LOC104925416 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104925416
Entrez Gene ID	104925416
Full Name	calcium-independent phospholipase A2-gamma
Synonyms	pnpla8
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010739032.2	XP_010737334.2	calcium-independent phospholipase A2-gamma isoform X1
XM_010739032.3	XP_010737334.3	calcium-independent phospholipase A2-gamma isoform X1
XM_019272682.1	XP_019128227.1	calcium-independent phospholipase A2-gamma isoform X2
XM_019272683.1	XP_019128228.1	calcium-independent phospholipase A2-gamma isoform X2
XM_019272683.2	XP_019128228.2	calcium-independent phospholipase A2-gamma isoform X2
XM_019272682.2	XP_019128227.2	calcium-independent phospholipase A2-gamma isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC104925416 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104925416 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa106133	XM_010739032.2 Latest version!	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OLa106134	XM_019272683.1 Latest version!	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OLa106134	XM_019272682.1 Latest version!	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OLa213345	XM_010739032.3 Latest version!	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OLa213346	XM_019272682.2 Latest version!	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OLa213346	XM_019272683.2 Latest version!	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104925416 ( XM_010739032.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737334.2 Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa106133
Clone ID Related Accession (Same CDS sequence)	XM_010739032.2
Accession Version	XM_010739032.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	calcium-independent phospholipase A2-gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010739032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCATA GATGTGCTCC AAATGCCTGG GTGAAAGTAA GCAGAGCGTC TCATTTGTGG 
CAAACGCATG CCCACAAGTC AGCGGCATCA CTTCCCTTGT TTGATACCTC TCACTGCAGG 
GTTCATTCAC AACAACCGTA CTGCTACAAG GTTGGCTACC TCAGCTGGAG GCACATCCAC 
CACCTGAGGA TCACTGGGCG CAAGGGATGG CTCTTAAAAG CTAACCAGGT CAGACAAAAA 
CTTGGTCTCC ACACCAGCAG CCTGTATTTG AACACTTCAG CTTCGCGGTG GTCTGCTGGT 
CTGAGCCAAC ACATGTCGCG AGTCAGGAAC ACGTTGGACA CAGTTTCTAA AGCTGTGAGC 
GGGACACATA CAGAACTTCT CTCCAAAATC GCCAGACTCA AGCCCAATGC TTTGAAGGTT 
GGGGAAAAAG CTGAGGCTGG TATTGAGTCG ACAGCAAAAG CTGAGGAGAG TGCAATCACG 
CCTGCCACTG CTTTCACTGA TGCTGCTTCC CCTTCTACGT CTACTCCATC CAAACCCTCG 
TGCGAGTCAC CTTCTTCCAG TCCTTCTATT CCCACGTCTG CTGCTACATA TGACGCAAGT 
GCCACTACCT CTACTCATCC TCATGGTTCT TCCCAAGCGA CCGTCATCGC CACTAATCTG 
CCTGTGCGTG AACCTAAGGA CAAGAGGCTG AGACGTGTTG TTCCTGCTGT CAAAGCCACT 
TGTGCAAGGA AGCAGGAGGA ACCTAAACCT TTACCGAGTG ATACTGAGAG CAAGAGCACA 
GCCTCAAAGC AAACCACAGC ACTTTTCCAT CCCAGCACCT TTTCAGTCAG CCTGGATGAG 
ACCTACAACT ACCTGTCCCA TCACATCAAC TCATACTTTG GCAGCAGCAC AAAGACTCAG 
GATAATAAAG TGGACAATGT TGACCACAGC CCTGCTCCCT CTCAGGGTCA GCAAACTACT 
GACCTCATGT CAGTGTCTGA AAACAAAGAT TCTGCTGTAG CTGCTCCAGT TACGCCACCC 
TCCTCTAAGA AGGGTTTAGG ACACTACCTG TCCTACTCGG CTCCCACCGT ACAAGCCTTT 
GTGGGAAATT ACATAGCTCC GCTGGTCCCC AAGTTTAGGA CAGCAGAGTC CAAAAGTGCT 
GCAGTGGAGG AGAGGAAGTC TGAGGATGCA CCAGTCAAAC AAGTCGAGGC CGCCATTAGT 
AAGGAGCAGA AGGCGGCCGA GGAGAAAGCC AAGAGACTTC TGCTTCAAAG GGAGAAGATC 
ATTGCCAGGG TGAGTGTTGA CAATCGAACC CGGGCTCTGG TACAGGGTCT CCACAGGGCA 
TCTGATGTAA GAGTCTACAT CAACAGGGTG GAGGACCTCA GCTACCATCT CCTGGAGTTT 
CCAGAGACAA GTGGTGTCGC AGTCAAGGAG AAGGTTATCC CTTGCCTACT GCGTCTGAAA 
CAGGCCAGTG ACCCAGGCTT GAGGGCGGCA GTGAGGGAAG CCCTCGCTCT GGTGGGCTAC 
CATGCACCTG TTAAAGGCCG GGGAATACGC ATCCTGTCCA TAGATGGAGG AGGATTGAGG 
GGACTTCTCG CACTTCAGAC GTTACAGAAG CTGGAGGCCC TGACTGGCAA ACCCATTTAC 
AAACTGTTTG ATCATATTTG CGGCGTCAGC ACAGGTGCTG TCCTGGGATT CATGCTGGGC 
GTGTTCCAAA TCCCTCTGAG CGAGTGCGAT GATCTTTACC GGAAGCTGGG GTCGGATGTT 
TTCAAGCAGA ACGTCATCGT TGGCACCGTG AAAATGGGCT GGAACCACGC TTTCTATGAC 
AGCGAAGCCT GGGAGAACAT CCTCAGAGAG AAAATGGGCT CTCACCTTCT GGTCGAGACT 
TCAAGAAACC CTGAATGCCC CAAGGTGGCA GCAGTGAGCA CCATAGTGAA CCGGGGCACT 
CCTCTGAAGG CCTACGTGTT CAGGAACTAC AACCTGCTGC CCGGGGCGCG CTCTCACTAC 
CTGGGGGGCT GCCAACACCA GCTCTGGCAG GCCATCCGTG CCACGTCAGC AGCACCCGGC 
TATTTCCAGG AGTTCACCTT GGGAAATGAT CTCCATCAGG ATGGAGGTCT GCTGATTAAC 
AACCCCACAG CACTGGCTAT CCATGAGTGT AAGTGTTTGT GGCCTAACAC GCCCTTGGAG 
TGTGTGGTCT CACTCGGTAC GGGCCGCTTT GAAACGCACG GCAAAAACAG CAGCACCTAC 
ACGAGCCTCA AAGCCAAACT CAACAATGTC ATCAGCAGTG CCACAGACAC TGAGGAGGTT 
CACGCCATGC TCGACGCCTT CCTCCCTCCC AACACTTATT TCCGCTTCAA CCCGTACTTG 
AGCGAAGACA TCTCCATGGA CGATAACCGG CTGGAAAAGC TCAACTTGCT GCAGGCTCAG 
GGCATCCGTT ATTTGGAGAG GAACGAGGAG AAGCTGAAGA AGGTCACTCG CATCCTCAAC 
CGAGAGAAAA GCTCTCTCCA GAGGATGGCT GAGTGGGCCA GGCTCAGGGT TGACACGTAT 
AACGGTCTGT CCTTTCGGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737334.2
CDS	213..2756
Translation	MSHRCAPNAWVKVSRASHLWQTHAHKSAASLPLFDTSHCRVHSQQPYCYKVGYLSWRHIHHLRITGRKGWLLKANQVRQKLGLHTSSLYLNTSASRWSAGLSQHMSRVRNTLDTVSKAVSGTHTELLSKIARLKPNALKVGEKAEAGIESTAKAEESAITPATAFTDAASPSTSTPSKPSCESPSSSPSIPTSAATYDASATTSTHPHGSSQATVIATNLPVREPKDKRLRRVVPAVKATCARKQEEPKPLPSDTESKSTASKQTTALFHPSTFSVSLDETYNYLSHHINSYFGSSTKTQDNKVDNVDHSPAPSQGQQTTDLMSVSENKDSAVAAPVTPPSSKKGLGHYLSYSAPTVQAFVGNYIAPLVPKFRTAESKSAAVEERKSEDAPVKQVEAAISKEQKAAEEKAKRLLLQREKIIARVSVDNRTRALVQGLHRASDVRVYINRVEDLSYHLLEFPETSGVAVKEKVIPCLLRLKQASDPGLRAAVREALALVGYHAPVKGRGIRILSIDGGGLRGLLALQTLQKLEALTGKPIYKLFDHICGVSTGAVLGFMLGVFQIPLSECDDLYRKLGSDVFKQNVIVGTVKMGWNHAFYDSEAWENILREKMGSHLLVETSRNPECPKVAAVSTIVNRGTPLKAYVFRNYNLLPGARSHYLGGCQHQLWQAIRATSAAPGYFQEFTLGNDLHQDGGLLINNPTALAIHECKCLWPNTPLECVVSLGTGRFETHGKNSSTYTSLKAKLNNVISSATDTEEVHAMLDAFLPPNTYFRFNPYLSEDISMDDNRLEKLNLLQAQGIRYLERNEEKLKKVTRILNREKSSLQRMAEWARLRVDTYNGLSFRS

Target ORF information:

RefSeq Version	XM_010739032.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010739032.2

ORF Insert Sequence:

ATGAGTCATA GATGTGCTCC AAATGCCTGG GTGAAAGTAA GCAGAGCGTC TCATTTGTGG 
CAAACGCATG CCCACAAGTC AGCGGCATCA CTTCCCTTGT TTGATACCTC TCACTGCAGG 
GTTCATTCAC AACAACCGTA CTGCTACAAG GTTGGCTACC TCAGCTGGAG GCACATCCAC 
CACCTGAGGA TCACTGGGCG CAAGGGATGG CTCTTAAAAG CTAACCAGGT CAGACAAAAA 
CTTGGTCTCC ACACCAGCAG CCTGTATTTG AACACTTCAG CTTCGCGGTG GTCTGCTGGT 
CTGAGCCAAC ACATGTCGCG AGTCAGGAAC ACGTTGGACA CAGTTTCTAA AGCTGTGAGC 
GGGACACATA CAGAACTTCT CTCCAAAATC GCCAGACTCA AGCCCAATGC TTTGAAGGTT 
GGGGAAAAAG CTGAGGCTGG TATTGAGTCG ACAGCAAAAG CTGAGGAGAG TGCAATCACG 
CCTGCCACTG CTTTCACTGA TGCTGCTTCC CCTTCTACGT CTACTCCATC CAAACCCTCG 
TGCGAGTCAC CTTCTTCCAG TCCTTCTATT CCCACGTCTG CTGCTACATA TGACGCAAGT 
GCCACTACCT CTACTCATCC TCATGGTTCT TCCCAAGCGA CCGTCATCGC CACTAATCTG 
CCTGTGCGTG AACCTAAGGA CAAGAGGCTG AGACGTGTTG TTCCTGCTGT CAAAGCCACT 
TGTGCAAGGA AGCAGGAGGA ACCTAAACCT TTACCGAGTG ATACTGAGAG CAAGAGCACA 
GCCTCAAAGC AAACCACAGC ACTTTTCCAT CCCAGCACCT TTTCAGTCAG CCTGGATGAG 
ACCTACAACT ACCTGTCCCA TCACATCAAC TCATACTTTG GCAGCAGCAC AAAGACTCAG 
GATAATAAAG TGGACAATGT TGACCACAGC CCTGCTCCCT CTCAGGGTCA GCAAACTACT 
GACCTCATGT CAGTGTCTGA AAACAAAGAT TCTGCTGTAG CTGCTCCAGT TACGCCACCC 
TCCTCTAAGA AGGGTTTAGG ACACTACCTG TCCTACTCGG CTCCCACCGT ACAAGCCTTT 
GTGGGAAATT ACATAGCTCC GCTGGTCCCC AAGTTTAGGA CAGCAGAGTC CAAAAGTGCT 
GCAGTGGAGG AGAGGAAGTC TGAGGATGCA CCAGTCAAAC AAGTCGAGGC CGCCATTAGT 
AAGGAGCAGA AGGCGGCCGA GGAGAAAGCC AAGAGACTTC TGCTTCAAAG GGAGAAGATC 
ATTGCCAGGG TGAGTGTTGA CAATCGAACC CGGGCTCTGG TACAGGGTCT CCACAGGGCA 
TCTGATGTAA GAGTCTACAT CAACAGGGTG GAGGACCTCA GCTACCATCT CCTGGAGTTT 
CCAGAGACAA GTGGTGTCGC AGTCAAGGAG AAGGTTATCC CTTGCCTACT GCGTCTGAAA 
CAGGCCAGTG ACCCAGGCTT GAGGGCGGCA GTGAGGGAAG CCCTCGCTCT GGTGGGCTAC 
CATGCACCTG TTAAAGGCCG GGGAATACGC ATCCTGTCCA TAGATGGAGG AGGATTGAGG 
GGACTTCTCG CACTTCAGAC GTTACAGAAG CTGGAGGCCC TGACTGGCAA ACCCATTTAC 
AAACTGTTTG ATCATATTTG CGGCGTCAGC ACAGGTGCTG TCCTGGGATT CATGCTGGGC 
GTGTTCCAAA TCCCTCTGAG CGAGTGCGAT GATCTTTACC GGAAGCTGGG GTCGGATGTT 
TTCAAGCAGA ACGTCATCGT TGGCACCGTG AAAATGGGCT GGAACCACGC TTTCTATGAC 
AGCGAAGCCT GGGAGAACAT CCTCAGAGAG AAAATGGGCT CTCACCTTCT GGTCGAGACT 
TCAAGAAACC CTGAATGCCC CAAGGTGGCA GCAGTGAGCA CCATAGTGAA CCGGGGCACT 
CCTCTGAAGG CCTACGTGTT CAGGAACTAC AACCTGCTGC CCGGGGCGCG CTCTCACTAC 
CTGGGGGGCT GCCAACACCA GCTCTGGCAG GCCATCCGTG CCACGTCAGC AGCACCCGGC 
TATTTCCAGG AGTTCACCTT GGGAAATGAT CTCCATCAGG ATGGAGGTCT GCTGATTAAC 
AACCCCACAG CACTGGCTAT CCATGAGTGT AAGTGTTTGT GGCCTAACAC GCCCTTGGAG 
TGTGTGGTCT CACTCGGTAC GGGCCGCTTT GAAACGCACG GCAAAAACAG CAGCACCTAC 
ACGAGCCTCA AAGCCAAACT CAACAATGTC ATCAGCAGTG CCACAGACAC TGAGGAGGTT 
CACGCCATGC TCGACGCCTT CCTCCCTCCC AACACTTATT TCCGCTTCAA CCCGTACTTG 
AGCGAAGACA TCTCCATGGA CGATAACCGG CTGGAAAAGC TCAACTTGCT GCAGGCTCAG 
GGCATCCGTT ATTTGGAGAG GAACGAGGAG AAGCTGAAGA AGGTCACTCG CATCCTCAAC 
CGAGAGAAAA GCTCTCTCCA GAGGATGGCT GAGTGGGCCA GGCTCAGGGT TGACACGTAT 
AACGGTCTGT CCTTTCGGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104925416 ( XM_019272682.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128227.1 Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa106134
Clone ID Related Accession (Same CDS sequence)	XM_019272682.1 , XM_019272683.1
Accession Version	XM_019272682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	calcium-independent phospholipase A2-gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCAGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACCGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCATTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAAAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAAAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128227.1
CDS	341..2572
Translation	MSRVRNTLDTVSKAVSGTHTELLSKIARLKPNALKVGEKAEAGIESTAKAEESAITPATAFTDAASPSTSTPSKPSCESPSSSPSIPTSAATYDASATTSTHPHGSSQATVIATNLPVREPKDKRLRRVVPAVKATCARKQEEPKPLPSDTESKSTASKQTTALFHPSTFSVSLDETYNYLSHHINSYFGSSTKTQDNKVDNVDHSPAPSQGQQTTDLMSVSENKDSAVAAPVTPPSSKKGLGHYLSYSAPTVQAFVGNYIAPLVPKFRTAESKSAAVEERKSEDAPVKQVEAAISKEQKAAEEKAKRLLLQREKIIARVSVDNRTRALVQGLHRASDVRVYINRVEDLSYHLLEFPETSGVAVKEKVIPCLLRLKQASDPGLRAAVREALALVGYHAPVKGRGIRILSIDGGGLRGLLALQTLQKLEALTGKPIYKLFDHICGVSTGAVLGFMLGVFQIPLSECDDLYRKLGSDVFKQNVIVGTVKMGWNHAFYDSEAWENILREKMGSHLLVETSRNPECPKVAAVSTIVNRGTPLKAYVFRNYNLLPGARSHYLGGCQHQLWQAIRATSAAPGYFQEFTLGNDLHQDGGLLINNPTALAIHECKCLWPNTPLECVVSLGTGRFETHGKNSSTYTSLKAKLNNVISSATDTEEVHAMLDAFLPPNTYFRFNPYLSEDISMDDNRLEKLNLLQAQGIRYLERNEEKLKKVTRILNREKSSLQRMAEWARLRVDTYNGLSFRS

Target ORF information:

RefSeq Version	XM_019272682.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272682.1

ORF Insert Sequence:

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCAGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACCGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCATTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAAAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAAAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104925416 ( XM_019272683.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128228.1 Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X3, mRNA.

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CloneID	OLa106134
Clone ID Related Accession (Same CDS sequence)	XM_019272682.1 , XM_019272683.1
Accession Version	XM_019272683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	calcium-independent phospholipase A2-gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCAGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACCGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCATTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAAAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAAAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128228.1
CDS	442..2673
Translation	MSRVRNTLDTVSKAVSGTHTELLSKIARLKPNALKVGEKAEAGIESTAKAEESAITPATAFTDAASPSTSTPSKPSCESPSSSPSIPTSAATYDASATTSTHPHGSSQATVIATNLPVREPKDKRLRRVVPAVKATCARKQEEPKPLPSDTESKSTASKQTTALFHPSTFSVSLDETYNYLSHHINSYFGSSTKTQDNKVDNVDHSPAPSQGQQTTDLMSVSENKDSAVAAPVTPPSSKKGLGHYLSYSAPTVQAFVGNYIAPLVPKFRTAESKSAAVEERKSEDAPVKQVEAAISKEQKAAEEKAKRLLLQREKIIARVSVDNRTRALVQGLHRASDVRVYINRVEDLSYHLLEFPETSGVAVKEKVIPCLLRLKQASDPGLRAAVREALALVGYHAPVKGRGIRILSIDGGGLRGLLALQTLQKLEALTGKPIYKLFDHICGVSTGAVLGFMLGVFQIPLSECDDLYRKLGSDVFKQNVIVGTVKMGWNHAFYDSEAWENILREKMGSHLLVETSRNPECPKVAAVSTIVNRGTPLKAYVFRNYNLLPGARSHYLGGCQHQLWQAIRATSAAPGYFQEFTLGNDLHQDGGLLINNPTALAIHECKCLWPNTPLECVVSLGTGRFETHGKNSSTYTSLKAKLNNVISSATDTEEVHAMLDAFLPPNTYFRFNPYLSEDISMDDNRLEKLNLLQAQGIRYLERNEEKLKKVTRILNREKSSLQRMAEWARLRVDTYNGLSFRS

Target ORF information:

RefSeq Version	XM_019272683.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272683.1

ORF Insert Sequence:

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCAGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACCGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCATTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAAAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAAAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104925416 ( XM_010739032.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737334.3 Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X1, mRNA.

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CloneID	OLa213345
Clone ID Related Accession (Same CDS sequence)	XM_010739032.3
Accession Version	XM_010739032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	calcium-independent phospholipase A2-gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010739032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCATA GATGTGCTCC AAATGCCTGG GTGAAAGTAA GCAGAGCGTC TCATTTGTGG 
CAAACGCATG CCCACAAGTC AGCGGCATCA CTTCCCTTGT TTGATACCTC TCACTGCAGG 
GTTCATTCAC AACAACCGTA CTGCTACAAG GTTGGCTACC TCAGCTGGAG GCACATCCAC 
CACCTGAGGA TCACTGGGCG CAAGGGATGG CTCTTAAAAG CTAACCAGGT CAGACAAAAA 
CTTGGTCTCC ACACCAGCAG CCTGTATTTG AACACTTCAG CTTCGCGGTG GTCTGCTGGT 
CTGAGCCAAC ACATGTCGCG AGTCAGGAAC ACGTTGGACA CAGTTTCTAA AGCTGTGAGC 
GGGACACATA CAGAACTTCT CTCCAAAATC GCCAGACTCA AGCCCAATGC TTTGAAGGTT 
GGGGAAAAAG CTGAGGCTGG TATTGAGTCG ACAGCAAAAG CTGAGGAGAG TGCAATCACG 
CCTGCCACTG CTTTCACTGA TGCTGCTTCC CCTTCTACGT CTACTCCATC CAAACCCTCG 
TGCGAGTCAC CTTCTTCCAG TCCTTCTATT CCCACGTCTG CTGCTACATA TGACGCAAGT 
GCCACTACCT CTACTCATCC TCATGGTTCT TCCCAAGCGA CCGTCATCGC CACTAATCTG 
CCTGTGCGTG AACCTAAGGA CAAGAGGCTG AGACGTGTTG TTCCTGCTGT CAAAGCCACT 
TGTGCAAGGA AGCAGGAGGA ACCTAAACCT TTACCGAGTG ATACTGAGAG CAAGAGCACA 
GCCTCAAAGC AAACCACAGC ACTTTTCCAT CCCAGCACCT TTTCAGTCAG CCTGGATGAG 
ACCTACAACT ACCTGTCCCA TCACATCAAC TCATACTTTG GCAGCAGCAC AAAGACTCAG 
GATAATAAAG TGGACAATGT TGACCACAGC CCTGCTCCCT CTCAGGGTCA GCAAACTACT 
GACCTCATGT CAGTGTCTGA AAACAAAGAT TCTGCTGTAG CTGCTCCGGT TACGCCACCC 
TCCTCTAAGA AGGGTTTAGG ACACTACCTG TCCTACTCGG CTCCCACTGT ACAAGCCTTT 
GTGGGAAATT ACATAGCTCC GCTGGTCCCC AAGTTTAGGA CAGCAGAGTC CAAAAGTGCT 
GCAGTGGAGG AGAGGAAGTC TGAGGATGCA CCAGTCAAAC AAGTCGAGGC CGCCGTTAGT 
AAGGAGCAGA AGGCGGCCGA GGAGAAAGCC AAGAGACTTC TGCTTCAAAG GGAGAAGATC 
ATTGCCAGGG TGAGTGTTGA CAATCGAACC CGGGCTCTGG TACAGGGTCT CCACAGGGCA 
TCTGATGTAA GAGTCTACAT CAACAGGGTG GAGGACCTCA GCTACCATCT CCTGGAGTTT 
CCAGAGACAA GTGGTGTCGC AGTCAAGGAG AAGGTTATCC CTTGCCTACT GCGTCTGAAA 
CAGGCCAGTG ACCCAGGCTT GAGGGCGGCA GTGAGGGAAG CCCTCGCTCT GGTGGGCTAC 
CATGCACCTG TTAAAGGCCG GGGAATACGC ATCCTGTCCA TAGATGGAGG AGGATTGAGG 
GGACTTCTCG CACTTCAGAC GTTACAGAAG CTGGAGGCCC TGACTGGCAA ACCCATTTAC 
AAACTGTTTG ATCATATTTG CGGCGTCAGC ACAGGTGCTG TCCTGGGATT CATGCTGGGC 
GTGTTCCAAA TCCCTCTGAG CGAGTGCGAT GATCTTTACC GGAAGCTGGG GTCGGATGTT 
TTCAAGCAGA ACGTCATCGT TGGCACCGTG AAAATGGGCT GGAACCACGC TTTCTATGAC 
AGCGAAGCCT GGGAGAACAT CCTCAGAGAG AAAATGGGCT CTCACCTTCT GGTCGAGACT 
TCAAGAAACC CTGAATGCCC CAAGGTGGCA GCAGTGAGCA CCATAGTGAA CCGGGGCACT 
CCTCTGAAGG CCTACGTGTT CAGGAACTAC AACCTGCTGC CCGGGGCGCG CTCTCACTAC 
CTGGGGGGCT GCCAACACCA GCTCTGGCAG GCCATCCGTG CCACGTCAGC AGCACCCGGC 
TATTTCCAGG AGTTCACCTT GGGAAATGAT CTCCATCAGG ATGGAGGTCT GCTGATTAAC 
AACCCCACAG CACTGGCTAT CCATGAGTGT AAGTGTTTGT GGCCTAACAC GCCCTTGGAG 
TGTGTGGTCT CACTCGGTAC GGGCCGCTTT GAAACGCACG GCAAGAACAG CAGCACCTAC 
ACGAGCCTCA AAGCCAAACT CAACAATGTC ATCAGCAGTG CCACAGACAC TGAGGAGGTT 
CACGCCATGC TCGACGCCTT CCTCCCTCCC AACACTTATT TCCGCTTCAA CCCGTACTTG 
AGCGAAGACA TCTCCATGGA CGATAACCGG CTGGAGAAGC TCAACTTGCT GCAGGCTCAG 
GGCATCCGTT ATTTGGAGAG GAACGAGGAG AAGCTGAAGA AGGTCACTCG CATCCTCAAC 
CGAGAGAAAA GCTCTCTCCA GAGGATGGCT GAGTGGGCCA GGCTCAGGGT TGACACGTAT 
AACGGTCTGT CCTTTCGGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737334.3
CDS	300..2843
Translation	MSHRCAPNAWVKVSRASHLWQTHAHKSAASLPLFDTSHCRVHSQQPYCYKVGYLSWRHIHHLRITGRKGWLLKANQVRQKLGLHTSSLYLNTSASRWSAGLSQHMSRVRNTLDTVSKAVSGTHTELLSKIARLKPNALKVGEKAEAGIESTAKAEESAITPATAFTDAASPSTSTPSKPSCESPSSSPSIPTSAATYDASATTSTHPHGSSQATVIATNLPVREPKDKRLRRVVPAVKATCARKQEEPKPLPSDTESKSTASKQTTALFHPSTFSVSLDETYNYLSHHINSYFGSSTKTQDNKVDNVDHSPAPSQGQQTTDLMSVSENKDSAVAAPVTPPSSKKGLGHYLSYSAPTVQAFVGNYIAPLVPKFRTAESKSAAVEERKSEDAPVKQVEAAVSKEQKAAEEKAKRLLLQREKIIARVSVDNRTRALVQGLHRASDVRVYINRVEDLSYHLLEFPETSGVAVKEKVIPCLLRLKQASDPGLRAAVREALALVGYHAPVKGRGIRILSIDGGGLRGLLALQTLQKLEALTGKPIYKLFDHICGVSTGAVLGFMLGVFQIPLSECDDLYRKLGSDVFKQNVIVGTVKMGWNHAFYDSEAWENILREKMGSHLLVETSRNPECPKVAAVSTIVNRGTPLKAYVFRNYNLLPGARSHYLGGCQHQLWQAIRATSAAPGYFQEFTLGNDLHQDGGLLINNPTALAIHECKCLWPNTPLECVVSLGTGRFETHGKNSSTYTSLKAKLNNVISSATDTEEVHAMLDAFLPPNTYFRFNPYLSEDISMDDNRLEKLNLLQAQGIRYLERNEEKLKKVTRILNREKSSLQRMAEWARLRVDTYNGLSFRS

Target ORF information:

RefSeq Version	XM_010739032.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010739032.3

ORF Insert Sequence:

ATGAGTCATA GATGTGCTCC AAATGCCTGG GTGAAAGTAA GCAGAGCGTC TCATTTGTGG 
CAAACGCATG CCCACAAGTC AGCGGCATCA CTTCCCTTGT TTGATACCTC TCACTGCAGG 
GTTCATTCAC AACAACCGTA CTGCTACAAG GTTGGCTACC TCAGCTGGAG GCACATCCAC 
CACCTGAGGA TCACTGGGCG CAAGGGATGG CTCTTAAAAG CTAACCAGGT CAGACAAAAA 
CTTGGTCTCC ACACCAGCAG CCTGTATTTG AACACTTCAG CTTCGCGGTG GTCTGCTGGT 
CTGAGCCAAC ACATGTCGCG AGTCAGGAAC ACGTTGGACA CAGTTTCTAA AGCTGTGAGC 
GGGACACATA CAGAACTTCT CTCCAAAATC GCCAGACTCA AGCCCAATGC TTTGAAGGTT 
GGGGAAAAAG CTGAGGCTGG TATTGAGTCG ACAGCAAAAG CTGAGGAGAG TGCAATCACG 
CCTGCCACTG CTTTCACTGA TGCTGCTTCC CCTTCTACGT CTACTCCATC CAAACCCTCG 
TGCGAGTCAC CTTCTTCCAG TCCTTCTATT CCCACGTCTG CTGCTACATA TGACGCAAGT 
GCCACTACCT CTACTCATCC TCATGGTTCT TCCCAAGCGA CCGTCATCGC CACTAATCTG 
CCTGTGCGTG AACCTAAGGA CAAGAGGCTG AGACGTGTTG TTCCTGCTGT CAAAGCCACT 
TGTGCAAGGA AGCAGGAGGA ACCTAAACCT TTACCGAGTG ATACTGAGAG CAAGAGCACA 
GCCTCAAAGC AAACCACAGC ACTTTTCCAT CCCAGCACCT TTTCAGTCAG CCTGGATGAG 
ACCTACAACT ACCTGTCCCA TCACATCAAC TCATACTTTG GCAGCAGCAC AAAGACTCAG 
GATAATAAAG TGGACAATGT TGACCACAGC CCTGCTCCCT CTCAGGGTCA GCAAACTACT 
GACCTCATGT CAGTGTCTGA AAACAAAGAT TCTGCTGTAG CTGCTCCGGT TACGCCACCC 
TCCTCTAAGA AGGGTTTAGG ACACTACCTG TCCTACTCGG CTCCCACTGT ACAAGCCTTT 
GTGGGAAATT ACATAGCTCC GCTGGTCCCC AAGTTTAGGA CAGCAGAGTC CAAAAGTGCT 
GCAGTGGAGG AGAGGAAGTC TGAGGATGCA CCAGTCAAAC AAGTCGAGGC CGCCGTTAGT 
AAGGAGCAGA AGGCGGCCGA GGAGAAAGCC AAGAGACTTC TGCTTCAAAG GGAGAAGATC 
ATTGCCAGGG TGAGTGTTGA CAATCGAACC CGGGCTCTGG TACAGGGTCT CCACAGGGCA 
TCTGATGTAA GAGTCTACAT CAACAGGGTG GAGGACCTCA GCTACCATCT CCTGGAGTTT 
CCAGAGACAA GTGGTGTCGC AGTCAAGGAG AAGGTTATCC CTTGCCTACT GCGTCTGAAA 
CAGGCCAGTG ACCCAGGCTT GAGGGCGGCA GTGAGGGAAG CCCTCGCTCT GGTGGGCTAC 
CATGCACCTG TTAAAGGCCG GGGAATACGC ATCCTGTCCA TAGATGGAGG AGGATTGAGG 
GGACTTCTCG CACTTCAGAC GTTACAGAAG CTGGAGGCCC TGACTGGCAA ACCCATTTAC 
AAACTGTTTG ATCATATTTG CGGCGTCAGC ACAGGTGCTG TCCTGGGATT CATGCTGGGC 
GTGTTCCAAA TCCCTCTGAG CGAGTGCGAT GATCTTTACC GGAAGCTGGG GTCGGATGTT 
TTCAAGCAGA ACGTCATCGT TGGCACCGTG AAAATGGGCT GGAACCACGC TTTCTATGAC 
AGCGAAGCCT GGGAGAACAT CCTCAGAGAG AAAATGGGCT CTCACCTTCT GGTCGAGACT 
TCAAGAAACC CTGAATGCCC CAAGGTGGCA GCAGTGAGCA CCATAGTGAA CCGGGGCACT 
CCTCTGAAGG CCTACGTGTT CAGGAACTAC AACCTGCTGC CCGGGGCGCG CTCTCACTAC 
CTGGGGGGCT GCCAACACCA GCTCTGGCAG GCCATCCGTG CCACGTCAGC AGCACCCGGC 
TATTTCCAGG AGTTCACCTT GGGAAATGAT CTCCATCAGG ATGGAGGTCT GCTGATTAAC 
AACCCCACAG CACTGGCTAT CCATGAGTGT AAGTGTTTGT GGCCTAACAC GCCCTTGGAG 
TGTGTGGTCT CACTCGGTAC GGGCCGCTTT GAAACGCACG GCAAGAACAG CAGCACCTAC 
ACGAGCCTCA AAGCCAAACT CAACAATGTC ATCAGCAGTG CCACAGACAC TGAGGAGGTT 
CACGCCATGC TCGACGCCTT CCTCCCTCCC AACACTTATT TCCGCTTCAA CCCGTACTTG 
AGCGAAGACA TCTCCATGGA CGATAACCGG CTGGAGAAGC TCAACTTGCT GCAGGCTCAG 
GGCATCCGTT ATTTGGAGAG GAACGAGGAG AAGCTGAAGA AGGTCACTCG CATCCTCAAC 
CGAGAGAAAA GCTCTCTCCA GAGGATGGCT GAGTGGGCCA GGCTCAGGGT TGACACGTAT 
AACGGTCTGT CCTTTCGGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104925416 ( XM_019272683.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128228.2 Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa213346
Clone ID Related Accession (Same CDS sequence)	XM_019272683.2 , XM_019272682.2
Accession Version	XM_019272683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	calcium-independent phospholipase A2-gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019272683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCGGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACTGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCGTTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAGAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAGAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128228.2
CDS	529..2760
Translation	MSRVRNTLDTVSKAVSGTHTELLSKIARLKPNALKVGEKAEAGIESTAKAEESAITPATAFTDAASPSTSTPSKPSCESPSSSPSIPTSAATYDASATTSTHPHGSSQATVIATNLPVREPKDKRLRRVVPAVKATCARKQEEPKPLPSDTESKSTASKQTTALFHPSTFSVSLDETYNYLSHHINSYFGSSTKTQDNKVDNVDHSPAPSQGQQTTDLMSVSENKDSAVAAPVTPPSSKKGLGHYLSYSAPTVQAFVGNYIAPLVPKFRTAESKSAAVEERKSEDAPVKQVEAAVSKEQKAAEEKAKRLLLQREKIIARVSVDNRTRALVQGLHRASDVRVYINRVEDLSYHLLEFPETSGVAVKEKVIPCLLRLKQASDPGLRAAVREALALVGYHAPVKGRGIRILSIDGGGLRGLLALQTLQKLEALTGKPIYKLFDHICGVSTGAVLGFMLGVFQIPLSECDDLYRKLGSDVFKQNVIVGTVKMGWNHAFYDSEAWENILREKMGSHLLVETSRNPECPKVAAVSTIVNRGTPLKAYVFRNYNLLPGARSHYLGGCQHQLWQAIRATSAAPGYFQEFTLGNDLHQDGGLLINNPTALAIHECKCLWPNTPLECVVSLGTGRFETHGKNSSTYTSLKAKLNNVISSATDTEEVHAMLDAFLPPNTYFRFNPYLSEDISMDDNRLEKLNLLQAQGIRYLERNEEKLKKVTRILNREKSSLQRMAEWARLRVDTYNGLSFRS

Target ORF information:

RefSeq Version	XM_019272683.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272683.2

ORF Insert Sequence:

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCGGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACTGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCGTTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAGAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAGAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104925416 ( XM_019272682.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128227.2 Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X3, mRNA.

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CloneID	OLa213346
Clone ID Related Accession (Same CDS sequence)	XM_019272683.2 , XM_019272682.2
Accession Version	XM_019272682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	calcium-independent phospholipase A2-gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019272682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCGGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACTGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCGTTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAGAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAGAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128227.2
CDS	428..2659
Translation	MSRVRNTLDTVSKAVSGTHTELLSKIARLKPNALKVGEKAEAGIESTAKAEESAITPATAFTDAASPSTSTPSKPSCESPSSSPSIPTSAATYDASATTSTHPHGSSQATVIATNLPVREPKDKRLRRVVPAVKATCARKQEEPKPLPSDTESKSTASKQTTALFHPSTFSVSLDETYNYLSHHINSYFGSSTKTQDNKVDNVDHSPAPSQGQQTTDLMSVSENKDSAVAAPVTPPSSKKGLGHYLSYSAPTVQAFVGNYIAPLVPKFRTAESKSAAVEERKSEDAPVKQVEAAVSKEQKAAEEKAKRLLLQREKIIARVSVDNRTRALVQGLHRASDVRVYINRVEDLSYHLLEFPETSGVAVKEKVIPCLLRLKQASDPGLRAAVREALALVGYHAPVKGRGIRILSIDGGGLRGLLALQTLQKLEALTGKPIYKLFDHICGVSTGAVLGFMLGVFQIPLSECDDLYRKLGSDVFKQNVIVGTVKMGWNHAFYDSEAWENILREKMGSHLLVETSRNPECPKVAAVSTIVNRGTPLKAYVFRNYNLLPGARSHYLGGCQHQLWQAIRATSAAPGYFQEFTLGNDLHQDGGLLINNPTALAIHECKCLWPNTPLECVVSLGTGRFETHGKNSSTYTSLKAKLNNVISSATDTEEVHAMLDAFLPPNTYFRFNPYLSEDISMDDNRLEKLNLLQAQGIRYLERNEEKLKKVTRILNREKSSLQRMAEWARLRVDTYNGLSFRS

Target ORF information:

RefSeq Version	XM_019272682.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea calcium-independent phospholipase A2-gamma (LOC104925416), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272682.2

ORF Insert Sequence:

ATGTCGCGAG TCAGGAACAC GTTGGACACA GTTTCTAAAG CTGTGAGCGG GACACATACA 
GAACTTCTCT CCAAAATCGC CAGACTCAAG CCCAATGCTT TGAAGGTTGG GGAAAAAGCT 
GAGGCTGGTA TTGAGTCGAC AGCAAAAGCT GAGGAGAGTG CAATCACGCC TGCCACTGCT 
TTCACTGATG CTGCTTCCCC TTCTACGTCT ACTCCATCCA AACCCTCGTG CGAGTCACCT 
TCTTCCAGTC CTTCTATTCC CACGTCTGCT GCTACATATG ACGCAAGTGC CACTACCTCT 
ACTCATCCTC ATGGTTCTTC CCAAGCGACC GTCATCGCCA CTAATCTGCC TGTGCGTGAA 
CCTAAGGACA AGAGGCTGAG ACGTGTTGTT CCTGCTGTCA AAGCCACTTG TGCAAGGAAG 
CAGGAGGAAC CTAAACCTTT ACCGAGTGAT ACTGAGAGCA AGAGCACAGC CTCAAAGCAA 
ACCACAGCAC TTTTCCATCC CAGCACCTTT TCAGTCAGCC TGGATGAGAC CTACAACTAC 
CTGTCCCATC ACATCAACTC ATACTTTGGC AGCAGCACAA AGACTCAGGA TAATAAAGTG 
GACAATGTTG ACCACAGCCC TGCTCCCTCT CAGGGTCAGC AAACTACTGA CCTCATGTCA 
GTGTCTGAAA ACAAAGATTC TGCTGTAGCT GCTCCGGTTA CGCCACCCTC CTCTAAGAAG 
GGTTTAGGAC ACTACCTGTC CTACTCGGCT CCCACTGTAC AAGCCTTTGT GGGAAATTAC 
ATAGCTCCGC TGGTCCCCAA GTTTAGGACA GCAGAGTCCA AAAGTGCTGC AGTGGAGGAG 
AGGAAGTCTG AGGATGCACC AGTCAAACAA GTCGAGGCCG CCGTTAGTAA GGAGCAGAAG 
GCGGCCGAGG AGAAAGCCAA GAGACTTCTG CTTCAAAGGG AGAAGATCAT TGCCAGGGTG 
AGTGTTGACA ATCGAACCCG GGCTCTGGTA CAGGGTCTCC ACAGGGCATC TGATGTAAGA 
GTCTACATCA ACAGGGTGGA GGACCTCAGC TACCATCTCC TGGAGTTTCC AGAGACAAGT 
GGTGTCGCAG TCAAGGAGAA GGTTATCCCT TGCCTACTGC GTCTGAAACA GGCCAGTGAC 
CCAGGCTTGA GGGCGGCAGT GAGGGAAGCC CTCGCTCTGG TGGGCTACCA TGCACCTGTT 
AAAGGCCGGG GAATACGCAT CCTGTCCATA GATGGAGGAG GATTGAGGGG ACTTCTCGCA 
CTTCAGACGT TACAGAAGCT GGAGGCCCTG ACTGGCAAAC CCATTTACAA ACTGTTTGAT 
CATATTTGCG GCGTCAGCAC AGGTGCTGTC CTGGGATTCA TGCTGGGCGT GTTCCAAATC 
CCTCTGAGCG AGTGCGATGA TCTTTACCGG AAGCTGGGGT CGGATGTTTT CAAGCAGAAC 
GTCATCGTTG GCACCGTGAA AATGGGCTGG AACCACGCTT TCTATGACAG CGAAGCCTGG 
GAGAACATCC TCAGAGAGAA AATGGGCTCT CACCTTCTGG TCGAGACTTC AAGAAACCCT 
GAATGCCCCA AGGTGGCAGC AGTGAGCACC ATAGTGAACC GGGGCACTCC TCTGAAGGCC 
TACGTGTTCA GGAACTACAA CCTGCTGCCC GGGGCGCGCT CTCACTACCT GGGGGGCTGC 
CAACACCAGC TCTGGCAGGC CATCCGTGCC ACGTCAGCAG CACCCGGCTA TTTCCAGGAG 
TTCACCTTGG GAAATGATCT CCATCAGGAT GGAGGTCTGC TGATTAACAA CCCCACAGCA 
CTGGCTATCC ATGAGTGTAA GTGTTTGTGG CCTAACACGC CCTTGGAGTG TGTGGTCTCA 
CTCGGTACGG GCCGCTTTGA AACGCACGGC AAGAACAGCA GCACCTACAC GAGCCTCAAA 
GCCAAACTCA ACAATGTCAT CAGCAGTGCC ACAGACACTG AGGAGGTTCA CGCCATGCTC 
GACGCCTTCC TCCCTCCCAA CACTTATTTC CGCTTCAACC CGTACTTGAG CGAAGACATC 
TCCATGGACG ATAACCGGCT GGAGAAGCTC AACTTGCTGC AGGCTCAGGG CATCCGTTAT 
TTGGAGAGGA ACGAGGAGAA GCTGAAGAAG GTCACTCGCA TCCTCAACCG AGAGAAAAGC 
TCTCTCCAGA GGATGGCTGA GTGGGCCAGG CTCAGGGTTG ACACGTATAA CGGTCTGTCC 
TTTCGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC104925416 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム