPFFSNEDDPLANINLPELPVDTLDVTVTSHIPFFKLQVPLHYKLMGYQAVSAWEAFNSYIPMTLARPLRTGAPDKLEPNRAHFPMTEDTTAGEQHDEQDKDAETKDDSSLSFCAPENLLRPFPANPLRIFNPAPGLQTFKTPPKYLESDLEFHLCPLPRYTVPESTLCGIRTQTPHTQKKFLKCKEVIPGVMKWKNLDSITLKSLSNQAALTSDCAPRRSDNYNTEILPLTAASPLANLPDDDVAPLMDTLCEGSGVQLTPEMIRAEFLCGEVLVSISNLDAGKTTVHQRQHHIEATYRSEFNQMGKRVMTRLKQLGVTDRTSHLPEEDREKPKPVHTHPIHSHE

Target ORF information:

RefSeq Version	XM_004552590.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra cilia and flagella associated protein 221 (cfap221), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552590.2

ORF Insert Sequence:

ATGGAGGTGG CTCTGTCTGG GCCACAGACG CTCTCAGAGC CCCTGAGGAG GAGGGCATCT 
CTACCTCTGA GCCAGCTGGT GGAGGCGACC CAGAGCAGAG GCAACATCCC CAATCATCTG 
CTGGAGTCAA AAATCTATGC TAACCTGAAA AGCAACAGCC TGATCCAAGC AGAGCCCTCT 
GAGCTTCACT TCAGTGGCTT TGAGCTCGGC AAGGATTATG TAAAAATCCT GAAATTAATC 
AACATCTCAT CTGAAGTTAT GAATATTCAT GTAATTCCCA CCCAAACCAA GTACTTCCAA 
ACAACTTATA CCAAAAAGTA TCGACTCATC CCAGGCCTCG CCTACACGCT GAAGGTCGGA 
TTCTGTCCTG ATGAGTGGCG TTACTTCTAT GACTGCATTC GAGTTCACTG TAAGGGGGAA 
GATAACCTGT TAATTCCAGT TCATGCTTAC CCTGTCATCG ATGACCTGCA CATCCCTACT 
CATATCGACC TACCAGCTGT AGCACTCAGA CAAAGTGTTT GCCATGCTAT TCCTCTGAGA 
TGTAGCTGTC CCATTGGCTT TGAGTTTCAA GTGCATATTA TTCAACCCCA TGAGTCCTTC 
TCCATCCACC CCCTCACAGG TGTGATACCA GCCAAAGGTG AGGCAAAGAT AACTGTGACC 
TTTTGTCCAC TCCAGTTTGA GACTTCTCAA ATCACCTTTC AGCTGGTCAT TTCACAGTTC 
AACACCAAGC CTTACCTGTG TACCATCACT GGGAGCTGTG CACCGCACAT AGCTCTCAGT 
GAACTGGAAA AAAAGTTCAG TTATGAAAGT GCAGTGACTG TGAAACACAA AGGAGCCTCA 
CCTTCTACCC TAGTGCTTCC TCGAAGTAAA TCCAAATACA GGGCAAATAA GGAGGCTGAC 
AAGTCAAAGG CAATCAGGGA TCCAACTAAT GTGAAGTTTA AATTGCCAGT AGATGTCTGT 
ACTCCTGCAG GGGTGGCAAA GATGCTGATC AAAGACTCTA ATAAACTCAG CTCTAAAGAC 
TTGAAGCAAG CCATGTCCTC TGGCAGCATG ATAGCACGGC AAAATAAGCA GATAAAAGAA 
GCCCTGTTCA TGAAAAAGGT TCAACAAGAC ATGAAGGAGG AGCAAACAAA TCACCTCAAA 
TGGCAAGTCC ATTTGGGTAT GGACCCCATG TCAGAGCATG CAACAAGACA GATACTGGAG 
GAGAGAGAGA TTGCTCAGCA TGAATATATG GCCCCTGAGG GAGTCCCGGC TTTCCAGTTT 
TACACCACTT TTCAGTGGGA ACTGAGACGA AGAGTTCTCA GACTGTTCCA GCAGGCAGCT 
CACAAGGTTG TGATTCGACG TCGCATGAAC CGGAGACTGG CTTCTTTGAA GAAACTGGCA 
GACAGTATGA AGAACCTGCC CTTGGCACAG AAAGCTGATA AAGAGAAGAC ATGCGACCTA 
AAAGTGTCTG CAGACAAAGT CTTTCCATCT TCATCCCCCT TTTTCTCTAA TGAAGATGAC 
CCCCTGGCTA ACATTAATTT GCCTGAACTA CCGGTGGATA CTCTTGATGT GACTGTGACA 
TCGCACATTC CTTTCTTCAA GCTACAGGTC CCTCTGCACT ATAAGCTTAT GGGCTACCAG 
GCTGTGTCAG CTTGGGAGGC ATTTAACTCT TATATTCCCA TGACTTTGGC TAGGCCACTG 
CGTACTGGAG CTCCGGACAA GCTGGAACCC AACAGAGCTC ATTTTCCCAT GACAGAGGAC 
ACCACAGCAG GAGAGCAGCA TGATGAACAA GACAAAGATG CAGAAACAAA GGATGATTCC 
AGTCTCTCTT TCTGCGCCCC TGAAAATCTG CTCAGACCTT TTCCAGCAAA CCCACTGAGA 
ATCTTTAACC CAGCTCCGGG ACTTCAGACC TTTAAAACAC CCCCTAAATA TTTGGAGAGT 
GATTTGGAGT TTCATCTCTG CCCTCTGCCC AGGTATACGG TCCCTGAGAG CACATTGTGT 
GGCATAAGGA CCCAAACGCC ACACACTCAG AAGAAGTTTC TCAAATGCAA GGAAGTGATC 
CCTGGGGTTA TGAAGTGGAA AAATTTGGAT TCTATCACCT TGAAAAGTCT TTCAAACCAG 
GCCGCTCTCA CCAGTGACTG TGCTCCACGC AGATCTGACA ACTACAACAC AGAGATACTT 
CCACTCACCG CAGCTTCTCC TCTCGCCAAC CTCCCTGATG ATGATGTGGC CCCGCTAATG 
GACACACTGT GTGAAGGCTC AGGAGTCCAA CTGACACCCG AGATGATCAG AGCAGAATTC 
TTGTGTGGAG AAGTTCTCGT CTCCATTAGC AACCTCGACG CAGGAAAGAC AACCGTGCAC 
CAGAGGCAGC ATCACATTGA GGCGACCTAC AGGTCTGAGT TTAACCAGAT GGGAAAAAGA 
GTGATGACTA GACTGAAGCA ACTTGGAGTC ACTGACAGAA CTTCTCATTT ACCTGAAGAA 
GACCGTGAAA AGCCGAAGCC TGTGCACACA CATCCTATAC ACTCACATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cfap221 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム