MYL9 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYL9
Entrez Gene ID	396215
Full Name	myosin, light chain 9, regulatory
Synonyms	DTNB,G1,MLC-2,MLC2
General protein information	Preferred Names myosin, light chain 9, regulatory Names myosin regulatory light chain 2, smooth muscle major isoform isoform L20-A light polypeptide 9 myosin light chain 2 myosin regulatory light polypeptide 9 myosin, light polypeptide 9, regulatory smooth muscle major isoform
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015296240.1	XP_015151726.1	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
XM_015296241.2	XP_015151727.1	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
XM_015296241.1	XP_015151727.1	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
NM_205278.1	NP_990609.1	myosin regulatory light chain 2, smooth muscle major isoform
XM_015296240.2	XP_015151726.1	myosin regulatory light chain 2, smooth muscle major isoform isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYL9	NP_006088.2
Bos taurus (cattle)	MYL9	NP_001068702.1
Gallus gallus (chicken)	MYL9	NP_990609.1
Canis lupus familiaris (dog)	MYL9	NP_001239095.1
Danio rerio (zebrafish)	myl9b	NP_998377.1
Pan troglodytes (chimpanzee)	MYL9	XP_514618.3
Mus musculus (house mouse)	Myl9	NP_742116.1
Rattus norvegicus (Norway rat)	Myl9	NP_001094355.1
Xenopus tropicalis (tropical clawed frog)	myl9	NP_001096250.1

Gene Ontology
Bibliography

Related articles in PubMed

A ternary complex of transcription factors, Nish?d and NFATc4, and co-activator p300 bound to an intronic sequence, intronic regulatory element, is pivotal for the up-regulation of myosin light chain-2v gene in cardiac hypertrophy.
Mathew S, Mascareno E, Siddiqui MA
The Journal of biological chemistry279(39)41018-27(2004 Sep)

Two distinct factor-binding DNA elements in cardiac myosin light chain 2 gene are essential for repression of its expression in skeletal muscle. Isolation of a cDNA clone for repressor protein Nished.
Dhar M, Mascareno EM, Siddiqui MA
The Journal of biological chemistry272(29)18490-7(1997 Jul)

An insert in the motor domain determines the functional properties of expressed smooth muscle myosin isoforms.
Rovner AS, Freyzon Y, Trybus KM
Journal of muscle research and cell motility18(1)103-10(1997 Feb)

Phosphorylation site sequence of smooth muscle myosin light chain (Mr = 20 000).
Pearson RB, Jakes R, John M, Kendrick-Jones J, Kemp BE
FEBS letters168(1)108-12(1984 Mar)

Amino-acid sequence of the 20 000-molecular-weight light chain of chicken gizzard-muscle myosin.
Maita T, Chen JI, Matsuda G
European journal of biochemistry117(2)417-24(1981 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

myosin light chain-2v is upregulated in cardiac hypertrophy by the complex of Nished and NFATc4, and co-activator p300 bound to an intronic regulatory element
Title: A ternary complex of transcription factors, Nish?d and NFATc4, and co-activator p300 bound to an intronic sequence, intronic regulatory element, is pivotal for the up-regulation of myosin light chain-2v gene in cardiac hypertrophy.

Upstream regulatory sequences are identified and the authors show that CFDP1 binds the regulatory motif.
Title: Two distinct factor-binding DNA elements in cardiac myosin light chain 2 gene are essential for repression of its expression in skeletal muscle. Isolation of a cDNA clone for repressor protein Nished.

The following MYL9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00647	NM_205278.1 Latest version!	Gallus gallus myosin, light chain 9, regulatory (MYL9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa44653	XM_015296241.2 Latest version!	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGa44653	XM_015296240.2 Latest version!	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
OGa44653	XM_015296241.1	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGa44653	XM_015296240.1	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYL9 ( NM_205278.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990609.1 Gallus gallus myosin, light chain 9, regulatory (MYL9), mRNA.

Price & Availability↑

CloneID	OGa00647
Clone ID Related Accession (Same CDS sequence)	NM_205278.1
Accession Version	NM_205278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Gallus gallus(chicken)
Product	myosin regulatory light chain 2, smooth muscle major isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X06387.1. ##Evidence-Data-START## Transcript exon combination :: X06387.1, Y00983.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGGAAAA ACCCCACCGA CGAGTACCTG GAGGGGATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990609.1
CDS	68..586
Translation	MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_205278.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, light chain 9, regulatory (MYL9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205278.1

ORF Insert Sequence:

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGGAAAA ACCCCACCGA CGAGTACCTG GAGGGGATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL9 ( XM_015296240.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151726.1 Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa44653
Clone ID Related Accession (Same CDS sequence)	XM_015296240.1 , XM_015296241.2 , XM_015296241.1 , XM_015296240.2
Accession Version	XM_015296240.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151726.1
CDS	197..715
Translation	MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	XM_015296240.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296240.1

ORF Insert Sequence:

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL9 ( XM_015296241.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151727.1 Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa44653
Clone ID Related Accession (Same CDS sequence)	XM_015296240.1 , XM_015296241.2 , XM_015296241.1 , XM_015296240.2
Accession Version	XM_015296241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151727.1
CDS	87..605
Translation	MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	XM_015296241.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296241.2

ORF Insert Sequence:

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL9 ( XM_015296241.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151727.1 Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa44653
Clone ID Related Accession (Same CDS sequence)	XM_015296240.1 , XM_015296241.2 , XM_015296241.1 , XM_015296240.2
Accession Version	XM_015296241.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455990.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151727.1
CDS	87..605
Translation	MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	XM_015296241.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296241.1

ORF Insert Sequence:

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL9 ( XM_015296240.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151726.1 Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa44653
Clone ID Related Accession (Same CDS sequence)	XM_015296240.1 , XM_015296241.2 , XM_015296241.1 , XM_015296240.2
Accession Version	XM_015296240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin regulatory light chain 2, smooth muscle major isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151726.1
CDS	136..654
Translation	MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	XM_015296240.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, light chain 9, regulatory (MYL9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296240.2

ORF Insert Sequence:

ATGTCCAGCA AACGTGCCAA AGCCAAGACC ACCAAGAAGC GCCCGCAGCG CGCCACCTCC 
AATGTCTTCG CTATGTTCGA CCAGTCACAG ATCCAGGAGT TCAAGGAAGC TTTCAACATG 
ATCGACCAGA ACCGTGACGG GTTCATTGAC AAGGAGGATC TGCATGACAT GCTGGCTTCC 
ATGGGAAAGA ACCCCACCGA CGAGTACCTG GAGGGCATGA TGAGTGAGGC ACCGGGGCCC 
ATCAACTTCA CCATGTTCCT CACCATGTTT GGGGAGAAGC TGAATGGCAC CGACCCGGAG 
GATGTAATCC GCAATGCCTT TGCCTGCTTT GACGAGGAGG CGTCAGGGTT CATTCACGAG 
GACCATCTGC GTGAACTGCT GACCACCATG GGAGACAGGT TCACTGACGA GGAGGTGGAC 
GAGATGTACC GGGAGGCGCC CATCGACAAG AAGGGCAACT TCAACTATGT GGAGTTCACC 
CGCATCCTGA AGCACGGAGC TAAGGACAAG GACGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A ternary complex of transcription factors, Nish?d and NFATc4, and co-activator p300 bound to an intronic sequence, intronic regulatory element, is pivotal for the up-regulation of myosin light chain-2v gene in cardiac hypertrophy.
The Journal of biological chemistry279(39)41018-27(2004 Sep)
Mathew S,Mascareno E,Siddiqui MA

Two distinct factor-binding DNA elements in cardiac myosin light chain 2 gene are essential for repression of its expression in skeletal muscle. Isolation of a cDNA clone for repressor protein Nished.
The Journal of biological chemistry272(29)18490-7(1997 Jul)
Dhar M,Mascareno EM,Siddiqui MA

An insert in the motor domain determines the functional properties of expressed smooth muscle myosin isoforms.
Journal of muscle research and cell motility18(1)103-10(1997 Feb)
Rovner AS,Freyzon Y,Trybus KM

Phosphorylation site sequence of smooth muscle myosin light chain (Mr = 20 000).
FEBS letters168(1)108-12(1984 Mar)
Pearson RB,Jakes R,John M,Kendrick-Jones J,Kemp BE

Amino-acid sequence of the 20 000-molecular-weight light chain of chicken gizzard-muscle myosin.
European journal of biochemistry117(2)417-24(1981 Jul)
Maita T,Chen JI,Matsuda G