NDFIP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDFIP1
Entrez Gene ID	80762
Full Name	Nedd4 family interacting protein 1
Synonyms	N4WBP5
General protein information	Preferred Names Nedd4 family interacting protein 1 Names NEDD4 family-interacting protein 1 Nedd4 WW domain-binding protein 5 breast cancer-associated protein SGA-1M putative MAPK-activating protein PM13 putative NF-kappa-B-activating protein 164 putative NFKB and MAPK-activating protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008].
Disorder MIM:	612050

mRNA and Protein(s)

mRNA	Protein	Name
NM_030571.3	NP_085048.1	NEDD4 family-interacting protein 1
NM_030571.4	NP_085048.1	NEDD4 family-interacting protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NDFIP1	XP_002689314.3
Drosophila melanogaster (fruit fly)	Ndfip	NP_730291.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005801	XP_001237719.2
Xenopus tropicalis (tropical clawed frog)	ndfip1	NP_001027505.1
Canis lupus familiaris (dog)	NDFIP1	XP_005617965.1
	NDFIP1	XP_001095360.1
Mus musculus (house mouse)	Ndfip1	NP_075372.1
Rattus norvegicus (Norway rat)	Ndfip1	NP_001013077.1
Danio rerio (zebrafish)	ndfip1	NP_955960.1
Homo sapiens (human)	NDFIP1	NP_085048.1
Pan troglodytes (chimpanzee)	NDFIP1	XP_001153016.2

Gene Ontology
Bibliography

Related articles in PubMed

MiR-155 Promotes Uveal Melanoma Cell Proliferation and Invasion by Regulating NDFIP1 Expression.
Peng J, Liu H, Liu C
Technology in cancer research & treatment16(6)1160-1167(2017 Dec)

Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model.
Xing LF, Guo HP, Wang DT, Sun LH, Pan SY
Genetics and molecular research : GMR15(2)(2016 Apr)

Regulation of the human ether-a-go-go-related gene (hERG) potassium channel by Nedd4 family interacting proteins (Ndfips).
Kang Y, Guo J, Yang T, Li W, Zhang S
The Biochemical journal472(1)71-82(2015 Nov)

Nedd4 family interacting protein 1 (Ndfip1) promotes death of pancreatic beta cells.
Beck A, Shatz-Azoulay H, Vinik Y, Isaac R, Boura-Halfon S, Zick Y
Biochemical and biophysical research communications465(4)851-6(2015 Oct)

Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.
Low LH, Chow YL, Li Y, Goh CP, Putz U, Silke J, Ouchi T, Howitt J, Tan SS
The Journal of biological chemistry290(11)7141-50(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the expression of Ndfipl reduced expression of a-synuclein. In conclusion, Ndfipl plays a significant role in protecting SH-SY5Y cells in in vitro Parkinson's disease models.
Title: Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model.

Data show that membrane protein Ndfip1 recruits E3 ubiquitin (Ub) ligase Nedd4-2 to the Golgi to target ether-a-go-go-related gene (hERG) channel for degradation while membrane protein Ndfip2 also mediates Nedd4-2 interaction with hERG in the Golgi.
Title: Regulation of the human ether-a-go-go-related gene (hERG) potassium channel by Nedd4 family interacting proteins (Ndfips).

Silencing of Ndfip1 inhibited cytokine-induced apoptosis of pancreatic islets and promoted glucose-stimulated insulin secretion. These effects were associated with an increase in the cellular content of JunB, a potent inhibitor of ER stress and apoptosis.
Title: Nedd4 family interacting protein 1 (Ndfip1) promotes death of pancreatic beta cells.

Ndfip1 is required during stress for ubiquitinating and trafficking BRAT1 into the nucleus.
Title: Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.

In Parkinson's disease, increased iron levels are associated with increased Ndfip1 expression for the regulation of DMT1, including abnormal Ndfip1 activation in non-neuronal cell types such as astrocytes.
Title: Increased Ndfip1 in the substantia nigra of Parkinsonian brains is associated with elevated iron levels.

The following NDFIP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDFIP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13549	NM_030571.4 Latest version!	Homo sapiens Nedd4 family interacting protein 1 (NDFIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13549	NM_030571.3	Homo sapiens Nedd4 family interacting protein 1 (NDFIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDFIP1 ( NM_030571.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_085048.1 Homo sapiens Nedd4 family interacting protein 1 (NDFIP1), mRNA.

Price & Availability↑

CloneID	OHu13549
Clone ID Related Accession (Same CDS sequence)	NM_030571.3 , NM_030571.4
Accession Version	NM_030571.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-26
Organism	Homo sapiens(human)
Product	NEDD4 family-interacting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236494.1, AB097010.1, AL832993.1 and AA496123.1. On May 21, 2008 this sequence version replaced NM_030571.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.45873.1, SRR1660805.34632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTTGG CGTTGGCGGC GCTGGCGGCG GTCGAGCCGG CCTGCGGCAG CCGGTACCAG 
CAGTTGCAGA ATGAAGAAGA GTCTGGAGAA CCTGAACAGG CTGCAGGTGA TGCTCCTCCA 
CCTTACAGCA GCATTTCTGC AGAGAGCGCA GCATATTTTG ACTACAAGGA TGAGTCTGGG 
TTTCCAAAGC CCCCATCTTA CAATGTAGCT ACAACACTGC CCAGTTATGA TGAAGCGGAG 
AGGACCAAGG CTGAAGCTAC TATCCCTTTG GTTCCTGGGA GAGATGAGGA TTTTGTGGGT 
CGGGATGATT TTGATGATGC TGACCAGCTG AGGATAGGAA ATGATGGGAT TTTCATGTTA 
ACTTTTTTCA TGGCATTCCT CTTTAACTGG ATTGGGTTTT TCCTGTCTTT TTGCCTGACC 
ACTTCAGCTG CAGGAAGGTA TGGGGCCATT TCAGGATTTG GTCTCTCTCT AATTAAATGG 
ATCCTGATTG TCAGGTTTTC CACCTATTTC CCTGGATATT TTGATGGTCA GTACTGGCTC 
TGGTGGGTGT TCCTTGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTATGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085048.1
CDS	217..882
Translation	MALALAALAAVEPACGSRYQQLQNEEESGEPEQAAGDAPPPYSSISAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKAEATIPLVPGRDEDFVGRDDFDDADQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFSNLPRTRVLFIY

Target ORF information:

RefSeq Version	NM_030571.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Nedd4 family interacting protein 1 (NDFIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030571.3

ORF Insert Sequence:

ATGGCGTTGG CGTTGGCGGC GCTGGCGGCG GTCGAGCCGG CCTGCGGCAG CCGGTACCAG 
CAGTTGCAGA ATGAAGAAGA GTCTGGAGAA CCTGAACAGG CTGCAGGTGA TGCTCCTCCA 
CCTTACAGCA GCATTTCTGC AGAGAGCGCA GCATATTTTG ACTACAAGGA TGAGTCTGGG 
TTTCCAAAGC CCCCATCTTA CAATGTAGCT ACAACACTGC CCAGTTATGA TGAAGCGGAG 
AGGACCAAGG CTGAAGCTAC TATCCCTTTG GTTCCTGGGA GAGATGAGGA TTTTGTGGGT 
CGGGATGATT TTGATGATGC TGACCAGCTG AGGATAGGAA ATGATGGGAT TTTCATGTTA 
ACTTTTTTCA TGGCATTCCT CTTTAACTGG ATTGGGTTTT TCCTGTCTTT TTGCCTGACC 
ACTTCAGCTG CAGGAAGGTA TGGGGCCATT TCAGGATTTG GTCTCTCTCT AATTAAATGG 
ATCCTGATTG TCAGGTTTTC CACCTATTTC CCTGGATATT TTGATGGTCA GTACTGGCTC 
TGGTGGGTGT TCCTTGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTATGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDFIP1 ( NM_030571.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_085048.1 Homo sapiens Nedd4 family interacting protein 1 (NDFIP1), mRNA.

Price & Availability↑

CloneID	OHu13549
Clone ID Related Accession (Same CDS sequence)	NM_030571.3 , NM_030571.4
Accession Version	NM_030571.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	NEDD4 family-interacting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236494.1, AB097010.1, AL832993.1 and AA496123.1. On Nov 22, 2018 this sequence version replaced NM_030571.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.136458.1, SRR1660803.45873.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253814.6/ ENSP00000253814.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTTGG CGTTGGCGGC GCTGGCGGCG GTCGAGCCGG CCTGCGGCAG CCGGTACCAG 
CAGTTGCAGA ATGAAGAAGA GTCTGGAGAA CCTGAACAGG CTGCAGGTGA TGCTCCTCCA 
CCTTACAGCA GCATTTCTGC AGAGAGCGCA GCATATTTTG ACTACAAGGA TGAGTCTGGG 
TTTCCAAAGC CCCCATCTTA CAATGTAGCT ACAACACTGC CCAGTTATGA TGAAGCGGAG 
AGGACCAAGG CTGAAGCTAC TATCCCTTTG GTTCCTGGGA GAGATGAGGA TTTTGTGGGT 
CGGGATGATT TTGATGATGC TGACCAGCTG AGGATAGGAA ATGATGGGAT TTTCATGTTA 
ACTTTTTTCA TGGCATTCCT CTTTAACTGG ATTGGGTTTT TCCTGTCTTT TTGCCTGACC 
ACTTCAGCTG CAGGAAGGTA TGGGGCCATT TCAGGATTTG GTCTCTCTCT AATTAAATGG 
ATCCTGATTG TCAGGTTTTC CACCTATTTC CCTGGATATT TTGATGGTCA GTACTGGCTC 
TGGTGGGTGT TCCTTGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTATGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085048.1
CDS	197..862
Translation	MALALAALAAVEPACGSRYQQLQNEEESGEPEQAAGDAPPPYSSISAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKAEATIPLVPGRDEDFVGRDDFDDADQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFSNLPRTRVLFIY

Target ORF information:

RefSeq Version	NM_030571.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Nedd4 family interacting protein 1 (NDFIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030571.4

ORF Insert Sequence:

ATGGCGTTGG CGTTGGCGGC GCTGGCGGCG GTCGAGCCGG CCTGCGGCAG CCGGTACCAG 
CAGTTGCAGA ATGAAGAAGA GTCTGGAGAA CCTGAACAGG CTGCAGGTGA TGCTCCTCCA 
CCTTACAGCA GCATTTCTGC AGAGAGCGCA GCATATTTTG ACTACAAGGA TGAGTCTGGG 
TTTCCAAAGC CCCCATCTTA CAATGTAGCT ACAACACTGC CCAGTTATGA TGAAGCGGAG 
AGGACCAAGG CTGAAGCTAC TATCCCTTTG GTTCCTGGGA GAGATGAGGA TTTTGTGGGT 
CGGGATGATT TTGATGATGC TGACCAGCTG AGGATAGGAA ATGATGGGAT TTTCATGTTA 
ACTTTTTTCA TGGCATTCCT CTTTAACTGG ATTGGGTTTT TCCTGTCTTT TTGCCTGACC 
ACTTCAGCTG CAGGAAGGTA TGGGGCCATT TCAGGATTTG GTCTCTCTCT AATTAAATGG 
ATCCTGATTG TCAGGTTTTC CACCTATTTC CCTGGATATT TTGATGGTCA GTACTGGCTC 
TGGTGGGTGT TCCTTGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTATGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-155 Promotes Uveal Melanoma Cell Proliferation and Invasion by Regulating NDFIP1 Expression.
Technology in cancer research & treatment16(6)1160-1167(2017 Dec)
Peng J,Liu H,Liu C

Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model.
Genetics and molecular research : GMR15(2)(2016 Apr)
Xing LF,Guo HP,Wang DT,Sun LH,Pan SY

Regulation of the human ether-a-go-go-related gene (hERG) potassium channel by Nedd4 family interacting proteins (Ndfips).
The Biochemical journal472(1)71-82(2015 Nov)
Kang Y,Guo J,Yang T,Li W,Zhang S

Nedd4 family interacting protein 1 (Ndfip1) promotes death of pancreatic beta cells.
Biochemical and biophysical research communications465(4)851-6(2015 Oct)
Beck A,Shatz-Azoulay H,Vinik Y,Isaac R,Boura-Halfon S,Zick Y

Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.
The Journal of biological chemistry290(11)7141-50(2015 Mar)
Low LH,Chow YL,Li Y,Goh CP,Putz U,Silke J,Ouchi T,Howitt J,Tan SS