BCDIN3D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCDIN3D
Entrez Gene ID	144233
Full Name	BCDIN3 domain containing RNA methyltransferase
General protein information	Preferred Names BCDIN3 domain containing RNA methyltransferase Names pre-miRNA 5'-monophosphate methyltransferase BCDIN3 domain containing RNA methyltransfease BCDIN3 domain-containing protein probable methyltransferase BCDIN3D
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.12
Summary	This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. [provided by RefSeq, Apr 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_181708.3	NP_859059.1	RNA 5'-monophosphate methyltransferase
NM_181708.2	NP_859059.1	pre-miRNA 5'-monophosphate methyltransferase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Bcdin3d	NP_083512.2
Rattus norvegicus (Norway rat)	Bcdin3d	NP_001102221.1
Danio rerio (zebrafish)	LOC568375	XP_696793.3
Xenopus tropicalis (tropical clawed frog)	bcdin3d	XP_002935777.1
Homo sapiens (human)	BCDIN3D	NP_859059.1
Pan troglodytes (chimpanzee)	BCDIN3D	XP_001157619.1
Canis lupus familiaris (dog)	BCDIN3D	XP_003433554.1
Drosophila melanogaster (fruit fly)	CG11342	NP_647896.1
	BCDIN3D	XP_001110415.1
Bos taurus (cattle)	BCDIN3D	NP_001068684.1
Gallus gallus (chicken)	BCDIN3D	XP_428854.4

Gene Ontology
Bibliography

Related articles in PubMed

Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA.
Martinez A, Yamashita S, Nagaike T, Sakaguchi Y, Suzuki T, Tomita K
Nucleic acids research45(9)5423-5436(2017 May)

A Suite of Biochemical Assays for Screening RNA Methyltransferase BCDIN3D.
Blazer LL, Li F, Kennedy S, Zheng YG, Arrowsmith CH, Vedadi M
SLAS discovery : advancing life sciences R & D22(1)32-39(2017 Jan)

Elevated expression of RNA methyltransferase BCDIN3D predicts poor prognosis in breast cancer.
Yao L, Chi Y, Hu X, Li S, Qiao F, Wu J, Shao ZM
Oncotarget7(33)53895-53902(2016 Aug)

RNA methyltransferases involved in 5' cap biosynthesis.
Byszewska M, ?mieta?ski M, Purta E, Bujnicki JM
RNA biology11(12)1597-607(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BCDIN3D acts as a cytoplasmic tRNAHis-specific 5-methylphosphate capping enzyme.
Title: Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA.

BCDIN3D depletion leads to lower pre-miR-145 and concomitantly increased mature miR-145 levels in breast cancer cells, which suppresses their tumorigenic phenotypes.
Title: Human RNA methyltransferase BCDIN3D regulates microRNA processing.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic susceptibility to obesity and related traits in childhood and adolescence: influence of loci identified by genome-wide association studies.

Observational study of gene-disease association. (HuGE Navigator)
Title: Analyses of shared genetic factors between asthma and obesity in children.

Observational study of gene-disease association. (HuGE Navigator)
Title: Obesity and diabetes genes are associated with being born small for gestational age: results from the Auckland Birthweight Collaborative study.

The following BCDIN3D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCDIN3D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32069	NM_181708.3 Latest version!	Homo sapiens BCDIN3 domain containing RNA methyltransferase (BCDIN3D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu32069	NM_181708.2	Homo sapiens BCDIN3 domain containing RNA methyltransferase (BCDIN3D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCDIN3D ( NM_181708.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_859059.1 Homo sapiens BCDIN3 domain containing RNA methyltransferase (BCDIN3D), mRNA.

Price & Availability↑

CloneID	OHu32069
Clone ID Related Accession (Same CDS sequence)	NM_181708.3 , NM_181708.2
Accession Version	NM_181708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	RNA 5'-monophosphate methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD690663.1, BC053560.1, AC131157.4 and BU622810.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_181708.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.55522.1, SRR1803615.264520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333924.6/ ENSP00000335201.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGTGC CCACGGAACT GGATGGAGGG AGTGTTAAGG AGACCGCAGC GGAAGAGGAA 
TCGCGAGTTC TGGCACCTGG CGCCGCCCCG TTCGGAAATT TTCCTCATTA TTCTCGCTTC 
CACCCTCCGG AGCAACGGCT CCGCCTCCTG CCCCCGGAGC TGCTTCGACA GCTCTTTCCT 
GAGAGTCCCG AGAACGGGCC GATTCTGGGG CTCGACGTGG GGTGTAACTC CGGGGATCTG 
AGTGTGGCTC TATACAAACA CTTCCTCTCC CTACCTGACG GGGAAACCTG CTCAGATGCC 
TCAAGAGAAT TCCGTCTCCT CTGCTGCGAC ATAGATCCAG TCCTGGTGAA GCGAGCCGAA 
AAAGAATGTC CTTTTCCTGA TGCCTTGACT TTTATCACCC TGGACTTCAT GAATCAAAGG 
ACCCGGAAGG TTCTCTTGAG CTCTTTCTTA AGCCAATTTG GACGTTCAGT TTTTGACATT 
GGCTTCTGCA TGTCAATAAC CATGTGGATT CATCTGAATC ATGGAGACCA TGGCCTATGG 
GAGTTCCTGG CCCATCTTTC CTCCCTCTGC CACTACCTCC TTGTGGAGCC CCAACCCTGG 
AAGTGTTACC GGGCAGCTGC AAGGCGTCTC CGAAAGCTGG GACTCCATGA TTTTGACCAC 
TTCCACTCCC TTGCCATCCG AGGTGACATG CCCAATCAGA TTGTGCAGAT CTTGACCCAG 
GATCATGGCA TGGAATTAAT ATGTTGCTTT GGCAACACCA GTTGGGACAG AAGCCTTCTG 
CTCTTCAGGG CAAAACAAAC CATAGAGACT CATCCAATCC CTGAATCACT GATAGAAAAA 
GGGAAAGAAA AGAACAGATT AAGTTTCCAG AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_859059.1
CDS	20..898
Translation	MAVPTELDGGSVKETAAEEESRVLAPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPESPENGPILGLDVGCNSGDLSVALYKHFLSLPDGETCSDASREFRLLCCDIDPVLVKRAEKECPFPDALTFITLDFMNQRTRKVLLSSFLSQFGRSVFDIGFCMSITMWIHLNHGDHGLWEFLAHLSSLCHYLLVEPQPWKCYRAAARRLRKLGLHDFDHFHSLAIRGDMPNQIVQILTQDHGMELICCFGNTSWDRSLLLFRAKQTIETHPIPESLIEKGKEKNRLSFQKQ

Target ORF information:

RefSeq Version	NM_181708.3
Organism	Homo sapiens(human)
Definition	Homo sapiens BCDIN3 domain containing RNA methyltransferase (BCDIN3D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181708.3

ORF Insert Sequence:

ATGGCGGTGC CCACGGAACT GGATGGAGGG AGTGTTAAGG AGACCGCAGC GGAAGAGGAA 
TCGCGAGTTC TGGCACCTGG CGCCGCCCCG TTCGGAAATT TTCCTCATTA TTCTCGCTTC 
CACCCTCCGG AGCAACGGCT CCGCCTCCTG CCCCCGGAGC TGCTTCGACA GCTCTTTCCT 
GAGAGTCCCG AGAACGGGCC GATTCTGGGG CTCGACGTGG GGTGTAACTC CGGGGATCTG 
AGTGTGGCTC TATACAAACA CTTCCTCTCC CTACCTGACG GGGAAACCTG CTCAGATGCC 
TCAAGAGAAT TCCGTCTCCT CTGCTGCGAC ATAGATCCAG TCCTGGTGAA GCGAGCCGAA 
AAAGAATGTC CTTTTCCTGA TGCCTTGACT TTTATCACCC TGGACTTCAT GAATCAAAGG 
ACCCGGAAGG TTCTCTTGAG CTCTTTCTTA AGCCAATTTG GACGTTCAGT TTTTGACATT 
GGCTTCTGCA TGTCAATAAC CATGTGGATT CATCTGAATC ATGGAGACCA TGGCCTATGG 
GAGTTCCTGG CCCATCTTTC CTCCCTCTGC CACTACCTCC TTGTGGAGCC CCAACCCTGG 
AAGTGTTACC GGGCAGCTGC AAGGCGTCTC CGAAAGCTGG GACTCCATGA TTTTGACCAC 
TTCCACTCCC TTGCCATCCG AGGTGACATG CCCAATCAGA TTGTGCAGAT CTTGACCCAG 
GATCATGGCA TGGAATTAAT ATGTTGCTTT GGCAACACCA GTTGGGACAG AAGCCTTCTG 
CTCTTCAGGG CAAAACAAAC CATAGAGACT CATCCAATCC CTGAATCACT GATAGAAAAA 
GGGAAAGAAA AGAACAGATT AAGTTTCCAG AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCDIN3D ( NM_181708.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_859059.1 Homo sapiens BCDIN3 domain containing RNA methyltransferase (BCDIN3D), mRNA.

Price & Availability↑

CloneID	OHu32069
Clone ID Related Accession (Same CDS sequence)	NM_181708.3 , NM_181708.2
Accession Version	NM_181708.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-24
Organism	Homo sapiens(human)
Product	pre-miRNA 5'-monophosphate methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD690663.1, BC053560.1, AC131157.4 and BU622810.1. This sequence is a reference standard in the RefSeqGene project. On Apr 25, 2009 this sequence version replaced NM_181708.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.264520.1, SRR1803612.187727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGC CCACGGAACT GGATGGAGGG AGTGTTAAGG AGACCGCAGC GGAAGAGGAA 
TCGCGAGTTC TGGCACCTGG CGCCGCCCCG TTCGGAAATT TTCCTCATTA TTCTCGCTTC 
CACCCTCCGG AGCAACGGCT CCGCCTCCTG CCCCCGGAGC TGCTTCGACA GCTCTTTCCT 
GAGAGTCCCG AGAACGGGCC GATTCTGGGG CTCGACGTGG GGTGTAACTC CGGGGATCTG 
AGTGTGGCTC TATACAAACA CTTCCTCTCC CTACCTGACG GGGAAACCTG CTCAGATGCC 
TCAAGAGAAT TCCGTCTCCT CTGCTGCGAC ATAGATCCAG TCCTGGTGAA GCGAGCCGAA 
AAAGAATGTC CTTTTCCTGA TGCCTTGACT TTTATCACCC TGGACTTCAT GAATCAAAGG 
ACCCGGAAGG TTCTCTTGAG CTCTTTCTTA AGCCAATTTG GACGTTCAGT TTTTGACATT 
GGCTTCTGCA TGTCAATAAC CATGTGGATT CATCTGAATC ATGGAGACCA TGGCCTATGG 
GAGTTCCTGG CCCATCTTTC CTCCCTCTGC CACTACCTCC TTGTGGAGCC CCAACCCTGG 
AAGTGTTACC GGGCAGCTGC AAGGCGTCTC CGAAAGCTGG GACTCCATGA TTTTGACCAC 
TTCCACTCCC TTGCCATCCG AGGTGACATG CCCAATCAGA TTGTGCAGAT CTTGACCCAG 
GATCATGGCA TGGAATTAAT ATGTTGCTTT GGCAACACCA GTTGGGACAG AAGCCTTCTG 
CTCTTCAGGG CAAAACAAAC CATAGAGACT CATCCAATCC CTGAATCACT GATAGAAAAA 
GGGAAAGAAA AGAACAGATT AAGTTTCCAG AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_859059.1
CDS	43..921
Translation	MAVPTELDGGSVKETAAEEESRVLAPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPESPENGPILGLDVGCNSGDLSVALYKHFLSLPDGETCSDASREFRLLCCDIDPVLVKRAEKECPFPDALTFITLDFMNQRTRKVLLSSFLSQFGRSVFDIGFCMSITMWIHLNHGDHGLWEFLAHLSSLCHYLLVEPQPWKCYRAAARRLRKLGLHDFDHFHSLAIRGDMPNQIVQILTQDHGMELICCFGNTSWDRSLLLFRAKQTIETHPIPESLIEKGKEKNRLSFQKQ

Target ORF information:

RefSeq Version	NM_181708.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BCDIN3 domain containing RNA methyltransferase (BCDIN3D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181708.2

ORF Insert Sequence:

ATGGCGGTGC CCACGGAACT GGATGGAGGG AGTGTTAAGG AGACCGCAGC GGAAGAGGAA 
TCGCGAGTTC TGGCACCTGG CGCCGCCCCG TTCGGAAATT TTCCTCATTA TTCTCGCTTC 
CACCCTCCGG AGCAACGGCT CCGCCTCCTG CCCCCGGAGC TGCTTCGACA GCTCTTTCCT 
GAGAGTCCCG AGAACGGGCC GATTCTGGGG CTCGACGTGG GGTGTAACTC CGGGGATCTG 
AGTGTGGCTC TATACAAACA CTTCCTCTCC CTACCTGACG GGGAAACCTG CTCAGATGCC 
TCAAGAGAAT TCCGTCTCCT CTGCTGCGAC ATAGATCCAG TCCTGGTGAA GCGAGCCGAA 
AAAGAATGTC CTTTTCCTGA TGCCTTGACT TTTATCACCC TGGACTTCAT GAATCAAAGG 
ACCCGGAAGG TTCTCTTGAG CTCTTTCTTA AGCCAATTTG GACGTTCAGT TTTTGACATT 
GGCTTCTGCA TGTCAATAAC CATGTGGATT CATCTGAATC ATGGAGACCA TGGCCTATGG 
GAGTTCCTGG CCCATCTTTC CTCCCTCTGC CACTACCTCC TTGTGGAGCC CCAACCCTGG 
AAGTGTTACC GGGCAGCTGC AAGGCGTCTC CGAAAGCTGG GACTCCATGA TTTTGACCAC 
TTCCACTCCC TTGCCATCCG AGGTGACATG CCCAATCAGA TTGTGCAGAT CTTGACCCAG 
GATCATGGCA TGGAATTAAT ATGTTGCTTT GGCAACACCA GTTGGGACAG AAGCCTTCTG 
CTCTTCAGGG CAAAACAAAC CATAGAGACT CATCCAATCC CTGAATCACT GATAGAAAAA 
GGGAAAGAAA AGAACAGATT AAGTTTCCAG AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA.
Nucleic acids research45(9)5423-5436(2017 May)
Martinez A,Yamashita S,Nagaike T,Sakaguchi Y,Suzuki T,Tomita K

A Suite of Biochemical Assays for Screening RNA Methyltransferase BCDIN3D.
SLAS discovery : advancing life sciences R & D22(1)32-39(2017 Jan)
Blazer LL,Li F,Kennedy S,Zheng YG,Arrowsmith CH,Vedadi M

Elevated expression of RNA methyltransferase BCDIN3D predicts poor prognosis in breast cancer.
Oncotarget7(33)53895-53902(2016 Aug)
Yao L,Chi Y,Hu X,Li S,Qiao F,Wu J,Shao ZM

RNA methyltransferases involved in 5' cap biosynthesis.
RNA biology11(12)1597-607(2014)
Byszewska M,?mieta?ski M,Purta E,Bujnicki JM

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BCDIN3D cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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