USP47 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP47
Entrez Gene ID	74996
Full Name	ubiquitin specific peptidase 47
Synonyms	4930502N04Rik,A630020C16Rik
General protein information	Preferred Names ubiquitin specific peptidase 47 Names ubiquitin carboxyl-terminal hydrolase 47 deubiquitinating enzyme 47 ubiquitin specific protease 47 ubiquitin thioesterase 47 ubiquitin thiolesterase 47 ubiquitin-specific-processing protease 47
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006508273.3	XP_006508336.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
XM_006508273.4	XP_006508336.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
NM_001357952.1	NP_001344881.1	ubiquitin carboxyl-terminal hydrolase 47 isoform 3
NM_133758.3	NP_598519.2	ubiquitin carboxyl-terminal hydrolase 47 isoform 2
NM_177249.3	NP_796223.2	ubiquitin carboxyl-terminal hydrolase 47 isoform 1
XM_030243044.1	XP_030098904.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
XM_006508274.3	XP_006508337.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP47	NP_001269588.1
Mus musculus (house mouse)	Usp47	NP_796223.2
Pan troglodytes (chimpanzee)	USP47	XP_001171097.1
Rattus norvegicus (Norway rat)	Usp47	NP_001101012.2
Gallus gallus (chicken)	USP47	NP_001186511.1
Drosophila melanogaster (fruit fly)	Ubp64E	NP_996001.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007106	XP_001237119.2
Xenopus tropicalis (tropical clawed frog)	usp47	NP_001090710.1
Canis lupus familiaris (dog)	USP47	XP_005633729.1
Bos taurus (cattle)	USP47	NP_001230219.1
	USP47	XP_001098399.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The ubiquitin-specific protease USP47 is a novel beta-TRCP interactor regulating cell survival.
Peschiaroli A, Skaar JR, Pagano M, Melino G
Oncogene29(9)1384-93(2010 Mar)

Phosphoproteomic analysis of the developing mouse brain.
Ballif BA, Vill?n J, Beausoleil SA, Schwartz D, Gygi SP
Molecular & cellular proteomics : MCP3(11)1093-101(2004 Nov)

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Kitamura H, Nakagawa T, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following USP47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu39919	XM_006508273.4 Latest version!	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu89642	XM_030243044.1 Latest version!	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu06121	NM_177249.3 Latest version!	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu10186	NM_133758.3 Latest version!	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu39920	NM_001357952.1 Latest version!	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu39920	XM_006508274.3 Latest version!	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu39919	XM_006508273.3	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP47 ( XM_006508273.4 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006508336.1 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39919
Clone ID Related Accession (Same CDS sequence)	XM_006508273.3 , XM_006508273.4
Accession Version	XM_006508273.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508273.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAAAATATCT TTGATGAAAT GAAGAAGAAA TTTTTACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508336.1
CDS	233..4423
Translation	MVPGEENQLVPKEDVFWRCRQNIFDEMKKKFLQIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKVEMAETDQRKDPIQFQLGEPNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	XM_006508273.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508273.4

ORF Insert Sequence:

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAAAATATCT TTGATGAAAT GAAGAAGAAA TTTTTACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_030243044.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030098904.1 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu89642
Clone ID Related Accession (Same CDS sequence)	XM_030243044.1
Accession Version	XM_030243044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGA AATTTTTACA GATAGAAAAT GCTGCTGAAG AACCTAGAGT CTTATGTATA 
ATACAAGACA CTACTAACTC GAAGACAGTG AGTGAACGGA TCACATTAAA TTTACCAGCA 
TCTACCCCAG TCCGAAAGCT CTTTGAAGAT GTGGCCAACA AAGTAGGCTA CATAAATGGA 
ACCTTTGATT TGACCAGGGA AAATGGAGTC ACTACTGCTG ACATGGCACC ACTGGATCAC 
ACCAGTGACA AGTCTCTTCT TGATGCTAAT TTTGAGCCAG GAAAGAAGAA CTTTCTGCAT 
TTGACAGATA AAGATGGTGA ACCGCCTCAG ATGTTGTTGG AGGACTCCAA TAACGTGGAT 
GACAGCGTGC ATGACAGGTT CATAGGTCCA CTGCCAAGAG AAGGTTCTGT GGCCTCTACC 
AATGATTATG TCAGCCAGAA CTACTCCTAT TCATCAATTT TGAACAAGTC AGAAACTGGT 
TATGTGGGAT TAGTAAACCA GGCGATGACG TGCTATTTGA ATAGCCTTTT GCAAACACTT 
TTTATGACTC CTGAATTTAG GAATGCATTA TATAAGTGGG AATTTGAAGA CTCTGAAGAA 
GATCCTGTAA CGAGTATCCC ATACCAGCTT CAAAGGCTTT TTGTTTTGTT ACAAACCAGC 
AAAAAGAGAG CAATTGAGAC CACAGATGTT ACAAGGAGCT TTGGATGGGA TAGCAGTGAG 
GCTTGGCAAC AGCATGATGT ACAAGAACTG TGCCGTGTTA TGTTTGATGC TTTGGAACAG 
AAATGGAAGC AAACAGAACA GGCTGATCTT ATAAATGAGC TGTACCAAGG CAAGCTGAAA 
GATTATGTGA GGTGTCTGGA ATGCGGTTAT GAAGGCTGGA GAATTGACAC GTATCTTGAT 
ATTCCACTGG TCATCCGACC TTATGGGTCC AGCCAAGCGT TTGCTAGTGT GGAAGAAGCG 
TTGCATGCAT TTATTCAACC AGAGATCTTG GATGGCCCAA ATCAATATTT TTGTGAACGT 
TGTAAGAAGA AGTGTGATGC TCGGAAGGGC CTTCGGTTTT TGCATTTCCC CTATCTGCTG 
ACCTTACAGT TGAAAAGGTT TGATTTTGAT TATACAACCA TGCATAGAAT TAAACTGAAT 
GATCGAATGT CATTTCCGGA GGAGCTGGAT ATGAGCACAT TTATTGATAT TGAAGATGAG 
AAATCTCCTC AGACTGAAAG TTGCACTGAT AGTGGAGCAG AAAATGAAGG CAGTTGCCAC 
AGCGACCAGA TGAGTAATGA TTTCTCCACG GATGATGCTG TTGATGAAGG GATATGCCTG 
GAGAGCAGTA GTGGGAGTGA GAAGATTTCA AAACCTGGCC TGGAAAAGGT AGAGATGGCG 
GAGACAGACC AGAGAAAGGA TCCCATCCAA TTCCAGCTTG GTGAACCAAA CTCCCTGATG 
TATGAGCTCT TCTCAGTGAT GGTGCATTCA GGGAGTGCTG CTGGAGGACA CTATTATGCT 
TGCATAAAGT CTTTCAGTGA CGATCAATGG TACAGTTTCA ATGACCAACA TGTGAGCAGG 
ATAACACAAG AAGACATTAA GAAGACACAT GGTGGGTCTT CAGGAAGCAG AGGCTACTAC 
TCCAGTGCTT TTGCAAGCTC AACAAATGCA TATATGCTGA TATACAGACT GAAGGATCCA 
ACAAGAAATG CAAAGTTTCT AGAAGTGGAC GAGTACCCAG AACACATTAA GAATCTGGTG 
CAGAAGGAGA GAGAGCTGGA AGAGCAGGAA AAGAGGCAAC GAGAGATTGA ACGCAACACG 
TGCAAGATAA AATTATTTTG TTTGCATCCT GTGAAACAAG TAATGATGGA AAACAAGCTG 
GAGGTTCATA AGGATAAGAC GCTAAAGGAA GCCGTTGAAA TGGCTTATAA GATGATGGAT 
TTAGAAGATG TGATTCCCTT GGATTGCTGT CGCCTGGTAA AATATGACGA GTTTCATGAT 
TACCTGGAAC GATCGTATGA GGGAGAAGAA GATACACCAA TGGGGTTGCT TCTAGGAGGA 
GTCAAGTCCA CATACATGTT TGATCTGCTG TTGGAAACGA GAAAGCCTGA TCAAATTTTT 
CAGTCTTACA AACCTGGAGA AGTGATGGTG AAAGTTCATG TTGTTGATCT GAAAGCAGAG 
ACTGTCGCTG CTCCTGTAAC AGTGCGTGCG TATTTAAATC AGACAGTTAC AGAGTTTAAA 
CAGCTGATCT CAAAGGCCAC GCACTTACCT GCAGACTCCA TGAGGATAGT GCTGGAGCGC 
TGCTACAATG ACCTGCGCCT TCTCAGCATG CCCAGCAAGA CCCTGAAGGC TGAGGGCTTC 
TTCAGAAGTA ACAAGGTGTT TGTTGAAAGC TCTGAGACAG TGGATCACCA GGCAGCCTTT 
ACAGACTCCC ACTTGTGGAA ACTCCTGGAT CGACATGCAA ATACAATCCG GTTATTTGTT 
TTGCTGCCTG AACAATCTCC AGGATCATAT TCGAAAAGGA CAGCATACCA GAAAGCTGGA 
GGTGATTCTG GTAATGTGGA CGATGATTGT GAAAGAGTCA AAGGACCTGC AGGGAATTTA 
AAGTCTGTGG ATGCTATTCT AGAAGAGAGC ACTGAAAAAC TGAAAAGCTT GTCACTGCAG 
CAGCAGCAGC AAGATGGGGA CAACGGAGAC AGCAGCAAAA GCACTGAGAC AAGTGACTTT 
GAAAATATTG AATCCCCTCT CAATGAGAGG GGCTCTTCCA CCTCTGTGGA TAATCGAGAA 
CTTGAACAGC ACATTCAGAC TTCTGACCCA GAAAACTTTC AGTCTGAGGA ACGCTCGGAC 
TCAGATGTGA ATAATGACCG GAGTACAAGT TCGGTAGACA GTGATATTCT TAGCTCCAGT 
CACAGCAGTG ACACTTTGTG CAATGCAGAC AGCGCTCAGA TTCCTCTGGC CAATGGACTT 
GACTCTCACA GTATCACAAG TAGTAGAAGA ACTAAAGCAA ACGAGGGGAA AAAGGAGACC 
TGGGACACAG CTGAAGAAGA CTCTGGGACT GACAGTGAAT ATGACGAGAG TGGCAAGAGC 
AGGGGGGACA TGCAGTACAT GTACTTCAAA GCTGACCCCT ACACTGCGGA TGAAGGCTCT 
GGGGAAGGAC ACAAATGGTT GATGGTGCAT GTTGATAAGA GAATCACCCT GGCAGCTTTC 
AAACAGCATT TAGAACCTTT TGTTGGAGTC CTCTCCTCTC ACTTCAAGGT TTTTCGAGTG 
TACACCAGCA ATCAAGAGTT TGAGACCGTC CGGTTGAATG AGACCCTTTC ATCATTTTCA 
GATGACAATA AGATCACAAT CCGACTGGGG AGAGCACTGA AGAAGGGAGA GTACAGAGTT 
AAAGTGTGCC AGCTCTTAGT CAATGAGCAA GAGCCATGCA AGTTTCTGCT AGACGCTGTG 
TTTGCTAAAG GCATGACTGT GCGGCAATCA AAAGAGGAGC TGATTCCTCA ACTCAGGGAG 
CAGTGTGGCT TAGACCTCAG CATCGACAGG TTTCGTCTAA GGAAGAAAAC ATGGAAGAAT 
CCTGGCACTG TCTTTTTGGA TTATCATATT TATGAAGAAG ATATTAATAT TTCCAGCAAC 
TGGGAAGTTT TCCTGGAAGT TCTTGATGGT GTGGAGAAGA TGAAGTCCAT GTCACAGCTT 
GCCATCTTAA CAAGACGCTG GAGGCCTGCA GAGATGAAGT TGGACCCCTT CCAGGAGCTT 
GTGTTGGAAA GCAATAGTGT TGATGAACTC CGAGAGAAGC TAAGTGAAAT CAGTGGGATT 
CCTTTGGAAG ACATTGAATT TGCCAAGGGT AGGGGAACCT TCCCCTGTGA TATTTCTGTC 
CTTGATATTC ATCAAGATTT GGACTGGAAT CCTAAAGTCT CTACCCTGAA TGTTTGGCCT 
CTTTATATCT GTGATGATGG TGCCGTCATA TTTTATAGGG ATAGAACAGA AGAAGTGATG 
GAATTGACAG ATGAGCAAAG AAATGAATTG ATGAAAAAGG AAAGCAGCCG ACTGCAGAAG 
ACGGGACATC GTGTGACATA CTCCCCTCGC AAAGAGAAAG CACTCAAGAT CTACCTGGAT 
GGGGCGCCAA ATAAAGATGT GGCTCAAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098904.1
CDS	119..4231
Translation	MKKKFLQIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKVEMAETDQRKDPIQFQLGEPNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	XM_030243044.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243044.1

ORF Insert Sequence:

ATGAAGAAGA AATTTTTACA GATAGAAAAT GCTGCTGAAG AACCTAGAGT CTTATGTATA 
ATACAAGACA CTACTAACTC GAAGACAGTG AGTGAACGGA TCACATTAAA TTTACCAGCA 
TCTACCCCAG TCCGAAAGCT CTTTGAAGAT GTGGCCAACA AAGTAGGCTA CATAAATGGA 
ACCTTTGATT TGACCAGGGA AAATGGAGTC ACTACTGCTG ACATGGCACC ACTGGATCAC 
ACCAGTGACA AGTCTCTTCT TGATGCTAAT TTTGAGCCAG GAAAGAAGAA CTTTCTGCAT 
TTGACAGATA AAGATGGTGA ACCGCCTCAG ATGTTGTTGG AGGACTCCAA TAACGTGGAT 
GACAGCGTGC ATGACAGGTT CATAGGTCCA CTGCCAAGAG AAGGTTCTGT GGCCTCTACC 
AATGATTATG TCAGCCAGAA CTACTCCTAT TCATCAATTT TGAACAAGTC AGAAACTGGT 
TATGTGGGAT TAGTAAACCA GGCGATGACG TGCTATTTGA ATAGCCTTTT GCAAACACTT 
TTTATGACTC CTGAATTTAG GAATGCATTA TATAAGTGGG AATTTGAAGA CTCTGAAGAA 
GATCCTGTAA CGAGTATCCC ATACCAGCTT CAAAGGCTTT TTGTTTTGTT ACAAACCAGC 
AAAAAGAGAG CAATTGAGAC CACAGATGTT ACAAGGAGCT TTGGATGGGA TAGCAGTGAG 
GCTTGGCAAC AGCATGATGT ACAAGAACTG TGCCGTGTTA TGTTTGATGC TTTGGAACAG 
AAATGGAAGC AAACAGAACA GGCTGATCTT ATAAATGAGC TGTACCAAGG CAAGCTGAAA 
GATTATGTGA GGTGTCTGGA ATGCGGTTAT GAAGGCTGGA GAATTGACAC GTATCTTGAT 
ATTCCACTGG TCATCCGACC TTATGGGTCC AGCCAAGCGT TTGCTAGTGT GGAAGAAGCG 
TTGCATGCAT TTATTCAACC AGAGATCTTG GATGGCCCAA ATCAATATTT TTGTGAACGT 
TGTAAGAAGA AGTGTGATGC TCGGAAGGGC CTTCGGTTTT TGCATTTCCC CTATCTGCTG 
ACCTTACAGT TGAAAAGGTT TGATTTTGAT TATACAACCA TGCATAGAAT TAAACTGAAT 
GATCGAATGT CATTTCCGGA GGAGCTGGAT ATGAGCACAT TTATTGATAT TGAAGATGAG 
AAATCTCCTC AGACTGAAAG TTGCACTGAT AGTGGAGCAG AAAATGAAGG CAGTTGCCAC 
AGCGACCAGA TGAGTAATGA TTTCTCCACG GATGATGCTG TTGATGAAGG GATATGCCTG 
GAGAGCAGTA GTGGGAGTGA GAAGATTTCA AAACCTGGCC TGGAAAAGGT AGAGATGGCG 
GAGACAGACC AGAGAAAGGA TCCCATCCAA TTCCAGCTTG GTGAACCAAA CTCCCTGATG 
TATGAGCTCT TCTCAGTGAT GGTGCATTCA GGGAGTGCTG CTGGAGGACA CTATTATGCT 
TGCATAAAGT CTTTCAGTGA CGATCAATGG TACAGTTTCA ATGACCAACA TGTGAGCAGG 
ATAACACAAG AAGACATTAA GAAGACACAT GGTGGGTCTT CAGGAAGCAG AGGCTACTAC 
TCCAGTGCTT TTGCAAGCTC AACAAATGCA TATATGCTGA TATACAGACT GAAGGATCCA 
ACAAGAAATG CAAAGTTTCT AGAAGTGGAC GAGTACCCAG AACACATTAA GAATCTGGTG 
CAGAAGGAGA GAGAGCTGGA AGAGCAGGAA AAGAGGCAAC GAGAGATTGA ACGCAACACG 
TGCAAGATAA AATTATTTTG TTTGCATCCT GTGAAACAAG TAATGATGGA AAACAAGCTG 
GAGGTTCATA AGGATAAGAC GCTAAAGGAA GCCGTTGAAA TGGCTTATAA GATGATGGAT 
TTAGAAGATG TGATTCCCTT GGATTGCTGT CGCCTGGTAA AATATGACGA GTTTCATGAT 
TACCTGGAAC GATCGTATGA GGGAGAAGAA GATACACCAA TGGGGTTGCT TCTAGGAGGA 
GTCAAGTCCA CATACATGTT TGATCTGCTG TTGGAAACGA GAAAGCCTGA TCAAATTTTT 
CAGTCTTACA AACCTGGAGA AGTGATGGTG AAAGTTCATG TTGTTGATCT GAAAGCAGAG 
ACTGTCGCTG CTCCTGTAAC AGTGCGTGCG TATTTAAATC AGACAGTTAC AGAGTTTAAA 
CAGCTGATCT CAAAGGCCAC GCACTTACCT GCAGACTCCA TGAGGATAGT GCTGGAGCGC 
TGCTACAATG ACCTGCGCCT TCTCAGCATG CCCAGCAAGA CCCTGAAGGC TGAGGGCTTC 
TTCAGAAGTA ACAAGGTGTT TGTTGAAAGC TCTGAGACAG TGGATCACCA GGCAGCCTTT 
ACAGACTCCC ACTTGTGGAA ACTCCTGGAT CGACATGCAA ATACAATCCG GTTATTTGTT 
TTGCTGCCTG AACAATCTCC AGGATCATAT TCGAAAAGGA CAGCATACCA GAAAGCTGGA 
GGTGATTCTG GTAATGTGGA CGATGATTGT GAAAGAGTCA AAGGACCTGC AGGGAATTTA 
AAGTCTGTGG ATGCTATTCT AGAAGAGAGC ACTGAAAAAC TGAAAAGCTT GTCACTGCAG 
CAGCAGCAGC AAGATGGGGA CAACGGAGAC AGCAGCAAAA GCACTGAGAC AAGTGACTTT 
GAAAATATTG AATCCCCTCT CAATGAGAGG GGCTCTTCCA CCTCTGTGGA TAATCGAGAA 
CTTGAACAGC ACATTCAGAC TTCTGACCCA GAAAACTTTC AGTCTGAGGA ACGCTCGGAC 
TCAGATGTGA ATAATGACCG GAGTACAAGT TCGGTAGACA GTGATATTCT TAGCTCCAGT 
CACAGCAGTG ACACTTTGTG CAATGCAGAC AGCGCTCAGA TTCCTCTGGC CAATGGACTT 
GACTCTCACA GTATCACAAG TAGTAGAAGA ACTAAAGCAA ACGAGGGGAA AAAGGAGACC 
TGGGACACAG CTGAAGAAGA CTCTGGGACT GACAGTGAAT ATGACGAGAG TGGCAAGAGC 
AGGGGGGACA TGCAGTACAT GTACTTCAAA GCTGACCCCT ACACTGCGGA TGAAGGCTCT 
GGGGAAGGAC ACAAATGGTT GATGGTGCAT GTTGATAAGA GAATCACCCT GGCAGCTTTC 
AAACAGCATT TAGAACCTTT TGTTGGAGTC CTCTCCTCTC ACTTCAAGGT TTTTCGAGTG 
TACACCAGCA ATCAAGAGTT TGAGACCGTC CGGTTGAATG AGACCCTTTC ATCATTTTCA 
GATGACAATA AGATCACAAT CCGACTGGGG AGAGCACTGA AGAAGGGAGA GTACAGAGTT 
AAAGTGTGCC AGCTCTTAGT CAATGAGCAA GAGCCATGCA AGTTTCTGCT AGACGCTGTG 
TTTGCTAAAG GCATGACTGT GCGGCAATCA AAAGAGGAGC TGATTCCTCA ACTCAGGGAG 
CAGTGTGGCT TAGACCTCAG CATCGACAGG TTTCGTCTAA GGAAGAAAAC ATGGAAGAAT 
CCTGGCACTG TCTTTTTGGA TTATCATATT TATGAAGAAG ATATTAATAT TTCCAGCAAC 
TGGGAAGTTT TCCTGGAAGT TCTTGATGGT GTGGAGAAGA TGAAGTCCAT GTCACAGCTT 
GCCATCTTAA CAAGACGCTG GAGGCCTGCA GAGATGAAGT TGGACCCCTT CCAGGAGCTT 
GTGTTGGAAA GCAATAGTGT TGATGAACTC CGAGAGAAGC TAAGTGAAAT CAGTGGGATT 
CCTTTGGAAG ACATTGAATT TGCCAAGGGT AGGGGAACCT TCCCCTGTGA TATTTCTGTC 
CTTGATATTC ATCAAGATTT GGACTGGAAT CCTAAAGTCT CTACCCTGAA TGTTTGGCCT 
CTTTATATCT GTGATGATGG TGCCGTCATA TTTTATAGGG ATAGAACAGA AGAAGTGATG 
GAATTGACAG ATGAGCAAAG AAATGAATTG ATGAAAAAGG AAAGCAGCCG ACTGCAGAAG 
ACGGGACATC GTGTGACATA CTCCCCTCGC AAAGAGAAAG CACTCAAGAT CTACCTGGAT 
GGGGCGCCAA ATAAAGATGT GGCTCAAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( NM_177249.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_796223.2 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 1, mRNA.

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CloneID	OMu06121
Clone ID Related Accession (Same CDS sequence)	NM_177249.3
Accession Version	NM_177249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY160489.1, AK082114.1, AK041541.1, AK131138.1, BC089364.1 and CO042533.1. On Nov 25, 2010 this sequence version replaced NM_177249.2. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode protein isoforms of identical length but with distinct amino acid sequences. ##Evidence-Data-START## Transcript exon combination :: BC108425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAAAATATCT TTGATGAAAT GAAGAAGAAA TTTTTACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796223.2
CDS	203..4333
Translation	MVPGEENQLVPKEDVFWRCRQNIFDEMKKKFLQIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	NM_177249.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177249.3

ORF Insert Sequence:

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAAAATATCT TTGATGAAAT GAAGAAGAAA TTTTTACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( NM_133758.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_598519.2 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 2, mRNA.

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CloneID	OMu10186
Clone ID Related Accession (Same CDS sequence)	NM_133758.3
Accession Version	NM_133758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY160489.1, AK041541.1, AK131138.1, BC089364.1 and CO042533.1. On Nov 25, 2010 this sequence version replaced NM_133758.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, which results in a shorter isoform (2), compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598519.2
CDS	203..4273
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	NM_133758.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133758.3

ORF Insert Sequence:

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( NM_001357952.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001344881.1 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 3, mRNA.

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CloneID	OMu39920
Clone ID Related Accession (Same CDS sequence)	NM_001357952.1 , XM_006508274.3
Accession Version	NM_001357952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122343.4. On Nov 10, 2017 this sequence version replaced XM_006508274.3. Transcript Variant: This variant (3) encodes isoform 3. Variants 1 and 3 encode protein isoforms of identical length but with distinct amino acid sequences. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.3652.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344881.1
CDS	203..4333
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKVEMAETDQRKDPIQFQLGEPNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	NM_001357952.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357952.1

ORF Insert Sequence:

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_006508273.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006508336.1 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X1, mRNA.

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CloneID	OMu39919
Clone ID Related Accession (Same CDS sequence)	XM_006508273.3 , XM_006508273.4
Accession Version	XM_006508273.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAAAATATCT TTGATGAAAT GAAGAAGAAA TTTTTACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508336.1
CDS	226..4416
Translation	MVPGEENQLVPKEDVFWRCRQNIFDEMKKKFLQIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKVEMAETDQRKDPIQFQLGEPNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	XM_006508273.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508273.3

ORF Insert Sequence:

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAAAATATCT TTGATGAAAT GAAGAAGAAA TTTTTACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_006508274.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006508337.1 Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X2, mRNA.

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CloneID	OMu39920
Clone ID Related Accession (Same CDS sequence)	NM_001357952.1 , XM_006508274.3
Accession Version	XM_006508274.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508274.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508337.1
CDS	225..4355
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKVEMAETDQRKDPIQFQLGEPNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD

Target ORF information:

RefSeq Version	XM_006508274.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin specific peptidase 47 (Usp47), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508274.3

ORF Insert Sequence:

ATGGTGCCCG GCGAGGAGAA CCAACTGGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTCT TATGTATAAT ACAAGACACT ACTAACTCGA AGACAGTGAG TGAACGGATC 
ACATTAAATT TACCAGCATC TACCCCAGTC CGAAAGCTCT TTGAAGATGT GGCCAACAAA 
GTAGGCTACA TAAATGGAAC CTTTGATTTG ACCAGGGAAA ATGGAGTCAC TACTGCTGAC 
ATGGCACCAC TGGATCACAC CAGTGACAAG TCTCTTCTTG ATGCTAATTT TGAGCCAGGA 
AAGAAGAACT TTCTGCATTT GACAGATAAA GATGGTGAAC CGCCTCAGAT GTTGTTGGAG 
GACTCCAATA ACGTGGATGA CAGCGTGCAT GACAGGTTCA TAGGTCCACT GCCAAGAGAA 
GGTTCTGTGG CCTCTACCAA TGATTATGTC AGCCAGAACT ACTCCTATTC ATCAATTTTG 
AACAAGTCAG AAACTGGTTA TGTGGGATTA GTAAACCAGG CGATGACGTG CTATTTGAAT 
AGCCTTTTGC AAACACTTTT TATGACTCCT GAATTTAGGA ATGCATTATA TAAGTGGGAA 
TTTGAAGACT CTGAAGAAGA TCCTGTAACG AGTATCCCAT ACCAGCTTCA AAGGCTTTTT 
GTTTTGTTAC AAACCAGCAA AAAGAGAGCA ATTGAGACCA CAGATGTTAC AAGGAGCTTT 
GGATGGGATA GCAGTGAGGC TTGGCAACAG CATGATGTAC AAGAACTGTG CCGTGTTATG 
TTTGATGCTT TGGAACAGAA ATGGAAGCAA ACAGAACAGG CTGATCTTAT AAATGAGCTG 
TACCAAGGCA AGCTGAAAGA TTATGTGAGG TGTCTGGAAT GCGGTTATGA AGGCTGGAGA 
ATTGACACGT ATCTTGATAT TCCACTGGTC ATCCGACCTT ATGGGTCCAG CCAAGCGTTT 
GCTAGTGTGG AAGAAGCGTT GCATGCATTT ATTCAACCAG AGATCTTGGA TGGCCCAAAT 
CAATATTTTT GTGAACGTTG TAAGAAGAAG TGTGATGCTC GGAAGGGCCT TCGGTTTTTG 
CATTTCCCCT ATCTGCTGAC CTTACAGTTG AAAAGGTTTG ATTTTGATTA TACAACCATG 
CATAGAATTA AACTGAATGA TCGAATGTCA TTTCCGGAGG AGCTGGATAT GAGCACATTT 
ATTGATATTG AAGATGAGAA ATCTCCTCAG ACTGAAAGTT GCACTGATAG TGGAGCAGAA 
AATGAAGGCA GTTGCCACAG CGACCAGATG AGTAATGATT TCTCCACGGA TGATGCTGTT 
GATGAAGGGA TATGCCTGGA GAGCAGTAGT GGGAGTGAGA AGATTTCAAA ACCTGGCCTG 
GAAAAGGTAG AGATGGCGGA GACAGACCAG AGAAAGGATC CCATCCAATT CCAGCTTGGT 
GAACCAAACT CCCTGATGTA TGAGCTCTTC TCAGTGATGG TGCATTCAGG GAGTGCTGCT 
GGAGGACACT ATTATGCTTG CATAAAGTCT TTCAGTGACG ATCAATGGTA CAGTTTCAAT 
GACCAACATG TGAGCAGGAT AACACAAGAA GACATTAAGA AGACACATGG TGGGTCTTCA 
GGAAGCAGAG GCTACTACTC CAGTGCTTTT GCAAGCTCAA CAAATGCATA TATGCTGATA 
TACAGACTGA AGGATCCAAC AAGAAATGCA AAGTTTCTAG AAGTGGACGA GTACCCAGAA 
CACATTAAGA ATCTGGTGCA GAAGGAGAGA GAGCTGGAAG AGCAGGAAAA GAGGCAACGA 
GAGATTGAAC GCAACACGTG CAAGATAAAA TTATTTTGTT TGCATCCTGT GAAACAAGTA 
ATGATGGAAA ACAAGCTGGA GGTTCATAAG GATAAGACGC TAAAGGAAGC CGTTGAAATG 
GCTTATAAGA TGATGGATTT AGAAGATGTG ATTCCCTTGG ATTGCTGTCG CCTGGTAAAA 
TATGACGAGT TTCATGATTA CCTGGAACGA TCGTATGAGG GAGAAGAAGA TACACCAATG 
GGGTTGCTTC TAGGAGGAGT CAAGTCCACA TACATGTTTG ATCTGCTGTT GGAAACGAGA 
AAGCCTGATC AAATTTTTCA GTCTTACAAA CCTGGAGAAG TGATGGTGAA AGTTCATGTT 
GTTGATCTGA AAGCAGAGAC TGTCGCTGCT CCTGTAACAG TGCGTGCGTA TTTAAATCAG 
ACAGTTACAG AGTTTAAACA GCTGATCTCA AAGGCCACGC ACTTACCTGC AGACTCCATG 
AGGATAGTGC TGGAGCGCTG CTACAATGAC CTGCGCCTTC TCAGCATGCC CAGCAAGACC 
CTGAAGGCTG AGGGCTTCTT CAGAAGTAAC AAGGTGTTTG TTGAAAGCTC TGAGACAGTG 
GATCACCAGG CAGCCTTTAC AGACTCCCAC TTGTGGAAAC TCCTGGATCG ACATGCAAAT 
ACAATCCGGT TATTTGTTTT GCTGCCTGAA CAATCTCCAG GATCATATTC GAAAAGGACA 
GCATACCAGA AAGCTGGAGG TGATTCTGGT AATGTGGACG ATGATTGTGA AAGAGTCAAA 
GGACCTGCAG GGAATTTAAA GTCTGTGGAT GCTATTCTAG AAGAGAGCAC TGAAAAACTG 
AAAAGCTTGT CACTGCAGCA GCAGCAGCAA GATGGGGACA ACGGAGACAG CAGCAAAAGC 
ACTGAGACAA GTGACTTTGA AAATATTGAA TCCCCTCTCA ATGAGAGGGG CTCTTCCACC 
TCTGTGGATA ATCGAGAACT TGAACAGCAC ATTCAGACTT CTGACCCAGA AAACTTTCAG 
TCTGAGGAAC GCTCGGACTC AGATGTGAAT AATGACCGGA GTACAAGTTC GGTAGACAGT 
GATATTCTTA GCTCCAGTCA CAGCAGTGAC ACTTTGTGCA ATGCAGACAG CGCTCAGATT 
CCTCTGGCCA ATGGACTTGA CTCTCACAGT ATCACAAGTA GTAGAAGAAC TAAAGCAAAC 
GAGGGGAAAA AGGAGACCTG GGACACAGCT GAAGAAGACT CTGGGACTGA CAGTGAATAT 
GACGAGAGTG GCAAGAGCAG GGGGGACATG CAGTACATGT ACTTCAAAGC TGACCCCTAC 
ACTGCGGATG AAGGCTCTGG GGAAGGACAC AAATGGTTGA TGGTGCATGT TGATAAGAGA 
ATCACCCTGG CAGCTTTCAA ACAGCATTTA GAACCTTTTG TTGGAGTCCT CTCCTCTCAC 
TTCAAGGTTT TTCGAGTGTA CACCAGCAAT CAAGAGTTTG AGACCGTCCG GTTGAATGAG 
ACCCTTTCAT CATTTTCAGA TGACAATAAG ATCACAATCC GACTGGGGAG AGCACTGAAG 
AAGGGAGAGT ACAGAGTTAA AGTGTGCCAG CTCTTAGTCA ATGAGCAAGA GCCATGCAAG 
TTTCTGCTAG ACGCTGTGTT TGCTAAAGGC ATGACTGTGC GGCAATCAAA AGAGGAGCTG 
ATTCCTCAAC TCAGGGAGCA GTGTGGCTTA GACCTCAGCA TCGACAGGTT TCGTCTAAGG 
AAGAAAACAT GGAAGAATCC TGGCACTGTC TTTTTGGATT ATCATATTTA TGAAGAAGAT 
ATTAATATTT CCAGCAACTG GGAAGTTTTC CTGGAAGTTC TTGATGGTGT GGAGAAGATG 
AAGTCCATGT CACAGCTTGC CATCTTAACA AGACGCTGGA GGCCTGCAGA GATGAAGTTG 
GACCCCTTCC AGGAGCTTGT GTTGGAAAGC AATAGTGTTG ATGAACTCCG AGAGAAGCTA 
AGTGAAATCA GTGGGATTCC TTTGGAAGAC ATTGAATTTG CCAAGGGTAG GGGAACCTTC 
CCCTGTGATA TTTCTGTCCT TGATATTCAT CAAGATTTGG ACTGGAATCC TAAAGTCTCT 
ACCCTGAATG TTTGGCCTCT TTATATCTGT GATGATGGTG CCGTCATATT TTATAGGGAT 
AGAACAGAAG AAGTGATGGA ATTGACAGAT GAGCAAAGAA ATGAATTGAT GAAAAAGGAA 
AGCAGCCGAC TGCAGAAGAC GGGACATCGT GTGACATACT CCCCTCGCAA AGAGAAAGCA 
CTCAAGATCT ACCTGGATGG GGCGCCAAAT AAAGATGTGG CTCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The ubiquitin-specific protease USP47 is a novel beta-TRCP interactor regulating cell survival.
Oncogene29(9)1384-93(2010 Mar)
Peschiaroli A,Skaar JR,Pagano M,Melino G

Phosphoproteomic analysis of the developing mouse brain.
Molecular & cellular proteomics : MCP3(11)1093-101(2004 Nov)
Ballif BA,Vill?n J,Beausoleil SA,Schwartz D,Gygi SP

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Kitamura H,Nakagawa T,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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USP47 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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