Edem1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Edem1
Entrez Gene ID	192193
Full Name	ER degradation enhancer, mannosidase alpha-like 1
Synonyms	A130059K23Rik,EDEM,mKIAA0212
General protein information	Preferred Names ER degradation enhancer, mannosidase alpha-like 1 Names ER degradation-enhancing alpha-mannosidase-like protein 1 ER degradation ER degradation enhancer, mannosidase alpha-like 1 ER degradation-enhancing alpha-mannosidase-like 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_138677.2	NP_619618.1	ER degradation-enhancing alpha-mannosidase-like protein 1
XM_006505746.3	XP_006505809.1	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
XM_006505746.4	XP_006505809.1	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Edem1	XP_238366.4
	EDEM1	XP_001098603.1
Canis lupus familiaris (dog)	EDEM1	XP_533753.2
Bos taurus (cattle)	EDEM1	NP_001096562.1
Mus musculus (house mouse)	Edem1	NP_619618.1
Gallus gallus (chicken)	EDEM1	NP_001006143.1
Homo sapiens (human)	EDEM1	NP_055489.1
Danio rerio (zebrafish)	edem1	NP_957483.1
Arabidopsis thaliana (thale cress)	AT1G27520	NP_564288.1
Oryza sativa (rice)	Os02g0741300	NP_001048080.1
Xenopus tropicalis (tropical clawed frog)	edem1	NP_989397.1
Pan troglodytes (chimpanzee)	EDEM1	XP_003309640.1
Caenorhabditis elegans (roundworm)	C47E12.3	NP_501802.2
Schizosaccharomyces pombe (fission yeast)	mnl1	NP_594434.1

Gene Ontology
Bibliography

Related articles in PubMed

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line.
Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, K?hn R, Rajewsky K
Proceedings of the National Academy of Sciences of the United States of America113(44)12514-12519(2016 Nov)

Neuronal Rap1 Regulates Energy Balance, Glucose Homeostasis, and Leptin Actions.
Kaneko K, Xu P, Cordonier EL, Chen SS, Ng A, Xu Y, Morozov A, Fukuda M
Cell reports16(11)3003-3015(2016 09)

The 78-kD Glucose-Regulated Protein Regulates Endoplasmic Reticulum Homeostasis and Distal Epithelial Cell Survival during Lung Development.
Flodby P, Li C, Liu Y, Wang H, Marconett CN, Laird-Offringa IA, Minoo P, Lee AS, Zhou B
American journal of respiratory cell and molecular biology55(1)135-49(2016 Jul)

Neural tube opening and abnormal extraembryonic membrane development in SEC23A deficient mice.
Zhu M, Tao J, Vasievich MP, Wei W, Zhu G, Khoriaty RN, Zhang B
Scientific reports515471(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes
Title: Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes.

Data suggest Edem1/Edem2 participate in ricin translocation; overexpression of Edem2, in contrast to Edem1, promotes ricin subunit A transport from endoplasmic reticulum (ER) to cytosol; transport of ricin P250A mutant is Edem1/Edem2-independent.
Title: The role of EDEM2 compared with EDEM1?in ricin transport from the endoplasmic reticulum to the cytosol.

analysis of a shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins
Title: A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins.

Cells with defective EDEM1 turnover contain unphysiologically high levels of EDEM1, show enhanced ER associated degradation activity and are characterized by impaired capacity to efficiently complete maturation of model glycopolypeptides.
Title: Segregation and rapid turnover of EDEM1 by an autophagy-like mechanism modulates standard ERAD and folding activities.

EDEM is involved in retrotranslocation of ricin from the ER to the cytosol.
Title: EDEM is involved in retrotranslocation of ricin from the endoplasmic reticulum to the cytosol.

The following Edem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15045	NM_138677.2 Latest version!	Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
OMu37016	XM_006505746.4 Latest version!	Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu37016	XM_006505746.3	Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Edem1 ( NM_138677.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619618.1 Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), mRNA.

Price & Availability↑

CloneID	OMu15045
Clone ID Related Accession (Same CDS sequence)	NM_138677.2
Accession Version	NM_138677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB619976.1, AB042828.1 and AC132443.3. On Dec 21, 2006 this sequence version replaced NM_138677.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB042828.1, AK129090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCAATGGC GAGCGCTTGT CCTGGGGCTG GTGCTGCTGC GCCTCGGCCT CCACGCGGTG 
CTCTGGTTGG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGGTT TCCGCTCAGC 
TTCGGCTTCC AGCGCTTGAG GGACCCCGAC GGCTCGGGGC CAGTTGGTCC GCCCGGAGGC 
CCGGCCTGGC TTCATCGGCC AAGGCGCGGA ACGGAGGGGC GGCTTGAGAC CCCGCCAGAG 
CCGGGACCGA CGCCGGGACC GGGCGTGTGC GGCCCGGCGC ACTGGGGCTA TGCCCTGGGC 
GGCGGTGGCT GCGGCCCGGA CGAGTACGAG CGGCGCTACA GCGGCGCCTT CCCGCCGCAG 
CTGCGTGCCC AGATGCGCGA CCTGGCGCGG GGCATGTTCG TCTTCGGCTA TGACAACTAC 
ATGGCGCACG CCTTTCCGCA GGACGAGCTC AACCCCATCT ACTGCCGCGG CCGGGGGCCT 
GACCGCGGAG ACCCTTCCAA TCTGAACATC AATGATGTCC TAGGGAATTA CTCTCTGACT 
CTGGTTGATG CCTTGGATAC ATTGGCAATA ATGGGAAATT CATCCGAGTT CCAGAAGGCA 
GTCAAGTTAG TGATCAACAC TGTTTCATTT GACAAAGATT CCACAGTCCA GGTCTTCGAA 
GCTACGATAA GGGTTCTGGG AAGCCTCCTT TCTGCTCACA GAATAATAAC TGATTCCAAA 
CAGCCCTTTG GTGACATGAC AATTGAGGAT TATGATAATG AATTGTTGTA CATGGCCCAT 
GACTTGGCTG TGCGGCTCCT TCCAGCCTTT GAAAACACCA AGACAGGGAT CCCCTATCCT 
CGGGTGAATC TGAAGACAGG TGTTCCTCCT GACAGCAATA ATGAGACCTG CACAGCGGGG 
GCCGGTTCTC TCCTGGTGGA ATTTGGGATT CTGAGCCGAC TGCTGGGGGA TTCCACTTTT 
GAGTGGGTTG CCAGACGAGC TGTGAAAGCC CTCTGGAACT TGCGGAGCAA CGATACAGGA 
TTATTAGGCA ATGTTGTGAA CATCCAGACA GGCCATTGGG TTGGAAAGCA GAGTGGCTTG 
GGTGCTGGGC TGGATTCCTT CTATGAGTAC CTCTTGAAAT CTTACATTCT TTTTGGAGAA 
AAAGAAGATC TAGAGATGTT TAATGCTGCG TATCAGAGCA TCCAGAGCTA CCTGCGAAGA 
GGCCGGGAAG CCTGCAATGA AGGAGAAGGA GACCCACCGC TCTACGTCAA CGTGAACATG 
TTCAGTGGGC AGCTCATGAA CACCTGGATT GACTCGCTGC AGGCTTTCTT CCCTGGACTG 
CAGGTTCTGA TAGGGGATGT GGAAGATGCC ATCTGCCTAC ACGCCTTCTA CTATGCCATA 
TGGAAGCGGT ACGGGGCCCT CCCTGAGCGC TATAACTGGC AACTCCAGGC CCCTGATGTT 
CTCTTCTACC CTCTGAGACC AGAGCTAGTG GAGTCCACAT ATCTCCTCTA CCAGGCAACC 
AAGAATCCCT TCTACCTCCA TGTAGGAATG GACATTCTGC AGAGTCTTGA AAAATACACA 
AAAGTCAAAT GTGGATATGC TACGCTGCAT CACGTCATAG ACAAGTCTAA AGAAGACCGG 
ATGGAAAGCT TCTTTCTCAG CGAGACTTGC AAATACTTGT ATCTGCTATT TGATGAAGAG 
AATCCAGTAC ACAAATCTGG AACCAGATAC ATGTTTACAA CTGAGGGCCA TATCATATCT 
GTGGACAAAC GTCTTCGGGA ATTGCCTTGG AAGGAATTCT TCTCTGAAGA TGGGGAGCGG 
GACCAAGAGG AAAAGTTTGT ACACAGACCT AAGTCTCAGG AGCTCAGAGT CATTAACTCC 
AGTTCTAATT GTAATCGTGT TCCTGATGAG AGGAGATACT CCTTGCCCTT AAAGAGCATC 
TACATGCGCC AGATCGACCA GATGGTTGGC TTGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619618.1
CDS	100..2058
Translation	MQWRALVLGLVLLRLGLHAVLWLVFGLGPSMGFYQRFPLSFGFQRLRDPDGSGPVGPPGGPAWLHRPRRGTEGRLETPPEPGPTPGPGVCGPAHWGYALGGGGCGPDEYERRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIYCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIEDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDSNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQSYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYMFTTEGHIISVDKRLRELPWKEFFSEDGERDQEEKFVHRPKSQELRVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	NM_138677.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138677.2

ORF Insert Sequence:

ATGCAATGGC GAGCGCTTGT CCTGGGGCTG GTGCTGCTGC GCCTCGGCCT CCACGCGGTG 
CTCTGGTTGG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGGTT TCCGCTCAGC 
TTCGGCTTCC AGCGCTTGAG GGACCCCGAC GGCTCGGGGC CAGTTGGTCC GCCCGGAGGC 
CCGGCCTGGC TTCATCGGCC AAGGCGCGGA ACGGAGGGGC GGCTTGAGAC CCCGCCAGAG 
CCGGGACCGA CGCCGGGACC GGGCGTGTGC GGCCCGGCGC ACTGGGGCTA TGCCCTGGGC 
GGCGGTGGCT GCGGCCCGGA CGAGTACGAG CGGCGCTACA GCGGCGCCTT CCCGCCGCAG 
CTGCGTGCCC AGATGCGCGA CCTGGCGCGG GGCATGTTCG TCTTCGGCTA TGACAACTAC 
ATGGCGCACG CCTTTCCGCA GGACGAGCTC AACCCCATCT ACTGCCGCGG CCGGGGGCCT 
GACCGCGGAG ACCCTTCCAA TCTGAACATC AATGATGTCC TAGGGAATTA CTCTCTGACT 
CTGGTTGATG CCTTGGATAC ATTGGCAATA ATGGGAAATT CATCCGAGTT CCAGAAGGCA 
GTCAAGTTAG TGATCAACAC TGTTTCATTT GACAAAGATT CCACAGTCCA GGTCTTCGAA 
GCTACGATAA GGGTTCTGGG AAGCCTCCTT TCTGCTCACA GAATAATAAC TGATTCCAAA 
CAGCCCTTTG GTGACATGAC AATTGAGGAT TATGATAATG AATTGTTGTA CATGGCCCAT 
GACTTGGCTG TGCGGCTCCT TCCAGCCTTT GAAAACACCA AGACAGGGAT CCCCTATCCT 
CGGGTGAATC TGAAGACAGG TGTTCCTCCT GACAGCAATA ATGAGACCTG CACAGCGGGG 
GCCGGTTCTC TCCTGGTGGA ATTTGGGATT CTGAGCCGAC TGCTGGGGGA TTCCACTTTT 
GAGTGGGTTG CCAGACGAGC TGTGAAAGCC CTCTGGAACT TGCGGAGCAA CGATACAGGA 
TTATTAGGCA ATGTTGTGAA CATCCAGACA GGCCATTGGG TTGGAAAGCA GAGTGGCTTG 
GGTGCTGGGC TGGATTCCTT CTATGAGTAC CTCTTGAAAT CTTACATTCT TTTTGGAGAA 
AAAGAAGATC TAGAGATGTT TAATGCTGCG TATCAGAGCA TCCAGAGCTA CCTGCGAAGA 
GGCCGGGAAG CCTGCAATGA AGGAGAAGGA GACCCACCGC TCTACGTCAA CGTGAACATG 
TTCAGTGGGC AGCTCATGAA CACCTGGATT GACTCGCTGC AGGCTTTCTT CCCTGGACTG 
CAGGTTCTGA TAGGGGATGT GGAAGATGCC ATCTGCCTAC ACGCCTTCTA CTATGCCATA 
TGGAAGCGGT ACGGGGCCCT CCCTGAGCGC TATAACTGGC AACTCCAGGC CCCTGATGTT 
CTCTTCTACC CTCTGAGACC AGAGCTAGTG GAGTCCACAT ATCTCCTCTA CCAGGCAACC 
AAGAATCCCT TCTACCTCCA TGTAGGAATG GACATTCTGC AGAGTCTTGA AAAATACACA 
AAAGTCAAAT GTGGATATGC TACGCTGCAT CACGTCATAG ACAAGTCTAA AGAAGACCGG 
ATGGAAAGCT TCTTTCTCAG CGAGACTTGC AAATACTTGT ATCTGCTATT TGATGAAGAG 
AATCCAGTAC ACAAATCTGG AACCAGATAC ATGTTTACAA CTGAGGGCCA TATCATATCT 
GTGGACAAAC GTCTTCGGGA ATTGCCTTGG AAGGAATTCT TCTCTGAAGA TGGGGAGCGG 
GACCAAGAGG AAAAGTTTGT ACACAGACCT AAGTCTCAGG AGCTCAGAGT CATTAACTCC 
AGTTCTAATT GTAATCGTGT TCCTGATGAG AGGAGATACT CCTTGCCCTT AAAGAGCATC 
TACATGCGCC AGATCGACCA GATGGTTGGC TTGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem1 ( XM_006505746.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505809.1 Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37016
Clone ID Related Accession (Same CDS sequence)	XM_006505746.3 , XM_006505746.4
Accession Version	XM_006505746.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATCCGAGTT CCAGAAGGCA GTCAAGTTAG TGATCAACAC TGTTTCATTT 
GACAAAGATT CCACAGTCCA GGTCTTCGAA GCTACGATAA GGGTTCTGGG AAGCCTCCTT 
TCTGCTCACA GAATAATAAC TGATTCCAAA CAGCCCTTTG GTGACATGAC AATTGAGGAT 
TATGATAATG AATTGTTGTA CATGGCCCAT GACTTGGCTG TGCGGCTCCT TCCAGCCTTT 
GAAAACACCA AGACAGGGAT CCCCTATCCT CGGGTGAATC TGAAGACAGG TGTTCCTCCT 
GACAGCAATA ATGAGACCTG CACAGCGGGG GCCGGTTCTC TCCTGGTGGA ATTTGGGATT 
CTGAGCCGAC TGCTGGGGGA TTCCACTTTT GAGTGGGTTG CCAGACGAGC TGTGAAAGCC 
CTCTGGAACT TGCGGAGCAA CGATACAGGA TTATTAGGCA ATGTTGTGAA CATCCAGACA 
GGCCATTGGG TTGGAAAGCA GAGTGGCTTG GGTGCTGGGC TGGATTCCTT CTATGAGTAC 
CTCTTGAAAT CTTACATTCT TTTTGGAGAA AAAGAAGATC TAGAGATGTT TAATGCTGCG 
TATCAGAGCA TCCAGAGCTA CCTGCGAAGA GGCCGGGAAG CCTGCAATGA AGGAGAAGGA 
GACCCACCGC TCTACGTCAA CGTGAACATG TTCAGTGGGC AGCTCATGAA CACCTGGATT 
GACTCGCTGC AGGCTTTCTT CCCTGGACTG CAGGTTCTGA TAGGGGATGT GGAAGATGCC 
ATCTGCCTAC ACGCCTTCTA CTATGCCATA TGGAAGCGGT ACGGGGCCCT CCCTGAGCGC 
TATAACTGGC AACTCCAGGC CCCTGATGTT CTCTTCTACC CTCTGAGACC AGAGCTAGTG 
GAGTCCACAT ATCTCCTCTA CCAGGCAACC AAGAATCCCT TCTACCTCCA TGTAGGAATG 
GACATTCTGC AGAGTCTTGA AAAATACACA AAAGTCAAAT GTGGATATGC TACGCTGCAT 
CACGTCATAG ACAAGTCTAA AGAAGACCGG ATGGAAAGCT TCTTTCTCAG CGAGACTTGC 
AAATACTTGT ATCTGCTATT TGATGAAGAG AATCCAGTAC ACAAATCTGG AACCAGATAC 
ATGTTTACAA CTGAGGGCCA TATCATATCT GTGGACAAAC GTCTTCGGGA ATTGCCTTGG 
AAGGAATTCT TCTCTGAAGA TGGGGAGCGG GACCAAGAGG AAAAGTTTGT ACACAGACCT 
AAGTCTCAGG AGCTCAGAGT CATTAACTCC AGTTCTAATT GTAATCGTGT TCCTGATGAG 
AGGAGATACT CCTTGCCCTT AAAGAGCATC TACATGCGCC AGATCGACCA GATGGTTGGC 
TTGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505809.1
CDS	173..1561
Translation	MGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIEDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDSNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQSYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYMFTTEGHIISVDKRLRELPWKEFFSEDGERDQEEKFVHRPKSQELRVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	XM_006505746.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505746.3

ORF Insert Sequence:

ATGGGAAATT CATCCGAGTT CCAGAAGGCA GTCAAGTTAG TGATCAACAC TGTTTCATTT 
GACAAAGATT CCACAGTCCA GGTCTTCGAA GCTACGATAA GGGTTCTGGG AAGCCTCCTT 
TCTGCTCACA GAATAATAAC TGATTCCAAA CAGCCCTTTG GTGACATGAC AATTGAGGAT 
TATGATAATG AATTGTTGTA CATGGCCCAT GACTTGGCTG TGCGGCTCCT TCCAGCCTTT 
GAAAACACCA AGACAGGGAT CCCCTATCCT CGGGTGAATC TGAAGACAGG TGTTCCTCCT 
GACAGCAATA ATGAGACCTG CACAGCGGGG GCCGGTTCTC TCCTGGTGGA ATTTGGGATT 
CTGAGCCGAC TGCTGGGGGA TTCCACTTTT GAGTGGGTTG CCAGACGAGC TGTGAAAGCC 
CTCTGGAACT TGCGGAGCAA CGATACAGGA TTATTAGGCA ATGTTGTGAA CATCCAGACA 
GGCCATTGGG TTGGAAAGCA GAGTGGCTTG GGTGCTGGGC TGGATTCCTT CTATGAGTAC 
CTCTTGAAAT CTTACATTCT TTTTGGAGAA AAAGAAGATC TAGAGATGTT TAATGCTGCG 
TATCAGAGCA TCCAGAGCTA CCTGCGAAGA GGCCGGGAAG CCTGCAATGA AGGAGAAGGA 
GACCCACCGC TCTACGTCAA CGTGAACATG TTCAGTGGGC AGCTCATGAA CACCTGGATT 
GACTCGCTGC AGGCTTTCTT CCCTGGACTG CAGGTTCTGA TAGGGGATGT GGAAGATGCC 
ATCTGCCTAC ACGCCTTCTA CTATGCCATA TGGAAGCGGT ACGGGGCCCT CCCTGAGCGC 
TATAACTGGC AACTCCAGGC CCCTGATGTT CTCTTCTACC CTCTGAGACC AGAGCTAGTG 
GAGTCCACAT ATCTCCTCTA CCAGGCAACC AAGAATCCCT TCTACCTCCA TGTAGGAATG 
GACATTCTGC AGAGTCTTGA AAAATACACA AAAGTCAAAT GTGGATATGC TACGCTGCAT 
CACGTCATAG ACAAGTCTAA AGAAGACCGG ATGGAAAGCT TCTTTCTCAG CGAGACTTGC 
AAATACTTGT ATCTGCTATT TGATGAAGAG AATCCAGTAC ACAAATCTGG AACCAGATAC 
ATGTTTACAA CTGAGGGCCA TATCATATCT GTGGACAAAC GTCTTCGGGA ATTGCCTTGG 
AAGGAATTCT TCTCTGAAGA TGGGGAGCGG GACCAAGAGG AAAAGTTTGT ACACAGACCT 
AAGTCTCAGG AGCTCAGAGT CATTAACTCC AGTTCTAATT GTAATCGTGT TCCTGATGAG 
AGGAGATACT CCTTGCCCTT AAAGAGCATC TACATGCGCC AGATCGACCA GATGGTTGGC 
TTGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem1 ( XM_006505746.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505809.1 Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37016
Clone ID Related Accession (Same CDS sequence)	XM_006505746.3 , XM_006505746.4
Accession Version	XM_006505746.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006505746.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATCCGAGTT CCAGAAGGCA GTCAAGTTAG TGATCAACAC TGTTTCATTT 
GACAAAGATT CCACAGTCCA GGTCTTCGAA GCTACGATAA GGGTTCTGGG AAGCCTCCTT 
TCTGCTCACA GAATAATAAC TGATTCCAAA CAGCCCTTTG GTGACATGAC AATTGAGGAT 
TATGATAATG AATTGTTGTA CATGGCCCAT GACTTGGCTG TGCGGCTCCT TCCAGCCTTT 
GAAAACACCA AGACAGGGAT CCCCTATCCT CGGGTGAATC TGAAGACAGG TGTTCCTCCT 
GACAGCAATA ATGAGACCTG CACAGCGGGG GCCGGTTCTC TCCTGGTGGA ATTTGGGATT 
CTGAGCCGAC TGCTGGGGGA TTCCACTTTT GAGTGGGTTG CCAGACGAGC TGTGAAAGCC 
CTCTGGAACT TGCGGAGCAA CGATACAGGA TTATTAGGCA ATGTTGTGAA CATCCAGACA 
GGCCATTGGG TTGGAAAGCA GAGTGGCTTG GGTGCTGGGC TGGATTCCTT CTATGAGTAC 
CTCTTGAAAT CTTACATTCT TTTTGGAGAA AAAGAAGATC TAGAGATGTT TAATGCTGCG 
TATCAGAGCA TCCAGAGCTA CCTGCGAAGA GGCCGGGAAG CCTGCAATGA AGGAGAAGGA 
GACCCACCGC TCTACGTCAA CGTGAACATG TTCAGTGGGC AGCTCATGAA CACCTGGATT 
GACTCGCTGC AGGCTTTCTT CCCTGGACTG CAGGTTCTGA TAGGGGATGT GGAAGATGCC 
ATCTGCCTAC ACGCCTTCTA CTATGCCATA TGGAAGCGGT ACGGGGCCCT CCCTGAGCGC 
TATAACTGGC AACTCCAGGC CCCTGATGTT CTCTTCTACC CTCTGAGACC AGAGCTAGTG 
GAGTCCACAT ATCTCCTCTA CCAGGCAACC AAGAATCCCT TCTACCTCCA TGTAGGAATG 
GACATTCTGC AGAGTCTTGA AAAATACACA AAAGTCAAAT GTGGATATGC TACGCTGCAT 
CACGTCATAG ACAAGTCTAA AGAAGACCGG ATGGAAAGCT TCTTTCTCAG CGAGACTTGC 
AAATACTTGT ATCTGCTATT TGATGAAGAG AATCCAGTAC ACAAATCTGG AACCAGATAC 
ATGTTTACAA CTGAGGGCCA TATCATATCT GTGGACAAAC GTCTTCGGGA ATTGCCTTGG 
AAGGAATTCT TCTCTGAAGA TGGGGAGCGG GACCAAGAGG AAAAGTTTGT ACACAGACCT 
AAGTCTCAGG AGCTCAGAGT CATTAACTCC AGTTCTAATT GTAATCGTGT TCCTGATGAG 
AGGAGATACT CCTTGCCCTT AAAGAGCATC TACATGCGCC AGATCGACCA GATGGTTGGC 
TTGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505809.1
CDS	175..1563
Translation	MGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIEDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDSNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQSYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYMFTTEGHIISVDKRLRELPWKEFFSEDGERDQEEKFVHRPKSQELRVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	XM_006505746.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505746.4

ORF Insert Sequence:

ATGGGAAATT CATCCGAGTT CCAGAAGGCA GTCAAGTTAG TGATCAACAC TGTTTCATTT 
GACAAAGATT CCACAGTCCA GGTCTTCGAA GCTACGATAA GGGTTCTGGG AAGCCTCCTT 
TCTGCTCACA GAATAATAAC TGATTCCAAA CAGCCCTTTG GTGACATGAC AATTGAGGAT 
TATGATAATG AATTGTTGTA CATGGCCCAT GACTTGGCTG TGCGGCTCCT TCCAGCCTTT 
GAAAACACCA AGACAGGGAT CCCCTATCCT CGGGTGAATC TGAAGACAGG TGTTCCTCCT 
GACAGCAATA ATGAGACCTG CACAGCGGGG GCCGGTTCTC TCCTGGTGGA ATTTGGGATT 
CTGAGCCGAC TGCTGGGGGA TTCCACTTTT GAGTGGGTTG CCAGACGAGC TGTGAAAGCC 
CTCTGGAACT TGCGGAGCAA CGATACAGGA TTATTAGGCA ATGTTGTGAA CATCCAGACA 
GGCCATTGGG TTGGAAAGCA GAGTGGCTTG GGTGCTGGGC TGGATTCCTT CTATGAGTAC 
CTCTTGAAAT CTTACATTCT TTTTGGAGAA AAAGAAGATC TAGAGATGTT TAATGCTGCG 
TATCAGAGCA TCCAGAGCTA CCTGCGAAGA GGCCGGGAAG CCTGCAATGA AGGAGAAGGA 
GACCCACCGC TCTACGTCAA CGTGAACATG TTCAGTGGGC AGCTCATGAA CACCTGGATT 
GACTCGCTGC AGGCTTTCTT CCCTGGACTG CAGGTTCTGA TAGGGGATGT GGAAGATGCC 
ATCTGCCTAC ACGCCTTCTA CTATGCCATA TGGAAGCGGT ACGGGGCCCT CCCTGAGCGC 
TATAACTGGC AACTCCAGGC CCCTGATGTT CTCTTCTACC CTCTGAGACC AGAGCTAGTG 
GAGTCCACAT ATCTCCTCTA CCAGGCAACC AAGAATCCCT TCTACCTCCA TGTAGGAATG 
GACATTCTGC AGAGTCTTGA AAAATACACA AAAGTCAAAT GTGGATATGC TACGCTGCAT 
CACGTCATAG ACAAGTCTAA AGAAGACCGG ATGGAAAGCT TCTTTCTCAG CGAGACTTGC 
AAATACTTGT ATCTGCTATT TGATGAAGAG AATCCAGTAC ACAAATCTGG AACCAGATAC 
ATGTTTACAA CTGAGGGCCA TATCATATCT GTGGACAAAC GTCTTCGGGA ATTGCCTTGG 
AAGGAATTCT TCTCTGAAGA TGGGGAGCGG GACCAAGAGG AAAAGTTTGT ACACAGACCT 
AAGTCTCAGG AGCTCAGAGT CATTAACTCC AGTTCTAATT GTAATCGTGT TCCTGATGAG 
AGGAGATACT CCTTGCCCTT AAAGAGCATC TACATGCGCC AGATCGACCA GATGGTTGGC 
TTGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line.
Proceedings of the National Academy of Sciences of the United States of America113(44)12514-12519(2016 Nov)
Chu VT,Graf R,Wirtz T,Weber T,Favret J,Li X,Petsch K,Tran NT,Sieweke MH,Berek C,K?hn R,Rajewsky K

Neuronal Rap1 Regulates Energy Balance, Glucose Homeostasis, and Leptin Actions.
Cell reports16(11)3003-3015(2016 09)
Kaneko K,Xu P,Cordonier EL,Chen SS,Ng A,Xu Y,Morozov A,Fukuda M

The 78-kD Glucose-Regulated Protein Regulates Endoplasmic Reticulum Homeostasis and Distal Epithelial Cell Survival during Lung Development.
American journal of respiratory cell and molecular biology55(1)135-49(2016 Jul)
Flodby P,Li C,Liu Y,Wang H,Marconett CN,Laird-Offringa IA,Minoo P,Lee AS,Zhou B

Neural tube opening and abnormal extraembryonic membrane development in SEC23A deficient mice.
Scientific reports515471(2015 Oct)
Zhu M,Tao J,Vasievich MP,Wei W,Zhu G,Khoriaty RN,Zhang B

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Edem1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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