LOC18016490 cDNA ORF clone, Eutrema salsugineum

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18016490
Entrez Gene ID	18016490
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Eutrema salsugineum

mRNA and Protein(s)

mRNA	Protein	Name
XM_006401640.2	XP_006401703.1	glutamate synthase 1 [NADH], chloroplastic isoform X2
XM_024155707.1	XP_024011475.1	glutamate synthase 1 [NADH], chloroplastic isoform X1
XM_006401640.1	XP_006401703.1	hypothetical protein
XM_006401641.1	XP_006401704.1	hypothetical protein
XM_024155708.1	XP_024011476.1	glutamate synthase 1 [NADH], chloroplastic isoform X3
XM_006401641.2	XP_006401704.2	glutamate synthase 1 [NADH], chloroplastic isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Reference Genome of the Halophytic Plant Eutrema salsugineum.
Yang R, Jarvis DE, Chen H, Beilstein MA, Grimwood J, Jenkins J, Shu S, Prochnik S, Xin M, Ma C, Schmutz J, Wing RA, Mitchell-Olds T, Schumaker KS, Wang X
Frontiers in plant science446(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC18016490 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18016490 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OEu134723	XM_006401641.2 Latest version!	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OEu134723	XM_024155708.1 Latest version!	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OEu60339	XM_006401640.2 Latest version!	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OEu60340	XM_006401641.1 Latest version!	Eutrema salsugineum hypothetical protein (EUTSA_v10012414mg) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OEu134722	XM_024155707.1 Latest version!	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OEu60339	XM_006401640.1	Eutrema salsugineum hypothetical protein (EUTSA_v10012414mg) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18016490 ( XM_024155708.1 ) cDNA ORF clone, Eutrema salsugineum -> XP_024011476.1 Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X4, mRNA.

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CloneID	OEu134723
Clone ID Related Accession (Same CDS sequence)	XM_024155708.1 , XM_006401641.2
Accession Version	XM_024155708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-25
Organism	Eutrema salsugineum
Product	glutamate synthase 1 [NADH], chloroplastic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006256829.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Eutrema salsugineum Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024011476.1
CDS	454..6054
Translation	MRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKNELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKAFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSQARPYGEWLERQKIELKDIIKSVPESERIASPISGVVPASDDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGTEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLTIEEMESIKKMNYRGWRTKVLDITYQKDQGTKGLEKTLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLLAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVFRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLARDALLLHEMAFPARGYAPGSAEASALSNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGFMKFKEADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLPDGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTLTEMIGRADMLELDREVVKNNDKLENIDLSLLLKPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPKDTIHIKFKGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFHTRCNLELVDLDKVEDEDDKMTLKMMIQQHQRHTDSQLAQEVLADFENLVPKFVKVFPRDYKRVLSAMKHEEVSNQAIERASEEADEMEEKELEEKDAFTELKNMAAASSKEEMSGNGVAAEAPKKPSLVNDAVKHRGFIAYEREGVKYRDPNVRLHDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFDEGWMVPRPPLKRTGRKVAIIGSGPAGLAAADQLNKMGHSVTVYERADRIGGLMMYGVPNMKTDKIDIVQRRVDLMTKEGINFVVNANIGKDPSFSLDGLKEENDAIVLAVGSTKPRDLPVSGRELSGVHFAMEFLHANTKSLLDSNLEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEAATKFGKDPRTYEVLTKRFIGDDNGNVKGLEIVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAASQVDKYLSKTDDDQADAKLRQSLNQMKHNDTRTS

Target ORF information:

RefSeq Version	XM_024155708.1
Organism	Eutrema salsugineum
Definition	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024155708.1

ORF Insert Sequence:

ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18016490 ( XM_006401641.2 ) cDNA ORF clone, Eutrema salsugineum -> XP_006401704.2 Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X3, mRNA.

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CloneID	OEu134723
Clone ID Related Accession (Same CDS sequence)	XM_024155708.1 , XM_006401641.2
Accession Version	XM_006401641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-25
Organism	Eutrema salsugineum
Product	glutamate synthase 1 [NADH], chloroplastic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006256829.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 26, 2018 this sequence version replaced XM_006401641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Eutrema salsugineum Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006401704.2
CDS	334..5934
Translation	MRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKNELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKAFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSQARPYGEWLERQKIELKDIIKSVPESERIASPISGVVPASDDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGTEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLTIEEMESIKKMNYRGWRTKVLDITYQKDQGTKGLEKTLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLLAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVFRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLARDALLLHEMAFPARGYAPGSAEASALSNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGFMKFKEADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLPDGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTLTEMIGRADMLELDREVVKNNDKLENIDLSLLLKPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPKDTIHIKFKGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFHTRCNLELVDLDKVEDEDDKMTLKMMIQQHQRHTDSQLAQEVLADFENLVPKFVKVFPRDYKRVLSAMKHEEVSNQAIERASEEADEMEEKELEEKDAFTELKNMAAASSKEEMSGNGVAAEAPKKPSLVNDAVKHRGFIAYEREGVKYRDPNVRLHDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFDEGWMVPRPPLKRTGRKVAIIGSGPAGLAAADQLNKMGHSVTVYERADRIGGLMMYGVPNMKTDKIDIVQRRVDLMTKEGINFVVNANIGKDPSFSLDGLKEENDAIVLAVGSTKPRDLPVSGRELSGVHFAMEFLHANTKSLLDSNLEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEAATKFGKDPRTYEVLTKRFIGDDNGNVKGLEIVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAASQVDKYLSKTDDDQADAKLRQSLNQMKHNDTRTS

Target ORF information:

RefSeq Version	XM_006401641.2
Organism	Eutrema salsugineum
Definition	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006401641.2

ORF Insert Sequence:

ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18016490 ( XM_006401640.2 ) cDNA ORF clone, Eutrema salsugineum -> XP_006401703.1 Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEu60339
Clone ID Related Accession (Same CDS sequence)	XM_006401640.2 , XM_006401640.1
Accession Version	XM_006401640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-25
Organism	Eutrema salsugineum
Product	glutamate synthase 1 [NADH], chloroplastic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006256829.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 26, 2018 this sequence version replaced XM_006401640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Eutrema salsugineum Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTCC ACTCTCGATT CTCTACCAAC ACATTTCCCA GCTGGGATCG TGCTCAGCCA 
ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006401703.1
CDS	387..6047
Translation	MLVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKNELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKAFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSQARPYGEWLERQKIELKDIIKSVPESERIASPISGVVPASDDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGTEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLTIEEMESIKKMNYRGWRTKVLDITYQKDQGTKGLEKTLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLLAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVFRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLARDALLLHEMAFPARGYAPGSAEASALSNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGFMKFKEADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLPDGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTLTEMIGRADMLELDREVVKNNDKLENIDLSLLLKPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPKDTIHIKFKGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFHTRCNLELVDLDKVEDEDDKMTLKMMIQQHQRHTDSQLAQEVLADFENLVPKFVKVFPRDYKRVLSAMKHEEVSNQAIERASEEADEMEEKELEEKDAFTELKNMAAASSKEEMSGNGVAAEAPKKPSLVNDAVKHRGFIAYEREGVKYRDPNVRLHDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFDEGWMVPRPPLKRTGRKVAIIGSGPAGLAAADQLNKMGHSVTVYERADRIGGLMMYGVPNMKTDKIDIVQRRVDLMTKEGINFVVNANIGKDPSFSLDGLKEENDAIVLAVGSTKPRDLPVSGRELSGVHFAMEFLHANTKSLLDSNLEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEAATKFGKDPRTYEVLTKRFIGDDNGNVKGLEIVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAASQVDKYLSKTDDDQADAKLRQSLNQMKHNDTRTS

Target ORF information:

RefSeq Version	XM_006401640.2
Organism	Eutrema salsugineum
Definition	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006401640.2

ORF Insert Sequence:

ATGCTGGTCC ACTCTCGATT CTCTACCAAC ACATTTCCCA GCTGGGATCG TGCTCAGCCA 
ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18016490 ( XM_006401640.1 ) cDNA ORF clone, Eutrema salsugineum -> XP_006401703.1 Eutrema salsugineum hypothetical protein (EUTSA_v10012414mg) mRNA, complete cds.

Price & Availability↑

CloneID	OEu60339
Clone ID Related Accession (Same CDS sequence)	XM_006401640.2 , XM_006401640.1
Accession Version	XM_006401640.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-19
Organism	Eutrema salsugineum
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_006256829).

ATGCTGGTCC ACTCTCGATT CTCTACCAAC ACATTTCCCA GCTGGGATCG TGCTCAGCCA 
ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006401703.1
CDS	19..5679
Translation	MLVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKNELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKAFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSQARPYGEWLERQKIELKDIIKSVPESERIASPISGVVPASDDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGTEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLTIEEMESIKKMNYRGWRTKVLDITYQKDQGTKGLEKTLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLLAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVFRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLARDALLLHEMAFPARGYAPGSAEASALSNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGFMKFKEADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLPDGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTLTEMIGRADMLELDREVVKNNDKLENIDLSLLLKPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPKDTIHIKFKGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFHTRCNLELVDLDKVEDEDDKMTLKMMIQQHQRHTDSQLAQEVLADFENLVPKFVKVFPRDYKRVLSAMKHEEVSNQAIERASEEADEMEEKELEEKDAFTELKNMAAASSKEEMSGNGVAAEAPKKPSLVNDAVKHRGFIAYEREGVKYRDPNVRLHDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFDEGWMVPRPPLKRTGRKVAIIGSGPAGLAAADQLNKMGHSVTVYERADRIGGLMMYGVPNMKTDKIDIVQRRVDLMTKEGINFVVNANIGKDPSFSLDGLKEENDAIVLAVGSTKPRDLPVSGRELSGVHFAMEFLHANTKSLLDSNLEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEAATKFGKDPRTYEVLTKRFIGDDNGNVKGLEIVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAASQVDKYLSKTDDDQADAKLRQSLNQMKHNDTRTS

Target ORF information:

RefSeq Version	XM_006401640.1
Organism	Eutrema salsugineum
Definition	Eutrema salsugineum hypothetical protein (EUTSA_v10012414mg) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006401640.1

ORF Insert Sequence:

ATGCTGGTCC ACTCTCGATT CTCTACCAAC ACATTTCCCA GCTGGGATCG TGCTCAGCCA 
ATGCGGGTTT TGGGCCACAA CGGGGAGATT AATACGCTTA GAGGAAACGT GAACTGGATG 
AGGGCTCGTG AGGGCCTGCT TAAGTGCAAG GAACTTGGTT TATCAAAGAA CGAGTTGAAG 
AAGCTTCTAC CTATTGTGGA TGTTAGCTCT TCTGACTCAG GTGCTTTTGA TGGTGTTCTT 
GAGCTTCTTG TCCGAGCTGG CAGAAGTCTT CCTGAAGCTG TTATGATGAT GATCCCAGAA 
GCATGGCAGA ATGACAAAAA CATAGATCCA AGCAGGAAAG CATTTTATGA ATACTTATCT 
GCCCTAATGG AGCCATGGGA TGGACCTGCT CTCATCTCAT TTACCGACGG TCGCTACTTA 
GGTGCCACAT TGGACCGTAA TGGACTTCGT CCTGGTAGGT TCTACATTAC CCACAGTGGA 
AGGGTTATCA TGGCTAGTGA AGTTGGGGTA GTGGATGTAC CGCCTGAAGA TGTGATGAGG 
AAAGGAAGGC TTAACCCGGG CATGATGCTT CTTGTCGATT TTGAGAAGCA TATTGTTGTT 
GATGACGATG CCTTGAAGCA ACAGTATTCA CAGGCAAGGC CGTATGGTGA ATGGCTTGAA 
AGGCAAAAGA TTGAACTTAA AGACATCATT AAGTCAGTTC CGGAGAGTGA ACGGATTGCT 
TCTCCAATTT CTGGGGTTGT ACCGGCGTCT GATGATGATG ACTCAATGGA GAGCATGGGC 
ATTCATGGTT TATTGTCTCC ACTAAAAGCC TTTGGCTATA CAGTTGAGGC CTTGGAAATG 
CTGCTGCTTC CGATGGCTAA AGATGGCACT GAAGCACTTG GTTCTATGGG AAATGATACT 
CCTTTAGCTG TGATGTCAAA CAGAGAGAAA TTATGTTTTG AGTACTTCAA GCAAATGTTT 
GCTCAAGTAA CCAATCCTCC AATCGATCCC ATTCGTGAGA AAATTGTTAC TTCAATGGAG 
TGCATGATCG GTCCAGAAGG TGATCTAACC GAAACAACTG AAGAGCAATG TCACCGTCTC 
TCCCTAAAAG GTCCTCTTTT GACCATAGAA GAGATGGAAT CAATCAAGAA AATGAATTAC 
AGAGGCTGGA GGACTAAAGT TCTTGATATA ACGTATCAAA AAGACCAAGG TACAAAAGGG 
TTGGAGAAGA CATTGGACCG AATTTGTGAT GAAGCAAATG AAGCGATAAA GGAGGGCTAC 
ACATTACTGG TTTTGTCTGA TCGAGCTTTC TCCGCTACGC GAGTTGCTGT GAGCTCTCTC 
TTGGCTGTTG GTGCCGTCCA TCACCACCTG GTTAAGACAC TTGCGCGCAC TCAAGTTGGT 
TTGGTAGTGG AATCTGCTGA ACCACGTGAA GTGCATCATT TCTGCACACT TGTCGGATTC 
GGAGCAGATG CTATTTGCCC ATATTTGGCT GTTGAGGCCG TTTTTAGATT GCAGGTCGAT 
GGCAAGATAC CACCCAAATC TAATGGGGAA TTCCATTCGA AAGAGGAGCT GGTCAAGAAG 
TATTACAAGG CCAGTAACTA TGGAATGATG AAGGTTCTTG CCAAAATGGG AATTTCTACT 
CTGGCCTCAT ACAAGGGTGC TCAAATATTT GAAGCTCTTG GGCTTTCGTC TGAAGTAATA 
GAGAAGTGTT TTGCTGGCAC CCCAAGCAGA GTGGAAGGAG CAACATTTGA GATGCTTGCA 
CGAGATGCGC TTCTTTTGCA TGAGATGGCC TTTCCAGCCC GTGGCTATGC CCCTGGAAGT 
GCTGAAGCCT CGGCACTTTC CAACCCTGGG AACTATCACT GGAGGAAAAA TGGGGAGATT 
CATCTCAATG ATCCTCTGGC GATTGCAAAA CTGCAAGAGG CAGCACGGAC TAACAGTGTC 
GCTGCCTACA AAGAATACTC GAAGCGTATA AATGAATTGA ACAAGCAAAG TAACTTGCGC 
GGGTTTATGA AGTTCAAAGA AGCAGATGTA AAGATTCCTC TAGATGAAGT GGAGCCTGCC 
AGTGAAATCG TTAAACGGTT CTGCACTGGC GCCATGAGTT ATGGATCAAT ATCACTGGAG 
GCGCATACAA CCCTTGCAAT GGCCATGAAC AAACTTGGAG GAAAGTCCAA TACAGGTGAG 
GGAGGGGAAC TGCCATCCCG TATGGAGCCT CTTCCAGATG GTTCAAGGAA TCCGAAAAGA 
AGTTCCATCA AACAAATTGC TAGTGGAAGA TTTGGAGTTT CGAGCTACTA TCTTACTAAT 
GCCGATGAGC TGCAGATAAA GATGGCTCAG GGTGCTAAAC CTGGTGAAGG AGGCGAGCTA 
CCTGGCCACA AGGTTATAGG AGACATCGCT ATCACCAGAA ATTCCACCGC CGGTGTGGGG 
CTTATCAGTC CACCTCCTCA TCATGATATC TACTCTATCG AGGATCTTGC CCAACTAATT 
CATGATCTTA AGAATGCCAA CCCTGGGGCC CGTATTAGTG TTAAACTTGT ATCGGAAGCT 
GGTGTTGGAG TTATTGCTAG CGGTGTGGTG AAAGGCCACG CTGACCATGT TCTGATAGCA 
GGTCATGATG GAGGTACTGG TGCATCTCGC TGGACCGGTA TAAAAAATGC TGGTCTTCCG 
TGGGAACTTG GTCTAGCTGA GACACACCAA ACTCTTGTCG CTAATGACCT TCGTGGTCGT 
ACTGTTCTTC AGACAGATGG CCAACTGAAA ACTGGCAGAG ATGTTGCAGT TGCTGCGCTT 
CTTGGTGCAG AAGAGTTTGG TTTTAGTACT GCACCCCTTA TTACACTCGG GTGTATCATG 
ATGAGAAAAT GCCACAAGAA CACCTGCCCT GTGGGAATTG CTACTCAAGA TCCAGTTCTC 
CGTGAAAAAT TTGCTGGGGA GCCTGAACAT GTCATTAATT TCTTCTTTAT GCTTGCTGAG 
GAAGTTAGGG AGATCATGTC TGGACTTGGT TTCCGAACTC TAACTGAAAT GATTGGTCGG 
GCAGATATGC TTGAACTTGA CAGGGAAGTG GTCAAGAACA ATGATAAGCT GGAGAACATT 
GACCTCTCTC TTTTGCTCAA ACCCGCTGCT GAGATTCGTC CTGGGGCTGC TCAGTACTGT 
GTCCAGAAAC AAGATCATGG CTTAGACATG GCTTTGGATC AAGAGCTTAT TGCATTGTCC 
AAGTCTGCTC TAGAGAAATC TCTTCCGGTG TACATTGAAA CTCCTATCTG CAACGTCAAC 
CGTGCTGTTG GGACTATGCT GAGCCATGAG GTTACCAAAC GCTATCACTT GGCTGGGCTA 
CCAAAGGATA CAATTCATAT CAAGTTCAAA GGAAGTGCTG GGCAGAGTCT TGGTGCTTTC 
CTGTGTCCTG GAATTACGCT AGAACTGGAG GGTGATAGCA ACGACTATGT TGGAAAAGGT 
CTCTCCGGAG GAAAAGTCGT TGTCTACCCT CCTAAAGGAA GCAGCTTTGA TCCAAAAGAA 
AACATTGTTA TCGGTAACGT AGCACTTTAC GGGGCCACTA GTGGTGAAGC CTACTTTAAT 
GGGATGGCTG CTGAAAGATT CTCTGTTCGT AATTCTGGTG CCAAAGCTGT TGTGGAAGGT 
GTTGGTGATC ATGGTTGTGA GTACATGACT GGTGGTACTG TTGTGGTGCT TGGAAAGACT 
GGTAGAAACT TTGCTGCTGG TATGAGTGGT GGTATTGCTT ATGTCCTTGA TGTGGATGGA 
AAGTTTCACA CGAGATGCAA CCTTGAACTG GTTGATCTGG ACAAAGTTGA AGATGAAGAC 
GACAAAATGA CGCTAAAAAT GATGATCCAG CAACATCAGC GACACACAGA CAGCCAACTT 
GCTCAAGAAG TTCTTGCGGA CTTTGAGAAT TTGGTACCGA AGTTTGTCAA GGTTTTCCCA 
AGAGATTACA AACGTGTTTT ATCAGCCATG AAACATGAAG AGGTCTCGAA TCAAGCCATA 
GAGCGTGCTT CTGAAGAAGC TGACGAGATG GAAGAGAAAG AACTAGAGGA GAAAGACGCC 
TTTACAGAGC TGAAGAACAT GGCAGCTGCC TCTTCAAAAG AGGAGATGTC AGGAAACGGC 
GTGGCAGCTG AAGCTCCGAA GAAACCTTCT CTGGTAAATG ATGCTGTTAA ACATCGTGGT 
TTCATTGCTT ATGAACGTGA GGGAGTTAAG TACAGGGACC CTAATGTTCG TCTTCATGAC 
TGGAACGAAG TCATGGAGGA ATCAAAACCT GGACCGCTCC TTACAACTCA GTCTGCTCGC 
TGCATGGACT GTGGAACTCC ATTCTGCCAC CAGGAGAACT CTGGGTGTCC GCTTGGTAAC 
AAGATCCCTG AATTCAATGA ACTTGTCTAC CAGAACAGAT GGCAAGAAGC GTTGAACCGT 
CTACTTGAGA CAAATAACTT TCCCGAATTC ACTGGGCGAG TTTGCCCTGC ACCATGTGAA 
GGTTCTTGTG TTCTTGGAAT AATTGAGAAC CCTGTTTCTA TCAAAAGCAT CGAATGTGCT 
ATCATAGATA AAGCCTTTGA TGAAGGGTGG ATGGTACCAA GACCTCCTCT CAAGAGAACA 
GGGAGAAAAG TTGCAATTAT CGGAAGTGGG CCGGCTGGAC TGGCTGCTGC TGACCAGCTG 
AACAAAATGG GTCACTCGGT AACCGTGTAT GAGCGTGCTG ACAGAATTGG CGGGCTTATG 
ATGTATGGAG TCCCAAACAT GAAGACTGAC AAAATTGACA TAGTTCAACG TCGGGTTGAT 
CTTATGACCA AAGAAGGTAT CAACTTTGTG GTCAATGCCA ATATTGGAAA AGACCCGTCT 
TTCTCCCTGG ATGGACTTAA GGAGGAGAAT GATGCAATCG TTCTTGCTGT TGGTTCCACA 
AAACCAAGAG ATCTTCCAGT GTCTGGTCGT GAGCTATCTG GTGTTCACTT TGCCATGGAG 
TTTCTTCATG CAAACACCAA AAGCTTGCTT GATAGCAATC TTGAAGATGG CAATTACATT 
TCCGCAAAGG GGAAAAAGGT TGTCGTTATT GGTGGAGGTG ATACGGGCAC AGATTGCATT 
GGAACATCTA TCCGCCATGG ATGCACCAAC ATTGTAAACC TAGAGCTTCT TCCTCAGCCA 
CCTTCAACGA GAGCTCCAGG AAACCCTTGG CCACAGTGGC CTCGTGTATT CCGTATTGAC 
TACGGACATC AAGAAGCAGC GACCAAATTC GGAAAGGACC CTAGAACCTA TGAGGTCTTA 
ACTAAGCGAT TCATAGGAGA TGACAATGGA AACGTGAAAG GGCTAGAGAT TGTGCGCGTG 
AGTTGGGAGA AGGATGAAAC CGGGAGGTTC CAATTCAAGG AGATCGAAGG CTCCGAGGAA 
ATAATTGAAG CGGACCTTGT TTTCTTAGCC ATGGGATTCC TTGGACCTGA GCCAACACTT 
GCCGAGAAAC TAGGACTCGA ATGTGACAAC AGGTCGAACT TTAAGGCAGA GTATGGCCGG 
TTTTCAACAA CAGTGGAAGG TGTTTTTGCT GCAGGTGATT GTCGAAGAGG ACAGTCTTTG 
GTGGTCTGGG CCATCTCAGA AGGACGCCAA GCTGCATCTC AGGTCGACAA ATACCTCAGC 
AAAACTGATG ACGACCAAGC AGATGCCAAG CTACGACAGA GCCTCAACCA GATGAAGCAC 
AACGACACTA GGACGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18016490 ( XM_006401641.1 ) cDNA ORF clone, Eutrema salsugineum -> XP_006401704.1 Eutrema salsugineum hypothetical protein (EUTSA_v10012414mg) mRNA, complete cds.

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CloneID	OEu60340
Clone ID Related Accession (Same CDS sequence)	XM_006401641.1
Accession Version	XM_006401641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-19
Organism	Eutrema salsugineum
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_006256829). COMPLETENESS: incomplete on the 3' end.

ATGATTGACT ATGCTCGGTC TACATGTTTA ATTGTTCAGG TCCACTCTCG ATTCTCTACC 
AACACATTTC CCAGCTGGGA TCGTGCTCAG CCAATGCGGG TTTTGGGCCA CAACGGGGAG 
ATTAATACGC TTAGAGGAAA CGTGAACTGG ATGAGGGCTC GTGAGGGCCT GCTTAAGTGC 
AAGGAACTTG GTTTATCAAA GAACGAGTTG AAGAAGCTTC TACCTATTGT GGATGTTAGC 
TCTTCTGACT CAGGTGCTTT TGATGGTGTT CTTGAGCTTC TTGTCCGAGC TGGCAGAAGT 
CTTCCTGAAG CTGTTATGAT GATGATCCCA GAAGCATGGC AGAATGACAA AAACATAGAT 
CCAAGCAGGA AAGCATTTTA TGAATACTTA TCTGCCCTAA TGGAGCCATG GGATGGACCT 
GCTCTCATCT CATTTACCGA CGGTCGCTAC TTAGGTGCCA CATTGGACCG TAATGGACTT 
CGTCCTGGTA GGTTCTACAT TACCCACAGT GGAAGGGTTA TCATGGCTAG TGAAGTTGGG 
GTAGTGGATG TACCGCCTGA AGATGTGATG AGGAAAGGAA GGCTTAACCC GGGCATGATG 
CTTCTTGTCG ATTTTGAGAA GCATATTGTT GTTGATGACG ATGCCTTGAA GCAACAGTAT 
TCACAGGCAA GGCCGTATGG TGAATGGCTT GAAAGGCAAA AGATTGAACT TAAAGACATC 
ATTAAGTCAG TTCCGGAGAG TGAACGGATT GCTTCTCCAA TTTCTGGGGT TGTACCGGCG 
TCTGATGATG ATGACTCAAT GGAGAGCATG GGCATTCATG GTTTATTGTC TCCACTAAAA 
GCCTTTGGCT ATACAGTTGA GGCCTTGGAA ATGCTGCTGC TTCCGATGGC TAAAGATGGC 
ACTGAAGCAC TTGGTTCTAT GGGAAATGAT ACTCCTTTAG CTGTGATGTC AAACAGAGAG 
AAATTATGTT TTGAGTACTT CAAGCAAATG TTTGCTCAAG TAACCAATCC TCCAATCGAT 
CCCATTCGTG AGAAAATTGT TACTTCAATG GAGTGCATGA TCGGTCCAGA AGGTGATCTA 
ACCGAAACAA CTGAAGAGCA ATGTCACCGT CTCTCCCTAA AAGGTCCTCT TTTGACCATA 
GAAGAGATGG AATCAATCAA GAAAATGAAT TACAGAGGCT GGAGGACTAA AGTTCTTGAT 
ATAACGTATC AAAAAGACCA AGGTACAAAA GGGTTGGAGA AGACATTGGA CCGAATTTGT 
GATGAAGCAA ATGAAGCGAT AAAGGAGGGC TACACATTAC TGGTTTTGTC TGATCGAGCT 
TTCTCCGCTA CGCGAGTTGC TGTGAGCTCT CTCTTGGCTG TTGGTGCCGT CCATCACCAC 
CTGGTTAAGA CACTTGCGCG CACTCAAGTT GGTTTGGTAG TGGAATCTGC TGAACCACGT 
GAAGTGCATC ATTTCTGCAC ACTTGTCGGA TTCGGAGCAG ATGCTATTTG CCCATATTTG 
GCTGTTGAGG CCGTTTTTAG ATTGCAGGTC GATGGCAAGA TACCACCCAA ATCTAATGGG 
GAATTCCATT CGAAAGAGGA GCTGGTCAAG AAGTATTACA AGGCCAGTAA CTATGGAATG 
ATGAAGGTTC TTGCCAAAAT GGGAATTTCT ACTCTGGCCT CATACAAGGG TGCTCAAATA 
TTTGAAGCTC TTGGGCTTTC GTCTGAAGTA ATAGAGAAGT GTTTTGCTGG CACCCCAAGC 
AGAGTGGAAG GAGCAACATT TGAGATGCTT GCACGAGATG CGCTTCTTTT GCATGAGATG 
GCCTTTCCAG CCCGTGGCTA TGCCCCTGGA AGTGCTGAAG CCTCGGCACT TTCCAACCCT 
GGGAACTATC ACTGGAGGAA AAATGGGGAG ATTCATCTCA ATGATCCTCT GGCGATTGCA 
AAACTGCAAG AGGCAGCACG GACTAACAGT GTCGCTGCCT ACAAAGAATA CTCGAAGCGT 
ATAAATGAAT TGAACAAGCA AAGTAACTTG CGCGGGTTTA TGAAGTTCAA AGAAGCAGAT 
GTAAAGATTC CTCTAGATGA AGTGGAGCCT GCCAGTGAAA TCGTTAAACG GTTCTGCACT 
GGCGCCATGA GTTATGGATC AATATCACTG GAGGCGCATA CAACCCTTGC AATGGCCATG 
AACAAACTTG GAGGAAAGTC CAATACAGGT GAGGGAGGGG AACTGCCATC CCGTATGGAG 
CCTCTTCCAG ATGGTTCAAG GAATCCGAAA AGAAGTTCCA TCAAACAAAT TGCTAGTGGA 
AGATTTGGAG TTTCGAGCTA CTATCTTACT AATGCCGATG AGCTGCAGAT AAAGATGGCT 
CAGGGTGCTA AACCTGGTGA AGGAGGCGAG CTACCTGGCC ACAAGGTTAT AGGAGACATC 
GCTATCACCA GAAATTCCAC CGCCGGTGTG GGGCTTATCA GTCCACCTCC TCATCATGAT 
ATCTACTCTA TCGAGGATCT TGCCCAACTA ATTCATGATC TTAAGAATGC CAACCCTGGG 
GCCCGTATTA GTGTTAAACT TGTATCGGAA GCTGGTGTTG GAGTTATTGC TAGCGGTGTG 
GTGAAAGGCC ACGCTGACCA TGTTCTGATA GCAGGTCATG ATGGAGGTAC TGGTGCATCT 
CGCTGGACCG GTATAAAAAA TGCTGGTCTT CCGTGGGAAC TTGGTCTAGC TGAGACACAC 
CAAACTCTTG TCGCTAATGA CCTTCGTGGT CGTACTGTTC TTCAGACAGA TGGCCAACTG 
AAAACTGGCA GAGATGTTGC AGTTGCTGCG CTTCTTGGTG CAGAAGAGTT TGGTTTTAGT 
ACTGCACCCC TTATTACACT CGGGTGTATC ATGATGAGAA AATGCCACAA GAACACCTGC 
CCTGTGGGAA TTGCTACTCA AGATCCAGTT CTCCGTGAAA AATTTGCTGG GGAGCCTGAA 
CATGTCATTA ATTTCTTCTT TATGCTTGCT GAGGAAGTTA GGGAGATCAT GTCTGGACTT 
GGTTTCCGAA CTCTAACTGA AATGATTGGT CGGGCAGATA TGCTTGAACT TGACAGGGAA 
GTGGTCAAGA ACAATGATAA GCTGGAGAAC ATTGACCTCT CTCTTTTGCT CAAACCCGCT 
GCTGAGATTC GTCCTGGGGC TGCTCAGTAC TGTGTCCAGA AACAAGATCA TGGCTTAGAC 
ATGGCTTTGG ATCAAGAGCT TATTGCATTG TCCAAGTCTG CTCTAGAGAA ATCTCTTCCG 
GTGTACATTG AAACTCCTAT CTGCAACGTC AACCGTGCTG TTGGGACTAT GCTGAGCCAT 
GAGGTTACCA AACGCTATCA CTTGGCTGGG CTACCAAAGG ATACAATTCA TATCAAGTTC 
AAAGGAAGTG CTGGGCAGAG TCTTGGTGCT TTCCTGTGTC CTGGAATTAC GCTAGAACTG 
GAGGGTGATA GCAACGACTA TGTTGGAAAA GGTCTCTCCG GAGGAAAAGT CGTTGTCTAC 
CCTCCTAAAG GAAGCAGCTT TGATCCAAAA GAAAACATTG TTATCGGTAA CGTAGCACTT 
TACGGGGCCA CTAGTGGTGA AGCCTACTTT AATGGGATGG CTGCTGAAAG ATTCTCTGTT 
CGTAATTCTG GTGCCAAAGC TGTTGTGGAA GGTGTTGGTG ATCATGGTTG TGAGTACATG 
ACTGGTGGTA CTGTTGTGGT GCTTGGAAAG ACTGGTAGAA ACTTTGCTGC TGGTATGAGT 
GGTGGTATTG CTTATGTCCT TGATGTGGAT GGAAAGTTTC ACACGAGATG CAACCTTGAA 
CTGGTTGATC TGGACAAAGT TGAAGATGAA GACGACAAAA TGACGCTAAA AATGATGATC 
CAGCAACATC AGCGACACAC AGACAGCCAA CTTGCTCAAG AAGTTCTTGC GGACTTTGAG 
AATTTGGTAC CGAAGTTTGT CAAGGTTTTC CCAAGAGATT ACAAACGTGT TTTATCAGCC 
ATGAAACATG AAGAGGTCTC GAATCAAGCC ATAGAGCGTG CTTCTGAAGA AGCTGACGAG 
ATGGAAGAGA AAGAACTAGA GGAGAAAGAC GCCTTTACAG AGCTGAAGAA CATGGCAGCT 
GCCTCTTCAA AAGAGGAGAT GTCAGGAAAC GGCGTGGCAG CTGAAGCTCC GAAGAAACCT 
TCTCTGGTAA ATGATGCTGT TAAACATCGT GGTTTCATTG CTTATGAACG TGAGGGAGTT 
AAGTACAGGG ACCCTAATGT TCGTCTTCAT GACTGGAACG AAGTCATGGA GGAATCAAAA 
CCTGGACCGC TCCTTACAAC TCAGTCTGCT CGCTGCATGG ACTGTGGAAC TCCATTCTGC 
CACCAGGAGA ACTCTGGGTG TCCGCTTGGT AACAAGATCC CTGAATTCAA TGAACTTGTC 
TACCAGAACA GATGGCAAGA AGCGTTGAAC CGTCTACTTG AGACAAATAA CTTTCCCGAA 
TTCACTGGGC GAGTTTGCCC TGCACCATGT GAAGGTTCTT GTGTTCTTGG AATAATTGAG 
AACCCTGTTT CTATCAAAAG CATCGAATGT GCTATCATAG ATAAAGCCTT TGATGAAGGG 
TGGATGGTAC CAAGACCTCC TCTCAAGAGA ACAGGGAGAA AAGTTGCAAT TATCGGAAGT 
GGGCCGGCTG GACTGGCTGC TGCTGACCAG CTGAACAAAA TGGGTCACTC GGTAACCGTG 
TATGAGCGTG CTGACAGAAT TGGCGGGCTT ATGATGTATG GAGTCCCAAA CATGAAGACT 
GACAAAATTG ACATAGTTCA ACGTCGGGTT GATCTTATGA CCAAAGAAGG TATCAACTTT 
GTGGTCAATG CCAATATTGG AAAAGACCCG TCTTTCTCCC TGGATGGACT TAAGGAGGAG 
AATGATGCAA TCGTTCTTGC TGTTGGTTCC ACAAAACCAA GAGATCTTCC AGTGTCTGGT 
CGTGAGCTAT CTGGTGTTCA CTTTGCCATG GAGTTTCTTC ATGCAAACAC CAAAAGCTTG 
CTTGATAGCA ATCTTGAAGA TGGCAATTAC ATTTCCGCAA AGGGGAAAAA GGTTGTCGTT 
ATTGGTGGAG GTGATACGGG CACAGATTGC ATTGGAACAT CTATCCGCCA TGGATGCACC 
AACATTGTAA ACCTAGAGCT TCTTCCTCAG CCACCTTCAA CGAGAGCTCC AGGAAACCCT 
TGGCCACAGT GGCCTCGTGT ATTCCGTATT GACTACGGAC ATCAAGAAGC AGCGACCAAA 
TTCGGAAAGG ACCCTAGAAC CTATGAGGTC TTAACTAAGC GATTCATAGG AGATGACAAT 
GGAAACGTGA AAGGGCTAGA GATTGTGCGC GTGAGTTGGG AGAAGGATGA AACCGGGAGG 
TTCCAATTCA AGGAGATCGA AGGCTCCGAG GAAATAATTG AAGCGGACCT TGTTTTCTTA 
GCCATGGGAT TCCTTGGACC TGAGCCAACA CTTGCCGAGA AACTAGGACT CGAATGTGAC 
AACAGGTCGA ACTTTAAGGC AGAGTATGGC CGGTTTTCAA CAACAGTGGA AGGTGTTTTT 
GCTGCAGGTG ATTGTCGAAG AGGACAGTCT TTGGTGGTCT GGGCCATCTC AGAAGGACGC 
CAAGCTGCAT CTCAGGTCGA CAAATACCTC AGCAAAACTG ATGACGACCA AGCAGATGCC 
AAGCTACGAC AGAGCCTCAA CCAGATGAAG CACAACGACA CTAGGACGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006401704.1
CDS	64..5757
Translation	MIDYARSTCLIVQVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKNELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKAFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSQARPYGEWLERQKIELKDIIKSVPESERIASPISGVVPASDDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGTEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLTIEEMESIKKMNYRGWRTKVLDITYQKDQGTKGLEKTLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLLAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVFRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLARDALLLHEMAFPARGYAPGSAEASALSNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGFMKFKEADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLPDGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTLTEMIGRADMLELDREVVKNNDKLENIDLSLLLKPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPKDTIHIKFKGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFHTRCNLELVDLDKVEDEDDKMTLKMMIQQHQRHTDSQLAQEVLADFENLVPKFVKVFPRDYKRVLSAMKHEEVSNQAIERASEEADEMEEKELEEKDAFTELKNMAAASSKEEMSGNGVAAEAPKKPSLVNDAVKHRGFIAYEREGVKYRDPNVRLHDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFDEGWMVPRPPLKRTGRKVAIIGSGPAGLAAADQLNKMGHSVTVYERADRIGGLMMYGVPNMKTDKIDIVQRRVDLMTKEGINFVVNANIGKDPSFSLDGLKEENDAIVLAVGSTKPRDLPVSGRELSGVHFAMEFLHANTKSLLDSNLEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEAATKFGKDPRTYEVLTKRFIGDDNGNVKGLEIVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAASQVDKYLSKTDDDQADAKLRQSLNQMKHNDTRTS

Target ORF information:

RefSeq Version	XM_006401641.1
Organism	Eutrema salsugineum
Definition	Eutrema salsugineum hypothetical protein (EUTSA_v10012414mg) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006401641.1

ORF Insert Sequence:

ATGATTGACT ATGCTCGGTC TACATGTTTA ATTGTTCAGG TCCACTCTCG ATTCTCTACC 
AACACATTTC CCAGCTGGGA TCGTGCTCAG CCAATGCGGG TTTTGGGCCA CAACGGGGAG 
ATTAATACGC TTAGAGGAAA CGTGAACTGG ATGAGGGCTC GTGAGGGCCT GCTTAAGTGC 
AAGGAACTTG GTTTATCAAA GAACGAGTTG AAGAAGCTTC TACCTATTGT GGATGTTAGC 
TCTTCTGACT CAGGTGCTTT TGATGGTGTT CTTGAGCTTC TTGTCCGAGC TGGCAGAAGT 
CTTCCTGAAG CTGTTATGAT GATGATCCCA GAAGCATGGC AGAATGACAA AAACATAGAT 
CCAAGCAGGA AAGCATTTTA TGAATACTTA TCTGCCCTAA TGGAGCCATG GGATGGACCT 
GCTCTCATCT CATTTACCGA CGGTCGCTAC TTAGGTGCCA CATTGGACCG TAATGGACTT 
CGTCCTGGTA GGTTCTACAT TACCCACAGT GGAAGGGTTA TCATGGCTAG TGAAGTTGGG 
GTAGTGGATG TACCGCCTGA AGATGTGATG AGGAAAGGAA GGCTTAACCC GGGCATGATG 
CTTCTTGTCG ATTTTGAGAA GCATATTGTT GTTGATGACG ATGCCTTGAA GCAACAGTAT 
TCACAGGCAA GGCCGTATGG TGAATGGCTT GAAAGGCAAA AGATTGAACT TAAAGACATC 
ATTAAGTCAG TTCCGGAGAG TGAACGGATT GCTTCTCCAA TTTCTGGGGT TGTACCGGCG 
TCTGATGATG ATGACTCAAT GGAGAGCATG GGCATTCATG GTTTATTGTC TCCACTAAAA 
GCCTTTGGCT ATACAGTTGA GGCCTTGGAA ATGCTGCTGC TTCCGATGGC TAAAGATGGC 
ACTGAAGCAC TTGGTTCTAT GGGAAATGAT ACTCCTTTAG CTGTGATGTC AAACAGAGAG 
AAATTATGTT TTGAGTACTT CAAGCAAATG TTTGCTCAAG TAACCAATCC TCCAATCGAT 
CCCATTCGTG AGAAAATTGT TACTTCAATG GAGTGCATGA TCGGTCCAGA AGGTGATCTA 
ACCGAAACAA CTGAAGAGCA ATGTCACCGT CTCTCCCTAA AAGGTCCTCT TTTGACCATA 
GAAGAGATGG AATCAATCAA GAAAATGAAT TACAGAGGCT GGAGGACTAA AGTTCTTGAT 
ATAACGTATC AAAAAGACCA AGGTACAAAA GGGTTGGAGA AGACATTGGA CCGAATTTGT 
GATGAAGCAA ATGAAGCGAT AAAGGAGGGC TACACATTAC TGGTTTTGTC TGATCGAGCT 
TTCTCCGCTA CGCGAGTTGC TGTGAGCTCT CTCTTGGCTG TTGGTGCCGT CCATCACCAC 
CTGGTTAAGA CACTTGCGCG CACTCAAGTT GGTTTGGTAG TGGAATCTGC TGAACCACGT 
GAAGTGCATC ATTTCTGCAC ACTTGTCGGA TTCGGAGCAG ATGCTATTTG CCCATATTTG 
GCTGTTGAGG CCGTTTTTAG ATTGCAGGTC GATGGCAAGA TACCACCCAA ATCTAATGGG 
GAATTCCATT CGAAAGAGGA GCTGGTCAAG AAGTATTACA AGGCCAGTAA CTATGGAATG 
ATGAAGGTTC TTGCCAAAAT GGGAATTTCT ACTCTGGCCT CATACAAGGG TGCTCAAATA 
TTTGAAGCTC TTGGGCTTTC GTCTGAAGTA ATAGAGAAGT GTTTTGCTGG CACCCCAAGC 
AGAGTGGAAG GAGCAACATT TGAGATGCTT GCACGAGATG CGCTTCTTTT GCATGAGATG 
GCCTTTCCAG CCCGTGGCTA TGCCCCTGGA AGTGCTGAAG CCTCGGCACT TTCCAACCCT 
GGGAACTATC ACTGGAGGAA AAATGGGGAG ATTCATCTCA ATGATCCTCT GGCGATTGCA 
AAACTGCAAG AGGCAGCACG GACTAACAGT GTCGCTGCCT ACAAAGAATA CTCGAAGCGT 
ATAAATGAAT TGAACAAGCA AAGTAACTTG CGCGGGTTTA TGAAGTTCAA AGAAGCAGAT 
GTAAAGATTC CTCTAGATGA AGTGGAGCCT GCCAGTGAAA TCGTTAAACG GTTCTGCACT 
GGCGCCATGA GTTATGGATC AATATCACTG GAGGCGCATA CAACCCTTGC AATGGCCATG 
AACAAACTTG GAGGAAAGTC CAATACAGGT GAGGGAGGGG AACTGCCATC CCGTATGGAG 
CCTCTTCCAG ATGGTTCAAG GAATCCGAAA AGAAGTTCCA TCAAACAAAT TGCTAGTGGA 
AGATTTGGAG TTTCGAGCTA CTATCTTACT AATGCCGATG AGCTGCAGAT AAAGATGGCT 
CAGGGTGCTA AACCTGGTGA AGGAGGCGAG CTACCTGGCC ACAAGGTTAT AGGAGACATC 
GCTATCACCA GAAATTCCAC CGCCGGTGTG GGGCTTATCA GTCCACCTCC TCATCATGAT 
ATCTACTCTA TCGAGGATCT TGCCCAACTA ATTCATGATC TTAAGAATGC CAACCCTGGG 
GCCCGTATTA GTGTTAAACT TGTATCGGAA GCTGGTGTTG GAGTTATTGC TAGCGGTGTG 
GTGAAAGGCC ACGCTGACCA TGTTCTGATA GCAGGTCATG ATGGAGGTAC TGGTGCATCT 
CGCTGGACCG GTATAAAAAA TGCTGGTCTT CCGTGGGAAC TTGGTCTAGC TGAGACACAC 
CAAACTCTTG TCGCTAATGA CCTTCGTGGT CGTACTGTTC TTCAGACAGA TGGCCAACTG 
AAAACTGGCA GAGATGTTGC AGTTGCTGCG CTTCTTGGTG CAGAAGAGTT TGGTTTTAGT 
ACTGCACCCC TTATTACACT CGGGTGTATC ATGATGAGAA AATGCCACAA GAACACCTGC 
CCTGTGGGAA TTGCTACTCA AGATCCAGTT CTCCGTGAAA AATTTGCTGG GGAGCCTGAA 
CATGTCATTA ATTTCTTCTT TATGCTTGCT GAGGAAGTTA GGGAGATCAT GTCTGGACTT 
GGTTTCCGAA CTCTAACTGA AATGATTGGT CGGGCAGATA TGCTTGAACT TGACAGGGAA 
GTGGTCAAGA ACAATGATAA GCTGGAGAAC ATTGACCTCT CTCTTTTGCT CAAACCCGCT 
GCTGAGATTC GTCCTGGGGC TGCTCAGTAC TGTGTCCAGA AACAAGATCA TGGCTTAGAC 
ATGGCTTTGG ATCAAGAGCT TATTGCATTG TCCAAGTCTG CTCTAGAGAA ATCTCTTCCG 
GTGTACATTG AAACTCCTAT CTGCAACGTC AACCGTGCTG TTGGGACTAT GCTGAGCCAT 
GAGGTTACCA AACGCTATCA CTTGGCTGGG CTACCAAAGG ATACAATTCA TATCAAGTTC 
AAAGGAAGTG CTGGGCAGAG TCTTGGTGCT TTCCTGTGTC CTGGAATTAC GCTAGAACTG 
GAGGGTGATA GCAACGACTA TGTTGGAAAA GGTCTCTCCG GAGGAAAAGT CGTTGTCTAC 
CCTCCTAAAG GAAGCAGCTT TGATCCAAAA GAAAACATTG TTATCGGTAA CGTAGCACTT 
TACGGGGCCA CTAGTGGTGA AGCCTACTTT AATGGGATGG CTGCTGAAAG ATTCTCTGTT 
CGTAATTCTG GTGCCAAAGC TGTTGTGGAA GGTGTTGGTG ATCATGGTTG TGAGTACATG 
ACTGGTGGTA CTGTTGTGGT GCTTGGAAAG ACTGGTAGAA ACTTTGCTGC TGGTATGAGT 
GGTGGTATTG CTTATGTCCT TGATGTGGAT GGAAAGTTTC ACACGAGATG CAACCTTGAA 
CTGGTTGATC TGGACAAAGT TGAAGATGAA GACGACAAAA TGACGCTAAA AATGATGATC 
CAGCAACATC AGCGACACAC AGACAGCCAA CTTGCTCAAG AAGTTCTTGC GGACTTTGAG 
AATTTGGTAC CGAAGTTTGT CAAGGTTTTC CCAAGAGATT ACAAACGTGT TTTATCAGCC 
ATGAAACATG AAGAGGTCTC GAATCAAGCC ATAGAGCGTG CTTCTGAAGA AGCTGACGAG 
ATGGAAGAGA AAGAACTAGA GGAGAAAGAC GCCTTTACAG AGCTGAAGAA CATGGCAGCT 
GCCTCTTCAA AAGAGGAGAT GTCAGGAAAC GGCGTGGCAG CTGAAGCTCC GAAGAAACCT 
TCTCTGGTAA ATGATGCTGT TAAACATCGT GGTTTCATTG CTTATGAACG TGAGGGAGTT 
AAGTACAGGG ACCCTAATGT TCGTCTTCAT GACTGGAACG AAGTCATGGA GGAATCAAAA 
CCTGGACCGC TCCTTACAAC TCAGTCTGCT CGCTGCATGG ACTGTGGAAC TCCATTCTGC 
CACCAGGAGA ACTCTGGGTG TCCGCTTGGT AACAAGATCC CTGAATTCAA TGAACTTGTC 
TACCAGAACA GATGGCAAGA AGCGTTGAAC CGTCTACTTG AGACAAATAA CTTTCCCGAA 
TTCACTGGGC GAGTTTGCCC TGCACCATGT GAAGGTTCTT GTGTTCTTGG AATAATTGAG 
AACCCTGTTT CTATCAAAAG CATCGAATGT GCTATCATAG ATAAAGCCTT TGATGAAGGG 
TGGATGGTAC CAAGACCTCC TCTCAAGAGA ACAGGGAGAA AAGTTGCAAT TATCGGAAGT 
GGGCCGGCTG GACTGGCTGC TGCTGACCAG CTGAACAAAA TGGGTCACTC GGTAACCGTG 
TATGAGCGTG CTGACAGAAT TGGCGGGCTT ATGATGTATG GAGTCCCAAA CATGAAGACT 
GACAAAATTG ACATAGTTCA ACGTCGGGTT GATCTTATGA CCAAAGAAGG TATCAACTTT 
GTGGTCAATG CCAATATTGG AAAAGACCCG TCTTTCTCCC TGGATGGACT TAAGGAGGAG 
AATGATGCAA TCGTTCTTGC TGTTGGTTCC ACAAAACCAA GAGATCTTCC AGTGTCTGGT 
CGTGAGCTAT CTGGTGTTCA CTTTGCCATG GAGTTTCTTC ATGCAAACAC CAAAAGCTTG 
CTTGATAGCA ATCTTGAAGA TGGCAATTAC ATTTCCGCAA AGGGGAAAAA GGTTGTCGTT 
ATTGGTGGAG GTGATACGGG CACAGATTGC ATTGGAACAT CTATCCGCCA TGGATGCACC 
AACATTGTAA ACCTAGAGCT TCTTCCTCAG CCACCTTCAA CGAGAGCTCC AGGAAACCCT 
TGGCCACAGT GGCCTCGTGT ATTCCGTATT GACTACGGAC ATCAAGAAGC AGCGACCAAA 
TTCGGAAAGG ACCCTAGAAC CTATGAGGTC TTAACTAAGC GATTCATAGG AGATGACAAT 
GGAAACGTGA AAGGGCTAGA GATTGTGCGC GTGAGTTGGG AGAAGGATGA AACCGGGAGG 
TTCCAATTCA AGGAGATCGA AGGCTCCGAG GAAATAATTG AAGCGGACCT TGTTTTCTTA 
GCCATGGGAT TCCTTGGACC TGAGCCAACA CTTGCCGAGA AACTAGGACT CGAATGTGAC 
AACAGGTCGA ACTTTAAGGC AGAGTATGGC CGGTTTTCAA CAACAGTGGA AGGTGTTTTT 
GCTGCAGGTG ATTGTCGAAG AGGACAGTCT TTGGTGGTCT GGGCCATCTC AGAAGGACGC 
CAAGCTGCAT CTCAGGTCGA CAAATACCTC AGCAAAACTG ATGACGACCA AGCAGATGCC 
AAGCTACGAC AGAGCCTCAA CCAGATGAAG CACAACGACA CTAGGACGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18016490 ( XM_024155707.1 ) cDNA ORF clone, Eutrema salsugineum -> XP_024011475.1 Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X1, mRNA.

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CloneID	OEu134722
Clone ID Related Accession (Same CDS sequence)	XM_024155707.1
Accession Version	XM_024155707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-25
Organism	Eutrema salsugineum
Product	glutamate synthase 1 [NADH], chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006256829.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Eutrema salsugineum Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCAG CTTCTTCAAG CTCCGTTCTC CACCTCCGAA ACAATCAGTT GTTACCAATC 
CGTTCTCTGA AAAATCCCAC CGTCGTTGCT TCTCAATTGG CCGTCGCGCC TGGCGTCGGT 
CGGAGACGAG CATGTACCGC ACGATGCTCC GTGAAGAAAC CGGCGGCTCC CGAGAGTCCT 
TTCCTGGGTA CTCGGATGAG GAGATCCGGA TCGGAGACGC TTCAGTTCTG GAGATCGGAC 
GGTCCTGGAA GGTCTCCGAA GCTGAAAACG GTGGTTAGGT CGTCGTTTTC CGGTGTTCCG 
GAGAAGCCTC TCGGTCTCTA CGATCCTTCC TTCGATAAAG ACTCTTGTGG AGTCGGTTTC 
GTTGCGGAGT TATCCGGCGA GACCAGCCGC AAAACGGTGA CTGATTCATT GGAGATGTTG 
ATACGTATGA CTCATAGAGG CGCTTGTGGT TGTGAGAGCA ACACTGGTGA CGGCGCTGGG 
ATTCTCGTCG GTCTTCCTCA TGAGTTCTAC GCAGAGGCAA CGAAAGAGCT TGGCTTTGTG 
CTCCCTCCTG CTGGAAAGTA TGCTGTTGGT ATGTTCTTCT TGCCAAAAGC TGAGTCCCGG 
AGAAATGAGA GCAAAAATGT TTTCACCAAG GTTGCTGAGT CGCTTGGGCA TTCAGTTATT 
GGGTGGAGGC CAGTTCCTAC TGATAACTCT GGGCTGGGGA AGTCTGCTTT GCAGACCGAG 
CCGATTATCG AGCAGGTTTT CCTCACTCCA ACTACTAAAT CGAAAGCCGA CCTTGAGCAA 
CAGATGTATA TCCTGAGGAG GGTTTCCATG GTTGCTATCC GAGCAGCGTT GAACCTTGAA 
CATGGTGCGA TGAAGGATTT CTACATCTGT TCTTTGTCTT CTAGGACTGT TGTCTACAAA 
GGGCAACTGA AGCCTGATCA GCTCAAGGAC TATTACTACG CGGATCTTGG GAGTGAAAGG 
TTTACGAGCT ACATGGCCCT GGTCCACTCT CGATTCTCTA CCAACACATT TCCCAGCTGG 
GATCGTGCTC AGCCAATGCG GGTTTTGGGC CACAACGGGG AGATTAATAC GCTTAGAGGA 
AACGTGAACT GGATGAGGGC TCGTGAGGGC CTGCTTAAGT GCAAGGAACT TGGTTTATCA 
AAGAACGAGT TGAAGAAGCT TCTACCTATT GTGGATGTTA GCTCTTCTGA CTCAGGTGCT 
TTTGATGGTG TTCTTGAGCT TCTTGTCCGA GCTGGCAGAA GTCTTCCTGA AGCTGTTATG 
ATGATGATCC CAGAAGCATG GCAGAATGAC AAAAACATAG ATCCAAGCAG GAAAGCATTT 
TATGAATACT TATCTGCCCT AATGGAGCCA TGGGATGGAC CTGCTCTCAT CTCATTTACC 
GACGGTCGCT ACTTAGGTGC CACATTGGAC CGTAATGGAC TTCGTCCTGG TAGGTTCTAC 
ATTACCCACA GTGGAAGGGT TATCATGGCT AGTGAAGTTG GGGTAGTGGA TGTACCGCCT 
GAAGATGTGA TGAGGAAAGG AAGGCTTAAC CCGGGCATGA TGCTTCTTGT CGATTTTGAG 
AAGCATATTG TTGTTGATGA CGATGCCTTG AAGCAACAGT ATTCACAGGC AAGGCCGTAT 
GGTGAATGGC TTGAAAGGCA AAAGATTGAA CTTAAAGACA TCATTAAGTC AGTTCCGGAG 
AGTGAACGGA TTGCTTCTCC AATTTCTGGG GTTGTACCGG CGTCTGATGA TGATGACTCA 
ATGGAGAGCA TGGGCATTCA TGGTTTATTG TCTCCACTAA AAGCCTTTGG CTATACAGTT 
GAGGCCTTGG AAATGCTGCT GCTTCCGATG GCTAAAGATG GCACTGAAGC ACTTGGTTCT 
ATGGGAAATG ATACTCCTTT AGCTGTGATG TCAAACAGAG AGAAATTATG TTTTGAGTAC 
TTCAAGCAAA TGTTTGCTCA AGTAACCAAT CCTCCAATCG ATCCCATTCG TGAGAAAATT 
GTTACTTCAA TGGAGTGCAT GATCGGTCCA GAAGGTGATC TAACCGAAAC AACTGAAGAG 
CAATGTCACC GTCTCTCCCT AAAAGGTCCT CTTTTGACCA TAGAAGAGAT GGAATCAATC 
AAGAAAATGA ATTACAGAGG CTGGAGGACT AAAGTTCTTG ATATAACGTA TCAAAAAGAC 
CAAGGTACAA AAGGGTTGGA GAAGACATTG GACCGAATTT GTGATGAAGC AAATGAAGCG 
ATAAAGGAGG GCTACACATT ACTGGTTTTG TCTGATCGAG CTTTCTCCGC TACGCGAGTT 
GCTGTGAGCT CTCTCTTGGC TGTTGGTGCC GTCCATCACC ACCTGGTTAA GACACTTGCG 
CGCACTCAAG TTGGTTTGGT AGTGGAATCT GCTGAACCAC GTGAAGTGCA TCATTTCTGC 
ACACTTGTCG GATTCGGAGC AGATGCTATT TGCCCATATT TGGCTGTTGA GGCCGTTTTT 
AGATTGCAGG TCGATGGCAA GATACCACCC AAATCTAATG GGGAATTCCA TTCGAAAGAG 
GAGCTGGTCA AGAAGTATTA CAAGGCCAGT AACTATGGAA TGATGAAGGT TCTTGCCAAA 
ATGGGAATTT CTACTCTGGC CTCATACAAG GGTGCTCAAA TATTTGAAGC TCTTGGGCTT 
TCGTCTGAAG TAATAGAGAA GTGTTTTGCT GGCACCCCAA GCAGAGTGGA AGGAGCAACA 
TTTGAGATGC TTGCACGAGA TGCGCTTCTT TTGCATGAGA TGGCCTTTCC AGCCCGTGGC 
TATGCCCCTG GAAGTGCTGA AGCCTCGGCA CTTTCCAACC CTGGGAACTA TCACTGGAGG 
AAAAATGGGG AGATTCATCT CAATGATCCT CTGGCGATTG CAAAACTGCA AGAGGCAGCA 
CGGACTAACA GTGTCGCTGC CTACAAAGAA TACTCGAAGC GTATAAATGA ATTGAACAAG 
CAAAGTAACT TGCGCGGGTT TATGAAGTTC AAAGAAGCAG ATGTAAAGAT TCCTCTAGAT 
GAAGTGGAGC CTGCCAGTGA AATCGTTAAA CGGTTCTGCA CTGGCGCCAT GAGTTATGGA 
TCAATATCAC TGGAGGCGCA TACAACCCTT GCAATGGCCA TGAACAAACT TGGAGGAAAG 
TCCAATACAG GTGAGGGAGG GGAACTGCCA TCCCGTATGG AGCCTCTTCC AGATGGTTCA 
AGGAATCCGA AAAGAAGTTC CATCAAACAA ATTGCTAGTG GAAGATTTGG AGTTTCGAGC 
TACTATCTTA CTAATGCCGA TGAGCTGCAG ATAAAGATGG CTCAGGGTGC TAAACCTGGT 
GAAGGAGGCG AGCTACCTGG CCACAAGGTT ATAGGAGACA TCGCTATCAC CAGAAATTCC 
ACCGCCGGTG TGGGGCTTAT CAGTCCACCT CCTCATCATG ATATCTACTC TATCGAGGAT 
CTTGCCCAAC TAATTCATGA TCTTAAGAAT GCCAACCCTG GGGCCCGTAT TAGTGTTAAA 
CTTGTATCGG AAGCTGGTGT TGGAGTTATT GCTAGCGGTG TGGTGAAAGG CCACGCTGAC 
CATGTTCTGA TAGCAGGTCA TGATGGAGGT ACTGGTGCAT CTCGCTGGAC CGGTATAAAA 
AATGCTGGTC TTCCGTGGGA ACTTGGTCTA GCTGAGACAC ACCAAACTCT TGTCGCTAAT 
GACCTTCGTG GTCGTACTGT TCTTCAGACA GATGGCCAAC TGAAAACTGG CAGAGATGTT 
GCAGTTGCTG CGCTTCTTGG TGCAGAAGAG TTTGGTTTTA GTACTGCACC CCTTATTACA 
CTCGGGTGTA TCATGATGAG AAAATGCCAC AAGAACACCT GCCCTGTGGG AATTGCTACT 
CAAGATCCAG TTCTCCGTGA AAAATTTGCT GGGGAGCCTG AACATGTCAT TAATTTCTTC 
TTTATGCTTG CTGAGGAAGT TAGGGAGATC ATGTCTGGAC TTGGTTTCCG AACTCTAACT 
GAAATGATTG GTCGGGCAGA TATGCTTGAA CTTGACAGGG AAGTGGTCAA GAACAATGAT 
AAGCTGGAGA ACATTGACCT CTCTCTTTTG CTCAAACCCG CTGCTGAGAT TCGTCCTGGG 
GCTGCTCAGT ACTGTGTCCA GAAACAAGAT CATGGCTTAG ACATGGCTTT GGATCAAGAG 
CTTATTGCAT TGTCCAAGTC TGCTCTAGAG AAATCTCTTC CGGTGTACAT TGAAACTCCT 
ATCTGCAACG TCAACCGTGC TGTTGGGACT ATGCTGAGCC ATGAGGTTAC CAAACGCTAT 
CACTTGGCTG GGCTACCAAA GGATACAATT CATATCAAGT TCAAAGGAAG TGCTGGGCAG 
AGTCTTGGTG CTTTCCTGTG TCCTGGAATT ACGCTAGAAC TGGAGGGTGA TAGCAACGAC 
TATGTTGGAA AAGGTCTCTC CGGAGGAAAA GTCGTTGTCT ACCCTCCTAA AGGAAGCAGC 
TTTGATCCAA AAGAAAACAT TGTTATCGGT AACGTAGCAC TTTACGGGGC CACTAGTGGT 
GAAGCCTACT TTAATGGGAT GGCTGCTGAA AGATTCTCTG TTCGTAATTC TGGTGCCAAA 
GCTGTTGTGG AAGGTGTTGG TGATCATGGT TGTGAGTACA TGACTGGTGG TACTGTTGTG 
GTGCTTGGAA AGACTGGTAG AAACTTTGCT GCTGGTATGA GTGGTGGTAT TGCTTATGTC 
CTTGATGTGG ATGGAAAGTT TCACACGAGA TGCAACCTTG AACTGGTTGA TCTGGACAAA 
GTTGAAGATG AAGACGACAA AATGACGCTA AAAATGATGA TCCAGCAACA TCAGCGACAC 
ACAGACAGCC AACTTGCTCA AGAAGTTCTT GCGGACTTTG AGAATTTGGT ACCGAAGTTT 
GTCAAGGTTT TCCCAAGAGA TTACAAACGT GTTTTATCAG CCATGAAACA TGAAGAGGTC 
TCGAATCAAG CCATAGAGCG TGCTTCTGAA GAAGCTGACG AGATGGAAGA GAAAGAACTA 
GAGGAGAAAG ACGCCTTTAC AGAGCTGAAG AACATGGCAG CTGCCTCTTC AAAAGAGGAG 
ATGTCAGGAA ACGGCGTGGC AGCTGAAGCT CCGAAGAAAC CTTCTCTGGT AAATGATGCT 
GTTAAACATC GTGGTTTCAT TGCTTATGAA CGTGAGGGAG TTAAGTACAG GGACCCTAAT 
GTTCGTCTTC ATGACTGGAA CGAAGTCATG GAGGAATCAA AACCTGGACC GCTCCTTACA 
ACTCAGTCTG CTCGCTGCAT GGACTGTGGA ACTCCATTCT GCCACCAGGA GAACTCTGGG 
TGTCCGCTTG GTAACAAGAT CCCTGAATTC AATGAACTTG TCTACCAGAA CAGATGGCAA 
GAAGCGTTGA ACCGTCTACT TGAGACAAAT AACTTTCCCG AATTCACTGG GCGAGTTTGC 
CCTGCACCAT GTGAAGGTTC TTGTGTTCTT GGAATAATTG AGAACCCTGT TTCTATCAAA 
AGCATCGAAT GTGCTATCAT AGATAAAGCC TTTGATGAAG GGTGGATGGT ACCAAGACCT 
CCTCTCAAGA GAACAGGGAG AAAAGTTGCA ATTATCGGAA GTGGGCCGGC TGGACTGGCT 
GCTGCTGACC AGCTGAACAA AATGGGTCAC TCGGTAACCG TGTATGAGCG TGCTGACAGA 
ATTGGCGGGC TTATGATGTA TGGAGTCCCA AACATGAAGA CTGACAAAAT TGACATAGTT 
CAACGTCGGG TTGATCTTAT GACCAAAGAA GGTATCAACT TTGTGGTCAA TGCCAATATT 
GGAAAAGACC CGTCTTTCTC CCTGGATGGA CTTAAGGAGG AGAATGATGC AATCGTTCTT 
GCTGTTGGTT CCACAAAACC AAGAGATCTT CCAGTGTCTG GTCGTGAGCT ATCTGGTGTT 
CACTTTGCCA TGGAGTTTCT TCATGCAAAC ACCAAAAGCT TGCTTGATAG CAATCTTGAA 
GATGGCAATT ACATTTCCGC AAAGGGGAAA AAGGTTGTCG TTATTGGTGG AGGTGATACG 
GGCACAGATT GCATTGGAAC ATCTATCCGC CATGGATGCA CCAACATTGT AAACCTAGAG 
CTTCTTCCTC AGCCACCTTC AACGAGAGCT CCAGGAAACC CTTGGCCACA GTGGCCTCGT 
GTATTCCGTA TTGACTACGG ACATCAAGAA GCAGCGACCA AATTCGGAAA GGACCCTAGA 
ACCTATGAGG TCTTAACTAA GCGATTCATA GGAGATGACA ATGGAAACGT GAAAGGGCTA 
GAGATTGTGC GCGTGAGTTG GGAGAAGGAT GAAACCGGGA GGTTCCAATT CAAGGAGATC 
GAAGGCTCCG AGGAAATAAT TGAAGCGGAC CTTGTTTTCT TAGCCATGGG ATTCCTTGGA 
CCTGAGCCAA CACTTGCCGA GAAACTAGGA CTCGAATGTG ACAACAGGTC GAACTTTAAG 
GCAGAGTATG GCCGGTTTTC AACAACAGTG GAAGGTGTTT TTGCTGCAGG TGATTGTCGA 
AGAGGACAGT CTTTGGTGGT CTGGGCCATC TCAGAAGGAC GCCAAGCTGC ATCTCAGGTC 
GACAAATACC TCAGCAAAAC TGATGACGAC CAAGCAGATG CCAAGCTACG ACAGAGCCTC 
AACCAGATGA AGCACAACGA CACTAGGACG AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024011475.1
CDS	1..6636
Translation	MSAASSSSVLHLRNNQLLPIRSLKNPTVVASQLAVAPGVGRRRACTARCSVKKPAAPESPFLGTRMRRSGSETLQFWRSDGPGRSPKLKTVVRSSFSGVPEKPLGLYDPSFDKDSCGVGFVAELSGETSRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHEFYAEATKELGFVLPPAGKYAVGMFFLPKAESRRNESKNVFTKVAESLGHSVIGWRPVPTDNSGLGKSALQTEPIIEQVFLTPTTKSKADLEQQMYILRRVSMVAIRAALNLEHGAMKDFYICSLSSRTVVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKNELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKAFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSQARPYGEWLERQKIELKDIIKSVPESERIASPISGVVPASDDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGTEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLTIEEMESIKKMNYRGWRTKVLDITYQKDQGTKGLEKTLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLLAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVFRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLARDALLLHEMAFPARGYAPGSAEASALSNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGFMKFKEADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLPDGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTLTEMIGRADMLELDREVVKNNDKLENIDLSLLLKPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPKDTIHIKFKGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFHTRCNLELVDLDKVEDEDDKMTLKMMIQQHQRHTDSQLAQEVLADFENLVPKFVKVFPRDYKRVLSAMKHEEVSNQAIERASEEADEMEEKELEEKDAFTELKNMAAASSKEEMSGNGVAAEAPKKPSLVNDAVKHRGFIAYEREGVKYRDPNVRLHDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFDEGWMVPRPPLKRTGRKVAIIGSGPAGLAAADQLNKMGHSVTVYERADRIGGLMMYGVPNMKTDKIDIVQRRVDLMTKEGINFVVNANIGKDPSFSLDGLKEENDAIVLAVGSTKPRDLPVSGRELSGVHFAMEFLHANTKSLLDSNLEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEAATKFGKDPRTYEVLTKRFIGDDNGNVKGLEIVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAASQVDKYLSKTDDDQADAKLRQSLNQMKHNDTRTS

Target ORF information:

RefSeq Version	XM_024155707.1
Organism	Eutrema salsugineum
Definition	Eutrema salsugineum glutamate synthase 1 [NADH], chloroplastic (LOC18016490), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024155707.1

ORF Insert Sequence:

ATGTCGGCAG CTTCTTCAAG CTCCGTTCTC CACCTCCGAA ACAATCAGTT GTTACCAATC 
CGTTCTCTGA AAAATCCCAC CGTCGTTGCT TCTCAATTGG CCGTCGCGCC TGGCGTCGGT 
CGGAGACGAG CATGTACCGC ACGATGCTCC GTGAAGAAAC CGGCGGCTCC CGAGAGTCCT 
TTCCTGGGTA CTCGGATGAG GAGATCCGGA TCGGAGACGC TTCAGTTCTG GAGATCGGAC 
GGTCCTGGAA GGTCTCCGAA GCTGAAAACG GTGGTTAGGT CGTCGTTTTC CGGTGTTCCG 
GAGAAGCCTC TCGGTCTCTA CGATCCTTCC TTCGATAAAG ACTCTTGTGG AGTCGGTTTC 
GTTGCGGAGT TATCCGGCGA GACCAGCCGC AAAACGGTGA CTGATTCATT GGAGATGTTG 
ATACGTATGA CTCATAGAGG CGCTTGTGGT TGTGAGAGCA ACACTGGTGA CGGCGCTGGG 
ATTCTCGTCG GTCTTCCTCA TGAGTTCTAC GCAGAGGCAA CGAAAGAGCT TGGCTTTGTG 
CTCCCTCCTG CTGGAAAGTA TGCTGTTGGT ATGTTCTTCT TGCCAAAAGC TGAGTCCCGG 
AGAAATGAGA GCAAAAATGT TTTCACCAAG GTTGCTGAGT CGCTTGGGCA TTCAGTTATT 
GGGTGGAGGC CAGTTCCTAC TGATAACTCT GGGCTGGGGA AGTCTGCTTT GCAGACCGAG 
CCGATTATCG AGCAGGTTTT CCTCACTCCA ACTACTAAAT CGAAAGCCGA CCTTGAGCAA 
CAGATGTATA TCCTGAGGAG GGTTTCCATG GTTGCTATCC GAGCAGCGTT GAACCTTGAA 
CATGGTGCGA TGAAGGATTT CTACATCTGT TCTTTGTCTT CTAGGACTGT TGTCTACAAA 
GGGCAACTGA AGCCTGATCA GCTCAAGGAC TATTACTACG CGGATCTTGG GAGTGAAAGG 
TTTACGAGCT ACATGGCCCT GGTCCACTCT CGATTCTCTA CCAACACATT TCCCAGCTGG 
GATCGTGCTC AGCCAATGCG GGTTTTGGGC CACAACGGGG AGATTAATAC GCTTAGAGGA 
AACGTGAACT GGATGAGGGC TCGTGAGGGC CTGCTTAAGT GCAAGGAACT TGGTTTATCA 
AAGAACGAGT TGAAGAAGCT TCTACCTATT GTGGATGTTA GCTCTTCTGA CTCAGGTGCT 
TTTGATGGTG TTCTTGAGCT TCTTGTCCGA GCTGGCAGAA GTCTTCCTGA AGCTGTTATG 
ATGATGATCC CAGAAGCATG GCAGAATGAC AAAAACATAG ATCCAAGCAG GAAAGCATTT 
TATGAATACT TATCTGCCCT AATGGAGCCA TGGGATGGAC CTGCTCTCAT CTCATTTACC 
GACGGTCGCT ACTTAGGTGC CACATTGGAC CGTAATGGAC TTCGTCCTGG TAGGTTCTAC 
ATTACCCACA GTGGAAGGGT TATCATGGCT AGTGAAGTTG GGGTAGTGGA TGTACCGCCT 
GAAGATGTGA TGAGGAAAGG AAGGCTTAAC CCGGGCATGA TGCTTCTTGT CGATTTTGAG 
AAGCATATTG TTGTTGATGA CGATGCCTTG AAGCAACAGT ATTCACAGGC AAGGCCGTAT 
GGTGAATGGC TTGAAAGGCA AAAGATTGAA CTTAAAGACA TCATTAAGTC AGTTCCGGAG 
AGTGAACGGA TTGCTTCTCC AATTTCTGGG GTTGTACCGG CGTCTGATGA TGATGACTCA 
ATGGAGAGCA TGGGCATTCA TGGTTTATTG TCTCCACTAA AAGCCTTTGG CTATACAGTT 
GAGGCCTTGG AAATGCTGCT GCTTCCGATG GCTAAAGATG GCACTGAAGC ACTTGGTTCT 
ATGGGAAATG ATACTCCTTT AGCTGTGATG TCAAACAGAG AGAAATTATG TTTTGAGTAC 
TTCAAGCAAA TGTTTGCTCA AGTAACCAAT CCTCCAATCG ATCCCATTCG TGAGAAAATT 
GTTACTTCAA TGGAGTGCAT GATCGGTCCA GAAGGTGATC TAACCGAAAC AACTGAAGAG 
CAATGTCACC GTCTCTCCCT AAAAGGTCCT CTTTTGACCA TAGAAGAGAT GGAATCAATC 
AAGAAAATGA ATTACAGAGG CTGGAGGACT AAAGTTCTTG ATATAACGTA TCAAAAAGAC 
CAAGGTACAA AAGGGTTGGA GAAGACATTG GACCGAATTT GTGATGAAGC AAATGAAGCG 
ATAAAGGAGG GCTACACATT ACTGGTTTTG TCTGATCGAG CTTTCTCCGC TACGCGAGTT 
GCTGTGAGCT CTCTCTTGGC TGTTGGTGCC GTCCATCACC ACCTGGTTAA GACACTTGCG 
CGCACTCAAG TTGGTTTGGT AGTGGAATCT GCTGAACCAC GTGAAGTGCA TCATTTCTGC 
ACACTTGTCG GATTCGGAGC AGATGCTATT TGCCCATATT TGGCTGTTGA GGCCGTTTTT 
AGATTGCAGG TCGATGGCAA GATACCACCC AAATCTAATG GGGAATTCCA TTCGAAAGAG 
GAGCTGGTCA AGAAGTATTA CAAGGCCAGT AACTATGGAA TGATGAAGGT TCTTGCCAAA 
ATGGGAATTT CTACTCTGGC CTCATACAAG GGTGCTCAAA TATTTGAAGC TCTTGGGCTT 
TCGTCTGAAG TAATAGAGAA GTGTTTTGCT GGCACCCCAA GCAGAGTGGA AGGAGCAACA 
TTTGAGATGC TTGCACGAGA TGCGCTTCTT TTGCATGAGA TGGCCTTTCC AGCCCGTGGC 
TATGCCCCTG GAAGTGCTGA AGCCTCGGCA CTTTCCAACC CTGGGAACTA TCACTGGAGG 
AAAAATGGGG AGATTCATCT CAATGATCCT CTGGCGATTG CAAAACTGCA AGAGGCAGCA 
CGGACTAACA GTGTCGCTGC CTACAAAGAA TACTCGAAGC GTATAAATGA ATTGAACAAG 
CAAAGTAACT TGCGCGGGTT TATGAAGTTC AAAGAAGCAG ATGTAAAGAT TCCTCTAGAT 
GAAGTGGAGC CTGCCAGTGA AATCGTTAAA CGGTTCTGCA CTGGCGCCAT GAGTTATGGA 
TCAATATCAC TGGAGGCGCA TACAACCCTT GCAATGGCCA TGAACAAACT TGGAGGAAAG 
TCCAATACAG GTGAGGGAGG GGAACTGCCA TCCCGTATGG AGCCTCTTCC AGATGGTTCA 
AGGAATCCGA AAAGAAGTTC CATCAAACAA ATTGCTAGTG GAAGATTTGG AGTTTCGAGC 
TACTATCTTA CTAATGCCGA TGAGCTGCAG ATAAAGATGG CTCAGGGTGC TAAACCTGGT 
GAAGGAGGCG AGCTACCTGG CCACAAGGTT ATAGGAGACA TCGCTATCAC CAGAAATTCC 
ACCGCCGGTG TGGGGCTTAT CAGTCCACCT CCTCATCATG ATATCTACTC TATCGAGGAT 
CTTGCCCAAC TAATTCATGA TCTTAAGAAT GCCAACCCTG GGGCCCGTAT TAGTGTTAAA 
CTTGTATCGG AAGCTGGTGT TGGAGTTATT GCTAGCGGTG TGGTGAAAGG CCACGCTGAC 
CATGTTCTGA TAGCAGGTCA TGATGGAGGT ACTGGTGCAT CTCGCTGGAC CGGTATAAAA 
AATGCTGGTC TTCCGTGGGA ACTTGGTCTA GCTGAGACAC ACCAAACTCT TGTCGCTAAT 
GACCTTCGTG GTCGTACTGT TCTTCAGACA GATGGCCAAC TGAAAACTGG CAGAGATGTT 
GCAGTTGCTG CGCTTCTTGG TGCAGAAGAG TTTGGTTTTA GTACTGCACC CCTTATTACA 
CTCGGGTGTA TCATGATGAG AAAATGCCAC AAGAACACCT GCCCTGTGGG AATTGCTACT 
CAAGATCCAG TTCTCCGTGA AAAATTTGCT GGGGAGCCTG AACATGTCAT TAATTTCTTC 
TTTATGCTTG CTGAGGAAGT TAGGGAGATC ATGTCTGGAC TTGGTTTCCG AACTCTAACT 
GAAATGATTG GTCGGGCAGA TATGCTTGAA CTTGACAGGG AAGTGGTCAA GAACAATGAT 
AAGCTGGAGA ACATTGACCT CTCTCTTTTG CTCAAACCCG CTGCTGAGAT TCGTCCTGGG 
GCTGCTCAGT ACTGTGTCCA GAAACAAGAT CATGGCTTAG ACATGGCTTT GGATCAAGAG 
CTTATTGCAT TGTCCAAGTC TGCTCTAGAG AAATCTCTTC CGGTGTACAT TGAAACTCCT 
ATCTGCAACG TCAACCGTGC TGTTGGGACT ATGCTGAGCC ATGAGGTTAC CAAACGCTAT 
CACTTGGCTG GGCTACCAAA GGATACAATT CATATCAAGT TCAAAGGAAG TGCTGGGCAG 
AGTCTTGGTG CTTTCCTGTG TCCTGGAATT ACGCTAGAAC TGGAGGGTGA TAGCAACGAC 
TATGTTGGAA AAGGTCTCTC CGGAGGAAAA GTCGTTGTCT ACCCTCCTAA AGGAAGCAGC 
TTTGATCCAA AAGAAAACAT TGTTATCGGT AACGTAGCAC TTTACGGGGC CACTAGTGGT 
GAAGCCTACT TTAATGGGAT GGCTGCTGAA AGATTCTCTG TTCGTAATTC TGGTGCCAAA 
GCTGTTGTGG AAGGTGTTGG TGATCATGGT TGTGAGTACA TGACTGGTGG TACTGTTGTG 
GTGCTTGGAA AGACTGGTAG AAACTTTGCT GCTGGTATGA GTGGTGGTAT TGCTTATGTC 
CTTGATGTGG ATGGAAAGTT TCACACGAGA TGCAACCTTG AACTGGTTGA TCTGGACAAA 
GTTGAAGATG AAGACGACAA AATGACGCTA AAAATGATGA TCCAGCAACA TCAGCGACAC 
ACAGACAGCC AACTTGCTCA AGAAGTTCTT GCGGACTTTG AGAATTTGGT ACCGAAGTTT 
GTCAAGGTTT TCCCAAGAGA TTACAAACGT GTTTTATCAG CCATGAAACA TGAAGAGGTC 
TCGAATCAAG CCATAGAGCG TGCTTCTGAA GAAGCTGACG AGATGGAAGA GAAAGAACTA 
GAGGAGAAAG ACGCCTTTAC AGAGCTGAAG AACATGGCAG CTGCCTCTTC AAAAGAGGAG 
ATGTCAGGAA ACGGCGTGGC AGCTGAAGCT CCGAAGAAAC CTTCTCTGGT AAATGATGCT 
GTTAAACATC GTGGTTTCAT TGCTTATGAA CGTGAGGGAG TTAAGTACAG GGACCCTAAT 
GTTCGTCTTC ATGACTGGAA CGAAGTCATG GAGGAATCAA AACCTGGACC GCTCCTTACA 
ACTCAGTCTG CTCGCTGCAT GGACTGTGGA ACTCCATTCT GCCACCAGGA GAACTCTGGG 
TGTCCGCTTG GTAACAAGAT CCCTGAATTC AATGAACTTG TCTACCAGAA CAGATGGCAA 
GAAGCGTTGA ACCGTCTACT TGAGACAAAT AACTTTCCCG AATTCACTGG GCGAGTTTGC 
CCTGCACCAT GTGAAGGTTC TTGTGTTCTT GGAATAATTG AGAACCCTGT TTCTATCAAA 
AGCATCGAAT GTGCTATCAT AGATAAAGCC TTTGATGAAG GGTGGATGGT ACCAAGACCT 
CCTCTCAAGA GAACAGGGAG AAAAGTTGCA ATTATCGGAA GTGGGCCGGC TGGACTGGCT 
GCTGCTGACC AGCTGAACAA AATGGGTCAC TCGGTAACCG TGTATGAGCG TGCTGACAGA 
ATTGGCGGGC TTATGATGTA TGGAGTCCCA AACATGAAGA CTGACAAAAT TGACATAGTT 
CAACGTCGGG TTGATCTTAT GACCAAAGAA GGTATCAACT TTGTGGTCAA TGCCAATATT 
GGAAAAGACC CGTCTTTCTC CCTGGATGGA CTTAAGGAGG AGAATGATGC AATCGTTCTT 
GCTGTTGGTT CCACAAAACC AAGAGATCTT CCAGTGTCTG GTCGTGAGCT ATCTGGTGTT 
CACTTTGCCA TGGAGTTTCT TCATGCAAAC ACCAAAAGCT TGCTTGATAG CAATCTTGAA 
GATGGCAATT ACATTTCCGC AAAGGGGAAA AAGGTTGTCG TTATTGGTGG AGGTGATACG 
GGCACAGATT GCATTGGAAC ATCTATCCGC CATGGATGCA CCAACATTGT AAACCTAGAG 
CTTCTTCCTC AGCCACCTTC AACGAGAGCT CCAGGAAACC CTTGGCCACA GTGGCCTCGT 
GTATTCCGTA TTGACTACGG ACATCAAGAA GCAGCGACCA AATTCGGAAA GGACCCTAGA 
ACCTATGAGG TCTTAACTAA GCGATTCATA GGAGATGACA ATGGAAACGT GAAAGGGCTA 
GAGATTGTGC GCGTGAGTTG GGAGAAGGAT GAAACCGGGA GGTTCCAATT CAAGGAGATC 
GAAGGCTCCG AGGAAATAAT TGAAGCGGAC CTTGTTTTCT TAGCCATGGG ATTCCTTGGA 
CCTGAGCCAA CACTTGCCGA GAAACTAGGA CTCGAATGTG ACAACAGGTC GAACTTTAAG 
GCAGAGTATG GCCGGTTTTC AACAACAGTG GAAGGTGTTT TTGCTGCAGG TGATTGTCGA 
AGAGGACAGT CTTTGGTGGT CTGGGCCATC TCAGAAGGAC GCCAAGCTGC ATCTCAGGTC 
GACAAATACC TCAGCAAAAC TGATGACGAC CAAGCAGATG CCAAGCTACG ACAGAGCCTC 
AACCAGATGA AGCACAACGA CACTAGGACG AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Reference Genome of the Halophytic Plant Eutrema salsugineum.
Frontiers in plant science446(2013)
Yang R,Jarvis DE,Chen H,Beilstein MA,Grimwood J,Jenkins J,Shu S,Prochnik S,Xin M,Ma C,Schmutz J,Wing RA,Mitchell-Olds T,Schumaker KS,Wang X

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オンライン注文

LOC18016490 cDNA ORF clone, Eutrema salsugineum

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

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