TIMP3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMP3
Entrez Gene ID	396483
Full Name	TIMP metallopeptidase inhibitor 3
Synonyms	IMP-3,TIMP-3
General protein information	Preferred Names TIMP metallopeptidase inhibitor 3 Names metalloproteinase inhibitor 3 21 kDa protein of extracellular matrix TIMP metallopeptidase inhibitor 3 (Sorsby fundus dystrophy, pseudoinflammatory) tissue inhibitor of metalloproteinase 3 tissue inhibitor of metalloproteinases 3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205487.2	NP_990818.1	metalloproteinase inhibitor 3 precursor

Pathways from BioSystems

Homologies

	TIMP3	NP_001028128.1
Canis lupus familiaris (dog)	TIMP3	XP_538410.2
Rattus norvegicus (Norway rat)	Timp3	NP_037018.2
Gallus gallus (chicken)	TIMP3	NP_990818.1
Homo sapiens (human)	TIMP3	NP_000353.1
Pan troglodytes (chimpanzee)	TIMP3	XP_515097.2
Bos taurus (cattle)	TIMP3	NP_776898.2
Mus musculus (house mouse)	Timp3	NP_035725.1
Xenopus tropicalis (tropical clawed frog)	timp3	XP_002940366.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct regression and recrudescence.
Le?niak-Walentyn A, Hrabia A
Cell and tissue research366(2)443-454(2016 Nov)

Expression and localization of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct during maturation.
Le?niak-Walentyn A, Hrabia A
Cell and tissue research364(1)185-97(2016 Apr)

Mapping the ABCA4, IMPDH2 and TIMP3 genes in chicken.
Paton IR, Smith J, Burt DW
Animal genetics34(5)395-6(2003 Oct)

A new inhibitor of metalloproteinases from chicken: ChIMP-3. A third member of the TIMP family.
Pavloff N, Staskus PW, Kishnani NS, Hawkes SP
The Journal of biological chemistry267(24)17321-6(1992 Aug)

The 21-kDa protein is a transformation-sensitive metalloproteinase inhibitor of chicken fibroblasts.
Staskus PW, Masiarz FR, Pallanck LJ, Hawkes SP
The Journal of biological chemistry266(1)449-54(1991 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Real-time polymerase chain reaction and western blot revealed different expression of MMP-2, -7, -9 and TIMP-2 and -3 on mRNA and protein levels, respectively, as well as activity of MMP-2 and -9 by activity assay in the oviduct parts.
Title: Involvement of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct regression and recrudescence.

Protein level of MMP-9 was diminished, while MMP-7 and TIMP-3 were elevated in the oviduct of growing birds. MMP-2 and TIMP-2 protein levels remained constant, with a slight increase in MMP-2 concentration just before reaching maturity.
Title: Expression and localization of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct during maturation.

The following TIMP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00051	NM_205487.2 Latest version!	Gallus gallus TIMP metallopeptidase inhibitor 3 (TIMP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMP3 ( NM_205487.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990818.1 Gallus gallus TIMP metallopeptidase inhibitor 3 (TIMP3), mRNA.

Price & Availability↑

CloneID	OGa00051
Clone ID Related Accession (Same CDS sequence)	NM_205487.2
Accession Version	NM_205487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Gallus gallus(chicken)
Product	metalloproteinase inhibitor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M94531.1. On Aug 31, 2012 this sequence version replaced NM_205487.1. ##Evidence-Data-START## Transcript exon combination :: M94531.1, CR386139.1 [ECO:0000332] ##Evidence-Data-END##

ATGACGGCGT GGCTCGGCTT CCTCGCCGTG TTCCTGTGCA GCTGGAGCCT GCGGGACCTG 
GTGGCGGAGG CGTGCACTTG CGTCCCCATC CACCCGCAGG ACGCGTTCTG CAACTCCGAC 
ATCGTGATCC GTGCTAAAGT TGTGGGGAAG AAGCTCATGA AAGATGGACC ATTTGGAACA 
ATGCGATACA CAGTCAAGCA GATGAAGATG TACAGGGGCT TCCAGATAAT GCCACACGTT 
CAGTACATCT ACACAGAAGC CTCAGAGAGT CTTTGTGGTG TGAAACTGGA GGTCAACAAA 
TACCAGTATC TGATTACAGG CCGCGTGTAC GAAGGGAAGG TTTACACTGG CCTGTGCAAT 
TGGTATGAGA AATGGGACCG ACTGACTCTG TCCCAGCGTA AAGGACTGAA TCATCGTTAT 
CATCTGGGCT GTGGATGCAA GATTCGGCCC TGCTACTATT TGCCCTGCTT TGCCACCTCC 
AAGAATGAGT GCATTTGGAC AGACATGCTC TCCAACTTCG GCCACTCAGG ACACCAAGCG 
AAGCACTATG CCTGCATCCA GAGGGTGGAA GGTTACTGCA GCTGGTATAG AGGATGGGCG 
CCTCCAGATA AAACGATCAT CAATGCCACA GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990818.1
CDS	111..749
Translation	MTAWLGFLAVFLCSWSLRDLVAEACTCVPIHPQDAFCNSDIVIRAKVVGKKLMKDGPFGTMRYTVKQMKMYRGFQIMPHVQYIYTEASESLCGVKLEVNKYQYLITGRVYEGKVYTGLCNWYEKWDRLTLSQRKGLNHRYHLGCGCKIRPCYYLPCFATSKNECIWTDMLSNFGHSGHQAKHYACIQRVEGYCSWYRGWAPPDKTIINATDP

Target ORF information:

RefSeq Version	NM_205487.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TIMP metallopeptidase inhibitor 3 (TIMP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205487.2

ORF Insert Sequence:

ATGACGGCGT GGCTCGGCTT CCTCGCCGTG TTCCTGTGCA GCTGGAGCCT GCGGGACCTG 
GTGGCGGAGG CGTGCACTTG CGTCCCCATC CACCCGCAGG ACGCGTTCTG CAACTCCGAC 
ATCGTGATCC GTGCTAAAGT TGTGGGGAAG AAGCTCATGA AAGATGGACC ATTTGGAACA 
ATGCGATACA CAGTCAAGCA GATGAAGATG TACAGGGGCT TCCAGATAAT GCCACACGTT 
CAGTACATCT ACACAGAAGC CTCAGAGAGT CTTTGTGGTG TGAAACTGGA GGTCAACAAA 
TACCAGTATC TGATTACAGG CCGCGTGTAC GAAGGGAAGG TTTACACTGG CCTGTGCAAT 
TGGTATGAGA AATGGGACCG ACTGACTCTG TCCCAGCGTA AAGGACTGAA TCATCGTTAT 
CATCTGGGCT GTGGATGCAA GATTCGGCCC TGCTACTATT TGCCCTGCTT TGCCACCTCC 
AAGAATGAGT GCATTTGGAC AGACATGCTC TCCAACTTCG GCCACTCAGG ACACCAAGCG 
AAGCACTATG CCTGCATCCA GAGGGTGGAA GGTTACTGCA GCTGGTATAG AGGATGGGCG 
CCTCCAGATA AAACGATCAT CAATGCCACA GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct regression and recrudescence.
Cell and tissue research366(2)443-454(2016 Nov)
Le?niak-Walentyn A,Hrabia A

Expression and localization of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct during maturation.
Cell and tissue research364(1)185-97(2016 Apr)
Le?niak-Walentyn A,Hrabia A

Mapping the ABCA4, IMPDH2 and TIMP3 genes in chicken.
Animal genetics34(5)395-6(2003 Oct)
Paton IR,Smith J,Burt DW

A new inhibitor of metalloproteinases from chicken: ChIMP-3. A third member of the TIMP family.
The Journal of biological chemistry267(24)17321-6(1992 Aug)
Pavloff N,Staskus PW,Kishnani NS,Hawkes SP

The 21-kDa protein is a transformation-sensitive metalloproteinase inhibitor of chicken fibroblasts.
The Journal of biological chemistry266(1)449-54(1991 Jan)
Staskus PW,Masiarz FR,Pallanck LJ,Hawkes SP