PIR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIR
Entrez Gene ID	8544
Full Name	pirin
General protein information	Preferred Names pirin Names pirin pirin (iron-binding nuclear protein) probable quercetin 2,3-dioxygenase PIR probable quercetinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.2
Summary	This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	300931

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018109.3	NP_001018119.1	pirin
NM_003662.3	NP_003653.1	pirin
NM_001018109.2	NP_001018119.1	pirin

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIR	NP_003653.1
Arabidopsis thaliana (thale cress)	AT2G43120	NP_850385.1
Bos taurus (cattle)	PIR	NP_001095828.1
Mus musculus (house mouse)	Pir	NP_081429.1
Danio rerio (zebrafish)	pir	NP_001002550.1
Kluyveromyces lactis	KLLA0D01243g	XP_453124.1
Oryza sativa (rice)	Os03g0845000	NP_001051876.1
Rattus norvegicus (Norway rat)	Pir	NP_001009474.1
Arabidopsis thaliana (thale cress)	PRN	NP_191481.1
	PIR	XP_002806203.1
Canis lupus familiaris (dog)	PIR	XP_005641188.1
Arabidopsis thaliana (thale cress)	AT3G59260	NP_191485.1
Pan troglodytes (chimpanzee)	PIR	XP_003317418.2
Magnaporthe oryzae (rice blast fungus)	MGG_02400	XP_003709181.1
Neurospora crassa	NCU03369	XP_956304.1
Xenopus tropicalis (tropical clawed frog)	pir	NP_001011440.1

Gene Ontology
Bibliography

Related articles in PubMed

Redox-Specific Allosteric Modulation of the Conformational Dynamics of ?B DNA by Pirin in the NF-?B Supramolecular Complex.
Adeniran C, Hamelberg D
Biochemistry56(37)5002-5010(2017 09)

Pirin regulates epithelial to mesenchymal transition independently of Bcl3-Slug signaling.
Komai K, Niwa Y, Sasazawa Y, Simizu S
FEBS letters589(6)738-43(2015 Mar)

Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element.
Brz?ska K, St?pkowski TM, Kruszewski M
Molecular and cellular biochemistry389(1-2)99-111(2014 Apr)

Pirin down-regulates the EAF2/U19 protein and alleviates its growth inhibition in prostate cancer cells.
Qiao Z, Wang D, Hahn J, Ai J, Wang Z
The Prostate74(2)113-20(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that only the Fe(III) form of pirin [not the Fe(II) form] enhances affinity of binding between p65 and DNA in the pirin-p65-DNA supramolecular complex. (pirin = quercetin 2,3-dioxygenase; p65 = RELA proto-oncogene NF-kappaB subunit)
Title: Redox-Specific Allosteric Modulation of the Conformational Dynamics of ?B DNA by Pirin in the NF-?B Supramolecular Complex.

Pirin regulates E-cadherin independently of Bcl3-Slug signaling.
Title: Pirin regulates epithelial to mesenchymal transition independently of Bcl3-Slug signaling.

The NRF2 transcription factor binds to this element in vivo and drives the basal PIR expression.
Title: Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element.

Pirin is a newly identified binding partner of EAF2/U19 capable of down-regulating EAF2/U19 protein and alleviating its inhibition of prostate cancer cell survival/proliferation.
Title: Pirin down-regulates the EAF2/U19 protein and alleviates its growth inhibition in prostate cancer cells.

Data indicate that the R-shaped area composes the interface for pirin-NF-kappaB binding that is responsible for modulation of NF-kappaB's DNA-binding properties.
Title: Pirin is an iron-dependent redox regulator of NF-?B.

The following PIR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11599	NM_003662.3 Latest version!	Homo sapiens pirin (PIR), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu11599	NM_001018109.3 Latest version!	Homo sapiens pirin (PIR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu11599	NM_001018109.2	Homo sapiens pirin (PIR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIR ( NM_001018109.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018119.1 Homo sapiens pirin (PIR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11599
Clone ID Related Accession (Same CDS sequence)	NM_001018109.3 , NM_003662.3 , NM_001018109.2
Accession Version	NM_001018109.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	pirin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA686501.1, AC097625.11 and BC002517.2. On Nov 22, 2018 this sequence version replaced NM_001018109.2. Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y07867.1, SRR1803614.105885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380420.10/ ENSP00000369785.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGTCCT CCAAGAAAGT TACTCTCTCA GTGCTCAGCC GGGAGCAGTC GGAAGGGGTT 
GGAGCGAGGG TCCGGAGAAG CATTGGCAGA CCCGAGTTAA AAAATCTGGA TCCGTTTTTA 
CTGTTTGATG AATTTAAAGG AGGTAGACCA GGAGGATTTC CTGATCATCC ACATCGAGGT 
TTTGAAACAG TATCCTACCT CCTGGAAGGG GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CACACTGGTA AAATGAACCC AGGAGATTTG CAGTGGATGA CTGCGGGCCG GGGCATTCTG 
CACGCTGAGA TGCCTTGCTC AGAGGAGCCA GCCCATGGCC TACAACTGTG GGTTAATTTG 
AGGAGCTCAG AGAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAAATCCCT 
AAACCCAGTA AGGATGGTGT GACAGTTGCT GTCATTTCTG GAGAAGCCCT GGGAATAAAG 
TCCAAGGTTT ACACTCGCAC ACCAACCTTA TATTTGGACT TCAAATTGGA CCCAGGAGCC 
AAACATTCCC AACCTATCCC TAAAGGGTGG ACAAGCTTCA TTTACACGAT ATCTGGAGAT 
GTGTATATTG GGCCCGATGA TGCACAACAA AAAATAGAAC CTCATCACAC AGCAGTGCTT 
GGAGAAGGTG ACAGTGTCCA GGTGGAGAAC AAGGATCCCA AGAGAAGCCA CTTTGTCTTA 
ATTGCTGGGG AGCCATTAAG AGAACCAGTT ATCCAACATG GTCCATTTGT GATGAACACC 
AATGAAGAGA TTTCTCAAGC TATTCTTGAT TTCAGAAACG CAAAAAATGG GTTTGAAAGG 
GCCAAAACCT GGAAATCAAA GATTGGGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018119.1
CDS	197..1069
Translation	MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTWKSKIGN

Target ORF information:

RefSeq Version	NM_001018109.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pirin (PIR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018109.3

ORF Insert Sequence:

ATGGGGTCCT CCAAGAAAGT TACTCTCTCA GTGCTCAGCC GGGAGCAGTC GGAAGGGGTT 
GGAGCGAGGG TCCGGAGAAG CATTGGCAGA CCCGAGTTAA AAAATCTGGA TCCGTTTTTA 
CTGTTTGATG AATTTAAAGG AGGTAGACCA GGAGGATTTC CTGATCATCC ACATCGAGGT 
TTTGAAACAG TATCCTACCT CCTGGAAGGG GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CACACTGGTA AAATGAACCC AGGAGATTTG CAGTGGATGA CTGCGGGCCG GGGCATTCTG 
CACGCTGAGA TGCCTTGCTC AGAGGAGCCA GCCCATGGCC TACAACTGTG GGTTAATTTG 
AGGAGCTCAG AGAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAAATCCCT 
AAACCCAGTA AGGATGGTGT GACAGTTGCT GTCATTTCTG GAGAAGCCCT GGGAATAAAG 
TCCAAGGTTT ACACTCGCAC ACCAACCTTA TATTTGGACT TCAAATTGGA CCCAGGAGCC 
AAACATTCCC AACCTATCCC TAAAGGGTGG ACAAGCTTCA TTTACACGAT ATCTGGAGAT 
GTGTATATTG GGCCCGATGA TGCACAACAA AAAATAGAAC CTCATCACAC AGCAGTGCTT 
GGAGAAGGTG ACAGTGTCCA GGTGGAGAAC AAGGATCCCA AGAGAAGCCA CTTTGTCTTA 
ATTGCTGGGG AGCCATTAAG AGAACCAGTT ATCCAACATG GTCCATTTGT GATGAACACC 
AATGAAGAGA TTTCTCAAGC TATTCTTGAT TTCAGAAACG CAAAAAATGG GTTTGAAAGG 
GCCAAAACCT GGAAATCAAA GATTGGGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIR ( NM_003662.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003653.1 Homo sapiens pirin (PIR), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11599
Clone ID Related Accession (Same CDS sequence)	NM_001018109.3 , NM_003662.3 , NM_001018109.2
Accession Version	NM_003662.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	pirin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA686501.1, DT217415.1, Y07868.1 and BC002517.2. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2011 this sequence version replaced NM_003662.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y07868.1, SRR1163655.335638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGTCCT CCAAGAAAGT TACTCTCTCA GTGCTCAGCC GGGAGCAGTC GGAAGGGGTT 
GGAGCGAGGG TCCGGAGAAG CATTGGCAGA CCCGAGTTAA AAAATCTGGA TCCGTTTTTA 
CTGTTTGATG AATTTAAAGG AGGTAGACCA GGAGGATTTC CTGATCATCC ACATCGAGGT 
TTTGAAACAG TATCCTACCT CCTGGAAGGG GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CACACTGGTA AAATGAACCC AGGAGATTTG CAGTGGATGA CTGCGGGCCG GGGCATTCTG 
CACGCTGAGA TGCCTTGCTC AGAGGAGCCA GCCCATGGCC TACAACTGTG GGTTAATTTG 
AGGAGCTCAG AGAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAAATCCCT 
AAACCCAGTA AGGATGGTGT GACAGTTGCT GTCATTTCTG GAGAAGCCCT GGGAATAAAG 
TCCAAGGTTT ACACTCGCAC ACCAACCTTA TATTTGGACT TCAAATTGGA CCCAGGAGCC 
AAACATTCCC AACCTATCCC TAAAGGGTGG ACAAGCTTCA TTTACACGAT ATCTGGAGAT 
GTGTATATTG GGCCCGATGA TGCACAACAA AAAATAGAAC CTCATCACAC AGCAGTGCTT 
GGAGAAGGTG ACAGTGTCCA GGTGGAGAAC AAGGATCCCA AGAGAAGCCA CTTTGTCTTA 
ATTGCTGGGG AGCCATTAAG AGAACCAGTT ATCCAACATG GTCCATTTGT GATGAACACC 
AATGAAGAGA TTTCTCAAGC TATTCTTGAT TTCAGAAACG CAAAAAATGG GTTTGAAAGG 
GCCAAAACCT GGAAATCAAA GATTGGGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003653.1
CDS	486..1358
Translation	MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTWKSKIGN

Target ORF information:

RefSeq Version	NM_003662.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pirin (PIR), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003662.3

ORF Insert Sequence:

ATGGGGTCCT CCAAGAAAGT TACTCTCTCA GTGCTCAGCC GGGAGCAGTC GGAAGGGGTT 
GGAGCGAGGG TCCGGAGAAG CATTGGCAGA CCCGAGTTAA AAAATCTGGA TCCGTTTTTA 
CTGTTTGATG AATTTAAAGG AGGTAGACCA GGAGGATTTC CTGATCATCC ACATCGAGGT 
TTTGAAACAG TATCCTACCT CCTGGAAGGG GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CACACTGGTA AAATGAACCC AGGAGATTTG CAGTGGATGA CTGCGGGCCG GGGCATTCTG 
CACGCTGAGA TGCCTTGCTC AGAGGAGCCA GCCCATGGCC TACAACTGTG GGTTAATTTG 
AGGAGCTCAG AGAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAAATCCCT 
AAACCCAGTA AGGATGGTGT GACAGTTGCT GTCATTTCTG GAGAAGCCCT GGGAATAAAG 
TCCAAGGTTT ACACTCGCAC ACCAACCTTA TATTTGGACT TCAAATTGGA CCCAGGAGCC 
AAACATTCCC AACCTATCCC TAAAGGGTGG ACAAGCTTCA TTTACACGAT ATCTGGAGAT 
GTGTATATTG GGCCCGATGA TGCACAACAA AAAATAGAAC CTCATCACAC AGCAGTGCTT 
GGAGAAGGTG ACAGTGTCCA GGTGGAGAAC AAGGATCCCA AGAGAAGCCA CTTTGTCTTA 
ATTGCTGGGG AGCCATTAAG AGAACCAGTT ATCCAACATG GTCCATTTGT GATGAACACC 
AATGAAGAGA TTTCTCAAGC TATTCTTGAT TTCAGAAACG CAAAAAATGG GTTTGAAAGG 
GCCAAAACCT GGAAATCAAA GATTGGGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIR ( NM_001018109.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018119.1 Homo sapiens pirin (PIR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11599
Clone ID Related Accession (Same CDS sequence)	NM_001018109.3 , NM_003662.3 , NM_001018109.2
Accession Version	NM_001018109.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	pirin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA686501.1, AC097625.11 and BC002517.2. On Feb 12, 2011 this sequence version replaced NM_001018109.1. Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y07867.1, SRR1803614.105885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGTCCT CCAAGAAAGT TACTCTCTCA GTGCTCAGCC GGGAGCAGTC GGAAGGGGTT 
GGAGCGAGGG TCCGGAGAAG CATTGGCAGA CCCGAGTTAA AAAATCTGGA TCCGTTTTTA 
CTGTTTGATG AATTTAAAGG AGGTAGACCA GGAGGATTTC CTGATCATCC ACATCGAGGT 
TTTGAAACAG TATCCTACCT CCTGGAAGGG GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CACACTGGTA AAATGAACCC AGGAGATTTG CAGTGGATGA CTGCGGGCCG GGGCATTCTG 
CACGCTGAGA TGCCTTGCTC AGAGGAGCCA GCCCATGGCC TACAACTGTG GGTTAATTTG 
AGGAGCTCAG AGAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAAATCCCT 
AAACCCAGTA AGGATGGTGT GACAGTTGCT GTCATTTCTG GAGAAGCCCT GGGAATAAAG 
TCCAAGGTTT ACACTCGCAC ACCAACCTTA TATTTGGACT TCAAATTGGA CCCAGGAGCC 
AAACATTCCC AACCTATCCC TAAAGGGTGG ACAAGCTTCA TTTACACGAT ATCTGGAGAT 
GTGTATATTG GGCCCGATGA TGCACAACAA AAAATAGAAC CTCATCACAC AGCAGTGCTT 
GGAGAAGGTG ACAGTGTCCA GGTGGAGAAC AAGGATCCCA AGAGAAGCCA CTTTGTCTTA 
ATTGCTGGGG AGCCATTAAG AGAACCAGTT ATCCAACATG GTCCATTTGT GATGAACACC 
AATGAAGAGA TTTCTCAAGC TATTCTTGAT TTCAGAAACG CAAAAAATGG GTTTGAAAGG 
GCCAAAACCT GGAAATCAAA GATTGGGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018119.1
CDS	452..1324
Translation	MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTWKSKIGN

Target ORF information:

RefSeq Version	NM_001018109.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pirin (PIR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018109.2

ORF Insert Sequence:

ATGGGGTCCT CCAAGAAAGT TACTCTCTCA GTGCTCAGCC GGGAGCAGTC GGAAGGGGTT 
GGAGCGAGGG TCCGGAGAAG CATTGGCAGA CCCGAGTTAA AAAATCTGGA TCCGTTTTTA 
CTGTTTGATG AATTTAAAGG AGGTAGACCA GGAGGATTTC CTGATCATCC ACATCGAGGT 
TTTGAAACAG TATCCTACCT CCTGGAAGGG GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CACACTGGTA AAATGAACCC AGGAGATTTG CAGTGGATGA CTGCGGGCCG GGGCATTCTG 
CACGCTGAGA TGCCTTGCTC AGAGGAGCCA GCCCATGGCC TACAACTGTG GGTTAATTTG 
AGGAGCTCAG AGAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAAATCCCT 
AAACCCAGTA AGGATGGTGT GACAGTTGCT GTCATTTCTG GAGAAGCCCT GGGAATAAAG 
TCCAAGGTTT ACACTCGCAC ACCAACCTTA TATTTGGACT TCAAATTGGA CCCAGGAGCC 
AAACATTCCC AACCTATCCC TAAAGGGTGG ACAAGCTTCA TTTACACGAT ATCTGGAGAT 
GTGTATATTG GGCCCGATGA TGCACAACAA AAAATAGAAC CTCATCACAC AGCAGTGCTT 
GGAGAAGGTG ACAGTGTCCA GGTGGAGAAC AAGGATCCCA AGAGAAGCCA CTTTGTCTTA 
ATTGCTGGGG AGCCATTAAG AGAACCAGTT ATCCAACATG GTCCATTTGT GATGAACACC 
AATGAAGAGA TTTCTCAAGC TATTCTTGAT TTCAGAAACG CAAAAAATGG GTTTGAAAGG 
GCCAAAACCT GGAAATCAAA GATTGGGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Redox-Specific Allosteric Modulation of the Conformational Dynamics of ?B DNA by Pirin in the NF-?B Supramolecular Complex.
Biochemistry56(37)5002-5010(2017 09)
Adeniran C,Hamelberg D

Pirin regulates epithelial to mesenchymal transition independently of Bcl3-Slug signaling.
FEBS letters589(6)738-43(2015 Mar)
Komai K,Niwa Y,Sasazawa Y,Simizu S

Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element.
Molecular and cellular biochemistry389(1-2)99-111(2014 Apr)
Brz?ska K,St?pkowski TM,Kruszewski M

Pirin down-regulates the EAF2/U19 protein and alleviates its growth inhibition in prostate cancer cells.
The Prostate74(2)113-20(2014 Feb)
Qiao Z,Wang D,Hahn J,Ai J,Wang Z