PHPT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHPT1
Entrez Gene ID	29085
Full Name	phosphohistidine phosphatase 1
Synonyms	CGI-202,HEL-S-132P,HSPC141,PHP,PHP14
General protein information	Preferred Names phosphohistidine phosphatase 1 Names 14 kDa phosphohistidine phosphatase epididymis secretory sperm binding protein Li 132P protein histidine phosphatase protein janus-A homolog sex-regulated protein janus-a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.3
Summary	This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013].
Disorder MIM:	610167

mRNA and Protein(s)

mRNA	Protein	Name
NM_014172.5	NP_054891.2	14 kDa phosphohistidine phosphatase isoform 3
NM_001135861.2	NP_001129333.1	14 kDa phosphohistidine phosphatase isoform 2
NM_014172.6	NP_054891.2	14 kDa phosphohistidine phosphatase isoform 3
NM_001135861.3	NP_001129333.1	14 kDa phosphohistidine phosphatase isoform 2
NM_001287342.2	NP_001274271.1	14 kDa phosphohistidine phosphatase isoform 4
NM_001287343.1	NP_001274272.1	14 kDa phosphohistidine phosphatase isoform 5
NM_001287342.1	NP_001274271.1	14 kDa phosphohistidine phosphatase isoform 4
NM_001287343.2	NP_001274272.1	14 kDa phosphohistidine phosphatase isoform 5

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PHPT1	NP_001264621.1
Canis lupus familiaris (dog)	PHPT1	XP_005625137.1
Mus musculus (house mouse)	Phpt1	NP_083569.1
Drosophila melanogaster (fruit fly)	janA	NP_788763.1
Xenopus tropicalis (tropical clawed frog)	phpt1	XP_002940786.1
Caenorhabditis elegans (roundworm)	phip-1	NP_492382.2
Danio rerio (zebrafish)	phpt1	NP_001003566.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002078	XP_320969.4
Homo sapiens (human)	PHPT1	NP_001274271.1
Bos taurus (cattle)	PHPT1	NP_001069984.1
Rattus norvegicus (Norway rat)	Phpt1	NP_001100028.2

Gene Ontology
Bibliography

Related articles in PubMed

PHP14 regulates hepatic stellate cells migration in liver fibrosis via mediating TGF-?1 signaling to PI3K?/AKT/Rac1 pathway.
Xu A, Li Y, Zhao W, Hou F, Li X, Sun L, Chen W, Yang A, Wu S, Zhang B, Yao J, Wang H, Huang J
Journal of molecular medicine (Berlin, Germany)96(2)119-133(2018 Feb)

Structural and activity characterization of human PHPT1 after oxidative modification.
Martin DR, Dutta P, Mahajan S, Varma S, Stevens SM Jr
Scientific reports623658(2016 Apr)

Nuclear expression and clinical significance of phosphohistidine phosphatase 1 in clear-cell renal cell carcinoma.
Shen H, Yang P, Liu Q, Tian Y
The Journal of international medical research43(6)747-57(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

H2O2 exposure induces selective oxidation of hPHPT1 at Met95, a residue within the substrate binding region. Molecular dynamics simulations suggest that if Met95 oxidation alters hPHPT1 activity, then it will do so by altering the stability of an intermediate state. Mass spectrometry showed that H2O2-induced oxidation does not impact hPHPT1 function negatively.
Title: Structural and activity characterization of human PHPT1 after oxidative modification.

PHPT1 was expressed in the epithelium of proximal tubuli and nuclei of clear-cell renal cell carcinoma tissue samples.
Title: Nuclear expression and clinical significance of phosphohistidine phosphatase 1 in clear-cell renal cell carcinoma.

PHPT1 can dephosphorylate phospholysine in chemically phosphorylated histone H1 and polylysine
Title: Phosphohistidine phosphatase 1 (PHPT1) also dephosphorylates phospholysine of chemically phosphorylated histone H1 and polylysine.

The specific degradation of the PHPT1 splice variant indicates that the quality control and the self-guarding of the cellular system works at two levels, first at the RNA level and proteasome level.
Title: A splice variant of the human phosphohistidine phosphatase 1 (PHPT1) is degraded by the proteasome.

the histidine phosphatase, protein histidine phosphatase 1, inhibits NDPK-B-activated TRPV5 in inside/out patch experiments.
Title: Regulation of the epithelial Ca?? channel TRPV5 by reversible histidine phosphorylation mediated by NDPK-B and PHPT1.

The following PHPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01039	NM_014172.6 Latest version!	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00962	NM_001287343.2 Latest version!	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu01004	NM_001135861.3 Latest version!	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu01014	NM_001287342.2 Latest version!	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu01039	NM_014172.5	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01004	NM_001135861.2	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu01014	NM_001287342.1	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00962	NM_001287343.1	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHPT1 ( NM_001287342.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274271.1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01014
Clone ID Related Accession (Same CDS sequence)	NM_001287342.2 , NM_001287342.1
Accession Version	NM_001287342.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG681446.1, AF131857.1, CN334800.1, BP375594.1 and CN334799.1. Transcript Variant: This variant (4) encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP375594.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG GTGAGGCGGA CATCTACGAC 
AAAGTGTCGG GCGACATGCA GAAGCAAGGC TGCGACTGTG AGTGTCTGGG CGGCGGGCGC 
ATCTCCCACC AGAGTCAGGA CAAGAAGATT CACGTGTACG GCTATTCCAT GGCCTATGGT 
CCTGCCCAGC ACGCCATTTC AACTGAGAAA ATCAAAGCCA AGTACCCCGA CTACGAGGTC 
ACCTGGGCTA ACGACGGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274271.1
CDS	616..999
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHGEADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY

Target ORF information:

RefSeq Version	NM_001287342.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287342.1

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG GTGAGGCGGA CATCTACGAC 
AAAGTGTCGG GCGACATGCA GAAGCAAGGC TGCGACTGTG AGTGTCTGGG CGGCGGGCGC 
ATCTCCCACC AGAGTCAGGA CAAGAAGATT CACGTGTACG GCTATTCCAT GGCCTATGGT 
CCTGCCCAGC ACGCCATTTC AACTGAGAAA ATCAAAGCCA AGTACCCCGA CTACGAGGTC 
ACCTGGGCTA ACGACGGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_014172.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_054891.2 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01039
Clone ID Related Accession (Same CDS sequence)	NM_014172.5 , NM_014172.6
Accession Version	NM_014172.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG681446.1, AF131857.1, BC024648.1 and CN334799.1. On Dec 12, 2013 this sequence version replaced NM_014172.4. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 3, which is shorter than isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN334799.1, SRR1163657.323710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC 
CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG 
GCTAACGACG GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054891.2
CDS	616..993
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY

Target ORF information:

RefSeq Version	NM_014172.5
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014172.5

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC 
CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG 
GCTAACGACG GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_014172.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_054891.2 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01039
Clone ID Related Accession (Same CDS sequence)	NM_014172.5 , NM_014172.6
Accession Version	NM_014172.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355987.31. On Jul 19, 2019 this sequence version replaced NM_014172.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD388659.1, BP296211.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC 
CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG 
GCTAACGACG GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054891.2
CDS	23..400
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY

Target ORF information:

RefSeq Version	NM_014172.6
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014172.6

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC 
CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG 
GCTAACGACG GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_001287343.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274272.1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu00962
Clone ID Related Accession (Same CDS sequence)	NM_001287343.1 , NM_001287343.2
Accession Version	NM_001287343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG681446.1, AF131857.1, BE962935.2, CD693554.1 and CN334799.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 4. This results in a frameshift and an early stop codon. It encodes isoform 5, which is shorter and has a distinct C-terminus, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE962935.2, SRR1163655.557142.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGCAGAT GAGACCCACG 
TGCGTCCCTC TGGGCGCCTC AGGCCCCAGG ATCCACCATC AAGGCCTATG GTCCTGCCCA 
GCACGCCATT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274272.1
CDS	616..993
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMQMRPTCVPLGASGPRIHHQGLWSCPARHFN

Target ORF information:

RefSeq Version	NM_001287343.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287343.1

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGCAGAT GAGACCCACG 
TGCGTCCCTC TGGGCGCCTC AGGCCCCAGG ATCCACCATC AAGGCCTATG GTCCTGCCCA 
GCACGCCATT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_001135861.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129333.1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01004
Clone ID Related Accession (Same CDS sequence)	NM_001135861.2 , NM_001135861.3
Accession Version	NM_001135861.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG681446.1, AF131857.1, CD242442.1 and CN334799.1. On Dec 12, 2013 this sequence version replaced NM_001135861.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 4. This results in a frameshift and an early stop codon. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD242442.1, BM975420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGATGAG ACCCACGTGC 
GTCCCTCTGG GCGCCTCAGG CCCCAGGATC CACCATCAAG GCCTATGGTC CTGCCCAGCA 
CGCCATTTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129333.1
CDS	616..990
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMMRPTCVPLGASGPRIHHQGLWSCPARHFN

Target ORF information:

RefSeq Version	NM_001135861.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135861.2

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGATGAG ACCCACGTGC 
GTCCCTCTGG GCGCCTCAGG CCCCAGGATC CACCATCAAG GCCTATGGTC CTGCCCAGCA 
CGCCATTTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_001287343.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274272.1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 5, mRNA.

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CloneID	OHu00962
Clone ID Related Accession (Same CDS sequence)	NM_001287343.1 , NM_001287343.2
Accession Version	NM_001287343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355987.31. On Jul 19, 2019 this sequence version replaced NM_001287343.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE962935.2, SRR5189655.158715.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGCAGAT GAGACCCACG 
TGCGTCCCTC TGGGCGCCTC AGGCCCCAGG ATCCACCATC AAGGCCTATG GTCCTGCCCA 
GCACGCCATT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274272.1
CDS	23..400
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMQMRPTCVPLGASGPRIHHQGLWSCPARHFN

Target ORF information:

RefSeq Version	NM_001287343.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287343.2

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGCAGAT GAGACCCACG 
TGCGTCCCTC TGGGCGCCTC AGGCCCCAGG ATCCACCATC AAGGCCTATG GTCCTGCCCA 
GCACGCCATT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_001135861.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129333.1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 2, mRNA.

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CloneID	OHu01004
Clone ID Related Accession (Same CDS sequence)	NM_001135861.2 , NM_001135861.3
Accession Version	NM_001135861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355987.31. On Jul 19, 2019 this sequence version replaced NM_001135861.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM975420.1, CD242442.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGATGAG ACCCACGTGC 
GTCCCTCTGG GCGCCTCAGG CCCCAGGATC CACCATCAAG GCCTATGGTC CTGCCCAGCA 
CGCCATTTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129333.1
CDS	23..397
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMMRPTCVPLGASGPRIHHQGLWSCPARHFN

Target ORF information:

RefSeq Version	NM_001135861.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135861.3

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG 
TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC 
CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGATGAG ACCCACGTGC 
GTCCCTCTGG GCGCCTCAGG CCCCAGGATC CACCATCAAG GCCTATGGTC CTGCCCAGCA 
CGCCATTTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHPT1 ( NM_001287342.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274271.1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01014
Clone ID Related Accession (Same CDS sequence)	NM_001287342.2 , NM_001287342.1
Accession Version	NM_001287342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	14 kDa phosphohistidine phosphatase isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355987.31. On Jul 19, 2019 this sequence version replaced NM_001287342.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP375594.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG GTGAGGCGGA CATCTACGAC 
AAAGTGTCGG GCGACATGCA GAAGCAAGGC TGCGACTGTG AGTGTCTGGG CGGCGGGCGC 
ATCTCCCACC AGAGTCAGGA CAAGAAGATT CACGTGTACG GCTATTCCAT GGCCTATGGT 
CCTGCCCAGC ACGCCATTTC AACTGAGAAA ATCAAAGCCA AGTACCCCGA CTACGAGGTC 
ACCTGGGCTA ACGACGGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274271.1
CDS	23..406
Translation	MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHGEADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY

Target ORF information:

RefSeq Version	NM_001287342.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287342.2

ORF Insert Sequence:

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC 
AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC 
AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG GTGAGGCGGA CATCTACGAC 
AAAGTGTCGG GCGACATGCA GAAGCAAGGC TGCGACTGTG AGTGTCTGGG CGGCGGGCGC 
ATCTCCCACC AGAGTCAGGA CAAGAAGATT CACGTGTACG GCTATTCCAT GGCCTATGGT 
CCTGCCCAGC ACGCCATTTC AACTGAGAAA ATCAAAGCCA AGTACCCCGA CTACGAGGTC 
ACCTGGGCTA ACGACGGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PHP14 regulates hepatic stellate cells migration in liver fibrosis via mediating TGF-?1 signaling to PI3K?/AKT/Rac1 pathway.
Journal of molecular medicine (Berlin, Germany)96(2)119-133(2018 Feb)
Xu A,Li Y,Zhao W,Hou F,Li X,Sun L,Chen W,Yang A,Wu S,Zhang B,Yao J,Wang H,Huang J

Structural and activity characterization of human PHPT1 after oxidative modification.
Scientific reports623658(2016 Apr)
Martin DR,Dutta P,Mahajan S,Varma S,Stevens SM Jr

Nuclear expression and clinical significance of phosphohistidine phosphatase 1 in clear-cell renal cell carcinoma.
The Journal of international medical research43(6)747-57(2015 Dec)
Shen H,Yang P,Liu Q,Tian Y

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PHPT1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム