TMBIM4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMBIM4
Entrez Gene ID	51643
Full Name	transmembrane BAX inhibitor motif containing 4
Synonyms	CGI-119,GAAP,LFG4,S1R,ZPRO
General protein information	Preferred Names transmembrane BAX inhibitor motif containing 4 Names protein lifeguard 4 Golgi anti-apoptotic protein Z-protein transmembrane BAX inhibitor motif-containing protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.3
Disorder MIM:	616874

mRNA and Protein(s)

mRNA	Protein	Name
NM_001282609.1	NP_001269538.1	protein lifeguard 4 isoform c
NM_001282610.1	NP_001269539.1	protein lifeguard 4 isoform d
NM_016056.3	NP_057140.2	protein lifeguard 4 isoform b
NM_001282606.1	NP_001269535.1	protein lifeguard 4 isoform a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tmbim4	NP_998303.2
Magnaporthe oryzae (rice blast fungus)	MGG_03089	XP_003720584.1
Rattus norvegicus (Norway rat)	Tmbim4	NP_954547.1
Bos taurus (cattle)	TMBIM4	NP_001014914.1
Neurospora crassa	NCU02463	XP_965057.1
Arabidopsis thaliana (thale cress)	AT4G15470	NP_567466.1
Homo sapiens (human)	TMBIM4	NP_057140.2
Canis lupus familiaris (dog)	TMBIM4	XP_531662.1
Mus musculus (house mouse)	Tmbim4	NP_080893.1
Caenorhabditis elegans (roundworm)	tmbi-4	NP_509543.2
Xenopus tropicalis (tropical clawed frog)	tmbim4	NP_001004879.1
Pan troglodytes (chimpanzee)	TMBIM4	XP_003952181.1
Gallus gallus (chicken)	TMBIM4	XP_001235093.1
Schizosaccharomyces pombe (fission yeast)	bxi1	NP_588431.1
Oryza sativa (rice)	Os05g0402300	NP_001055493.1

Gene Ontology
Bibliography

Related articles in PubMed

Golgi anti-apoptotic protein: a tale of camels, calcium, channels and cancer.
Carrara G, Parsons M, Saraiva N, Smith GL
Open biology7(5)(2017 May)

The transmembrane Bax inhibitor motif (TMBIM) containing protein family: Tissue expression, intracellular localization and effects on the ER CA??-filling state.
Lisak DA, Schacht T, Enders V, Habicht J, Kiviluoto S, Schneider J, Henke N, Bultynck G, Methner A
Biochimica et biophysica acta1853(9)2104-14(2015 Sep)

hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2.
Saraiva N, Prole DL, Carrara G, Johnson BF, Taylor CW, Parsons M, Smith GL
The Journal of cell biology202(4)699-713(2013 Aug)

Human and viral Golgi anti-apoptotic proteins (GAAPs) oligomerize via different mechanisms and monomeric GAAP inhibits apoptosis and modulates calcium.
Saraiva N, Prole DL, Carrara G, Maluquer de Motes C, Johnson BF, Byrne B, Taylor CW, Smith GL
The Journal of biological chemistry288(18)13057-67(2013 May)

Host cell Golgi anti-apoptotic protein (GAAP) and growth of Chlamydia pneumoniae.
Markkula E, Hulkkonen J, Penttil? T, Puolakkainen M
Microbial pathogenesis5446-53(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that the TMBIM family has comparable functions in the maintenance of intracellular Ca(2) homeostasis in a wide variety of tissues
Title: The transmembrane Bax inhibitor motif (TMBIM) containing protein family: Tissue expression, intracellular localization and effects on the ER CA??-filling state.

The hGAAP is a novel regulator of focal adhesion dynamics, cell adhesion, and migration by controlling localized Ca(2+)-dependent activation of calpain.
Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2.

GAAP can oligomerize in a pH-regulated manner, and monomeric GAAP is functional.
Title: Human and viral Golgi anti-apoptotic proteins (GAAPs) oligomerize via different mechanisms and monomeric GAAP inhibits apoptosis and modulates calcium.

Taken together, the proposed interaction between Chlamydia pneumoniae protein CPn0809 and GAAP modulates bacterial growth in A549 cells.
Title: Host cell Golgi anti-apoptotic protein (GAAP) and growth of Chlamydia pneumoniae.

Data indicate that GAAP and BI-1 have a six membrane-spanning topology with cytosolic N and C termini and a C-terminal reentrant loop.
Title: Six-transmembrane topology for Golgi anti-apoptotic protein (GAAP) and Bax inhibitor 1 (BI-1) provides model for the transmembrane Bax inhibitor-containing motif (TMBIM) family.

The following TMBIM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMBIM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09759	NM_001282609.1 Latest version!	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu10033	NM_016056.3 Latest version!	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu10160	NM_001282606.1 Latest version!	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu10161	NM_001282610.1 Latest version!	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMBIM4 ( NM_001282609.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269538.1 Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09759
Clone ID Related Accession (Same CDS sequence)	NM_001282609.1
Accession Version	NM_001282609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	protein lifeguard 4 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC361259.1, BC052273.1, AK225158.1, AF113127.1 and BM989676.1. On Sep 14, 2013 this sequence version replaced XM_005268973.1. Transcript Variant: This variant (3) uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.89238.1, SRR1660807.51710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGACC CCGACCCCCG GTACCCTCGC TCCTCGATCG AGGACGACTT CAACTATGGC 
AGCAGCGTGG CCTCCGCCAC CGTGCACATC CGAATGGCCT TTCTGAGAAA AGTCTACAGC 
ATTCTTTCTC TGCAGGTTCT CTTAACTACA GTGACTTCAA CAGTTTTTTT ATACTTTGAG 
TCTGTACGGA CATTTGTACA TGAGAGTCCT GCCTTAATTT TGCTGTTTGC CCTCGGATCT 
CTGGGTTTGA TTTTTGCGTT GATTTTAAAC AGACATAAGT ATCCCCTTAA CCTGTACCTA 
CTTTTTGGAT TTACGCTGTT GGAAGCTCTG ACTGTGGCAG TTGTTGTTAC TTTCTATGAT 
GTATATATTA TTCTGCAAGC TTTCATACTG ACTACTACAG TATTTTTTGG TTTGACTGTG 
TATACTCTAC AATCTAAGAA GGATTTCAGC AAATTTGGAG CAGGGATTCT TGAAGTTTTT 
TTTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269538.1
CDS	122..610
Translation	MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGILEVFFL

Target ORF information:

RefSeq Version	NM_001282609.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282609.1

ORF Insert Sequence:

ATGGCTGACC CCGACCCCCG GTACCCTCGC TCCTCGATCG AGGACGACTT CAACTATGGC 
AGCAGCGTGG CCTCCGCCAC CGTGCACATC CGAATGGCCT TTCTGAGAAA AGTCTACAGC 
ATTCTTTCTC TGCAGGTTCT CTTAACTACA GTGACTTCAA CAGTTTTTTT ATACTTTGAG 
TCTGTACGGA CATTTGTACA TGAGAGTCCT GCCTTAATTT TGCTGTTTGC CCTCGGATCT 
CTGGGTTTGA TTTTTGCGTT GATTTTAAAC AGACATAAGT ATCCCCTTAA CCTGTACCTA 
CTTTTTGGAT TTACGCTGTT GGAAGCTCTG ACTGTGGCAG TTGTTGTTAC TTTCTATGAT 
GTATATATTA TTCTGCAAGC TTTCATACTG ACTACTACAG TATTTTTTGG TTTGACTGTG 
TATACTCTAC AATCTAAGAA GGATTTCAGC AAATTTGGAG CAGGGATTCT TGAAGTTTTT 
TTTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMBIM4 ( NM_016056.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057140.2 Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10033
Clone ID Related Accession (Same CDS sequence)	NM_016056.3
Accession Version	NM_016056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	protein lifeguard 4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC361259.1, BC020613.1, AF113127.1, AK225158.1 and BM989676.1. On Sep 14, 2013 this sequence version replaced NM_016056.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to vairant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.259252.1, SRR1803611.59735.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGCTGACC CCGACCCCCG GTACCCTCGC TCCTCGATCG AGGACGACTT CAACTATGGC 
AGCAGCGTGG CCTCCGCCAC CGTGCACATC CGAATGGCCT TTCTGAGAAA AGTCTACAGC 
ATTCTTTCTC TGCAGGTTCT CTTAACTACA GTGACTTCAA CAGTTTTTTT ATACTTTGAG 
TCTGTACGGA CATTTGTACA TGAGAGTCCT GCCTTAATTT TGCTGTTTGC CCTCGGATCT 
CTGGGTTTGA TTTTTGCGTT GATTTTAAAC AGACATAAGT ATCCCCTTAA CCTGTACCTA 
CTTTTTGGAT TTACGCTGTT GGAAGCTCTG ACTGTGGCAG TTGTTGTTAC TTTCTATGAT 
GTATATATTA TTCTGCAAGC TTTCATACTG ACTACTACAG TATTTTTTGG TTTGACTGTG 
TATACTCTAC AATCTAAGAA GGATTTCAGC AAATTTGGAG CAGGGCTGTT TGCTCTTTTG 
TGGATATTGT GCCTGTCAGG ATTCTTGAAG TTTTTTTTTT ATAGTGAGAT AATGGAGTTG 
GTCTTAGCCG CTGCAGGAGC CCTTCTTTTC TGTGGATTCA TCATCTATGA CACACACTCA 
CTGATGCATA AACTGTCACC TGAAGAGTAC GTATTAGCTG CCATCAGCCT CTACTTGGAT 
ATCATCAATC TATTCCTGCA CCTGTTACGG TTTCTGGAAG CAGTTAATAA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057140.2
CDS	122..838
Translation	MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCGFIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK

Target ORF information:

RefSeq Version	NM_016056.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016056.3

ORF Insert Sequence:

ATGGCTGACC CCGACCCCCG GTACCCTCGC TCCTCGATCG AGGACGACTT CAACTATGGC 
AGCAGCGTGG CCTCCGCCAC CGTGCACATC CGAATGGCCT TTCTGAGAAA AGTCTACAGC 
ATTCTTTCTC TGCAGGTTCT CTTAACTACA GTGACTTCAA CAGTTTTTTT ATACTTTGAG 
TCTGTACGGA CATTTGTACA TGAGAGTCCT GCCTTAATTT TGCTGTTTGC CCTCGGATCT 
CTGGGTTTGA TTTTTGCGTT GATTTTAAAC AGACATAAGT ATCCCCTTAA CCTGTACCTA 
CTTTTTGGAT TTACGCTGTT GGAAGCTCTG ACTGTGGCAG TTGTTGTTAC TTTCTATGAT 
GTATATATTA TTCTGCAAGC TTTCATACTG ACTACTACAG TATTTTTTGG TTTGACTGTG 
TATACTCTAC AATCTAAGAA GGATTTCAGC AAATTTGGAG CAGGGCTGTT TGCTCTTTTG 
TGGATATTGT GCCTGTCAGG ATTCTTGAAG TTTTTTTTTT ATAGTGAGAT AATGGAGTTG 
GTCTTAGCCG CTGCAGGAGC CCTTCTTTTC TGTGGATTCA TCATCTATGA CACACACTCA 
CTGATGCATA AACTGTCACC TGAAGAGTAC GTATTAGCTG CCATCAGCCT CTACTTGGAT 
ATCATCAATC TATTCCTGCA CCTGTTACGG TTTCTGGAAG CAGTTAATAA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMBIM4 ( NM_001282606.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269535.1 Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10160
Clone ID Related Accession (Same CDS sequence)	NM_001282606.1
Accession Version	NM_001282606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	protein lifeguard 4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC361259.1, BC020613.1, AF182414.1, AF113127.1, AK225158.1 and BM989676.1. On Sep 14, 2013 this sequence version replaced XM_005268969.1. Transcript Variant: This variant (1) represents the longest transcript and enocdes the longest isoform (a). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGACC CCGACCCCCG GTACCCTCGC TCCTCGATCG AGGACGACTT CAACTATGGC 
AGCAGCGTGG CCTCCGCCAC CGTGCACATC CGAATGGTTC TCCAGAGAGA TTGTACCTTC 
AGCAAACAAT ATATGAGGAT ACCTTCTGCA CCCTCAGCCA CACTGAATAT CATCAGATTT 
TTTCATCTCC GGCAATCTGG TAGATCAGGG ATGGTGAACA TTTTCTACAA AGGGCCAACC 
TTTCTGAGAA AAGTCTACAG CATTCTTTCT CTGCAGGTTC TCTTAACTAC AGTGACTTCA 
ACAGTTTTTT TATACTTTGA GTCTGTACGG ACATTTGTAC ATGAGAGTCC TGCCTTAATT 
TTGCTGTTTG CCCTCGGATC TCTGGGTTTG ATTTTTGCGT TGATTTTAAA CAGACATAAG 
TATCCCCTTA ACCTGTACCT ACTTTTTGGA TTTACGCTGT TGGAAGCTCT GACTGTGGCA 
GTTGTTGTTA CTTTCTATGA TGTATATATT ATTCTGCAAG CTTTCATACT GACTACTACA 
GTATTTTTTG GTTTGACTGT GTATACTCTA CAATCTAAGA AGGATTTCAG CAAATTTGGA 
GCAGGGCTGT TTGCTCTTTT GTGGATATTG TGCCTGTCAG GATTCTTGAA GTTTTTTTTT 
TATAGTGAGA TAATGGAGTT GGTCTTAGCC GCTGCAGGAG CCCTTCTTTT CTGTGGATTC 
ATCATCTATG ACACACACTC ACTGATGCAT AAACTGTCAC CTGAAGAGTA CGTATTAGCT 
GCCATCAGCC TCTACTTGGA TATCATCAAT CTATTCCTGC ACCTGTTACG GTTTCTGGAA 
GCAGTTAATA AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269535.1
CDS	122..979
Translation	MADPDPRYPRSSIEDDFNYGSSVASATVHIRMVLQRDCTFSKQYMRIPSAPSATLNIIRFFHLRQSGRSGMVNIFYKGPTFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCGFIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK

Target ORF information:

RefSeq Version	NM_001282606.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282606.1

ORF Insert Sequence:

ATGGCTGACC CCGACCCCCG GTACCCTCGC TCCTCGATCG AGGACGACTT CAACTATGGC 
AGCAGCGTGG CCTCCGCCAC CGTGCACATC CGAATGGTTC TCCAGAGAGA TTGTACCTTC 
AGCAAACAAT ATATGAGGAT ACCTTCTGCA CCCTCAGCCA CACTGAATAT CATCAGATTT 
TTTCATCTCC GGCAATCTGG TAGATCAGGG ATGGTGAACA TTTTCTACAA AGGGCCAACC 
TTTCTGAGAA AAGTCTACAG CATTCTTTCT CTGCAGGTTC TCTTAACTAC AGTGACTTCA 
ACAGTTTTTT TATACTTTGA GTCTGTACGG ACATTTGTAC ATGAGAGTCC TGCCTTAATT 
TTGCTGTTTG CCCTCGGATC TCTGGGTTTG ATTTTTGCGT TGATTTTAAA CAGACATAAG 
TATCCCCTTA ACCTGTACCT ACTTTTTGGA TTTACGCTGT TGGAAGCTCT GACTGTGGCA 
GTTGTTGTTA CTTTCTATGA TGTATATATT ATTCTGCAAG CTTTCATACT GACTACTACA 
GTATTTTTTG GTTTGACTGT GTATACTCTA CAATCTAAGA AGGATTTCAG CAAATTTGGA 
GCAGGGCTGT TTGCTCTTTT GTGGATATTG TGCCTGTCAG GATTCTTGAA GTTTTTTTTT 
TATAGTGAGA TAATGGAGTT GGTCTTAGCC GCTGCAGGAG CCCTTCTTTT CTGTGGATTC 
ATCATCTATG ACACACACTC ACTGATGCAT AAACTGTCAC CTGAAGAGTA CGTATTAGCT 
GCCATCAGCC TCTACTTGGA TATCATCAAT CTATTCCTGC ACCTGTTACG GTTTCTGGAA 
GCAGTTAATA AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMBIM4 ( NM_001282610.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269539.1 Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu10161
Clone ID Related Accession (Same CDS sequence)	NM_001282610.1
Accession Version	NM_001282610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	protein lifeguard 4 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC361259.1, BC004401.1, AF113127.1, AK225158.1 and BM989676.1. Transcript Variant: This variant (4) uses an alternate splice site in the 5' coding region which results in the use of an alternate translational start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.125257.1, SRR1803615.465573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCAG CGTGGCCTCC GCCACCGTGC ACATCCGAAT GGGTTCTCTT AACTACAGTG 
ACTTCAACAG TTTTTTTATA CTTTGAGTCT GTACGGACAT TTGTACATGA GAGTCCTGCC 
TTAATTTTGC TGTTTGCCCT CGGATCTCTG GGTTTGATTT TTGCGTTGAT TTTAAACAGA 
CATAAGTATC CCCTTAACCT GTACCTACTT TTTGGATTTA CGCTGTTGGA AGCTCTGACT 
GTGGCAGTTG TTGTTACTTT CTATGATGTA TATATTATTC TGCAAGCTTT CATACTGACT 
ACTACAGTAT TTTTTGGTTT GACTGTGTAT ACTCTACAAT CTAAGAAGGA TTTCAGCAAA 
TTTGGAGCAG GGCTGTTTGC TCTTTTGTGG ATATTGTGCC TGTCAGGATT CTTGAAGTTT 
TTTTTTTATA GTGAGATAAT GGAGTTGGTC TTAGCCGCTG CAGGAGCCCT TCTTTTCTGT 
GGATTCATCA TCTATGACAC ACACTCACTG ATGCATAAAC TGTCACCTGA AGAGTACGTA 
TTAGCTGCCA TCAGCCTCTA CTTGGATATC ATCAATCTAT TCCTGCACCT GTTACGGTTT 
CTGGAAGCAG TTAATAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269539.1
CDS	177..800
Translation	MAAAWPPPPCTSEWVLLTTVTSTVFLYFESVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCGFIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK

Target ORF information:

RefSeq Version	NM_001282610.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282610.1

ORF Insert Sequence:

ATGGCAGCAG CGTGGCCTCC GCCACCGTGC ACATCCGAAT GGGTTCTCTT AACTACAGTG 
ACTTCAACAG TTTTTTTATA CTTTGAGTCT GTACGGACAT TTGTACATGA GAGTCCTGCC 
TTAATTTTGC TGTTTGCCCT CGGATCTCTG GGTTTGATTT TTGCGTTGAT TTTAAACAGA 
CATAAGTATC CCCTTAACCT GTACCTACTT TTTGGATTTA CGCTGTTGGA AGCTCTGACT 
GTGGCAGTTG TTGTTACTTT CTATGATGTA TATATTATTC TGCAAGCTTT CATACTGACT 
ACTACAGTAT TTTTTGGTTT GACTGTGTAT ACTCTACAAT CTAAGAAGGA TTTCAGCAAA 
TTTGGAGCAG GGCTGTTTGC TCTTTTGTGG ATATTGTGCC TGTCAGGATT CTTGAAGTTT 
TTTTTTTATA GTGAGATAAT GGAGTTGGTC TTAGCCGCTG CAGGAGCCCT TCTTTTCTGT 
GGATTCATCA TCTATGACAC ACACTCACTG ATGCATAAAC TGTCACCTGA AGAGTACGTA 
TTAGCTGCCA TCAGCCTCTA CTTGGATATC ATCAATCTAT TCCTGCACCT GTTACGGTTT 
CTGGAAGCAG TTAATAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Golgi anti-apoptotic protein: a tale of camels, calcium, channels and cancer.
Open biology7(5)(2017 May)
Carrara G,Parsons M,Saraiva N,Smith GL

The transmembrane Bax inhibitor motif (TMBIM) containing protein family: Tissue expression, intracellular localization and effects on the ER CA??-filling state.
Biochimica et biophysica acta1853(9)2104-14(2015 Sep)
Lisak DA,Schacht T,Enders V,Habicht J,Kiviluoto S,Schneider J,Henke N,Bultynck G,Methner A

hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2.
The Journal of cell biology202(4)699-713(2013 Aug)
Saraiva N,Prole DL,Carrara G,Johnson BF,Taylor CW,Parsons M,Smith GL

Human and viral Golgi anti-apoptotic proteins (GAAPs) oligomerize via different mechanisms and monomeric GAAP inhibits apoptosis and modulates calcium.
The Journal of biological chemistry288(18)13057-67(2013 May)
Saraiva N,Prole DL,Carrara G,Maluquer de Motes C,Johnson BF,Byrne B,Taylor CW,Smith GL

Host cell Golgi anti-apoptotic protein (GAAP) and growth of Chlamydia pneumoniae.
Microbial pathogenesis5446-53(2013 Jan)
Markkula E,Hulkkonen J,Penttil? T,Puolakkainen M