mfhas1 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	mfhas1
Entrez Gene ID	102298828
Full Name	malignant fibrous histiocytoma amplified sequence 1
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005945025.2	XP_005945087.1	malignant fibrous histiocytoma-amplified sequence 1
XM_005945024.2	XP_005945086.1	malignant fibrous histiocytoma-amplified sequence 1

Pathways from BioSystems
Gene Ontology

The following mfhas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mfhas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa29707	XM_005945024.2 Latest version!	Haplochromis burtoni malignant fibrous histiocytoma amplified sequence 1 (mfhas1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHa29707	XM_005945025.2 Latest version!	Haplochromis burtoni malignant fibrous histiocytoma amplified sequence 1 (mfhas1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mfhas1 ( XM_005945025.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005945087.1 Haplochromis burtoni malignant fibrous histiocytoma amplified sequence 1 (mfhas1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHa29707
Clone ID Related Accession (Same CDS sequence)	XM_005945025.2 , XM_005945024.2
Accession Version	XM_005945025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	malignant fibrous histiocytoma-amplified sequence 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179837.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005945025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTC TCAATGAAAA CAAGGAGAAG GAGGAGGAGG AGGAGGGGTC CGGCTGCAGC 
GCCATGGAGG ACAAGGAGAA CAATCTGAAA ACGGCCAGGC TGTGGAGGGA TGCCGCCCTC 
CGCTCCAGGA AGCTGCGGAG CAACCTGAGG CAGCTCACTC TCTGCTCCAA AAACAACCAG 
ATCATCCTGC CAGAGGACAT AGCCGACATC GAGGCGCTCA ACCTGGGGAA CAACTCTCTG 
CAGGAGCTGC CGGACGGCCT GGAATCCTCC CTGAACAACC TGCGCGTCTT GGTCCTCCGC 
AGGAACAAGT TCAGCTCTGT CCCTAGGGTA GTGTTTGAGC TGGGGCGGCT GGTGGAGCTC 
GACATGAGCC ACAACTGTTT GAGGAGCCTT CCTGAGGGCG TGGGGCAGCT GAGAGGGCTG 
AAGAAGTTGT GCATCAGCCA CAACAAAATC CAGCACCTGC CAGCTCAGAT CGGAGCTCTG 
CAGTCTTTGG AGGAGCTCGA CATGAGCTTT AACGACCTGC ATGATTTCCC CAGGTCGTTC 
TCCGGCCTCG CCCACCTGCG AACTCTGGAT GCAGATCACA ACAAGCTGAA CCAGTTCCCC 
CCCGAGATCA TGGCCCTCAG TGAGCTGGAG GAGCTCGACT GCTCTGGGAA TAAGTTTGAG 
GTGTTACCGG TGGACGTTTG GAAGCTGCAG TCCATCAAAA TCCTGTGGCT CAGCAGCCTC 
CACATGTCCT CCTTACCCCA CACTTTCTGT CACCTGCAGC ACCTGGAGAG CCTGATGTTG 
GACGGGAACC ACCTCAGAGA GCTGCCCCCC TCCTTCGGCA GCCTGCAAAG ACTCAAAATG 
CTCAATCTGT CCTCAAATGA CTTTGAAAAC TTTCCCCAGG TTATTTTGAG CATAACGGGA 
TTAGAGGAGC TTTACCTGAG CAGGAATAGA CTGATTCATA TTCCAGAGGA AATCGGCCAG 
CTGGGGAAGC TGGTGAACCT GTGGCTGGAC AATAACAGCA TCACATATCT GCCTGACTCC 
ATCGTGGAGC TGGAGAATCT GGAGGAGCTC GTTTTACAGG GTAACCAAAT AGCCATTCTT 
CCAGATAATT TTGGAAAGCT TTCCAAAGTG AACATTTGGA AAGTGAAGGA TAACCCTCTG 
ATCCAGCCAC CGTACGAGGT GTGCATGAAG GGAATCCCAT ACATTGCAGT GTATCAGAAG 
GAGCTTGCAC ACTCTCAGTT TGCCGTGAAG CCACGGCTCA AACTGGTCCT GATGGGGACC 
CAAAACGCAG GGAAAACCCG GCTGAGGCAG AGCCTGGTGA GCACGCAGCA GGACGCCAGC 
AGTAACCAGG GAAACAAAGG AATTGAAGTC ACCAACTGGG TGGCAGACGC TGATCGCCAC 
CTCACGTTCC TCGTGTATGA CCTGTCAGGG AAGCAAAACT ACGACCTGAT CAAACCCTTC 
TTTCTCTCCC CCGGTGCTCT CTACATTCTC GTCATCAACC TCAAAACGTA CTCACCCAAG 
AACTTTTACA CCCATGTTGG GTATTTCCTC CACCTGCTCT GCGCCAAAGT GCCCCATGCT 
GTAGTCTGCT TGGTGGGCAC GCACACAGAC CTGTGTGCAG AGGTGGAGGT GGAGGAGAAG 
ACTCTGGACA TCCACAGGCA GATTGGCCTG CAGGAGAAGA GGGACATCCA GAGTTTAAGG 
GGGCTGGCAC TGCAGGTGGA CCAGGCTCTG GAGCAGGGCT ACAACGTCCG CACCTCCAGC 
CCTCACATCC TCTTTTACGG TGTCACTGAC AAAAACCTGA GGCGCCGAAA AGCCCAGCTG 
CAGTACATGC TGAACCACCG GCTGCAGATC CTCTCTCCTG TCCTGAGAGT TAGCTGCACA 
GGTGCTCAGA GGAACATCCC AAGGCTGAGG GAGAAGCTCA TGTCCGTTGC AGAACACAGG 
GAGATCTTCC CCAACCTCCA TCGTGTGCTG CCAAAGTCCT GGCAGATGCT GGAGGAGCTG 
CACTTTAAGC CCAAAGACCT GTGGCTCTCG TGGTGGGAAT CAGCCCGACT GGGCCTGCAG 
GCAGGGCTCA CTGAGGACCG GCTGCAGAGC GCTTTATCCT ACTTGCACGA GAGTGGGAAG 
CTGCTCTACT TTGAGGACAG CCTCACGCTA AGGGAATACG TTTTTCACAA TCTTCCACGA 
TTCATCGCAA TTCTCAATGT GTTCTTCCAG AGGGATGAGT CCACCCTGCT GGACCGGCTC 
CTCTCTGAAG GGGAGAGGGG GGACAAGGGG CGAGTGAGTC TGGTCATAGA GGACAAGAAG 
GGAGAGAACC TCAGGGTCAC CCATCTGCAG CACCACGTTG AGGGCTTCCT CCAGCATGGA 
CTCCTGCCCT CTAATGTCAT CCGCTTGCTC CTCAGACCGC TCATCCAGAC CCAGCAGGAC 
CTCCATCTCA TCATGGAGCT ACTGGAGAAA ATGGGGATCT GCTATTGCAT CAACAAACCC 
CGCAGCAAGC CTCTGAATGG CGCGACTGTG TGGTACAAGT TCCCCAGCTA CGTCAGCAGC 
GAGGAGGTCC AGGTGGATGC TTCAGCCGGC GGGAGCTCTC TGCCTCTGTG TAACTTCTTC 
TCTGTGGAGC AGCTGCACAT CCAGTACAGT TTCCCTTTCC TGTTTCCTCC TGGATTGTTC 
GCACGCTTCA GCGTGCAGAT CAACAGCCAC GTGGTGCAGC GTTCAGACAG CAAGCACCAG 
ATCTTCGCTT ATCGGGGTAA AGTTCCCGTG GTGATCGGCC ACCGGCCTGC CAAAGGAAAG 
CTGCAGGTGG AGACTCTTTC CATCGCCAGC CACGCCTCAC TGCCCAACAT CTGGACTGCC 
TGGCAGGCCA TCACTCCACT GGTGGAGGAG CTGAACATGC TGCTGCAGGA GTGGCCCGGT 
CTGCACTACT CTGTACATGT TCTCTGTTCC AAGTGCCTCA AGAGAGGGTC ATCCAACCCA 
CACGCCTTCC CAGGTGAGCT GCTGAGCCAG CCGCGGCCCG AAGGCCTCAC CGAGATCGTC 
TGTCCAAAGA ATGGCTCGGA GCGGGTCAAT GTGGCCCTCG TTTACCCCCC GACCCCCACC 
GTGCTCAGCC CCTGTCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005945087.1
CDS	405..3488
Translation	MKTLNENKEKEEEEEGSGCSAMEDKENNLKTARLWRDAALRSRKLRSNLRQLTLCSKNNQIILPEDIADIEALNLGNNSLQELPDGLESSLNNLRVLVLRRNKFSSVPRVVFELGRLVELDMSHNCLRSLPEGVGQLRGLKKLCISHNKIQHLPAQIGALQSLEELDMSFNDLHDFPRSFSGLAHLRTLDADHNKLNQFPPEIMALSELEELDCSGNKFEVLPVDVWKLQSIKILWLSSLHMSSLPHTFCHLQHLESLMLDGNHLRELPPSFGSLQRLKMLNLSSNDFENFPQVILSITGLEELYLSRNRLIHIPEEIGQLGKLVNLWLDNNSITYLPDSIVELENLEELVLQGNQIAILPDNFGKLSKVNIWKVKDNPLIQPPYEVCMKGIPYIAVYQKELAHSQFAVKPRLKLVLMGTQNAGKTRLRQSLVSTQQDASSNQGNKGIEVTNWVADADRHLTFLVYDLSGKQNYDLIKPFFLSPGALYILVINLKTYSPKNFYTHVGYFLHLLCAKVPHAVVCLVGTHTDLCAEVEVEEKTLDIHRQIGLQEKRDIQSLRGLALQVDQALEQGYNVRTSSPHILFYGVTDKNLRRRKAQLQYMLNHRLQILSPVLRVSCTGAQRNIPRLREKLMSVAEHREIFPNLHRVLPKSWQMLEELHFKPKDLWLSWWESARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSLTLREYVFHNLPRFIAILNVFFQRDESTLLDRLLSEGERGDKGRVSLVIEDKKGENLRVTHLQHHVEGFLQHGLLPSNVIRLLLRPLIQTQQDLHLIMELLEKMGICYCINKPRSKPLNGATVWYKFPSYVSSEEVQVDASAGGSSLPLCNFFSVEQLHIQYSFPFLFPPGLFARFSVQINSHVVQRSDSKHQIFAYRGKVPVVIGHRPAKGKLQVETLSIASHASLPNIWTAWQAITPLVEELNMLLQEWPGLHYSVHVLCSKCLKRGSSNPHAFPGELLSQPRPEGLTEIVCPKNGSERVNVALVYPPTPTVLSPCLK

Target ORF information:

RefSeq Version	XM_005945025.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni malignant fibrous histiocytoma amplified sequence 1 (mfhas1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005945025.2

ORF Insert Sequence:

ATGAAAACTC TCAATGAAAA CAAGGAGAAG GAGGAGGAGG AGGAGGGGTC CGGCTGCAGC 
GCCATGGAGG ACAAGGAGAA CAATCTGAAA ACGGCCAGGC TGTGGAGGGA TGCCGCCCTC 
CGCTCCAGGA AGCTGCGGAG CAACCTGAGG CAGCTCACTC TCTGCTCCAA AAACAACCAG 
ATCATCCTGC CAGAGGACAT AGCCGACATC GAGGCGCTCA ACCTGGGGAA CAACTCTCTG 
CAGGAGCTGC CGGACGGCCT GGAATCCTCC CTGAACAACC TGCGCGTCTT GGTCCTCCGC 
AGGAACAAGT TCAGCTCTGT CCCTAGGGTA GTGTTTGAGC TGGGGCGGCT GGTGGAGCTC 
GACATGAGCC ACAACTGTTT GAGGAGCCTT CCTGAGGGCG TGGGGCAGCT GAGAGGGCTG 
AAGAAGTTGT GCATCAGCCA CAACAAAATC CAGCACCTGC CAGCTCAGAT CGGAGCTCTG 
CAGTCTTTGG AGGAGCTCGA CATGAGCTTT AACGACCTGC ATGATTTCCC CAGGTCGTTC 
TCCGGCCTCG CCCACCTGCG AACTCTGGAT GCAGATCACA ACAAGCTGAA CCAGTTCCCC 
CCCGAGATCA TGGCCCTCAG TGAGCTGGAG GAGCTCGACT GCTCTGGGAA TAAGTTTGAG 
GTGTTACCGG TGGACGTTTG GAAGCTGCAG TCCATCAAAA TCCTGTGGCT CAGCAGCCTC 
CACATGTCCT CCTTACCCCA CACTTTCTGT CACCTGCAGC ACCTGGAGAG CCTGATGTTG 
GACGGGAACC ACCTCAGAGA GCTGCCCCCC TCCTTCGGCA GCCTGCAAAG ACTCAAAATG 
CTCAATCTGT CCTCAAATGA CTTTGAAAAC TTTCCCCAGG TTATTTTGAG CATAACGGGA 
TTAGAGGAGC TTTACCTGAG CAGGAATAGA CTGATTCATA TTCCAGAGGA AATCGGCCAG 
CTGGGGAAGC TGGTGAACCT GTGGCTGGAC AATAACAGCA TCACATATCT GCCTGACTCC 
ATCGTGGAGC TGGAGAATCT GGAGGAGCTC GTTTTACAGG GTAACCAAAT AGCCATTCTT 
CCAGATAATT TTGGAAAGCT TTCCAAAGTG AACATTTGGA AAGTGAAGGA TAACCCTCTG 
ATCCAGCCAC CGTACGAGGT GTGCATGAAG GGAATCCCAT ACATTGCAGT GTATCAGAAG 
GAGCTTGCAC ACTCTCAGTT TGCCGTGAAG CCACGGCTCA AACTGGTCCT GATGGGGACC 
CAAAACGCAG GGAAAACCCG GCTGAGGCAG AGCCTGGTGA GCACGCAGCA GGACGCCAGC 
AGTAACCAGG GAAACAAAGG AATTGAAGTC ACCAACTGGG TGGCAGACGC TGATCGCCAC 
CTCACGTTCC TCGTGTATGA CCTGTCAGGG AAGCAAAACT ACGACCTGAT CAAACCCTTC 
TTTCTCTCCC CCGGTGCTCT CTACATTCTC GTCATCAACC TCAAAACGTA CTCACCCAAG 
AACTTTTACA CCCATGTTGG GTATTTCCTC CACCTGCTCT GCGCCAAAGT GCCCCATGCT 
GTAGTCTGCT TGGTGGGCAC GCACACAGAC CTGTGTGCAG AGGTGGAGGT GGAGGAGAAG 
ACTCTGGACA TCCACAGGCA GATTGGCCTG CAGGAGAAGA GGGACATCCA GAGTTTAAGG 
GGGCTGGCAC TGCAGGTGGA CCAGGCTCTG GAGCAGGGCT ACAACGTCCG CACCTCCAGC 
CCTCACATCC TCTTTTACGG TGTCACTGAC AAAAACCTGA GGCGCCGAAA AGCCCAGCTG 
CAGTACATGC TGAACCACCG GCTGCAGATC CTCTCTCCTG TCCTGAGAGT TAGCTGCACA 
GGTGCTCAGA GGAACATCCC AAGGCTGAGG GAGAAGCTCA TGTCCGTTGC AGAACACAGG 
GAGATCTTCC CCAACCTCCA TCGTGTGCTG CCAAAGTCCT GGCAGATGCT GGAGGAGCTG 
CACTTTAAGC CCAAAGACCT GTGGCTCTCG TGGTGGGAAT CAGCCCGACT GGGCCTGCAG 
GCAGGGCTCA CTGAGGACCG GCTGCAGAGC GCTTTATCCT ACTTGCACGA GAGTGGGAAG 
CTGCTCTACT TTGAGGACAG CCTCACGCTA AGGGAATACG TTTTTCACAA TCTTCCACGA 
TTCATCGCAA TTCTCAATGT GTTCTTCCAG AGGGATGAGT CCACCCTGCT GGACCGGCTC 
CTCTCTGAAG GGGAGAGGGG GGACAAGGGG CGAGTGAGTC TGGTCATAGA GGACAAGAAG 
GGAGAGAACC TCAGGGTCAC CCATCTGCAG CACCACGTTG AGGGCTTCCT CCAGCATGGA 
CTCCTGCCCT CTAATGTCAT CCGCTTGCTC CTCAGACCGC TCATCCAGAC CCAGCAGGAC 
CTCCATCTCA TCATGGAGCT ACTGGAGAAA ATGGGGATCT GCTATTGCAT CAACAAACCC 
CGCAGCAAGC CTCTGAATGG CGCGACTGTG TGGTACAAGT TCCCCAGCTA CGTCAGCAGC 
GAGGAGGTCC AGGTGGATGC TTCAGCCGGC GGGAGCTCTC TGCCTCTGTG TAACTTCTTC 
TCTGTGGAGC AGCTGCACAT CCAGTACAGT TTCCCTTTCC TGTTTCCTCC TGGATTGTTC 
GCACGCTTCA GCGTGCAGAT CAACAGCCAC GTGGTGCAGC GTTCAGACAG CAAGCACCAG 
ATCTTCGCTT ATCGGGGTAA AGTTCCCGTG GTGATCGGCC ACCGGCCTGC CAAAGGAAAG 
CTGCAGGTGG AGACTCTTTC CATCGCCAGC CACGCCTCAC TGCCCAACAT CTGGACTGCC 
TGGCAGGCCA TCACTCCACT GGTGGAGGAG CTGAACATGC TGCTGCAGGA GTGGCCCGGT 
CTGCACTACT CTGTACATGT TCTCTGTTCC AAGTGCCTCA AGAGAGGGTC ATCCAACCCA 
CACGCCTTCC CAGGTGAGCT GCTGAGCCAG CCGCGGCCCG AAGGCCTCAC CGAGATCGTC 
TGTCCAAAGA ATGGCTCGGA GCGGGTCAAT GTGGCCCTCG TTTACCCCCC GACCCCCACC 
GTGCTCAGCC CCTGTCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfhas1 ( XM_005945024.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005945086.1 Haplochromis burtoni malignant fibrous histiocytoma amplified sequence 1 (mfhas1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHa29707
Clone ID Related Accession (Same CDS sequence)	XM_005945025.2 , XM_005945024.2
Accession Version	XM_005945024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	malignant fibrous histiocytoma-amplified sequence 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179837.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005945024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTC TCAATGAAAA CAAGGAGAAG GAGGAGGAGG AGGAGGGGTC CGGCTGCAGC 
GCCATGGAGG ACAAGGAGAA CAATCTGAAA ACGGCCAGGC TGTGGAGGGA TGCCGCCCTC 
CGCTCCAGGA AGCTGCGGAG CAACCTGAGG CAGCTCACTC TCTGCTCCAA AAACAACCAG 
ATCATCCTGC CAGAGGACAT AGCCGACATC GAGGCGCTCA ACCTGGGGAA CAACTCTCTG 
CAGGAGCTGC CGGACGGCCT GGAATCCTCC CTGAACAACC TGCGCGTCTT GGTCCTCCGC 
AGGAACAAGT TCAGCTCTGT CCCTAGGGTA GTGTTTGAGC TGGGGCGGCT GGTGGAGCTC 
GACATGAGCC ACAACTGTTT GAGGAGCCTT CCTGAGGGCG TGGGGCAGCT GAGAGGGCTG 
AAGAAGTTGT GCATCAGCCA CAACAAAATC CAGCACCTGC CAGCTCAGAT CGGAGCTCTG 
CAGTCTTTGG AGGAGCTCGA CATGAGCTTT AACGACCTGC ATGATTTCCC CAGGTCGTTC 
TCCGGCCTCG CCCACCTGCG AACTCTGGAT GCAGATCACA ACAAGCTGAA CCAGTTCCCC 
CCCGAGATCA TGGCCCTCAG TGAGCTGGAG GAGCTCGACT GCTCTGGGAA TAAGTTTGAG 
GTGTTACCGG TGGACGTTTG GAAGCTGCAG TCCATCAAAA TCCTGTGGCT CAGCAGCCTC 
CACATGTCCT CCTTACCCCA CACTTTCTGT CACCTGCAGC ACCTGGAGAG CCTGATGTTG 
GACGGGAACC ACCTCAGAGA GCTGCCCCCC TCCTTCGGCA GCCTGCAAAG ACTCAAAATG 
CTCAATCTGT CCTCAAATGA CTTTGAAAAC TTTCCCCAGG TTATTTTGAG CATAACGGGA 
TTAGAGGAGC TTTACCTGAG CAGGAATAGA CTGATTCATA TTCCAGAGGA AATCGGCCAG 
CTGGGGAAGC TGGTGAACCT GTGGCTGGAC AATAACAGCA TCACATATCT GCCTGACTCC 
ATCGTGGAGC TGGAGAATCT GGAGGAGCTC GTTTTACAGG GTAACCAAAT AGCCATTCTT 
CCAGATAATT TTGGAAAGCT TTCCAAAGTG AACATTTGGA AAGTGAAGGA TAACCCTCTG 
ATCCAGCCAC CGTACGAGGT GTGCATGAAG GGAATCCCAT ACATTGCAGT GTATCAGAAG 
GAGCTTGCAC ACTCTCAGTT TGCCGTGAAG CCACGGCTCA AACTGGTCCT GATGGGGACC 
CAAAACGCAG GGAAAACCCG GCTGAGGCAG AGCCTGGTGA GCACGCAGCA GGACGCCAGC 
AGTAACCAGG GAAACAAAGG AATTGAAGTC ACCAACTGGG TGGCAGACGC TGATCGCCAC 
CTCACGTTCC TCGTGTATGA CCTGTCAGGG AAGCAAAACT ACGACCTGAT CAAACCCTTC 
TTTCTCTCCC CCGGTGCTCT CTACATTCTC GTCATCAACC TCAAAACGTA CTCACCCAAG 
AACTTTTACA CCCATGTTGG GTATTTCCTC CACCTGCTCT GCGCCAAAGT GCCCCATGCT 
GTAGTCTGCT TGGTGGGCAC GCACACAGAC CTGTGTGCAG AGGTGGAGGT GGAGGAGAAG 
ACTCTGGACA TCCACAGGCA GATTGGCCTG CAGGAGAAGA GGGACATCCA GAGTTTAAGG 
GGGCTGGCAC TGCAGGTGGA CCAGGCTCTG GAGCAGGGCT ACAACGTCCG CACCTCCAGC 
CCTCACATCC TCTTTTACGG TGTCACTGAC AAAAACCTGA GGCGCCGAAA AGCCCAGCTG 
CAGTACATGC TGAACCACCG GCTGCAGATC CTCTCTCCTG TCCTGAGAGT TAGCTGCACA 
GGTGCTCAGA GGAACATCCC AAGGCTGAGG GAGAAGCTCA TGTCCGTTGC AGAACACAGG 
GAGATCTTCC CCAACCTCCA TCGTGTGCTG CCAAAGTCCT GGCAGATGCT GGAGGAGCTG 
CACTTTAAGC CCAAAGACCT GTGGCTCTCG TGGTGGGAAT CAGCCCGACT GGGCCTGCAG 
GCAGGGCTCA CTGAGGACCG GCTGCAGAGC GCTTTATCCT ACTTGCACGA GAGTGGGAAG 
CTGCTCTACT TTGAGGACAG CCTCACGCTA AGGGAATACG TTTTTCACAA TCTTCCACGA 
TTCATCGCAA TTCTCAATGT GTTCTTCCAG AGGGATGAGT CCACCCTGCT GGACCGGCTC 
CTCTCTGAAG GGGAGAGGGG GGACAAGGGG CGAGTGAGTC TGGTCATAGA GGACAAGAAG 
GGAGAGAACC TCAGGGTCAC CCATCTGCAG CACCACGTTG AGGGCTTCCT CCAGCATGGA 
CTCCTGCCCT CTAATGTCAT CCGCTTGCTC CTCAGACCGC TCATCCAGAC CCAGCAGGAC 
CTCCATCTCA TCATGGAGCT ACTGGAGAAA ATGGGGATCT GCTATTGCAT CAACAAACCC 
CGCAGCAAGC CTCTGAATGG CGCGACTGTG TGGTACAAGT TCCCCAGCTA CGTCAGCAGC 
GAGGAGGTCC AGGTGGATGC TTCAGCCGGC GGGAGCTCTC TGCCTCTGTG TAACTTCTTC 
TCTGTGGAGC AGCTGCACAT CCAGTACAGT TTCCCTTTCC TGTTTCCTCC TGGATTGTTC 
GCACGCTTCA GCGTGCAGAT CAACAGCCAC GTGGTGCAGC GTTCAGACAG CAAGCACCAG 
ATCTTCGCTT ATCGGGGTAA AGTTCCCGTG GTGATCGGCC ACCGGCCTGC CAAAGGAAAG 
CTGCAGGTGG AGACTCTTTC CATCGCCAGC CACGCCTCAC TGCCCAACAT CTGGACTGCC 
TGGCAGGCCA TCACTCCACT GGTGGAGGAG CTGAACATGC TGCTGCAGGA GTGGCCCGGT 
CTGCACTACT CTGTACATGT TCTCTGTTCC AAGTGCCTCA AGAGAGGGTC ATCCAACCCA 
CACGCCTTCC CAGGTGAGCT GCTGAGCCAG CCGCGGCCCG AAGGCCTCAC CGAGATCGTC 
TGTCCAAAGA ATGGCTCGGA GCGGGTCAAT GTGGCCCTCG TTTACCCCCC GACCCCCACC 
GTGCTCAGCC CCTGTCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005945086.1
CDS	405..3488
Translation	MKTLNENKEKEEEEEGSGCSAMEDKENNLKTARLWRDAALRSRKLRSNLRQLTLCSKNNQIILPEDIADIEALNLGNNSLQELPDGLESSLNNLRVLVLRRNKFSSVPRVVFELGRLVELDMSHNCLRSLPEGVGQLRGLKKLCISHNKIQHLPAQIGALQSLEELDMSFNDLHDFPRSFSGLAHLRTLDADHNKLNQFPPEIMALSELEELDCSGNKFEVLPVDVWKLQSIKILWLSSLHMSSLPHTFCHLQHLESLMLDGNHLRELPPSFGSLQRLKMLNLSSNDFENFPQVILSITGLEELYLSRNRLIHIPEEIGQLGKLVNLWLDNNSITYLPDSIVELENLEELVLQGNQIAILPDNFGKLSKVNIWKVKDNPLIQPPYEVCMKGIPYIAVYQKELAHSQFAVKPRLKLVLMGTQNAGKTRLRQSLVSTQQDASSNQGNKGIEVTNWVADADRHLTFLVYDLSGKQNYDLIKPFFLSPGALYILVINLKTYSPKNFYTHVGYFLHLLCAKVPHAVVCLVGTHTDLCAEVEVEEKTLDIHRQIGLQEKRDIQSLRGLALQVDQALEQGYNVRTSSPHILFYGVTDKNLRRRKAQLQYMLNHRLQILSPVLRVSCTGAQRNIPRLREKLMSVAEHREIFPNLHRVLPKSWQMLEELHFKPKDLWLSWWESARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSLTLREYVFHNLPRFIAILNVFFQRDESTLLDRLLSEGERGDKGRVSLVIEDKKGENLRVTHLQHHVEGFLQHGLLPSNVIRLLLRPLIQTQQDLHLIMELLEKMGICYCINKPRSKPLNGATVWYKFPSYVSSEEVQVDASAGGSSLPLCNFFSVEQLHIQYSFPFLFPPGLFARFSVQINSHVVQRSDSKHQIFAYRGKVPVVIGHRPAKGKLQVETLSIASHASLPNIWTAWQAITPLVEELNMLLQEWPGLHYSVHVLCSKCLKRGSSNPHAFPGELLSQPRPEGLTEIVCPKNGSERVNVALVYPPTPTVLSPCLK

Target ORF information:

RefSeq Version	XM_005945024.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni malignant fibrous histiocytoma amplified sequence 1 (mfhas1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005945024.2

ORF Insert Sequence:

ATGAAAACTC TCAATGAAAA CAAGGAGAAG GAGGAGGAGG AGGAGGGGTC CGGCTGCAGC 
GCCATGGAGG ACAAGGAGAA CAATCTGAAA ACGGCCAGGC TGTGGAGGGA TGCCGCCCTC 
CGCTCCAGGA AGCTGCGGAG CAACCTGAGG CAGCTCACTC TCTGCTCCAA AAACAACCAG 
ATCATCCTGC CAGAGGACAT AGCCGACATC GAGGCGCTCA ACCTGGGGAA CAACTCTCTG 
CAGGAGCTGC CGGACGGCCT GGAATCCTCC CTGAACAACC TGCGCGTCTT GGTCCTCCGC 
AGGAACAAGT TCAGCTCTGT CCCTAGGGTA GTGTTTGAGC TGGGGCGGCT GGTGGAGCTC 
GACATGAGCC ACAACTGTTT GAGGAGCCTT CCTGAGGGCG TGGGGCAGCT GAGAGGGCTG 
AAGAAGTTGT GCATCAGCCA CAACAAAATC CAGCACCTGC CAGCTCAGAT CGGAGCTCTG 
CAGTCTTTGG AGGAGCTCGA CATGAGCTTT AACGACCTGC ATGATTTCCC CAGGTCGTTC 
TCCGGCCTCG CCCACCTGCG AACTCTGGAT GCAGATCACA ACAAGCTGAA CCAGTTCCCC 
CCCGAGATCA TGGCCCTCAG TGAGCTGGAG GAGCTCGACT GCTCTGGGAA TAAGTTTGAG 
GTGTTACCGG TGGACGTTTG GAAGCTGCAG TCCATCAAAA TCCTGTGGCT CAGCAGCCTC 
CACATGTCCT CCTTACCCCA CACTTTCTGT CACCTGCAGC ACCTGGAGAG CCTGATGTTG 
GACGGGAACC ACCTCAGAGA GCTGCCCCCC TCCTTCGGCA GCCTGCAAAG ACTCAAAATG 
CTCAATCTGT CCTCAAATGA CTTTGAAAAC TTTCCCCAGG TTATTTTGAG CATAACGGGA 
TTAGAGGAGC TTTACCTGAG CAGGAATAGA CTGATTCATA TTCCAGAGGA AATCGGCCAG 
CTGGGGAAGC TGGTGAACCT GTGGCTGGAC AATAACAGCA TCACATATCT GCCTGACTCC 
ATCGTGGAGC TGGAGAATCT GGAGGAGCTC GTTTTACAGG GTAACCAAAT AGCCATTCTT 
CCAGATAATT TTGGAAAGCT TTCCAAAGTG AACATTTGGA AAGTGAAGGA TAACCCTCTG 
ATCCAGCCAC CGTACGAGGT GTGCATGAAG GGAATCCCAT ACATTGCAGT GTATCAGAAG 
GAGCTTGCAC ACTCTCAGTT TGCCGTGAAG CCACGGCTCA AACTGGTCCT GATGGGGACC 
CAAAACGCAG GGAAAACCCG GCTGAGGCAG AGCCTGGTGA GCACGCAGCA GGACGCCAGC 
AGTAACCAGG GAAACAAAGG AATTGAAGTC ACCAACTGGG TGGCAGACGC TGATCGCCAC 
CTCACGTTCC TCGTGTATGA CCTGTCAGGG AAGCAAAACT ACGACCTGAT CAAACCCTTC 
TTTCTCTCCC CCGGTGCTCT CTACATTCTC GTCATCAACC TCAAAACGTA CTCACCCAAG 
AACTTTTACA CCCATGTTGG GTATTTCCTC CACCTGCTCT GCGCCAAAGT GCCCCATGCT 
GTAGTCTGCT TGGTGGGCAC GCACACAGAC CTGTGTGCAG AGGTGGAGGT GGAGGAGAAG 
ACTCTGGACA TCCACAGGCA GATTGGCCTG CAGGAGAAGA GGGACATCCA GAGTTTAAGG 
GGGCTGGCAC TGCAGGTGGA CCAGGCTCTG GAGCAGGGCT ACAACGTCCG CACCTCCAGC 
CCTCACATCC TCTTTTACGG TGTCACTGAC AAAAACCTGA GGCGCCGAAA AGCCCAGCTG 
CAGTACATGC TGAACCACCG GCTGCAGATC CTCTCTCCTG TCCTGAGAGT TAGCTGCACA 
GGTGCTCAGA GGAACATCCC AAGGCTGAGG GAGAAGCTCA TGTCCGTTGC AGAACACAGG 
GAGATCTTCC CCAACCTCCA TCGTGTGCTG CCAAAGTCCT GGCAGATGCT GGAGGAGCTG 
CACTTTAAGC CCAAAGACCT GTGGCTCTCG TGGTGGGAAT CAGCCCGACT GGGCCTGCAG 
GCAGGGCTCA CTGAGGACCG GCTGCAGAGC GCTTTATCCT ACTTGCACGA GAGTGGGAAG 
CTGCTCTACT TTGAGGACAG CCTCACGCTA AGGGAATACG TTTTTCACAA TCTTCCACGA 
TTCATCGCAA TTCTCAATGT GTTCTTCCAG AGGGATGAGT CCACCCTGCT GGACCGGCTC 
CTCTCTGAAG GGGAGAGGGG GGACAAGGGG CGAGTGAGTC TGGTCATAGA GGACAAGAAG 
GGAGAGAACC TCAGGGTCAC CCATCTGCAG CACCACGTTG AGGGCTTCCT CCAGCATGGA 
CTCCTGCCCT CTAATGTCAT CCGCTTGCTC CTCAGACCGC TCATCCAGAC CCAGCAGGAC 
CTCCATCTCA TCATGGAGCT ACTGGAGAAA ATGGGGATCT GCTATTGCAT CAACAAACCC 
CGCAGCAAGC CTCTGAATGG CGCGACTGTG TGGTACAAGT TCCCCAGCTA CGTCAGCAGC 
GAGGAGGTCC AGGTGGATGC TTCAGCCGGC GGGAGCTCTC TGCCTCTGTG TAACTTCTTC 
TCTGTGGAGC AGCTGCACAT CCAGTACAGT TTCCCTTTCC TGTTTCCTCC TGGATTGTTC 
GCACGCTTCA GCGTGCAGAT CAACAGCCAC GTGGTGCAGC GTTCAGACAG CAAGCACCAG 
ATCTTCGCTT ATCGGGGTAA AGTTCCCGTG GTGATCGGCC ACCGGCCTGC CAAAGGAAAG 
CTGCAGGTGG AGACTCTTTC CATCGCCAGC CACGCCTCAC TGCCCAACAT CTGGACTGCC 
TGGCAGGCCA TCACTCCACT GGTGGAGGAG CTGAACATGC TGCTGCAGGA GTGGCCCGGT 
CTGCACTACT CTGTACATGT TCTCTGTTCC AAGTGCCTCA AGAGAGGGTC ATCCAACCCA 
CACGCCTTCC CAGGTGAGCT GCTGAGCCAG CCGCGGCCCG AAGGCCTCAC CGAGATCGTC 
TGTCCAAAGA ATGGCTCGGA GCGGGTCAAT GTGGCCCTCG TTTACCCCCC GACCCCCACC 
GTGCTCAGCC CCTGTCTCAA ATGA