MIXL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIXL1
Entrez Gene ID	83881
Full Name	Mix paired-like homeobox
Synonyms	MILD1,MIX,MIXL
General protein information	Preferred Names Mix paired-like homeobox Names homeobox protein MIXL1 MIX1 homeobox-like protein 1 Mix-like homeobox protein 1 homeodomain protein MIX mix.1 homeobox-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.12
Summary	Homeodomain proteins, such as MIXL1, are transcription factors that regulate cell fate during development (Hart et al., 2005 [PubMed 15982639]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609852

mRNA and Protein(s)

mRNA	Protein	Name
NM_031944.2	NP_114150.1	homeobox protein MIXL1 isoform 2
NM_001282402.2	NP_001269331.1	homeobox protein MIXL1 isoform 1
NM_031944.3	NP_114150.1	homeobox protein MIXL1 isoform 2
NM_001282402.1	NP_001269331.1	homeobox protein MIXL1 isoform 1

Pathways from BioSystems

Homologies

	MIXL1	XP_001091234.1
Pan troglodytes (chimpanzee)	MIXL1	XP_003308838.1
Bos taurus (cattle)	MIXL1	XP_002694024.2
Mus musculus (house mouse)	Mixl1	NP_038757.1
Homo sapiens (human)	MIXL1	NP_114150.1

Gene Ontology
Bibliography

Related articles in PubMed

Mixl1 and Flk1 Are Key Players of Wnt/TGF-? Signaling During DMSO-Induced Mesodermal Specification in P19 cells.
Choi SC, Choi JH, Cui LH, Seo HR, Kim JH, Park CY, Joo HJ, Park JH, Hong SJ, Yu CW, Lim DS
Journal of cellular physiology230(8)1807-21(2015 Aug)

A role for BMP-induced homeobox gene MIXL1 in acute myelogenous leukemia and identification of type I BMP receptor as a potential target for therapy.
Raymond A, Liu B, Liang H, Wei C, Guindani M, Lu Y, Liang S, St John LS, Molldrem J, Nagarajan L
Oncotarget5(24)12675-93(2014 Dec)

Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.
Pereira LA, Wong MS, Lim SM, Sides A, Stanley EG, Elefanty AG
PloS one6(12)e28394(2011)

A census of human transcription factors: function, expression and evolution.
Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM
Nature reviews. Genetics10(4)252-63(2009 04)

Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors.
Davis RP, Ng ES, Costa M, Mossman AK, Sourris K, Elefanty AG, Stanley EG
Blood111(4)1876-84(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that Mixl1 and Flk1 play roles...during dimethyl sulfoxide-induced mesodermal specification in P19 cells.
Title: Mixl1 and Flk1 Are Key Players of Wnt/TGF-? Signaling During DMSO-Induced Mesodermal Specification in P19 cells.

Findings support the existence of a novel MIXL1-c REL mediated survival axis in AML that can be targeted by BMPR1 inhibitors. (MIXL1- human gene, Mixl1- mouse ortholog, MIXL1- protein).
Title: A role for BMP-induced homeobox gene MIXL1 in acute myelogenous leukemia and identification of type I BMP receptor as a potential target for therapy.

Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity
Title: Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.

Targeted insertion in human embryonic stem cells of sequences encoding green fluorescent protein (GFP) into the locus of MIXL1, a gene transiently expressed in the primitive streak during embryogenesis.
Title: Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors.

MIXL1 protein is differentially expressed in non-Hodgkin lymphoma and Hodgkin lymphoma; findings suggest that MIXL1 overexpression may play a role in driving proliferatin or blocking differentiation in lymphoma oncogenesis
Title: Differential expression of the human MIXL1 gene product in non-Hodgkin and Hodgkin lymphomas.

The following MIXL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIXL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01198	NM_031944.3 Latest version!	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu01633	NM_001282402.2 Latest version!	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu01198	NM_031944.2	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu01633	NM_001282402.1	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIXL1 ( NM_031944.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_114150.1 Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01198
Clone ID Related Accession (Same CDS sequence)	NM_031944.2 , NM_031944.3
Accession Version	NM_031944.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	homeobox protein MIXL1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC111974.1, AL592045.28 and CX165667.1. On Sep 5, 2013 this sequence version replaced NM_031944.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC111974.1, SRR1803616.83518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGGTATGGT TCCAGAACAG GCGTGCCAAG 
TCTCGGCGTC AGAGTGGGAA ATCCTTCCAA CCTTTGGCTA GGCCGGAGAT TATCCTCAAC 
CACTGTGCTC CTGGAACTGA AACGAAATGT CTGAAGCCCC AGCTGCCTCT TGAGGTAGAT 
GTGAACTGCC TGCCCGAACC AAACGGGGTT GGAGGGGGCA TCTCTGACTC TAGCTCCCAA 
GGTCAGAATT TTGAAACCTG TTCCCCTCTC TCTGAAGACA TTGGTTCAAA GCTGGACTCA 
TGGGAGGAAC ACATCTTTTC TGCCTTTGGT AACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114150.1
CDS	65..763
Translation	MATAESRALQFAEGAAFPAYRAPHAGGALLPPPSPAAALLPAPPAGPGPATFAGFLGRDPGPAPPPPASLGSPAPPKGAAAPSASQRRKRTSFSAEQLQLLELVFRRTRYPDIHLRERLAALTLLPESRIQVWFQNRRAKSRRQSGKSFQPLARPEIILNHCAPGTETKCLKPQLPLEVDVNCLPEPNGVGGGISDSSSQGQNFETCSPLSEDIGSKLDSWEEHIFSAFGNF

Target ORF information:

RefSeq Version	NM_031944.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031944.2

ORF Insert Sequence:

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGGTATGGT TCCAGAACAG GCGTGCCAAG 
TCTCGGCGTC AGAGTGGGAA ATCCTTCCAA CCTTTGGCTA GGCCGGAGAT TATCCTCAAC 
CACTGTGCTC CTGGAACTGA AACGAAATGT CTGAAGCCCC AGCTGCCTCT TGAGGTAGAT 
GTGAACTGCC TGCCCGAACC AAACGGGGTT GGAGGGGGCA TCTCTGACTC TAGCTCCCAA 
GGTCAGAATT TTGAAACCTG TTCCCCTCTC TCTGAAGACA TTGGTTCAAA GCTGGACTCA 
TGGGAGGAAC ACATCTTTTC TGCCTTTGGT AACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIXL1 ( NM_031944.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_114150.1 Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01198
Clone ID Related Accession (Same CDS sequence)	NM_031944.2 , NM_031944.3
Accession Version	NM_031944.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	homeobox protein MIXL1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC111974.1, AL592045.28 and CX165667.1. On Jun 2, 2019 this sequence version replaced NM_031944.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.83518.1, SRR1803614.461543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGGTATGGT TCCAGAACAG GCGTGCCAAG 
TCTCGGCGTC AGAGTGGGAA ATCCTTCCAA CCTTTGGCTA GGCCGGAGAT TATCCTCAAC 
CACTGTGCTC CTGGAACTGA AACGAAATGT CTGAAGCCCC AGCTGCCTCT TGAGGTAGAT 
GTGAACTGCC TGCCCGAACC AAACGGGGTT GGAGGGGGCA TCTCTGACTC TAGCTCCCAA 
GGTCAGAATT TTGAAACCTG TTCCCCTCTC TCTGAAGACA TTGGTTCAAA GCTGGACTCA 
TGGGAGGAAC ACATCTTTTC TGCCTTTGGT AACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114150.1
CDS	19..717
Translation	MATAESRALQFAEGAAFPAYRAPHAGGALLPPPSPAAALLPAPPAGPGPATFAGFLGRDPGPAPPPPASLGSPAPPKGAAAPSASQRRKRTSFSAEQLQLLELVFRRTRYPDIHLRERLAALTLLPESRIQVWFQNRRAKSRRQSGKSFQPLARPEIILNHCAPGTETKCLKPQLPLEVDVNCLPEPNGVGGGISDSSSQGQNFETCSPLSEDIGSKLDSWEEHIFSAFGNF

Target ORF information:

RefSeq Version	NM_031944.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031944.3

ORF Insert Sequence:

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGGTATGGT TCCAGAACAG GCGTGCCAAG 
TCTCGGCGTC AGAGTGGGAA ATCCTTCCAA CCTTTGGCTA GGCCGGAGAT TATCCTCAAC 
CACTGTGCTC CTGGAACTGA AACGAAATGT CTGAAGCCCC AGCTGCCTCT TGAGGTAGAT 
GTGAACTGCC TGCCCGAACC AAACGGGGTT GGAGGGGGCA TCTCTGACTC TAGCTCCCAA 
GGTCAGAATT TTGAAACCTG TTCCCCTCTC TCTGAAGACA TTGGTTCAAA GCTGGACTCA 
TGGGAGGAAC ACATCTTTTC TGCCTTTGGT AACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIXL1 ( NM_001282402.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269331.1 Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01633
Clone ID Related Accession (Same CDS sequence)	NM_001282402.2 , NM_001282402.1
Accession Version	NM_001282402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	homeobox protein MIXL1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC143784.1, AL592045.28 and CX165667.1. On Jun 2, 2019 this sequence version replaced NM_001282402.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC143784.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGCTTTTAT TTTCTCCCCT CTTCCAGGTA 
TGGTTCCAGA ACAGGCGTGC CAAGTCTCGG CGTCAGAGTG GGAAATCCTT CCAACCTTTG 
GCTAGGCCGG AGATTATCCT CAACCACTGT GCTCCTGGAA CTGAAACGAA ATGTCTGAAG 
CCCCAGCTGC CTCTTGAGGT AGATGTGAAC TGCCTGCCCG AACCAAACGG GGTTGGAGGG 
GGCATCTCTG ACTCTAGCTC CCAAGGTCAG AATTTTGAAA CCTGTTCCCC TCTCTCTGAA 
GACATTGGTT CAAAGCTGGA CTCATGGGAG GAACACATCT TTTCTGCCTT TGGTAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269331.1
CDS	19..741
Translation	MATAESRALQFAEGAAFPAYRAPHAGGALLPPPSPAAALLPAPPAGPGPATFAGFLGRDPGPAPPPPASLGSPAPPKGAAAPSASQRRKRTSFSAEQLQLLELVFRRTRYPDIHLRERLAALTLLPESRIQLLFSPLFQVWFQNRRAKSRRQSGKSFQPLARPEIILNHCAPGTETKCLKPQLPLEVDVNCLPEPNGVGGGISDSSSQGQNFETCSPLSEDIGSKLDSWEEHIFSAFGNF

Target ORF information:

RefSeq Version	NM_001282402.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282402.2

ORF Insert Sequence:

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGCTTTTAT TTTCTCCCCT CTTCCAGGTA 
TGGTTCCAGA ACAGGCGTGC CAAGTCTCGG CGTCAGAGTG GGAAATCCTT CCAACCTTTG 
GCTAGGCCGG AGATTATCCT CAACCACTGT GCTCCTGGAA CTGAAACGAA ATGTCTGAAG 
CCCCAGCTGC CTCTTGAGGT AGATGTGAAC TGCCTGCCCG AACCAAACGG GGTTGGAGGG 
GGCATCTCTG ACTCTAGCTC CCAAGGTCAG AATTTTGAAA CCTGTTCCCC TCTCTCTGAA 
GACATTGGTT CAAAGCTGGA CTCATGGGAG GAACACATCT TTTCTGCCTT TGGTAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIXL1 ( NM_001282402.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269331.1 Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01633
Clone ID Related Accession (Same CDS sequence)	NM_001282402.2 , NM_001282402.1
Accession Version	NM_001282402.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	homeobox protein MIXL1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC143784.1, AL592045.28 and CX165667.1. On Sep 5, 2013 this sequence version replaced XM_005273283.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC143784.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGCTTTTAT TTTCTCCCCT CTTCCAGGTA 
TGGTTCCAGA ACAGGCGTGC CAAGTCTCGG CGTCAGAGTG GGAAATCCTT CCAACCTTTG 
GCTAGGCCGG AGATTATCCT CAACCACTGT GCTCCTGGAA CTGAAACGAA ATGTCTGAAG 
CCCCAGCTGC CTCTTGAGGT AGATGTGAAC TGCCTGCCCG AACCAAACGG GGTTGGAGGG 
GGCATCTCTG ACTCTAGCTC CCAAGGTCAG AATTTTGAAA CCTGTTCCCC TCTCTCTGAA 
GACATTGGTT CAAAGCTGGA CTCATGGGAG GAACACATCT TTTCTGCCTT TGGTAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269331.1
CDS	65..787
Translation	MATAESRALQFAEGAAFPAYRAPHAGGALLPPPSPAAALLPAPPAGPGPATFAGFLGRDPGPAPPPPASLGSPAPPKGAAAPSASQRRKRTSFSAEQLQLLELVFRRTRYPDIHLRERLAALTLLPESRIQLLFSPLFQVWFQNRRAKSRRQSGKSFQPLARPEIILNHCAPGTETKCLKPQLPLEVDVNCLPEPNGVGGGISDSSSQGQNFETCSPLSEDIGSKLDSWEEHIFSAFGNF

Target ORF information:

RefSeq Version	NM_001282402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Mix paired-like homeobox (MIXL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282402.1

ORF Insert Sequence:

ATGGCCACAG CCGAGTCCCG TGCGCTCCAG TTTGCCGAGG GCGCCGCGTT TCCAGCGTAC 
CGGGCCCCCC ACGCCGGCGG GGCGCTCCTG CCGCCCCCGA GCCCTGCGGC AGCCCTGCTC 
CCTGCGCCGC CCGCGGGCCC CGGCCCAGCG ACCTTTGCGG GCTTCCTCGG CCGGGACCCC 
GGGCCGGCCC CGCCGCCCCC CGCCAGCCTG GGCTCGCCTG CGCCCCCCAA AGGCGCGGCC 
GCCCCGTCGG CGTCGCAGCG CCGCAAGCGC ACGTCTTTCA GCGCCGAACA GCTGCAGCTG 
CTGGAGCTCG TCTTCCGCCG GACCCGGTAC CCCGACATCC ACTTGCGCGA GCGCCTGGCC 
GCGCTCACCC TGCTCCCCGA GTCCAGGATC CAGCTTTTAT TTTCTCCCCT CTTCCAGGTA 
TGGTTCCAGA ACAGGCGTGC CAAGTCTCGG CGTCAGAGTG GGAAATCCTT CCAACCTTTG 
GCTAGGCCGG AGATTATCCT CAACCACTGT GCTCCTGGAA CTGAAACGAA ATGTCTGAAG 
CCCCAGCTGC CTCTTGAGGT AGATGTGAAC TGCCTGCCCG AACCAAACGG GGTTGGAGGG 
GGCATCTCTG ACTCTAGCTC CCAAGGTCAG AATTTTGAAA CCTGTTCCCC TCTCTCTGAA 
GACATTGGTT CAAAGCTGGA CTCATGGGAG GAACACATCT TTTCTGCCTT TGGTAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mixl1 and Flk1 Are Key Players of Wnt/TGF-? Signaling During DMSO-Induced Mesodermal Specification in P19 cells.
Journal of cellular physiology230(8)1807-21(2015 Aug)
Choi SC,Choi JH,Cui LH,Seo HR,Kim JH,Park CY,Joo HJ,Park JH,Hong SJ,Yu CW,Lim DS

A role for BMP-induced homeobox gene MIXL1 in acute myelogenous leukemia and identification of type I BMP receptor as a potential target for therapy.
Oncotarget5(24)12675-93(2014 Dec)
Raymond A,Liu B,Liang H,Wei C,Guindani M,Lu Y,Liang S,St John LS,Molldrem J,Nagarajan L

Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.
PloS one6(12)e28394(2011)
Pereira LA,Wong MS,Lim SM,Sides A,Stanley EG,Elefanty AG

A census of human transcription factors: function, expression and evolution.
Nature reviews. Genetics10(4)252-63(2009 04)
Vaquerizas JM,Kummerfeld SK,Teichmann SA,Luscombe NM

Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors.
Blood111(4)1876-84(2008 Feb)
Davis RP,Ng ES,Costa M,Mossman AK,Sourris K,Elefanty AG,Stanley EG