tmem259 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmem259
Entrez Gene ID	571445
Full Name	transmembrane protein 259
Synonyms	im:7154960
General protein information	Preferred Names transmembrane protein 259 Names membralin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155960.2	XP_005156017.1	membralin isoform X1
XM_695035.7	XP_700127.3	membralin isoform X2
XM_005155960.3	XP_005156017.1	membralin isoform X1
XM_021472449.1	XP_021328124.1	membralin isoform X3
XM_695035.8	XP_700127.3	membralin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMEM259	NP_001028198.1
Pan troglodytes (chimpanzee)	C19H19orf6	XP_003954544.1
Xenopus tropicalis (tropical clawed frog)	tmem259	NP_001072254.1
Bos taurus (cattle)	TMEM259	XP_002689215.2
Danio rerio (zebrafish)	tmem259	XP_700127.3
Canis lupus familiaris (dog)	C20H19orf6	XP_533963.2
Gallus gallus (chicken)	C19ORF6	XP_418222.2
Mus musculus (house mouse)	Tmem259	NP_001003949.2
Rattus norvegicus (Norway rat)	Mbrl	NP_001094356.1

Gene Ontology

The following tmem259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145275	XM_021472449.1 Latest version!	Danio rerio transmembrane protein 259 (tmem259), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa16915	XM_005155960.3 Latest version!	Danio rerio transmembrane protein 259 (tmem259), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa16916	XM_695035.8 Latest version!	Danio rerio transmembrane protein 259 (tmem259), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa16915	XM_005155960.2	Danio rerio transmembrane protein 259 (tmem259), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa16916	XM_695035.7	Danio rerio transmembrane protein 259 (tmem259), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmem259 ( XM_021472449.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328124.1 Danio rerio transmembrane protein 259 (tmem259), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa145275
Clone ID Related Accession (Same CDS sequence)	XM_021472449.1
Accession Version	XM_021472449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membralin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394806.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAG TGAGTTTATT ACATGGTTAT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328124.1
CDS	801..1346
Translation	MSDNQGNVNNNNVPLNNNNNGGPNRIRNPPMNQNPLINVRDRLFHALFFKMAVTYARLFPPSFRRIFEFLVLLKALFMLFILAYIHIAFSRSPINCLEHVREKWPRDGILRVEIQRNSSRAPIFLQFYETEGIQGLVKEPEEDGHENRQPVVPTDAEEEEEMTMDMFDNSTVRVSLLHGYF

Target ORF information:

RefSeq Version	XM_021472449.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 259 (tmem259), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472449.1

ORF Insert Sequence:

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAG TGAGTTTATT ACATGGTTAT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem259 ( XM_005155960.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156017.1 Danio rerio transmembrane protein 259 (tmem259), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16915
Clone ID Related Accession (Same CDS sequence)	XM_005155960.2 , XM_005155960.3
Accession Version	XM_005155960.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membralin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005155960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGGT ATGCAGCCGC TGAGGGAGAC TGTCTCCGAG 
ATTGAGATGC TAACTCGAGC AGTGTGGCCG CAAGAAGAGT ATATTGTTGA ATATTCTTTG 
GAATATGGTT TCCTAAGGCT ATCCCAGGCC ACACGTCAAC GACTCAACAT CCCTGTTATG 
GTGGTGACCC TTGATCCAAT GAAAGATCAG TGCTTTGGAG ATGGTTTCAG TCGCTTCCTC 
CTGGACGAGT GCCTGGGTTA TGATGATATT CTAATGTCCA GTGTGAAGGC ATTAGCAGAG 
AATGAGGAAA ACAAAGGCTT CCTCAGGAAC GTGGTGTCTG GAGAACACTA TCGTTTTGTC 
AGCATGTGGA TGGCTCGTAC CTCCTACCTG GCAGCTTTTG TAATAATGGT TATCTTTACT 
CTGTCAGTGT CTATGCTGCT GCGATACTCG CACCACCAAA TTTTTGTTTT TATAGTGGAT 
CTTCTTCAGA TGTTAGAGAT GAATATGACC ATTGCCTTCC CTGCTGCCCC ACTGCTCACT 
GTCATCCTGG CACTTGTGGG GATGGAGGCC ATCATGTCTG AATTTTTCAA TGACACAACC 
ACTGCCTTCT ACATCATCCT CATTGTTTGG TTGGCAGACC AATATGATGC CATCTGTTGC 
CACACCAATA CAAGCAAACG CCACTGGCTC AGATTCTTTT ATCTGTATCA CTTTGCCTTC 
TATGCATATC ATTACCGCTT TAACGGCCAG TACAGCAGTT TGGCTCTTGT CACATCTTGG 
CTCTTCATTC AGCACTCAAT GATCTATTTT TTCCATCACT ACGAGCTGCC GGCTATCTTG 
CAGCAAATCC GGATCCAGGA GATGCTGCTT CAGAACCAGC AGGTTGGTCA AGGAAACCAG 
ACTGCATTGC AGGACAATCT CAACAACAAC ACTAGCACCG CTGCACCAGC TGGCCAAGCT 
AACGCAGCTG CCAACGTGAA CGCTAATGGC CAGGACCAGC AAGCCGCTCA GGACCAACCT 
CTGCTTGAAA CCCACAGCGG CTTGGTGGTG AATCCCAGTC TATCTATCAA TCCATCTGGC 
TCTGATCTCG ACTGGATGGC TGAGATTGCC ATTGGCCCAG ACGTTCTGTC AGTTCCAAAC 
CTAGGTGACC CGCTTTTAGA ACCAGGTGGG ATCCAGGAAG CAACGTCATC CAGCGCAGTT 
GTAACAGAGC TTTGTGGTCC CAACACTGCA GGAGAGAACC AAAATGGGAA CATAAGCACA 
GCCAGCGTTC CTACGGTCAA CGCAAAAGGG AGGAGTTGCG CTCAGGGTCT GGGTCCTCCT 
GGTGGTGGAG GAGGACAGAA AGAATCTGAA ACTAACTCCA GTTCCTCCTC AGTGACCTCA 
CACACAGACT GCAGAGTAGT AGAGTCACGC CGAGGCGCAA CAGACTGGGA CGACAAGGTG 
GAGAATAAAA GTGAAAATTC GGACTCACAC TCCACCCCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156017.1
CDS	798..2882
Translation	MSDNQGNVNNNNVPLNNNNNGGPNRIRNPPMNQNPLINVRDRLFHALFFKMAVTYARLFPPSFRRIFEFLVLLKALFMLFILAYIHIAFSRSPINCLEHVREKWPRDGILRVEIQRNSSRAPIFLQFYETEGIQGLVKEPEEDGHENRQPVVPTDAEEEEEMTMDMFDNSTVRFELDIEPRLNPSMSGGVPGSSLNESQDLSFSQTASKGMQPLRETVSEIEMLTRAVWPQEEYIVEYSLEYGFLRLSQATRQRLNIPVMVVTLDPMKDQCFGDGFSRFLLDECLGYDDILMSSVKALAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMTIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQNQQVGQGNQTALQDNLNNNTSTAAPAGQANAAANVNANGQDQQAAQDQPLLETHSGLVVNPSLSINPSGSDLDWMAEIAIGPDVLSVPNLGDPLLEPGGIQEATSSSAVVTELCGPNTAGENQNGNISTASVPTVNAKGRSCAQGLGPPGGGGGQKESETNSSSSSVTSHTDCRVVESRRGATDWDDKVENKSENSDSHSTPA

Target ORF information:

RefSeq Version	XM_005155960.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 259 (tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155960.2

ORF Insert Sequence:

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGGT ATGCAGCCGC TGAGGGAGAC TGTCTCCGAG 
ATTGAGATGC TAACTCGAGC AGTGTGGCCG CAAGAAGAGT ATATTGTTGA ATATTCTTTG 
GAATATGGTT TCCTAAGGCT ATCCCAGGCC ACACGTCAAC GACTCAACAT CCCTGTTATG 
GTGGTGACCC TTGATCCAAT GAAAGATCAG TGCTTTGGAG ATGGTTTCAG TCGCTTCCTC 
CTGGACGAGT GCCTGGGTTA TGATGATATT CTAATGTCCA GTGTGAAGGC ATTAGCAGAG 
AATGAGGAAA ACAAAGGCTT CCTCAGGAAC GTGGTGTCTG GAGAACACTA TCGTTTTGTC 
AGCATGTGGA TGGCTCGTAC CTCCTACCTG GCAGCTTTTG TAATAATGGT TATCTTTACT 
CTGTCAGTGT CTATGCTGCT GCGATACTCG CACCACCAAA TTTTTGTTTT TATAGTGGAT 
CTTCTTCAGA TGTTAGAGAT GAATATGACC ATTGCCTTCC CTGCTGCCCC ACTGCTCACT 
GTCATCCTGG CACTTGTGGG GATGGAGGCC ATCATGTCTG AATTTTTCAA TGACACAACC 
ACTGCCTTCT ACATCATCCT CATTGTTTGG TTGGCAGACC AATATGATGC CATCTGTTGC 
CACACCAATA CAAGCAAACG CCACTGGCTC AGATTCTTTT ATCTGTATCA CTTTGCCTTC 
TATGCATATC ATTACCGCTT TAACGGCCAG TACAGCAGTT TGGCTCTTGT CACATCTTGG 
CTCTTCATTC AGCACTCAAT GATCTATTTT TTCCATCACT ACGAGCTGCC GGCTATCTTG 
CAGCAAATCC GGATCCAGGA GATGCTGCTT CAGAACCAGC AGGTTGGTCA AGGAAACCAG 
ACTGCATTGC AGGACAATCT CAACAACAAC ACTAGCACCG CTGCACCAGC TGGCCAAGCT 
AACGCAGCTG CCAACGTGAA CGCTAATGGC CAGGACCAGC AAGCCGCTCA GGACCAACCT 
CTGCTTGAAA CCCACAGCGG CTTGGTGGTG AATCCCAGTC TATCTATCAA TCCATCTGGC 
TCTGATCTCG ACTGGATGGC TGAGATTGCC ATTGGCCCAG ACGTTCTGTC AGTTCCAAAC 
CTAGGTGACC CGCTTTTAGA ACCAGGTGGG ATCCAGGAAG CAACGTCATC CAGCGCAGTT 
GTAACAGAGC TTTGTGGTCC CAACACTGCA GGAGAGAACC AAAATGGGAA CATAAGCACA 
GCCAGCGTTC CTACGGTCAA CGCAAAAGGG AGGAGTTGCG CTCAGGGTCT GGGTCCTCCT 
GGTGGTGGAG GAGGACAGAA AGAATCTGAA ACTAACTCCA GTTCCTCCTC AGTGACCTCA 
CACACAGACT GCAGAGTAGT AGAGTCACGC CGAGGCGCAA CAGACTGGGA CGACAAGGTG 
GAGAATAAAA GTGAAAATTC GGACTCACAC TCCACCCCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem259 ( XM_005155960.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156017.1 Danio rerio transmembrane protein 259 (tmem259), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16915
Clone ID Related Accession (Same CDS sequence)	XM_005155960.2 , XM_005155960.3
Accession Version	XM_005155960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGGT ATGCAGCCGC TGAGGGAGAC TGTCTCCGAG 
ATTGAGATGC TAACTCGAGC AGTGTGGCCG CAAGAAGAGT ATATTGTTGA ATATTCTTTG 
GAATATGGTT TCCTAAGGCT ATCCCAGGCC ACACGTCAAC GACTCAACAT CCCTGTTATG 
GTGGTGACCC TTGATCCAAT GAAAGATCAG TGCTTTGGAG ATGGTTTCAG TCGCTTCCTC 
CTGGACGAGT GCCTGGGTTA TGATGATATT CTAATGTCCA GTGTGAAGGC ATTAGCAGAG 
AATGAGGAAA ACAAAGGCTT CCTCAGGAAC GTGGTGTCTG GAGAACACTA TCGTTTTGTC 
AGCATGTGGA TGGCTCGTAC CTCCTACCTG GCAGCTTTTG TAATAATGGT TATCTTTACT 
CTGTCAGTGT CTATGCTGCT GCGATACTCG CACCACCAAA TTTTTGTTTT TATAGTGGAT 
CTTCTTCAGA TGTTAGAGAT GAATATGACC ATTGCCTTCC CTGCTGCCCC ACTGCTCACT 
GTCATCCTGG CACTTGTGGG GATGGAGGCC ATCATGTCTG AATTTTTCAA TGACACAACC 
ACTGCCTTCT ACATCATCCT CATTGTTTGG TTGGCAGACC AATATGATGC CATCTGTTGC 
CACACCAATA CAAGCAAACG CCACTGGCTC AGATTCTTTT ATCTGTATCA CTTTGCCTTC 
TATGCATATC ATTACCGCTT TAACGGCCAG TACAGCAGTT TGGCTCTTGT CACATCTTGG 
CTCTTCATTC AGCACTCAAT GATCTATTTT TTCCATCACT ACGAGCTGCC GGCTATCTTG 
CAGCAAATCC GGATCCAGGA GATGCTGCTT CAGAACCAGC AGGTTGGTCA AGGAAACCAG 
ACTGCATTGC AGGACAATCT CAACAACAAC ACTAGCACCG CTGCACCAGC TGGCCAAGCT 
AACGCAGCTG CCAACGTGAA CGCTAATGGC CAGGACCAGC AAGCCGCTCA GGACCAACCT 
CTGCTTGAAA CCCACAGCGG CTTGGTGGTG AATCCCAGTC TATCTATCAA TCCATCTGGC 
TCTGATCTCG ACTGGATGGC TGAGATTGCC ATTGGCCCAG ACGTTCTGTC AGTTCCAAAC 
CTAGGTGACC CGCTTTTAGA ACCAGGTGGG ATCCAGGAAG CAACGTCATC CAGCGCAGTT 
GTAACAGAGC TTTGTGGTCC CAACACTGCA GGAGAGAACC AAAATGGGAA CATAAGCACA 
GCCAGCGTTC CTACGGTCAA CGCAAAAGGG AGGAGTTGCG CTCAGGGTCT GGGTCCTCCT 
GGTGGTGGAG GAGGACAGAA AGAATCTGAA ACTAACTCCA GTTCCTCCTC AGTGACCTCA 
CACACAGACT GCAGAGTAGT AGAGTCACGC CGAGGCGCAA CAGACTGGGA CGACAAGGTG 
GAGAATAAAA GTGAAAATTC GGACTCACAC TCCACCCCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156017.1
CDS	799..2883
Translation	MSDNQGNVNNNNVPLNNNNNGGPNRIRNPPMNQNPLINVRDRLFHALFFKMAVTYARLFPPSFRRIFEFLVLLKALFMLFILAYIHIAFSRSPINCLEHVREKWPRDGILRVEIQRNSSRAPIFLQFYETEGIQGLVKEPEEDGHENRQPVVPTDAEEEEEMTMDMFDNSTVRFELDIEPRLNPSMSGGVPGSSLNESQDLSFSQTASKGMQPLRETVSEIEMLTRAVWPQEEYIVEYSLEYGFLRLSQATRQRLNIPVMVVTLDPMKDQCFGDGFSRFLLDECLGYDDILMSSVKALAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMTIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQNQQVGQGNQTALQDNLNNNTSTAAPAGQANAAANVNANGQDQQAAQDQPLLETHSGLVVNPSLSINPSGSDLDWMAEIAIGPDVLSVPNLGDPLLEPGGIQEATSSSAVVTELCGPNTAGENQNGNISTASVPTVNAKGRSCAQGLGPPGGGGGQKESETNSSSSSVTSHTDCRVVESRRGATDWDDKVENKSENSDSHSTPA

Target ORF information:

RefSeq Version	XM_005155960.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 259 (tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155960.3

ORF Insert Sequence:

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGGT ATGCAGCCGC TGAGGGAGAC TGTCTCCGAG 
ATTGAGATGC TAACTCGAGC AGTGTGGCCG CAAGAAGAGT ATATTGTTGA ATATTCTTTG 
GAATATGGTT TCCTAAGGCT ATCCCAGGCC ACACGTCAAC GACTCAACAT CCCTGTTATG 
GTGGTGACCC TTGATCCAAT GAAAGATCAG TGCTTTGGAG ATGGTTTCAG TCGCTTCCTC 
CTGGACGAGT GCCTGGGTTA TGATGATATT CTAATGTCCA GTGTGAAGGC ATTAGCAGAG 
AATGAGGAAA ACAAAGGCTT CCTCAGGAAC GTGGTGTCTG GAGAACACTA TCGTTTTGTC 
AGCATGTGGA TGGCTCGTAC CTCCTACCTG GCAGCTTTTG TAATAATGGT TATCTTTACT 
CTGTCAGTGT CTATGCTGCT GCGATACTCG CACCACCAAA TTTTTGTTTT TATAGTGGAT 
CTTCTTCAGA TGTTAGAGAT GAATATGACC ATTGCCTTCC CTGCTGCCCC ACTGCTCACT 
GTCATCCTGG CACTTGTGGG GATGGAGGCC ATCATGTCTG AATTTTTCAA TGACACAACC 
ACTGCCTTCT ACATCATCCT CATTGTTTGG TTGGCAGACC AATATGATGC CATCTGTTGC 
CACACCAATA CAAGCAAACG CCACTGGCTC AGATTCTTTT ATCTGTATCA CTTTGCCTTC 
TATGCATATC ATTACCGCTT TAACGGCCAG TACAGCAGTT TGGCTCTTGT CACATCTTGG 
CTCTTCATTC AGCACTCAAT GATCTATTTT TTCCATCACT ACGAGCTGCC GGCTATCTTG 
CAGCAAATCC GGATCCAGGA GATGCTGCTT CAGAACCAGC AGGTTGGTCA AGGAAACCAG 
ACTGCATTGC AGGACAATCT CAACAACAAC ACTAGCACCG CTGCACCAGC TGGCCAAGCT 
AACGCAGCTG CCAACGTGAA CGCTAATGGC CAGGACCAGC AAGCCGCTCA GGACCAACCT 
CTGCTTGAAA CCCACAGCGG CTTGGTGGTG AATCCCAGTC TATCTATCAA TCCATCTGGC 
TCTGATCTCG ACTGGATGGC TGAGATTGCC ATTGGCCCAG ACGTTCTGTC AGTTCCAAAC 
CTAGGTGACC CGCTTTTAGA ACCAGGTGGG ATCCAGGAAG CAACGTCATC CAGCGCAGTT 
GTAACAGAGC TTTGTGGTCC CAACACTGCA GGAGAGAACC AAAATGGGAA CATAAGCACA 
GCCAGCGTTC CTACGGTCAA CGCAAAAGGG AGGAGTTGCG CTCAGGGTCT GGGTCCTCCT 
GGTGGTGGAG GAGGACAGAA AGAATCTGAA ACTAACTCCA GTTCCTCCTC AGTGACCTCA 
CACACAGACT GCAGAGTAGT AGAGTCACGC CGAGGCGCAA CAGACTGGGA CGACAAGGTG 
GAGAATAAAA GTGAAAATTC GGACTCACAC TCCACCCCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem259 ( XM_695035.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_700127.3 Danio rerio transmembrane protein 259 (tmem259), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16916
Clone ID Related Accession (Same CDS sequence)	XM_695035.7 , XM_695035.8
Accession Version	XM_695035.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membralin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_695035.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGTG TGGCCGCAAG AAGAGTATAT TGTTGAATAT 
TCTTTGGAAT ATGGTTTCCT AAGGCTATCC CAGGCCACAC GTCAACGACT CAACATCCCT 
GTTATGGTGG TGACCCTTGA TCCAATGAAA GATCAGTGCT TTGGAGATGG TTTCAGTCGC 
TTCCTCCTGG ACGAGTGCCT GGGTTATGAT GATATTCTAA TGTCCAGTGT GAAGGCATTA 
GCAGAGAATG AGGAAAACAA AGGCTTCCTC AGGAACGTGG TGTCTGGAGA ACACTATCGT 
TTTGTCAGCA TGTGGATGGC TCGTACCTCC TACCTGGCAG CTTTTGTAAT AATGGTTATC 
TTTACTCTGT CAGTGTCTAT GCTGCTGCGA TACTCGCACC ACCAAATTTT TGTTTTTATA 
GTGGATCTTC TTCAGATGTT AGAGATGAAT ATGACCATTG CCTTCCCTGC TGCCCCACTG 
CTCACTGTCA TCCTGGCACT TGTGGGGATG GAGGCCATCA TGTCTGAATT TTTCAATGAC 
ACAACCACTG CCTTCTACAT CATCCTCATT GTTTGGTTGG CAGACCAATA TGATGCCATC 
TGTTGCCACA CCAATACAAG CAAACGCCAC TGGCTCAGAT TCTTTTATCT GTATCACTTT 
GCCTTCTATG CATATCATTA CCGCTTTAAC GGCCAGTACA GCAGTTTGGC TCTTGTCACA 
TCTTGGCTCT TCATTCAGCA CTCAATGATC TATTTTTTCC ATCACTACGA GCTGCCGGCT 
ATCTTGCAGC AAATCCGGAT CCAGGAGATG CTGCTTCAGA ACCAGCAGGT TGGTCAAGGA 
AACCAGACTG CATTGCAGGA CAATCTCAAC AACAACACTA GCACCGCTGC ACCAGCTGGC 
CAAGCTAACG CAGCTGCCAA CGTGAACGCT AATGGCCAGG ACCAGCAAGC CGCTCAGGAC 
CAACCTCTGC TTGAAACCCA CAGCGGCTTG GTGGTGAATC CCAGTCTATC TATCAATCCA 
TCTGGCTCTG ATCTCGACTG GATGGCTGAG ATTGCCATTG GCCCAGACGT TCTGTCAGTT 
CCAAACCTAG GTGACCCGCT TTTAGAACCA GGTGGGATCC AGGAAGCAAC GTCATCCAGC 
GCAGTTGTAA CAGAGCTTTG TGGTCCCAAC ACTGCAGGAG AGAACCAAAA TGGGAACATA 
AGCACAGCCA GCGTTCCTAC GGTCAACGCA AAAGGGAGGA GTTGCGCTCA GGGTCTGGGT 
CCTCCTGGTG GTGGAGGAGG ACAGAAAGAA TCTGAAACTA ACTCCAGTTC CTCCTCAGTG 
ACCTCACACA CAGACTGCAG AGTAGTAGAG TCACGCCGAG GCGCAACAGA CTGGGACGAC 
AAGGTGGAGA ATAAAAGTGA AAATTCGGAC TCACACTCCA CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_700127.3
CDS	798..2828
Translation	MSDNQGNVNNNNVPLNNNNNGGPNRIRNPPMNQNPLINVRDRLFHALFFKMAVTYARLFPPSFRRIFEFLVLLKALFMLFILAYIHIAFSRSPINCLEHVREKWPRDGILRVEIQRNSSRAPIFLQFYETEGIQGLVKEPEEDGHENRQPVVPTDAEEEEEMTMDMFDNSTVRFELDIEPRLNPSMSGGVPGSSLNESQDLSFSQTASKVWPQEEYIVEYSLEYGFLRLSQATRQRLNIPVMVVTLDPMKDQCFGDGFSRFLLDECLGYDDILMSSVKALAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMTIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQNQQVGQGNQTALQDNLNNNTSTAAPAGQANAAANVNANGQDQQAAQDQPLLETHSGLVVNPSLSINPSGSDLDWMAEIAIGPDVLSVPNLGDPLLEPGGIQEATSSSAVVTELCGPNTAGENQNGNISTASVPTVNAKGRSCAQGLGPPGGGGGQKESETNSSSSSVTSHTDCRVVESRRGATDWDDKVENKSENSDSHSTPA

Target ORF information:

RefSeq Version	XM_695035.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 259 (tmem259), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_695035.7

ORF Insert Sequence:

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGTG TGGCCGCAAG AAGAGTATAT TGTTGAATAT 
TCTTTGGAAT ATGGTTTCCT AAGGCTATCC CAGGCCACAC GTCAACGACT CAACATCCCT 
GTTATGGTGG TGACCCTTGA TCCAATGAAA GATCAGTGCT TTGGAGATGG TTTCAGTCGC 
TTCCTCCTGG ACGAGTGCCT GGGTTATGAT GATATTCTAA TGTCCAGTGT GAAGGCATTA 
GCAGAGAATG AGGAAAACAA AGGCTTCCTC AGGAACGTGG TGTCTGGAGA ACACTATCGT 
TTTGTCAGCA TGTGGATGGC TCGTACCTCC TACCTGGCAG CTTTTGTAAT AATGGTTATC 
TTTACTCTGT CAGTGTCTAT GCTGCTGCGA TACTCGCACC ACCAAATTTT TGTTTTTATA 
GTGGATCTTC TTCAGATGTT AGAGATGAAT ATGACCATTG CCTTCCCTGC TGCCCCACTG 
CTCACTGTCA TCCTGGCACT TGTGGGGATG GAGGCCATCA TGTCTGAATT TTTCAATGAC 
ACAACCACTG CCTTCTACAT CATCCTCATT GTTTGGTTGG CAGACCAATA TGATGCCATC 
TGTTGCCACA CCAATACAAG CAAACGCCAC TGGCTCAGAT TCTTTTATCT GTATCACTTT 
GCCTTCTATG CATATCATTA CCGCTTTAAC GGCCAGTACA GCAGTTTGGC TCTTGTCACA 
TCTTGGCTCT TCATTCAGCA CTCAATGATC TATTTTTTCC ATCACTACGA GCTGCCGGCT 
ATCTTGCAGC AAATCCGGAT CCAGGAGATG CTGCTTCAGA ACCAGCAGGT TGGTCAAGGA 
AACCAGACTG CATTGCAGGA CAATCTCAAC AACAACACTA GCACCGCTGC ACCAGCTGGC 
CAAGCTAACG CAGCTGCCAA CGTGAACGCT AATGGCCAGG ACCAGCAAGC CGCTCAGGAC 
CAACCTCTGC TTGAAACCCA CAGCGGCTTG GTGGTGAATC CCAGTCTATC TATCAATCCA 
TCTGGCTCTG ATCTCGACTG GATGGCTGAG ATTGCCATTG GCCCAGACGT TCTGTCAGTT 
CCAAACCTAG GTGACCCGCT TTTAGAACCA GGTGGGATCC AGGAAGCAAC GTCATCCAGC 
GCAGTTGTAA CAGAGCTTTG TGGTCCCAAC ACTGCAGGAG AGAACCAAAA TGGGAACATA 
AGCACAGCCA GCGTTCCTAC GGTCAACGCA AAAGGGAGGA GTTGCGCTCA GGGTCTGGGT 
CCTCCTGGTG GTGGAGGAGG ACAGAAAGAA TCTGAAACTA ACTCCAGTTC CTCCTCAGTG 
ACCTCACACA CAGACTGCAG AGTAGTAGAG TCACGCCGAG GCGCAACAGA CTGGGACGAC 
AAGGTGGAGA ATAAAAGTGA AAATTCGGAC TCACACTCCA CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem259 ( XM_695035.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_700127.3 Danio rerio transmembrane protein 259 (tmem259), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16916
Clone ID Related Accession (Same CDS sequence)	XM_695035.7 , XM_695035.8
Accession Version	XM_695035.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membralin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_695035.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGTG TGGCCGCAAG AAGAGTATAT TGTTGAATAT 
TCTTTGGAAT ATGGTTTCCT AAGGCTATCC CAGGCCACAC GTCAACGACT CAACATCCCT 
GTTATGGTGG TGACCCTTGA TCCAATGAAA GATCAGTGCT TTGGAGATGG TTTCAGTCGC 
TTCCTCCTGG ACGAGTGCCT GGGTTATGAT GATATTCTAA TGTCCAGTGT GAAGGCATTA 
GCAGAGAATG AGGAAAACAA AGGCTTCCTC AGGAACGTGG TGTCTGGAGA ACACTATCGT 
TTTGTCAGCA TGTGGATGGC TCGTACCTCC TACCTGGCAG CTTTTGTAAT AATGGTTATC 
TTTACTCTGT CAGTGTCTAT GCTGCTGCGA TACTCGCACC ACCAAATTTT TGTTTTTATA 
GTGGATCTTC TTCAGATGTT AGAGATGAAT ATGACCATTG CCTTCCCTGC TGCCCCACTG 
CTCACTGTCA TCCTGGCACT TGTGGGGATG GAGGCCATCA TGTCTGAATT TTTCAATGAC 
ACAACCACTG CCTTCTACAT CATCCTCATT GTTTGGTTGG CAGACCAATA TGATGCCATC 
TGTTGCCACA CCAATACAAG CAAACGCCAC TGGCTCAGAT TCTTTTATCT GTATCACTTT 
GCCTTCTATG CATATCATTA CCGCTTTAAC GGCCAGTACA GCAGTTTGGC TCTTGTCACA 
TCTTGGCTCT TCATTCAGCA CTCAATGATC TATTTTTTCC ATCACTACGA GCTGCCGGCT 
ATCTTGCAGC AAATCCGGAT CCAGGAGATG CTGCTTCAGA ACCAGCAGGT TGGTCAAGGA 
AACCAGACTG CATTGCAGGA CAATCTCAAC AACAACACTA GCACCGCTGC ACCAGCTGGC 
CAAGCTAACG CAGCTGCCAA CGTGAACGCT AATGGCCAGG ACCAGCAAGC CGCTCAGGAC 
CAACCTCTGC TTGAAACCCA CAGCGGCTTG GTGGTGAATC CCAGTCTATC TATCAATCCA 
TCTGGCTCTG ATCTCGACTG GATGGCTGAG ATTGCCATTG GCCCAGACGT TCTGTCAGTT 
CCAAACCTAG GTGACCCGCT TTTAGAACCA GGTGGGATCC AGGAAGCAAC GTCATCCAGC 
GCAGTTGTAA CAGAGCTTTG TGGTCCCAAC ACTGCAGGAG AGAACCAAAA TGGGAACATA 
AGCACAGCCA GCGTTCCTAC GGTCAACGCA AAAGGGAGGA GTTGCGCTCA GGGTCTGGGT 
CCTCCTGGTG GTGGAGGAGG ACAGAAAGAA TCTGAAACTA ACTCCAGTTC CTCCTCAGTG 
ACCTCACACA CAGACTGCAG AGTAGTAGAG TCACGCCGAG GCGCAACAGA CTGGGACGAC 
AAGGTGGAGA ATAAAAGTGA AAATTCGGAC TCACACTCCA CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_700127.3
CDS	799..2829
Translation	MSDNQGNVNNNNVPLNNNNNGGPNRIRNPPMNQNPLINVRDRLFHALFFKMAVTYARLFPPSFRRIFEFLVLLKALFMLFILAYIHIAFSRSPINCLEHVREKWPRDGILRVEIQRNSSRAPIFLQFYETEGIQGLVKEPEEDGHENRQPVVPTDAEEEEEMTMDMFDNSTVRFELDIEPRLNPSMSGGVPGSSLNESQDLSFSQTASKVWPQEEYIVEYSLEYGFLRLSQATRQRLNIPVMVVTLDPMKDQCFGDGFSRFLLDECLGYDDILMSSVKALAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMTIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQNQQVGQGNQTALQDNLNNNTSTAAPAGQANAAANVNANGQDQQAAQDQPLLETHSGLVVNPSLSINPSGSDLDWMAEIAIGPDVLSVPNLGDPLLEPGGIQEATSSSAVVTELCGPNTAGENQNGNISTASVPTVNAKGRSCAQGLGPPGGGGGQKESETNSSSSSVTSHTDCRVVESRRGATDWDDKVENKSENSDSHSTPA

Target ORF information:

RefSeq Version	XM_695035.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 259 (tmem259), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_695035.8

ORF Insert Sequence:

ATGTCTGACA ACCAAGGCAA TGTGAACAAC AACAACGTTC CACTGAACAA CAATAATAAT 
GGTGGGCCAA ACCGGATTCG TAACCCCCCA ATGAACCAGA ACCCCTTGAT TAACGTGAGA 
GACAGACTCT TCCACGCATT ATTCTTTAAG ATGGCAGTCA CATACGCCAG ACTATTCCCA 
CCATCTTTCA GACGAATATT TGAGTTCCTG GTCTTGTTAA AGGCACTGTT TATGCTCTTC 
ATCCTGGCCT ACATCCACAT TGCCTTCTCG CGCTCTCCAA TAAACTGCCT GGAGCATGTG 
CGGGAGAAAT GGCCCCGCGA CGGCATCCTG CGTGTGGAGA TCCAGCGCAA CTCCAGCCGT 
GCTCCTATCT TTCTGCAATT TTACGAGACT GAGGGTATCC AGGGACTCGT GAAGGAACCA 
GAGGAGGATG GACATGAAAA CAGACAGCCA GTGGTGCCAA CAGATGCTGA AGAGGAGGAA 
GAGATGACTA TGGACATGTT TGACAACAGC ACAGTACGAT TTGAGCTGGA CATCGAGCCG 
CGTCTGAATC CATCGATGAG CGGTGGTGTT CCTGGCTCCA GCCTGAACGA ATCCCAGGAT 
CTTTCCTTCA GCCAGACCGC CTCTAAAGTG TGGCCGCAAG AAGAGTATAT TGTTGAATAT 
TCTTTGGAAT ATGGTTTCCT AAGGCTATCC CAGGCCACAC GTCAACGACT CAACATCCCT 
GTTATGGTGG TGACCCTTGA TCCAATGAAA GATCAGTGCT TTGGAGATGG TTTCAGTCGC 
TTCCTCCTGG ACGAGTGCCT GGGTTATGAT GATATTCTAA TGTCCAGTGT GAAGGCATTA 
GCAGAGAATG AGGAAAACAA AGGCTTCCTC AGGAACGTGG TGTCTGGAGA ACACTATCGT 
TTTGTCAGCA TGTGGATGGC TCGTACCTCC TACCTGGCAG CTTTTGTAAT AATGGTTATC 
TTTACTCTGT CAGTGTCTAT GCTGCTGCGA TACTCGCACC ACCAAATTTT TGTTTTTATA 
GTGGATCTTC TTCAGATGTT AGAGATGAAT ATGACCATTG CCTTCCCTGC TGCCCCACTG 
CTCACTGTCA TCCTGGCACT TGTGGGGATG GAGGCCATCA TGTCTGAATT TTTCAATGAC 
ACAACCACTG CCTTCTACAT CATCCTCATT GTTTGGTTGG CAGACCAATA TGATGCCATC 
TGTTGCCACA CCAATACAAG CAAACGCCAC TGGCTCAGAT TCTTTTATCT GTATCACTTT 
GCCTTCTATG CATATCATTA CCGCTTTAAC GGCCAGTACA GCAGTTTGGC TCTTGTCACA 
TCTTGGCTCT TCATTCAGCA CTCAATGATC TATTTTTTCC ATCACTACGA GCTGCCGGCT 
ATCTTGCAGC AAATCCGGAT CCAGGAGATG CTGCTTCAGA ACCAGCAGGT TGGTCAAGGA 
AACCAGACTG CATTGCAGGA CAATCTCAAC AACAACACTA GCACCGCTGC ACCAGCTGGC 
CAAGCTAACG CAGCTGCCAA CGTGAACGCT AATGGCCAGG ACCAGCAAGC CGCTCAGGAC 
CAACCTCTGC TTGAAACCCA CAGCGGCTTG GTGGTGAATC CCAGTCTATC TATCAATCCA 
TCTGGCTCTG ATCTCGACTG GATGGCTGAG ATTGCCATTG GCCCAGACGT TCTGTCAGTT 
CCAAACCTAG GTGACCCGCT TTTAGAACCA GGTGGGATCC AGGAAGCAAC GTCATCCAGC 
GCAGTTGTAA CAGAGCTTTG TGGTCCCAAC ACTGCAGGAG AGAACCAAAA TGGGAACATA 
AGCACAGCCA GCGTTCCTAC GGTCAACGCA AAAGGGAGGA GTTGCGCTCA GGGTCTGGGT 
CCTCCTGGTG GTGGAGGAGG ACAGAAAGAA TCTGAAACTA ACTCCAGTTC CTCCTCAGTG 
ACCTCACACA CAGACTGCAG AGTAGTAGAG TCACGCCGAG GCGCAACAGA CTGGGACGAC 
AAGGTGGAGA ATAAAAGTGA AAATTCGGAC TCACACTCCA CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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tmem259 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム