| Gene Symbol | LOC100034242 |
| Entrez Gene ID | 100034242 |
| Full Name | alcohol dehydrogenase-E-isoenzyme |
| Gene Type | protein-coding |
| Organism | Equus caballus(horse) |
ORF » Species Summary » Equus caballus » LOC100034242 cDNA ORF clone
| Gene Symbol | LOC100034242 |
| Entrez Gene ID | 100034242 |
| Full Name | alcohol dehydrogenase-E-isoenzyme |
| Gene Type | protein-coding |
| Organism | Equus caballus(horse) |
| mRNA | Protein | Name |
|---|---|---|
| NM_001082528.1 | NP_001075997.1 | alcohol dehydrogenase E chain |
Inversion of substrate stereoselectivity of horse liver alcohol dehydrogenase by substitutions of Ser-48 and Phe-93.
Kim K, Plapp BV
Chemico-biological interactions27677-87(2017 Oct)
Horse Liver Alcohol Dehydrogenase: Zinc Coordination and Catalysis.
Plapp BV, Savarimuthu BR, Ferraro DJ, Rubach JK, Brown EN, Ramaswamy S
Biochemistry56(28)3632-3646(2017 07)
Annotation of the Protein Coding Regions of the Equine Genome.
Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando L, MacLeod JN
PloS one10(6)e0124375(2015)
Atomic-resolution structures of horse liver alcohol dehydrogenase with NAD(+) and fluoroalcohols define strained Michaelis complexes.
Plapp BV, Ramaswamy S
Biochemistry51(19)4035-48(2012 May)
Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 A resolution.
Al-Karadaghi S, Cedergren-Zeppezauer ES, H?vmoller S
Acta crystallographica. Section D, Biological crystallography50(Pt 6)793-807(1994 Nov)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The enzyme-NADH complex has a closed conformation similar to that of ternary complexes with coenzyme and substrate analogues; the coordination of the catalytic zinc is similar to that found in the apoenzyme, except that a minor, alternative position for the catalytic zinc is approximately 1.3 A from the major position and closer to Glu-68, which could form the alternative coordination to the catalytic zinc.
Title: Horse Liver Alcohol Dehydrogenase: Zinc Coordination and Catalysis.
The following LOC100034242 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100034242 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHb28074 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001082528.1 | |
| Accession Version | NM_001082528.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Equus caballus(horse) | |
| Product | alcohol dehydrogenase E chain | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M64864.1. ##Evidence-Data-START## Transcript exon combination :: M64864.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2452530, SAMEA2452531 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGCACAG CAGGAAAAGT AATAAAATGC AAAGCGGCTG TGCTGTGGGA GGAAAAGAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001075997.1 |
| CDS | 13..1140 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001082528.1 |
1 | ATGAGCACAG CAGGAAAAGT AATAAAATGC AAAGCGGCTG TGCTGTGGGA GGAAAAGAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Inversion of substrate stereoselectivity of horse liver alcohol dehydrogenase by substitutions of Ser-48 and Phe-93. |
Horse Liver Alcohol Dehydrogenase: Zinc Coordination and Catalysis. |
Annotation of the Protein Coding Regions of the Equine Genome. |
Atomic-resolution structures of horse liver alcohol dehydrogenase with NAD(+) and fluoroalcohols define strained Michaelis complexes. |
Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 A resolution. |