EDEM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM1
Entrez Gene ID	9695
Full Name	ER degradation enhancing alpha-mannosidase like protein 1
Synonyms	EDEM
General protein information	Preferred Names ER degradation enhancing alpha-mannosidase like protein 1 Names ER degradation-enhancing alpha-mannosidase-like protein 1 ER degradation enhancer, mannosidase alpha-like 1 ER degradation-enhancing alpha-mannosidase-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p26.1
Disorder MIM:	607673

mRNA and Protein(s)

mRNA	Protein	Name
XM_011534271.2	XP_011532573.1	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
NM_014674.3	NP_055489.1	ER degradation-enhancing alpha-mannosidase-like protein 1
NM_014674.2	NP_055489.1	ER degradation-enhancing alpha-mannosidase-like protein 1
XM_011534271.3	XP_011532573.1	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
XM_011534272.2	XP_011532574.1	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Edem1	XP_238366.4
	EDEM1	XP_001098603.1
Canis lupus familiaris (dog)	EDEM1	XP_533753.2
Bos taurus (cattle)	EDEM1	NP_001096562.1
Mus musculus (house mouse)	Edem1	NP_619618.1
Gallus gallus (chicken)	EDEM1	NP_001006143.1
Homo sapiens (human)	EDEM1	NP_055489.1
Danio rerio (zebrafish)	edem1	NP_957483.1
Arabidopsis thaliana (thale cress)	AT1G27520	NP_564288.1
Oryza sativa (rice)	Os02g0741300	NP_001048080.1
Xenopus tropicalis (tropical clawed frog)	edem1	NP_989397.1
Pan troglodytes (chimpanzee)	EDEM1	XP_003309640.1
Caenorhabditis elegans (roundworm)	C47E12.3	NP_501802.2
Schizosaccharomyces pombe (fission yeast)	mnl1	NP_594434.1

Gene Ontology
Bibliography

Related articles in PubMed

Endoplasmic reticulum degradation-enhancing ?-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation.
Guiliano DB, Fussell H, Lenart I, Tsao E, Nesbeth D, Fletcher AJ, Campbell EC, Yousaf N, Williams S, Santos S, Cameron A, Towers GJ, Kellam P, Hebert DN, Gould KG, Powis SJ, Antoniou AN
Arthritis & rheumatology (Hoboken, N.J.)66(11)2976-88(2014 Nov)

MicroRNA-581 promotes hepatitis B virus surface antigen expression by targeting Dicer and EDEM1.
Wang YQ, Ren YF, Song YJ, Xue YF, Zhang XJ, Cao ST, Deng ZJ, Wu J, Chen L, Li G, Shi KQ, Chen YP, Ren H, Huang AL, Tang KF
Carcinogenesis35(9)2127-33(2014 Sep)

EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.
Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
The Journal of cell biology206(3)347-56(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation.
Title: Endoplasmic reticulum degradation-enhancing ?-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation.

miR-581 promotes hepatitis B virus surface antigen expression by targeting Dicer and EDEM1 in HepG2 cells.
Title: MicroRNA-581 promotes hepatitis B virus surface antigen expression by targeting Dicer and EDEM1.

EDEM1 colocalizes with the selective autophagy cargo receptors p62/SQSTM1, neighbor of BRCA1 gene 1 (NBR1) and autophagy-linked FYVE (Alfy) protein, and becomes engulfed by autophagic isolation membranes.
Title: ERADication of EDEM1 occurs by selective autophagy and requires deglycosylation by cytoplasmic peptide N-glycanase.

EDEM1 associates with lifespan in animal models, although not humans.
Title: Identification of genes promoting skin youthfulness by genome-wide association study.

analysis of a shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins
Title: A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins.

The following EDEM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15926	NM_014674.3 Latest version!	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu63164	XM_011534271.3 Latest version!	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu63164	XM_011534272.2 Latest version!	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu15926	NM_014674.2	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu63164	XM_011534271.2	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM1 ( NM_014674.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055489.1 Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), mRNA.

Price & Availability↑

CloneID	OHu15926
Clone ID Related Accession (Same CDS sequence)	NM_014674.3 , NM_014674.2
Accession Version	NM_014674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC019088.1, AK292643.1, AC026202.7 and CN364698.1. On Nov 23, 2018 this sequence version replaced NM_014674.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86967.1, SRR1803612.130328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000256497.9/ ENSP00000256497.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAATGGC GAGCGCTCGT CCTGGGGCTG GTGCTCCTCC GGCTTGGCCT CCATGGAGTA 
TTGTGGCTCG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGCTT TCCGCTCAGC 
TTCGGCTTCC AGCGTCTGAG GAGCCCCGAC GGCCCCGCGT CGCCCACCTC GGGGCCCGTG 
GGCCGGCCTG GGGGGGTATC CGGGCCGTCG TGGCTGCAGC CGCCGGGGAC CGGGGCAGCG 
CAGAGCCCGC GCAAGGCTCC GCGGCGTCCT GGGCCGGGGA TGTGCGGCCC AGCCAACTGG 
GGCTACGTGC TGGGCGGCCG GGGCCGCGGC CCGGACGAGT ACGAGAAGCG CTACAGCGGC 
GCCTTCCCTC CGCAGCTGCG TGCCCAGATG CGCGACCTGG CACGGGGCAT GTTCGTCTTT 
GGCTACGACA ACTACATGGC TCACGCCTTC CCCCAGGACG AGCTCAACCC CATCCACTGC 
CGCGGCCGTG GGCCCGACCG CGGGGACCCT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055489.1
CDS	100..2073
Translation	MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPVGRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	NM_014674.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014674.3

ORF Insert Sequence:

ATGCAATGGC GAGCGCTCGT CCTGGGGCTG GTGCTCCTCC GGCTTGGCCT CCATGGAGTA 
TTGTGGCTCG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGCTT TCCGCTCAGC 
TTCGGCTTCC AGCGTCTGAG GAGCCCCGAC GGCCCCGCGT CGCCCACCTC GGGGCCCGTG 
GGCCGGCCTG GGGGGGTATC CGGGCCGTCG TGGCTGCAGC CGCCGGGGAC CGGGGCAGCG 
CAGAGCCCGC GCAAGGCTCC GCGGCGTCCT GGGCCGGGGA TGTGCGGCCC AGCCAACTGG 
GGCTACGTGC TGGGCGGCCG GGGCCGCGGC CCGGACGAGT ACGAGAAGCG CTACAGCGGC 
GCCTTCCCTC CGCAGCTGCG TGCCCAGATG CGCGACCTGG CACGGGGCAT GTTCGTCTTT 
GGCTACGACA ACTACATGGC TCACGCCTTC CCCCAGGACG AGCTCAACCC CATCCACTGC 
CGCGGCCGTG GGCCCGACCG CGGGGACCCT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDEM1 ( XM_011534271.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532573.1 Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu63164
Clone ID Related Accession (Same CDS sequence)	XM_011534271.2 , XM_011534271.3 , XM_011534272.2
Accession Version	XM_011534271.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCTC TGTGCCTACA CCACACCAGT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532573.1
CDS	807..2300
Translation	MLSLCLHHTSSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	XM_011534271.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534271.2

ORF Insert Sequence:

ATGTTATCTC TGTGCCTACA CCACACCAGT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDEM1 ( XM_011534271.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532573.1 Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu63164
Clone ID Related Accession (Same CDS sequence)	XM_011534271.2 , XM_011534271.3 , XM_011534272.2
Accession Version	XM_011534271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534271.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCTC TGTGCCTACA CCACACCAGT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532573.1
CDS	907..2400
Translation	MLSLCLHHTSSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	XM_011534271.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534271.3

ORF Insert Sequence:

ATGTTATCTC TGTGCCTACA CCACACCAGT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDEM1 ( XM_011534272.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532574.1 Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu63164
Clone ID Related Accession (Same CDS sequence)	XM_011534271.2 , XM_011534271.3 , XM_011534272.2
Accession Version	XM_011534272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCTC TGTGCCTACA CCACACCAGT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532574.1
CDS	606..2099
Translation	MLSLCLHHTSSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	XM_011534272.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534272.2

ORF Insert Sequence:

ATGTTATCTC TGTGCCTACA CCACACCAGT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDEM1 ( NM_014674.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055489.1 Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), mRNA.

Price & Availability↑

CloneID	OHu15926
Clone ID Related Accession (Same CDS sequence)	NM_014674.3 , NM_014674.2
Accession Version	NM_014674.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC019088.1, AK292643.1, AC026202.7 and CN364698.1. On Aug 27, 2008 this sequence version replaced NM_014674.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86967.1, SRR1803612.130328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCAATGGC GAGCGCTCGT CCTGGGGCTG GTGCTCCTCC GGCTTGGCCT CCATGGAGTA 
TTGTGGCTCG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGCTT TCCGCTCAGC 
TTCGGCTTCC AGCGTCTGAG GAGCCCCGAC GGCCCCGCGT CGCCCACCTC GGGGCCCGTG 
GGCCGGCCTG GGGGGGTATC CGGGCCGTCG TGGCTGCAGC CGCCGGGGAC CGGGGCAGCG 
CAGAGCCCGC GCAAGGCTCC GCGGCGTCCT GGGCCGGGGA TGTGCGGCCC AGCCAACTGG 
GGCTACGTGC TGGGCGGCCG GGGCCGCGGC CCGGACGAGT ACGAGAAGCG CTACAGCGGC 
GCCTTCCCTC CGCAGCTGCG TGCCCAGATG CGCGACCTGG CACGGGGCAT GTTCGTCTTT 
GGCTACGACA ACTACATGGC TCACGCCTTC CCCCAGGACG AGCTCAACCC CATCCACTGC 
CGCGGCCGTG GGCCCGACCG CGGGGACCCT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055489.1
CDS	133..2106
Translation	MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPVGRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	NM_014674.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014674.2

ORF Insert Sequence:

ATGCAATGGC GAGCGCTCGT CCTGGGGCTG GTGCTCCTCC GGCTTGGCCT CCATGGAGTA 
TTGTGGCTCG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGCTT TCCGCTCAGC 
TTCGGCTTCC AGCGTCTGAG GAGCCCCGAC GGCCCCGCGT CGCCCACCTC GGGGCCCGTG 
GGCCGGCCTG GGGGGGTATC CGGGCCGTCG TGGCTGCAGC CGCCGGGGAC CGGGGCAGCG 
CAGAGCCCGC GCAAGGCTCC GCGGCGTCCT GGGCCGGGGA TGTGCGGCCC AGCCAACTGG 
GGCTACGTGC TGGGCGGCCG GGGCCGCGGC CCGGACGAGT ACGAGAAGCG CTACAGCGGC 
GCCTTCCCTC CGCAGCTGCG TGCCCAGATG CGCGACCTGG CACGGGGCAT GTTCGTCTTT 
GGCTACGACA ACTACATGGC TCACGCCTTC CCCCAGGACG AGCTCAACCC CATCCACTGC 
CGCGGCCGTG GGCCCGACCG CGGGGACCCT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAATAATGGG AAATTCATCC 
GAGTTCCAGA AAGCCGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC GATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACTGACT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATACATGG CCCATGACCT GGCGGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATTCCAT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTTG GGATTCTGAG TCGACTCCTG 
GGGGACTCCA CATTTGAGTG GGTGGCCAGA CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CAGGATTACT AGGCAATGTC GTGAACATTC AGACGGGCCA CTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GAGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC TCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTGCAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ACGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA TTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCATGAGTTA 
AAAGTCATCA ACTCCAGCTC CAACTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGTTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endoplasmic reticulum degradation-enhancing ?-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation.
Arthritis & rheumatology (Hoboken, N.J.)66(11)2976-88(2014 Nov)
Guiliano DB,Fussell H,Lenart I,Tsao E,Nesbeth D,Fletcher AJ,Campbell EC,Yousaf N,Williams S,Santos S,Cameron A,Towers GJ,Kellam P,Hebert DN,Gould KG,Powis SJ,Antoniou AN

MicroRNA-581 promotes hepatitis B virus surface antigen expression by targeting Dicer and EDEM1.
Carcinogenesis35(9)2127-33(2014 Sep)
Wang YQ,Ren YF,Song YJ,Xue YF,Zhang XJ,Cao ST,Deng ZJ,Wu J,Chen L,Li G,Shi KQ,Chen YP,Ren H,Huang AL,Tang KF

EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.
The Journal of cell biology206(3)347-56(2014 Aug)
Ninagawa S,Okada T,Sumitomo Y,Kamiya Y,Kato K,Horimoto S,Ishikawa T,Takeda S,Sakuma T,Yamamoto T,Mori K

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

EDEM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム