Pde6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde6
Entrez Gene ID	41760
Full Name	Phosphodiesterase 6
Synonyms	CG8279,DmPDE5/6,DmPDE6,Dmel\CG8279,PDE6
General protein information	Preferred Names Phosphodiesterase 6 Names CG8279 gene product from transcript CG8279-RB CG8279-PB CG8279-PC CG8279-PD Pde6-PB Pde6-PC Pde6-PD phosphodiesterase 6
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88C3-88C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_142112.5	NP_650369.3	phosphodiesterase 6, isoform B
NM_001275637.2	NP_001262566.1	phosphodiesterase 6, isoform C
NM_001300398.1	NP_001287327.1	phosphodiesterase 6, isoform D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDE11A	XP_005640467.1
	PDE11A	XP_001097592.1
Danio rerio (zebrafish)	pde11a	XP_689838.4
Drosophila melanogaster (fruit fly)	Pde6	NP_650369.3
Homo sapiens (human)	PDE11A	NP_058649.3
Gallus gallus (chicken)	PDE11A	XP_421985.4
Bos taurus (cattle)	PDE11A	NP_001192351.1
Mus musculus (house mouse)	Pde11a	NP_001074502.1
Rattus norvegicus (Norway rat)	Pde11a	NP_543169.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004119	XP_312997.5
Xenopus tropicalis (tropical clawed frog)	pde11a	XP_002934646.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase-5/6 (DmPDE5/6) by prenylation and interaction with a prenyl-binding protein.
Title: Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase by prenylation and interaction with a prenyl-binding protein.

The first demonstration of a novel role for a cG-PDE in modulating cGMP transport and efflux in D. melanogaster Malphigian tubules is presented.
Title: A novel role for a Drosophila homologue of cGMP-specific phosphodiesterase in the active transport of cGMP.

The Drosophila genome encodes five novel PDE genes in addition to dunce. Predicted PDE sequences of Drosophila show highly conserved critical domains when compared with human PDEs.
Title: Cyclic nucleotide phosphodiesterases in Drosophila melanogaster.

The following Pde6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04872	NM_001300398.1 Latest version!	Drosophila melanogaster phosphodiesterase 6, transcript variant D (Pde6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04872	NM_142112.5 Latest version!	Drosophila melanogaster phosphodiesterase 6, transcript variant B (Pde6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07987	NM_001275637.2 Latest version!	Drosophila melanogaster phosphodiesterase 6, transcript variant C (Pde6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde6 ( NM_142112.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650369.3 Drosophila melanogaster phosphodiesterase 6, transcript variant B (Pde6), mRNA.

Price & Availability↑

CloneID	OFa04872
Clone ID Related Accession (Same CDS sequence)	NM_142112.5 , NM_001300398.1
Accession Version	NM_142112.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 6, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142112.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATG TGTCATCGCC AGCTGGTGGC GCCGCATCTC CGGTTGAAAT GTCCACCACC 
AGCAGCTCTT CAGCCGCCAC TACGTCGGCG TCCTCGTCCA AACCGCTGAC CAATGGCGCC 
AACAAGACGG CTATATCAAC GGCAGCTGGA GGAGTGACAC CGGGTGCTGT TCCTGGACCC 
GGGTCCGGAG CAATCCCCGC CAGCAGTTCG AGTGGCAATC AGGTCAAACT GGAGCATCAT 
CACCGCCAGA GCAATAACAA TCGACCTGCG GTTACCAATC GCAGTTCGGA AACCAAGCTG 
ATGACACCTA CCGGCAGTTC CTCATCCCCA TCGCAATCTC CATCCCAAAC CCAAGCCAGT 
ATACAAACCC AAACGAGTCA GCAGGACAGG CTGGCCAAGG CGAGCACCAC GGCATCCCAG 
CAGGATGTCG ATGAGGTGGC CCGCCTTTTC GAGGAGAAGC CGGAAGCATT CGAGAAGTGG 
CTGACGGAAA GGGCTCCTCC AGAGGCACTC AGCCGACTGC AGGAGTTCAT TGAGAATCGC 
AAGCCGCACA AGCGACCCTC GGTCACCTCC GATCTCTTCC AGCAGTGGAT GGCCGCATCG 
CCCACCGTGC AGCAGAAATC GCCGAGGAGT CTCTCGAACT CTTCAGCATC GTCACTTCCG 
GAGTGCCGGA GGCATCTGAT GGACCTGGAC GAGGGCGAGC TGTTCATGGA ACTGATTCGC 
GACGTGGCCA ACGAGCTGGA CATCGATGTC CTCTGCCACA AGATTCTGGT CAATGTCGGA 
CTGCTCACCC ACGCGGATCG TGGCTCCCTG TTTCTGGCCA AGGGGACGCC CACCAACAAA 
TATCTGGTGG CCAAGCTATT CGATGTCACT CAAAAGACAG CACTCAAGGA CGCGGTGACT 
CGGGCCAGTG CCGAGGAGAT AATCATTCCC TTTGGCATTG GAATCGCGGG AATGGTGGCG 
CAGACAAAGC AGATGATTAA CATCAAGGAG GCCTACAAGG ATGCTCGCTT CAATTGCGAA 
ATCGATCTGA AGACCGGCTA CAAAACGAAC GCCATCCTCT GCATGCCCAT TTGCAATTAC 
GAGGGCGACA TCATAGGCGT GGCGCAAATT ATTAACAAAA CGAATGGCTG CATGGAGTTT 
GACGAGCACG ATGTGGAAAT TTTTCGCCGC TATTTGACCT TTTGTGGAAT TGGCATACAG 
AATGCCCAGC TCTTTGAGAT GTCCGTCCAG GAATACCGGC GTAATCAAAT TCTCCTCAAT 
TTGGCGCGCA GTATCTTCGA GGAGCAGAAT AATCTTGAGT GCTTAGTTAC CAAAATTATG 
ACAGAGGCCC GGGAACTACT CAAATGCGAA AGGTGCTCTG TGTTTCTAGT GGATCTCGAC 
TGTTGCGAGG CGAGCCATTT GGAGAAGATA ATTGAGAAGC CCAATCAGCC AGCCACCCGC 
GCCATCAAGT CCGCCGATAG CTTCGAGGAG AAGAAAATGC GCAATCGTTT CACGGTGCTC 
TTCGAACTCG GTGGAGAATA TCAGGCTGCC AATGTTTCGA GGCCATCTGT CAGCGAGTTG 
AGCTCCTCGA CGCTGGCCCA GATTGCTCAG TTTGTGGCCA CCACCGGGCA AACGGTGAAC 
ATCTGCGATG TCATCGAATG GGTCAGGGAT CACAATCAAA TTCGAGCGGA GGACGAAATA 
GATAGCACCC AGGCCATCCT CTGCATGCCC ATTATGAATG CACAGAAGAA GGTCATTGGC 
GTTGCCCAGC TTATCAACAA GGCCAACGGA GTTCCCTTCA CCGATTCGGA TGCCTCTATT 
TTCGAGGCAT TTGCCATTTT CTGTGGCTTG GGCATTCACA ACACCCAGAT GTACGAGAAT 
GCCTGCAAGT TGATGGCCAA GCAGAAGGTG GCCCTCGAGT GCCTCAGTTA CCATGCCACC 
GCTAGTCAGG ATCAGACGGA GAAGCTCACC CAGGACGTGA TTGCCGAGGC GGAGTCCTAC 
AACCTGTACA GCTTCACCTT TACGGACTTT GAGCTGGTCG ACGATGACAC CTGTCGCGCA 
GTGCTTCGCA TGTTTATGCA GTGCAATTTG GTCTCGCAGT TTCAGATACC ATATGATGTA 
CTTTGCCGCT GGGTTTTAAG TGTTCGCAAG AATTATCGTC CTGTTAAATA TCACAATTGG 
AGGCATGCTC TAAACGTGGC CCAAACGATG TTCGCCATGC TTAAAACTGG AAAGATGGAG 
CGCTTTATGA CAGATCTGGA GATTCTGGGT CTGTTGGTGG CCTGTTTGTG CCATGACTTG 
GATCATCGTG GCACCAACAA TGCATTCCAG ACCAAAACGG AGTCCCCGCT GGCCATTTTG 
TACACCACTA GCACGATGGA GCACCATCAC TTCGATCAGT GCGTAATGAT TCTCAATTCG 
GAGGGAAATA ATATATTTCA GGCCTTGTCA CCTGAGGACT ATCGTTCAGT CATGAAGACC 
GTGGAAAGCG CCATTCTGTC CACCGATCTG GCCATGTATT TCAAGAAAAG GAACGCTTTC 
CTAGAGTTGG TGGAAAATGG GGAATTCGAT TGGCAGGGCG AGGAGAAGAA AGATTTGCTT 
TGTGGCATGA TGATGACTGC TTGCGATGTG AGTGCCATTG CCAAGCCCTG GGAGGTTCAG 
CACAAGGTTG CCAAGTTGGT GGCCGATGAG TTCTTTGACC AAGGAGATCT CGAAAAGCTG 
CAGCTTAACA CCCAACCAGT CGCCATGATG GACAGGGAGC GCAAGGACGA GCTACCCAAG 
ATGCAGGTGG GCTTTATCGA CGTGATCTGT CTACCACTCT ACAGAGTGCT TTGCGACACC 
TTTCCCTGGA TCACTCCGCT CTATGAAGGA ACCTTGGAGA ACCGACGCAA CTGGCAGGAT 
CTGGCCGAGA AAGTGGAAAT GGGCCTCACA TGGATCGATC ACGATACGAT TGACAAACCC 
GTGGAGGAGT TTGCCGCGTG TGCGGATGAA GAAATCAAGG ATATCGAGTT CACTGTGACC 
ACGCTAAATT GCAATCAGCA ATCTCAGCAT GGCAGCGAGG ATAGCCACAC GCCGGAGCAC 
CAGCGAAGTG GCTCCAGATT GAGCATGAAG AAGACCGGAG CCCTGGGCAA GGCAGTTCGA 
TCGAAGCTAT CAAAGACTCT ATACAACAGC ATGGATGGCT CAAAGCCGAA GACCTCCTTG 
AAGCTCCTTG AGTCCCATGT CAGCGAGGAT ATGGATGACA AAAGTCCTAC GAGTCCTTCG 
CAGCCGCAGG CCTCCGGGAG CATGGGCCGC ATGTCCGCCT CGTCCTCCAC CTCATCTGCC 
GGCGGCCAGA TGGTGGACAA GTCCAAAAAG CGTTCCAAAC TGTGCGCGTT GTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650369.3
CDS	1269..4625
Translation	MTDVSSPAGGAASPVEMSTTSSSSAATTSASSSKPLTNGANKTAISTAAGGVTPGAVPGPGSGAIPASSSSGNQVKLEHHHRQSNNNRPAVTNRSSETKLMTPTGSSSSPSQSPSQTQASIQTQTSQQDRLAKASTTASQQDVDEVARLFEEKPEAFEKWLTERAPPEALSRLQEFIENRKPHKRPSVTSDLFQQWMAASPTVQQKSPRSLSNSSASSLPECRRHLMDLDEGELFMELIRDVANELDIDVLCHKILVNVGLLTHADRGSLFLAKGTPTNKYLVAKLFDVTQKTALKDAVTRASAEEIIIPFGIGIAGMVAQTKQMINIKEAYKDARFNCEIDLKTGYKTNAILCMPICNYEGDIIGVAQIINKTNGCMEFDEHDVEIFRRYLTFCGIGIQNAQLFEMSVQEYRRNQILLNLARSIFEEQNNLECLVTKIMTEARELLKCERCSVFLVDLDCCEASHLEKIIEKPNQPATRAIKSADSFEEKKMRNRFTVLFELGGEYQAANVSRPSVSELSSSTLAQIAQFVATTGQTVNICDVIEWVRDHNQIRAEDEIDSTQAILCMPIMNAQKKVIGVAQLINKANGVPFTDSDASIFEAFAIFCGLGIHNTQMYENACKLMAKQKVALECLSYHATASQDQTEKLTQDVIAEAESYNLYSFTFTDFELVDDDTCRAVLRMFMQCNLVSQFQIPYDVLCRWVLSVRKNYRPVKYHNWRHALNVAQTMFAMLKTGKMERFMTDLEILGLLVACLCHDLDHRGTNNAFQTKTESPLAILYTTSTMEHHHFDQCVMILNSEGNNIFQALSPEDYRSVMKTVESAILSTDLAMYFKKRNAFLELVENGEFDWQGEEKKDLLCGMMMTACDVSAIAKPWEVQHKVAKLVADEFFDQGDLEKLQLNTQPVAMMDRERKDELPKMQVGFIDVICLPLYRVLCDTFPWITPLYEGTLENRRNWQDLAEKVEMGLTWIDHDTIDKPVEEFAACADEEIKDIEFTVTTLNCNQQSQHGSEDSHTPEHQRSGSRLSMKKTGALGKAVRSKLSKTLYNSMDGSKPKTSLKLLESHVSEDMDDKSPTSPSQPQASGSMGRMSASSSTSSAGGQMVDKSKKRSKLCALL

Target ORF information:

RefSeq Version	NM_142112.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 6, transcript variant B (Pde6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142112.5

ORF Insert Sequence:

ATGACAGATG TGTCATCGCC AGCTGGTGGC GCCGCATCTC CGGTTGAAAT GTCCACCACC 
AGCAGCTCTT CAGCCGCCAC TACGTCGGCG TCCTCGTCCA AACCGCTGAC CAATGGCGCC 
AACAAGACGG CTATATCAAC GGCAGCTGGA GGAGTGACAC CGGGTGCTGT TCCTGGACCC 
GGGTCCGGAG CAATCCCCGC CAGCAGTTCG AGTGGCAATC AGGTCAAACT GGAGCATCAT 
CACCGCCAGA GCAATAACAA TCGACCTGCG GTTACCAATC GCAGTTCGGA AACCAAGCTG 
ATGACACCTA CCGGCAGTTC CTCATCCCCA TCGCAATCTC CATCCCAAAC CCAAGCCAGT 
ATACAAACCC AAACGAGTCA GCAGGACAGG CTGGCCAAGG CGAGCACCAC GGCATCCCAG 
CAGGATGTCG ATGAGGTGGC CCGCCTTTTC GAGGAGAAGC CGGAAGCATT CGAGAAGTGG 
CTGACGGAAA GGGCTCCTCC AGAGGCACTC AGCCGACTGC AGGAGTTCAT TGAGAATCGC 
AAGCCGCACA AGCGACCCTC GGTCACCTCC GATCTCTTCC AGCAGTGGAT GGCCGCATCG 
CCCACCGTGC AGCAGAAATC GCCGAGGAGT CTCTCGAACT CTTCAGCATC GTCACTTCCG 
GAGTGCCGGA GGCATCTGAT GGACCTGGAC GAGGGCGAGC TGTTCATGGA ACTGATTCGC 
GACGTGGCCA ACGAGCTGGA CATCGATGTC CTCTGCCACA AGATTCTGGT CAATGTCGGA 
CTGCTCACCC ACGCGGATCG TGGCTCCCTG TTTCTGGCCA AGGGGACGCC CACCAACAAA 
TATCTGGTGG CCAAGCTATT CGATGTCACT CAAAAGACAG CACTCAAGGA CGCGGTGACT 
CGGGCCAGTG CCGAGGAGAT AATCATTCCC TTTGGCATTG GAATCGCGGG AATGGTGGCG 
CAGACAAAGC AGATGATTAA CATCAAGGAG GCCTACAAGG ATGCTCGCTT CAATTGCGAA 
ATCGATCTGA AGACCGGCTA CAAAACGAAC GCCATCCTCT GCATGCCCAT TTGCAATTAC 
GAGGGCGACA TCATAGGCGT GGCGCAAATT ATTAACAAAA CGAATGGCTG CATGGAGTTT 
GACGAGCACG ATGTGGAAAT TTTTCGCCGC TATTTGACCT TTTGTGGAAT TGGCATACAG 
AATGCCCAGC TCTTTGAGAT GTCCGTCCAG GAATACCGGC GTAATCAAAT TCTCCTCAAT 
TTGGCGCGCA GTATCTTCGA GGAGCAGAAT AATCTTGAGT GCTTAGTTAC CAAAATTATG 
ACAGAGGCCC GGGAACTACT CAAATGCGAA AGGTGCTCTG TGTTTCTAGT GGATCTCGAC 
TGTTGCGAGG CGAGCCATTT GGAGAAGATA ATTGAGAAGC CCAATCAGCC AGCCACCCGC 
GCCATCAAGT CCGCCGATAG CTTCGAGGAG AAGAAAATGC GCAATCGTTT CACGGTGCTC 
TTCGAACTCG GTGGAGAATA TCAGGCTGCC AATGTTTCGA GGCCATCTGT CAGCGAGTTG 
AGCTCCTCGA CGCTGGCCCA GATTGCTCAG TTTGTGGCCA CCACCGGGCA AACGGTGAAC 
ATCTGCGATG TCATCGAATG GGTCAGGGAT CACAATCAAA TTCGAGCGGA GGACGAAATA 
GATAGCACCC AGGCCATCCT CTGCATGCCC ATTATGAATG CACAGAAGAA GGTCATTGGC 
GTTGCCCAGC TTATCAACAA GGCCAACGGA GTTCCCTTCA CCGATTCGGA TGCCTCTATT 
TTCGAGGCAT TTGCCATTTT CTGTGGCTTG GGCATTCACA ACACCCAGAT GTACGAGAAT 
GCCTGCAAGT TGATGGCCAA GCAGAAGGTG GCCCTCGAGT GCCTCAGTTA CCATGCCACC 
GCTAGTCAGG ATCAGACGGA GAAGCTCACC CAGGACGTGA TTGCCGAGGC GGAGTCCTAC 
AACCTGTACA GCTTCACCTT TACGGACTTT GAGCTGGTCG ACGATGACAC CTGTCGCGCA 
GTGCTTCGCA TGTTTATGCA GTGCAATTTG GTCTCGCAGT TTCAGATACC ATATGATGTA 
CTTTGCCGCT GGGTTTTAAG TGTTCGCAAG AATTATCGTC CTGTTAAATA TCACAATTGG 
AGGCATGCTC TAAACGTGGC CCAAACGATG TTCGCCATGC TTAAAACTGG AAAGATGGAG 
CGCTTTATGA CAGATCTGGA GATTCTGGGT CTGTTGGTGG CCTGTTTGTG CCATGACTTG 
GATCATCGTG GCACCAACAA TGCATTCCAG ACCAAAACGG AGTCCCCGCT GGCCATTTTG 
TACACCACTA GCACGATGGA GCACCATCAC TTCGATCAGT GCGTAATGAT TCTCAATTCG 
GAGGGAAATA ATATATTTCA GGCCTTGTCA CCTGAGGACT ATCGTTCAGT CATGAAGACC 
GTGGAAAGCG CCATTCTGTC CACCGATCTG GCCATGTATT TCAAGAAAAG GAACGCTTTC 
CTAGAGTTGG TGGAAAATGG GGAATTCGAT TGGCAGGGCG AGGAGAAGAA AGATTTGCTT 
TGTGGCATGA TGATGACTGC TTGCGATGTG AGTGCCATTG CCAAGCCCTG GGAGGTTCAG 
CACAAGGTTG CCAAGTTGGT GGCCGATGAG TTCTTTGACC AAGGAGATCT CGAAAAGCTG 
CAGCTTAACA CCCAACCAGT CGCCATGATG GACAGGGAGC GCAAGGACGA GCTACCCAAG 
ATGCAGGTGG GCTTTATCGA CGTGATCTGT CTACCACTCT ACAGAGTGCT TTGCGACACC 
TTTCCCTGGA TCACTCCGCT CTATGAAGGA ACCTTGGAGA ACCGACGCAA CTGGCAGGAT 
CTGGCCGAGA AAGTGGAAAT GGGCCTCACA TGGATCGATC ACGATACGAT TGACAAACCC 
GTGGAGGAGT TTGCCGCGTG TGCGGATGAA GAAATCAAGG ATATCGAGTT CACTGTGACC 
ACGCTAAATT GCAATCAGCA ATCTCAGCAT GGCAGCGAGG ATAGCCACAC GCCGGAGCAC 
CAGCGAAGTG GCTCCAGATT GAGCATGAAG AAGACCGGAG CCCTGGGCAA GGCAGTTCGA 
TCGAAGCTAT CAAAGACTCT ATACAACAGC ATGGATGGCT CAAAGCCGAA GACCTCCTTG 
AAGCTCCTTG AGTCCCATGT CAGCGAGGAT ATGGATGACA AAAGTCCTAC GAGTCCTTCG 
CAGCCGCAGG CCTCCGGGAG CATGGGCCGC ATGTCCGCCT CGTCCTCCAC CTCATCTGCC 
GGCGGCCAGA TGGTGGACAA GTCCAAAAAG CGTTCCAAAC TGTGCGCGTT GTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6 ( NM_001300398.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287327.1 Drosophila melanogaster phosphodiesterase 6, transcript variant D (Pde6), mRNA.

Price & Availability↑

CloneID	OFa04872
Clone ID Related Accession (Same CDS sequence)	NM_142112.5 , NM_001300398.1
Accession Version	NM_001300398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 6, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATG TGTCATCGCC AGCTGGTGGC GCCGCATCTC CGGTTGAAAT GTCCACCACC 
AGCAGCTCTT CAGCCGCCAC TACGTCGGCG TCCTCGTCCA AACCGCTGAC CAATGGCGCC 
AACAAGACGG CTATATCAAC GGCAGCTGGA GGAGTGACAC CGGGTGCTGT TCCTGGACCC 
GGGTCCGGAG CAATCCCCGC CAGCAGTTCG AGTGGCAATC AGGTCAAACT GGAGCATCAT 
CACCGCCAGA GCAATAACAA TCGACCTGCG GTTACCAATC GCAGTTCGGA AACCAAGCTG 
ATGACACCTA CCGGCAGTTC CTCATCCCCA TCGCAATCTC CATCCCAAAC CCAAGCCAGT 
ATACAAACCC AAACGAGTCA GCAGGACAGG CTGGCCAAGG CGAGCACCAC GGCATCCCAG 
CAGGATGTCG ATGAGGTGGC CCGCCTTTTC GAGGAGAAGC CGGAAGCATT CGAGAAGTGG 
CTGACGGAAA GGGCTCCTCC AGAGGCACTC AGCCGACTGC AGGAGTTCAT TGAGAATCGC 
AAGCCGCACA AGCGACCCTC GGTCACCTCC GATCTCTTCC AGCAGTGGAT GGCCGCATCG 
CCCACCGTGC AGCAGAAATC GCCGAGGAGT CTCTCGAACT CTTCAGCATC GTCACTTCCG 
GAGTGCCGGA GGCATCTGAT GGACCTGGAC GAGGGCGAGC TGTTCATGGA ACTGATTCGC 
GACGTGGCCA ACGAGCTGGA CATCGATGTC CTCTGCCACA AGATTCTGGT CAATGTCGGA 
CTGCTCACCC ACGCGGATCG TGGCTCCCTG TTTCTGGCCA AGGGGACGCC CACCAACAAA 
TATCTGGTGG CCAAGCTATT CGATGTCACT CAAAAGACAG CACTCAAGGA CGCGGTGACT 
CGGGCCAGTG CCGAGGAGAT AATCATTCCC TTTGGCATTG GAATCGCGGG AATGGTGGCG 
CAGACAAAGC AGATGATTAA CATCAAGGAG GCCTACAAGG ATGCTCGCTT CAATTGCGAA 
ATCGATCTGA AGACCGGCTA CAAAACGAAC GCCATCCTCT GCATGCCCAT TTGCAATTAC 
GAGGGCGACA TCATAGGCGT GGCGCAAATT ATTAACAAAA CGAATGGCTG CATGGAGTTT 
GACGAGCACG ATGTGGAAAT TTTTCGCCGC TATTTGACCT TTTGTGGAAT TGGCATACAG 
AATGCCCAGC TCTTTGAGAT GTCCGTCCAG GAATACCGGC GTAATCAAAT TCTCCTCAAT 
TTGGCGCGCA GTATCTTCGA GGAGCAGAAT AATCTTGAGT GCTTAGTTAC CAAAATTATG 
ACAGAGGCCC GGGAACTACT CAAATGCGAA AGGTGCTCTG TGTTTCTAGT GGATCTCGAC 
TGTTGCGAGG CGAGCCATTT GGAGAAGATA ATTGAGAAGC CCAATCAGCC AGCCACCCGC 
GCCATCAAGT CCGCCGATAG CTTCGAGGAG AAGAAAATGC GCAATCGTTT CACGGTGCTC 
TTCGAACTCG GTGGAGAATA TCAGGCTGCC AATGTTTCGA GGCCATCTGT CAGCGAGTTG 
AGCTCCTCGA CGCTGGCCCA GATTGCTCAG TTTGTGGCCA CCACCGGGCA AACGGTGAAC 
ATCTGCGATG TCATCGAATG GGTCAGGGAT CACAATCAAA TTCGAGCGGA GGACGAAATA 
GATAGCACCC AGGCCATCCT CTGCATGCCC ATTATGAATG CACAGAAGAA GGTCATTGGC 
GTTGCCCAGC TTATCAACAA GGCCAACGGA GTTCCCTTCA CCGATTCGGA TGCCTCTATT 
TTCGAGGCAT TTGCCATTTT CTGTGGCTTG GGCATTCACA ACACCCAGAT GTACGAGAAT 
GCCTGCAAGT TGATGGCCAA GCAGAAGGTG GCCCTCGAGT GCCTCAGTTA CCATGCCACC 
GCTAGTCAGG ATCAGACGGA GAAGCTCACC CAGGACGTGA TTGCCGAGGC GGAGTCCTAC 
AACCTGTACA GCTTCACCTT TACGGACTTT GAGCTGGTCG ACGATGACAC CTGTCGCGCA 
GTGCTTCGCA TGTTTATGCA GTGCAATTTG GTCTCGCAGT TTCAGATACC ATATGATGTA 
CTTTGCCGCT GGGTTTTAAG TGTTCGCAAG AATTATCGTC CTGTTAAATA TCACAATTGG 
AGGCATGCTC TAAACGTGGC CCAAACGATG TTCGCCATGC TTAAAACTGG AAAGATGGAG 
CGCTTTATGA CAGATCTGGA GATTCTGGGT CTGTTGGTGG CCTGTTTGTG CCATGACTTG 
GATCATCGTG GCACCAACAA TGCATTCCAG ACCAAAACGG AGTCCCCGCT GGCCATTTTG 
TACACCACTA GCACGATGGA GCACCATCAC TTCGATCAGT GCGTAATGAT TCTCAATTCG 
GAGGGAAATA ATATATTTCA GGCCTTGTCA CCTGAGGACT ATCGTTCAGT CATGAAGACC 
GTGGAAAGCG CCATTCTGTC CACCGATCTG GCCATGTATT TCAAGAAAAG GAACGCTTTC 
CTAGAGTTGG TGGAAAATGG GGAATTCGAT TGGCAGGGCG AGGAGAAGAA AGATTTGCTT 
TGTGGCATGA TGATGACTGC TTGCGATGTG AGTGCCATTG CCAAGCCCTG GGAGGTTCAG 
CACAAGGTTG CCAAGTTGGT GGCCGATGAG TTCTTTGACC AAGGAGATCT CGAAAAGCTG 
CAGCTTAACA CCCAACCAGT CGCCATGATG GACAGGGAGC GCAAGGACGA GCTACCCAAG 
ATGCAGGTGG GCTTTATCGA CGTGATCTGT CTACCACTCT ACAGAGTGCT TTGCGACACC 
TTTCCCTGGA TCACTCCGCT CTATGAAGGA ACCTTGGAGA ACCGACGCAA CTGGCAGGAT 
CTGGCCGAGA AAGTGGAAAT GGGCCTCACA TGGATCGATC ACGATACGAT TGACAAACCC 
GTGGAGGAGT TTGCCGCGTG TGCGGATGAA GAAATCAAGG ATATCGAGTT CACTGTGACC 
ACGCTAAATT GCAATCAGCA ATCTCAGCAT GGCAGCGAGG ATAGCCACAC GCCGGAGCAC 
CAGCGAAGTG GCTCCAGATT GAGCATGAAG AAGACCGGAG CCCTGGGCAA GGCAGTTCGA 
TCGAAGCTAT CAAAGACTCT ATACAACAGC ATGGATGGCT CAAAGCCGAA GACCTCCTTG 
AAGCTCCTTG AGTCCCATGT CAGCGAGGAT ATGGATGACA AAAGTCCTAC GAGTCCTTCG 
CAGCCGCAGG CCTCCGGGAG CATGGGCCGC ATGTCCGCCT CGTCCTCCAC CTCATCTGCC 
GGCGGCCAGA TGGTGGACAA GTCCAAAAAG CGTTCCAAAC TGTGCGCGTT GTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287327.1
CDS	656..4012
Translation	MTDVSSPAGGAASPVEMSTTSSSSAATTSASSSKPLTNGANKTAISTAAGGVTPGAVPGPGSGAIPASSSSGNQVKLEHHHRQSNNNRPAVTNRSSETKLMTPTGSSSSPSQSPSQTQASIQTQTSQQDRLAKASTTASQQDVDEVARLFEEKPEAFEKWLTERAPPEALSRLQEFIENRKPHKRPSVTSDLFQQWMAASPTVQQKSPRSLSNSSASSLPECRRHLMDLDEGELFMELIRDVANELDIDVLCHKILVNVGLLTHADRGSLFLAKGTPTNKYLVAKLFDVTQKTALKDAVTRASAEEIIIPFGIGIAGMVAQTKQMINIKEAYKDARFNCEIDLKTGYKTNAILCMPICNYEGDIIGVAQIINKTNGCMEFDEHDVEIFRRYLTFCGIGIQNAQLFEMSVQEYRRNQILLNLARSIFEEQNNLECLVTKIMTEARELLKCERCSVFLVDLDCCEASHLEKIIEKPNQPATRAIKSADSFEEKKMRNRFTVLFELGGEYQAANVSRPSVSELSSSTLAQIAQFVATTGQTVNICDVIEWVRDHNQIRAEDEIDSTQAILCMPIMNAQKKVIGVAQLINKANGVPFTDSDASIFEAFAIFCGLGIHNTQMYENACKLMAKQKVALECLSYHATASQDQTEKLTQDVIAEAESYNLYSFTFTDFELVDDDTCRAVLRMFMQCNLVSQFQIPYDVLCRWVLSVRKNYRPVKYHNWRHALNVAQTMFAMLKTGKMERFMTDLEILGLLVACLCHDLDHRGTNNAFQTKTESPLAILYTTSTMEHHHFDQCVMILNSEGNNIFQALSPEDYRSVMKTVESAILSTDLAMYFKKRNAFLELVENGEFDWQGEEKKDLLCGMMMTACDVSAIAKPWEVQHKVAKLVADEFFDQGDLEKLQLNTQPVAMMDRERKDELPKMQVGFIDVICLPLYRVLCDTFPWITPLYEGTLENRRNWQDLAEKVEMGLTWIDHDTIDKPVEEFAACADEEIKDIEFTVTTLNCNQQSQHGSEDSHTPEHQRSGSRLSMKKTGALGKAVRSKLSKTLYNSMDGSKPKTSLKLLESHVSEDMDDKSPTSPSQPQASGSMGRMSASSSTSSAGGQMVDKSKKRSKLCALL

Target ORF information:

RefSeq Version	NM_001300398.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 6, transcript variant D (Pde6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300398.1

ORF Insert Sequence:

ATGACAGATG TGTCATCGCC AGCTGGTGGC GCCGCATCTC CGGTTGAAAT GTCCACCACC 
AGCAGCTCTT CAGCCGCCAC TACGTCGGCG TCCTCGTCCA AACCGCTGAC CAATGGCGCC 
AACAAGACGG CTATATCAAC GGCAGCTGGA GGAGTGACAC CGGGTGCTGT TCCTGGACCC 
GGGTCCGGAG CAATCCCCGC CAGCAGTTCG AGTGGCAATC AGGTCAAACT GGAGCATCAT 
CACCGCCAGA GCAATAACAA TCGACCTGCG GTTACCAATC GCAGTTCGGA AACCAAGCTG 
ATGACACCTA CCGGCAGTTC CTCATCCCCA TCGCAATCTC CATCCCAAAC CCAAGCCAGT 
ATACAAACCC AAACGAGTCA GCAGGACAGG CTGGCCAAGG CGAGCACCAC GGCATCCCAG 
CAGGATGTCG ATGAGGTGGC CCGCCTTTTC GAGGAGAAGC CGGAAGCATT CGAGAAGTGG 
CTGACGGAAA GGGCTCCTCC AGAGGCACTC AGCCGACTGC AGGAGTTCAT TGAGAATCGC 
AAGCCGCACA AGCGACCCTC GGTCACCTCC GATCTCTTCC AGCAGTGGAT GGCCGCATCG 
CCCACCGTGC AGCAGAAATC GCCGAGGAGT CTCTCGAACT CTTCAGCATC GTCACTTCCG 
GAGTGCCGGA GGCATCTGAT GGACCTGGAC GAGGGCGAGC TGTTCATGGA ACTGATTCGC 
GACGTGGCCA ACGAGCTGGA CATCGATGTC CTCTGCCACA AGATTCTGGT CAATGTCGGA 
CTGCTCACCC ACGCGGATCG TGGCTCCCTG TTTCTGGCCA AGGGGACGCC CACCAACAAA 
TATCTGGTGG CCAAGCTATT CGATGTCACT CAAAAGACAG CACTCAAGGA CGCGGTGACT 
CGGGCCAGTG CCGAGGAGAT AATCATTCCC TTTGGCATTG GAATCGCGGG AATGGTGGCG 
CAGACAAAGC AGATGATTAA CATCAAGGAG GCCTACAAGG ATGCTCGCTT CAATTGCGAA 
ATCGATCTGA AGACCGGCTA CAAAACGAAC GCCATCCTCT GCATGCCCAT TTGCAATTAC 
GAGGGCGACA TCATAGGCGT GGCGCAAATT ATTAACAAAA CGAATGGCTG CATGGAGTTT 
GACGAGCACG ATGTGGAAAT TTTTCGCCGC TATTTGACCT TTTGTGGAAT TGGCATACAG 
AATGCCCAGC TCTTTGAGAT GTCCGTCCAG GAATACCGGC GTAATCAAAT TCTCCTCAAT 
TTGGCGCGCA GTATCTTCGA GGAGCAGAAT AATCTTGAGT GCTTAGTTAC CAAAATTATG 
ACAGAGGCCC GGGAACTACT CAAATGCGAA AGGTGCTCTG TGTTTCTAGT GGATCTCGAC 
TGTTGCGAGG CGAGCCATTT GGAGAAGATA ATTGAGAAGC CCAATCAGCC AGCCACCCGC 
GCCATCAAGT CCGCCGATAG CTTCGAGGAG AAGAAAATGC GCAATCGTTT CACGGTGCTC 
TTCGAACTCG GTGGAGAATA TCAGGCTGCC AATGTTTCGA GGCCATCTGT CAGCGAGTTG 
AGCTCCTCGA CGCTGGCCCA GATTGCTCAG TTTGTGGCCA CCACCGGGCA AACGGTGAAC 
ATCTGCGATG TCATCGAATG GGTCAGGGAT CACAATCAAA TTCGAGCGGA GGACGAAATA 
GATAGCACCC AGGCCATCCT CTGCATGCCC ATTATGAATG CACAGAAGAA GGTCATTGGC 
GTTGCCCAGC TTATCAACAA GGCCAACGGA GTTCCCTTCA CCGATTCGGA TGCCTCTATT 
TTCGAGGCAT TTGCCATTTT CTGTGGCTTG GGCATTCACA ACACCCAGAT GTACGAGAAT 
GCCTGCAAGT TGATGGCCAA GCAGAAGGTG GCCCTCGAGT GCCTCAGTTA CCATGCCACC 
GCTAGTCAGG ATCAGACGGA GAAGCTCACC CAGGACGTGA TTGCCGAGGC GGAGTCCTAC 
AACCTGTACA GCTTCACCTT TACGGACTTT GAGCTGGTCG ACGATGACAC CTGTCGCGCA 
GTGCTTCGCA TGTTTATGCA GTGCAATTTG GTCTCGCAGT TTCAGATACC ATATGATGTA 
CTTTGCCGCT GGGTTTTAAG TGTTCGCAAG AATTATCGTC CTGTTAAATA TCACAATTGG 
AGGCATGCTC TAAACGTGGC CCAAACGATG TTCGCCATGC TTAAAACTGG AAAGATGGAG 
CGCTTTATGA CAGATCTGGA GATTCTGGGT CTGTTGGTGG CCTGTTTGTG CCATGACTTG 
GATCATCGTG GCACCAACAA TGCATTCCAG ACCAAAACGG AGTCCCCGCT GGCCATTTTG 
TACACCACTA GCACGATGGA GCACCATCAC TTCGATCAGT GCGTAATGAT TCTCAATTCG 
GAGGGAAATA ATATATTTCA GGCCTTGTCA CCTGAGGACT ATCGTTCAGT CATGAAGACC 
GTGGAAAGCG CCATTCTGTC CACCGATCTG GCCATGTATT TCAAGAAAAG GAACGCTTTC 
CTAGAGTTGG TGGAAAATGG GGAATTCGAT TGGCAGGGCG AGGAGAAGAA AGATTTGCTT 
TGTGGCATGA TGATGACTGC TTGCGATGTG AGTGCCATTG CCAAGCCCTG GGAGGTTCAG 
CACAAGGTTG CCAAGTTGGT GGCCGATGAG TTCTTTGACC AAGGAGATCT CGAAAAGCTG 
CAGCTTAACA CCCAACCAGT CGCCATGATG GACAGGGAGC GCAAGGACGA GCTACCCAAG 
ATGCAGGTGG GCTTTATCGA CGTGATCTGT CTACCACTCT ACAGAGTGCT TTGCGACACC 
TTTCCCTGGA TCACTCCGCT CTATGAAGGA ACCTTGGAGA ACCGACGCAA CTGGCAGGAT 
CTGGCCGAGA AAGTGGAAAT GGGCCTCACA TGGATCGATC ACGATACGAT TGACAAACCC 
GTGGAGGAGT TTGCCGCGTG TGCGGATGAA GAAATCAAGG ATATCGAGTT CACTGTGACC 
ACGCTAAATT GCAATCAGCA ATCTCAGCAT GGCAGCGAGG ATAGCCACAC GCCGGAGCAC 
CAGCGAAGTG GCTCCAGATT GAGCATGAAG AAGACCGGAG CCCTGGGCAA GGCAGTTCGA 
TCGAAGCTAT CAAAGACTCT ATACAACAGC ATGGATGGCT CAAAGCCGAA GACCTCCTTG 
AAGCTCCTTG AGTCCCATGT CAGCGAGGAT ATGGATGACA AAAGTCCTAC GAGTCCTTCG 
CAGCCGCAGG CCTCCGGGAG CATGGGCCGC ATGTCCGCCT CGTCCTCCAC CTCATCTGCC 
GGCGGCCAGA TGGTGGACAA GTCCAAAAAG CGTTCCAAAC TGTGCGCGTT GTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6 ( NM_001275637.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262566.1 Drosophila melanogaster phosphodiesterase 6, transcript variant C (Pde6), mRNA.

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CloneID	OFa07987
Clone ID Related Accession (Same CDS sequence)	NM_001275637.2
Accession Version	NM_001275637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 6, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275637.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATG TGTCATCGCC AGCTGGTGGC GCCGCATCTC CGGTTGAAAT GTCCACCACC 
AGCAGCTCTT CAGCCGCCAC TACGTCGGCG TCCTCGTCCA AACCGCTGAC CAATGGCGCC 
AACAAGACGG CTATATCAAC GGCAGCTGGA GGAGTGACAC CGGGTGCTGT TCCTGGACCC 
GGGTCCGGAG CAATCCCCGC CAGCAGTTCG AGTGGCAATC AGGTCAAACT GGAGCATCAT 
CACCGCCAGA GCAATAACAA TCGACCTGCG GTTACCAATC GCAGTTCGGA AACCAAGCTG 
ATGACACCTA CCGGCAGTTC CTCATCCCCA TCGCAATCTC CATCCCAAAC CCAAGCCAGT 
ATACAAACCC AAACGAGTCA GCAGGACAGG CTGGCCAAGG CGAGCACCAC GGCATCCCAG 
CAGGATGTCG ATGAGGTGGC CCGCCTTTTC GAGGAGAAGC CGGAAGCATT CGAGAAGTGG 
CTGACGGAAA GGGCTCCTCC AGAGGCACTC AGCCGACTGC AGGAGTTCAT TGAGAATCGC 
AAGCCGCACA AGCGACCCTC GGTCACCTCC GATCTCTTCC AGCAGTGGAT GGCCGCATCG 
CCCACCGTGC AGCAGAAATC GCCGAGGAGT CTCTCGAACT CTTCAGCATC GTCACTTCCG 
GAGTGCCGGA GGCATCTGAT GGACCTGGAC GAGGGCGAGC TGTTCATGGA ACTGATTCGC 
GACGTGGCCA ACGAGCTGGA CATCGATGTC CTCTGCCACA AGATTCTGGT CAATGTCGGA 
CTGCTCACCC ACGCGGATCG TGGCTCCCTG TTTCTGGCCA AGGGGACGCC CACCAACAAA 
TATCTGGTGG CCAAGCTATT CGATGTCACT CAAAAGACAG CACTCAAGGA CGCGGTGACT 
CGGGCCAGTG CCGAGGAGAT AATCATTCCC TTTGGCATTG GAATCGCGGG AATGGTGGCG 
CAGACAAAGC AGATGATTAA CATCAAGGAG GCCTACAAGG ATGCTCGCTT CAATTGCGAA 
ATCGATCTGA AGACCGGCTA CAAAACGAAC GCCATCCTCT GCATGCCCAT TTGCAATTAC 
GAGGGCGACA TCATAGGCGT GGCGCAAATT ATTAACAAAA CGAATGGCTG CATGGAGTTT 
GACGAGCACG ATGTGGAAAT TTTTCGCCGC TATTTGACCT TTTGTGGAAT TGGCATACAG 
AATGCCCAGC TCTTTGAGAT GTCCGTCCAG GAATACCGGC GTAATCAAAT TCTCCTCAAT 
TTGGCGCGCA GTATCTTCGA GGAGCAGAAT AATCTTGAGT GCTTAGTTAC CAAAATTATG 
ACAGAGGCCC GGGAACTACT CAAATGCGAA AGGTGCTCTG TGTTTCTAGT GGATCTCGAC 
TGTTGCGAGG CGAGCCATTT GGAGAAGATA ATTGAGAAGC CCAATCAGCC AGCCACCCGC 
GCCATCAAGT CCGCCGATAG CTTCGAGGAG AAGAAAATGC GCAATCGTTT CACGGTGCTC 
TTCGAACTCG GTGGAGAATA TCAGGCTGCC AATGTTTCGA GGCCATCTGT CAGCGAGTTG 
AGCTCCTCGA CGCTGGCCCA GATTGCTCAG TTTGTGGCCA CCACCGGGCA AACGGTGAAC 
ATCTGCGATG TCATCGAATG GGTCAGGGAT CACAATCAAA TTCGAGCGGA GGACGAAATA 
GATAGCACCC AGGCCATCCT CTGCATGCCC ATTATGAATG CACAGAAGAA GGTCATTGGC 
GTTGCCCAGC TTATCAACAA GGCCAACGGA GTTCCCTTCA CCGATTCGGA TGCCTCTATT 
TTCGAGGCAT TTGCCATTTT CTGTGGCTTG GGCATTCACA ACACCCAGAT GTACGAGAAT 
GCCTGCAAGT TGATGGCCAA GCAGAAGGTG GCCCTCGAGT GCCTCAGTTA CCATGCCACC 
GCTAGTCAGG ATCAGACGGA GAAGCTCACC CAGGACGTGA TTGCCGAGGC GGAGTCCTAC 
AACCTGTACA GCTTCACCTT TACGGACTTT GAGCTGGTCG ACGATGACAC CTGTCGCGCA 
GTGCTTCGCA TGTTTATGCA GTGCAATTTG GTCTCGCAGT TTCAGATACC ATATGATGTA 
CTTTGCCGCT GGGTTTTAAG TGTTCGCAAG AATTATCGTC CTGTTAAATA TCACAATTGG 
AGGCATGCTC TAAACGTGGC CCAAACGATG TTCGCCATGC TTAAAACTGG AAAGATGGAG 
CGCTTTATGA CAGATCTGGA GATTCTGGGT CTGTTGGTGG CCTGTTTGTG CCATGACTTG 
GATCATCGTG GCACCAACAA TGCATTCCAG ACCAAAACGG AGTCCCCGCT GGCCATTTTG 
TACACCACTA GCACGATGGA GCACCATCAC TTCGATCAGT GCGTAATGAT TCTCAATTCG 
GAGGGAAATA ATATATTTCA GGCCTTGTCA CCTGAGGACT ATCGTTCAGT CATGAAGACC 
GTGGAAAGCG CCATTCTGTC CACCGATCTG GCCATGTATT TCAAGAAAAG GAACGCTTTC 
CTAGAGTTGG TGGAAAATGG GGAATTCGAT TGGCAGGGCG AGGAGAAGAA AGATTTGCTT 
TGTGGCATGA TGATGACTGC TTGCGATGTG AGTGCCATTG CCAAGCCCTG GGAGGTTCAG 
CACAAGGTTG CCAAGTTGGT GGCCGATGAG TTCTTTGACC AAGGAGATCT CGAAAAGCTG 
CAGCTTAACA CCCAACCAGT CGCCATGATG GACAGGGAGC GCAAGGACGA GCTACCCAAG 
ATGCAGGTGG GCTTTATCGA CGTGATCTGT CTACCACTCT ACAGAGTGCT TTGCGACACC 
TTTCCCTGGA TCACTCCGCT CTATGAAGGA ACCTTGGAGA ACCGACGCAA CTGGCAGGAT 
CTGGCCGAGA AAGTGGAAAT GGGCCTCACA TGGATCGATC ACGATACGAT TGACAAACCC 
GTGGAGGAGT TTGCCGCGTG TGCGGATGAA GAAATCAAGG ATATCGAGTT CACTGTGACC 
ACGCTAAATT GCAATCAGCA ATCTCAGCAT GGCAGCGAGG ATAGCCACAC GCCGGAGCAC 
CAGCGAAGTG GCTCCAGATT GAGCATGAAG AAGACCGGAG CCCTGGGCAA GGCAGTTCGA 
TCGAAGCTAT CAAAGACTCT ATACAACAGC ATGGATGGCT CAAAGCCGAA GACCTCCTTG 
AAGCTCCTTG AGTCCCATGT CAGCGAGGAT ATGGATGACA AAAGTCCTAC GAGTCCTTCG 
CAGCCGCAGG CCTCCGGGAG CATGGGCCGC ATGTCCGCCT CGTCCTCCAC CTCATCTGCC 
GGCGGCCAGA TGGTGGACAA GTCCAAAAAG CGTTCCAAAC TGTGCGCGTT GTTATGACGA 
AAGCCGTCCA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262566.1
CDS	1015..4389
Translation	MTDVSSPAGGAASPVEMSTTSSSSAATTSASSSKPLTNGANKTAISTAAGGVTPGAVPGPGSGAIPASSSSGNQVKLEHHHRQSNNNRPAVTNRSSETKLMTPTGSSSSPSQSPSQTQASIQTQTSQQDRLAKASTTASQQDVDEVARLFEEKPEAFEKWLTERAPPEALSRLQEFIENRKPHKRPSVTSDLFQQWMAASPTVQQKSPRSLSNSSASSLPECRRHLMDLDEGELFMELIRDVANELDIDVLCHKILVNVGLLTHADRGSLFLAKGTPTNKYLVAKLFDVTQKTALKDAVTRASAEEIIIPFGIGIAGMVAQTKQMINIKEAYKDARFNCEIDLKTGYKTNAILCMPICNYEGDIIGVAQIINKTNGCMEFDEHDVEIFRRYLTFCGIGIQNAQLFEMSVQEYRRNQILLNLARSIFEEQNNLECLVTKIMTEARELLKCERCSVFLVDLDCCEASHLEKIIEKPNQPATRAIKSADSFEEKKMRNRFTVLFELGGEYQAANVSRPSVSELSSSTLAQIAQFVATTGQTVNICDVIEWVRDHNQIRAEDEIDSTQAILCMPIMNAQKKVIGVAQLINKANGVPFTDSDASIFEAFAIFCGLGIHNTQMYENACKLMAKQKVALECLSYHATASQDQTEKLTQDVIAEAESYNLYSFTFTDFELVDDDTCRAVLRMFMQCNLVSQFQIPYDVLCRWVLSVRKNYRPVKYHNWRHALNVAQTMFAMLKTGKMERFMTDLEILGLLVACLCHDLDHRGTNNAFQTKTESPLAILYTTSTMEHHHFDQCVMILNSEGNNIFQALSPEDYRSVMKTVESAILSTDLAMYFKKRNAFLELVENGEFDWQGEEKKDLLCGMMMTACDVSAIAKPWEVQHKVAKLVADEFFDQGDLEKLQLNTQPVAMMDRERKDELPKMQVGFIDVICLPLYRVLCDTFPWITPLYEGTLENRRNWQDLAEKVEMGLTWIDHDTIDKPVEEFAACADEEIKDIEFTVTTLNCNQQSQHGSEDSHTPEHQRSGSRLSMKKTGALGKAVRSKLSKTLYNSMDGSKPKTSLKLLESHVSEDMDDKSPTSPSQPQASGSMGRMSASSSTSSAGGQMVDKSKKRSKLCALL*RKPSN

Target ORF information:

RefSeq Version	NM_001275637.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 6, transcript variant C (Pde6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275637.2

ORF Insert Sequence:

ATGACAGATG TGTCATCGCC AGCTGGTGGC GCCGCATCTC CGGTTGAAAT GTCCACCACC 
AGCAGCTCTT CAGCCGCCAC TACGTCGGCG TCCTCGTCCA AACCGCTGAC CAATGGCGCC 
AACAAGACGG CTATATCAAC GGCAGCTGGA GGAGTGACAC CGGGTGCTGT TCCTGGACCC 
GGGTCCGGAG CAATCCCCGC CAGCAGTTCG AGTGGCAATC AGGTCAAACT GGAGCATCAT 
CACCGCCAGA GCAATAACAA TCGACCTGCG GTTACCAATC GCAGTTCGGA AACCAAGCTG 
ATGACACCTA CCGGCAGTTC CTCATCCCCA TCGCAATCTC CATCCCAAAC CCAAGCCAGT 
ATACAAACCC AAACGAGTCA GCAGGACAGG CTGGCCAAGG CGAGCACCAC GGCATCCCAG 
CAGGATGTCG ATGAGGTGGC CCGCCTTTTC GAGGAGAAGC CGGAAGCATT CGAGAAGTGG 
CTGACGGAAA GGGCTCCTCC AGAGGCACTC AGCCGACTGC AGGAGTTCAT TGAGAATCGC 
AAGCCGCACA AGCGACCCTC GGTCACCTCC GATCTCTTCC AGCAGTGGAT GGCCGCATCG 
CCCACCGTGC AGCAGAAATC GCCGAGGAGT CTCTCGAACT CTTCAGCATC GTCACTTCCG 
GAGTGCCGGA GGCATCTGAT GGACCTGGAC GAGGGCGAGC TGTTCATGGA ACTGATTCGC 
GACGTGGCCA ACGAGCTGGA CATCGATGTC CTCTGCCACA AGATTCTGGT CAATGTCGGA 
CTGCTCACCC ACGCGGATCG TGGCTCCCTG TTTCTGGCCA AGGGGACGCC CACCAACAAA 
TATCTGGTGG CCAAGCTATT CGATGTCACT CAAAAGACAG CACTCAAGGA CGCGGTGACT 
CGGGCCAGTG CCGAGGAGAT AATCATTCCC TTTGGCATTG GAATCGCGGG AATGGTGGCG 
CAGACAAAGC AGATGATTAA CATCAAGGAG GCCTACAAGG ATGCTCGCTT CAATTGCGAA 
ATCGATCTGA AGACCGGCTA CAAAACGAAC GCCATCCTCT GCATGCCCAT TTGCAATTAC 
GAGGGCGACA TCATAGGCGT GGCGCAAATT ATTAACAAAA CGAATGGCTG CATGGAGTTT 
GACGAGCACG ATGTGGAAAT TTTTCGCCGC TATTTGACCT TTTGTGGAAT TGGCATACAG 
AATGCCCAGC TCTTTGAGAT GTCCGTCCAG GAATACCGGC GTAATCAAAT TCTCCTCAAT 
TTGGCGCGCA GTATCTTCGA GGAGCAGAAT AATCTTGAGT GCTTAGTTAC CAAAATTATG 
ACAGAGGCCC GGGAACTACT CAAATGCGAA AGGTGCTCTG TGTTTCTAGT GGATCTCGAC 
TGTTGCGAGG CGAGCCATTT GGAGAAGATA ATTGAGAAGC CCAATCAGCC AGCCACCCGC 
GCCATCAAGT CCGCCGATAG CTTCGAGGAG AAGAAAATGC GCAATCGTTT CACGGTGCTC 
TTCGAACTCG GTGGAGAATA TCAGGCTGCC AATGTTTCGA GGCCATCTGT CAGCGAGTTG 
AGCTCCTCGA CGCTGGCCCA GATTGCTCAG TTTGTGGCCA CCACCGGGCA AACGGTGAAC 
ATCTGCGATG TCATCGAATG GGTCAGGGAT CACAATCAAA TTCGAGCGGA GGACGAAATA 
GATAGCACCC AGGCCATCCT CTGCATGCCC ATTATGAATG CACAGAAGAA GGTCATTGGC 
GTTGCCCAGC TTATCAACAA GGCCAACGGA GTTCCCTTCA CCGATTCGGA TGCCTCTATT 
TTCGAGGCAT TTGCCATTTT CTGTGGCTTG GGCATTCACA ACACCCAGAT GTACGAGAAT 
GCCTGCAAGT TGATGGCCAA GCAGAAGGTG GCCCTCGAGT GCCTCAGTTA CCATGCCACC 
GCTAGTCAGG ATCAGACGGA GAAGCTCACC CAGGACGTGA TTGCCGAGGC GGAGTCCTAC 
AACCTGTACA GCTTCACCTT TACGGACTTT GAGCTGGTCG ACGATGACAC CTGTCGCGCA 
GTGCTTCGCA TGTTTATGCA GTGCAATTTG GTCTCGCAGT TTCAGATACC ATATGATGTA 
CTTTGCCGCT GGGTTTTAAG TGTTCGCAAG AATTATCGTC CTGTTAAATA TCACAATTGG 
AGGCATGCTC TAAACGTGGC CCAAACGATG TTCGCCATGC TTAAAACTGG AAAGATGGAG 
CGCTTTATGA CAGATCTGGA GATTCTGGGT CTGTTGGTGG CCTGTTTGTG CCATGACTTG 
GATCATCGTG GCACCAACAA TGCATTCCAG ACCAAAACGG AGTCCCCGCT GGCCATTTTG 
TACACCACTA GCACGATGGA GCACCATCAC TTCGATCAGT GCGTAATGAT TCTCAATTCG 
GAGGGAAATA ATATATTTCA GGCCTTGTCA CCTGAGGACT ATCGTTCAGT CATGAAGACC 
GTGGAAAGCG CCATTCTGTC CACCGATCTG GCCATGTATT TCAAGAAAAG GAACGCTTTC 
CTAGAGTTGG TGGAAAATGG GGAATTCGAT TGGCAGGGCG AGGAGAAGAA AGATTTGCTT 
TGTGGCATGA TGATGACTGC TTGCGATGTG AGTGCCATTG CCAAGCCCTG GGAGGTTCAG 
CACAAGGTTG CCAAGTTGGT GGCCGATGAG TTCTTTGACC AAGGAGATCT CGAAAAGCTG 
CAGCTTAACA CCCAACCAGT CGCCATGATG GACAGGGAGC GCAAGGACGA GCTACCCAAG 
ATGCAGGTGG GCTTTATCGA CGTGATCTGT CTACCACTCT ACAGAGTGCT TTGCGACACC 
TTTCCCTGGA TCACTCCGCT CTATGAAGGA ACCTTGGAGA ACCGACGCAA CTGGCAGGAT 
CTGGCCGAGA AAGTGGAAAT GGGCCTCACA TGGATCGATC ACGATACGAT TGACAAACCC 
GTGGAGGAGT TTGCCGCGTG TGCGGATGAA GAAATCAAGG ATATCGAGTT CACTGTGACC 
ACGCTAAATT GCAATCAGCA ATCTCAGCAT GGCAGCGAGG ATAGCCACAC GCCGGAGCAC 
CAGCGAAGTG GCTCCAGATT GAGCATGAAG AAGACCGGAG CCCTGGGCAA GGCAGTTCGA 
TCGAAGCTAT CAAAGACTCT ATACAACAGC ATGGATGGCT CAAAGCCGAA GACCTCCTTG 
AAGCTCCTTG AGTCCCATGT CAGCGAGGAT ATGGATGACA AAAGTCCTAC GAGTCCTTCG 
CAGCCGCAGG CCTCCGGGAG CATGGGCCGC ATGTCCGCCT CGTCCTCCAC CTCATCTGCC 
GGCGGCCAGA TGGTGGACAA GTCCAAAAAG CGTTCCAAAC TGTGCGCGTT GTTATGACGA 
AAGCCGTCCA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pde6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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