LOC109546527 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109546527
Entrez Gene ID	109546527
Full Name	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019917513.1	XP_019773072.1	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like isoform X1
XM_019917514.1	XP_019773073.1	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109546527 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109546527 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe35506	XM_019917513.1 Latest version!	Dendroctonus ponderosae (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like (LOC109546527), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODe35507	XM_019917514.1 Latest version!	Dendroctonus ponderosae (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like (LOC109546527), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109546527 ( XM_019917513.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019773072.1 Dendroctonus ponderosae (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like (LOC109546527), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe35506
Clone ID Related Accession (Same CDS sequence)	XM_019917513.1
Accession Version	XM_019917513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851160.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGG AGAAGGTAAA AGAATTTTAT GCCAACGATA TCGTGTATAG ATTTGATAAG 
AGCAAGAAAA TCATATTTGG GGTTGTGTTG GACTCCTATG AGGCTTCCAA TGATGATACA 
GATGATGTTA ATTCGTTACA AAAGGGCCAA ATCAGAGTCT CGTGGAGTAA CACTTCTAGG 
GAGCAAATTT GGCGTCAAAG TAAGGTTTAC TTGATGAATC GCAGTATTAT ACCAGGAGAT 
ATTATAAGAA GATTAGAGAA AGGCAAAGAG ACACAAAGGG GATACTGTAA AAGCAACAAA 
CAAATTGCCG CGGTACAAAT TGTAGGTACA GATAAGGTGA TTGAGCATGT CTCCAGTGAT 
CGGCTTAAAA ACGTTAGACC ATTTGATGTT GATGATGCTG TTAGTTGTGG AGATATATTT 
GGACGCATTG AAAGCATTGA CCAATTGGTC ACAATGCGAT CAATTGATGG TTCTGTAGTT 
GAACTTCTCA CCAGTATGAA TAATGATTTT GATGATTATT GGTTATCAAA GAGGGGTGGA 
TTGCTTTCAA TGGAAGTCTA CTATCCAGGT CAGGAAGTGG TCGGTGTTCC TGCCAATTTT 
GTCCGTCCCA CCTGGATTAA AAAAACAAAG ACCATGAAAC GGAATATGCA ACAAAGACAA 
AGATTCACTA TTCAGGAGGT CAATGATGCC ATGATAGAGG TTGCTTTGCA CAGTAATATT 
AGTGGTTATT CTCATATTTC AGAAATAATG AATGAGGATA TCAAAAAGCT GCAAGTTTTG 
GAACATCCAA GCGATACCGC TCTGGAATTA ATGGATAGGC GATTGTTGAA GCTTAATTCC 
CATGATATTC TTCTGAGAAA AAAGGACTGG GTACGGAAAT TGTCAATTAT GCATCGTCCG 
GAGGCGATTC GAGTTCGCCA CAATCTCAAT TGCAATTCGA AAAACACTAT TCGAGTGCCT 
CGTATTCGTA GCTCGTCGCT GCTGTTTTCT CCATCACCTA CCAAAATTGA CTGTGATAAA 
CCTCCAGTGT TTCCAGGCGT TGACGATGCC GAAGACGATT GGTGGACGGA AGAAGGTGAA 
GAATCTGAAG AGGAGAATAC TGAGTGTGGA AGTAACCCTT CAAGTAAAGC GACTAGCGTA 
GTAATGCCCA AACCTGTTCC AAGAAAGAAA CATTATCCAC CTAGACCCTG TGAACTTACT 
GCTGGAAACA CATTGGCAGT TGAAGTGGTT TCTGTTGAAT CAAAAATAAC GGTCGTATGG 
CAGGATGGCA CTGAAGAAAA AGATATTCCG TCCACCCAAC TGTATTACAA TATATCCATA 
GATGATCATG AGTTCTTCCC TGGTGAGTGG GTTGTGAAAT CCTCAGCCGG TAAAGATTCA 
TTTGGCGACG GTAAACATGG TGTTATTCAA CGAGTAGATT ATATGGAGAG AACTGCCACA 
GTTAAATGGT TTCAAACTTC GCAAGCTGGA AAGCCGCCGC TATTTATTAA TAGTGAAGAG 
TTGAGCGTAT ACGATTTAAA AAAACATTTT AAATATATAT ACAGGCCTGG CAGTATAGTG 
AAAGCCAACC CTTCCGTGGA TGGAAAGATA GGAAAAGTTA TTGATAGTTG CATAGATGGG 
TATGTGATCG TTGAATGGCA ACAGGGGAAG TTAGAGAATT GTTGGCCGGA ATCCATCGAG 
CTTCTTCCAG ATAGGTCGGA TTACGCGTAC TCGGATTCCG ATGAATCAAC AGCTGACTTA 
GTTTCATGGG AAACTGAAAG TATACAATCA TATGCAGGCG GCGATCTGAG CGATGAAACA 
GTATTACAAA ACATCGCAGC AAGATTAGAT TTTGTCCGAT CTAGAATCGT CTATCTAAAA 
GATGTATTTA AAACTCACAC CATTTCAGAT AGTTTAGCTA TTCTCAAAGA TTTACTTACG 
GTGTATGAAA ATTCAAGTTA TTTAGATAAA TTATTAGGAA CGTCTTTTTT TAGCTTGAAG 
AGCTCACATT TTCAGGCCTT GTTGATGCAA GCCAAAGAAA AAGCTAAAGC ACAAAACATC 
GAACTTAGAG GTCGATTATT TTCAATTGAA AATGGAGTCA ATGTTACGAA GAACAAATCA 
GCAGAATCGG AAAATATAAA GAAAATGATT AAATTGGAAA ACAAAGTAAA TGCGGAAATA 
GAGAAACAAG GAGCAAATAA ACAACTCAAA GATTCGACAG CGCCGTCGAC GCCCGAGAGT 
CTAGATGATT CCGACACTTG GCAAGAAAAT TTATGCGTGG AGCTTTTATC AACTTTAAAA 
CTAAGAATGG ATTTAACTTA TGCTGAAATA ATTAGCCGAA TAGGGGGCAG TCAAGCTCTA 
AGCATTATCA CAAAGGCAAC TGAAAGTATT GCCACACCTC TGATTTCCAC GCCTTTACCA 
AGCGTTCCTG CCACTCCAGA CGAATCTGTA TCTATAACAA ATTCCCTCAA AATTAAAAAT 
TCAACATCGA GTGACAAATT GATGGACGTT CAACTCGATA AAGGGGCTAA ATGGTATATC 
AGCGTAGAAG AAGCTCCTGT AACTCATCAC TATTTCACTT CTAAGTTTGA ACCAGGGGAT 
ACACAGAAAT TTCTCAAATC TGTCGTAAAA GAATACGCTT TGCTCAAAGA GTCTCTACCG 
GCCGATGTCT GGGTATGCTC TTATGGAAAC AGATTGGATC TCTTGAGTGT CATGATTCGG 
GGACCAGCTA AAACTCCTTA CGAAGATGGG TTGTTCTTGT TTGATATTCA GCTAAGTGCT 
GATTATCCCC GATCTCCTCC TTTGGTTCAT TACATCAGTT ACAGTTCAGA GCGACTAAAT 
CCTAATTTGT ATGTTGAAGG AAAAGTATGT GTTTCACTTT TGGGCACCTG GATGGGTAAA 
GGCACAGAAG TCTGGGGTCC AGACAGCACC CTTTTACAGC TCATTGTGTC CATTCAAGGC 
CTTATTTTAG TTTCTGAACC GTATTATAAT GAAGCTGGCT ATGAGAAGCA AACCGACTCT 
CAACAGGGAT ATGAAAACTC CAGAACCTAC AATGAACTCG TCATATTGAA GTTGGTTCAA 
AGTATGACTG AGCTGTTGCT TAATCCACCT GCCGTGTTTC ACGAACAAGT ACTGAGTCAT 
TTCGCACAAA ATGGGGAAAA ACTATGCCAA CGATTAGAGA CCTTCTGGAG AGATCAGGAT 
CCTCTAAAGC CCGATTTTCC TCTATTGCCT GTTAGCAAAG GCCTAATATT GTCACTATCA 
TCGGCTATCA AGCATTTTAG GGATGTCCTA GAACGTAAAA CGAGTTCAAA ATCGTCCGAA 
GCTAATCCTG TTTCCAGCAG TGAACAAAAG ATCGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019773072.1
CDS	723..4064
Translation	MSQEKVKEFYANDIVYRFDKSKKIIFGVVLDSYEASNDDTDDVNSLQKGQIRVSWSNTSREQIWRQSKVYLMNRSIIPGDIIRRLEKGKETQRGYCKSNKQIAAVQIVGTDKVIEHVSSDRLKNVRPFDVDDAVSCGDIFGRIESIDQLVTMRSIDGSVVELLTSMNNDFDDYWLSKRGGLLSMEVYYPGQEVVGVPANFVRPTWIKKTKTMKRNMQQRQRFTIQEVNDAMIEVALHSNISGYSHISEIMNEDIKKLQVLEHPSDTALELMDRRLLKLNSHDILLRKKDWVRKLSIMHRPEAIRVRHNLNCNSKNTIRVPRIRSSSLLFSPSPTKIDCDKPPVFPGVDDAEDDWWTEEGEESEEENTECGSNPSSKATSVVMPKPVPRKKHYPPRPCELTAGNTLAVEVVSVESKITVVWQDGTEEKDIPSTQLYYNISIDDHEFFPGEWVVKSSAGKDSFGDGKHGVIQRVDYMERTATVKWFQTSQAGKPPLFINSEELSVYDLKKHFKYIYRPGSIVKANPSVDGKIGKVIDSCIDGYVIVEWQQGKLENCWPESIELLPDRSDYAYSDSDESTADLVSWETESIQSYAGGDLSDETVLQNIAARLDFVRSRIVYLKDVFKTHTISDSLAILKDLLTVYENSSYLDKLLGTSFFSLKSSHFQALLMQAKEKAKAQNIELRGRLFSIENGVNVTKNKSAESENIKKMIKLENKVNAEIEKQGANKQLKDSTAPSTPESLDDSDTWQENLCVELLSTLKLRMDLTYAEIISRIGGSQALSIITKATESIATPLISTPLPSVPATPDESVSITNSLKIKNSTSSDKLMDVQLDKGAKWYISVEEAPVTHHYFTSKFEPGDTQKFLKSVVKEYALLKESLPADVWVCSYGNRLDLLSVMIRGPAKTPYEDGLFLFDIQLSADYPRSPPLVHYISYSSERLNPNLYVEGKVCVSLLGTWMGKGTEVWGPDSTLLQLIVSIQGLILVSEPYYNEAGYEKQTDSQQGYENSRTYNELVILKLVQSMTELLLNPPAVFHEQVLSHFAQNGEKLCQRLETFWRDQDPLKPDFPLLPVSKGLILSLSSAIKHFRDVLERKTSSKSSEANPVSSSEQKIEL

Target ORF information:

RefSeq Version	XM_019917513.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like (LOC109546527), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917513.1

ORF Insert Sequence:

ATGTCACAGG AGAAGGTAAA AGAATTTTAT GCCAACGATA TCGTGTATAG ATTTGATAAG 
AGCAAGAAAA TCATATTTGG GGTTGTGTTG GACTCCTATG AGGCTTCCAA TGATGATACA 
GATGATGTTA ATTCGTTACA AAAGGGCCAA ATCAGAGTCT CGTGGAGTAA CACTTCTAGG 
GAGCAAATTT GGCGTCAAAG TAAGGTTTAC TTGATGAATC GCAGTATTAT ACCAGGAGAT 
ATTATAAGAA GATTAGAGAA AGGCAAAGAG ACACAAAGGG GATACTGTAA AAGCAACAAA 
CAAATTGCCG CGGTACAAAT TGTAGGTACA GATAAGGTGA TTGAGCATGT CTCCAGTGAT 
CGGCTTAAAA ACGTTAGACC ATTTGATGTT GATGATGCTG TTAGTTGTGG AGATATATTT 
GGACGCATTG AAAGCATTGA CCAATTGGTC ACAATGCGAT CAATTGATGG TTCTGTAGTT 
GAACTTCTCA CCAGTATGAA TAATGATTTT GATGATTATT GGTTATCAAA GAGGGGTGGA 
TTGCTTTCAA TGGAAGTCTA CTATCCAGGT CAGGAAGTGG TCGGTGTTCC TGCCAATTTT 
GTCCGTCCCA CCTGGATTAA AAAAACAAAG ACCATGAAAC GGAATATGCA ACAAAGACAA 
AGATTCACTA TTCAGGAGGT CAATGATGCC ATGATAGAGG TTGCTTTGCA CAGTAATATT 
AGTGGTTATT CTCATATTTC AGAAATAATG AATGAGGATA TCAAAAAGCT GCAAGTTTTG 
GAACATCCAA GCGATACCGC TCTGGAATTA ATGGATAGGC GATTGTTGAA GCTTAATTCC 
CATGATATTC TTCTGAGAAA AAAGGACTGG GTACGGAAAT TGTCAATTAT GCATCGTCCG 
GAGGCGATTC GAGTTCGCCA CAATCTCAAT TGCAATTCGA AAAACACTAT TCGAGTGCCT 
CGTATTCGTA GCTCGTCGCT GCTGTTTTCT CCATCACCTA CCAAAATTGA CTGTGATAAA 
CCTCCAGTGT TTCCAGGCGT TGACGATGCC GAAGACGATT GGTGGACGGA AGAAGGTGAA 
GAATCTGAAG AGGAGAATAC TGAGTGTGGA AGTAACCCTT CAAGTAAAGC GACTAGCGTA 
GTAATGCCCA AACCTGTTCC AAGAAAGAAA CATTATCCAC CTAGACCCTG TGAACTTACT 
GCTGGAAACA CATTGGCAGT TGAAGTGGTT TCTGTTGAAT CAAAAATAAC GGTCGTATGG 
CAGGATGGCA CTGAAGAAAA AGATATTCCG TCCACCCAAC TGTATTACAA TATATCCATA 
GATGATCATG AGTTCTTCCC TGGTGAGTGG GTTGTGAAAT CCTCAGCCGG TAAAGATTCA 
TTTGGCGACG GTAAACATGG TGTTATTCAA CGAGTAGATT ATATGGAGAG AACTGCCACA 
GTTAAATGGT TTCAAACTTC GCAAGCTGGA AAGCCGCCGC TATTTATTAA TAGTGAAGAG 
TTGAGCGTAT ACGATTTAAA AAAACATTTT AAATATATAT ACAGGCCTGG CAGTATAGTG 
AAAGCCAACC CTTCCGTGGA TGGAAAGATA GGAAAAGTTA TTGATAGTTG CATAGATGGG 
TATGTGATCG TTGAATGGCA ACAGGGGAAG TTAGAGAATT GTTGGCCGGA ATCCATCGAG 
CTTCTTCCAG ATAGGTCGGA TTACGCGTAC TCGGATTCCG ATGAATCAAC AGCTGACTTA 
GTTTCATGGG AAACTGAAAG TATACAATCA TATGCAGGCG GCGATCTGAG CGATGAAACA 
GTATTACAAA ACATCGCAGC AAGATTAGAT TTTGTCCGAT CTAGAATCGT CTATCTAAAA 
GATGTATTTA AAACTCACAC CATTTCAGAT AGTTTAGCTA TTCTCAAAGA TTTACTTACG 
GTGTATGAAA ATTCAAGTTA TTTAGATAAA TTATTAGGAA CGTCTTTTTT TAGCTTGAAG 
AGCTCACATT TTCAGGCCTT GTTGATGCAA GCCAAAGAAA AAGCTAAAGC ACAAAACATC 
GAACTTAGAG GTCGATTATT TTCAATTGAA AATGGAGTCA ATGTTACGAA GAACAAATCA 
GCAGAATCGG AAAATATAAA GAAAATGATT AAATTGGAAA ACAAAGTAAA TGCGGAAATA 
GAGAAACAAG GAGCAAATAA ACAACTCAAA GATTCGACAG CGCCGTCGAC GCCCGAGAGT 
CTAGATGATT CCGACACTTG GCAAGAAAAT TTATGCGTGG AGCTTTTATC AACTTTAAAA 
CTAAGAATGG ATTTAACTTA TGCTGAAATA ATTAGCCGAA TAGGGGGCAG TCAAGCTCTA 
AGCATTATCA CAAAGGCAAC TGAAAGTATT GCCACACCTC TGATTTCCAC GCCTTTACCA 
AGCGTTCCTG CCACTCCAGA CGAATCTGTA TCTATAACAA ATTCCCTCAA AATTAAAAAT 
TCAACATCGA GTGACAAATT GATGGACGTT CAACTCGATA AAGGGGCTAA ATGGTATATC 
AGCGTAGAAG AAGCTCCTGT AACTCATCAC TATTTCACTT CTAAGTTTGA ACCAGGGGAT 
ACACAGAAAT TTCTCAAATC TGTCGTAAAA GAATACGCTT TGCTCAAAGA GTCTCTACCG 
GCCGATGTCT GGGTATGCTC TTATGGAAAC AGATTGGATC TCTTGAGTGT CATGATTCGG 
GGACCAGCTA AAACTCCTTA CGAAGATGGG TTGTTCTTGT TTGATATTCA GCTAAGTGCT 
GATTATCCCC GATCTCCTCC TTTGGTTCAT TACATCAGTT ACAGTTCAGA GCGACTAAAT 
CCTAATTTGT ATGTTGAAGG AAAAGTATGT GTTTCACTTT TGGGCACCTG GATGGGTAAA 
GGCACAGAAG TCTGGGGTCC AGACAGCACC CTTTTACAGC TCATTGTGTC CATTCAAGGC 
CTTATTTTAG TTTCTGAACC GTATTATAAT GAAGCTGGCT ATGAGAAGCA AACCGACTCT 
CAACAGGGAT ATGAAAACTC CAGAACCTAC AATGAACTCG TCATATTGAA GTTGGTTCAA 
AGTATGACTG AGCTGTTGCT TAATCCACCT GCCGTGTTTC ACGAACAAGT ACTGAGTCAT 
TTCGCACAAA ATGGGGAAAA ACTATGCCAA CGATTAGAGA CCTTCTGGAG AGATCAGGAT 
CCTCTAAAGC CCGATTTTCC TCTATTGCCT GTTAGCAAAG GCCTAATATT GTCACTATCA 
TCGGCTATCA AGCATTTTAG GGATGTCCTA GAACGTAAAA CGAGTTCAAA ATCGTCCGAA 
GCTAATCCTG TTTCCAGCAG TGAACAAAAG ATCGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109546527 ( XM_019917514.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019773073.1 Dendroctonus ponderosae (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like (LOC109546527), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe35507
Clone ID Related Accession (Same CDS sequence)	XM_019917514.1
Accession Version	XM_019917514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851160.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTC GAAGGAGTAA GAGATTGGTT TACTTGATGA ATCGCAGTAT TATACCAGGA 
GATATTATAA GAAGATTAGA GAAAGGCAAA GAGACACAAA GGGGATACTG TAAAAGCAAC 
AAACAAATTG CCGCGGTACA AATTGTAGGT ACAGATAAGG TGATTGAGCA TGTCTCCAGT 
GATCGGCTTA AAAACGTTAG ACCATTTGAT GTTGATGATG CTGTTAGTTG TGGAGATATA 
TTTGGACGCA TTGAAAGCAT TGACCAATTG GTCACAATGC GATCAATTGA TGGTTCTGTA 
GTTGAACTTC TCACCAGTAT GAATAATGAT TTTGATGATT ATTGGTTATC AAAGAGGGGT 
GGATTGCTTT CAATGGAAGT CTACTATCCA GGTCAGGAAG TGGTCGGTGT TCCTGCCAAT 
TTTGTCCGTC CCACCTGGAT TAAAAAAACA AAGACCATGA AACGGAATAT GCAACAAAGA 
CAAAGATTCA CTATTCAGGA GGTCAATGAT GCCATGATAG AGGTTGCTTT GCACAGTAAT 
ATTAGTGGTT ATTCTCATAT TTCAGAAATA ATGAATGAGG ATATCAAAAA GCTGCAAGTT 
TTGGAACATC CAAGCGATAC CGCTCTGGAA TTAATGGATA GGCGATTGTT GAAGCTTAAT 
TCCCATGATA TTCTTCTGAG AAAAAAGGAC TGGGTACGGA AATTGTCAAT TATGCATCGT 
CCGGAGGCGA TTCGAGTTCG CCACAATCTC AATTGCAATT CGAAAAACAC TATTCGAGTG 
CCTCGTATTC GTAGCTCGTC GCTGCTGTTT TCTCCATCAC CTACCAAAAT TGACTGTGAT 
AAACCTCCAG TGTTTCCAGG CGTTGACGAT GCCGAAGACG ATTGGTGGAC GGAAGAAGGT 
GAAGAATCTG AAGAGGAGAA TACTGAGTGT GGAAGTAACC CTTCAAGTAA AGCGACTAGC 
GTAGTAATGC CCAAACCTGT TCCAAGAAAG AAACATTATC CACCTAGACC CTGTGAACTT 
ACTGCTGGAA ACACATTGGC AGTTGAAGTG GTTTCTGTTG AATCAAAAAT AACGGTCGTA 
TGGCAGGATG GCACTGAAGA AAAAGATATT CCGTCCACCC AACTGTATTA CAATATATCC 
ATAGATGATC ATGAGTTCTT CCCTGGTGAG TGGGTTGTGA AATCCTCAGC CGGTAAAGAT 
TCATTTGGCG ACGGTAAACA TGGTGTTATT CAACGAGTAG ATTATATGGA GAGAACTGCC 
ACAGTTAAAT GGTTTCAAAC TTCGCAAGCT GGAAAGCCGC CGCTATTTAT TAATAGTGAA 
GAGTTGAGCG TATACGATTT AAAAAAACAT TTTAAATATA TATACAGGCC TGGCAGTATA 
GTGAAAGCCA ACCCTTCCGT GGATGGAAAG ATAGGAAAAG TTATTGATAG TTGCATAGAT 
GGGTATGTGA TCGTTGAATG GCAACAGGGG AAGTTAGAGA ATTGTTGGCC GGAATCCATC 
GAGCTTCTTC CAGATAGGTC GGATTACGCG TACTCGGATT CCGATGAATC AACAGCTGAC 
TTAGTTTCAT GGGAAACTGA AAGTATACAA TCATATGCAG GCGGCGATCT GAGCGATGAA 
ACAGTATTAC AAAACATCGC AGCAAGATTA GATTTTGTCC GATCTAGAAT CGTCTATCTA 
AAAGATGTAT TTAAAACTCA CACCATTTCA GATAGTTTAG CTATTCTCAA AGATTTACTT 
ACGGTGTATG AAAATTCAAG TTATTTAGAT AAATTATTAG GAACGTCTTT TTTTAGCTTG 
AAGAGCTCAC ATTTTCAGGC CTTGTTGATG CAAGCCAAAG AAAAAGCTAA AGCACAAAAC 
ATCGAACTTA GAGGTCGATT ATTTTCAATT GAAAATGGAG TCAATGTTAC GAAGAACAAA 
TCAGCAGAAT CGGAAAATAT AAAGAAAATG ATTAAATTGG AAAACAAAGT AAATGCGGAA 
ATAGAGAAAC AAGGAGCAAA TAAACAACTC AAAGATTCGA CAGCGCCGTC GACGCCCGAG 
AGTCTAGATG ATTCCGACAC TTGGCAAGAA AATTTATGCG TGGAGCTTTT ATCAACTTTA 
AAACTAAGAA TGGATTTAAC TTATGCTGAA ATAATTAGCC GAATAGGGGG CAGTCAAGCT 
CTAAGCATTA TCACAAAGGC AACTGAAAGT ATTGCCACAC CTCTGATTTC CACGCCTTTA 
CCAAGCGTTC CTGCCACTCC AGACGAATCT GTATCTATAA CAAATTCCCT CAAAATTAAA 
AATTCAACAT CGAGTGACAA ATTGATGGAC GTTCAACTCG ATAAAGGGGC TAAATGGTAT 
ATCAGCGTAG AAGAAGCTCC TGTAACTCAT CACTATTTCA CTTCTAAGTT TGAACCAGGG 
GATACACAGA AATTTCTCAA ATCTGTCGTA AAAGAATACG CTTTGCTCAA AGAGTCTCTA 
CCGGCCGATG TCTGGGTATG CTCTTATGGA AACAGATTGG ATCTCTTGAG TGTCATGATT 
CGGGGACCAG CTAAAACTCC TTACGAAGAT GGGTTGTTCT TGTTTGATAT TCAGCTAAGT 
GCTGATTATC CCCGATCTCC TCCTTTGGTT CATTACATCA GTTACAGTTC AGAGCGACTA 
AATCCTAATT TGTATGTTGA AGGAAAAGTA TGTGTTTCAC TTTTGGGCAC CTGGATGGGT 
AAAGGCACAG AAGTCTGGGG TCCAGACAGC ACCCTTTTAC AGCTCATTGT GTCCATTCAA 
GGCCTTATTT TAGTTTCTGA ACCGTATTAT AATGAAGCTG GCTATGAGAA GCAAACCGAC 
TCTCAACAGG GATATGAAAA CTCCAGAACC TACAATGAAC TCGTCATATT GAAGTTGGTT 
CAAAGTATGA CTGAGCTGTT GCTTAATCCA CCTGCCGTGT TTCACGAACA AGTACTGAGT 
CATTTCGCAC AAAATGGGGA AAAACTATGC CAACGATTAG AGACCTTCTG GAGAGATCAG 
GATCCTCTAA AGCCCGATTT TCCTCTATTG CCTGTTAGCA AAGGCCTAAT ATTGTCACTA 
TCATCGGCTA TCAAGCATTT TAGGGATGTC CTAGAACGTA AAACGAGTTC AAAATCGTCC 
GAAGCTAATC CTGTTTCCAG CAGTGAACAA AAGATCGAAT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019773073.1
CDS	599..3763
Translation	MSPRRSKRLVYLMNRSIIPGDIIRRLEKGKETQRGYCKSNKQIAAVQIVGTDKVIEHVSSDRLKNVRPFDVDDAVSCGDIFGRIESIDQLVTMRSIDGSVVELLTSMNNDFDDYWLSKRGGLLSMEVYYPGQEVVGVPANFVRPTWIKKTKTMKRNMQQRQRFTIQEVNDAMIEVALHSNISGYSHISEIMNEDIKKLQVLEHPSDTALELMDRRLLKLNSHDILLRKKDWVRKLSIMHRPEAIRVRHNLNCNSKNTIRVPRIRSSSLLFSPSPTKIDCDKPPVFPGVDDAEDDWWTEEGEESEEENTECGSNPSSKATSVVMPKPVPRKKHYPPRPCELTAGNTLAVEVVSVESKITVVWQDGTEEKDIPSTQLYYNISIDDHEFFPGEWVVKSSAGKDSFGDGKHGVIQRVDYMERTATVKWFQTSQAGKPPLFINSEELSVYDLKKHFKYIYRPGSIVKANPSVDGKIGKVIDSCIDGYVIVEWQQGKLENCWPESIELLPDRSDYAYSDSDESTADLVSWETESIQSYAGGDLSDETVLQNIAARLDFVRSRIVYLKDVFKTHTISDSLAILKDLLTVYENSSYLDKLLGTSFFSLKSSHFQALLMQAKEKAKAQNIELRGRLFSIENGVNVTKNKSAESENIKKMIKLENKVNAEIEKQGANKQLKDSTAPSTPESLDDSDTWQENLCVELLSTLKLRMDLTYAEIISRIGGSQALSIITKATESIATPLISTPLPSVPATPDESVSITNSLKIKNSTSSDKLMDVQLDKGAKWYISVEEAPVTHHYFTSKFEPGDTQKFLKSVVKEYALLKESLPADVWVCSYGNRLDLLSVMIRGPAKTPYEDGLFLFDIQLSADYPRSPPLVHYISYSSERLNPNLYVEGKVCVSLLGTWMGKGTEVWGPDSTLLQLIVSIQGLILVSEPYYNEAGYEKQTDSQQGYENSRTYNELVILKLVQSMTELLLNPPAVFHEQVLSHFAQNGEKLCQRLETFWRDQDPLKPDFPLLPVSKGLILSLSSAIKHFRDVLERKTSSKSSEANPVSSSEQKIEL

Target ORF information:

RefSeq Version	XM_019917514.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O-like (LOC109546527), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917514.1

ORF Insert Sequence:

ATGTCTCCTC GAAGGAGTAA GAGATTGGTT TACTTGATGA ATCGCAGTAT TATACCAGGA 
GATATTATAA GAAGATTAGA GAAAGGCAAA GAGACACAAA GGGGATACTG TAAAAGCAAC 
AAACAAATTG CCGCGGTACA AATTGTAGGT ACAGATAAGG TGATTGAGCA TGTCTCCAGT 
GATCGGCTTA AAAACGTTAG ACCATTTGAT GTTGATGATG CTGTTAGTTG TGGAGATATA 
TTTGGACGCA TTGAAAGCAT TGACCAATTG GTCACAATGC GATCAATTGA TGGTTCTGTA 
GTTGAACTTC TCACCAGTAT GAATAATGAT TTTGATGATT ATTGGTTATC AAAGAGGGGT 
GGATTGCTTT CAATGGAAGT CTACTATCCA GGTCAGGAAG TGGTCGGTGT TCCTGCCAAT 
TTTGTCCGTC CCACCTGGAT TAAAAAAACA AAGACCATGA AACGGAATAT GCAACAAAGA 
CAAAGATTCA CTATTCAGGA GGTCAATGAT GCCATGATAG AGGTTGCTTT GCACAGTAAT 
ATTAGTGGTT ATTCTCATAT TTCAGAAATA ATGAATGAGG ATATCAAAAA GCTGCAAGTT 
TTGGAACATC CAAGCGATAC CGCTCTGGAA TTAATGGATA GGCGATTGTT GAAGCTTAAT 
TCCCATGATA TTCTTCTGAG AAAAAAGGAC TGGGTACGGA AATTGTCAAT TATGCATCGT 
CCGGAGGCGA TTCGAGTTCG CCACAATCTC AATTGCAATT CGAAAAACAC TATTCGAGTG 
CCTCGTATTC GTAGCTCGTC GCTGCTGTTT TCTCCATCAC CTACCAAAAT TGACTGTGAT 
AAACCTCCAG TGTTTCCAGG CGTTGACGAT GCCGAAGACG ATTGGTGGAC GGAAGAAGGT 
GAAGAATCTG AAGAGGAGAA TACTGAGTGT GGAAGTAACC CTTCAAGTAA AGCGACTAGC 
GTAGTAATGC CCAAACCTGT TCCAAGAAAG AAACATTATC CACCTAGACC CTGTGAACTT 
ACTGCTGGAA ACACATTGGC AGTTGAAGTG GTTTCTGTTG AATCAAAAAT AACGGTCGTA 
TGGCAGGATG GCACTGAAGA AAAAGATATT CCGTCCACCC AACTGTATTA CAATATATCC 
ATAGATGATC ATGAGTTCTT CCCTGGTGAG TGGGTTGTGA AATCCTCAGC CGGTAAAGAT 
TCATTTGGCG ACGGTAAACA TGGTGTTATT CAACGAGTAG ATTATATGGA GAGAACTGCC 
ACAGTTAAAT GGTTTCAAAC TTCGCAAGCT GGAAAGCCGC CGCTATTTAT TAATAGTGAA 
GAGTTGAGCG TATACGATTT AAAAAAACAT TTTAAATATA TATACAGGCC TGGCAGTATA 
GTGAAAGCCA ACCCTTCCGT GGATGGAAAG ATAGGAAAAG TTATTGATAG TTGCATAGAT 
GGGTATGTGA TCGTTGAATG GCAACAGGGG AAGTTAGAGA ATTGTTGGCC GGAATCCATC 
GAGCTTCTTC CAGATAGGTC GGATTACGCG TACTCGGATT CCGATGAATC AACAGCTGAC 
TTAGTTTCAT GGGAAACTGA AAGTATACAA TCATATGCAG GCGGCGATCT GAGCGATGAA 
ACAGTATTAC AAAACATCGC AGCAAGATTA GATTTTGTCC GATCTAGAAT CGTCTATCTA 
AAAGATGTAT TTAAAACTCA CACCATTTCA GATAGTTTAG CTATTCTCAA AGATTTACTT 
ACGGTGTATG AAAATTCAAG TTATTTAGAT AAATTATTAG GAACGTCTTT TTTTAGCTTG 
AAGAGCTCAC ATTTTCAGGC CTTGTTGATG CAAGCCAAAG AAAAAGCTAA AGCACAAAAC 
ATCGAACTTA GAGGTCGATT ATTTTCAATT GAAAATGGAG TCAATGTTAC GAAGAACAAA 
TCAGCAGAAT CGGAAAATAT AAAGAAAATG ATTAAATTGG AAAACAAAGT AAATGCGGAA 
ATAGAGAAAC AAGGAGCAAA TAAACAACTC AAAGATTCGA CAGCGCCGTC GACGCCCGAG 
AGTCTAGATG ATTCCGACAC TTGGCAAGAA AATTTATGCG TGGAGCTTTT ATCAACTTTA 
AAACTAAGAA TGGATTTAAC TTATGCTGAA ATAATTAGCC GAATAGGGGG CAGTCAAGCT 
CTAAGCATTA TCACAAAGGC AACTGAAAGT ATTGCCACAC CTCTGATTTC CACGCCTTTA 
CCAAGCGTTC CTGCCACTCC AGACGAATCT GTATCTATAA CAAATTCCCT CAAAATTAAA 
AATTCAACAT CGAGTGACAA ATTGATGGAC GTTCAACTCG ATAAAGGGGC TAAATGGTAT 
ATCAGCGTAG AAGAAGCTCC TGTAACTCAT CACTATTTCA CTTCTAAGTT TGAACCAGGG 
GATACACAGA AATTTCTCAA ATCTGTCGTA AAAGAATACG CTTTGCTCAA AGAGTCTCTA 
CCGGCCGATG TCTGGGTATG CTCTTATGGA AACAGATTGG ATCTCTTGAG TGTCATGATT 
CGGGGACCAG CTAAAACTCC TTACGAAGAT GGGTTGTTCT TGTTTGATAT TCAGCTAAGT 
GCTGATTATC CCCGATCTCC TCCTTTGGTT CATTACATCA GTTACAGTTC AGAGCGACTA 
AATCCTAATT TGTATGTTGA AGGAAAAGTA TGTGTTTCAC TTTTGGGCAC CTGGATGGGT 
AAAGGCACAG AAGTCTGGGG TCCAGACAGC ACCCTTTTAC AGCTCATTGT GTCCATTCAA 
GGCCTTATTT TAGTTTCTGA ACCGTATTAT AATGAAGCTG GCTATGAGAA GCAAACCGAC 
TCTCAACAGG GATATGAAAA CTCCAGAACC TACAATGAAC TCGTCATATT GAAGTTGGTT 
CAAAGTATGA CTGAGCTGTT GCTTAATCCA CCTGCCGTGT TTCACGAACA AGTACTGAGT 
CATTTCGCAC AAAATGGGGA AAAACTATGC CAACGATTAG AGACCTTCTG GAGAGATCAG 
GATCCTCTAA AGCCCGATTT TCCTCTATTG CCTGTTAGCA AAGGCCTAAT ATTGTCACTA 
TCATCGGCTA TCAAGCATTT TAGGGATGTC CTAGAACGTA AAACGAGTTC AAAATCGTCC 
GAAGCTAATC CTGTTTCCAG CAGTGAACAA AAGATCGAAT TATAA