PPFIA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPFIA1
Entrez Gene ID	8500
Full Name	PTPRF interacting protein alpha 1
Synonyms	LIP.1,LIP1,LIPRIN
General protein information	Preferred Names PTPRF interacting protein alpha 1 Names liprin-alpha-1 LAR-interacting protein 1 LIP-1 Liprin-alpha1 protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.3
Summary	The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	611054

mRNA and Protein(s)

mRNA	Protein	Name
NM_003626.3	NP_003617.1	liprin-alpha-1 isoform b
XM_011545308.3	XP_011543610.1	liprin-alpha-1 isoform X3
XM_011545315.2	XP_011543617.1	liprin-alpha-1 isoform X11
XM_017018449.1	XP_016873938.1	liprin-alpha-1 isoform X14
XM_017018450.1	XP_016873939.1	liprin-alpha-1 isoform X15
XM_011545319.1	XP_011543621.1	liprin-alpha-1 isoform X17
XM_011545306.2	XP_011543608.1	liprin-alpha-1 isoform X1
XM_011545310.2	XP_011543612.1	liprin-alpha-1 isoform X5
XM_011545306.3	XP_011543608.1	liprin-alpha-1 isoform X1
XM_011545307.3	XP_011543609.1	liprin-alpha-1 isoform X2
XM_011545310.3	XP_011543612.1	liprin-alpha-1 isoform X5
XM_011545311.3	XP_011543613.1	liprin-alpha-1 isoform X6
XM_011545317.2	XP_011543619.1	liprin-alpha-1 isoform X13
XM_011545318.3	XP_011543620.1	liprin-alpha-1 isoform X16
XM_011545308.2	XP_011543610.1	liprin-alpha-1 isoform X3
NM_003626.4	NP_003617.1	liprin-alpha-1 isoform b
XM_011545313.2	XP_011543615.1	liprin-alpha-1 isoform X8
XM_017018448.1	XP_016873937.1	liprin-alpha-1 isoform X9
XM_011545316.2	XP_011543618.1	liprin-alpha-1 isoform X12
NM_177423.2	NP_803172.1	liprin-alpha-1 isoform a
XM_011545307.2	XP_011543609.1	liprin-alpha-1 isoform X2
XM_011545311.2	XP_011543613.1	liprin-alpha-1 isoform X6
XM_011545318.2	XP_011543620.1	liprin-alpha-1 isoform X16
XM_006718716.3	XP_006718779.1	liprin-alpha-1 isoform X1
XM_011545309.2	XP_011543611.1	liprin-alpha-1 isoform X4
XM_011545312.2	XP_011543614.1	liprin-alpha-1 isoform X7
XM_011545314.3	XP_011543616.1	liprin-alpha-1 isoform X10
XM_011545314.2	XP_011543616.1	liprin-alpha-1 isoform X10

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPFIA1	NP_003617.1
Pan troglodytes (chimpanzee)	PPFIA1	XP_003952009.1
Gallus gallus (chicken)	PPFIA1	XP_421074.4
Danio rerio (zebrafish)	zgc:171298	NP_001104714.1
Xenopus tropicalis (tropical clawed frog)	ppfia1	NP_001121469.1
Mus musculus (house mouse)	Ppfia1	NP_001182015.1
Bos taurus (cattle)	PPFIA1	NP_001178306.1
Rattus norvegicus (Norway rat)	Ppfia1	NP_001099790.2
Canis lupus familiaris (dog)	PPFIA1	XP_005631430.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a membrane-less compartment regulating invadosome function and motility.
Sala K, Raimondi A, Tonoli D, Tacchetti C, de Curtis I
Scientific reports8(1)1164(2018 Jan)

PPFIA1 is upregulated in liver metastasis of breast cancer and is a potential poor prognostic indicator of metastatic relapse.
Yang J, Wu NN, Huang DJ, Luo YC, Huang JZ, He HY, Lu HL, Song WL
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(7)1010428317713492(2017 Jul)

PPFIA1 drives active ?5?1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
Mana G, Clapero F, Panieri E, Panero V, B?ttcher RT, Tseng HY, Saltarin F, Astanina E, Wolanska KI, Morgan MR, Humphries MJ, Santoro MM, Serini G, Valdembri D
Nature communications713546(2016 Nov)

Liprin-?1 is a regulator of vimentin intermediate filament network in the cancer cell adhesion machinery.
Pehkonen H, von Nandelstadh P, Karhemo PR, Lepikhova T, Grenman R, Lehti K, Monni O
Scientific reports624486(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Based on these findings, we infer that high PPFIA1 expression might be an independent prognostic indicator of increased metastatic relapse risk in patients with estrogen receptor+/N- breast cancer, but not in estrogen receptor+/N+ or estrogen receptor- patients.
Title: PPFIA1 is upregulated in liver metastasis of breast cancer and is a potential poor prognostic indicator of metastatic relapse.

These results indicate that liprin-alpha1 localizes to different adhesion and cytoskeletal structures to regulate vimentin intermediate filament network, thereby altering the invasion and growth properties of the cancer cells.
Title: Liprin-?1 is a regulator of vimentin intermediate filament network in the cancer cell adhesion machinery.

The human tumor cell line MDA-MB-231 expresses liprin-alpha 1 and is able to promote the formation of metastasis in mice.
Title: Effects of the scaffold proteins liprin-?1, ?1 and ?2 on invasion by breast cancer cells.

results suggested that PPFIA1 functioned with PP2A to promote the dephosphorylation of Kif7, triggering Kif7 localization to the tips of primary cilia and promoting Gli transcriptional activity.
Title: The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling.

Liprin-alpha1, ERC1a and LL5 also define new highly polarized and dynamic cytoplasmic structures uniquely localized near the protruding cell edge
Title: Liprin-?1, ERC1 and LL5 define polarized and dynamic structures that are implicated in cell migration.

The following PPFIA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPFIA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu61872	XM_011545318.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu92602	XM_017018448.1 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92603	XM_017018449.1 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92604	XM_017018450.1 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu55873	NM_177423.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$832.30 $1189.00
OHu55875	NM_003626.4 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu45568	XM_011545306.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu45568	XM_006718716.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61861	XM_011545307.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61862	XM_011545308.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61863	XM_011545309.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61864	XM_011545310.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61865	XM_011545311.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61866	XM_011545312.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61867	XM_011545313.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61868	XM_011545314.3 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61869	XM_011545315.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61870	XM_011545316.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61871	XM_011545317.2 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61873	XM_011545319.1 Latest version!	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu45568	XM_011545306.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61864	XM_011545310.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61862	XM_011545308.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu55875	NM_003626.3	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu61861	XM_011545307.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61865	XM_011545311.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61872	XM_011545318.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61868	XM_011545314.2	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPFIA1 ( XM_011545318.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543620.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu61872
Clone ID Related Accession (Same CDS sequence)	XM_011545318.3 , XM_011545318.2
Accession Version	XM_011545318.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC AGGAGCAGCT GGACGCCATC AACAAAGAGA TCAGGTTGAT TCAGGAAGAA 
AAAGAAAATA CAGAGCAGCG GGCAGAGGAG ATTGAAAGTC GAGTTGGCAG TGGAAGTCTA 
GACAATCTTG GTCGTTTTAG ATCAATGAGC TCCATTCCCC CCTACCCTGC TTCCTCGCTT 
GCTAGCTCCT CCCCTCCGGG CAGTGGGCGC TCCACCCCAC GAAGGATCCC TCACAGCCCA 
GCTCGGGAAG TGGACAGACT GGGCGTCATG ACCCTTCCTA GTGATTTAAG GAAACATCGT 
AGAAAGTTGC CACCTTCCAG AGAAGAGGTA CGAGATGACA AGACAACCAT AAAGTGTGAA 
ACCTCCCCGC CTTCCTCCCC GAGAGCCCTT CGGTTAGACC GGCTGCACAA AGGGGCGCTG 
CACACCGTCA GCCACGAGGA CATCAGGGAC ATAAGGAACT CCACAGGCTC CCAGGATGGT 
CCCGTGAGCA ACCCCAGCAG TAGCAACAGT AGCCAGGACT CGCTCCACAA AGCCCCAAAG 
AAGAAAGGCA TTAAGTCCTC CATTGGCCGC TTGTTTGGCA AGAAAGAAAA GGGCCGACCT 
GGACAAACTG GCAAAGAAGC ATTAGGACAA GCTGGTGTTT CCGAGACGGA TAACTCATCT 
CAGGATGCCT TGGGACTTAG CAAATTGGGG GGACAGGCTG AAAAAAATCG TAAACTTCAA 
AAAAAGCATG AATTGCTGGA GGAAGCCCGG AGACAAGGTT TACCTTTTGC CCAATGGGAC 
GGGCCAACGG TTGTGGTCTG GCTAGAGCTC TGGGTTGGGA TGCCAGCCTG GTATGTGGCT 
GCCTGCCGAG CAAACGTGAA AAGCGGGGCC ATCATGTCGG CCCTGTCCGA CACAGAGATC 
CAGCGTGAGA TTGGCATCAG CAACCCCCTG CACAGGCTGA AGCTGAGGCT GGCCATCCAG 
GAGATCATGT CGCTGACCAG CCCGTCTGCC CCGCCCACAT CTAGAACGAC CACAGGAAAT 
GTCTGGTTAA CACACGAAGA GATGGAAACG CTCGCAGCCA CGCCGCAAAC GGAAGATGAG 
GAGGGAAGCT GGGCTCAGAC ACTCGCCTAT GGGGACATGA ACCACGAGTG GATCGGCAAC 
GAGTGGCTCC CCAGCCTGGG CCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTTGTA 
GACGCCAGGA TGCTGGACCA CTTGACCAAG AAAGACCTTC GAGGGCAGCT GAAAATGGTC 
GACAGTTTTC ACAGAAACAG TTTCCAGTGT GGAATTATGT GCCTGAGAAG GTTAAATTAT 
GACCGGAAAG AACTGGAAAG AAAAAGAGAA GAAAGTCAGA GTGAAATAAA AGACGTGCTT 
GTTTGGAGCA ATGATCGAGT GATTCGCTGG ATCCTGTCAA TTGGCCTTAA AGAATATGCA 
AACAATCTTA TAGAGAGTGG TGTTCACGGA GCACTTCTGG CCTTAGATGA AACCTTCGAC 
TTCAGTGCAC TGGCACTGCT GTTACAGATC CCGACGCAGA ACACACAGGC TCGTGCTGTC 
TTGGAAAGAG AATTTAACAA CCTTTTGGTC ATGGGGACTG ATAGAAGGTT TGATGAAGAT 
GATGATAAAA GCTTTAGGAG AGCACCTTCA TGGAGAAAAA AGTTTAGACC AAAGGACATT 
CGTGGCTTAG CTGCTGGGTC AGCAGAGACT CTCCCTGCAA ACTTCCGGGT GACTTCTTCT 
ATGTCTTCCC CCTCTATGCA GCCAAAGAAG ATGCAGATGG ACGGCAATGT ATCAGGAACA 
CAGAGGTTGG ATTCTGCTAC AGTCAGGACT TACTCCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543620.1
CDS	413..2254
Translation	MMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545318.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545318.3

ORF Insert Sequence:

ATGATGCTTC AGGAGCAGCT GGACGCCATC AACAAAGAGA TCAGGTTGAT TCAGGAAGAA 
AAAGAAAATA CAGAGCAGCG GGCAGAGGAG ATTGAAAGTC GAGTTGGCAG TGGAAGTCTA 
GACAATCTTG GTCGTTTTAG ATCAATGAGC TCCATTCCCC CCTACCCTGC TTCCTCGCTT 
GCTAGCTCCT CCCCTCCGGG CAGTGGGCGC TCCACCCCAC GAAGGATCCC TCACAGCCCA 
GCTCGGGAAG TGGACAGACT GGGCGTCATG ACCCTTCCTA GTGATTTAAG GAAACATCGT 
AGAAAGTTGC CACCTTCCAG AGAAGAGGTA CGAGATGACA AGACAACCAT AAAGTGTGAA 
ACCTCCCCGC CTTCCTCCCC GAGAGCCCTT CGGTTAGACC GGCTGCACAA AGGGGCGCTG 
CACACCGTCA GCCACGAGGA CATCAGGGAC ATAAGGAACT CCACAGGCTC CCAGGATGGT 
CCCGTGAGCA ACCCCAGCAG TAGCAACAGT AGCCAGGACT CGCTCCACAA AGCCCCAAAG 
AAGAAAGGCA TTAAGTCCTC CATTGGCCGC TTGTTTGGCA AGAAAGAAAA GGGCCGACCT 
GGACAAACTG GCAAAGAAGC ATTAGGACAA GCTGGTGTTT CCGAGACGGA TAACTCATCT 
CAGGATGCCT TGGGACTTAG CAAATTGGGG GGACAGGCTG AAAAAAATCG TAAACTTCAA 
AAAAAGCATG AATTGCTGGA GGAAGCCCGG AGACAAGGTT TACCTTTTGC CCAATGGGAC 
GGGCCAACGG TTGTGGTCTG GCTAGAGCTC TGGGTTGGGA TGCCAGCCTG GTATGTGGCT 
GCCTGCCGAG CAAACGTGAA AAGCGGGGCC ATCATGTCGG CCCTGTCCGA CACAGAGATC 
CAGCGTGAGA TTGGCATCAG CAACCCCCTG CACAGGCTGA AGCTGAGGCT GGCCATCCAG 
GAGATCATGT CGCTGACCAG CCCGTCTGCC CCGCCCACAT CTAGAACGAC CACAGGAAAT 
GTCTGGTTAA CACACGAAGA GATGGAAACG CTCGCAGCCA CGCCGCAAAC GGAAGATGAG 
GAGGGAAGCT GGGCTCAGAC ACTCGCCTAT GGGGACATGA ACCACGAGTG GATCGGCAAC 
GAGTGGCTCC CCAGCCTGGG CCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTTGTA 
GACGCCAGGA TGCTGGACCA CTTGACCAAG AAAGACCTTC GAGGGCAGCT GAAAATGGTC 
GACAGTTTTC ACAGAAACAG TTTCCAGTGT GGAATTATGT GCCTGAGAAG GTTAAATTAT 
GACCGGAAAG AACTGGAAAG AAAAAGAGAA GAAAGTCAGA GTGAAATAAA AGACGTGCTT 
GTTTGGAGCA ATGATCGAGT GATTCGCTGG ATCCTGTCAA TTGGCCTTAA AGAATATGCA 
AACAATCTTA TAGAGAGTGG TGTTCACGGA GCACTTCTGG CCTTAGATGA AACCTTCGAC 
TTCAGTGCAC TGGCACTGCT GTTACAGATC CCGACGCAGA ACACACAGGC TCGTGCTGTC 
TTGGAAAGAG AATTTAACAA CCTTTTGGTC ATGGGGACTG ATAGAAGGTT TGATGAAGAT 
GATGATAAAA GCTTTAGGAG AGCACCTTCA TGGAGAAAAA AGTTTAGACC AAAGGACATT 
CGTGGCTTAG CTGCTGGGTC AGCAGAGACT CTCCCTGCAA ACTTCCGGGT GACTTCTTCT 
ATGTCTTCCC CCTCTATGCA GCCAAAGAAG ATGCAGATGG ACGGCAATGT ATCAGGAACA 
CAGAGGTTGG ATTCTGCTAC AGTCAGGACT TACTCCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545318.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543620.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu61872
Clone ID Related Accession (Same CDS sequence)	XM_011545318.3 , XM_011545318.2
Accession Version	XM_011545318.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC AGGAGCAGCT GGACGCCATC AACAAAGAGA TCAGGTTGAT TCAGGAAGAA 
AAAGAAAATA CAGAGCAGCG GGCAGAGGAG ATTGAAAGTC GAGTTGGCAG TGGAAGTCTA 
GACAATCTTG GTCGTTTTAG ATCAATGAGC TCCATTCCCC CCTACCCTGC TTCCTCGCTT 
GCTAGCTCCT CCCCTCCGGG CAGTGGGCGC TCCACCCCAC GAAGGATCCC TCACAGCCCA 
GCTCGGGAAG TGGACAGACT GGGCGTCATG ACCCTTCCTA GTGATTTAAG GAAACATCGT 
AGAAAGTTGC CACCTTCCAG AGAAGAGGTA CGAGATGACA AGACAACCAT AAAGTGTGAA 
ACCTCCCCGC CTTCCTCCCC GAGAGCCCTT CGGTTAGACC GGCTGCACAA AGGGGCGCTG 
CACACCGTCA GCCACGAGGA CATCAGGGAC ATAAGGAACT CCACAGGCTC CCAGGATGGT 
CCCGTGAGCA ACCCCAGCAG TAGCAACAGT AGCCAGGACT CGCTCCACAA AGCCCCAAAG 
AAGAAAGGCA TTAAGTCCTC CATTGGCCGC TTGTTTGGCA AGAAAGAAAA GGGCCGACCT 
GGACAAACTG GCAAAGAAGC ATTAGGACAA GCTGGTGTTT CCGAGACGGA TAACTCATCT 
CAGGATGCCT TGGGACTTAG CAAATTGGGG GGACAGGCTG AAAAAAATCG TAAACTTCAA 
AAAAAGCATG AATTGCTGGA GGAAGCCCGG AGACAAGGTT TACCTTTTGC CCAATGGGAC 
GGGCCAACGG TTGTGGTCTG GCTAGAGCTC TGGGTTGGGA TGCCAGCCTG GTATGTGGCT 
GCCTGCCGAG CAAACGTGAA AAGCGGGGCC ATCATGTCGG CCCTGTCCGA CACAGAGATC 
CAGCGTGAGA TTGGCATCAG CAACCCCCTG CACAGGCTGA AGCTGAGGCT GGCCATCCAG 
GAGATCATGT CGCTGACCAG CCCGTCTGCC CCGCCCACAT CTAGAACGAC CACAGGAAAT 
GTCTGGTTAA CACACGAAGA GATGGAAACG CTCGCAGCCA CGCCGCAAAC GGAAGATGAG 
GAGGGAAGCT GGGCTCAGAC ACTCGCCTAT GGGGACATGA ACCACGAGTG GATCGGCAAC 
GAGTGGCTCC CCAGCCTGGG CCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTTGTA 
GACGCCAGGA TGCTGGACCA CTTGACCAAG AAAGACCTTC GAGGGCAGCT GAAAATGGTC 
GACAGTTTTC ACAGAAACAG TTTCCAGTGT GGAATTATGT GCCTGAGAAG GTTAAATTAT 
GACCGGAAAG AACTGGAAAG AAAAAGAGAA GAAAGTCAGA GTGAAATAAA AGACGTGCTT 
GTTTGGAGCA ATGATCGAGT GATTCGCTGG ATCCTGTCAA TTGGCCTTAA AGAATATGCA 
AACAATCTTA TAGAGAGTGG TGTTCACGGA GCACTTCTGG CCTTAGATGA AACCTTCGAC 
TTCAGTGCAC TGGCACTGCT GTTACAGATC CCGACGCAGA ACACACAGGC TCGTGCTGTC 
TTGGAAAGAG AATTTAACAA CCTTTTGGTC ATGGGGACTG ATAGAAGGTT TGATGAAGAT 
GATGATAAAA GCTTTAGGAG AGCACCTTCA TGGAGAAAAA AGTTTAGACC AAAGGACATT 
CGTGGCTTAG CTGCTGGGTC AGCAGAGACT CTCCCTGCAA ACTTCCGGGT GACTTCTTCT 
ATGTCTTCCC CCTCTATGCA GCCAAAGAAG ATGCAGATGG ACGGCAATGT ATCAGGAACA 
CAGAGGTTGG ATTCTGCTAC AGTCAGGACT TACTCCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543620.1
CDS	413..2254
Translation	MMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545318.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545318.2

ORF Insert Sequence:

ATGATGCTTC AGGAGCAGCT GGACGCCATC AACAAAGAGA TCAGGTTGAT TCAGGAAGAA 
AAAGAAAATA CAGAGCAGCG GGCAGAGGAG ATTGAAAGTC GAGTTGGCAG TGGAAGTCTA 
GACAATCTTG GTCGTTTTAG ATCAATGAGC TCCATTCCCC CCTACCCTGC TTCCTCGCTT 
GCTAGCTCCT CCCCTCCGGG CAGTGGGCGC TCCACCCCAC GAAGGATCCC TCACAGCCCA 
GCTCGGGAAG TGGACAGACT GGGCGTCATG ACCCTTCCTA GTGATTTAAG GAAACATCGT 
AGAAAGTTGC CACCTTCCAG AGAAGAGGTA CGAGATGACA AGACAACCAT AAAGTGTGAA 
ACCTCCCCGC CTTCCTCCCC GAGAGCCCTT CGGTTAGACC GGCTGCACAA AGGGGCGCTG 
CACACCGTCA GCCACGAGGA CATCAGGGAC ATAAGGAACT CCACAGGCTC CCAGGATGGT 
CCCGTGAGCA ACCCCAGCAG TAGCAACAGT AGCCAGGACT CGCTCCACAA AGCCCCAAAG 
AAGAAAGGCA TTAAGTCCTC CATTGGCCGC TTGTTTGGCA AGAAAGAAAA GGGCCGACCT 
GGACAAACTG GCAAAGAAGC ATTAGGACAA GCTGGTGTTT CCGAGACGGA TAACTCATCT 
CAGGATGCCT TGGGACTTAG CAAATTGGGG GGACAGGCTG AAAAAAATCG TAAACTTCAA 
AAAAAGCATG AATTGCTGGA GGAAGCCCGG AGACAAGGTT TACCTTTTGC CCAATGGGAC 
GGGCCAACGG TTGTGGTCTG GCTAGAGCTC TGGGTTGGGA TGCCAGCCTG GTATGTGGCT 
GCCTGCCGAG CAAACGTGAA AAGCGGGGCC ATCATGTCGG CCCTGTCCGA CACAGAGATC 
CAGCGTGAGA TTGGCATCAG CAACCCCCTG CACAGGCTGA AGCTGAGGCT GGCCATCCAG 
GAGATCATGT CGCTGACCAG CCCGTCTGCC CCGCCCACAT CTAGAACGAC CACAGGAAAT 
GTCTGGTTAA CACACGAAGA GATGGAAACG CTCGCAGCCA CGCCGCAAAC GGAAGATGAG 
GAGGGAAGCT GGGCTCAGAC ACTCGCCTAT GGGGACATGA ACCACGAGTG GATCGGCAAC 
GAGTGGCTCC CCAGCCTGGG CCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTTGTA 
GACGCCAGGA TGCTGGACCA CTTGACCAAG AAAGACCTTC GAGGGCAGCT GAAAATGGTC 
GACAGTTTTC ACAGAAACAG TTTCCAGTGT GGAATTATGT GCCTGAGAAG GTTAAATTAT 
GACCGGAAAG AACTGGAAAG AAAAAGAGAA GAAAGTCAGA GTGAAATAAA AGACGTGCTT 
GTTTGGAGCA ATGATCGAGT GATTCGCTGG ATCCTGTCAA TTGGCCTTAA AGAATATGCA 
AACAATCTTA TAGAGAGTGG TGTTCACGGA GCACTTCTGG CCTTAGATGA AACCTTCGAC 
TTCAGTGCAC TGGCACTGCT GTTACAGATC CCGACGCAGA ACACACAGGC TCGTGCTGTC 
TTGGAAAGAG AATTTAACAA CCTTTTGGTC ATGGGGACTG ATAGAAGGTT TGATGAAGAT 
GATGATAAAA GCTTTAGGAG AGCACCTTCA TGGAGAAAAA AGTTTAGACC AAAGGACATT 
CGTGGCTTAG CTGCTGGGTC AGCAGAGACT CTCCCTGCAA ACTTCCGGGT GACTTCTTCT 
ATGTCTTCCC CCTCTATGCA GCCAAAGAAG ATGCAGATGG ACGGCAATGT ATCAGGAACA 
CAGAGGTTGG ATTCTGCTAC AGTCAGGACT TACTCCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_017018448.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873937.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu92602
Clone ID Related Accession (Same CDS sequence)	XM_017018448.1
Accession Version	XM_017018448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGTTGCC ACCTTCCAGA GAAGAGGTAC GAGATGACAA GACAACCATA 
AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG AGAGCCCTTC GGTTAGACCG GCTGCACAAA 
GGGGCGCTGC ACACCGTCAG CCACGAGGAC ATCAGGGACA TAAGGAACTC CACAGGCTCC 
CAGGATGGTC CCGTGAGCAA CCCCAGCAGT AGCAACAGTA GCCAGGACTC GCTCCACAAA 
GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG 
GGCCGACCTG GACAAACTGG CAAAGAAGCA TTAGGACAAG CTGGTGTTTC CGAGACGGAT 
AACTCATCTC AGGATGCCTT GGGACTTAGC AAATTGGGGG GACAGGCTGA AAAAAATCGT 
AAACTTCAAA AAAAGCATGA ATTGCTGGAG GAAGCCCGGA GACAAGGTTT ACCTTTTGCC 
CAATGGGACG GGCCAACGGT TGTGGTCTGG CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG 
TATGTGGCTG CCTGCCGAGC AAACGTGAAA AGCGGGGCCA TCATGTCGGC CCTGTCCGAC 
ACAGAGATCC AGCGTGAGAT TGGCATCAGC AACCCCCTGC ACAGGCTGAA GCTGAGGCTG 
GCCATCCAGG AGATCATGTC GCTGACCAGC CCGTCTGCCC CGCCCACATC TAGAACGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873937.1
CDS	229..4029
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_017018448.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018448.1

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGTTGCC ACCTTCCAGA GAAGAGGTAC GAGATGACAA GACAACCATA 
AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG AGAGCCCTTC GGTTAGACCG GCTGCACAAA 
GGGGCGCTGC ACACCGTCAG CCACGAGGAC ATCAGGGACA TAAGGAACTC CACAGGCTCC 
CAGGATGGTC CCGTGAGCAA CCCCAGCAGT AGCAACAGTA GCCAGGACTC GCTCCACAAA 
GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG 
GGCCGACCTG GACAAACTGG CAAAGAAGCA TTAGGACAAG CTGGTGTTTC CGAGACGGAT 
AACTCATCTC AGGATGCCTT GGGACTTAGC AAATTGGGGG GACAGGCTGA AAAAAATCGT 
AAACTTCAAA AAAAGCATGA ATTGCTGGAG GAAGCCCGGA GACAAGGTTT ACCTTTTGCC 
CAATGGGACG GGCCAACGGT TGTGGTCTGG CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG 
TATGTGGCTG CCTGCCGAGC AAACGTGAAA AGCGGGGCCA TCATGTCGGC CCTGTCCGAC 
ACAGAGATCC AGCGTGAGAT TGGCATCAGC AACCCCCTGC ACAGGCTGAA GCTGAGGCTG 
GCCATCCAGG AGATCATGTC GCTGACCAGC CCGTCTGCCC CGCCCACATC TAGAACGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_017018449.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873938.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu92603
Clone ID Related Accession (Same CDS sequence)	XM_017018449.1
Accession Version	XM_017018449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCTAGTG ATTTAAGGAA ACATCGTAGA AAGTTGCCAC CTTCCAGAGA AGAGGTACGA 
GATGACAAGA CAACCATAAA GTGTGAAACC TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG 
TTAGACCGGC TGCACAAAGG GGCGCTGCAC ACCGTCAGCC ACGAGGACAT CAGGGACATA 
AGGAACTCCA CAGGCTCCCA GGATGGTCCC GTGAGCAACC CCAGCAGTAG CAACAGTAGC 
CAGGACTCGC TCCACAAAGC CCCAAAGAAG AAAGGCATTA AGTCCTCCAT TGGCCGCTTG 
TTTGGCAAGA AAGAAAAGGG CCGACCTGGA CAAACTGGCA AAGAAGCATT AGGACAAGCT 
GGTGTTTCCG AGACGGATAA CTCATCTCAG GATGCCTTGG GACTTAGCAA ATTGGGGGGA 
CAGGCTGAAA AAAATCGTAA ACTTCAAAAA AAGCATGAAT TGCTGGAGGA AGCCCGGAGA 
CAAGGTTTAC CTTTTGCCCA ATGGGACGGG CCAACGGTTG TGGTCTGGCT AGAGCTCTGG 
GTTGGGATGC CAGCCTGGTA TGTGGCTGCC TGCCGAGCAA ACGTGAAAAG CGGGGCCATC 
ATGTCGGCCC TGTCCGACAC AGAGATCCAG CGTGAGATTG GCATCAGCAA CCCCCTGCAC 
AGGCTGAAGC TGAGGCTGGC CATCCAGGAG ATCATGTCGC TGACCAGCCC GTCTGCCCCG 
CCCACATCTA GAACGACACT CGCCTATGGG GACATGAACC ACGAGTGGAT CGGCAACGAG 
TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC CGCAGCTACT TCATGGAGTG CCTTGTAGAC 
GCCAGGATGC TGGACCACTT GACCAAGAAA GACCTTCGAG GGCAGCTGAA AATGGTCGAC 
AGTTTTCACA GAAACAGTTT CCAGTGTGGA ATTATGTGCC TGAGAAGGTT AAATTATGAC 
CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA AGTCAGAGTG AAATAAAAGA CGTGCTTGTT 
TGGAGCAATG ATCGAGTGAT TCGCTGGATC CTGTCAATTG GCCTTAAAGA ATATGCAAAC 
AATCTTATAG AGAGTGGTGT TCACGGAGCA CTTCTGGCCT TAGATGAAAC CTTCGACTTC 
AGTGCACTGG CACTGCTGTT ACAGATCCCG ACGCAGAACA CACAGGCTCG TGCTGTCTTG 
GAAAGAGAAT TTAACAACCT TTTGGTCATG GGGACTGATA GAAGGTTTGA TGAAGATGAT 
GATAAAAGCT TTAGGAGAGC ACCTTCATGG AGAAAAAAGT TTAGACCAAA GGACATTCGT 
GGCTTAGCTG CTGGGTCAGC AGAGACTCTC CCTGCAAACT TCCGGGTGAC TTCTTCTATG 
TCTTCCCCCT CTATGCAGCC AAAGAAGATG CAGATGGACG GCAATGTATC AGGAACACAG 
AGGTTGGATT CTGCTACAGT CAGGACTTAC TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873938.1
CDS	229..3867
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_017018449.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018449.1

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCTAGTG ATTTAAGGAA ACATCGTAGA AAGTTGCCAC CTTCCAGAGA AGAGGTACGA 
GATGACAAGA CAACCATAAA GTGTGAAACC TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG 
TTAGACCGGC TGCACAAAGG GGCGCTGCAC ACCGTCAGCC ACGAGGACAT CAGGGACATA 
AGGAACTCCA CAGGCTCCCA GGATGGTCCC GTGAGCAACC CCAGCAGTAG CAACAGTAGC 
CAGGACTCGC TCCACAAAGC CCCAAAGAAG AAAGGCATTA AGTCCTCCAT TGGCCGCTTG 
TTTGGCAAGA AAGAAAAGGG CCGACCTGGA CAAACTGGCA AAGAAGCATT AGGACAAGCT 
GGTGTTTCCG AGACGGATAA CTCATCTCAG GATGCCTTGG GACTTAGCAA ATTGGGGGGA 
CAGGCTGAAA AAAATCGTAA ACTTCAAAAA AAGCATGAAT TGCTGGAGGA AGCCCGGAGA 
CAAGGTTTAC CTTTTGCCCA ATGGGACGGG CCAACGGTTG TGGTCTGGCT AGAGCTCTGG 
GTTGGGATGC CAGCCTGGTA TGTGGCTGCC TGCCGAGCAA ACGTGAAAAG CGGGGCCATC 
ATGTCGGCCC TGTCCGACAC AGAGATCCAG CGTGAGATTG GCATCAGCAA CCCCCTGCAC 
AGGCTGAAGC TGAGGCTGGC CATCCAGGAG ATCATGTCGC TGACCAGCCC GTCTGCCCCG 
CCCACATCTA GAACGACACT CGCCTATGGG GACATGAACC ACGAGTGGAT CGGCAACGAG 
TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC CGCAGCTACT TCATGGAGTG CCTTGTAGAC 
GCCAGGATGC TGGACCACTT GACCAAGAAA GACCTTCGAG GGCAGCTGAA AATGGTCGAC 
AGTTTTCACA GAAACAGTTT CCAGTGTGGA ATTATGTGCC TGAGAAGGTT AAATTATGAC 
CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA AGTCAGAGTG AAATAAAAGA CGTGCTTGTT 
TGGAGCAATG ATCGAGTGAT TCGCTGGATC CTGTCAATTG GCCTTAAAGA ATATGCAAAC 
AATCTTATAG AGAGTGGTGT TCACGGAGCA CTTCTGGCCT TAGATGAAAC CTTCGACTTC 
AGTGCACTGG CACTGCTGTT ACAGATCCCG ACGCAGAACA CACAGGCTCG TGCTGTCTTG 
GAAAGAGAAT TTAACAACCT TTTGGTCATG GGGACTGATA GAAGGTTTGA TGAAGATGAT 
GATAAAAGCT TTAGGAGAGC ACCTTCATGG AGAAAAAAGT TTAGACCAAA GGACATTCGT 
GGCTTAGCTG CTGGGTCAGC AGAGACTCTC CCTGCAAACT TCCGGGTGAC TTCTTCTATG 
TCTTCCCCCT CTATGCAGCC AAAGAAGATG CAGATGGACG GCAATGTATC AGGAACACAG 
AGGTTGGATT CTGCTACAGT CAGGACTTAC TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_017018450.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873939.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X16, mRNA.

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CloneID	OHu92604
Clone ID Related Accession (Same CDS sequence)	XM_017018450.1
Accession Version	XM_017018450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGATG GGACTGCCAC CTGCTGCCAA GGGCAGTACA 
CAGTGGCCAG CTCTTAAACC AGGGAGAACT TTGAGCTGCG CCGAGAGACT AGGGGAGGAA 
GACATCAAGG CGTGGTCAAA ACCATTTATA CCTCATTTAA CAAAATTAGT AAGAGTGCTG 
GCTGCGTGGA GCATCTATGA GCAGGACACC CATGGTCCCC CCGTCATGGA GCTCCCATTT 
TATGGAGTTC ATAAGACTGG GTTGTACTTG TGGCTGTTTC AGTGCTGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873939.1
CDS	229..3582
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTMGLPPAAKGSTQWPALKPGRTLSCAERLGEEDIKAWSKPFIPHLTKLVRVLAAWSIYEQDTHGPPVMELPFYGVHKTGLYLWLFQCWE

Target ORF information:

RefSeq Version	XM_017018450.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018450.1

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGATG GGACTGCCAC CTGCTGCCAA GGGCAGTACA 
CAGTGGCCAG CTCTTAAACC AGGGAGAACT TTGAGCTGCG CCGAGAGACT AGGGGAGGAA 
GACATCAAGG CGTGGTCAAA ACCATTTATA CCTCATTTAA CAAAATTAGT AAGAGTGCTG 
GCTGCGTGGA GCATCTATGA GCAGGACACC CATGGTCCCC CCGTCATGGA GCTCCCATTT 
TATGGAGTTC ATAAGACTGG GTTGTACTTG TGGCTGTTTC AGTGCTGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( NM_177423.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_803172.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 1, mRNA.

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CloneID	OHu55873
Clone ID Related Accession (Same CDS sequence)	NM_177423.2
Accession Version	NM_177423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW163189.1, U22815.1 and BF352121.1. On Oct 14, 2011 this sequence version replaced NM_177423.1. Transcript Variant: This variant (1) differs in the 3' end, which includes a part of the coding region and 3' UTR, as compared to variant 2. The resulting protein (isoform a) has a distinct and shorter C-terminus, as compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U22815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTTTGCCAC CTTCCAGAGA AGAGGTACGA GATGACAAGA CAACCATAAA GTGTGAAACC 
TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG TTAGACCGGC TGCACAAAGG GGCGCTGCAC 
ACCGTCAGCC ACGAGGACAT CAGGGACATA AGGAACTCCA CAGGCTCCCA GGATGGTCCC 
GTGAGCAACC CCAGCAGTAG CAACAGTAGC CAGGACTCGC TCCACAAAGC CCCAAAGAAG 
AAAGGCATTA AGTCCTCCAT TGGCCGCTTG TTTGGCAAGA AAGAAAAGGG CCGACCTGGA 
CAAACTGGCA AAGAAGCATT AGGACAAGCT GGTGTTTCCG AGACGGATAA CTCATCTCAG 
GATGCCTTGG GACTTAGCAA ATTGGGGGGA CAGGCTGAAA AAAATCGTAA ACTTCAAAAA 
AAGCATGAAT TGCTGGAGGA AGCCCGGAGA CAAGGTTTAC CTTTTGCCCA ATGGGACGGG 
CCAACGGTTG TGGTCTGGCT AGAGCTCTGG GTTGGGATGC CAGCCTGGTA TGTGGCTGCC 
TGCCGAGCAA ACGTGAAAAG CGGGGCCATC ATGTCGGCCC TGTCCGACAC AGAGATCCAG 
CGTGAGATTG GCATCAGCAA CCCCCTGCAC AGGCTGAAGC TGAGGCTGGC CATCCAGGAG 
ATCATGTCGC TGACCAGCCC GTCTGCCCCG CCCACATCTA GAACGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803172.1
CDS	216..3773
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGM

Target ORF information:

RefSeq Version	NM_177423.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177423.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTTTGCCAC CTTCCAGAGA AGAGGTACGA GATGACAAGA CAACCATAAA GTGTGAAACC 
TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG TTAGACCGGC TGCACAAAGG GGCGCTGCAC 
ACCGTCAGCC ACGAGGACAT CAGGGACATA AGGAACTCCA CAGGCTCCCA GGATGGTCCC 
GTGAGCAACC CCAGCAGTAG CAACAGTAGC CAGGACTCGC TCCACAAAGC CCCAAAGAAG 
AAAGGCATTA AGTCCTCCAT TGGCCGCTTG TTTGGCAAGA AAGAAAAGGG CCGACCTGGA 
CAAACTGGCA AAGAAGCATT AGGACAAGCT GGTGTTTCCG AGACGGATAA CTCATCTCAG 
GATGCCTTGG GACTTAGCAA ATTGGGGGGA CAGGCTGAAA AAAATCGTAA ACTTCAAAAA 
AAGCATGAAT TGCTGGAGGA AGCCCGGAGA CAAGGTTTAC CTTTTGCCCA ATGGGACGGG 
CCAACGGTTG TGGTCTGGCT AGAGCTCTGG GTTGGGATGC CAGCCTGGTA TGTGGCTGCC 
TGCCGAGCAA ACGTGAAAAG CGGGGCCATC ATGTCGGCCC TGTCCGACAC AGAGATCCAG 
CGTGAGATTG GCATCAGCAA CCCCCTGCAC AGGCTGAAGC TGAGGCTGGC CATCCAGGAG 
ATCATGTCGC TGACCAGCCC GTCTGCCCCG CCCACATCTA GAACGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( NM_003626.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003617.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 2, mRNA.

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CloneID	OHu55875
Clone ID Related Accession (Same CDS sequence)	NM_003626.3 , NM_003626.4
Accession Version	NM_003626.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW163189.1, U22816.1, AK124621.1 and CK902590.1. On Oct 14, 2011 this sequence version replaced NM_003626.2. Transcript Variant: This variant (2) represents the longest transcript and it encodes the longer protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034046.1, SRR1803611.11048.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTTTGCCAC CTTCCAGAGA AGAGGTACGA GATGACAAGA CAACCATAAA GTGTGAAACC 
TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG TTAGACCGGC TGCACAAAGG GGCGCTGCAC 
ACCGTCAGCC ACGAGGACAT CAGGGACATA AGGAACTCCA CAGGCTCCCA GGATGGTCCC 
GTGAGCAACC CCAGCAGTAG CAACAGTAGC CAGGACTCGC TCCACAAAGC CCCAAAGAAG 
AAAGGCATTA AGTCCTCCAT TGGCCGCTTG TTTGGCAAGA AAGAAAAGGG CCGACCTGGA 
CAAACTGGCA AAGAAGCATT AGGACAAGCT GGTGTTTCCG AGACGGATAA CTCATCTCAG 
GATGCCTTGG GACTTAGCAA ATTGGGGGGA CAGGCTGAAA AAAATCGTAA ACTTCAAAAA 
AAGCATGAAT TGCTGGAGGA AGCCCGGAGA CAAGGTTTAC CTTTTGCCCA ATGGGACGGG 
CCAACGGTTG TGGTCTGGCT AGAGCTCTGG GTTGGGATGC CAGCCTGGTA TGTGGCTGCC 
TGCCGAGCAA ACGTGAAAAG CGGGGCCATC ATGTCGGCCC TGTCCGACAC AGAGATCCAG 
CGTGAGATTG GCATCAGCAA CCCCCTGCAC AGGCTGAAGC TGAGGCTGGC CATCCAGGAG 
ATCATGTCGC TGACCAGCCC GTCTGCCCCG CCCACATCTA GAACGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GCAATGTATC AGGAACACAG AGGTTGGATT CTGCTACAGT CAGGACTTAC 
TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003617.1
CDS	216..3824
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	NM_003626.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003626.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTTTGCCAC CTTCCAGAGA AGAGGTACGA GATGACAAGA CAACCATAAA GTGTGAAACC 
TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG TTAGACCGGC TGCACAAAGG GGCGCTGCAC 
ACCGTCAGCC ACGAGGACAT CAGGGACATA AGGAACTCCA CAGGCTCCCA GGATGGTCCC 
GTGAGCAACC CCAGCAGTAG CAACAGTAGC CAGGACTCGC TCCACAAAGC CCCAAAGAAG 
AAAGGCATTA AGTCCTCCAT TGGCCGCTTG TTTGGCAAGA AAGAAAAGGG CCGACCTGGA 
CAAACTGGCA AAGAAGCATT AGGACAAGCT GGTGTTTCCG AGACGGATAA CTCATCTCAG 
GATGCCTTGG GACTTAGCAA ATTGGGGGGA CAGGCTGAAA AAAATCGTAA ACTTCAAAAA 
AAGCATGAAT TGCTGGAGGA AGCCCGGAGA CAAGGTTTAC CTTTTGCCCA ATGGGACGGG 
CCAACGGTTG TGGTCTGGCT AGAGCTCTGG GTTGGGATGC CAGCCTGGTA TGTGGCTGCC 
TGCCGAGCAA ACGTGAAAAG CGGGGCCATC ATGTCGGCCC TGTCCGACAC AGAGATCCAG 
CGTGAGATTG GCATCAGCAA CCCCCTGCAC AGGCTGAAGC TGAGGCTGGC CATCCAGGAG 
ATCATGTCGC TGACCAGCCC GTCTGCCCCG CCCACATCTA GAACGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GCAATGTATC AGGAACACAG AGGTTGGATT CTGCTACAGT CAGGACTTAC 
TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( NM_003626.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003617.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 2, mRNA.

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CloneID	OHu55875
Clone ID Related Accession (Same CDS sequence)	NM_003626.3 , NM_003626.4
Accession Version	NM_003626.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP002336.5, AW163189.1, U22816.1, AK124621.1, CK902590.1 and AP000487.6. On Jun 2, 2019 this sequence version replaced NM_003626.3. Transcript Variant: This variant (2) represents the longest transcript and it encodes the longer protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.11048.1, BC034046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTTTGCCAC CTTCCAGAGA AGAGGTACGA GATGACAAGA CAACCATAAA GTGTGAAACC 
TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG TTAGACCGGC TGCACAAAGG GGCGCTGCAC 
ACCGTCAGCC ACGAGGACAT CAGGGACATA AGGAACTCCA CAGGCTCCCA GGATGGTCCC 
GTGAGCAACC CCAGCAGTAG CAACAGTAGC CAGGACTCGC TCCACAAAGC CCCAAAGAAG 
AAAGGCATTA AGTCCTCCAT TGGCCGCTTG TTTGGCAAGA AAGAAAAGGG CCGACCTGGA 
CAAACTGGCA AAGAAGCATT AGGACAAGCT GGTGTTTCCG AGACGGATAA CTCATCTCAG 
GATGCCTTGG GACTTAGCAA ATTGGGGGGA CAGGCTGAAA AAAATCGTAA ACTTCAAAAA 
AAGCATGAAT TGCTGGAGGA AGCCCGGAGA CAAGGTTTAC CTTTTGCCCA ATGGGACGGG 
CCAACGGTTG TGGTCTGGCT AGAGCTCTGG GTTGGGATGC CAGCCTGGTA TGTGGCTGCC 
TGCCGAGCAA ACGTGAAAAG CGGGGCCATC ATGTCGGCCC TGTCCGACAC AGAGATCCAG 
CGTGAGATTG GCATCAGCAA CCCCCTGCAC AGGCTGAAGC TGAGGCTGGC CATCCAGGAG 
ATCATGTCGC TGACCAGCCC GTCTGCCCCG CCCACATCTA GAACGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GCAATGTATC AGGAACACAG AGGTTGGATT CTGCTACAGT CAGGACTTAC 
TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003617.1
CDS	226..3834
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	NM_003626.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003626.4

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTTTGCCAC CTTCCAGAGA AGAGGTACGA GATGACAAGA CAACCATAAA GTGTGAAACC 
TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG TTAGACCGGC TGCACAAAGG GGCGCTGCAC 
ACCGTCAGCC ACGAGGACAT CAGGGACATA AGGAACTCCA CAGGCTCCCA GGATGGTCCC 
GTGAGCAACC CCAGCAGTAG CAACAGTAGC CAGGACTCGC TCCACAAAGC CCCAAAGAAG 
AAAGGCATTA AGTCCTCCAT TGGCCGCTTG TTTGGCAAGA AAGAAAAGGG CCGACCTGGA 
CAAACTGGCA AAGAAGCATT AGGACAAGCT GGTGTTTCCG AGACGGATAA CTCATCTCAG 
GATGCCTTGG GACTTAGCAA ATTGGGGGGA CAGGCTGAAA AAAATCGTAA ACTTCAAAAA 
AAGCATGAAT TGCTGGAGGA AGCCCGGAGA CAAGGTTTAC CTTTTGCCCA ATGGGACGGG 
CCAACGGTTG TGGTCTGGCT AGAGCTCTGG GTTGGGATGC CAGCCTGGTA TGTGGCTGCC 
TGCCGAGCAA ACGTGAAAAG CGGGGCCATC ATGTCGGCCC TGTCCGACAC AGAGATCCAG 
CGTGAGATTG GCATCAGCAA CCCCCTGCAC AGGCTGAAGC TGAGGCTGGC CATCCAGGAG 
ATCATGTCGC TGACCAGCCC GTCTGCCCCG CCCACATCTA GAACGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GCAATGTATC AGGAACACAG AGGTTGGATT CTGCTACAGT CAGGACTTAC 
TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545306.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543608.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.

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CloneID	OHu45568
Clone ID Related Accession (Same CDS sequence)	XM_011545306.2 , XM_011545306.3 , XM_006718716.3
Accession Version	XM_011545306.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543608.1
CDS	101..4021
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545306.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545306.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545306.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543608.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.

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CloneID	OHu45568
Clone ID Related Accession (Same CDS sequence)	XM_011545306.2 , XM_011545306.3 , XM_006718716.3
Accession Version	XM_011545306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545306.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543608.1
CDS	101..4021
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545306.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545306.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_006718716.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718779.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X1, mRNA.

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CloneID	OHu45568
Clone ID Related Accession (Same CDS sequence)	XM_011545306.2 , XM_011545306.3 , XM_006718716.3
Accession Version	XM_006718716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718779.1
CDS	229..4149
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_006718716.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718716.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGGCAATGTA TCAGGAACAC AGAGGTTGGA TTCTGCTACA 
GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545307.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543609.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61861
Clone ID Related Accession (Same CDS sequence)	XM_011545307.3 , XM_011545307.2
Accession Version	XM_011545307.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545307.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543609.1
CDS	229..4122
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545307.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545307.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545307.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543609.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61861
Clone ID Related Accession (Same CDS sequence)	XM_011545307.3 , XM_011545307.2
Accession Version	XM_011545307.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543609.1
CDS	229..4122
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545307.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545307.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545308.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543610.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.

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CloneID	OHu61862
Clone ID Related Accession (Same CDS sequence)	XM_011545308.3 , XM_011545308.2
Accession Version	XM_011545308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGTTGCC ACCTTCCAGA GAAGAGGTAC GAGATGACAA GACAACCATA 
AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG AGAGCCCTTC GGTTAGACCG GCTGCACAAA 
GGGGCGCTGC ACACCGTCAG CCACGAGGAC ATCAGGGACA TAAGGAACTC CACAGGCTCC 
CAGGATGGTC CCGTGAGCAA CCCCAGCAGT AGCAACAGTA GCCAGGACTC GCTCCACAAA 
GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG 
GGCCGACCTG GACAAACTGG CAAAGAAGCA TTAGGACAAG CTGGTGTTTC CGAGACGGAT 
AACTCATCTC AGGATGCCTT GGGACTTAGC AAATTGGGGG GACAGGCTGA AAAAAATCGT 
AAACTTCAAA AAAAGCATGA ATTGCTGGAG GAAGCCCGGA GACAAGGTTT ACCTTTTGCC 
CAATGGGACG GGCCAACGGT TGTGGTCTGG CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG 
TATGTGGCTG CCTGCCGAGC AAACGTGAAA AGCGGGGCCA TCATGTCGGC CCTGTCCGAC 
ACAGAGATCC AGCGTGAGAT TGGCATCAGC AACCCCCTGC ACAGGCTGAA GCTGAGGCTG 
GCCATCCAGG AGATCATGTC GCTGACCAGC CCGTCTGCCC CGCCCACATC TAGAACGACC 
ACAGGAAATG TCTGGTTAAC ACACGAAGAG ATGGAAACGC TCGCAGCCAC GCCGCAAACG 
GAAGATGAGG AGGGAAGCTG GGCTCAGACA CTCGCCTATG GGGACATGAA CCACGAGTGG 
ATCGGCAACG AGTGGCTCCC CAGCCTGGGC CTCCCCCAGT ACCGCAGCTA CTTCATGGAG 
TGCCTTGTAG ACGCCAGGAT GCTGGACCAC TTGACCAAGA AAGACCTTCG AGGGCAGCTG 
AAAATGGTCG ACAGTTTTCA CAGAAACAGT TTCCAGTGTG GAATTATGTG CCTGAGAAGG 
TTAAATTATG ACCGGAAAGA ACTGGAAAGA AAAAGAGAAG AAAGTCAGAG TGAAATAAAA 
GACGTGCTTG TTTGGAGCAA TGATCGAGTG ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA 
GAATATGCAA ACAATCTTAT AGAGAGTGGT GTTCACGGAG CACTTCTGGC CTTAGATGAA 
ACCTTCGACT TCAGTGCACT GGCACTGCTG TTACAGATCC CGACGCAGAA CACACAGGCT 
CGTGCTGTCT TGGAAAGAGA ATTTAACAAC CTTTTGGTCA TGGGGACTGA TAGAAGGTTT 
GATGAAGATG ATGATAAAAG CTTTAGGAGA GCACCTTCAT GGAGAAAAAA GTTTAGACCA 
AAGGACATTC GTGGCTTAGC TGCTGGGTCA GCAGAGACTC TCCCTGCAAA CTTCCGGGTG 
ACTTCTTCTA TGTCTTCCCC CTCTATGCAG CCAAAGAAGA TGCAGATGGA CGGCAATGTA 
TCAGGAACAC AGAGGTTGGA TTCTGCTACA GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543610.1
CDS	229..4119
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545308.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545308.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGTTGCC ACCTTCCAGA GAAGAGGTAC GAGATGACAA GACAACCATA 
AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG AGAGCCCTTC GGTTAGACCG GCTGCACAAA 
GGGGCGCTGC ACACCGTCAG CCACGAGGAC ATCAGGGACA TAAGGAACTC CACAGGCTCC 
CAGGATGGTC CCGTGAGCAA CCCCAGCAGT AGCAACAGTA GCCAGGACTC GCTCCACAAA 
GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG 
GGCCGACCTG GACAAACTGG CAAAGAAGCA TTAGGACAAG CTGGTGTTTC CGAGACGGAT 
AACTCATCTC AGGATGCCTT GGGACTTAGC AAATTGGGGG GACAGGCTGA AAAAAATCGT 
AAACTTCAAA AAAAGCATGA ATTGCTGGAG GAAGCCCGGA GACAAGGTTT ACCTTTTGCC 
CAATGGGACG GGCCAACGGT TGTGGTCTGG CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG 
TATGTGGCTG CCTGCCGAGC AAACGTGAAA AGCGGGGCCA TCATGTCGGC CCTGTCCGAC 
ACAGAGATCC AGCGTGAGAT TGGCATCAGC AACCCCCTGC ACAGGCTGAA GCTGAGGCTG 
GCCATCCAGG AGATCATGTC GCTGACCAGC CCGTCTGCCC CGCCCACATC TAGAACGACC 
ACAGGAAATG TCTGGTTAAC ACACGAAGAG ATGGAAACGC TCGCAGCCAC GCCGCAAACG 
GAAGATGAGG AGGGAAGCTG GGCTCAGACA CTCGCCTATG GGGACATGAA CCACGAGTGG 
ATCGGCAACG AGTGGCTCCC CAGCCTGGGC CTCCCCCAGT ACCGCAGCTA CTTCATGGAG 
TGCCTTGTAG ACGCCAGGAT GCTGGACCAC TTGACCAAGA AAGACCTTCG AGGGCAGCTG 
AAAATGGTCG ACAGTTTTCA CAGAAACAGT TTCCAGTGTG GAATTATGTG CCTGAGAAGG 
TTAAATTATG ACCGGAAAGA ACTGGAAAGA AAAAGAGAAG AAAGTCAGAG TGAAATAAAA 
GACGTGCTTG TTTGGAGCAA TGATCGAGTG ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA 
GAATATGCAA ACAATCTTAT AGAGAGTGGT GTTCACGGAG CACTTCTGGC CTTAGATGAA 
ACCTTCGACT TCAGTGCACT GGCACTGCTG TTACAGATCC CGACGCAGAA CACACAGGCT 
CGTGCTGTCT TGGAAAGAGA ATTTAACAAC CTTTTGGTCA TGGGGACTGA TAGAAGGTTT 
GATGAAGATG ATGATAAAAG CTTTAGGAGA GCACCTTCAT GGAGAAAAAA GTTTAGACCA 
AAGGACATTC GTGGCTTAGC TGCTGGGTCA GCAGAGACTC TCCCTGCAAA CTTCCGGGTG 
ACTTCTTCTA TGTCTTCCCC CTCTATGCAG CCAAAGAAGA TGCAGATGGA CGGCAATGTA 
TCAGGAACAC AGAGGTTGGA TTCTGCTACA GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545308.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543610.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu61862
Clone ID Related Accession (Same CDS sequence)	XM_011545308.3 , XM_011545308.2
Accession Version	XM_011545308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGTTGCC ACCTTCCAGA GAAGAGGTAC GAGATGACAA GACAACCATA 
AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG AGAGCCCTTC GGTTAGACCG GCTGCACAAA 
GGGGCGCTGC ACACCGTCAG CCACGAGGAC ATCAGGGACA TAAGGAACTC CACAGGCTCC 
CAGGATGGTC CCGTGAGCAA CCCCAGCAGT AGCAACAGTA GCCAGGACTC GCTCCACAAA 
GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG 
GGCCGACCTG GACAAACTGG CAAAGAAGCA TTAGGACAAG CTGGTGTTTC CGAGACGGAT 
AACTCATCTC AGGATGCCTT GGGACTTAGC AAATTGGGGG GACAGGCTGA AAAAAATCGT 
AAACTTCAAA AAAAGCATGA ATTGCTGGAG GAAGCCCGGA GACAAGGTTT ACCTTTTGCC 
CAATGGGACG GGCCAACGGT TGTGGTCTGG CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG 
TATGTGGCTG CCTGCCGAGC AAACGTGAAA AGCGGGGCCA TCATGTCGGC CCTGTCCGAC 
ACAGAGATCC AGCGTGAGAT TGGCATCAGC AACCCCCTGC ACAGGCTGAA GCTGAGGCTG 
GCCATCCAGG AGATCATGTC GCTGACCAGC CCGTCTGCCC CGCCCACATC TAGAACGACC 
ACAGGAAATG TCTGGTTAAC ACACGAAGAG ATGGAAACGC TCGCAGCCAC GCCGCAAACG 
GAAGATGAGG AGGGAAGCTG GGCTCAGACA CTCGCCTATG GGGACATGAA CCACGAGTGG 
ATCGGCAACG AGTGGCTCCC CAGCCTGGGC CTCCCCCAGT ACCGCAGCTA CTTCATGGAG 
TGCCTTGTAG ACGCCAGGAT GCTGGACCAC TTGACCAAGA AAGACCTTCG AGGGCAGCTG 
AAAATGGTCG ACAGTTTTCA CAGAAACAGT TTCCAGTGTG GAATTATGTG CCTGAGAAGG 
TTAAATTATG ACCGGAAAGA ACTGGAAAGA AAAAGAGAAG AAAGTCAGAG TGAAATAAAA 
GACGTGCTTG TTTGGAGCAA TGATCGAGTG ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA 
GAATATGCAA ACAATCTTAT AGAGAGTGGT GTTCACGGAG CACTTCTGGC CTTAGATGAA 
ACCTTCGACT TCAGTGCACT GGCACTGCTG TTACAGATCC CGACGCAGAA CACACAGGCT 
CGTGCTGTCT TGGAAAGAGA ATTTAACAAC CTTTTGGTCA TGGGGACTGA TAGAAGGTTT 
GATGAAGATG ATGATAAAAG CTTTAGGAGA GCACCTTCAT GGAGAAAAAA GTTTAGACCA 
AAGGACATTC GTGGCTTAGC TGCTGGGTCA GCAGAGACTC TCCCTGCAAA CTTCCGGGTG 
ACTTCTTCTA TGTCTTCCCC CTCTATGCAG CCAAAGAAGA TGCAGATGGA CGGCAATGTA 
TCAGGAACAC AGAGGTTGGA TTCTGCTACA GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543610.1
CDS	229..4119
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545308.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545308.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGTTGCC ACCTTCCAGA GAAGAGGTAC GAGATGACAA GACAACCATA 
AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG AGAGCCCTTC GGTTAGACCG GCTGCACAAA 
GGGGCGCTGC ACACCGTCAG CCACGAGGAC ATCAGGGACA TAAGGAACTC CACAGGCTCC 
CAGGATGGTC CCGTGAGCAA CCCCAGCAGT AGCAACAGTA GCCAGGACTC GCTCCACAAA 
GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG 
GGCCGACCTG GACAAACTGG CAAAGAAGCA TTAGGACAAG CTGGTGTTTC CGAGACGGAT 
AACTCATCTC AGGATGCCTT GGGACTTAGC AAATTGGGGG GACAGGCTGA AAAAAATCGT 
AAACTTCAAA AAAAGCATGA ATTGCTGGAG GAAGCCCGGA GACAAGGTTT ACCTTTTGCC 
CAATGGGACG GGCCAACGGT TGTGGTCTGG CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG 
TATGTGGCTG CCTGCCGAGC AAACGTGAAA AGCGGGGCCA TCATGTCGGC CCTGTCCGAC 
ACAGAGATCC AGCGTGAGAT TGGCATCAGC AACCCCCTGC ACAGGCTGAA GCTGAGGCTG 
GCCATCCAGG AGATCATGTC GCTGACCAGC CCGTCTGCCC CGCCCACATC TAGAACGACC 
ACAGGAAATG TCTGGTTAAC ACACGAAGAG ATGGAAACGC TCGCAGCCAC GCCGCAAACG 
GAAGATGAGG AGGGAAGCTG GGCTCAGACA CTCGCCTATG GGGACATGAA CCACGAGTGG 
ATCGGCAACG AGTGGCTCCC CAGCCTGGGC CTCCCCCAGT ACCGCAGCTA CTTCATGGAG 
TGCCTTGTAG ACGCCAGGAT GCTGGACCAC TTGACCAAGA AAGACCTTCG AGGGCAGCTG 
AAAATGGTCG ACAGTTTTCA CAGAAACAGT TTCCAGTGTG GAATTATGTG CCTGAGAAGG 
TTAAATTATG ACCGGAAAGA ACTGGAAAGA AAAAGAGAAG AAAGTCAGAG TGAAATAAAA 
GACGTGCTTG TTTGGAGCAA TGATCGAGTG ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA 
GAATATGCAA ACAATCTTAT AGAGAGTGGT GTTCACGGAG CACTTCTGGC CTTAGATGAA 
ACCTTCGACT TCAGTGCACT GGCACTGCTG TTACAGATCC CGACGCAGAA CACACAGGCT 
CGTGCTGTCT TGGAAAGAGA ATTTAACAAC CTTTTGGTCA TGGGGACTGA TAGAAGGTTT 
GATGAAGATG ATGATAAAAG CTTTAGGAGA GCACCTTCAT GGAGAAAAAA GTTTAGACCA 
AAGGACATTC GTGGCTTAGC TGCTGGGTCA GCAGAGACTC TCCCTGCAAA CTTCCGGGTG 
ACTTCTTCTA TGTCTTCCCC CTCTATGCAG CCAAAGAAGA TGCAGATGGA CGGCAATGTA 
TCAGGAACAC AGAGGTTGGA TTCTGCTACA GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545309.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543611.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu61863
Clone ID Related Accession (Same CDS sequence)	XM_011545309.2
Accession Version	XM_011545309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGTTTACCCA CACTACTTCT ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543611.1
CDS	229..4116
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDVYPHYFYR

Target ORF information:

RefSeq Version	XM_011545309.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545309.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACC ACAGGAAATG TCTGGTTAAC ACACGAAGAG 
ATGGAAACGC TCGCAGCCAC GCCGCAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
CTCGCCTATG GGGACATGAA CCACGAGTGG ATCGGCAACG AGTGGCTCCC CAGCCTGGGC 
CTCCCCCAGT ACCGCAGCTA CTTCATGGAG TGCCTTGTAG ACGCCAGGAT GCTGGACCAC 
TTGACCAAGA AAGACCTTCG AGGGCAGCTG AAAATGGTCG ACAGTTTTCA CAGAAACAGT 
TTCCAGTGTG GAATTATGTG CCTGAGAAGG TTAAATTATG ACCGGAAAGA ACTGGAAAGA 
AAAAGAGAAG AAAGTCAGAG TGAAATAAAA GACGTGCTTG TTTGGAGCAA TGATCGAGTG 
ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA GAATATGCAA ACAATCTTAT AGAGAGTGGT 
GTTCACGGAG CACTTCTGGC CTTAGATGAA ACCTTCGACT TCAGTGCACT GGCACTGCTG 
TTACAGATCC CGACGCAGAA CACACAGGCT CGTGCTGTCT TGGAAAGAGA ATTTAACAAC 
CTTTTGGTCA TGGGGACTGA TAGAAGGTTT GATGAAGATG ATGATAAAAG CTTTAGGAGA 
GCACCTTCAT GGAGAAAAAA GTTTAGACCA AAGGACATTC GTGGCTTAGC TGCTGGGTCA 
GCAGAGACTC TCCCTGCAAA CTTCCGGGTG ACTTCTTCTA TGTCTTCCCC CTCTATGCAG 
CCAAAGAAGA TGCAGATGGA CGTTTACCCA CACTACTTCT ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545310.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543612.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.

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CloneID	OHu61864
Clone ID Related Accession (Same CDS sequence)	XM_011545310.2 , XM_011545310.3
Accession Version	XM_011545310.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCATCTC CAGATTCATT TTTAATCCAG ACCTCAGGTC CCCATCAGTC AGTAGTTTAC 
TCATCCACCT CCCCTCCCAG TTCCACCCCA TGCTATAGCG ATTCTTCACA GCATGCTCAG 
CCTAGTGATT TAAGGAAACA TCGTAGAAAG TTGCCACCTT CCAGAGAAGA GGTACGAGAT 
GACAAGACAA CCATAAAGTG TGAAACCTCC CCGCCTTCCT CCCCGAGAGC CCTTCGGTTA 
GACCGGCTGC ACAAAGGGGC GCTGCACACC GTCAGCCACG AGGACATCAG GGACATAAGG 
AACTCCACAG GCTCCCAGGA TGGTCCCGTG AGCAACCCCA GCAGTAGCAA CAGTAGCCAG 
GACTCGCTCC ACAAAGCCCC AAAGAAGAAA GGCATTAAGT CCTCCATTGG CCGCTTGTTT 
GGCAAGAAAG AAAAGGGCCG ACCTGGACAA ACTGGCAAAG AAGCATTAGG ACAAGCTGGT 
GTTTCCGAGA CGGATAACTC ATCTCAGGAT GCCTTGGGAC TTAGCAAATT GGGGGGACAG 
GCTGAAAAAA ATCGTAAACT TCAAAAAAAG CATGAATTGC TGGAGGAAGC CCGGAGACAA 
GGTTTACCTT TTGCCCAATG GGACGGGCCA ACGGTTGTGG TCTGGCTAGA GCTCTGGGTT 
GGGATGCCAG CCTGGTATGT GGCTGCCTGC CGAGCAAACG TGAAAAGCGG GGCCATCATG 
TCGGCCCTGT CCGACACAGA GATCCAGCGT GAGATTGGCA TCAGCAACCC CCTGCACAGG 
CTGAAGCTGA GGCTGGCCAT CCAGGAGATC ATGTCGCTGA CCAGCCCGTC TGCCCCGCCC 
ACATCTAGAA CGACCACAGG AAATGTCTGG TTAACACACG AAGAGATGGA AACGCTCGCA 
GCCACGCCGC AAACGGAAGA TGAGGAGGGA AGCTGGGCTC AGACACTCGC CTATGGGGAC 
ATGAACCACG AGTGGATCGG CAACGAGTGG CTCCCCAGCC TGGGCCTCCC CCAGTACCGC 
AGCTACTTCA TGGAGTGCCT TGTAGACGCC AGGATGCTGG ACCACTTGAC CAAGAAAGAC 
CTTCGAGGGC AGCTGAAAAT GGTCGACAGT TTTCACAGAA ACAGTTTCCA GTGTGGAATT 
ATGTGCCTGA GAAGGTTAAA TTATGACCGG AAAGAACTGG AAAGAAAAAG AGAAGAAAGT 
CAGAGTGAAA TAAAAGACGT GCTTGTTTGG AGCAATGATC GAGTGATTCG CTGGATCCTG 
TCAATTGGCC TTAAAGAATA TGCAAACAAT CTTATAGAGA GTGGTGTTCA CGGAGCACTT 
CTGGCCTTAG ATGAAACCTT CGACTTCAGT GCACTGGCAC TGCTGTTACA GATCCCGACG 
CAGAACACAC AGGCTCGTGC TGTCTTGGAA AGAGAATTTA ACAACCTTTT GGTCATGGGG 
ACTGATAGAA GGTTTGATGA AGATGATGAT AAAAGCTTTA GGAGAGCACC TTCATGGAGA 
AAAAAGTTTA GACCAAAGGA CATTCGTGGC TTAGCTGCTG GGTCAGCAGA GACTCTCCCT 
GCAAACTTCC GGGTGACTTC TTCTATGTCT TCCCCCTCTA TGCAGCCAAA GAAGATGCAG 
ATGGACGGCA ATGTATCAGG AACACAGAGG TTGGATTCTG CTACAGTCAG GACTTACTCC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543612.1
CDS	229..4074
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545310.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545310.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCATCTC CAGATTCATT TTTAATCCAG ACCTCAGGTC CCCATCAGTC AGTAGTTTAC 
TCATCCACCT CCCCTCCCAG TTCCACCCCA TGCTATAGCG ATTCTTCACA GCATGCTCAG 
CCTAGTGATT TAAGGAAACA TCGTAGAAAG TTGCCACCTT CCAGAGAAGA GGTACGAGAT 
GACAAGACAA CCATAAAGTG TGAAACCTCC CCGCCTTCCT CCCCGAGAGC CCTTCGGTTA 
GACCGGCTGC ACAAAGGGGC GCTGCACACC GTCAGCCACG AGGACATCAG GGACATAAGG 
AACTCCACAG GCTCCCAGGA TGGTCCCGTG AGCAACCCCA GCAGTAGCAA CAGTAGCCAG 
GACTCGCTCC ACAAAGCCCC AAAGAAGAAA GGCATTAAGT CCTCCATTGG CCGCTTGTTT 
GGCAAGAAAG AAAAGGGCCG ACCTGGACAA ACTGGCAAAG AAGCATTAGG ACAAGCTGGT 
GTTTCCGAGA CGGATAACTC ATCTCAGGAT GCCTTGGGAC TTAGCAAATT GGGGGGACAG 
GCTGAAAAAA ATCGTAAACT TCAAAAAAAG CATGAATTGC TGGAGGAAGC CCGGAGACAA 
GGTTTACCTT TTGCCCAATG GGACGGGCCA ACGGTTGTGG TCTGGCTAGA GCTCTGGGTT 
GGGATGCCAG CCTGGTATGT GGCTGCCTGC CGAGCAAACG TGAAAAGCGG GGCCATCATG 
TCGGCCCTGT CCGACACAGA GATCCAGCGT GAGATTGGCA TCAGCAACCC CCTGCACAGG 
CTGAAGCTGA GGCTGGCCAT CCAGGAGATC ATGTCGCTGA CCAGCCCGTC TGCCCCGCCC 
ACATCTAGAA CGACCACAGG AAATGTCTGG TTAACACACG AAGAGATGGA AACGCTCGCA 
GCCACGCCGC AAACGGAAGA TGAGGAGGGA AGCTGGGCTC AGACACTCGC CTATGGGGAC 
ATGAACCACG AGTGGATCGG CAACGAGTGG CTCCCCAGCC TGGGCCTCCC CCAGTACCGC 
AGCTACTTCA TGGAGTGCCT TGTAGACGCC AGGATGCTGG ACCACTTGAC CAAGAAAGAC 
CTTCGAGGGC AGCTGAAAAT GGTCGACAGT TTTCACAGAA ACAGTTTCCA GTGTGGAATT 
ATGTGCCTGA GAAGGTTAAA TTATGACCGG AAAGAACTGG AAAGAAAAAG AGAAGAAAGT 
CAGAGTGAAA TAAAAGACGT GCTTGTTTGG AGCAATGATC GAGTGATTCG CTGGATCCTG 
TCAATTGGCC TTAAAGAATA TGCAAACAAT CTTATAGAGA GTGGTGTTCA CGGAGCACTT 
CTGGCCTTAG ATGAAACCTT CGACTTCAGT GCACTGGCAC TGCTGTTACA GATCCCGACG 
CAGAACACAC AGGCTCGTGC TGTCTTGGAA AGAGAATTTA ACAACCTTTT GGTCATGGGG 
ACTGATAGAA GGTTTGATGA AGATGATGAT AAAAGCTTTA GGAGAGCACC TTCATGGAGA 
AAAAAGTTTA GACCAAAGGA CATTCGTGGC TTAGCTGCTG GGTCAGCAGA GACTCTCCCT 
GCAAACTTCC GGGTGACTTC TTCTATGTCT TCCCCCTCTA TGCAGCCAAA GAAGATGCAG 
ATGGACGGCA ATGTATCAGG AACACAGAGG TTGGATTCTG CTACAGTCAG GACTTACTCC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545310.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543612.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu61864
Clone ID Related Accession (Same CDS sequence)	XM_011545310.2 , XM_011545310.3
Accession Version	XM_011545310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCATCTC CAGATTCATT TTTAATCCAG ACCTCAGGTC CCCATCAGTC AGTAGTTTAC 
TCATCCACCT CCCCTCCCAG TTCCACCCCA TGCTATAGCG ATTCTTCACA GCATGCTCAG 
CCTAGTGATT TAAGGAAACA TCGTAGAAAG TTGCCACCTT CCAGAGAAGA GGTACGAGAT 
GACAAGACAA CCATAAAGTG TGAAACCTCC CCGCCTTCCT CCCCGAGAGC CCTTCGGTTA 
GACCGGCTGC ACAAAGGGGC GCTGCACACC GTCAGCCACG AGGACATCAG GGACATAAGG 
AACTCCACAG GCTCCCAGGA TGGTCCCGTG AGCAACCCCA GCAGTAGCAA CAGTAGCCAG 
GACTCGCTCC ACAAAGCCCC AAAGAAGAAA GGCATTAAGT CCTCCATTGG CCGCTTGTTT 
GGCAAGAAAG AAAAGGGCCG ACCTGGACAA ACTGGCAAAG AAGCATTAGG ACAAGCTGGT 
GTTTCCGAGA CGGATAACTC ATCTCAGGAT GCCTTGGGAC TTAGCAAATT GGGGGGACAG 
GCTGAAAAAA ATCGTAAACT TCAAAAAAAG CATGAATTGC TGGAGGAAGC CCGGAGACAA 
GGTTTACCTT TTGCCCAATG GGACGGGCCA ACGGTTGTGG TCTGGCTAGA GCTCTGGGTT 
GGGATGCCAG CCTGGTATGT GGCTGCCTGC CGAGCAAACG TGAAAAGCGG GGCCATCATG 
TCGGCCCTGT CCGACACAGA GATCCAGCGT GAGATTGGCA TCAGCAACCC CCTGCACAGG 
CTGAAGCTGA GGCTGGCCAT CCAGGAGATC ATGTCGCTGA CCAGCCCGTC TGCCCCGCCC 
ACATCTAGAA CGACCACAGG AAATGTCTGG TTAACACACG AAGAGATGGA AACGCTCGCA 
GCCACGCCGC AAACGGAAGA TGAGGAGGGA AGCTGGGCTC AGACACTCGC CTATGGGGAC 
ATGAACCACG AGTGGATCGG CAACGAGTGG CTCCCCAGCC TGGGCCTCCC CCAGTACCGC 
AGCTACTTCA TGGAGTGCCT TGTAGACGCC AGGATGCTGG ACCACTTGAC CAAGAAAGAC 
CTTCGAGGGC AGCTGAAAAT GGTCGACAGT TTTCACAGAA ACAGTTTCCA GTGTGGAATT 
ATGTGCCTGA GAAGGTTAAA TTATGACCGG AAAGAACTGG AAAGAAAAAG AGAAGAAAGT 
CAGAGTGAAA TAAAAGACGT GCTTGTTTGG AGCAATGATC GAGTGATTCG CTGGATCCTG 
TCAATTGGCC TTAAAGAATA TGCAAACAAT CTTATAGAGA GTGGTGTTCA CGGAGCACTT 
CTGGCCTTAG ATGAAACCTT CGACTTCAGT GCACTGGCAC TGCTGTTACA GATCCCGACG 
CAGAACACAC AGGCTCGTGC TGTCTTGGAA AGAGAATTTA ACAACCTTTT GGTCATGGGG 
ACTGATAGAA GGTTTGATGA AGATGATGAT AAAAGCTTTA GGAGAGCACC TTCATGGAGA 
AAAAAGTTTA GACCAAAGGA CATTCGTGGC TTAGCTGCTG GGTCAGCAGA GACTCTCCCT 
GCAAACTTCC GGGTGACTTC TTCTATGTCT TCCCCCTCTA TGCAGCCAAA GAAGATGCAG 
ATGGACGGCA ATGTATCAGG AACACAGAGG TTGGATTCTG CTACAGTCAG GACTTACTCC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543612.1
CDS	229..4074
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545310.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545310.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCATCTC CAGATTCATT TTTAATCCAG ACCTCAGGTC CCCATCAGTC AGTAGTTTAC 
TCATCCACCT CCCCTCCCAG TTCCACCCCA TGCTATAGCG ATTCTTCACA GCATGCTCAG 
CCTAGTGATT TAAGGAAACA TCGTAGAAAG TTGCCACCTT CCAGAGAAGA GGTACGAGAT 
GACAAGACAA CCATAAAGTG TGAAACCTCC CCGCCTTCCT CCCCGAGAGC CCTTCGGTTA 
GACCGGCTGC ACAAAGGGGC GCTGCACACC GTCAGCCACG AGGACATCAG GGACATAAGG 
AACTCCACAG GCTCCCAGGA TGGTCCCGTG AGCAACCCCA GCAGTAGCAA CAGTAGCCAG 
GACTCGCTCC ACAAAGCCCC AAAGAAGAAA GGCATTAAGT CCTCCATTGG CCGCTTGTTT 
GGCAAGAAAG AAAAGGGCCG ACCTGGACAA ACTGGCAAAG AAGCATTAGG ACAAGCTGGT 
GTTTCCGAGA CGGATAACTC ATCTCAGGAT GCCTTGGGAC TTAGCAAATT GGGGGGACAG 
GCTGAAAAAA ATCGTAAACT TCAAAAAAAG CATGAATTGC TGGAGGAAGC CCGGAGACAA 
GGTTTACCTT TTGCCCAATG GGACGGGCCA ACGGTTGTGG TCTGGCTAGA GCTCTGGGTT 
GGGATGCCAG CCTGGTATGT GGCTGCCTGC CGAGCAAACG TGAAAAGCGG GGCCATCATG 
TCGGCCCTGT CCGACACAGA GATCCAGCGT GAGATTGGCA TCAGCAACCC CCTGCACAGG 
CTGAAGCTGA GGCTGGCCAT CCAGGAGATC ATGTCGCTGA CCAGCCCGTC TGCCCCGCCC 
ACATCTAGAA CGACCACAGG AAATGTCTGG TTAACACACG AAGAGATGGA AACGCTCGCA 
GCCACGCCGC AAACGGAAGA TGAGGAGGGA AGCTGGGCTC AGACACTCGC CTATGGGGAC 
ATGAACCACG AGTGGATCGG CAACGAGTGG CTCCCCAGCC TGGGCCTCCC CCAGTACCGC 
AGCTACTTCA TGGAGTGCCT TGTAGACGCC AGGATGCTGG ACCACTTGAC CAAGAAAGAC 
CTTCGAGGGC AGCTGAAAAT GGTCGACAGT TTTCACAGAA ACAGTTTCCA GTGTGGAATT 
ATGTGCCTGA GAAGGTTAAA TTATGACCGG AAAGAACTGG AAAGAAAAAG AGAAGAAAGT 
CAGAGTGAAA TAAAAGACGT GCTTGTTTGG AGCAATGATC GAGTGATTCG CTGGATCCTG 
TCAATTGGCC TTAAAGAATA TGCAAACAAT CTTATAGAGA GTGGTGTTCA CGGAGCACTT 
CTGGCCTTAG ATGAAACCTT CGACTTCAGT GCACTGGCAC TGCTGTTACA GATCCCGACG 
CAGAACACAC AGGCTCGTGC TGTCTTGGAA AGAGAATTTA ACAACCTTTT GGTCATGGGG 
ACTGATAGAA GGTTTGATGA AGATGATGAT AAAAGCTTTA GGAGAGCACC TTCATGGAGA 
AAAAAGTTTA GACCAAAGGA CATTCGTGGC TTAGCTGCTG GGTCAGCAGA GACTCTCCCT 
GCAAACTTCC GGGTGACTTC TTCTATGTCT TCCCCCTCTA TGCAGCCAAA GAAGATGCAG 
ATGGACGGCA ATGTATCAGG AACACAGAGG TTGGATTCTG CTACAGTCAG GACTTACTCC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545311.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543613.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu61865
Clone ID Related Accession (Same CDS sequence)	XM_011545311.3 , XM_011545311.2
Accession Version	XM_011545311.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545311.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543613.1
CDS	229..4062
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545311.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545311.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545311.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543613.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.

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CloneID	OHu61865
Clone ID Related Accession (Same CDS sequence)	XM_011545311.3 , XM_011545311.2
Accession Version	XM_011545311.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543613.1
CDS	229..4062
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545311.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545311.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545312.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543614.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu61866
Clone ID Related Accession (Same CDS sequence)	XM_011545312.2
Accession Version	XM_011545312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACA CTCGCCTATG GGGACATGAA CCACGAGTGG 
ATCGGCAACG AGTGGCTCCC CAGCCTGGGC CTCCCCCAGT ACCGCAGCTA CTTCATGGAG 
TGCCTTGTAG ACGCCAGGAT GCTGGACCAC TTGACCAAGA AAGACCTTCG AGGGCAGCTG 
AAAATGGTCG ACAGTTTTCA CAGAAACAGT TTCCAGTGTG GAATTATGTG CCTGAGAAGG 
TTAAATTATG ACCGGAAAGA ACTGGAAAGA AAAAGAGAAG AAAGTCAGAG TGAAATAAAA 
GACGTGCTTG TTTGGAGCAA TGATCGAGTG ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA 
GAATATGCAA ACAATCTTAT AGAGAGTGGT GTTCACGGAG CACTTCTGGC CTTAGATGAA 
ACCTTCGACT TCAGTGCACT GGCACTGCTG TTACAGATCC CGACGCAGAA CACACAGGCT 
CGTGCTGTCT TGGAAAGAGA ATTTAACAAC CTTTTGGTCA TGGGGACTGA TAGAAGGTTT 
GATGAAGATG ATGATAAAAG CTTTAGGAGA GCACCTTCAT GGAGAAAAAA GTTTAGACCA 
AAGGACATTC GTGGCTTAGC TGCTGGGTCA GCAGAGACTC TCCCTGCAAA CTTCCGGGTG 
ACTTCTTCTA TGTCTTCCCC CTCTATGCAG CCAAAGAAGA TGCAGATGGA CGGCAATGTA 
TCAGGAACAC AGAGGTTGGA TTCTGCTACA GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543614.1
CDS	229..4059
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSPDSFLIQTSGPHQSVVYSSTSPPSSTPCYSDSSQHAQPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545312.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC ATCTCCAGAT TCATTTTTAA TCCAGACCTC AGGTCCCCAT 
CAGTCAGTAG TTTACTCATC CACCTCCCCT CCCAGTTCCA CCCCATGCTA TAGCGATTCT 
TCACAGCATG CTCAGCCTAG TGATTTAAGG AAACATCGTA GAAAGTTGCC ACCTTCCAGA 
GAAGAGGTAC GAGATGACAA GACAACCATA AAGTGTGAAA CCTCCCCGCC TTCCTCCCCG 
AGAGCCCTTC GGTTAGACCG GCTGCACAAA GGGGCGCTGC ACACCGTCAG CCACGAGGAC 
ATCAGGGACA TAAGGAACTC CACAGGCTCC CAGGATGGTC CCGTGAGCAA CCCCAGCAGT 
AGCAACAGTA GCCAGGACTC GCTCCACAAA GCCCCAAAGA AGAAAGGCAT TAAGTCCTCC 
ATTGGCCGCT TGTTTGGCAA GAAAGAAAAG GGCCGACCTG GACAAACTGG CAAAGAAGCA 
TTAGGACAAG CTGGTGTTTC CGAGACGGAT AACTCATCTC AGGATGCCTT GGGACTTAGC 
AAATTGGGGG GACAGGCTGA AAAAAATCGT AAACTTCAAA AAAAGCATGA ATTGCTGGAG 
GAAGCCCGGA GACAAGGTTT ACCTTTTGCC CAATGGGACG GGCCAACGGT TGTGGTCTGG 
CTAGAGCTCT GGGTTGGGAT GCCAGCCTGG TATGTGGCTG CCTGCCGAGC AAACGTGAAA 
AGCGGGGCCA TCATGTCGGC CCTGTCCGAC ACAGAGATCC AGCGTGAGAT TGGCATCAGC 
AACCCCCTGC ACAGGCTGAA GCTGAGGCTG GCCATCCAGG AGATCATGTC GCTGACCAGC 
CCGTCTGCCC CGCCCACATC TAGAACGACA CTCGCCTATG GGGACATGAA CCACGAGTGG 
ATCGGCAACG AGTGGCTCCC CAGCCTGGGC CTCCCCCAGT ACCGCAGCTA CTTCATGGAG 
TGCCTTGTAG ACGCCAGGAT GCTGGACCAC TTGACCAAGA AAGACCTTCG AGGGCAGCTG 
AAAATGGTCG ACAGTTTTCA CAGAAACAGT TTCCAGTGTG GAATTATGTG CCTGAGAAGG 
TTAAATTATG ACCGGAAAGA ACTGGAAAGA AAAAGAGAAG AAAGTCAGAG TGAAATAAAA 
GACGTGCTTG TTTGGAGCAA TGATCGAGTG ATTCGCTGGA TCCTGTCAAT TGGCCTTAAA 
GAATATGCAA ACAATCTTAT AGAGAGTGGT GTTCACGGAG CACTTCTGGC CTTAGATGAA 
ACCTTCGACT TCAGTGCACT GGCACTGCTG TTACAGATCC CGACGCAGAA CACACAGGCT 
CGTGCTGTCT TGGAAAGAGA ATTTAACAAC CTTTTGGTCA TGGGGACTGA TAGAAGGTTT 
GATGAAGATG ATGATAAAAG CTTTAGGAGA GCACCTTCAT GGAGAAAAAA GTTTAGACCA 
AAGGACATTC GTGGCTTAGC TGCTGGGTCA GCAGAGACTC TCCCTGCAAA CTTCCGGGTG 
ACTTCTTCTA TGTCTTCCCC CTCTATGCAG CCAAAGAAGA TGCAGATGGA CGGCAATGTA 
TCAGGAACAC AGAGGTTGGA TTCTGCTACA GTCAGGACTT ACTCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545313.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543615.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu61867
Clone ID Related Accession (Same CDS sequence)	XM_011545313.2
Accession Version	XM_011545313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC TAGTGATTTA AGGAAACATC GTAGAAAGTT GCCACCTTCC 
AGAGAAGAGG TACGAGATGA CAAGACAACC ATAAAGTGTG AAACCTCCCC GCCTTCCTCC 
CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC AAAGGGGCGC TGCACACCGT CAGCCACGAG 
GACATCAGGG ACATAAGGAA CTCCACAGGC TCCCAGGATG GTCCCGTGAG CAACCCCAGC 
AGTAGCAACA GTAGCCAGGA CTCGCTCCAC AAAGCCCCAA AGAAGAAAGG CATTAAGTCC 
TCCATTGGCC GCTTGTTTGG CAAGAAAGAA AAGGGCCGAC CTGGACAAAC TGGCAAAGAA 
GCATTAGGAC AAGCTGGTGT TTCCGAGACG GATAACTCAT CTCAGGATGC CTTGGGACTT 
AGCAAATTGG GGGGACAGGC TGAAAAAAAT CGTAAACTTC AAAAAAAGCA TGAATTGCTG 
GAGGAAGCCC GGAGACAAGG TTTACCTTTT GCCCAATGGG ACGGGCCAAC GGTTGTGGTC 
TGGCTAGAGC TCTGGGTTGG GATGCCAGCC TGGTATGTGG CTGCCTGCCG AGCAAACGTG 
AAAAGCGGGG CCATCATGTC GGCCCTGTCC GACACAGAGA TCCAGCGTGA GATTGGCATC 
AGCAACCCCC TGCACAGGCT GAAGCTGAGG CTGGCCATCC AGGAGATCAT GTCGCTGACC 
AGCCCGTCTG CCCCGCCCAC ATCTAGAACG ACCACAGGAA ATGTCTGGTT AACACACGAA 
GAGATGGAAA CGCTCGCAGC CACGCCGCAA ACGGAAGATG AGGAGGGAAG CTGGGCTCAG 
ACACTCGCCT ATGGGGACAT GAACCACGAG TGGATCGGCA ACGAGTGGCT CCCCAGCCTG 
GGCCTCCCCC AGTACCGCAG CTACTTCATG GAGTGCCTTG TAGACGCCAG GATGCTGGAC 
CACTTGACCA AGAAAGACCT TCGAGGGCAG CTGAAAATGG TCGACAGTTT TCACAGAAAC 
AGTTTCCAGT GTGGAATTAT GTGCCTGAGA AGGTTAAATT ATGACCGGAA AGAACTGGAA 
AGAAAAAGAG AAGAAAGTCA GAGTGAAATA AAAGACGTGC TTGTTTGGAG CAATGATCGA 
GTGATTCGCT GGATCCTGTC AATTGGCCTT AAAGAATATG CAAACAATCT TATAGAGAGT 
GGTGTTCACG GAGCACTTCT GGCCTTAGAT GAAACCTTCG ACTTCAGTGC ACTGGCACTG 
CTGTTACAGA TCCCGACGCA GAACACACAG GCTCGTGCTG TCTTGGAAAG AGAATTTAAC 
AACCTTTTGG TCATGGGGAC TGATAGAAGG TTTGATGAAG ATGATGATAA AAGCTTTAGG 
AGAGCACCTT CATGGAGAAA AAAGTTTAGA CCAAAGGACA TTCGTGGCTT AGCTGCTGGG 
TCAGCAGAGA CTCTCCCTGC AAACTTCCGG GTGACTTCTT CTATGTCTTC CCCCTCTATG 
CAGCCAAAGA AGATGCAGAT GGACGGCAAT GTATCAGGAA CACAGAGGTT GGATTCTGCT 
ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543615.1
CDS	229..4032
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545313.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545313.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC TAGTGATTTA AGGAAACATC GTAGAAAGTT GCCACCTTCC 
AGAGAAGAGG TACGAGATGA CAAGACAACC ATAAAGTGTG AAACCTCCCC GCCTTCCTCC 
CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC AAAGGGGCGC TGCACACCGT CAGCCACGAG 
GACATCAGGG ACATAAGGAA CTCCACAGGC TCCCAGGATG GTCCCGTGAG CAACCCCAGC 
AGTAGCAACA GTAGCCAGGA CTCGCTCCAC AAAGCCCCAA AGAAGAAAGG CATTAAGTCC 
TCCATTGGCC GCTTGTTTGG CAAGAAAGAA AAGGGCCGAC CTGGACAAAC TGGCAAAGAA 
GCATTAGGAC AAGCTGGTGT TTCCGAGACG GATAACTCAT CTCAGGATGC CTTGGGACTT 
AGCAAATTGG GGGGACAGGC TGAAAAAAAT CGTAAACTTC AAAAAAAGCA TGAATTGCTG 
GAGGAAGCCC GGAGACAAGG TTTACCTTTT GCCCAATGGG ACGGGCCAAC GGTTGTGGTC 
TGGCTAGAGC TCTGGGTTGG GATGCCAGCC TGGTATGTGG CTGCCTGCCG AGCAAACGTG 
AAAAGCGGGG CCATCATGTC GGCCCTGTCC GACACAGAGA TCCAGCGTGA GATTGGCATC 
AGCAACCCCC TGCACAGGCT GAAGCTGAGG CTGGCCATCC AGGAGATCAT GTCGCTGACC 
AGCCCGTCTG CCCCGCCCAC ATCTAGAACG ACCACAGGAA ATGTCTGGTT AACACACGAA 
GAGATGGAAA CGCTCGCAGC CACGCCGCAA ACGGAAGATG AGGAGGGAAG CTGGGCTCAG 
ACACTCGCCT ATGGGGACAT GAACCACGAG TGGATCGGCA ACGAGTGGCT CCCCAGCCTG 
GGCCTCCCCC AGTACCGCAG CTACTTCATG GAGTGCCTTG TAGACGCCAG GATGCTGGAC 
CACTTGACCA AGAAAGACCT TCGAGGGCAG CTGAAAATGG TCGACAGTTT TCACAGAAAC 
AGTTTCCAGT GTGGAATTAT GTGCCTGAGA AGGTTAAATT ATGACCGGAA AGAACTGGAA 
AGAAAAAGAG AAGAAAGTCA GAGTGAAATA AAAGACGTGC TTGTTTGGAG CAATGATCGA 
GTGATTCGCT GGATCCTGTC AATTGGCCTT AAAGAATATG CAAACAATCT TATAGAGAGT 
GGTGTTCACG GAGCACTTCT GGCCTTAGAT GAAACCTTCG ACTTCAGTGC ACTGGCACTG 
CTGTTACAGA TCCCGACGCA GAACACACAG GCTCGTGCTG TCTTGGAAAG AGAATTTAAC 
AACCTTTTGG TCATGGGGAC TGATAGAAGG TTTGATGAAG ATGATGATAA AAGCTTTAGG 
AGAGCACCTT CATGGAGAAA AAAGTTTAGA CCAAAGGACA TTCGTGGCTT AGCTGCTGGG 
TCAGCAGAGA CTCTCCCTGC AAACTTCCGG GTGACTTCTT CTATGTCTTC CCCCTCTATG 
CAGCCAAAGA AGATGCAGAT GGACGGCAAT GTATCAGGAA CACAGAGGTT GGATTCTGCT 
ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545314.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543616.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.

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CloneID	OHu61868
Clone ID Related Accession (Same CDS sequence)	XM_011545314.3 , XM_011545314.2
Accession Version	XM_011545314.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTTT GCCACCTTCC AGAGAAGAGG TACGAGATGA CAAGACAACC 
ATAAAGTGTG AAACCTCCCC GCCTTCCTCC CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC 
AAAGGGGCGC TGCACACCGT CAGCCACGAG GACATCAGGG ACATAAGGAA CTCCACAGGC 
TCCCAGGATG GTCCCGTGAG CAACCCCAGC AGTAGCAACA GTAGCCAGGA CTCGCTCCAC 
AAAGCCCCAA AGAAGAAAGG CATTAAGTCC TCCATTGGCC GCTTGTTTGG CAAGAAAGAA 
AAGGGCCGAC CTGGACAAAC TGGCAAAGAA GCATTAGGAC AAGCTGGTGT TTCCGAGACG 
GATAACTCAT CTCAGGATGC CTTGGGACTT AGCAAATTGG GGGGACAGGC TGAAAAAAAT 
CGTAAACTTC AAAAAAAGCA TGAATTGCTG GAGGAAGCCC GGAGACAAGG TTTACCTTTT 
GCCCAATGGG ACGGGCCAAC GGTTGTGGTC TGGCTAGAGC TCTGGGTTGG GATGCCAGCC 
TGGTATGTGG CTGCCTGCCG AGCAAACGTG AAAAGCGGGG CCATCATGTC GGCCCTGTCC 
GACACAGAGA TCCAGCGTGA GATTGGCATC AGCAACCCCC TGCACAGGCT GAAGCTGAGG 
CTGGCCATCC AGGAGATCAT GTCGCTGACC AGCCCGTCTG CCCCGCCCAC ATCTAGAACG 
ACCACAGGAA ATGTCTGGTT AACACACGAA GAGATGGAAA CGCTCGCAGC CACGCCGCAA 
ACGGAAGATG AGGAGGGAAG CTGGGCTCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543616.1
CDS	229..4002
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545314.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545314.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTTT GCCACCTTCC AGAGAAGAGG TACGAGATGA CAAGACAACC 
ATAAAGTGTG AAACCTCCCC GCCTTCCTCC CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC 
AAAGGGGCGC TGCACACCGT CAGCCACGAG GACATCAGGG ACATAAGGAA CTCCACAGGC 
TCCCAGGATG GTCCCGTGAG CAACCCCAGC AGTAGCAACA GTAGCCAGGA CTCGCTCCAC 
AAAGCCCCAA AGAAGAAAGG CATTAAGTCC TCCATTGGCC GCTTGTTTGG CAAGAAAGAA 
AAGGGCCGAC CTGGACAAAC TGGCAAAGAA GCATTAGGAC AAGCTGGTGT TTCCGAGACG 
GATAACTCAT CTCAGGATGC CTTGGGACTT AGCAAATTGG GGGGACAGGC TGAAAAAAAT 
CGTAAACTTC AAAAAAAGCA TGAATTGCTG GAGGAAGCCC GGAGACAAGG TTTACCTTTT 
GCCCAATGGG ACGGGCCAAC GGTTGTGGTC TGGCTAGAGC TCTGGGTTGG GATGCCAGCC 
TGGTATGTGG CTGCCTGCCG AGCAAACGTG AAAAGCGGGG CCATCATGTC GGCCCTGTCC 
GACACAGAGA TCCAGCGTGA GATTGGCATC AGCAACCCCC TGCACAGGCT GAAGCTGAGG 
CTGGCCATCC AGGAGATCAT GTCGCTGACC AGCCCGTCTG CCCCGCCCAC ATCTAGAACG 
ACCACAGGAA ATGTCTGGTT AACACACGAA GAGATGGAAA CGCTCGCAGC CACGCCGCAA 
ACGGAAGATG AGGAGGGAAG CTGGGCTCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545314.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543616.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu61868
Clone ID Related Accession (Same CDS sequence)	XM_011545314.3 , XM_011545314.2
Accession Version	XM_011545314.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTTT GCCACCTTCC AGAGAAGAGG TACGAGATGA CAAGACAACC 
ATAAAGTGTG AAACCTCCCC GCCTTCCTCC CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC 
AAAGGGGCGC TGCACACCGT CAGCCACGAG GACATCAGGG ACATAAGGAA CTCCACAGGC 
TCCCAGGATG GTCCCGTGAG CAACCCCAGC AGTAGCAACA GTAGCCAGGA CTCGCTCCAC 
AAAGCCCCAA AGAAGAAAGG CATTAAGTCC TCCATTGGCC GCTTGTTTGG CAAGAAAGAA 
AAGGGCCGAC CTGGACAAAC TGGCAAAGAA GCATTAGGAC AAGCTGGTGT TTCCGAGACG 
GATAACTCAT CTCAGGATGC CTTGGGACTT AGCAAATTGG GGGGACAGGC TGAAAAAAAT 
CGTAAACTTC AAAAAAAGCA TGAATTGCTG GAGGAAGCCC GGAGACAAGG TTTACCTTTT 
GCCCAATGGG ACGGGCCAAC GGTTGTGGTC TGGCTAGAGC TCTGGGTTGG GATGCCAGCC 
TGGTATGTGG CTGCCTGCCG AGCAAACGTG AAAAGCGGGG CCATCATGTC GGCCCTGTCC 
GACACAGAGA TCCAGCGTGA GATTGGCATC AGCAACCCCC TGCACAGGCT GAAGCTGAGG 
CTGGCCATCC AGGAGATCAT GTCGCTGACC AGCCCGTCTG CCCCGCCCAC ATCTAGAACG 
ACCACAGGAA ATGTCTGGTT AACACACGAA GAGATGGAAA CGCTCGCAGC CACGCCGCAA 
ACGGAAGATG AGGAGGGAAG CTGGGCTCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543616.1
CDS	229..4002
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545314.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545314.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTTT GCCACCTTCC AGAGAAGAGG TACGAGATGA CAAGACAACC 
ATAAAGTGTG AAACCTCCCC GCCTTCCTCC CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC 
AAAGGGGCGC TGCACACCGT CAGCCACGAG GACATCAGGG ACATAAGGAA CTCCACAGGC 
TCCCAGGATG GTCCCGTGAG CAACCCCAGC AGTAGCAACA GTAGCCAGGA CTCGCTCCAC 
AAAGCCCCAA AGAAGAAAGG CATTAAGTCC TCCATTGGCC GCTTGTTTGG CAAGAAAGAA 
AAGGGCCGAC CTGGACAAAC TGGCAAAGAA GCATTAGGAC AAGCTGGTGT TTCCGAGACG 
GATAACTCAT CTCAGGATGC CTTGGGACTT AGCAAATTGG GGGGACAGGC TGAAAAAAAT 
CGTAAACTTC AAAAAAAGCA TGAATTGCTG GAGGAAGCCC GGAGACAAGG TTTACCTTTT 
GCCCAATGGG ACGGGCCAAC GGTTGTGGTC TGGCTAGAGC TCTGGGTTGG GATGCCAGCC 
TGGTATGTGG CTGCCTGCCG AGCAAACGTG AAAAGCGGGG CCATCATGTC GGCCCTGTCC 
GACACAGAGA TCCAGCGTGA GATTGGCATC AGCAACCCCC TGCACAGGCT GAAGCTGAGG 
CTGGCCATCC AGGAGATCAT GTCGCTGACC AGCCCGTCTG CCCCGCCCAC ATCTAGAACG 
ACCACAGGAA ATGTCTGGTT AACACACGAA GAGATGGAAA CGCTCGCAGC CACGCCGCAA 
ACGGAAGATG AGGAGGGAAG CTGGGCTCAG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545315.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543617.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu61869
Clone ID Related Accession (Same CDS sequence)	XM_011545315.2
Accession Version	XM_011545315.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCTAGTG ATTTAAGGAA ACATCGTAGA AAGTTGCCAC CTTCCAGAGA AGAGGTACGA 
GATGACAAGA CAACCATAAA GTGTGAAACC TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG 
TTAGACCGGC TGCACAAAGG GGCGCTGCAC ACCGTCAGCC ACGAGGACAT CAGGGACATA 
AGGAACTCCA CAGGCTCCCA GGATGGTCCC GTGAGCAACC CCAGCAGTAG CAACAGTAGC 
CAGGACTCGC TCCACAAAGC CCCAAAGAAG AAAGGCATTA AGTCCTCCAT TGGCCGCTTG 
TTTGGCAAGA AAGAAAAGGG CCGACCTGGA CAAACTGGCA AAGAAGCATT AGGACAAGCT 
GGTGTTTCCG AGACGGATAA CTCATCTCAG GATGCCTTGG GACTTAGCAA ATTGGGGGGA 
CAGGCTGAAA AAAATCGTAA ACTTCAAAAA AAGCATGAAT TGCTGGAGGA AGCCCGGAGA 
CAAGGTTTAC CTTTTGCCCA ATGGGACGGG CCAACGGTTG TGGTCTGGCT AGAGCTCTGG 
GTTGGGATGC CAGCCTGGTA TGTGGCTGCC TGCCGAGCAA ACGTGAAAAG CGGGGCCATC 
ATGTCGGCCC TGTCCGACAC AGAGATCCAG CGTGAGATTG GCATCAGCAA CCCCCTGCAC 
AGGCTGAAGC TGAGGCTGGC CATCCAGGAG ATCATGTCGC TGACCAGCCC GTCTGCCCCG 
CCCACATCTA GAACGACCAC AGGAAATGTC TGGTTAACAC ACGAAGAGAT GGAAACGCTC 
GCAGCCACGC CGCAAACGGA AGATGAGGAG GGAAGCTGGG CTCAGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GCAATGTATC AGGAACACAG AGGTTGGATT CTGCTACAGT CAGGACTTAC 
TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543617.1
CDS	229..3957
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545315.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545315.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCTAGTG ATTTAAGGAA ACATCGTAGA AAGTTGCCAC CTTCCAGAGA AGAGGTACGA 
GATGACAAGA CAACCATAAA GTGTGAAACC TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG 
TTAGACCGGC TGCACAAAGG GGCGCTGCAC ACCGTCAGCC ACGAGGACAT CAGGGACATA 
AGGAACTCCA CAGGCTCCCA GGATGGTCCC GTGAGCAACC CCAGCAGTAG CAACAGTAGC 
CAGGACTCGC TCCACAAAGC CCCAAAGAAG AAAGGCATTA AGTCCTCCAT TGGCCGCTTG 
TTTGGCAAGA AAGAAAAGGG CCGACCTGGA CAAACTGGCA AAGAAGCATT AGGACAAGCT 
GGTGTTTCCG AGACGGATAA CTCATCTCAG GATGCCTTGG GACTTAGCAA ATTGGGGGGA 
CAGGCTGAAA AAAATCGTAA ACTTCAAAAA AAGCATGAAT TGCTGGAGGA AGCCCGGAGA 
CAAGGTTTAC CTTTTGCCCA ATGGGACGGG CCAACGGTTG TGGTCTGGCT AGAGCTCTGG 
GTTGGGATGC CAGCCTGGTA TGTGGCTGCC TGCCGAGCAA ACGTGAAAAG CGGGGCCATC 
ATGTCGGCCC TGTCCGACAC AGAGATCCAG CGTGAGATTG GCATCAGCAA CCCCCTGCAC 
AGGCTGAAGC TGAGGCTGGC CATCCAGGAG ATCATGTCGC TGACCAGCCC GTCTGCCCCG 
CCCACATCTA GAACGACCAC AGGAAATGTC TGGTTAACAC ACGAAGAGAT GGAAACGCTC 
GCAGCCACGC CGCAAACGGA AGATGAGGAG GGAAGCTGGG CTCAGACACT CGCCTATGGG 
GACATGAACC ACGAGTGGAT CGGCAACGAG TGGCTCCCCA GCCTGGGCCT CCCCCAGTAC 
CGCAGCTACT TCATGGAGTG CCTTGTAGAC GCCAGGATGC TGGACCACTT GACCAAGAAA 
GACCTTCGAG GGCAGCTGAA AATGGTCGAC AGTTTTCACA GAAACAGTTT CCAGTGTGGA 
ATTATGTGCC TGAGAAGGTT AAATTATGAC CGGAAAGAAC TGGAAAGAAA AAGAGAAGAA 
AGTCAGAGTG AAATAAAAGA CGTGCTTGTT TGGAGCAATG ATCGAGTGAT TCGCTGGATC 
CTGTCAATTG GCCTTAAAGA ATATGCAAAC AATCTTATAG AGAGTGGTGT TCACGGAGCA 
CTTCTGGCCT TAGATGAAAC CTTCGACTTC AGTGCACTGG CACTGCTGTT ACAGATCCCG 
ACGCAGAACA CACAGGCTCG TGCTGTCTTG GAAAGAGAAT TTAACAACCT TTTGGTCATG 
GGGACTGATA GAAGGTTTGA TGAAGATGAT GATAAAAGCT TTAGGAGAGC ACCTTCATGG 
AGAAAAAAGT TTAGACCAAA GGACATTCGT GGCTTAGCTG CTGGGTCAGC AGAGACTCTC 
CCTGCAAACT TCCGGGTGAC TTCTTCTATG TCTTCCCCCT CTATGCAGCC AAAGAAGATG 
CAGATGGACG GCAATGTATC AGGAACACAG AGGTTGGATT CTGCTACAGT CAGGACTTAC 
TCCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545316.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543618.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X13, mRNA.

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CloneID	OHu61870
Clone ID Related Accession (Same CDS sequence)	XM_011545316.2
Accession Version	XM_011545316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC TAGTGATTTA AGGAAACATC GTAGAAAGTT GCCACCTTCC 
AGAGAAGAGG TACGAGATGA CAAGACAACC ATAAAGTGTG AAACCTCCCC GCCTTCCTCC 
CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC AAAGGGGCGC TGCACACCGT CAGCCACGAG 
GACATCAGGG ACATAAGGAA CTCCACAGGC TCCCAGGATG GTCCCGTGAG CAACCCCAGC 
AGTAGCAACA GTAGCCAGGA CTCGCTCCAC AAAGCCCCAA AGAAGAAAGG CATTAAGTCC 
TCCATTGGCC GCTTGTTTGG CAAGAAAGAA AAGGGCCGAC CTGGACAAAC TGGCAAAGAA 
GCATTAGGAC AAGCTGGTGT TTCCGAGACG GATAACTCAT CTCAGGATGC CTTGGGACTT 
AGCAAATTGG GGGGACAGGC TGAAAAAAAT CGTAAACTTC AAAAAAAGCA TGAATTGCTG 
GAGGAAGCCC GGAGACAAGG TTTACCTTTT GCCCAATGGG ACGGGCCAAC GGTTGTGGTC 
TGGCTAGAGC TCTGGGTTGG GATGCCAGCC TGGTATGTGG CTGCCTGCCG AGCAAACGTG 
AAAAGCGGGG CCATCATGTC GGCCCTGTCC GACACAGAGA TCCAGCGTGA GATTGGCATC 
AGCAACCCCC TGCACAGGCT GAAGCTGAGG CTGGCCATCC AGGAGATCAT GTCGCTGACC 
AGCCCGTCTG CCCCGCCCAC ATCTAGAACG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543618.1
CDS	229..3942
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545316.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545316.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTCC TAGTGATTTA AGGAAACATC GTAGAAAGTT GCCACCTTCC 
AGAGAAGAGG TACGAGATGA CAAGACAACC ATAAAGTGTG AAACCTCCCC GCCTTCCTCC 
CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC AAAGGGGCGC TGCACACCGT CAGCCACGAG 
GACATCAGGG ACATAAGGAA CTCCACAGGC TCCCAGGATG GTCCCGTGAG CAACCCCAGC 
AGTAGCAACA GTAGCCAGGA CTCGCTCCAC AAAGCCCCAA AGAAGAAAGG CATTAAGTCC 
TCCATTGGCC GCTTGTTTGG CAAGAAAGAA AAGGGCCGAC CTGGACAAAC TGGCAAAGAA 
GCATTAGGAC AAGCTGGTGT TTCCGAGACG GATAACTCAT CTCAGGATGC CTTGGGACTT 
AGCAAATTGG GGGGACAGGC TGAAAAAAAT CGTAAACTTC AAAAAAAGCA TGAATTGCTG 
GAGGAAGCCC GGAGACAAGG TTTACCTTTT GCCCAATGGG ACGGGCCAAC GGTTGTGGTC 
TGGCTAGAGC TCTGGGTTGG GATGCCAGCC TGGTATGTGG CTGCCTGCCG AGCAAACGTG 
AAAAGCGGGG CCATCATGTC GGCCCTGTCC GACACAGAGA TCCAGCGTGA GATTGGCATC 
AGCAACCCCC TGCACAGGCT GAAGCTGAGG CTGGCCATCC AGGAGATCAT GTCGCTGACC 
AGCCCGTCTG CCCCGCCCAC ATCTAGAACG ACACTCGCCT ATGGGGACAT GAACCACGAG 
TGGATCGGCA ACGAGTGGCT CCCCAGCCTG GGCCTCCCCC AGTACCGCAG CTACTTCATG 
GAGTGCCTTG TAGACGCCAG GATGCTGGAC CACTTGACCA AGAAAGACCT TCGAGGGCAG 
CTGAAAATGG TCGACAGTTT TCACAGAAAC AGTTTCCAGT GTGGAATTAT GTGCCTGAGA 
AGGTTAAATT ATGACCGGAA AGAACTGGAA AGAAAAAGAG AAGAAAGTCA GAGTGAAATA 
AAAGACGTGC TTGTTTGGAG CAATGATCGA GTGATTCGCT GGATCCTGTC AATTGGCCTT 
AAAGAATATG CAAACAATCT TATAGAGAGT GGTGTTCACG GAGCACTTCT GGCCTTAGAT 
GAAACCTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA TCCCGACGCA GAACACACAG 
GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG TCATGGGGAC TGATAGAAGG 
TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT CATGGAGAAA AAAGTTTAGA 
CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA CTCTCCCTGC AAACTTCCGG 
GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA AGATGCAGAT GGACGGCAAT 
GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545317.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543619.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu61871
Clone ID Related Accession (Same CDS sequence)	XM_011545317.2
Accession Version	XM_011545317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTTT GCCACCTTCC AGAGAAGAGG TACGAGATGA CAAGACAACC 
ATAAAGTGTG AAACCTCCCC GCCTTCCTCC CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC 
AAAGGGGCGC TGCACACCGT CAGCCACGAG GACATCAGGG ACATAAGGAA CTCCACAGGC 
TCCCAGGATG GTCCCGTGAG CAACCCCAGC AGTAGCAACA GTAGCCAGGA CTCGCTCCAC 
AAAGCCCCAA AGAAGAAAGG CATTAAGTCC TCCATTGGCC GCTTGTTTGG CAAGAAAGAA 
AAGGGCCGAC CTGGACAAAC TGGCAAAGAA GCATTAGGAC AAGCTGGTGT TTCCGAGACG 
GATAACTCAT CTCAGGATGC CTTGGGACTT AGCAAATTGG GGGGACAGGC TGAAAAAAAT 
CGTAAACTTC AAAAAAAGCA TGAATTGCTG GAGGAAGCCC GGAGACAAGG TTTACCTTTT 
GCCCAATGGG ACGGGCCAAC GGTTGTGGTC TGGCTAGAGC TCTGGGTTGG GATGCCAGCC 
TGGTATGTGG CTGCCTGCCG AGCAAACGTG AAAAGCGGGG CCATCATGTC GGCCCTGTCC 
GACACAGAGA TCCAGCGTGA GATTGGCATC AGCAACCCCC TGCACAGGCT GAAGCTGAGG 
CTGGCCATCC AGGAGATCAT GTCGCTGACC AGCCCGTCTG CCCCGCCCAC ATCTAGAACG 
ACACTCGCCT ATGGGGACAT GAACCACGAG TGGATCGGCA ACGAGTGGCT CCCCAGCCTG 
GGCCTCCCCC AGTACCGCAG CTACTTCATG GAGTGCCTTG TAGACGCCAG GATGCTGGAC 
CACTTGACCA AGAAAGACCT TCGAGGGCAG CTGAAAATGG TCGACAGTTT TCACAGAAAC 
AGTTTCCAGT GTGGAATTAT GTGCCTGAGA AGGTTAAATT ATGACCGGAA AGAACTGGAA 
AGAAAAAGAG AAGAAAGTCA GAGTGAAATA AAAGACGTGC TTGTTTGGAG CAATGATCGA 
GTGATTCGCT GGATCCTGTC AATTGGCCTT AAAGAATATG CAAACAATCT TATAGAGAGT 
GGTGTTCACG GAGCACTTCT GGCCTTAGAT GAAACCTTCG ACTTCAGTGC ACTGGCACTG 
CTGTTACAGA TCCCGACGCA GAACACACAG GCTCGTGCTG TCTTGGAAAG AGAATTTAAC 
AACCTTTTGG TCATGGGGAC TGATAGAAGG TTTGATGAAG ATGATGATAA AAGCTTTAGG 
AGAGCACCTT CATGGAGAAA AAAGTTTAGA CCAAAGGACA TTCGTGGCTT AGCTGCTGGG 
TCAGCAGAGA CTCTCCCTGC AAACTTCCGG GTGACTTCTT CTATGTCTTC CCCCTCTATG 
CAGCCAAAGA AGATGCAGAT GGACGGCAAT GTATCAGGAA CACAGAGGTT GGATTCTGCT 
ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543619.1
CDS	229..3912
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSDLLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545317.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545317.2

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGTCTGACCT TTTGTCTTCT GGGAGCTCTG CTGCTAAGGA AGCTAAACTG 
TTGGAACTTA CTTCCAAGCT TAGGAAGGCT GAAGAGAGAC ACGGCAACAT TGAAGAAAGG 
TTACGACAGA TGGAAGCACA GTTGGAGGAG AAGAATCAAG AACTGCAGCG GGCAAGGCAA 
AGAGAAAAAA TGAACGAAGA ACATAATAAA CGTTTATCAG ACACTGTTGA CAAGCTGCTT 
TCAGAATCTA ATGAGAGGCT TCAACTTCAT CTTAAAGAGA GAATGGCTGC TTTGGAAGAT 
AAGAACTCTC TTTTAAGAGA AGTTGAAAGT GCAAAAAAGC AGTTAGAAGA AACACAACAC 
GATAAGGATC AGCTTGTCCT AAACATTGAA GCACTGAGGG CTGAACTAGA CCACATGAGA 
CTAAGAGGTG CTTCACTTCA TCATGGCCGA CCCCACTTGG GCAGTGTCCC AGATTTCAGG 
TTCCCCATGG CAGACGGCCA CACAGACTCC TACAGCACCA GTGCAGTGCT GCGGCGCCCA 
CAGAAAGGCC GGCTGGCAGC CCTGCGAGAT GAGCCTTCCA AGGTACAAAC TCTTAATGAG 
CAGGATTGGG AACGTGCCCA GCAAGCTAGT GTCTTGGCAA ATGTAGCACA AGCATTCGAG 
AGTGATGCTG ACGTGTCTGA TGGTGAAGAT GACAGGGACA CTCTCCTCAG CTCAGTTGAC 
CTGCTATCGC CCAGCGGGCA GGCCGACGCG CACACACTAG CCATGATGCT TCAGGAGCAG 
CTGGACGCCA TCAACAAAGA GATCAGGTTG ATTCAGGAAG AAAAAGAAAA TACAGAGCAG 
CGGGCAGAGG AGATTGAAAG TCGAGTTGGC AGTGGAAGTC TAGACAATCT TGGTCGTTTT 
AGATCAATGA GCTCCATTCC CCCCTACCCT GCTTCCTCGC TTGCTAGCTC CTCCCCTCCG 
GGCAGTGGGC GCTCCACCCC ACGAAGGATC CCTCACAGCC CAGCTCGGGA AGTGGACAGA 
CTGGGCGTCA TGACCCTTTT GCCACCTTCC AGAGAAGAGG TACGAGATGA CAAGACAACC 
ATAAAGTGTG AAACCTCCCC GCCTTCCTCC CCGAGAGCCC TTCGGTTAGA CCGGCTGCAC 
AAAGGGGCGC TGCACACCGT CAGCCACGAG GACATCAGGG ACATAAGGAA CTCCACAGGC 
TCCCAGGATG GTCCCGTGAG CAACCCCAGC AGTAGCAACA GTAGCCAGGA CTCGCTCCAC 
AAAGCCCCAA AGAAGAAAGG CATTAAGTCC TCCATTGGCC GCTTGTTTGG CAAGAAAGAA 
AAGGGCCGAC CTGGACAAAC TGGCAAAGAA GCATTAGGAC AAGCTGGTGT TTCCGAGACG 
GATAACTCAT CTCAGGATGC CTTGGGACTT AGCAAATTGG GGGGACAGGC TGAAAAAAAT 
CGTAAACTTC AAAAAAAGCA TGAATTGCTG GAGGAAGCCC GGAGACAAGG TTTACCTTTT 
GCCCAATGGG ACGGGCCAAC GGTTGTGGTC TGGCTAGAGC TCTGGGTTGG GATGCCAGCC 
TGGTATGTGG CTGCCTGCCG AGCAAACGTG AAAAGCGGGG CCATCATGTC GGCCCTGTCC 
GACACAGAGA TCCAGCGTGA GATTGGCATC AGCAACCCCC TGCACAGGCT GAAGCTGAGG 
CTGGCCATCC AGGAGATCAT GTCGCTGACC AGCCCGTCTG CCCCGCCCAC ATCTAGAACG 
ACACTCGCCT ATGGGGACAT GAACCACGAG TGGATCGGCA ACGAGTGGCT CCCCAGCCTG 
GGCCTCCCCC AGTACCGCAG CTACTTCATG GAGTGCCTTG TAGACGCCAG GATGCTGGAC 
CACTTGACCA AGAAAGACCT TCGAGGGCAG CTGAAAATGG TCGACAGTTT TCACAGAAAC 
AGTTTCCAGT GTGGAATTAT GTGCCTGAGA AGGTTAAATT ATGACCGGAA AGAACTGGAA 
AGAAAAAGAG AAGAAAGTCA GAGTGAAATA AAAGACGTGC TTGTTTGGAG CAATGATCGA 
GTGATTCGCT GGATCCTGTC AATTGGCCTT AAAGAATATG CAAACAATCT TATAGAGAGT 
GGTGTTCACG GAGCACTTCT GGCCTTAGAT GAAACCTTCG ACTTCAGTGC ACTGGCACTG 
CTGTTACAGA TCCCGACGCA GAACACACAG GCTCGTGCTG TCTTGGAAAG AGAATTTAAC 
AACCTTTTGG TCATGGGGAC TGATAGAAGG TTTGATGAAG ATGATGATAA AAGCTTTAGG 
AGAGCACCTT CATGGAGAAA AAAGTTTAGA CCAAAGGACA TTCGTGGCTT AGCTGCTGGG 
TCAGCAGAGA CTCTCCCTGC AAACTTCCGG GTGACTTCTT CTATGTCTTC CCCCTCTATG 
CAGCCAAAGA AGATGCAGAT GGACGGCAAT GTATCAGGAA CACAGAGGTT GGATTCTGCT 
ACAGTCAGGA CTTACTCCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_011545319.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543621.1 Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X18, mRNA.

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CloneID	OHu61873
Clone ID Related Accession (Same CDS sequence)	XM_011545319.1
Accession Version	XM_011545319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCTAGTG ATTTAAGGAA ACATCGTAGA AAGTTGCCAC CTTCCAGAGA AGAGGTACGA 
GATGACAAGA CAACCATAAA GTGTGAAACC TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG 
TTAGACCGGC TGCACAAAGG GGCGCTGCAC ACCGTCAGCC ACGAGGACAT CAGGGACATA 
AGGAACTCCA CAGGCTCCCA GGATGGTCCC GTGAGCAACC CCAGCAGTAG CAACAGTAGC 
CAGGACTCGC TCCACAAAGC CCCAAAGAAG AAAGGCATTA AGTCCTCCAT TGGCCGCTTG 
TTTGGCAAGA AAGAAAAGGG CCGACCTGGA CAAACTGGCA AAGAAGCATT AGGACAAGCT 
GGTGTTTCCG AGACGGATAA CTCATCTCAG GATGCCTTGG GACTTAGCAA ATTGGGGGGA 
CAGGCTGAAA AAAATCGTAA ACTTCAAAAA AAGCATGAAT TGCTGGAGGA AGCCCGGAGA 
CAAGGTTTAC CTTTTGCCCA ATGGGACGGG CCAACGGTTG TGGTCTGGCT AGAGCTCTGG 
GTTGGGATGC CAGCCTGGTA TGTGGCTGCC TGCCGAGCAA ACGTGAAAAG CGGGGCCATC 
ATGTCGGCCC TGTCCGACAC AGAGATCCAG CGTGAGATTG GCATCAGCAA CCCCCTGCAC 
AGGCTGAAGC TGAGGCTGGC CATCCAGGAG ATCATGTCGC TGACCAGCCC GTCTGCCCCG 
CCCACATCTA GAACGACCAC AGGAAATGTC TGGTTAACAC ACGAAGAGAT GGAAACGCTC 
GCAGCCACGC CGCAAACGAC ACTCGCCTAT GGGGACATGA ACCACGAGTG GATCGGCAAC 
GAGTGGCTCC CCAGCCTGGG CCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTTGTA 
GACGCCAGGA TGCTGGACCA CTTGACCAAG AAAGACCTTC GAGGGCAGCT GAAAATGGTC 
GACAGTTTTC ACAGAAACAG TTTCCAGTGT GGAATTATGT GCCTGAGAAG GTTAAATTAT 
GACCGGAAAG AACTGGAAAG AAAAAGAGAA GAAAGTCAGA GTGAAATAAA AGACGTGCTT 
GTTTGGAGCA ATGATCGAGT GATTCGCTGG ATCCTGTCAA TTGGCCTTAA AGAATATGCA 
AACAATCTTA TAGAGAGTGG TGTTCACGGA GCACTTCTGG CCTTAGATGA AACCTTCGAC 
TTCAGTGCAC TGGCACTGCT GTTACAGATC CCGACGCAGA ACACACAGGC TCGTGCTGTC 
TTGGAAAGAG AATTTAACAA CCTTTTGGTC ATGGGGACTG ATAGAAGGTT TGATGAAGAT 
GATGATAAAA GCTTTAGGAG AGCACCTTCA TGGAGAAAAA AGTTTAGACC AAAGGACATT 
CGTGGCTTAG CTGCTGGGTC AGCAGAGACT CTCCCTGCAA ACTTCCGGGT GACTTCTTCT 
ATGTCTTCCC CCTCTATGCA GCCAAAGAAG ATGCAGATGG ACGGCAATGT ATCAGGAACA 
CAGAGGTTGG ATTCTGCTAC AGTCAGGACT TACTCCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543621.1
CDS	229..3930
Translation	MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLPSDLRKHRRKLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLAATPQTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_011545319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545319.1

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC GACCATCAGC GAAGCAGAAG GCCCCCCTGG AGGAGGTGGA 
GGCCATGGTT CCGGCTCCCC TTCACAGCCA GATGCAGATT CACATTTTGA ACAGTTGATG 
GTCTCCATGC TAGAAGAAAG GGACCGCCTT CTTGATACAC TGAGAGAGAC TCAAGAAACG 
CTGGCCTTAA CCCAGGGGAA GTTACACGAG GTTGGTCATG AAAGAGATTC CTTGCAGAGA 
CAGCTCAACA CGGCACTTCC ACAGGAGTTC GCAGCACTTA CTAAAGAACT CAATGTATGC 
AGGGAACAGC TCCTTGAAAG GGAAGAAGAA ATTGCTGAAC TGAAAGCAGA AAGGAATAAC 
ACCAGGCTGC TGTTAGAGCA TTTGGAATGC CTTGTCTCCA GGCATGAGCG GTCTCTTAGG 
ATGACCGTGG TGAAGAGACA AGCGCAGTCT CCAGCAGGCG TGTCCAGCGA AGTGGAAGTG 
CTGAAAGCAC TGAAGTCCTT ATTTGAACAC CACAAAGCTC TGGATGAAAA GGTGAGAGAG 
CGATTACGAG TAGCACTTGA AAGATGTAGT TTGTTAGAAG AGGAATTAGG TGCCACACAC 
AAAGAGCTAA TGATTCTTAA AGAACAGAAT AATCAGAAAA AAACTCTAAC AGATGGAGTG 
CTGGACATAA ACCATGAACA AGAAAATACA CCAAGCACGA GTGGAAAGAG ATCTTCTGAT 
GGTTCTTTAA GCCACGAGGA AGACCTTGCT AAAGTAATTG AGCTCCAAGA AATCATAAGT 
AAGCAGTCAA GGGAACAGAG CCAAATGAAA GAACGCCTGG CTTCCCTTTC CAGTCATGTG 
ACAGAACTGG AAGAGGATCT GGACACGGCT AGAAAAGATC TCATCAAATC TGAAGAAATG 
AACACAAAAT TGCAACGAGA TGTCCGTGAA GCCATGGCCC AAAAGGAAGA TATGGAAGAG 
AGAATCACTA CTCTTGAAAA ACGCTACCTC GCTGCACAGC GTGAAGCCAC ATCTGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCAAATAAAG ATTCTATGCA TCGACAGACT 
GAAGATAAAA ACCGCCAGTT ACAGGAGCGC TTGGAATTGG CAGAGCAAAA GCTGCAACAG 
ACACTGAGGA AGGCAGAGAC GCTCCCGGAG GTGGAGGCGG AGCTGGCCCA GAGGGTGGCA 
GCGCTTTCCA AGGCTGAAGA GAGACACGGC AACATTGAAG AAAGGTTACG ACAGATGGAA 
GCACAGTTGG AGGAGAAGAA TCAAGAACTG CAGCGGGCAA GGCAAAGAGA AAAAATGAAC 
GAAGAACATA ATAAACGTTT ATCAGACACT GTTGACAAGC TGCTTTCAGA ATCTAATGAG 
AGGCTTCAAC TTCATCTTAA AGAGAGAATG GCTGCTTTGG AAGATAAGAA CTCTCTTTTA 
AGAGAAGTTG AAAGTGCAAA AAAGCAGTTA GAAGAAACAC AACACGATAA GGATCAGCTT 
GTCCTAAACA TTGAAGCACT GAGGGCTGAA CTAGACCACA TGAGACTAAG AGGTGCTTCA 
CTTCATCATG GCCGACCCCA CTTGGGCAGT GTCCCAGATT TCAGGTTCCC CATGGCAGAC 
GGCCACACAG ACTCCTACAG CACCAGTGCA GTGCTGCGGC GCCCACAGAA AGGCCGGCTG 
GCAGCCCTGC GAGATGAGCC TTCCAAGGTA CAAACTCTTA ATGAGCAGGA TTGGGAACGT 
GCCCAGCAAG CTAGTGTCTT GGCAAATGTA GCACAAGCAT TCGAGAGTGA TGCTGACGTG 
TCTGATGGTG AAGATGACAG GGACACTCTC CTCAGCTCAG TTGACCTGCT ATCGCCCAGC 
GGGCAGGCCG ACGCGCACAC ACTAGCCATG ATGCTTCAGG AGCAGCTGGA CGCCATCAAC 
AAAGAGATCA GGTTGATTCA GGAAGAAAAA GAAAATACAG AGCAGCGGGC AGAGGAGATT 
GAAAGTCGAG TTGGCAGTGG AAGTCTAGAC AATCTTGGTC GTTTTAGATC AATGAGCTCC 
ATTCCCCCCT ACCCTGCTTC CTCGCTTGCT AGCTCCTCCC CTCCGGGCAG TGGGCGCTCC 
ACCCCACGAA GGATCCCTCA CAGCCCAGCT CGGGAAGTGG ACAGACTGGG CGTCATGACC 
CTTCCTAGTG ATTTAAGGAA ACATCGTAGA AAGTTGCCAC CTTCCAGAGA AGAGGTACGA 
GATGACAAGA CAACCATAAA GTGTGAAACC TCCCCGCCTT CCTCCCCGAG AGCCCTTCGG 
TTAGACCGGC TGCACAAAGG GGCGCTGCAC ACCGTCAGCC ACGAGGACAT CAGGGACATA 
AGGAACTCCA CAGGCTCCCA GGATGGTCCC GTGAGCAACC CCAGCAGTAG CAACAGTAGC 
CAGGACTCGC TCCACAAAGC CCCAAAGAAG AAAGGCATTA AGTCCTCCAT TGGCCGCTTG 
TTTGGCAAGA AAGAAAAGGG CCGACCTGGA CAAACTGGCA AAGAAGCATT AGGACAAGCT 
GGTGTTTCCG AGACGGATAA CTCATCTCAG GATGCCTTGG GACTTAGCAA ATTGGGGGGA 
CAGGCTGAAA AAAATCGTAA ACTTCAAAAA AAGCATGAAT TGCTGGAGGA AGCCCGGAGA 
CAAGGTTTAC CTTTTGCCCA ATGGGACGGG CCAACGGTTG TGGTCTGGCT AGAGCTCTGG 
GTTGGGATGC CAGCCTGGTA TGTGGCTGCC TGCCGAGCAA ACGTGAAAAG CGGGGCCATC 
ATGTCGGCCC TGTCCGACAC AGAGATCCAG CGTGAGATTG GCATCAGCAA CCCCCTGCAC 
AGGCTGAAGC TGAGGCTGGC CATCCAGGAG ATCATGTCGC TGACCAGCCC GTCTGCCCCG 
CCCACATCTA GAACGACCAC AGGAAATGTC TGGTTAACAC ACGAAGAGAT GGAAACGCTC 
GCAGCCACGC CGCAAACGAC ACTCGCCTAT GGGGACATGA ACCACGAGTG GATCGGCAAC 
GAGTGGCTCC CCAGCCTGGG CCTCCCCCAG TACCGCAGCT ACTTCATGGA GTGCCTTGTA 
GACGCCAGGA TGCTGGACCA CTTGACCAAG AAAGACCTTC GAGGGCAGCT GAAAATGGTC 
GACAGTTTTC ACAGAAACAG TTTCCAGTGT GGAATTATGT GCCTGAGAAG GTTAAATTAT 
GACCGGAAAG AACTGGAAAG AAAAAGAGAA GAAAGTCAGA GTGAAATAAA AGACGTGCTT 
GTTTGGAGCA ATGATCGAGT GATTCGCTGG ATCCTGTCAA TTGGCCTTAA AGAATATGCA 
AACAATCTTA TAGAGAGTGG TGTTCACGGA GCACTTCTGG CCTTAGATGA AACCTTCGAC 
TTCAGTGCAC TGGCACTGCT GTTACAGATC CCGACGCAGA ACACACAGGC TCGTGCTGTC 
TTGGAAAGAG AATTTAACAA CCTTTTGGTC ATGGGGACTG ATAGAAGGTT TGATGAAGAT 
GATGATAAAA GCTTTAGGAG AGCACCTTCA TGGAGAAAAA AGTTTAGACC AAAGGACATT 
CGTGGCTTAG CTGCTGGGTC AGCAGAGACT CTCCCTGCAA ACTTCCGGGT GACTTCTTCT 
ATGTCTTCCC CCTCTATGCA GCCAAAGAAG ATGCAGATGG ACGGCAATGT ATCAGGAACA 
CAGAGGTTGG ATTCTGCTAC AGTCAGGACT TACTCCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a membrane-less compartment regulating invadosome function and motility.
Scientific reports8(1)1164(2018 Jan)
Sala K,Raimondi A,Tonoli D,Tacchetti C,de Curtis I

PPFIA1 is upregulated in liver metastasis of breast cancer and is a potential poor prognostic indicator of metastatic relapse.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(7)1010428317713492(2017 Jul)
Yang J,Wu NN,Huang DJ,Luo YC,Huang JZ,He HY,Lu HL,Song WL

PPFIA1 drives active ?5?1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
Nature communications713546(2016 Nov)
Mana G,Clapero F,Panieri E,Panero V,B?ttcher RT,Tseng HY,Saltarin F,Astanina E,Wolanska KI,Morgan MR,Humphries MJ,Santoro MM,Serini G,Valdembri D

Liprin-?1 is a regulator of vimentin intermediate filament network in the cancer cell adhesion machinery.
Scientific reports624486(2016 Apr)
Pehkonen H,von Nandelstadh P,Karhemo PR,Lepikhova T,Grenman R,Lehti K,Monni O

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PPFIA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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