slc12a10.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a10.2
Entrez Gene ID	562834
Full Name	solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 2
Synonyms	ncc2b,si:dkey-114c3.2
General protein information	Preferred Names solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 2 Names solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 2 solute carrier family 12 (sodium/potassium/chloride transporters), member 10.2 solute carrier family 12 member 10.2 zNCC-like 2 znccg
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045001.1	NP_001038466.1	solute carrier family 12 member 10, tandem duplicate 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100003286	NP_001154832.1
Danio rerio (zebrafish)	slc12a10.3	NP_001128603.1
Danio rerio (zebrafish)	slc12a10.2	NP_001038466.1
Danio rerio (zebrafish)	slc12a10.1	NP_001154850.1
Xenopus tropicalis (tropical clawed frog)	slc12a3.2	XP_002934326.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-Wide Meta-Analysis Unravels Interactions between Magnesium Homeostasis and Metabolic Phenotypes.
Corre T, Arjona FJ, Hayward C, Youhanna S, de Baaij JHF, Belge H, N?gele N, Debaix H, Blanchard MG, Traglia M, Harris SE, Ulivi S, Rueedi R, Lamparter D, Mac? A, Sala C, Lenarduzzi S, Ponte B, Pruijm M, Ackermann D, Ehret G, Baptista D, Polasek O, Rudan I, Hurd TW, Hastie ND, Vitart V, Waeber G, Kutalik Z, Bergmann S, Vargas-Poussou R, Konrad M, Gasparini P, Deary IJ, Starr JM, Toniolo D, Vollenweider P, Hoenderop JGJ, Bindels RJM, Bochud M, Devuyst O
Journal of the American Society of Nephrology : JASN29(1)335-348(2018 Jan)

A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.
Kwong RW, Perry SF
American journal of physiology. Cell physiology311(6)C931-C941(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fish-specific NCC2b and CLC-2c may have coevolved to perform extra-renal Cl(-) uptake during the evolution of vertebrates in an aquatic environment.
Title: Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.

Prolactin (PRL) signaling through PRL receptors in the gill regulates the expression of ncc.
Title: Prolactin regulates transcription of the ion uptake Na+/Cl- cotransporter (ncc) gene in zebrafish gill.

There is a possible role of the Na(+)-Cl(-) cotransporter, SLC12A10.2, in the Cl(-) uptake mechanism in zebrafish skin/gills.
Title: Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio).

The following slc12a10.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a10.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01546	NM_001045001.1 Latest version!	Danio rerio solute carrier family 12 member 10, tandem duplicate 2 (slc12a10.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a10.2 ( NM_001045001.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038466.1 Danio rerio solute carrier family 12 member 10, tandem duplicate 2 (slc12a10.2), mRNA.

Price & Availability↑

CloneID	ODa01546
Clone ID Related Accession (Same CDS sequence)	NM_001045001.1
Accession Version	NM_001045001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-22
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 10, tandem duplicate 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR383683.17. On Sep 1, 2006 this sequence version replaced XM_686209.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01004594.1, GFIL01038211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAAT ATATTGAACG CATCAGAAAT GTAATCCCGG GAATGCGCCG CAGGGACAGC 
AATGTCCCCC ATCAAGGGGA CGTGGCTGTG GATATGGGAG AGGCTGATTT TGGCAAGAAG 
GAAAATCAAA AAATGTCAAT CTACAATCCC CTCGACCCCG TGCCGAAATA TGACTTTTAC 
GCCAAAGACA CGTGGGCAGG ACGTATTAAA AAGAGCAGAC CTTCACTGGA TGTTCTGCGC 
AACCCACCGG ATGACATTAC ACCAACACCA GATGAAGAAG ATGGACGGCC CAAAGTCAAG 
CTGGTCCGCT TTGGGTGGGT TCTAGGAGTT TGGATTCGCT GCATGTTGAA CATCTGGGGA 
GTCATCATGT ACCTGCGGCT GCCGTGGGTC ACATCTCAAG CCGGACTTGT TCTGACATTC 
GTCATCATCT TCATGTCTGT CACCATCACG ACGATCACGG CGACATCAGT GTCTGCAATC 
TCCACCAATG GGAAAGTCTA CTCTGGTGGT ACATACTTCA TGATCTCTCG GAGTCTGGGT 
CCAGAGCTTG GAGCGCCCAT CGGTTTGCTC TTTGCGTTTG CCTGTTCTAT CGCCTGTGCT 
CTTCACACAG TAGGCTTTTC AGAAACGGTC AGAGATATGC TTAAAGACTT TAAATCTCAG 
ATGGTGGACG ATGTGAATGA CGTGCGGATC ATTGGGGCGA TTACTGTCAC CATCTGCCTC 
CTCATCACAT TCGCTGGCAT GGCATGGGAG GCGAAGGCTC AGATCTTGTT CTTCATCGCC 
ATCATACTTT CATTGCTAAA TTATTTTGTG GGCACGGTCA TTCCCCCCAC CCAAGAGAAG 
GAGGCAGTGG GATTTTTTGG CTACCACTCT GATATTTTCG TATCAAATCT GCTGCCTTCC 
TTCCGAGGTC CAAGTGGAAG TTTCTTAGTG GTTTTTGCTA TCTTCTTCCC TGCCTGCACA 
GGAATTCTGT CTGGTGTCAA CATCTGTGGA GACCTCAAGG ATCCAACAGG TGGGATTCCT 
AAAGGGACCC TACTGTCAAT TTTATGCACC ACTCTGAGCT ATTTGCTGAT CGCTGCCACG 
TGTGCTGCCA CTGTGGTAAG AGATGCGTCT GGGAATAAAA ATGATAGTTT AGCCATATCA 
AATTACACAA TCCACTGCAG CGGTCTGGGC TGTAAATATG GCTGGAATTT TGACAGCTGT 
GAACAATACC GAAACTGTAC ACAAGGACTG GCAAACAACC TCCGGATTCT TACCGTTGTG 
TCAGGATGGG GCATTCTGAT CACTATTGGG ACCTTTGCTG CAACCTTGTC ATCAGCGCTG 
GGCTTCCTGG TGTCCGCCCC CAAAGTTTTC CAGTTATTGT GTAAGGACAA GATTTACCCA 
TACATTGGCT TCTTCGGAAA AGGTTATGGG AAAAACAAGG AGCCTCTTCG TGCTTACGTT 
CTCACGTTTA TCATTGCCAT GTGTTTCATC CTCATCGGTA ATTTGAACAC AATCGCTCCT 
CTGATCTCCA ACTTCTTCCT CGCTTCTTAC GGCCTTATTA ACTTCAGCTG TTTCCATGCC 
TCTATCACCA AATCACCTGG TTGGCGACCA CAGTACCGGT ACTATTCTCC ATGGCTGTCT 
CTGTTCTGCT GTTGCTTGTC GTTCACCCTC ATGTTTTTGT TCACATGGTG GGCTGCTCTT 
GTCACTTTCA GTGTTGTTCT CTTCCTCATA GGTTATGTCA ACTATAAAAA AACCTATGTC 
AACTGGGGCT CGTCCTACCA AGCCGCGTTC TACAACATGG CTTTGAACTT CTCGATGAAT 
CTGACTACTG TGCCGGATCA CGTCAAAAAC TTTAGGCCTC AGTGTCTTGT TCTGTCTGGA 
CCTCCAAACA CCCGTCCTGC TCTGGTGGAC TTTGTGGGCA CTTTTACCAA AAATGTCAGC 
CTGATGATCT GTGGCAACAT AACCATAGAG GGCGAGCAGT CCGATTTTCC TGAACACAGT 
TCTGATTTGG TGGTGGAATG GCTGACTAAG AGGAAAGTTC GTTCTTTCTA CACAACTTTC 
GCTGCTGAAA GTCTGCAGGA TGGCGCCCAC AATCTCATGC TGGCTTCTGG TCTTGGGAAA 
CTGAAGCCAA ACACACTTGT TTTGGGATAC AAGACAAACT GGCAGGAATG TAAACCGGAG 
AGTTTGCAGG ACTACATCAA CACCATTAAT GATGCGTTTG ATTCAAACTA CGGTGTCGCA 
CTTTTGAGAA TGATGGATAA GCTGGACATT ACAGATGATA TGTACAGTGC AGAAGACGGC 
TTATCTGCTA TTGATAATCC TGCCTTTGAG AGAGATGAAG TCCCACAATC CAGAAGTGAC 
ACCATTGATC GGGATTCAGA TAATTCTGAT GAAGAATCAA GCGATCAAAT CAGAACAGTG 
TTTCAGGCCA AACAAGGCAA GAAGACCATT GACATCTACT GGATCTCTGA TGATGGAGGT 
CTGACTCTTC TAGTGCCGTA TTTGCTGACC AGAAGGAACC GCTGGAAAAA GAGCAAAATC 
CGAGTGTTTA TCTTAGGAGA TCAAGAGACC AAGAAAGAAG ACCGCGACGA CATGAAGATG 
CTGCTAAAGA GATTCCGGCT GGAAATTGAG GAAGTTATTG TCATAACAGA TGTGGATAAA 
CCTCCCCTAG CTAAAAATGT GCAGCGATTT GAGGACACCA TCACCCCCTT TAGGCTGAGT 
GAAGAACAGA CCAGAGTAGA CCTGCAGGAG TTCAGGAGAC TCAATCCGTG GGCAGTTTCT 
GACAAAGATC TGGAGGCTGT TAGACCCAAG GTGGAGAGGA CGGTGCGACT AAATGAGATC 
ATCAAGAAGA ACTCCCTCTA CGCTGCTCTG GTTGTAGTTT CTCTCCCAGT GCCGGATCTC 
AACTGTCCCA GCTCCCTCTA TATGGCCTGG ATGGACGCGC TGAGTATCGG TATCAACTGT 
CCCGCCCTGC TCATCCGAGG AAACCAGGAG AACGTCATGA CCTTCTACTG CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038466.1
CDS	1..2997
Translation	MAKYIERIRNVIPGMRRRDSNVPHQGDVAVDMGEADFGKKENQKMSIYNPLDPVPKYDFYAKDTWAGRIKKSRPSLDVLRNPPDDITPTPDEEDGRPKVKLVRFGWVLGVWIRCMLNIWGVIMYLRLPWVTSQAGLVLTFVIIFMSVTITTITATSVSAISTNGKVYSGGTYFMISRSLGPELGAPIGLLFAFACSIACALHTVGFSETVRDMLKDFKSQMVDDVNDVRIIGAITVTICLLITFAGMAWEAKAQILFFIAIILSLLNYFVGTVIPPTQEKEAVGFFGYHSDIFVSNLLPSFRGPSGSFLVVFAIFFPACTGILSGVNICGDLKDPTGGIPKGTLLSILCTTLSYLLIAATCAATVVRDASGNKNDSLAISNYTIHCSGLGCKYGWNFDSCEQYRNCTQGLANNLRILTVVSGWGILITIGTFAATLSSALGFLVSAPKVFQLLCKDKIYPYIGFFGKGYGKNKEPLRAYVLTFIIAMCFILIGNLNTIAPLISNFFLASYGLINFSCFHASITKSPGWRPQYRYYSPWLSLFCCCLSFTLMFLFTWWAALVTFSVVLFLIGYVNYKKTYVNWGSSYQAAFYNMALNFSMNLTTVPDHVKNFRPQCLVLSGPPNTRPALVDFVGTFTKNVSLMICGNITIEGEQSDFPEHSSDLVVEWLTKRKVRSFYTTFAAESLQDGAHNLMLASGLGKLKPNTLVLGYKTNWQECKPESLQDYINTINDAFDSNYGVALLRMMDKLDITDDMYSAEDGLSAIDNPAFERDEVPQSRSDTIDRDSDNSDEESSDQIRTVFQAKQGKKTIDIYWISDDGGLTLLVPYLLTRRNRWKKSKIRVFILGDQETKKEDRDDMKMLLKRFRLEIEEVIVITDVDKPPLAKNVQRFEDTITPFRLSEEQTRVDLQEFRRLNPWAVSDKDLEAVRPKVERTVRLNEIIKKNSLYAALVVVSLPVPDLNCPSSLYMAWMDALSIGINCPALLIRGNQENVMTFYCQ

Target ORF information:

RefSeq Version	NM_001045001.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 10, tandem duplicate 2 (slc12a10.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045001.1

ORF Insert Sequence:

ATGGCCAAAT ATATTGAACG CATCAGAAAT GTAATCCCGG GAATGCGCCG CAGGGACAGC 
AATGTCCCCC ATCAAGGGGA CGTGGCTGTG GATATGGGAG AGGCTGATTT TGGCAAGAAG 
GAAAATCAAA AAATGTCAAT CTACAATCCC CTCGACCCCG TGCCGAAATA TGACTTTTAC 
GCCAAAGACA CGTGGGCAGG ACGTATTAAA AAGAGCAGAC CTTCACTGGA TGTTCTGCGC 
AACCCACCGG ATGACATTAC ACCAACACCA GATGAAGAAG ATGGACGGCC CAAAGTCAAG 
CTGGTCCGCT TTGGGTGGGT TCTAGGAGTT TGGATTCGCT GCATGTTGAA CATCTGGGGA 
GTCATCATGT ACCTGCGGCT GCCGTGGGTC ACATCTCAAG CCGGACTTGT TCTGACATTC 
GTCATCATCT TCATGTCTGT CACCATCACG ACGATCACGG CGACATCAGT GTCTGCAATC 
TCCACCAATG GGAAAGTCTA CTCTGGTGGT ACATACTTCA TGATCTCTCG GAGTCTGGGT 
CCAGAGCTTG GAGCGCCCAT CGGTTTGCTC TTTGCGTTTG CCTGTTCTAT CGCCTGTGCT 
CTTCACACAG TAGGCTTTTC AGAAACGGTC AGAGATATGC TTAAAGACTT TAAATCTCAG 
ATGGTGGACG ATGTGAATGA CGTGCGGATC ATTGGGGCGA TTACTGTCAC CATCTGCCTC 
CTCATCACAT TCGCTGGCAT GGCATGGGAG GCGAAGGCTC AGATCTTGTT CTTCATCGCC 
ATCATACTTT CATTGCTAAA TTATTTTGTG GGCACGGTCA TTCCCCCCAC CCAAGAGAAG 
GAGGCAGTGG GATTTTTTGG CTACCACTCT GATATTTTCG TATCAAATCT GCTGCCTTCC 
TTCCGAGGTC CAAGTGGAAG TTTCTTAGTG GTTTTTGCTA TCTTCTTCCC TGCCTGCACA 
GGAATTCTGT CTGGTGTCAA CATCTGTGGA GACCTCAAGG ATCCAACAGG TGGGATTCCT 
AAAGGGACCC TACTGTCAAT TTTATGCACC ACTCTGAGCT ATTTGCTGAT CGCTGCCACG 
TGTGCTGCCA CTGTGGTAAG AGATGCGTCT GGGAATAAAA ATGATAGTTT AGCCATATCA 
AATTACACAA TCCACTGCAG CGGTCTGGGC TGTAAATATG GCTGGAATTT TGACAGCTGT 
GAACAATACC GAAACTGTAC ACAAGGACTG GCAAACAACC TCCGGATTCT TACCGTTGTG 
TCAGGATGGG GCATTCTGAT CACTATTGGG ACCTTTGCTG CAACCTTGTC ATCAGCGCTG 
GGCTTCCTGG TGTCCGCCCC CAAAGTTTTC CAGTTATTGT GTAAGGACAA GATTTACCCA 
TACATTGGCT TCTTCGGAAA AGGTTATGGG AAAAACAAGG AGCCTCTTCG TGCTTACGTT 
CTCACGTTTA TCATTGCCAT GTGTTTCATC CTCATCGGTA ATTTGAACAC AATCGCTCCT 
CTGATCTCCA ACTTCTTCCT CGCTTCTTAC GGCCTTATTA ACTTCAGCTG TTTCCATGCC 
TCTATCACCA AATCACCTGG TTGGCGACCA CAGTACCGGT ACTATTCTCC ATGGCTGTCT 
CTGTTCTGCT GTTGCTTGTC GTTCACCCTC ATGTTTTTGT TCACATGGTG GGCTGCTCTT 
GTCACTTTCA GTGTTGTTCT CTTCCTCATA GGTTATGTCA ACTATAAAAA AACCTATGTC 
AACTGGGGCT CGTCCTACCA AGCCGCGTTC TACAACATGG CTTTGAACTT CTCGATGAAT 
CTGACTACTG TGCCGGATCA CGTCAAAAAC TTTAGGCCTC AGTGTCTTGT TCTGTCTGGA 
CCTCCAAACA CCCGTCCTGC TCTGGTGGAC TTTGTGGGCA CTTTTACCAA AAATGTCAGC 
CTGATGATCT GTGGCAACAT AACCATAGAG GGCGAGCAGT CCGATTTTCC TGAACACAGT 
TCTGATTTGG TGGTGGAATG GCTGACTAAG AGGAAAGTTC GTTCTTTCTA CACAACTTTC 
GCTGCTGAAA GTCTGCAGGA TGGCGCCCAC AATCTCATGC TGGCTTCTGG TCTTGGGAAA 
CTGAAGCCAA ACACACTTGT TTTGGGATAC AAGACAAACT GGCAGGAATG TAAACCGGAG 
AGTTTGCAGG ACTACATCAA CACCATTAAT GATGCGTTTG ATTCAAACTA CGGTGTCGCA 
CTTTTGAGAA TGATGGATAA GCTGGACATT ACAGATGATA TGTACAGTGC AGAAGACGGC 
TTATCTGCTA TTGATAATCC TGCCTTTGAG AGAGATGAAG TCCCACAATC CAGAAGTGAC 
ACCATTGATC GGGATTCAGA TAATTCTGAT GAAGAATCAA GCGATCAAAT CAGAACAGTG 
TTTCAGGCCA AACAAGGCAA GAAGACCATT GACATCTACT GGATCTCTGA TGATGGAGGT 
CTGACTCTTC TAGTGCCGTA TTTGCTGACC AGAAGGAACC GCTGGAAAAA GAGCAAAATC 
CGAGTGTTTA TCTTAGGAGA TCAAGAGACC AAGAAAGAAG ACCGCGACGA CATGAAGATG 
CTGCTAAAGA GATTCCGGCT GGAAATTGAG GAAGTTATTG TCATAACAGA TGTGGATAAA 
CCTCCCCTAG CTAAAAATGT GCAGCGATTT GAGGACACCA TCACCCCCTT TAGGCTGAGT 
GAAGAACAGA CCAGAGTAGA CCTGCAGGAG TTCAGGAGAC TCAATCCGTG GGCAGTTTCT 
GACAAAGATC TGGAGGCTGT TAGACCCAAG GTGGAGAGGA CGGTGCGACT AAATGAGATC 
ATCAAGAAGA ACTCCCTCTA CGCTGCTCTG GTTGTAGTTT CTCTCCCAGT GCCGGATCTC 
AACTGTCCCA GCTCCCTCTA TATGGCCTGG ATGGACGCGC TGAGTATCGG TATCAACTGT 
CCCGCCCTGC TCATCCGAGG AAACCAGGAG AACGTCATGA CCTTCTACTG CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-Wide Meta-Analysis Unravels Interactions between Magnesium Homeostasis and Metabolic Phenotypes.
Journal of the American Society of Nephrology : JASN29(1)335-348(2018 Jan)
Corre T,Arjona FJ,Hayward C,Youhanna S,de Baaij JHF,Belge H,N?gele N,Debaix H,Blanchard MG,Traglia M,Harris SE,Ulivi S,Rueedi R,Lamparter D,Mac? A,Sala C,Lenarduzzi S,Ponte B,Pruijm M,Ackermann D,Ehret G,Baptista D,Polasek O,Rudan I,Hurd TW,Hastie ND,Vitart V,Waeber G,Kutalik Z,Bergmann S,Vargas-Poussou R,Konrad M,Gasparini P,Deary IJ,Starr JM,Toniolo D,Vollenweider P,Hoenderop JGJ,Bindels RJM,Bochud M,Devuyst O

A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.
American journal of physiology. Cell physiology311(6)C931-C941(2016 Dec)
Kwong RW,Perry SF