P2RX4 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	P2RX4
Entrez Gene ID	374166
Full Name	purinergic receptor P2X 4
General protein information	Preferred Names purinergic receptor P2X 4 Names P2X purinoceptor 4 purinergic receptor P2X, ligand-gated ion channel, 4
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204291.1	NP_989622.1	P2X purinoceptor 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	P2rx4	NP_113782.1
Homo sapiens (human)	P2RX4	NP_002551.2
Bos taurus (cattle)	P2RX4	NP_001029221.1
Gallus gallus (chicken)	P2RX4	NP_989622.1
Canis lupus familiaris (dog)	P2RX4	XP_003639955.1
Mus musculus (house mouse)	P2rx4	NP_035156.2
Danio rerio (zebrafish)	p2rx4a	NP_705939.1
Pan troglodytes (chimpanzee)	P2RX4	XP_509437.2
Xenopus tropicalis (tropical clawed frog)	p2rx4	XP_002937738.2

Gene Ontology
Bibliography

Related articles in PubMed

Ionotropic purinergic receptor P2X4 is involved in the regulation of chondrogenesis in chicken micromass cell cultures.
Fodor J, Matta C, Juh?sz T, Ol?h T, G?nczi M, Sz?jgy?rt? Z, Gergely P, Csernoch L, Z?k?ny R
Cell calcium45(5)421-30(2009 May)

Cloning, functional characterization and developmental expression of a P2X receptor from chick embryo.
Ruppelt A, Liang BT, Soto F
Progress in brain research12081-90(1999)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest a role of ionotropic purinergic signalling of P2X(4) in the generation of ATP-dependent Ca(2+) transients of differentiating chondroblasts.
Title: Ionotropic purinergic receptor P2X4 is involved in the regulation of chondrogenesis in chicken micromass cell cultures.

The following P2RX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P2RX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa25845	NM_204291.1 Latest version!	Gallus gallus purinergic receptor P2X 4 (P2RX4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P2RX4 ( NM_204291.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989622.1 Gallus gallus purinergic receptor P2X 4 (P2RX4), mRNA.

Price & Availability↑

CloneID	OGa25845
Clone ID Related Accession (Same CDS sequence)	NM_204291.1
Accession Version	NM_204291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	P2X purinoceptor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF218449.1. ##Evidence-Data-START## Transcript exon combination :: AF218449.1, AF020315.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTGCGT GCTGCGGGGC TGTGCGCGGC TTCCTCTTCG AGTACGACAC CCCCCGCATC 
GTGCTGATCC GCAGCCGCAA AGTCGGGCTG ATCAACCGCG CCGTGCAGCT CGCCATCCTC 
GCCTACGTGA TCGGCTGGGT CTTTCTGTGG GAGAAGGGCT ACCAGGAAAC AGATTCCGTG 
GTCAGTTCTG TAACCACGAA GGTGAAAGGG GTAACGATGA CAAACACATC CACCTTGGGA 
TCCAGGATCT GGGATGTAGC TGACTACGTC ATCCCTCCCC AGGAGAAGAA CGCTGTGTTT 
GTCATGACCA ACATGATATT CACACTGAAC CAGAGCCAAA GCCACTGCCC AGAGCTTCCA 
GATGATAACA CAGAGTGCAA CAACAACAGC AGCTGCGTTC CAGGATATGT CAGCACCCAC 
AGCAATGGTA TCCAGACCGG GGCGTGCATA CCGTACAACA GCAGCATCAA GACCTGTGAA 
GTCTTTGCAT GGTGCCCTGT GGAGGATGAC TATCACATAC CCAATCCGGC ATTCTTGCAA 
GGAGCTGAGA ACTTCACCAT TCTGGTAAAG AACAACATTT GGTATCCCAA GTTCAACTTC 
AGCAAGCGAA ACATCCTCCC CACTTTCAGT TCCTCTTATC TCAAGAACTG CATCTATGAT 
GCCCAGACAG ATCCCTTCTG CCCTATCTTT CGTCTGGGGA AAATAGTTGA AGCTGCAGGG 
CAGAACTTCC AGGAAATGGC TGTGGAGGGT GGGGTCATGG GGCTGCAGAT CAACTGGGAC 
TGCAACCTCG ACAGAGCTGC TTCCCACTGC GTGCCCAAGT ATTCCTTCCG GCGCCTGGAC 
AACAAGGACT CTGCCAACAC TATCTCACCT GGGTACAACT TCAGGTTTGC AAAATACTAC 
AAGGATAGTA GTGGCATTGA AACACGAACG CTTATCAAAG CTTATGGCAT CCGCCTTGAT 
ATCATAGTGT TTGGAAAGGC AGGAAAATTT GATGTAATTC CTACCATGAT TAACATTGGC 
TCTGGCTTAG CGCTGTTTGG AGTGGCAACA GTGCTGTGTG ACATCGTTGT CCTGTACTGC 
ATGAAAAAGA GGTACTTCTA TCGGGAGAAG AAGTACAAAT ACGTGGAGGA TTATGAACTG 
GGCACAAGTG AGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989622.1
CDS	3..1160
Translation	MAACCGAVRGFLFEYDTPRIVLIRSRKVGLINRAVQLAILAYVIGWVFLWEKGYQETDSVVSSVTTKVKGVTMTNTSTLGSRIWDVADYVIPPQEKNAVFVMTNMIFTLNQSQSHCPELPDDNTECNNNSSCVPGYVSTHSNGIQTGACIPYNSSIKTCEVFAWCPVEDDYHIPNPAFLQGAENFTILVKNNIWYPKFNFSKRNILPTFSSSYLKNCIYDAQTDPFCPIFRLGKIVEAAGQNFQEMAVEGGVMGLQINWDCNLDRAASHCVPKYSFRRLDNKDSANTISPGYNFRFAKYYKDSSGIETRTLIKAYGIRLDIIVFGKAGKFDVIPTMINIGSGLALFGVATVLCDIVVLYCMKKRYFYREKKYKYVEDYELGTSET

Target ORF information:

RefSeq Version	NM_204291.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus purinergic receptor P2X 4 (P2RX4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204291.1

ORF Insert Sequence:

ATGGCTGCGT GCTGCGGGGC TGTGCGCGGC TTCCTCTTCG AGTACGACAC CCCCCGCATC 
GTGCTGATCC GCAGCCGCAA AGTCGGGCTG ATCAACCGCG CCGTGCAGCT CGCCATCCTC 
GCCTACGTGA TCGGCTGGGT CTTTCTGTGG GAGAAGGGCT ACCAGGAAAC AGATTCCGTG 
GTCAGTTCTG TAACCACGAA GGTGAAAGGG GTAACGATGA CAAACACATC CACCTTGGGA 
TCCAGGATCT GGGATGTAGC TGACTACGTC ATCCCTCCCC AGGAGAAGAA CGCTGTGTTT 
GTCATGACCA ACATGATATT CACACTGAAC CAGAGCCAAA GCCACTGCCC AGAGCTTCCA 
GATGATAACA CAGAGTGCAA CAACAACAGC AGCTGCGTTC CAGGATATGT CAGCACCCAC 
AGCAATGGTA TCCAGACCGG GGCGTGCATA CCGTACAACA GCAGCATCAA GACCTGTGAA 
GTCTTTGCAT GGTGCCCTGT GGAGGATGAC TATCACATAC CCAATCCGGC ATTCTTGCAA 
GGAGCTGAGA ACTTCACCAT TCTGGTAAAG AACAACATTT GGTATCCCAA GTTCAACTTC 
AGCAAGCGAA ACATCCTCCC CACTTTCAGT TCCTCTTATC TCAAGAACTG CATCTATGAT 
GCCCAGACAG ATCCCTTCTG CCCTATCTTT CGTCTGGGGA AAATAGTTGA AGCTGCAGGG 
CAGAACTTCC AGGAAATGGC TGTGGAGGGT GGGGTCATGG GGCTGCAGAT CAACTGGGAC 
TGCAACCTCG ACAGAGCTGC TTCCCACTGC GTGCCCAAGT ATTCCTTCCG GCGCCTGGAC 
AACAAGGACT CTGCCAACAC TATCTCACCT GGGTACAACT TCAGGTTTGC AAAATACTAC 
AAGGATAGTA GTGGCATTGA AACACGAACG CTTATCAAAG CTTATGGCAT CCGCCTTGAT 
ATCATAGTGT TTGGAAAGGC AGGAAAATTT GATGTAATTC CTACCATGAT TAACATTGGC 
TCTGGCTTAG CGCTGTTTGG AGTGGCAACA GTGCTGTGTG ACATCGTTGT CCTGTACTGC 
ATGAAAAAGA GGTACTTCTA TCGGGAGAAG AAGTACAAAT ACGTGGAGGA TTATGAACTG 
GGCACAAGTG AGACATAA