NRDE2 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRDE2
Entrez Gene ID	100062646
Full Name	NRDE-2, necessary for RNA interference, domain containing
General protein information	Preferred Names NRDE-2, necessary for RNA interference, domain containing Names protein NRDE2 homolog
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005605398.2	XP_005605455.1	protein NRDE2 homolog
XM_005605398.3	XP_005605455.2	protein NRDE2 homolog

Pathways from BioSystems
Gene Ontology

The following NRDE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRDE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb58572	XM_005605398.3 Latest version!	Equus caballus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb14026	XM_005605398.2 Latest version!	Equus caballus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRDE2 ( XM_005605398.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005605455.2 Equus caballus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Price & Availability↑

CloneID	OHb58572
Clone ID Related Accession (Same CDS sequence)	XM_005605398.3
Accession Version	XM_005605398.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	protein NRDE2 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009167.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005605398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCCCGGCCTT TGCGGGCGTT AGTGAAGCTC CCGCTAGCGG AAGTTCTAGG 
AAAGAATTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GAAGCATACC ATCTCTGAGC 
CAACAAACTG AAGAGACCAC AGCTCTTGCT TCTGAAGGGT TGCCACTGAC AAGGAGTCCT 
CTGAAATCAG AGCCATCAGA GGAAAGTGAC ACTAACAGAA AGCTCAAATC TATAAGCAGA 
AAAAAGAAGA AAGAGAAAAA GAAGAAAAGG AAGCATCAGC ATCATAAGAA AACCAAGAGA 
AAACATGGGC AGTCGAGTAG CAGTGGATCT GAGCCAGATA CTGATTCTGA AAAGGACAGA 
TCTTACAGAA GCATCAGAGA CAGTAAAAGG GAATCAGAGA AACCAAATCA GCAAAATAAT 
GCCACTGCTG ATAGTGGACA TCGCCTTGTT TGGCTTGAGG ACGTTCAGGC TCTGACAGGA 
GAAACCTTCA GAACAGATAA GAAGCCGGAT CCTGCAAACT GGGAGTATAA GTCTCTCTAC 
CGTGGAGACA TAGCAAGGTA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCAAAG 
AAGCAGTGTA TATCTTGGGA GGGGACTTCC ACGGGAAAGA AGCATCCACA CAAGCGTGTT 
GAACGCTATT TTATGAAGAA GAACGTGGGA TTAATGAACA TTGACGGAGT TGCCGTTAGC 
AGTCAAACTG CCCCTCCCTC GTCTGAGCCC GTCTCGTTTA TCCCAGTGAA GGACTCAGAT 
GACGCGGCTC CTCCTGTTAC AGCCTGGTTG CACCCTCTGG GGATTTATGA TCAGTCCACC 
ACACAGTGGT TACAAGGAAA GGGTGCTTCA GAGCAAGAAT CAAAGCAGCC AGACTCACAG 
GTGGACAGAG AGAGCGCGCT GCTCAAGGCC AAGGTGGAGG AGTTTAACAG GAGGGTCCGG 
GAGCATCCTC GGGATGTTCA GCTGTGGATG GCGTTTGTTG CTTTTCAGGA CGAGGTCATG 
AAAAGTCCGG GCCTGTATGC CATCGAGGAA GGAGAGCAGG AAAAGCGAAA GAGGTCCCTG 
AAGCTGCTTC TGGAGAAGAA GCTGGCCATC CTGGAGCGGG CCATTGAAAG CAACCCGAGC 
TGCGTGGACC TGAAGCTCGC CAAGCTGCAG CTCTGCACGG AGCTCTGGGA GCCCGCCACC 
CTGCTCAGGG AGTGGCAGAA ACTCATATTC TTACATCCCA ACAATACAGC CCTTTGGCAT 
AAATATCTTT TATTTTGCCA GAGCCAGTTT AGCACCTTTT CAATATCAAA AATTCACAGT 
CTTTATGGAA AGTGCTTGAG TACCCTGTCT GCTGTTAAGG ATGGCAGCAT CTTGTCTCAC 
CCTGAGTTGC CCGGCACCGA GGAGGCCATG TTTGCCCTCT TTCTTCAGCA GTGCCACTTT 
CTGCGGCAGG CCGGTCACTC TGAGAAGGCC GTCTCTCTGT TCCAGGCCAT GGTCGACTTC 
ACCTTCTTCA AACCGGACAG TGTGAAAGGG CTGCCTACCA AAGGCCAGGT GGAGTTCTTT 
GAGCCCTTTT GGGACAGTGG AGAGCCCCGG GCTGGGGAGA AGGGCGCCCG GGGCTGGAGA 
GCGTGGATGC ACCAGCAGGA GCGGGGTGGC TGGGTGGTCA TCAGTCCAGA TGATGAGGAG 
GAAGAACCAG AAGATGATGA TCAGGAAATA AGAGATAAGA CTCTGCCCAG GTGGCAGATC 
TGGCTTGCTG CTGAGCGGTC CCGAGACCAG AGGCACTGGC GGCCGTGGCG CCCAGATAAG 
ACCAAGAAGC AGACTGAGGA AGACTGTGAG GATCCAGAGA GACAGGTGTT GTTTGATGAT 
ATTGGACAAT CTCTGATCCG ACTTTCCAGC CAGGATCTTC AGTTTCGGCT GATTGCGGCC 
TTCCTGCAGT TCTTGGGCGT GCCTTGTGGC GTCAGCCCTC CCGCCTCCTG CCTTTATCTG 
GCCATGGACG AGAACAGCAT CTTTGATAAC GGACTTTGTG ATGAAAAGCC GTTGACTTTC 
CTCAACCTGT CATTTTCTGG CGTCAGCTGT GTTGGCCGCA TGGACCAGTT GGGCTGCCGG 
CGCTGGACCA GAGCTCACGG TCGAGAGGGC GAGGACTTCA TCCGCAACGT CTTCCACCTG 
GTGCTGCCTT TGTTTTCCGG CAAGGAGAGA TCTCAGCTCT GCCTCTCCTG GTTACAGTAC 
GAGATTGCAA AGGTCATCTG GTGTCTGCAC ACTAAAAACA AGAAGAGATT AAAGTCACAA 
GGAAAGAACT GCAAAAAGCT AGCCAAGAAT CTCCTTAAGG AGCCGGAAAA CCGCAACGAT 
TTTTGCCTCT GGAAGCAGTA TGCGCATCTG GAGTGGTTGC TTGGCAACAT GGAGGACGCC 
AGGAAGGTTT TCGATACAGC ACTCAGCATG GCAGGGAGCA GAGAACTGAA GGATCACGAA 
CTCTGTGAGC TCAGTCTGCT CTACGCTGAG CTGGAGGTGG AGCTGTTGCT AGACTCGAGA 
GGGGCCGCCA CAGCCCGAGC TGTTCACATC TTAACCAGGC TGACTGAGAA CGGGCCCTGT 
GGGCCCTACG CCGGGCAGGT CTTGGCCGTT CACATTTTGA AAGCTCGGAA GGCTTATGAA 
CACGCACTGC AGGACTGCTT GGGTGAGGGC TGTGTCTCTG GACCGGCTCC CACCAACTCC 
TTGAACTGCC TAACTAGCTT GGTCAAATGC TTTATGCTCT TTCAGTATTT GACCGTGGGG 
ATCGATGCCG CTGTGCGGAT ATACGAGCAA GTATTTGCAA AACTGAAGGG CTCTGGTTCC 
CCAGAATGCT GTGGCCAGGA TGACAGTGCC GGCACCCAGC GTCTGAGCAG TGTTCTCGAG 
GCTGTCACAC TGATGCACAC CAGCCTGCTC AGATTCCACA TGAAAGTCAG CGTTTACCCG 
CTGGCCCCTC TGCGAGAGGC ACTCTCCGAG GCTTTAAAGT TGTATCCGGG CAACCAGGTT 
CTTTGGAGGT CATATGTACA GATTCAGAAT AAGTCTCACA GTGCCAGTAA GACCAGAAGA 
TTCTTTGATG CAGTCACCAG GTCTGCTAAA CCCTTAGAGC CTTGGTTGTT TGCCATTGAA 
GCCGAGAAAA TGAGGAAGAG ACTAGTGGAA ACTGTTCAGA GGGTAGATGG TAGAGAGGTC 
CACGCCACAA TTCCTGAGAC CGGCTTGACA CATCGGATCA AAGCCCTGTT TGAAAATGCA 
ATGCGGAGTG ACAGCGGCAG CCAGTGCCCC TTACTGTGGA GGATGTATTT GAATTTTTTG 
GTTTCCTTAG GAAACAAGGA AAGAAGCAAA GGTGTGTTCT ACAAAGCACT TCAGAATTGT 
CCTTGGGCAA AGGTGTTGTA CATGGACGCT GTGGAGTACT TCCCCGATGA GATGCAGGAG 
ATCCTGGACC TGATGACTGA GAAGGAGCTC CGGGTGCGCC TGCCGGTGGA GGAGCTGGAG 
CTTCTGCTTG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005605455.2
CDS	92..3589
Translation	MALFPAFAGVSEAPASGSSRKELDWLSNPSFCVGSIPSLSQQTEETTALASEGLPLTRSPLKSEPSEESDTNRKLKSISRKKKKEKKKKRKHQHHKKTKRKHGQSSSSGSEPDTDSEKDRSYRSIRDSKRESEKPNQQNNATADSGHRLVWLEDVQALTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTGKKHPHKRVERYFMKKNVGLMNIDGVAVSSQTAPPSSEPVSFIPVKDSDDAAPPVTAWLHPLGIYDQSTTQWLQGKGASEQESKQPDSQVDRESALLKAKVEEFNRRVREHPRDVQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLLLEKKLAILERAIESNPSCVDLKLAKLQLCTELWEPATLLREWQKLIFLHPNNTALWHKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPELPGTEEAMFALFLQQCHFLRQAGHSEKAVSLFQAMVDFTFFKPDSVKGLPTKGQVEFFEPFWDSGEPRAGEKGARGWRAWMHQQERGGWVVISPDDEEEEPEDDDQEIRDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSQDLQFRLIAAFLQFLGVPCGVSPPASCLYLAMDENSIFDNGLCDEKPLTFLNLSFSGVSCVGRMDQLGCRRWTRAHGREGEDFIRNVFHLVLPLFSGKERSQLCLSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENRNDFCLWKQYAHLEWLLGNMEDARKVFDTALSMAGSRELKDHELCELSLLYAELEVELLLDSRGAATARAVHILTRLTENGPCGPYAGQVLAVHILKARKAYEHALQDCLGEGCVSGPAPTNSLNCLTSLVKCFMLFQYLTVGIDAAVRIYEQVFAKLKGSGSPECCGQDDSAGTQRLSSVLEAVTLMHTSLLRFHMKVSVYPLAPLREALSEALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDAVTRSAKPLEPWLFAIEAEKMRKRLVETVQRVDGREVHATIPETGLTHRIKALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYMDAVEYFPDEMQEILDLMTEKELRVRLPVEELELLLED

Target ORF information:

RefSeq Version	XM_005605398.3
Organism	Equus caballus(horse)
Definition	Equus caballus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005605398.3

ORF Insert Sequence:

ATGGCGCTGT TCCCGGCCTT TGCGGGCGTT AGTGAAGCTC CCGCTAGCGG AAGTTCTAGG 
AAAGAATTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GAAGCATACC ATCTCTGAGC 
CAACAAACTG AAGAGACCAC AGCTCTTGCT TCTGAAGGGT TGCCACTGAC AAGGAGTCCT 
CTGAAATCAG AGCCATCAGA GGAAAGTGAC ACTAACAGAA AGCTCAAATC TATAAGCAGA 
AAAAAGAAGA AAGAGAAAAA GAAGAAAAGG AAGCATCAGC ATCATAAGAA AACCAAGAGA 
AAACATGGGC AGTCGAGTAG CAGTGGATCT GAGCCAGATA CTGATTCTGA AAAGGACAGA 
TCTTACAGAA GCATCAGAGA CAGTAAAAGG GAATCAGAGA AACCAAATCA GCAAAATAAT 
GCCACTGCTG ATAGTGGACA TCGCCTTGTT TGGCTTGAGG ACGTTCAGGC TCTGACAGGA 
GAAACCTTCA GAACAGATAA GAAGCCGGAT CCTGCAAACT GGGAGTATAA GTCTCTCTAC 
CGTGGAGACA TAGCAAGGTA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCAAAG 
AAGCAGTGTA TATCTTGGGA GGGGACTTCC ACGGGAAAGA AGCATCCACA CAAGCGTGTT 
GAACGCTATT TTATGAAGAA GAACGTGGGA TTAATGAACA TTGACGGAGT TGCCGTTAGC 
AGTCAAACTG CCCCTCCCTC GTCTGAGCCC GTCTCGTTTA TCCCAGTGAA GGACTCAGAT 
GACGCGGCTC CTCCTGTTAC AGCCTGGTTG CACCCTCTGG GGATTTATGA TCAGTCCACC 
ACACAGTGGT TACAAGGAAA GGGTGCTTCA GAGCAAGAAT CAAAGCAGCC AGACTCACAG 
GTGGACAGAG AGAGCGCGCT GCTCAAGGCC AAGGTGGAGG AGTTTAACAG GAGGGTCCGG 
GAGCATCCTC GGGATGTTCA GCTGTGGATG GCGTTTGTTG CTTTTCAGGA CGAGGTCATG 
AAAAGTCCGG GCCTGTATGC CATCGAGGAA GGAGAGCAGG AAAAGCGAAA GAGGTCCCTG 
AAGCTGCTTC TGGAGAAGAA GCTGGCCATC CTGGAGCGGG CCATTGAAAG CAACCCGAGC 
TGCGTGGACC TGAAGCTCGC CAAGCTGCAG CTCTGCACGG AGCTCTGGGA GCCCGCCACC 
CTGCTCAGGG AGTGGCAGAA ACTCATATTC TTACATCCCA ACAATACAGC CCTTTGGCAT 
AAATATCTTT TATTTTGCCA GAGCCAGTTT AGCACCTTTT CAATATCAAA AATTCACAGT 
CTTTATGGAA AGTGCTTGAG TACCCTGTCT GCTGTTAAGG ATGGCAGCAT CTTGTCTCAC 
CCTGAGTTGC CCGGCACCGA GGAGGCCATG TTTGCCCTCT TTCTTCAGCA GTGCCACTTT 
CTGCGGCAGG CCGGTCACTC TGAGAAGGCC GTCTCTCTGT TCCAGGCCAT GGTCGACTTC 
ACCTTCTTCA AACCGGACAG TGTGAAAGGG CTGCCTACCA AAGGCCAGGT GGAGTTCTTT 
GAGCCCTTTT GGGACAGTGG AGAGCCCCGG GCTGGGGAGA AGGGCGCCCG GGGCTGGAGA 
GCGTGGATGC ACCAGCAGGA GCGGGGTGGC TGGGTGGTCA TCAGTCCAGA TGATGAGGAG 
GAAGAACCAG AAGATGATGA TCAGGAAATA AGAGATAAGA CTCTGCCCAG GTGGCAGATC 
TGGCTTGCTG CTGAGCGGTC CCGAGACCAG AGGCACTGGC GGCCGTGGCG CCCAGATAAG 
ACCAAGAAGC AGACTGAGGA AGACTGTGAG GATCCAGAGA GACAGGTGTT GTTTGATGAT 
ATTGGACAAT CTCTGATCCG ACTTTCCAGC CAGGATCTTC AGTTTCGGCT GATTGCGGCC 
TTCCTGCAGT TCTTGGGCGT GCCTTGTGGC GTCAGCCCTC CCGCCTCCTG CCTTTATCTG 
GCCATGGACG AGAACAGCAT CTTTGATAAC GGACTTTGTG ATGAAAAGCC GTTGACTTTC 
CTCAACCTGT CATTTTCTGG CGTCAGCTGT GTTGGCCGCA TGGACCAGTT GGGCTGCCGG 
CGCTGGACCA GAGCTCACGG TCGAGAGGGC GAGGACTTCA TCCGCAACGT CTTCCACCTG 
GTGCTGCCTT TGTTTTCCGG CAAGGAGAGA TCTCAGCTCT GCCTCTCCTG GTTACAGTAC 
GAGATTGCAA AGGTCATCTG GTGTCTGCAC ACTAAAAACA AGAAGAGATT AAAGTCACAA 
GGAAAGAACT GCAAAAAGCT AGCCAAGAAT CTCCTTAAGG AGCCGGAAAA CCGCAACGAT 
TTTTGCCTCT GGAAGCAGTA TGCGCATCTG GAGTGGTTGC TTGGCAACAT GGAGGACGCC 
AGGAAGGTTT TCGATACAGC ACTCAGCATG GCAGGGAGCA GAGAACTGAA GGATCACGAA 
CTCTGTGAGC TCAGTCTGCT CTACGCTGAG CTGGAGGTGG AGCTGTTGCT AGACTCGAGA 
GGGGCCGCCA CAGCCCGAGC TGTTCACATC TTAACCAGGC TGACTGAGAA CGGGCCCTGT 
GGGCCCTACG CCGGGCAGGT CTTGGCCGTT CACATTTTGA AAGCTCGGAA GGCTTATGAA 
CACGCACTGC AGGACTGCTT GGGTGAGGGC TGTGTCTCTG GACCGGCTCC CACCAACTCC 
TTGAACTGCC TAACTAGCTT GGTCAAATGC TTTATGCTCT TTCAGTATTT GACCGTGGGG 
ATCGATGCCG CTGTGCGGAT ATACGAGCAA GTATTTGCAA AACTGAAGGG CTCTGGTTCC 
CCAGAATGCT GTGGCCAGGA TGACAGTGCC GGCACCCAGC GTCTGAGCAG TGTTCTCGAG 
GCTGTCACAC TGATGCACAC CAGCCTGCTC AGATTCCACA TGAAAGTCAG CGTTTACCCG 
CTGGCCCCTC TGCGAGAGGC ACTCTCCGAG GCTTTAAAGT TGTATCCGGG CAACCAGGTT 
CTTTGGAGGT CATATGTACA GATTCAGAAT AAGTCTCACA GTGCCAGTAA GACCAGAAGA 
TTCTTTGATG CAGTCACCAG GTCTGCTAAA CCCTTAGAGC CTTGGTTGTT TGCCATTGAA 
GCCGAGAAAA TGAGGAAGAG ACTAGTGGAA ACTGTTCAGA GGGTAGATGG TAGAGAGGTC 
CACGCCACAA TTCCTGAGAC CGGCTTGACA CATCGGATCA AAGCCCTGTT TGAAAATGCA 
ATGCGGAGTG ACAGCGGCAG CCAGTGCCCC TTACTGTGGA GGATGTATTT GAATTTTTTG 
GTTTCCTTAG GAAACAAGGA AAGAAGCAAA GGTGTGTTCT ACAAAGCACT TCAGAATTGT 
CCTTGGGCAA AGGTGTTGTA CATGGACGCT GTGGAGTACT TCCCCGATGA GATGCAGGAG 
ATCCTGGACC TGATGACTGA GAAGGAGCTC CGGGTGCGCC TGCCGGTGGA GGAGCTGGAG 
CTTCTGCTTG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( XM_005605398.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005605455.1 Equus caballus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Price & Availability↑

CloneID	OHb14026
Clone ID Related Accession (Same CDS sequence)	XM_005605398.2
Accession Version	XM_005605398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	protein NRDE2 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867395.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005605398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCCCAGCCTT TGCGGGCGTT AGTGAAGCTC CCGCTAGCGG AAGTTCTAGG 
AAAGAATTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GAAGCATACC ATCTCTGAGC 
CAACAAACTG AAGAGACCAC AGCTCTTGCT TCTGAAGGGT TGCCACTGAC AAGGAGTCCT 
CTGAAATCAG AGCCATCAGA GGAAAGTGAC ACTAACAGAA AGCTCAAATC TATAAGCAGA 
AAAAAGAAGA AAGAGAAAAA GAAGAAAAGG AAGCATCAGC ATCATAAGAA AACCAAGAGA 
AAACATGGGC AGTCGAGTAG CAGTGGATCT GAGCCAGATA CTGATTCTGA AAAGGACAGA 
TCTTACAGAA GCATCAGAGA CAGTAAAAGG GAATCAGAGA AACCAAATCA GCAAAATAAT 
GCCACTGCTG ATAGTGGACA TCGCCTTGTT TGGCTTGAGG ACGTTCAGGC TCTGACAGGA 
GAAACCTTCA GAACAGATAA GAAGCCGGAT CCTGCAAACT GGGAGTATAA GTCTCTCTAC 
CGTGGAGACA TAGCAAGGTA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCAAAG 
AAGCAGTGTA TATCTTGGGA GGGGACTTCC ACGGGAAAGA AGCATCCACA CAAGCGTGTT 
GAACGCTATT TTATGAAGAA GAATGTGGGA TTAATGAACA TTGACGGAGT TGCCGTTAGC 
AGTCAAACTG CCCCTCCCTC GTCTGAGCCC GTCTCGTTTA TCCCAGTGAA GGACTCAGAT 
GACGCGGCTC CTCCTGTTAC AGCCTGGTTG AACCCTCTGG GGATTTATGA TCAGTCCACC 
ACACAGTGGT TACAAGGAAA GGGTGCTTCA GAGCAAGAAT CAAAGCAGCC AGACTCACAG 
GTGGACAGAG AGAGCGCGCT GCTCAAGGCC AAGGTGGAGG AGTTTAACAG GAGGGTCCGG 
GAGCATCCTC GGGATGTTCA GCTGTGGATG GCGTTTGTTG CTTTTCAGGA CGAGGTCATG 
AAAAGTCCGG GCCTGTATGC CATCGAGGAA GGAGAGCAGG AAAAGCGAAA GAGGTCCCTG 
AAGCTGCTTC TGGAGAAGAA GCTGGCCATC CTGGAGCGGG CCATTGAAAG CAACCCGAGC 
TGCGTGGACC TGAAGCTCGC CAAGCTGCAG CTCTGCACGG AGCTCTGGGA GCCCGCCACC 
CTGCTCAGGG AGTGGCAGAA ACTCATATTC TTACATCCCA ACAATACAGC CCTTTGGCAT 
AAATATCTTT TATTTTGCCA GAGCCAGTTT AGCACCTTTT CAATATCAAA AATTCACAGT 
CTTTATGGAA AGTGCTTGAG TACCCTGTCT GCTGTTAAGG ATGGCAGCAT CTTGTCTCAC 
CCTGAGTTGC CCGGCACCGA GGAGGCCATG TTTGCCCTCT TTCTTCAGCA GTGCCACTTT 
CTGCGGCAGG CCGGTCACTC TGAGAAGGCC GTCTCTCTGT TCCAGGCCAT GGTCGACTTC 
ACCTTCTTCA AACCGGACAG TGTGAAAGGG CTGCCTACCA AAGGCCAGGT GGAGTTCTTT 
GAGCCCTTTT GGGACAGTGG AGAGCCCCGG GCTGGGGAGA AGGGCGCCCG GGGCTGGAGA 
GCGTGGATGC ACCAGCAGGA GCGGGGTGGC TGGGTGGTCA TCAGTCCAGA TGATGAGGAG 
GAAGAACCAG AAGATGATGA TCAGGAAATA AGAGATAAGA CTCTGCCCAG GTGGCAGATC 
TGGCTTGCTG CTGAGCGGTC CCGAGACCAG AGGCACTGGC GGCCGTGGCG CCCAGATAAG 
ACCAAGAAGC AGACTGAGGA AGACTGTGAG GATCCAGAGA GACAGGTGTT GTTTGATGAT 
ATTGGACAAT CTCTGATCCG ACTTTCCAGC CAGGATCTTC AGTTTCGGCT GATTGCGGCC 
TTCCTGCAGT TCTTGGGCGT GCCTTGTGGC GTCAGCCCTC CCGCCTCCTG CCTTTATCTG 
GCCATGGACG AGAACAGCAT CTTTGATAAC GGACTTTGTG ATGAAAAGCC GTTGACTTTC 
CTCAACCTGT CATTTTCTGG CGTCAGCTGT GTTGGCCGCA TGGACCAGTT GGGCTGCCGG 
CGCTGGACCA GAGCTCACGG TCGAGAGGGC GAGGACTTCA TCCGCAACGT CTTCCACCTG 
GTGCTGCCTT TGTTTTCCGG CAAGGAGAGA TCTCAGCTCT GCCTCTCCTG GTTACAGTAC 
GAGATTGCAA AGGTCATCTG GTGTCTGCAC ACTAAAAACA AGAAGAGATT AAAGTCACAA 
GGAAAGAACT GCAAAAAGCT AGCCAAGAAT CTCCTTAAGG AGCCGGAAAA CCGCAACGAT 
TTTTGCCTCT GGAAGCAGTA TGCGCATCTG GAGTGGTTGC TTGGCAACAT GGAGGACGCC 
AGGAAGGTTT TCGATACAGC ACTCAGCATG GCAGGGAGCA GAGAACTGAA GGATCACGAA 
CTCTGTGAGC TCAGTCTGCT CTACGCTGAG CTGGAGGTGG AGCTGTTGCT AGACTCGAGA 
GGGGCCGCCA CAGCCCGAGC TGTTCACATC TTAACCAGGC TGACTGAGAA CGGGCCCTGT 
GGGCCCTACG CCGGGCAGGT CTTGGCCGTT CACATTTTGA AAGCTCGGAA GGCTTATGAA 
CACGCACTGC AGGACTGCTT GGGTGAGGGC TGTGTCTCTG GACCGGCTCC CACCAACTCC 
TTGAACTGCC TAACTAGCTT GGTCAAATGC TTTATGCTCT TTCAGTATTT GACCGTGGGG 
ATCGATGCCG CTGTGCGGAT ATACGAGCAA GTATTTGCAA AACTGAAGGG CTCTGGTTCC 
CCAGAATGCT GTGGCCAGGA TGACAGTGCC GGCACCCAGC GTCTGAGCAG TGTTCTCGAG 
GCTGTCACAC TGATGCACAC CAGCCTGCTC AGATTCCACA TGAAAGTCAG CGTTTACCCG 
CTGGCCCCTC TGCGAGAGGC ACTCTCCGAG GCTTTAAAGT TGTATCCGGG CAACCAGGTT 
CTTTGGAGGT CATATGTACA GATTCAGAAT AAGTCTCACA GTGCCAGTAA GACCAGAAGA 
TTCTTTGATG CAGTCACCAG GTCTGCTAAA CCCTTAGAGC CTTGGTTGTT TGCCATTGAA 
GCCGAGAAAA TGAGGAAGAG ACTAGTGGAA ACTGTTCAGA GGGTAGATGG TAGAGAGGTC 
CACGCCACAA TTCCTGAGAC CGGCTTGACA CATCGGATCA AAGCCCTGTT TGAAAATGCA 
ATGCGGAGTG ACAGCGGCAG CCAGTGCCCC TTACTGTGGA GGATGTATTT GAATTTTTTG 
GTTTCCTTAG GAAACAAGGA AAGAAGCAAA GGTGTGTTCT ACAAAGCACT TCAGAATTGT 
CCTTGGGCAA AGGTGTTGTA CATGGACGCT GTGGAGTACT TCCCCGATGA GATGCAGGAG 
ATCCTGGACC TGATGACTGA GAAGGAGCTC CGGGTGCGCC TGCCGGTGGA GGAGCTGGAG 
CTTCTGCTTG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005605455.1
CDS	76..3573
Translation	MALFPAFAGVSEAPASGSSRKELDWLSNPSFCVGSIPSLSQQTEETTALASEGLPLTRSPLKSEPSEESDTNRKLKSISRKKKKEKKKKRKHQHHKKTKRKHGQSSSSGSEPDTDSEKDRSYRSIRDSKRESEKPNQQNNATADSGHRLVWLEDVQALTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTGKKHPHKRVERYFMKKNVGLMNIDGVAVSSQTAPPSSEPVSFIPVKDSDDAAPPVTAWLNPLGIYDQSTTQWLQGKGASEQESKQPDSQVDRESALLKAKVEEFNRRVREHPRDVQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLLLEKKLAILERAIESNPSCVDLKLAKLQLCTELWEPATLLREWQKLIFLHPNNTALWHKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPELPGTEEAMFALFLQQCHFLRQAGHSEKAVSLFQAMVDFTFFKPDSVKGLPTKGQVEFFEPFWDSGEPRAGEKGARGWRAWMHQQERGGWVVISPDDEEEEPEDDDQEIRDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSQDLQFRLIAAFLQFLGVPCGVSPPASCLYLAMDENSIFDNGLCDEKPLTFLNLSFSGVSCVGRMDQLGCRRWTRAHGREGEDFIRNVFHLVLPLFSGKERSQLCLSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENRNDFCLWKQYAHLEWLLGNMEDARKVFDTALSMAGSRELKDHELCELSLLYAELEVELLLDSRGAATARAVHILTRLTENGPCGPYAGQVLAVHILKARKAYEHALQDCLGEGCVSGPAPTNSLNCLTSLVKCFMLFQYLTVGIDAAVRIYEQVFAKLKGSGSPECCGQDDSAGTQRLSSVLEAVTLMHTSLLRFHMKVSVYPLAPLREALSEALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDAVTRSAKPLEPWLFAIEAEKMRKRLVETVQRVDGREVHATIPETGLTHRIKALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYMDAVEYFPDEMQEILDLMTEKELRVRLPVEELELLLED

Target ORF information:

RefSeq Version	XM_005605398.2
Organism	Equus caballus(horse)
Definition	Equus caballus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005605398.2

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGCGTT AGTGAAGCTC CCGCTAGCGG AAGTTCTAGG 
AAAGAATTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GAAGCATACC ATCTCTGAGC 
CAACAAACTG AAGAGACCAC AGCTCTTGCT TCTGAAGGGT TGCCACTGAC AAGGAGTCCT 
CTGAAATCAG AGCCATCAGA GGAAAGTGAC ACTAACAGAA AGCTCAAATC TATAAGCAGA 
AAAAAGAAGA AAGAGAAAAA GAAGAAAAGG AAGCATCAGC ATCATAAGAA AACCAAGAGA 
AAACATGGGC AGTCGAGTAG CAGTGGATCT GAGCCAGATA CTGATTCTGA AAAGGACAGA 
TCTTACAGAA GCATCAGAGA CAGTAAAAGG GAATCAGAGA AACCAAATCA GCAAAATAAT 
GCCACTGCTG ATAGTGGACA TCGCCTTGTT TGGCTTGAGG ACGTTCAGGC TCTGACAGGA 
GAAACCTTCA GAACAGATAA GAAGCCGGAT CCTGCAAACT GGGAGTATAA GTCTCTCTAC 
CGTGGAGACA TAGCAAGGTA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCAAAG 
AAGCAGTGTA TATCTTGGGA GGGGACTTCC ACGGGAAAGA AGCATCCACA CAAGCGTGTT 
GAACGCTATT TTATGAAGAA GAATGTGGGA TTAATGAACA TTGACGGAGT TGCCGTTAGC 
AGTCAAACTG CCCCTCCCTC GTCTGAGCCC GTCTCGTTTA TCCCAGTGAA GGACTCAGAT 
GACGCGGCTC CTCCTGTTAC AGCCTGGTTG AACCCTCTGG GGATTTATGA TCAGTCCACC 
ACACAGTGGT TACAAGGAAA GGGTGCTTCA GAGCAAGAAT CAAAGCAGCC AGACTCACAG 
GTGGACAGAG AGAGCGCGCT GCTCAAGGCC AAGGTGGAGG AGTTTAACAG GAGGGTCCGG 
GAGCATCCTC GGGATGTTCA GCTGTGGATG GCGTTTGTTG CTTTTCAGGA CGAGGTCATG 
AAAAGTCCGG GCCTGTATGC CATCGAGGAA GGAGAGCAGG AAAAGCGAAA GAGGTCCCTG 
AAGCTGCTTC TGGAGAAGAA GCTGGCCATC CTGGAGCGGG CCATTGAAAG CAACCCGAGC 
TGCGTGGACC TGAAGCTCGC CAAGCTGCAG CTCTGCACGG AGCTCTGGGA GCCCGCCACC 
CTGCTCAGGG AGTGGCAGAA ACTCATATTC TTACATCCCA ACAATACAGC CCTTTGGCAT 
AAATATCTTT TATTTTGCCA GAGCCAGTTT AGCACCTTTT CAATATCAAA AATTCACAGT 
CTTTATGGAA AGTGCTTGAG TACCCTGTCT GCTGTTAAGG ATGGCAGCAT CTTGTCTCAC 
CCTGAGTTGC CCGGCACCGA GGAGGCCATG TTTGCCCTCT TTCTTCAGCA GTGCCACTTT 
CTGCGGCAGG CCGGTCACTC TGAGAAGGCC GTCTCTCTGT TCCAGGCCAT GGTCGACTTC 
ACCTTCTTCA AACCGGACAG TGTGAAAGGG CTGCCTACCA AAGGCCAGGT GGAGTTCTTT 
GAGCCCTTTT GGGACAGTGG AGAGCCCCGG GCTGGGGAGA AGGGCGCCCG GGGCTGGAGA 
GCGTGGATGC ACCAGCAGGA GCGGGGTGGC TGGGTGGTCA TCAGTCCAGA TGATGAGGAG 
GAAGAACCAG AAGATGATGA TCAGGAAATA AGAGATAAGA CTCTGCCCAG GTGGCAGATC 
TGGCTTGCTG CTGAGCGGTC CCGAGACCAG AGGCACTGGC GGCCGTGGCG CCCAGATAAG 
ACCAAGAAGC AGACTGAGGA AGACTGTGAG GATCCAGAGA GACAGGTGTT GTTTGATGAT 
ATTGGACAAT CTCTGATCCG ACTTTCCAGC CAGGATCTTC AGTTTCGGCT GATTGCGGCC 
TTCCTGCAGT TCTTGGGCGT GCCTTGTGGC GTCAGCCCTC CCGCCTCCTG CCTTTATCTG 
GCCATGGACG AGAACAGCAT CTTTGATAAC GGACTTTGTG ATGAAAAGCC GTTGACTTTC 
CTCAACCTGT CATTTTCTGG CGTCAGCTGT GTTGGCCGCA TGGACCAGTT GGGCTGCCGG 
CGCTGGACCA GAGCTCACGG TCGAGAGGGC GAGGACTTCA TCCGCAACGT CTTCCACCTG 
GTGCTGCCTT TGTTTTCCGG CAAGGAGAGA TCTCAGCTCT GCCTCTCCTG GTTACAGTAC 
GAGATTGCAA AGGTCATCTG GTGTCTGCAC ACTAAAAACA AGAAGAGATT AAAGTCACAA 
GGAAAGAACT GCAAAAAGCT AGCCAAGAAT CTCCTTAAGG AGCCGGAAAA CCGCAACGAT 
TTTTGCCTCT GGAAGCAGTA TGCGCATCTG GAGTGGTTGC TTGGCAACAT GGAGGACGCC 
AGGAAGGTTT TCGATACAGC ACTCAGCATG GCAGGGAGCA GAGAACTGAA GGATCACGAA 
CTCTGTGAGC TCAGTCTGCT CTACGCTGAG CTGGAGGTGG AGCTGTTGCT AGACTCGAGA 
GGGGCCGCCA CAGCCCGAGC TGTTCACATC TTAACCAGGC TGACTGAGAA CGGGCCCTGT 
GGGCCCTACG CCGGGCAGGT CTTGGCCGTT CACATTTTGA AAGCTCGGAA GGCTTATGAA 
CACGCACTGC AGGACTGCTT GGGTGAGGGC TGTGTCTCTG GACCGGCTCC CACCAACTCC 
TTGAACTGCC TAACTAGCTT GGTCAAATGC TTTATGCTCT TTCAGTATTT GACCGTGGGG 
ATCGATGCCG CTGTGCGGAT ATACGAGCAA GTATTTGCAA AACTGAAGGG CTCTGGTTCC 
CCAGAATGCT GTGGCCAGGA TGACAGTGCC GGCACCCAGC GTCTGAGCAG TGTTCTCGAG 
GCTGTCACAC TGATGCACAC CAGCCTGCTC AGATTCCACA TGAAAGTCAG CGTTTACCCG 
CTGGCCCCTC TGCGAGAGGC ACTCTCCGAG GCTTTAAAGT TGTATCCGGG CAACCAGGTT 
CTTTGGAGGT CATATGTACA GATTCAGAAT AAGTCTCACA GTGCCAGTAA GACCAGAAGA 
TTCTTTGATG CAGTCACCAG GTCTGCTAAA CCCTTAGAGC CTTGGTTGTT TGCCATTGAA 
GCCGAGAAAA TGAGGAAGAG ACTAGTGGAA ACTGTTCAGA GGGTAGATGG TAGAGAGGTC 
CACGCCACAA TTCCTGAGAC CGGCTTGACA CATCGGATCA AAGCCCTGTT TGAAAATGCA 
ATGCGGAGTG ACAGCGGCAG CCAGTGCCCC TTACTGTGGA GGATGTATTT GAATTTTTTG 
GTTTCCTTAG GAAACAAGGA AAGAAGCAAA GGTGTGTTCT ACAAAGCACT TCAGAATTGT 
CCTTGGGCAA AGGTGTTGTA CATGGACGCT GTGGAGTACT TCCCCGATGA GATGCAGGAG 
ATCCTGGACC TGATGACTGA GAAGGAGCTC CGGGTGCGCC TGCCGGTGGA GGAGCTGGAG 
CTTCTGCTTG AGGACTAG