Usp47 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp47
Entrez Gene ID	38644
Full Name	Ubiquitin specific protease 47
Synonyms	BcDNA:LD12764,CG5486,D-UBP-64E,D-Ubp-64E,Dmel\CG5486,E(var)1,E(var)3-64E,E-var(3)64E,Q24574,UBP,UBP64E,Ubp64,Ubp64E,Upd64E,dUSP47,dUbp64,ubp64,ubp64E,usp47
General protein information	Preferred Names Ubiquitin specific protease 47 Names CG5486 gene product from transcript CG5486-RD CG5486-PA CG5486-PB CG5486-PD Ubiquitin-specific protease 64E Usp47-PA Usp47-PB Usp47-PD ubiquitin specific protease 47
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64E5-64E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_168129.4	NP_729087.1	ubiquitin specific protease 47, isoform A
NM_079213.4	NP_523937.2	ubiquitin specific protease 47, isoform B
NM_206279.4	NP_996001.2	ubiquitin specific protease 47, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP47	NP_001269588.1
Mus musculus (house mouse)	Usp47	NP_796223.2
Pan troglodytes (chimpanzee)	USP47	XP_001171097.1
Rattus norvegicus (Norway rat)	Usp47	NP_001101012.2
Gallus gallus (chicken)	USP47	NP_001186511.1
Drosophila melanogaster (fruit fly)	Ubp64E	NP_996001.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007106	XP_001237119.2
Xenopus tropicalis (tropical clawed frog)	usp47	NP_001090710.1
Canis lupus familiaris (dog)	USP47	XP_005633729.1
Bos taurus (cattle)	USP47	NP_001230219.1
	USP47	XP_001098399.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the presence of USP47, MAPK would be stabilized as USP47 acts to deubiquitinate MAPK, thus counteracting the direct ubiquitination of MAPK by POE/UBR4 activity.
Title: The Deubiquitinase USP47 Stabilizes MAPK by Counteracting the Function of the N-end Rule ligase POE/UBR4 in Drosophila.

The following Usp47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17617	NM_206279.4 Latest version!	Drosophila melanogaster ubiquitin specific protease 47, transcript variant D (Usp47), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa17618	NM_079213.4 Latest version!	Drosophila melanogaster ubiquitin specific protease 47, transcript variant B (Usp47), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa17618	NM_168129.4 Latest version!	Drosophila melanogaster ubiquitin specific protease 47, transcript variant A (Usp47), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp47 ( NM_206279.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996001.2 Drosophila melanogaster ubiquitin specific protease 47, transcript variant D (Usp47), mRNA.

Price & Availability↑

CloneID	OFa17617
Clone ID Related Accession (Same CDS sequence)	NM_206279.4
Accession Version	NM_206279.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 47, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206279.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGACA AGGAGAGCGA GCAGTGCACC GTCTCGGTCT TTGACCAGAC ACCCGGCTCG 
GAGCAAAAGA AGATCAACGT AGTGGTCCGC TCCCACTTCA CGGTGAAACG CGTGATTGAC 
CTTATTGGCA CGCAGTTCTC GTACGAAAAG TTCGAGCTGT TGCTGCAGCC ACACGATAAC 
AAGGATCTGG TCAATCTGAA TGCCCTGGAA TCTCAGCTGA TGTACGAAGT GGCTGGTTTT 
GAGCCCCAGC TGAAGAATCA CTTGATCCTG CTGCCCTCCG GCAGTTGGGA TGGTGACGTG 
ACCAAACGCT TTGAGTTACC CATTAAAAGA GTGGTAGTCA AAAAGGTTAT GAAGTCGGAC 
GGCGAGAAGG CCAAGAGTCC TGCCACCGGC GAGAAGAAGA AACGCGTAGT TGGTGAGAAA 
ACGAAAAAGA AGCCAGCTTC GGGATCTTCC TCTCCAAGCA AGGCCAAGAC GACGAGTGAG 
GACTCTTTGG CTAAGACCAG CATCAGTTCG GAATCAAGTC CCGAAAAGAC TTCCAAGATC 
AAGACGACTG CAGCAAAGAT CTCCAAGCCC GGTTCGGAAA AAGCTCCCAG GGCTAGCCCT 
GAAGAATGTC CCGAGCTGTC TACAGAGATA AACTCTAAAA ACACTTCTTC GGAGAGTCCT 
GTGGCCAAGA AAACGGCCAA GGTCACTTCT AAGCCGACTT TAGAGTTGCT GAGTCCAATC 
AAGCCGTCAT CACCCATCAA AGAGTTGGAC TGCGAACCAG TAGACACCCT GAGCAAGCAA 
CAGCTCTCTG AGCAACTGCA GCTGTATCCA CAAGATGCTG AACAATTGTC GGATGACGAC 
CTGGCACTCG GAGCATCTGC TAGTCCGACA ATGCTGGGAC CTGGATACGA TTACGGAGCT 
CCGACCGGTG ACTCGGACGT CGAGGGTGTG ACCGGAGTGA CAGACCCATC CACAATTGGA 
ACAGACGATG GTACGTACCC GGCTCTGTCA AACTTCTATC GTCGCAAGTA TGGAGGCGAT 
GAGTTGCGAG CATGGCAGAG AGTCAACACA ACGGGTGCGG ACTTCGTGTC CTCGGCCACC 
ACAGAAACTG AGGCAGAGGC AAGGCAGGCG AGCTTGGGTC CCAGGGGATA TGTTGGTTTG 
GTCAACCAAG CCATGACCTG CTATCTAAAC AGCTTGCTGC AGGCACTCTT TATGACACCC 
GAGTTCAGAA ATGCTCTGTA CCGCTGGGAG TTCGACAATG ACAACGAGGC CAAGAACATA 
CCATACCAAC TGCAAAAGCT CTTCCTCAAC TTGCAGACCT CGCCCAAAGC GGCGGTAGAA 
ACCACAGACC TGACTCGCAG CTTTGGGTGG GACTCGACGG AGGCATGGCA GCAGCACGAT 
ATCCAGGAAC TGTGCCGAGT TATGTTCGAC GCTCTGGAGC ACAAATTCAA GAACACTAAG 
CAGGCAAATC TCATCTCTAA TCTGTACGAG GGCAAGATGA ATGATTATGT CAAGTGTTTG 
GAGTGCAATA CCGAGAAGAC GCGCGAGGAC ACCTTTCTAG ATATCCCGCT CCCTGTGAGG 
CCCTTTGGAA GCAGCTCCGC ATACGGCAGC ATCGAGGAAG CTCTGCGTGC CTTCGTTCAG 
CCCGAAACAC TCGATGGCAA TAACCAGTAT CTGTGCGAGA AGTGCAAGAA AAAATGCGAC 
GCCCACAAGG GACTGCACTT TAAGTCCTTT CCCTACATCC TCACGCTGCA CCTTAAACGC 
TTTGACTTTG ACTACCAGAC CATGCACCGC ATCAAATTAA ACGACAGAGT GACCTTCCCT 
CAGACGCTCA ACCTGAACAC GTTCATTAAC CGAAGTGGAA ACAGCGGTGA GCAAAACTCT 
CAGCTCAACG GCACCGTGGA CGATTGCAGC ACGGCGGATA GTGGATCCGC CATGGAGGAC 
GATAATTTGA GCAGCGGCGT GGTGACCACA GCGAGCTCTA GTCAGCACGA AAACGATCTG 
AACGACGAGG ATGAAGGCAT CGACATGAGC AGCAGCACCA GCAAGAGCGC CAAGCAAGGA 
TCCGGTCCGT ATCTCTACGA ACTGTTCGCC ATCATGATTC ATTCAGGCAG CGCTTCGGGC 
GGACACTACT ATGCTTATAT TAAGGACTTC GACAACAACG AGTGGTTCTG TTTTAACGAT 
CAGAACGTGA CTAGTATCAC CCAAGAGGAC ATCCAACGTT CGTTTGGCGG ACCCAACGGG 
AGCTACTACT CCAGTGCCTA CACCTCCAGC ACCAATGCAT ACATGTTGAT GTACCGGCAG 
GTGGATGCCA AAAGAAACGA GCTAGTCGCC AAGGTGGCCG ACTTTCCTGA GCACATCAAA 
ACGCTGCTAC CAAAGCTGCA CTCGGAGGAG GAGACGCGCG TGTCCCGCCT GGGCAGGCAT 
ATCACGGTTA CGGACTTGGC TCTTCCGGAT TTGTACAAGC CGCGCGTCTA CTTCTATAAT 
CCCTCCCTGA AGAAGATGAA GATTACCAGA GTGTACGTAT CGCAGAGTTT CAACATCAAC 
TTGGTCCTCA TGTCGGCCTA TGAGATGTTG AATGTGGAAC AATTCGCGCC GCTTTCCCGC 
TGTCGTTTGG TGGCTTACAA CTCCTCGATG GACACGATTA TTCAGTCCCT GGAGAGTTGC 
ACAGACCCAG CTTTGACCGA ACTGCGCGCG GCGCAGAACT ACAGTCTCGA CTTCCTGCTG 
GAGTACCGGG CCGAGGACCA AGAGTTTGAG GTCTATCCGC CGAACGGCAT TACTTGGTAT 
GTGTTTAAGG TAGATCTATC GACCATGGCG ATGGACGGTC CCTTCCTGGT TTACTCAGCA 
GCGCGGGAGC GGGAAGCCAG CGACGTCCTT CGCCGCTCGA TCGCCCTCCG TTTGCACATT 
AGCGAGCAGC AGTTCCTACT CGCCACGGTG CGCGCCACGG TGCCGAAGGC TTTCGTGTCC 
TACGATCCGC ATCCGACGCC GGAGGCCCTG CAACATCTCC AGAACATGGC CAACACCCAG 
TTTAAGTCCA TAACATACTT CTACTTGAAC GTACCCAACA CGGATGCCGC GACCCTTGAG 
ATGCTCGGTG TGCCCACCGT TGAGTCAGTC GAGTGCGCCA GCGGGGGTGA TGTTGTGGAT 
GCTGCAATGA TGAATGGCGT GGCACCTGGT CACATGTCAT CCAGCAACGA CTACGATTGG 
AGGCGCTACA AGCGAGATCT AGTGGAACCC ATGTCACAAC CCAGTCCCAG CCACGGCCAC 
GAGTCCAACT CGGAGGACAG TAGTCTGAGC GACGGAGACC GAACTCTGGT GGAGACGGAC 
AACATGGCAC ATCGCGGTGG AGGCGACAGC CAAGTCAGTT CCACCAGTCA CTCGCCGCAG 
CTGTCTAGTC CGGAGGACGA AGCAGCCTCA CACGATGCCA TGATGCGCGT TCATGCCTAC 
TGCAATGGAA ACGGCAGTTA TGCGGCGGCC GATGTGGTGG ATCCGCTGCT CCTGCCCACC 
AGCACCAACC ACTTCTTCTA CGCCACAAAG GTCGAGTGCG TGGATGTGGT TGGCACCGGT 
TCCAGTTCTG GTCACCAGTC CGACGAAGAG GCTCAGCTGC GTAAGCCCAC GCGTGCCTAT 
AAGCTGCTGG TGGGCACACA CATGCGAATG GGGGCCTTTA AGAAACACAT CGAGCAGCTG 
ATCCAAGTGC CCGCCGCCCA CTTTAAACTG CAGCGGAAAC ACGATAATAA TCTGTCGAAC 
AACCAGAACA ACTCGTTGGT ACATCTCATA GAAGGCGAAA CACTGACGGT GGAGTTGGGT 
AAGACGCTGG AGCCGGACGA GTTCAAGGCC AAGATTCACT TCCTGCGACT GGCGGACATT 
GATAACGAGA CGTCCAAACT GCCTTGCGTA TGCGAGTGGG TCTACAATGC CAACACCACG 
GCGGAGCAGG CCAAGAAGGA ACTGGTGGCC AAGCTGCATC GCATAGACGC CAAGTACGCC 
ACACTGTCTG TGCAAAACTG TCGCATCTGG CTGAAGGGCG GACGTATTCC CATCAAAATT 
CTGTCCGACG ATGAAACGCT CTACTGCGAC ATGCGCTCCA GCATCGCAGC GGAGTTCATT 
GTGCAAGAGT GCGAGGAGGA AGTGGACCCG CAGCCCAAGG ACGATTCTCT GACTTTATTT 
GTAAGACGGT GGTGTCCGGC TAAACTGGAG TTTGGAAAGT TCCAAGAAAT CACTTTGGAC 
CAGGACAGCG AAATCAGACT TTCATTATCA CAGATCAGCG ACATCCCAAT AGACAAACTG 
AGCTATATGA AGTTGAACAG CAACTTCCCC TGCACAAGCA TATCAGCGTT AAGTGTTAAC 
GAGTCCAGCA GTTGGTACTC CGTGCCCACC ACCCTGGACA AATATCCGCT TAATAGCACA 
CAGACAGGAA ACATCTACCT GTACAAAGAT AGGACGGTAC CTGCGCGAGA GCTGACGCTG 
GAGGAGCGAC GCCTGATGAA CGCCAGGGAG AAGGCACGAC TGGATCGGGT GGGCTGCGTG 
TCCACCACGC GGTATGCGCA GCGCCGGGAA CGTGCCTTGA AGATTTACCT GGACTCTCCG 
GAGAAGTCGA GCAACGTGAC CGCCTCCGCA CCGATGGACG TGCATGTCAA TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996001.2
CDS	375..4991
Translation	MTDKESEQCTVSVFDQTPGSEQKKINVVVRSHFTVKRVIDLIGTQFSYEKFELLLQPHDNKDLVNLNALESQLMYEVAGFEPQLKNHLILLPSGSWDGDVTKRFELPIKRVVVKKVMKSDGEKAKSPATGEKKKRVVGEKTKKKPASGSSSPSKAKTTSEDSLAKTSISSESSPEKTSKIKTTAAKISKPGSEKAPRASPEECPELSTEINSKNTSSESPVAKKTAKVTSKPTLELLSPIKPSSPIKELDCEPVDTLSKQQLSEQLQLYPQDAEQLSDDDLALGASASPTMLGPGYDYGAPTGDSDVEGVTGVTDPSTIGTDDGTYPALSNFYRRKYGGDELRAWQRVNTTGADFVSSATTETEAEARQASLGPRGYVGLVNQAMTCYLNSLLQALFMTPEFRNALYRWEFDNDNEAKNIPYQLQKLFLNLQTSPKAAVETTDLTRSFGWDSTEAWQQHDIQELCRVMFDALEHKFKNTKQANLISNLYEGKMNDYVKCLECNTEKTREDTFLDIPLPVRPFGSSSAYGSIEEALRAFVQPETLDGNNQYLCEKCKKKCDAHKGLHFKSFPYILTLHLKRFDFDYQTMHRIKLNDRVTFPQTLNLNTFINRSGNSGEQNSQLNGTVDDCSTADSGSAMEDDNLSSGVVTTASSSQHENDLNDEDEGIDMSSSTSKSAKQGSGPYLYELFAIMIHSGSASGGHYYAYIKDFDNNEWFCFNDQNVTSITQEDIQRSFGGPNGSYYSSAYTSSTNAYMLMYRQVDAKRNELVAKVADFPEHIKTLLPKLHSEEETRVSRLGRHITVTDLALPDLYKPRVYFYNPSLKKMKITRVYVSQSFNINLVLMSAYEMLNVEQFAPLSRCRLVAYNSSMDTIIQSLESCTDPALTELRAAQNYSLDFLLEYRAEDQEFEVYPPNGITWYVFKVDLSTMAMDGPFLVYSAAREREASDVLRRSIALRLHISEQQFLLATVRATVPKAFVSYDPHPTPEALQHLQNMANTQFKSITYFYLNVPNTDAATLEMLGVPTVESVECASGGDVVDAAMMNGVAPGHMSSSNDYDWRRYKRDLVEPMSQPSPSHGHESNSEDSSLSDGDRTLVETDNMAHRGGGDSQVSSTSHSPQLSSPEDEAASHDAMMRVHAYCNGNGSYAAADVVDPLLLPTSTNHFFYATKVECVDVVGTGSSSGHQSDEEAQLRKPTRAYKLLVGTHMRMGAFKKHIEQLIQVPAAHFKLQRKHDNNLSNNQNNSLVHLIEGETLTVELGKTLEPDEFKAKIHFLRLADIDNETSKLPCVCEWVYNANTTAEQAKKELVAKLHRIDAKYATLSVQNCRIWLKGGRIPIKILSDDETLYCDMRSSIAAEFIVQECEEEVDPQPKDDSLTLFVRRWCPAKLEFGKFQEITLDQDSEIRLSLSQISDIPIDKLSYMKLNSNFPCTSISALSVNESSSWYSVPTTLDKYPLNSTQTGNIYLYKDRTVPARELTLEERRLMNAREKARLDRVGCVSTTRYAQRRERALKIYLDSPEKSSNVTASAPMDVHVNN

Target ORF information:

RefSeq Version	NM_206279.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 47, transcript variant D (Usp47), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206279.4

ORF Insert Sequence:

ATGACGGACA AGGAGAGCGA GCAGTGCACC GTCTCGGTCT TTGACCAGAC ACCCGGCTCG 
GAGCAAAAGA AGATCAACGT AGTGGTCCGC TCCCACTTCA CGGTGAAACG CGTGATTGAC 
CTTATTGGCA CGCAGTTCTC GTACGAAAAG TTCGAGCTGT TGCTGCAGCC ACACGATAAC 
AAGGATCTGG TCAATCTGAA TGCCCTGGAA TCTCAGCTGA TGTACGAAGT GGCTGGTTTT 
GAGCCCCAGC TGAAGAATCA CTTGATCCTG CTGCCCTCCG GCAGTTGGGA TGGTGACGTG 
ACCAAACGCT TTGAGTTACC CATTAAAAGA GTGGTAGTCA AAAAGGTTAT GAAGTCGGAC 
GGCGAGAAGG CCAAGAGTCC TGCCACCGGC GAGAAGAAGA AACGCGTAGT TGGTGAGAAA 
ACGAAAAAGA AGCCAGCTTC GGGATCTTCC TCTCCAAGCA AGGCCAAGAC GACGAGTGAG 
GACTCTTTGG CTAAGACCAG CATCAGTTCG GAATCAAGTC CCGAAAAGAC TTCCAAGATC 
AAGACGACTG CAGCAAAGAT CTCCAAGCCC GGTTCGGAAA AAGCTCCCAG GGCTAGCCCT 
GAAGAATGTC CCGAGCTGTC TACAGAGATA AACTCTAAAA ACACTTCTTC GGAGAGTCCT 
GTGGCCAAGA AAACGGCCAA GGTCACTTCT AAGCCGACTT TAGAGTTGCT GAGTCCAATC 
AAGCCGTCAT CACCCATCAA AGAGTTGGAC TGCGAACCAG TAGACACCCT GAGCAAGCAA 
CAGCTCTCTG AGCAACTGCA GCTGTATCCA CAAGATGCTG AACAATTGTC GGATGACGAC 
CTGGCACTCG GAGCATCTGC TAGTCCGACA ATGCTGGGAC CTGGATACGA TTACGGAGCT 
CCGACCGGTG ACTCGGACGT CGAGGGTGTG ACCGGAGTGA CAGACCCATC CACAATTGGA 
ACAGACGATG GTACGTACCC GGCTCTGTCA AACTTCTATC GTCGCAAGTA TGGAGGCGAT 
GAGTTGCGAG CATGGCAGAG AGTCAACACA ACGGGTGCGG ACTTCGTGTC CTCGGCCACC 
ACAGAAACTG AGGCAGAGGC AAGGCAGGCG AGCTTGGGTC CCAGGGGATA TGTTGGTTTG 
GTCAACCAAG CCATGACCTG CTATCTAAAC AGCTTGCTGC AGGCACTCTT TATGACACCC 
GAGTTCAGAA ATGCTCTGTA CCGCTGGGAG TTCGACAATG ACAACGAGGC CAAGAACATA 
CCATACCAAC TGCAAAAGCT CTTCCTCAAC TTGCAGACCT CGCCCAAAGC GGCGGTAGAA 
ACCACAGACC TGACTCGCAG CTTTGGGTGG GACTCGACGG AGGCATGGCA GCAGCACGAT 
ATCCAGGAAC TGTGCCGAGT TATGTTCGAC GCTCTGGAGC ACAAATTCAA GAACACTAAG 
CAGGCAAATC TCATCTCTAA TCTGTACGAG GGCAAGATGA ATGATTATGT CAAGTGTTTG 
GAGTGCAATA CCGAGAAGAC GCGCGAGGAC ACCTTTCTAG ATATCCCGCT CCCTGTGAGG 
CCCTTTGGAA GCAGCTCCGC ATACGGCAGC ATCGAGGAAG CTCTGCGTGC CTTCGTTCAG 
CCCGAAACAC TCGATGGCAA TAACCAGTAT CTGTGCGAGA AGTGCAAGAA AAAATGCGAC 
GCCCACAAGG GACTGCACTT TAAGTCCTTT CCCTACATCC TCACGCTGCA CCTTAAACGC 
TTTGACTTTG ACTACCAGAC CATGCACCGC ATCAAATTAA ACGACAGAGT GACCTTCCCT 
CAGACGCTCA ACCTGAACAC GTTCATTAAC CGAAGTGGAA ACAGCGGTGA GCAAAACTCT 
CAGCTCAACG GCACCGTGGA CGATTGCAGC ACGGCGGATA GTGGATCCGC CATGGAGGAC 
GATAATTTGA GCAGCGGCGT GGTGACCACA GCGAGCTCTA GTCAGCACGA AAACGATCTG 
AACGACGAGG ATGAAGGCAT CGACATGAGC AGCAGCACCA GCAAGAGCGC CAAGCAAGGA 
TCCGGTCCGT ATCTCTACGA ACTGTTCGCC ATCATGATTC ATTCAGGCAG CGCTTCGGGC 
GGACACTACT ATGCTTATAT TAAGGACTTC GACAACAACG AGTGGTTCTG TTTTAACGAT 
CAGAACGTGA CTAGTATCAC CCAAGAGGAC ATCCAACGTT CGTTTGGCGG ACCCAACGGG 
AGCTACTACT CCAGTGCCTA CACCTCCAGC ACCAATGCAT ACATGTTGAT GTACCGGCAG 
GTGGATGCCA AAAGAAACGA GCTAGTCGCC AAGGTGGCCG ACTTTCCTGA GCACATCAAA 
ACGCTGCTAC CAAAGCTGCA CTCGGAGGAG GAGACGCGCG TGTCCCGCCT GGGCAGGCAT 
ATCACGGTTA CGGACTTGGC TCTTCCGGAT TTGTACAAGC CGCGCGTCTA CTTCTATAAT 
CCCTCCCTGA AGAAGATGAA GATTACCAGA GTGTACGTAT CGCAGAGTTT CAACATCAAC 
TTGGTCCTCA TGTCGGCCTA TGAGATGTTG AATGTGGAAC AATTCGCGCC GCTTTCCCGC 
TGTCGTTTGG TGGCTTACAA CTCCTCGATG GACACGATTA TTCAGTCCCT GGAGAGTTGC 
ACAGACCCAG CTTTGACCGA ACTGCGCGCG GCGCAGAACT ACAGTCTCGA CTTCCTGCTG 
GAGTACCGGG CCGAGGACCA AGAGTTTGAG GTCTATCCGC CGAACGGCAT TACTTGGTAT 
GTGTTTAAGG TAGATCTATC GACCATGGCG ATGGACGGTC CCTTCCTGGT TTACTCAGCA 
GCGCGGGAGC GGGAAGCCAG CGACGTCCTT CGCCGCTCGA TCGCCCTCCG TTTGCACATT 
AGCGAGCAGC AGTTCCTACT CGCCACGGTG CGCGCCACGG TGCCGAAGGC TTTCGTGTCC 
TACGATCCGC ATCCGACGCC GGAGGCCCTG CAACATCTCC AGAACATGGC CAACACCCAG 
TTTAAGTCCA TAACATACTT CTACTTGAAC GTACCCAACA CGGATGCCGC GACCCTTGAG 
ATGCTCGGTG TGCCCACCGT TGAGTCAGTC GAGTGCGCCA GCGGGGGTGA TGTTGTGGAT 
GCTGCAATGA TGAATGGCGT GGCACCTGGT CACATGTCAT CCAGCAACGA CTACGATTGG 
AGGCGCTACA AGCGAGATCT AGTGGAACCC ATGTCACAAC CCAGTCCCAG CCACGGCCAC 
GAGTCCAACT CGGAGGACAG TAGTCTGAGC GACGGAGACC GAACTCTGGT GGAGACGGAC 
AACATGGCAC ATCGCGGTGG AGGCGACAGC CAAGTCAGTT CCACCAGTCA CTCGCCGCAG 
CTGTCTAGTC CGGAGGACGA AGCAGCCTCA CACGATGCCA TGATGCGCGT TCATGCCTAC 
TGCAATGGAA ACGGCAGTTA TGCGGCGGCC GATGTGGTGG ATCCGCTGCT CCTGCCCACC 
AGCACCAACC ACTTCTTCTA CGCCACAAAG GTCGAGTGCG TGGATGTGGT TGGCACCGGT 
TCCAGTTCTG GTCACCAGTC CGACGAAGAG GCTCAGCTGC GTAAGCCCAC GCGTGCCTAT 
AAGCTGCTGG TGGGCACACA CATGCGAATG GGGGCCTTTA AGAAACACAT CGAGCAGCTG 
ATCCAAGTGC CCGCCGCCCA CTTTAAACTG CAGCGGAAAC ACGATAATAA TCTGTCGAAC 
AACCAGAACA ACTCGTTGGT ACATCTCATA GAAGGCGAAA CACTGACGGT GGAGTTGGGT 
AAGACGCTGG AGCCGGACGA GTTCAAGGCC AAGATTCACT TCCTGCGACT GGCGGACATT 
GATAACGAGA CGTCCAAACT GCCTTGCGTA TGCGAGTGGG TCTACAATGC CAACACCACG 
GCGGAGCAGG CCAAGAAGGA ACTGGTGGCC AAGCTGCATC GCATAGACGC CAAGTACGCC 
ACACTGTCTG TGCAAAACTG TCGCATCTGG CTGAAGGGCG GACGTATTCC CATCAAAATT 
CTGTCCGACG ATGAAACGCT CTACTGCGAC ATGCGCTCCA GCATCGCAGC GGAGTTCATT 
GTGCAAGAGT GCGAGGAGGA AGTGGACCCG CAGCCCAAGG ACGATTCTCT GACTTTATTT 
GTAAGACGGT GGTGTCCGGC TAAACTGGAG TTTGGAAAGT TCCAAGAAAT CACTTTGGAC 
CAGGACAGCG AAATCAGACT TTCATTATCA CAGATCAGCG ACATCCCAAT AGACAAACTG 
AGCTATATGA AGTTGAACAG CAACTTCCCC TGCACAAGCA TATCAGCGTT AAGTGTTAAC 
GAGTCCAGCA GTTGGTACTC CGTGCCCACC ACCCTGGACA AATATCCGCT TAATAGCACA 
CAGACAGGAA ACATCTACCT GTACAAAGAT AGGACGGTAC CTGCGCGAGA GCTGACGCTG 
GAGGAGCGAC GCCTGATGAA CGCCAGGGAG AAGGCACGAC TGGATCGGGT GGGCTGCGTG 
TCCACCACGC GGTATGCGCA GCGCCGGGAA CGTGCCTTGA AGATTTACCT GGACTCTCCG 
GAGAAGTCGA GCAACGTGAC CGCCTCCGCA CCGATGGACG TGCATGTCAA TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp47 ( NM_168129.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729087.1 Drosophila melanogaster ubiquitin specific protease 47, transcript variant A (Usp47), mRNA.

Price & Availability↑

CloneID	OFa17618
Clone ID Related Accession (Same CDS sequence)	NM_168129.4 , NM_079213.4
Accession Version	NM_168129.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 47, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168129.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGACA AGGAGAGCGA GCAGTGCACC GTCTCGGTCT TTGACCAGAC ACCCGGCTCG 
GAGCAAAAGA AGATCAACGT AGTGGTCCGC TCCCACTTCA CGGTGAAACG CGTGATTGAC 
CTTATTGGCA CGCAGTTCTC GTACGAAAAG TTCGAGCTGT TGCTGCAGCC ACACGATAAC 
AAGGATCTGG TCAATCTGAA TGCCCTGGAA TCTCAGCTGA TGTACGAAGT GGCTGGTTTT 
GAGCCCCAGC TGAAGAATCA CTTGATCCTG CTGCCCTCCG GCAGTTGGGA TGGTGACGTG 
ACCAAACGCT TTGAGTTACC CATTAAAAGA GTGGTAGTCA AAAAGGTTAT GAAGTCGGAC 
GGCGAGAAGG CCAAGAGTCC TGCCACCGGC GAGAAGAAGA AACGCGTAGT TGGTGAGAAA 
ACGAAAAAGA AGCCAGCTTC GGGATCTTCC TCTCCAAGCA AGGCCAAGAC GACGAGTGAG 
GACTCTTTGG CTAAGACCAG CATCAGTTCG GAATCAAGTC CCGAAAAGAC TTCCAAGATC 
AAGACGACTG CAGCAAAGAT CTCCAAGCCC GGTTCGGAAA AAGCTCCCAG GGCTAGCCCT 
GAAGAATGTC CCGAGCTGTC TACAGAGATA AACTCTAAAA ACACTTCTTC GGAGAGTCCT 
GTGGCCAAGA AAACGGCCAA GGTCACTTCT AAGCCGACTT TAGAGTTGCT GAGTCCAATC 
AAGCCGTCAT CACCCATCAA AGAGTTGGAC TGCGAACCAG TAGACACCCT GAGCAAGCAA 
CAGCTCTCTG AGCAACTGCA GCTGTATCCA CAAGGTAGAA ACCTAATCTC ACCGGTGGAC 
GATGCTCCTT CAGATCTTTT CATCTCAGAT GCTGAACAAT TGTCGGATGA CGACCTGGCA 
CTCGGAGCAT CTGCTAGTCC GACAATGCTG GGACCTGGAT ACGATTACGG AGCTCCGACC 
GGTGACTCGG ACGTCGAGGG TGTGACCGGA GTGACAGACC CATCCACAAT TGGAACAGAC 
GATGGTACGT ACCCGGCTCT GTCAAACTTC TATCGTCGCA AGTATGGAGG CGATGAGTTG 
CGAGCATGGC AGAGAGTCAA CACAACGGGT GCGGACTTCG TGTCCTCGGC CACCACAGAA 
ACTGAGGCAG AGGCAAGGCA GGCGAGCTTG GGTCCCAGGG GATATGTTGG TTTGGTCAAC 
CAAGCCATGA CCTGCTATCT AAACAGCTTG CTGCAGGCAC TCTTTATGAC ACCCGAGTTC 
AGAAATGCTC TGTACCGCTG GGAGTTCGAC AATGACAACG AGGCCAAGAA CATACCATAC 
CAACTGCAAA AGCTCTTCCT CAACTTGCAG ACCTCGCCCA AAGCGGCGGT AGAAACCACA 
GACCTGACTC GCAGCTTTGG GTGGGACTCG ACGGAGGCAT GGCAGCAGCA CGATATCCAG 
GAACTGTGCC GAGTTATGTT CGACGCTCTG GAGCACAAAT TCAAGAACAC TAAGCAGGCA 
AATCTCATCT CTAATCTGTA CGAGGGCAAG ATGAATGATT ATGTCAAGTG TTTGGAGTGC 
AATACCGAGA AGACGCGCGA GGACACCTTT CTAGATATCC CGCTCCCTGT GAGGCCCTTT 
GGAAGCAGCT CCGCATACGG CAGCATCGAG GAAGCTCTGC GTGCCTTCGT TCAGCCCGAA 
ACACTCGATG GCAATAACCA GTATCTGTGC GAGAAGTGCA AGAAAAAATG CGACGCCCAC 
AAGGGACTGC ACTTTAAGTC CTTTCCCTAC ATCCTCACGC TGCACCTTAA ACGCTTTGAC 
TTTGACTACC AGACCATGCA CCGCATCAAA TTAAACGACA GAGTGACCTT CCCTCAGACG 
CTCAACCTGA ACACGTTCAT TAACCGAAGT GGAAACAGCG GTGAGCAAAA CTCTCAGCTC 
AACGGCACCG TGGACGATTG CAGCACGGCG GATAGTGGAT CCGCCATGGA GGACGATAAT 
TTGAGCAGCG GCGTGGTGAC CACAGCGAGC TCTAGTCAGC ACGAAAACGA TCTGAACGAC 
GAGGATGAAG GCATCGACAT GAGCAGCAGC ACCAGCAAGA GCGCCAAGCA AGGATCCGGT 
CCGTATCTCT ACGAACTGTT CGCCATCATG ATTCATTCAG GCAGCGCTTC GGGCGGACAC 
TACTATGCTT ATATTAAGGA CTTCGACAAC AACGAGTGGT TCTGTTTTAA CGATCAGAAC 
GTGACTAGTA TCACCCAAGA GGACATCCAA CGTTCGTTTG GCGGACCCAA CGGGAGCTAC 
TACTCCAGTG CCTACACCTC CAGCACCAAT GCATACATGT TGATGTACCG GCAGGTGGAT 
GCCAAAAGAA ACGAGCTAGT CGCCAAGGTG GCCGACTTTC CTGAGCACAT CAAAACGCTG 
CTACCAAAGC TGCACTCGGA GGAGGAGACG CGCGTGTCCC GCCTGGGCAG GCATATCACG 
GTTACGGACT TGGCTCTTCC GGATTTGTAC AAGCCGCGCG TCTACTTCTA TAATCCCTCC 
CTGAAGAAGA TGAAGATTAC CAGAGTGTAC GTATCGCAGA GTTTCAACAT CAACTTGGTC 
CTCATGTCGG CCTATGAGAT GTTGAATGTG GAACAATTCG CGCCGCTTTC CCGCTGTCGT 
TTGGTGGCTT ACAACTCCTC GATGGACACG ATTATTCAGT CCCTGGAGAG TTGCACAGAC 
CCAGCTTTGA CCGAACTGCG CGCGGCGCAG AACTACAGTC TCGACTTCCT GCTGGAGTAC 
CGGGCCGAGG ACCAAGAGTT TGAGGTCTAT CCGCCGAACG GCATTACTTG GTATGTGTTT 
AAGGTAGATC TATCGACCAT GGCGATGGAC GGTCCCTTCC TGGTTTACTC AGCAGCGCGG 
GAGCGGGAAG CCAGCGACGT CCTTCGCCGC TCGATCGCCC TCCGTTTGCA CATTAGCGAG 
CAGCAGTTCC TACTCGCCAC GGTGCGCGCC ACGGTGCCGA AGGCTTTCGT GTCCTACGAT 
CCGCATCCGA CGCCGGAGGC CCTGCAACAT CTCCAGAACA TGGCCAACAC CCAGTTTAAG 
TCCATAACAT ACTTCTACTT GAACGTACCC AACACGGATG CCGCGACCCT TGAGATGCTC 
GGTGTGCCCA CCGTTGAGTC AGTCGAGTGC GCCAGCGGGG GTGATGTTGT GGATGCTGCA 
ATGATGAATG GCGTGGCACC TGGTCACATG TCATCCAGCA ACGACTACGA TTGGAGGCGC 
TACAAGCGAG ATCTAGTGGA ACCCATGTCA CAACCCAGTC CCAGCCACGG CCACGAGTCC 
AACTCGGAGG ACAGTAGTCT GAGCGACGGA GACCGAACTC TGGTGGAGAC GGACAACATG 
GCACATCGCG GTGGAGGCGA CAGCCAAGTC AGTTCCACCA GTCACTCGCC GCAGCTGTCT 
AGTCCGGAGG ACGAAGCAGC CTCACACGAT GCCATGATGC GCGTTCATGC CTACTGCAAT 
GGAAACGGCA GTTATGCGGC GGCCGATGTG GTGGATCCGC TGCTCCTGCC CACCAGCACC 
AACCACTTCT TCTACGCCAC AAAGGTCGAG TGCGTGGATG TGGTTGGCAC CGGTTCCAGT 
TCTGGTCACC AGTCCGACGA AGAGGCTCAG CTGCGTAAGC CCACGCGTGC CTATAAGCTG 
CTGGTGGGCA CACACATGCG AATGGGGGCC TTTAAGAAAC ACATCGAGCA GCTGATCCAA 
GTGCCCGCCG CCCACTTTAA ACTGCAGCGG AAACACGATA ATAATCTGTC GAACAACCAG 
AACAACTCGT TGGTACATCT CATAGAAGGC GAAACACTGA CGGTGGAGTT GGGTAAGACG 
CTGGAGCCGG ACGAGTTCAA GGCCAAGATT CACTTCCTGC GACTGGCGGA CATTGATAAC 
GAGACGTCCA AACTGCCTTG CGTATGCGAG TGGGTCTACA ATGCCAACAC CACGGCGGAG 
CAGGCCAAGA AGGAACTGGT GGCCAAGCTG CATCGCATAG ACGCCAAGTA CGCCACACTG 
TCTGTGCAAA ACTGTCGCAT CTGGCTGAAG GGCGGACGTA TTCCCATCAA AATTCTGTCC 
GACGATGAAA CGCTCTACTG CGACATGCGC TCCAGCATCG CAGCGGAGTT CATTGTGCAA 
GAGTGCGAGG AGGAAGTGGA CCCGCAGCCC AAGGACGATT CTCTGACTTT ATTTGTAAGA 
CGGTGGTGTC CGGCTAAACT GGAGTTTGGA AAGTTCCAAG AAATCACTTT GGACCAGGAC 
AGCGAAATCA GACTTTCATT ATCACAGATC AGCGACATCC CAATAGACAA ACTGAGCTAT 
ATGAAGTTGA ACAGCAACTT CCCCTGCACA AGCATATCAG CGTTAAGTGT TAACGAGTCC 
AGCAGTTGGT ACTCCGTGCC CACCACCCTG GACAAATATC CGCTTAATAG CACACAGACA 
GGAAACATCT ACCTGTACAA AGATAGGACG GTACCTGCGC GAGAGCTGAC GCTGGAGGAG 
CGACGCCTGA TGAACGCCAG GGAGAAGGCA CGACTGGATC GGGTGGGCTG CGTGTCCACC 
ACGCGGTATG CGCAGCGCCG GGAACGTGCC TTGAAGATTT ACCTGGACTC TCCGGAGAAG 
TCGAGCAACG TGACCGCCTC CGCACCGATG GACGTGCATG TCAATAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729087.1
CDS	303..4973
Translation	MTDKESEQCTVSVFDQTPGSEQKKINVVVRSHFTVKRVIDLIGTQFSYEKFELLLQPHDNKDLVNLNALESQLMYEVAGFEPQLKNHLILLPSGSWDGDVTKRFELPIKRVVVKKVMKSDGEKAKSPATGEKKKRVVGEKTKKKPASGSSSPSKAKTTSEDSLAKTSISSESSPEKTSKIKTTAAKISKPGSEKAPRASPEECPELSTEINSKNTSSESPVAKKTAKVTSKPTLELLSPIKPSSPIKELDCEPVDTLSKQQLSEQLQLYPQGRNLISPVDDAPSDLFISDAEQLSDDDLALGASASPTMLGPGYDYGAPTGDSDVEGVTGVTDPSTIGTDDGTYPALSNFYRRKYGGDELRAWQRVNTTGADFVSSATTETEAEARQASLGPRGYVGLVNQAMTCYLNSLLQALFMTPEFRNALYRWEFDNDNEAKNIPYQLQKLFLNLQTSPKAAVETTDLTRSFGWDSTEAWQQHDIQELCRVMFDALEHKFKNTKQANLISNLYEGKMNDYVKCLECNTEKTREDTFLDIPLPVRPFGSSSAYGSIEEALRAFVQPETLDGNNQYLCEKCKKKCDAHKGLHFKSFPYILTLHLKRFDFDYQTMHRIKLNDRVTFPQTLNLNTFINRSGNSGEQNSQLNGTVDDCSTADSGSAMEDDNLSSGVVTTASSSQHENDLNDEDEGIDMSSSTSKSAKQGSGPYLYELFAIMIHSGSASGGHYYAYIKDFDNNEWFCFNDQNVTSITQEDIQRSFGGPNGSYYSSAYTSSTNAYMLMYRQVDAKRNELVAKVADFPEHIKTLLPKLHSEEETRVSRLGRHITVTDLALPDLYKPRVYFYNPSLKKMKITRVYVSQSFNINLVLMSAYEMLNVEQFAPLSRCRLVAYNSSMDTIIQSLESCTDPALTELRAAQNYSLDFLLEYRAEDQEFEVYPPNGITWYVFKVDLSTMAMDGPFLVYSAAREREASDVLRRSIALRLHISEQQFLLATVRATVPKAFVSYDPHPTPEALQHLQNMANTQFKSITYFYLNVPNTDAATLEMLGVPTVESVECASGGDVVDAAMMNGVAPGHMSSSNDYDWRRYKRDLVEPMSQPSPSHGHESNSEDSSLSDGDRTLVETDNMAHRGGGDSQVSSTSHSPQLSSPEDEAASHDAMMRVHAYCNGNGSYAAADVVDPLLLPTSTNHFFYATKVECVDVVGTGSSSGHQSDEEAQLRKPTRAYKLLVGTHMRMGAFKKHIEQLIQVPAAHFKLQRKHDNNLSNNQNNSLVHLIEGETLTVELGKTLEPDEFKAKIHFLRLADIDNETSKLPCVCEWVYNANTTAEQAKKELVAKLHRIDAKYATLSVQNCRIWLKGGRIPIKILSDDETLYCDMRSSIAAEFIVQECEEEVDPQPKDDSLTLFVRRWCPAKLEFGKFQEITLDQDSEIRLSLSQISDIPIDKLSYMKLNSNFPCTSISALSVNESSSWYSVPTTLDKYPLNSTQTGNIYLYKDRTVPARELTLEERRLMNAREKARLDRVGCVSTTRYAQRRERALKIYLDSPEKSSNVTASAPMDVHVNN

Target ORF information:

RefSeq Version	NM_168129.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 47, transcript variant A (Usp47), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168129.4

ORF Insert Sequence:

ATGACGGACA AGGAGAGCGA GCAGTGCACC GTCTCGGTCT TTGACCAGAC ACCCGGCTCG 
GAGCAAAAGA AGATCAACGT AGTGGTCCGC TCCCACTTCA CGGTGAAACG CGTGATTGAC 
CTTATTGGCA CGCAGTTCTC GTACGAAAAG TTCGAGCTGT TGCTGCAGCC ACACGATAAC 
AAGGATCTGG TCAATCTGAA TGCCCTGGAA TCTCAGCTGA TGTACGAAGT GGCTGGTTTT 
GAGCCCCAGC TGAAGAATCA CTTGATCCTG CTGCCCTCCG GCAGTTGGGA TGGTGACGTG 
ACCAAACGCT TTGAGTTACC CATTAAAAGA GTGGTAGTCA AAAAGGTTAT GAAGTCGGAC 
GGCGAGAAGG CCAAGAGTCC TGCCACCGGC GAGAAGAAGA AACGCGTAGT TGGTGAGAAA 
ACGAAAAAGA AGCCAGCTTC GGGATCTTCC TCTCCAAGCA AGGCCAAGAC GACGAGTGAG 
GACTCTTTGG CTAAGACCAG CATCAGTTCG GAATCAAGTC CCGAAAAGAC TTCCAAGATC 
AAGACGACTG CAGCAAAGAT CTCCAAGCCC GGTTCGGAAA AAGCTCCCAG GGCTAGCCCT 
GAAGAATGTC CCGAGCTGTC TACAGAGATA AACTCTAAAA ACACTTCTTC GGAGAGTCCT 
GTGGCCAAGA AAACGGCCAA GGTCACTTCT AAGCCGACTT TAGAGTTGCT GAGTCCAATC 
AAGCCGTCAT CACCCATCAA AGAGTTGGAC TGCGAACCAG TAGACACCCT GAGCAAGCAA 
CAGCTCTCTG AGCAACTGCA GCTGTATCCA CAAGGTAGAA ACCTAATCTC ACCGGTGGAC 
GATGCTCCTT CAGATCTTTT CATCTCAGAT GCTGAACAAT TGTCGGATGA CGACCTGGCA 
CTCGGAGCAT CTGCTAGTCC GACAATGCTG GGACCTGGAT ACGATTACGG AGCTCCGACC 
GGTGACTCGG ACGTCGAGGG TGTGACCGGA GTGACAGACC CATCCACAAT TGGAACAGAC 
GATGGTACGT ACCCGGCTCT GTCAAACTTC TATCGTCGCA AGTATGGAGG CGATGAGTTG 
CGAGCATGGC AGAGAGTCAA CACAACGGGT GCGGACTTCG TGTCCTCGGC CACCACAGAA 
ACTGAGGCAG AGGCAAGGCA GGCGAGCTTG GGTCCCAGGG GATATGTTGG TTTGGTCAAC 
CAAGCCATGA CCTGCTATCT AAACAGCTTG CTGCAGGCAC TCTTTATGAC ACCCGAGTTC 
AGAAATGCTC TGTACCGCTG GGAGTTCGAC AATGACAACG AGGCCAAGAA CATACCATAC 
CAACTGCAAA AGCTCTTCCT CAACTTGCAG ACCTCGCCCA AAGCGGCGGT AGAAACCACA 
GACCTGACTC GCAGCTTTGG GTGGGACTCG ACGGAGGCAT GGCAGCAGCA CGATATCCAG 
GAACTGTGCC GAGTTATGTT CGACGCTCTG GAGCACAAAT TCAAGAACAC TAAGCAGGCA 
AATCTCATCT CTAATCTGTA CGAGGGCAAG ATGAATGATT ATGTCAAGTG TTTGGAGTGC 
AATACCGAGA AGACGCGCGA GGACACCTTT CTAGATATCC CGCTCCCTGT GAGGCCCTTT 
GGAAGCAGCT CCGCATACGG CAGCATCGAG GAAGCTCTGC GTGCCTTCGT TCAGCCCGAA 
ACACTCGATG GCAATAACCA GTATCTGTGC GAGAAGTGCA AGAAAAAATG CGACGCCCAC 
AAGGGACTGC ACTTTAAGTC CTTTCCCTAC ATCCTCACGC TGCACCTTAA ACGCTTTGAC 
TTTGACTACC AGACCATGCA CCGCATCAAA TTAAACGACA GAGTGACCTT CCCTCAGACG 
CTCAACCTGA ACACGTTCAT TAACCGAAGT GGAAACAGCG GTGAGCAAAA CTCTCAGCTC 
AACGGCACCG TGGACGATTG CAGCACGGCG GATAGTGGAT CCGCCATGGA GGACGATAAT 
TTGAGCAGCG GCGTGGTGAC CACAGCGAGC TCTAGTCAGC ACGAAAACGA TCTGAACGAC 
GAGGATGAAG GCATCGACAT GAGCAGCAGC ACCAGCAAGA GCGCCAAGCA AGGATCCGGT 
CCGTATCTCT ACGAACTGTT CGCCATCATG ATTCATTCAG GCAGCGCTTC GGGCGGACAC 
TACTATGCTT ATATTAAGGA CTTCGACAAC AACGAGTGGT TCTGTTTTAA CGATCAGAAC 
GTGACTAGTA TCACCCAAGA GGACATCCAA CGTTCGTTTG GCGGACCCAA CGGGAGCTAC 
TACTCCAGTG CCTACACCTC CAGCACCAAT GCATACATGT TGATGTACCG GCAGGTGGAT 
GCCAAAAGAA ACGAGCTAGT CGCCAAGGTG GCCGACTTTC CTGAGCACAT CAAAACGCTG 
CTACCAAAGC TGCACTCGGA GGAGGAGACG CGCGTGTCCC GCCTGGGCAG GCATATCACG 
GTTACGGACT TGGCTCTTCC GGATTTGTAC AAGCCGCGCG TCTACTTCTA TAATCCCTCC 
CTGAAGAAGA TGAAGATTAC CAGAGTGTAC GTATCGCAGA GTTTCAACAT CAACTTGGTC 
CTCATGTCGG CCTATGAGAT GTTGAATGTG GAACAATTCG CGCCGCTTTC CCGCTGTCGT 
TTGGTGGCTT ACAACTCCTC GATGGACACG ATTATTCAGT CCCTGGAGAG TTGCACAGAC 
CCAGCTTTGA CCGAACTGCG CGCGGCGCAG AACTACAGTC TCGACTTCCT GCTGGAGTAC 
CGGGCCGAGG ACCAAGAGTT TGAGGTCTAT CCGCCGAACG GCATTACTTG GTATGTGTTT 
AAGGTAGATC TATCGACCAT GGCGATGGAC GGTCCCTTCC TGGTTTACTC AGCAGCGCGG 
GAGCGGGAAG CCAGCGACGT CCTTCGCCGC TCGATCGCCC TCCGTTTGCA CATTAGCGAG 
CAGCAGTTCC TACTCGCCAC GGTGCGCGCC ACGGTGCCGA AGGCTTTCGT GTCCTACGAT 
CCGCATCCGA CGCCGGAGGC CCTGCAACAT CTCCAGAACA TGGCCAACAC CCAGTTTAAG 
TCCATAACAT ACTTCTACTT GAACGTACCC AACACGGATG CCGCGACCCT TGAGATGCTC 
GGTGTGCCCA CCGTTGAGTC AGTCGAGTGC GCCAGCGGGG GTGATGTTGT GGATGCTGCA 
ATGATGAATG GCGTGGCACC TGGTCACATG TCATCCAGCA ACGACTACGA TTGGAGGCGC 
TACAAGCGAG ATCTAGTGGA ACCCATGTCA CAACCCAGTC CCAGCCACGG CCACGAGTCC 
AACTCGGAGG ACAGTAGTCT GAGCGACGGA GACCGAACTC TGGTGGAGAC GGACAACATG 
GCACATCGCG GTGGAGGCGA CAGCCAAGTC AGTTCCACCA GTCACTCGCC GCAGCTGTCT 
AGTCCGGAGG ACGAAGCAGC CTCACACGAT GCCATGATGC GCGTTCATGC CTACTGCAAT 
GGAAACGGCA GTTATGCGGC GGCCGATGTG GTGGATCCGC TGCTCCTGCC CACCAGCACC 
AACCACTTCT TCTACGCCAC AAAGGTCGAG TGCGTGGATG TGGTTGGCAC CGGTTCCAGT 
TCTGGTCACC AGTCCGACGA AGAGGCTCAG CTGCGTAAGC CCACGCGTGC CTATAAGCTG 
CTGGTGGGCA CACACATGCG AATGGGGGCC TTTAAGAAAC ACATCGAGCA GCTGATCCAA 
GTGCCCGCCG CCCACTTTAA ACTGCAGCGG AAACACGATA ATAATCTGTC GAACAACCAG 
AACAACTCGT TGGTACATCT CATAGAAGGC GAAACACTGA CGGTGGAGTT GGGTAAGACG 
CTGGAGCCGG ACGAGTTCAA GGCCAAGATT CACTTCCTGC GACTGGCGGA CATTGATAAC 
GAGACGTCCA AACTGCCTTG CGTATGCGAG TGGGTCTACA ATGCCAACAC CACGGCGGAG 
CAGGCCAAGA AGGAACTGGT GGCCAAGCTG CATCGCATAG ACGCCAAGTA CGCCACACTG 
TCTGTGCAAA ACTGTCGCAT CTGGCTGAAG GGCGGACGTA TTCCCATCAA AATTCTGTCC 
GACGATGAAA CGCTCTACTG CGACATGCGC TCCAGCATCG CAGCGGAGTT CATTGTGCAA 
GAGTGCGAGG AGGAAGTGGA CCCGCAGCCC AAGGACGATT CTCTGACTTT ATTTGTAAGA 
CGGTGGTGTC CGGCTAAACT GGAGTTTGGA AAGTTCCAAG AAATCACTTT GGACCAGGAC 
AGCGAAATCA GACTTTCATT ATCACAGATC AGCGACATCC CAATAGACAA ACTGAGCTAT 
ATGAAGTTGA ACAGCAACTT CCCCTGCACA AGCATATCAG CGTTAAGTGT TAACGAGTCC 
AGCAGTTGGT ACTCCGTGCC CACCACCCTG GACAAATATC CGCTTAATAG CACACAGACA 
GGAAACATCT ACCTGTACAA AGATAGGACG GTACCTGCGC GAGAGCTGAC GCTGGAGGAG 
CGACGCCTGA TGAACGCCAG GGAGAAGGCA CGACTGGATC GGGTGGGCTG CGTGTCCACC 
ACGCGGTATG CGCAGCGCCG GGAACGTGCC TTGAAGATTT ACCTGGACTC TCCGGAGAAG 
TCGAGCAACG TGACCGCCTC CGCACCGATG GACGTGCATG TCAATAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp47 ( NM_079213.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523937.2 Drosophila melanogaster ubiquitin specific protease 47, transcript variant B (Usp47), mRNA.

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CloneID	OFa17618
Clone ID Related Accession (Same CDS sequence)	NM_168129.4 , NM_079213.4
Accession Version	NM_079213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 47, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079213.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGACA AGGAGAGCGA GCAGTGCACC GTCTCGGTCT TTGACCAGAC ACCCGGCTCG 
GAGCAAAAGA AGATCAACGT AGTGGTCCGC TCCCACTTCA CGGTGAAACG CGTGATTGAC 
CTTATTGGCA CGCAGTTCTC GTACGAAAAG TTCGAGCTGT TGCTGCAGCC ACACGATAAC 
AAGGATCTGG TCAATCTGAA TGCCCTGGAA TCTCAGCTGA TGTACGAAGT GGCTGGTTTT 
GAGCCCCAGC TGAAGAATCA CTTGATCCTG CTGCCCTCCG GCAGTTGGGA TGGTGACGTG 
ACCAAACGCT TTGAGTTACC CATTAAAAGA GTGGTAGTCA AAAAGGTTAT GAAGTCGGAC 
GGCGAGAAGG CCAAGAGTCC TGCCACCGGC GAGAAGAAGA AACGCGTAGT TGGTGAGAAA 
ACGAAAAAGA AGCCAGCTTC GGGATCTTCC TCTCCAAGCA AGGCCAAGAC GACGAGTGAG 
GACTCTTTGG CTAAGACCAG CATCAGTTCG GAATCAAGTC CCGAAAAGAC TTCCAAGATC 
AAGACGACTG CAGCAAAGAT CTCCAAGCCC GGTTCGGAAA AAGCTCCCAG GGCTAGCCCT 
GAAGAATGTC CCGAGCTGTC TACAGAGATA AACTCTAAAA ACACTTCTTC GGAGAGTCCT 
GTGGCCAAGA AAACGGCCAA GGTCACTTCT AAGCCGACTT TAGAGTTGCT GAGTCCAATC 
AAGCCGTCAT CACCCATCAA AGAGTTGGAC TGCGAACCAG TAGACACCCT GAGCAAGCAA 
CAGCTCTCTG AGCAACTGCA GCTGTATCCA CAAGGTAGAA ACCTAATCTC ACCGGTGGAC 
GATGCTCCTT CAGATCTTTT CATCTCAGAT GCTGAACAAT TGTCGGATGA CGACCTGGCA 
CTCGGAGCAT CTGCTAGTCC GACAATGCTG GGACCTGGAT ACGATTACGG AGCTCCGACC 
GGTGACTCGG ACGTCGAGGG TGTGACCGGA GTGACAGACC CATCCACAAT TGGAACAGAC 
GATGGTACGT ACCCGGCTCT GTCAAACTTC TATCGTCGCA AGTATGGAGG CGATGAGTTG 
CGAGCATGGC AGAGAGTCAA CACAACGGGT GCGGACTTCG TGTCCTCGGC CACCACAGAA 
ACTGAGGCAG AGGCAAGGCA GGCGAGCTTG GGTCCCAGGG GATATGTTGG TTTGGTCAAC 
CAAGCCATGA CCTGCTATCT AAACAGCTTG CTGCAGGCAC TCTTTATGAC ACCCGAGTTC 
AGAAATGCTC TGTACCGCTG GGAGTTCGAC AATGACAACG AGGCCAAGAA CATACCATAC 
CAACTGCAAA AGCTCTTCCT CAACTTGCAG ACCTCGCCCA AAGCGGCGGT AGAAACCACA 
GACCTGACTC GCAGCTTTGG GTGGGACTCG ACGGAGGCAT GGCAGCAGCA CGATATCCAG 
GAACTGTGCC GAGTTATGTT CGACGCTCTG GAGCACAAAT TCAAGAACAC TAAGCAGGCA 
AATCTCATCT CTAATCTGTA CGAGGGCAAG ATGAATGATT ATGTCAAGTG TTTGGAGTGC 
AATACCGAGA AGACGCGCGA GGACACCTTT CTAGATATCC CGCTCCCTGT GAGGCCCTTT 
GGAAGCAGCT CCGCATACGG CAGCATCGAG GAAGCTCTGC GTGCCTTCGT TCAGCCCGAA 
ACACTCGATG GCAATAACCA GTATCTGTGC GAGAAGTGCA AGAAAAAATG CGACGCCCAC 
AAGGGACTGC ACTTTAAGTC CTTTCCCTAC ATCCTCACGC TGCACCTTAA ACGCTTTGAC 
TTTGACTACC AGACCATGCA CCGCATCAAA TTAAACGACA GAGTGACCTT CCCTCAGACG 
CTCAACCTGA ACACGTTCAT TAACCGAAGT GGAAACAGCG GTGAGCAAAA CTCTCAGCTC 
AACGGCACCG TGGACGATTG CAGCACGGCG GATAGTGGAT CCGCCATGGA GGACGATAAT 
TTGAGCAGCG GCGTGGTGAC CACAGCGAGC TCTAGTCAGC ACGAAAACGA TCTGAACGAC 
GAGGATGAAG GCATCGACAT GAGCAGCAGC ACCAGCAAGA GCGCCAAGCA AGGATCCGGT 
CCGTATCTCT ACGAACTGTT CGCCATCATG ATTCATTCAG GCAGCGCTTC GGGCGGACAC 
TACTATGCTT ATATTAAGGA CTTCGACAAC AACGAGTGGT TCTGTTTTAA CGATCAGAAC 
GTGACTAGTA TCACCCAAGA GGACATCCAA CGTTCGTTTG GCGGACCCAA CGGGAGCTAC 
TACTCCAGTG CCTACACCTC CAGCACCAAT GCATACATGT TGATGTACCG GCAGGTGGAT 
GCCAAAAGAA ACGAGCTAGT CGCCAAGGTG GCCGACTTTC CTGAGCACAT CAAAACGCTG 
CTACCAAAGC TGCACTCGGA GGAGGAGACG CGCGTGTCCC GCCTGGGCAG GCATATCACG 
GTTACGGACT TGGCTCTTCC GGATTTGTAC AAGCCGCGCG TCTACTTCTA TAATCCCTCC 
CTGAAGAAGA TGAAGATTAC CAGAGTGTAC GTATCGCAGA GTTTCAACAT CAACTTGGTC 
CTCATGTCGG CCTATGAGAT GTTGAATGTG GAACAATTCG CGCCGCTTTC CCGCTGTCGT 
TTGGTGGCTT ACAACTCCTC GATGGACACG ATTATTCAGT CCCTGGAGAG TTGCACAGAC 
CCAGCTTTGA CCGAACTGCG CGCGGCGCAG AACTACAGTC TCGACTTCCT GCTGGAGTAC 
CGGGCCGAGG ACCAAGAGTT TGAGGTCTAT CCGCCGAACG GCATTACTTG GTATGTGTTT 
AAGGTAGATC TATCGACCAT GGCGATGGAC GGTCCCTTCC TGGTTTACTC AGCAGCGCGG 
GAGCGGGAAG CCAGCGACGT CCTTCGCCGC TCGATCGCCC TCCGTTTGCA CATTAGCGAG 
CAGCAGTTCC TACTCGCCAC GGTGCGCGCC ACGGTGCCGA AGGCTTTCGT GTCCTACGAT 
CCGCATCCGA CGCCGGAGGC CCTGCAACAT CTCCAGAACA TGGCCAACAC CCAGTTTAAG 
TCCATAACAT ACTTCTACTT GAACGTACCC AACACGGATG CCGCGACCCT TGAGATGCTC 
GGTGTGCCCA CCGTTGAGTC AGTCGAGTGC GCCAGCGGGG GTGATGTTGT GGATGCTGCA 
ATGATGAATG GCGTGGCACC TGGTCACATG TCATCCAGCA ACGACTACGA TTGGAGGCGC 
TACAAGCGAG ATCTAGTGGA ACCCATGTCA CAACCCAGTC CCAGCCACGG CCACGAGTCC 
AACTCGGAGG ACAGTAGTCT GAGCGACGGA GACCGAACTC TGGTGGAGAC GGACAACATG 
GCACATCGCG GTGGAGGCGA CAGCCAAGTC AGTTCCACCA GTCACTCGCC GCAGCTGTCT 
AGTCCGGAGG ACGAAGCAGC CTCACACGAT GCCATGATGC GCGTTCATGC CTACTGCAAT 
GGAAACGGCA GTTATGCGGC GGCCGATGTG GTGGATCCGC TGCTCCTGCC CACCAGCACC 
AACCACTTCT TCTACGCCAC AAAGGTCGAG TGCGTGGATG TGGTTGGCAC CGGTTCCAGT 
TCTGGTCACC AGTCCGACGA AGAGGCTCAG CTGCGTAAGC CCACGCGTGC CTATAAGCTG 
CTGGTGGGCA CACACATGCG AATGGGGGCC TTTAAGAAAC ACATCGAGCA GCTGATCCAA 
GTGCCCGCCG CCCACTTTAA ACTGCAGCGG AAACACGATA ATAATCTGTC GAACAACCAG 
AACAACTCGT TGGTACATCT CATAGAAGGC GAAACACTGA CGGTGGAGTT GGGTAAGACG 
CTGGAGCCGG ACGAGTTCAA GGCCAAGATT CACTTCCTGC GACTGGCGGA CATTGATAAC 
GAGACGTCCA AACTGCCTTG CGTATGCGAG TGGGTCTACA ATGCCAACAC CACGGCGGAG 
CAGGCCAAGA AGGAACTGGT GGCCAAGCTG CATCGCATAG ACGCCAAGTA CGCCACACTG 
TCTGTGCAAA ACTGTCGCAT CTGGCTGAAG GGCGGACGTA TTCCCATCAA AATTCTGTCC 
GACGATGAAA CGCTCTACTG CGACATGCGC TCCAGCATCG CAGCGGAGTT CATTGTGCAA 
GAGTGCGAGG AGGAAGTGGA CCCGCAGCCC AAGGACGATT CTCTGACTTT ATTTGTAAGA 
CGGTGGTGTC CGGCTAAACT GGAGTTTGGA AAGTTCCAAG AAATCACTTT GGACCAGGAC 
AGCGAAATCA GACTTTCATT ATCACAGATC AGCGACATCC CAATAGACAA ACTGAGCTAT 
ATGAAGTTGA ACAGCAACTT CCCCTGCACA AGCATATCAG CGTTAAGTGT TAACGAGTCC 
AGCAGTTGGT ACTCCGTGCC CACCACCCTG GACAAATATC CGCTTAATAG CACACAGACA 
GGAAACATCT ACCTGTACAA AGATAGGACG GTACCTGCGC GAGAGCTGAC GCTGGAGGAG 
CGACGCCTGA TGAACGCCAG GGAGAAGGCA CGACTGGATC GGGTGGGCTG CGTGTCCACC 
ACGCGGTATG CGCAGCGCCG GGAACGTGCC TTGAAGATTT ACCTGGACTC TCCGGAGAAG 
TCGAGCAACG TGACCGCCTC CGCACCGATG GACGTGCATG TCAATAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523937.2
CDS	130..4800
Translation	MTDKESEQCTVSVFDQTPGSEQKKINVVVRSHFTVKRVIDLIGTQFSYEKFELLLQPHDNKDLVNLNALESQLMYEVAGFEPQLKNHLILLPSGSWDGDVTKRFELPIKRVVVKKVMKSDGEKAKSPATGEKKKRVVGEKTKKKPASGSSSPSKAKTTSEDSLAKTSISSESSPEKTSKIKTTAAKISKPGSEKAPRASPEECPELSTEINSKNTSSESPVAKKTAKVTSKPTLELLSPIKPSSPIKELDCEPVDTLSKQQLSEQLQLYPQGRNLISPVDDAPSDLFISDAEQLSDDDLALGASASPTMLGPGYDYGAPTGDSDVEGVTGVTDPSTIGTDDGTYPALSNFYRRKYGGDELRAWQRVNTTGADFVSSATTETEAEARQASLGPRGYVGLVNQAMTCYLNSLLQALFMTPEFRNALYRWEFDNDNEAKNIPYQLQKLFLNLQTSPKAAVETTDLTRSFGWDSTEAWQQHDIQELCRVMFDALEHKFKNTKQANLISNLYEGKMNDYVKCLECNTEKTREDTFLDIPLPVRPFGSSSAYGSIEEALRAFVQPETLDGNNQYLCEKCKKKCDAHKGLHFKSFPYILTLHLKRFDFDYQTMHRIKLNDRVTFPQTLNLNTFINRSGNSGEQNSQLNGTVDDCSTADSGSAMEDDNLSSGVVTTASSSQHENDLNDEDEGIDMSSSTSKSAKQGSGPYLYELFAIMIHSGSASGGHYYAYIKDFDNNEWFCFNDQNVTSITQEDIQRSFGGPNGSYYSSAYTSSTNAYMLMYRQVDAKRNELVAKVADFPEHIKTLLPKLHSEEETRVSRLGRHITVTDLALPDLYKPRVYFYNPSLKKMKITRVYVSQSFNINLVLMSAYEMLNVEQFAPLSRCRLVAYNSSMDTIIQSLESCTDPALTELRAAQNYSLDFLLEYRAEDQEFEVYPPNGITWYVFKVDLSTMAMDGPFLVYSAAREREASDVLRRSIALRLHISEQQFLLATVRATVPKAFVSYDPHPTPEALQHLQNMANTQFKSITYFYLNVPNTDAATLEMLGVPTVESVECASGGDVVDAAMMNGVAPGHMSSSNDYDWRRYKRDLVEPMSQPSPSHGHESNSEDSSLSDGDRTLVETDNMAHRGGGDSQVSSTSHSPQLSSPEDEAASHDAMMRVHAYCNGNGSYAAADVVDPLLLPTSTNHFFYATKVECVDVVGTGSSSGHQSDEEAQLRKPTRAYKLLVGTHMRMGAFKKHIEQLIQVPAAHFKLQRKHDNNLSNNQNNSLVHLIEGETLTVELGKTLEPDEFKAKIHFLRLADIDNETSKLPCVCEWVYNANTTAEQAKKELVAKLHRIDAKYATLSVQNCRIWLKGGRIPIKILSDDETLYCDMRSSIAAEFIVQECEEEVDPQPKDDSLTLFVRRWCPAKLEFGKFQEITLDQDSEIRLSLSQISDIPIDKLSYMKLNSNFPCTSISALSVNESSSWYSVPTTLDKYPLNSTQTGNIYLYKDRTVPARELTLEERRLMNAREKARLDRVGCVSTTRYAQRRERALKIYLDSPEKSSNVTASAPMDVHVNN

Target ORF information:

RefSeq Version	NM_079213.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 47, transcript variant B (Usp47), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079213.4

ORF Insert Sequence:

ATGACGGACA AGGAGAGCGA GCAGTGCACC GTCTCGGTCT TTGACCAGAC ACCCGGCTCG 
GAGCAAAAGA AGATCAACGT AGTGGTCCGC TCCCACTTCA CGGTGAAACG CGTGATTGAC 
CTTATTGGCA CGCAGTTCTC GTACGAAAAG TTCGAGCTGT TGCTGCAGCC ACACGATAAC 
AAGGATCTGG TCAATCTGAA TGCCCTGGAA TCTCAGCTGA TGTACGAAGT GGCTGGTTTT 
GAGCCCCAGC TGAAGAATCA CTTGATCCTG CTGCCCTCCG GCAGTTGGGA TGGTGACGTG 
ACCAAACGCT TTGAGTTACC CATTAAAAGA GTGGTAGTCA AAAAGGTTAT GAAGTCGGAC 
GGCGAGAAGG CCAAGAGTCC TGCCACCGGC GAGAAGAAGA AACGCGTAGT TGGTGAGAAA 
ACGAAAAAGA AGCCAGCTTC GGGATCTTCC TCTCCAAGCA AGGCCAAGAC GACGAGTGAG 
GACTCTTTGG CTAAGACCAG CATCAGTTCG GAATCAAGTC CCGAAAAGAC TTCCAAGATC 
AAGACGACTG CAGCAAAGAT CTCCAAGCCC GGTTCGGAAA AAGCTCCCAG GGCTAGCCCT 
GAAGAATGTC CCGAGCTGTC TACAGAGATA AACTCTAAAA ACACTTCTTC GGAGAGTCCT 
GTGGCCAAGA AAACGGCCAA GGTCACTTCT AAGCCGACTT TAGAGTTGCT GAGTCCAATC 
AAGCCGTCAT CACCCATCAA AGAGTTGGAC TGCGAACCAG TAGACACCCT GAGCAAGCAA 
CAGCTCTCTG AGCAACTGCA GCTGTATCCA CAAGGTAGAA ACCTAATCTC ACCGGTGGAC 
GATGCTCCTT CAGATCTTTT CATCTCAGAT GCTGAACAAT TGTCGGATGA CGACCTGGCA 
CTCGGAGCAT CTGCTAGTCC GACAATGCTG GGACCTGGAT ACGATTACGG AGCTCCGACC 
GGTGACTCGG ACGTCGAGGG TGTGACCGGA GTGACAGACC CATCCACAAT TGGAACAGAC 
GATGGTACGT ACCCGGCTCT GTCAAACTTC TATCGTCGCA AGTATGGAGG CGATGAGTTG 
CGAGCATGGC AGAGAGTCAA CACAACGGGT GCGGACTTCG TGTCCTCGGC CACCACAGAA 
ACTGAGGCAG AGGCAAGGCA GGCGAGCTTG GGTCCCAGGG GATATGTTGG TTTGGTCAAC 
CAAGCCATGA CCTGCTATCT AAACAGCTTG CTGCAGGCAC TCTTTATGAC ACCCGAGTTC 
AGAAATGCTC TGTACCGCTG GGAGTTCGAC AATGACAACG AGGCCAAGAA CATACCATAC 
CAACTGCAAA AGCTCTTCCT CAACTTGCAG ACCTCGCCCA AAGCGGCGGT AGAAACCACA 
GACCTGACTC GCAGCTTTGG GTGGGACTCG ACGGAGGCAT GGCAGCAGCA CGATATCCAG 
GAACTGTGCC GAGTTATGTT CGACGCTCTG GAGCACAAAT TCAAGAACAC TAAGCAGGCA 
AATCTCATCT CTAATCTGTA CGAGGGCAAG ATGAATGATT ATGTCAAGTG TTTGGAGTGC 
AATACCGAGA AGACGCGCGA GGACACCTTT CTAGATATCC CGCTCCCTGT GAGGCCCTTT 
GGAAGCAGCT CCGCATACGG CAGCATCGAG GAAGCTCTGC GTGCCTTCGT TCAGCCCGAA 
ACACTCGATG GCAATAACCA GTATCTGTGC GAGAAGTGCA AGAAAAAATG CGACGCCCAC 
AAGGGACTGC ACTTTAAGTC CTTTCCCTAC ATCCTCACGC TGCACCTTAA ACGCTTTGAC 
TTTGACTACC AGACCATGCA CCGCATCAAA TTAAACGACA GAGTGACCTT CCCTCAGACG 
CTCAACCTGA ACACGTTCAT TAACCGAAGT GGAAACAGCG GTGAGCAAAA CTCTCAGCTC 
AACGGCACCG TGGACGATTG CAGCACGGCG GATAGTGGAT CCGCCATGGA GGACGATAAT 
TTGAGCAGCG GCGTGGTGAC CACAGCGAGC TCTAGTCAGC ACGAAAACGA TCTGAACGAC 
GAGGATGAAG GCATCGACAT GAGCAGCAGC ACCAGCAAGA GCGCCAAGCA AGGATCCGGT 
CCGTATCTCT ACGAACTGTT CGCCATCATG ATTCATTCAG GCAGCGCTTC GGGCGGACAC 
TACTATGCTT ATATTAAGGA CTTCGACAAC AACGAGTGGT TCTGTTTTAA CGATCAGAAC 
GTGACTAGTA TCACCCAAGA GGACATCCAA CGTTCGTTTG GCGGACCCAA CGGGAGCTAC 
TACTCCAGTG CCTACACCTC CAGCACCAAT GCATACATGT TGATGTACCG GCAGGTGGAT 
GCCAAAAGAA ACGAGCTAGT CGCCAAGGTG GCCGACTTTC CTGAGCACAT CAAAACGCTG 
CTACCAAAGC TGCACTCGGA GGAGGAGACG CGCGTGTCCC GCCTGGGCAG GCATATCACG 
GTTACGGACT TGGCTCTTCC GGATTTGTAC AAGCCGCGCG TCTACTTCTA TAATCCCTCC 
CTGAAGAAGA TGAAGATTAC CAGAGTGTAC GTATCGCAGA GTTTCAACAT CAACTTGGTC 
CTCATGTCGG CCTATGAGAT GTTGAATGTG GAACAATTCG CGCCGCTTTC CCGCTGTCGT 
TTGGTGGCTT ACAACTCCTC GATGGACACG ATTATTCAGT CCCTGGAGAG TTGCACAGAC 
CCAGCTTTGA CCGAACTGCG CGCGGCGCAG AACTACAGTC TCGACTTCCT GCTGGAGTAC 
CGGGCCGAGG ACCAAGAGTT TGAGGTCTAT CCGCCGAACG GCATTACTTG GTATGTGTTT 
AAGGTAGATC TATCGACCAT GGCGATGGAC GGTCCCTTCC TGGTTTACTC AGCAGCGCGG 
GAGCGGGAAG CCAGCGACGT CCTTCGCCGC TCGATCGCCC TCCGTTTGCA CATTAGCGAG 
CAGCAGTTCC TACTCGCCAC GGTGCGCGCC ACGGTGCCGA AGGCTTTCGT GTCCTACGAT 
CCGCATCCGA CGCCGGAGGC CCTGCAACAT CTCCAGAACA TGGCCAACAC CCAGTTTAAG 
TCCATAACAT ACTTCTACTT GAACGTACCC AACACGGATG CCGCGACCCT TGAGATGCTC 
GGTGTGCCCA CCGTTGAGTC AGTCGAGTGC GCCAGCGGGG GTGATGTTGT GGATGCTGCA 
ATGATGAATG GCGTGGCACC TGGTCACATG TCATCCAGCA ACGACTACGA TTGGAGGCGC 
TACAAGCGAG ATCTAGTGGA ACCCATGTCA CAACCCAGTC CCAGCCACGG CCACGAGTCC 
AACTCGGAGG ACAGTAGTCT GAGCGACGGA GACCGAACTC TGGTGGAGAC GGACAACATG 
GCACATCGCG GTGGAGGCGA CAGCCAAGTC AGTTCCACCA GTCACTCGCC GCAGCTGTCT 
AGTCCGGAGG ACGAAGCAGC CTCACACGAT GCCATGATGC GCGTTCATGC CTACTGCAAT 
GGAAACGGCA GTTATGCGGC GGCCGATGTG GTGGATCCGC TGCTCCTGCC CACCAGCACC 
AACCACTTCT TCTACGCCAC AAAGGTCGAG TGCGTGGATG TGGTTGGCAC CGGTTCCAGT 
TCTGGTCACC AGTCCGACGA AGAGGCTCAG CTGCGTAAGC CCACGCGTGC CTATAAGCTG 
CTGGTGGGCA CACACATGCG AATGGGGGCC TTTAAGAAAC ACATCGAGCA GCTGATCCAA 
GTGCCCGCCG CCCACTTTAA ACTGCAGCGG AAACACGATA ATAATCTGTC GAACAACCAG 
AACAACTCGT TGGTACATCT CATAGAAGGC GAAACACTGA CGGTGGAGTT GGGTAAGACG 
CTGGAGCCGG ACGAGTTCAA GGCCAAGATT CACTTCCTGC GACTGGCGGA CATTGATAAC 
GAGACGTCCA AACTGCCTTG CGTATGCGAG TGGGTCTACA ATGCCAACAC CACGGCGGAG 
CAGGCCAAGA AGGAACTGGT GGCCAAGCTG CATCGCATAG ACGCCAAGTA CGCCACACTG 
TCTGTGCAAA ACTGTCGCAT CTGGCTGAAG GGCGGACGTA TTCCCATCAA AATTCTGTCC 
GACGATGAAA CGCTCTACTG CGACATGCGC TCCAGCATCG CAGCGGAGTT CATTGTGCAA 
GAGTGCGAGG AGGAAGTGGA CCCGCAGCCC AAGGACGATT CTCTGACTTT ATTTGTAAGA 
CGGTGGTGTC CGGCTAAACT GGAGTTTGGA AAGTTCCAAG AAATCACTTT GGACCAGGAC 
AGCGAAATCA GACTTTCATT ATCACAGATC AGCGACATCC CAATAGACAA ACTGAGCTAT 
ATGAAGTTGA ACAGCAACTT CCCCTGCACA AGCATATCAG CGTTAAGTGT TAACGAGTCC 
AGCAGTTGGT ACTCCGTGCC CACCACCCTG GACAAATATC CGCTTAATAG CACACAGACA 
GGAAACATCT ACCTGTACAA AGATAGGACG GTACCTGCGC GAGAGCTGAC GCTGGAGGAG 
CGACGCCTGA TGAACGCCAG GGAGAAGGCA CGACTGGATC GGGTGGGCTG CGTGTCCACC 
ACGCGGTATG CGCAGCGCCG GGAACGTGCC TTGAAGATTT ACCTGGACTC TCCGGAGAAG 
TCGAGCAACG TGACCGCCTC CGCACCGATG GACGTGCATG TCAATAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Usp47 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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