slc12a10.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a10.3
Entrez Gene ID	562763
Full Name	slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3
Synonyms	si:dkey-114c3.3
General protein information	Preferred Names slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 Names slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10.3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021477565.1	XP_021333240.1	slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 isoform X1
NM_001135131.1	NP_001128603.1	solute carrier family 12 member 10, tandem duplicate 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100003286	NP_001154832.1
Danio rerio (zebrafish)	slc12a10.3	NP_001128603.1
Danio rerio (zebrafish)	slc12a10.2	NP_001038466.1
Danio rerio (zebrafish)	slc12a10.1	NP_001154850.1
Xenopus tropicalis (tropical clawed frog)	slc12a3.2	XP_002934326.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.
Wang YF, Yan JJ, Tseng YC, Chen RD, Hwang PP
International journal of biological sciences11(10)1190-203(2015)

A new model for fish ion regulation: identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes).
Hsu HH, Lin LY, Tseng YC, Horng JL, Hwang PP
Cell and tissue research357(1)225-43(2014 Jul)

Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio).
Wang YF, Tseng YC, Yan JJ, Hiroi J, Hwang PP
American journal of physiology. Regulatory, integrative and comparative physiology296(5)R1650-60(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slc12a10.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a10.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35620	NM_001135131.1 Latest version!	Danio rerio solute carrier family 12 member 10, tandem duplicate 3 (slc12a10.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa12476	XM_021477565.1 Latest version!	Danio rerio slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 (slc12a10.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a10.3 ( NM_001135131.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001128603.1 Danio rerio solute carrier family 12 member 10, tandem duplicate 3 (slc12a10.3), mRNA.

Price & Availability↑

CloneID	ODa35620
Clone ID Related Accession (Same CDS sequence)	NM_001135131.1
Accession Version	NM_001135131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 10, tandem duplicate 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168511.1. On or before Aug 31, 2013 this sequence version replaced XM_005161757.1, XM_686141.3. ##Evidence-Data-START## CDS exon combination :: BC168511.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAATTTGG AAAAGGTTGA TTATGTGATG GAGCAGGAGC AGGACAAGAG AAAAATGTCA 
ATCTTCAATC CCCTTGATCC CATACCTAAA TTTGAATTTT ATGCTAAAGA CACCTGGACT 
GGACGTATAA AAAGTGCGAG ACCTTCACTA GATGTTCTGC GCAAATCACC TGACCCTGAT 
GATCTTCCTC CACCACCGGA GGATGATGGA CGGCCCAAAG TCAAACTCGT TCGCTTTGGA 
TGGGTTCTGG GAGTCATGAT TCGCTGTATG CTGAATATCT GGGGAGTCAT CATGTTTCTG 
CGGCTGCCGT GGATCACGTC TCAAGCTGGA ATCATTCTGA CATACGTCAT CATCTTCATG 
TCTGTCACCA TCACAACAAT CACAGCGACG TCAGTGTCTG CAATCTCCAC CAATGGGAAG 
GTCTACTCTG GTGGTACGTA CTTCTTGATT TCTCGAAGTC TGGGTCCAGA GCTCGGAGCG 
CCCATTGGTT TGCTCTTCTC GTTCGCCAAT GCTCTCGCCT GCTCGCTCAA CACTGTGGGG 
TTTGCAGAAA CCGTCAGAGA CGTTCTCAAA ATGGTGGACG ATGTGAATGA CGTGCGGATT 
ATTGGGACGA TTACAGTCTG CATCCTCCTC CTCATAACGT TTGCAGGCAT GGCATGGGAG 
GCGAAGGCTC AGATCCTGTT CTTCATTGCG CTGTTGATCT CCTTAGCTAA TTATTTTGTT 
GGGACGCTCC TTCCTCCCAC CCCAGAGAAG CAGGCGGTGG GATTTTTCGG CTACCGCGCT 
GAGATCTTCG TGGATAATCT GCTGCCATCA TTCAGAGGAA AAGACGGCTC TTTCTTCAGG 
ATGTTTTCCA TCTTCTTTCC ATCTGCCACC GGAATTCTGT CTGGCGTCAA CATTTGTGGA 
GATCTCAAGG ATCCCTCCGG TGGGATTCCT AAAGGGACCC TGCTGGCCAT TTTCTGGACC 
ACAGTCAGCT ATTTACTCAT TGCAATTACC GTCGCTGCAA GTGTGGTAAG AGATGCTTCT 
GGCAATCTTA ATGACAGTCT AGCGTTCGAT TCCTCAATCC AGTGCAGTGG CTTGGGCTGT 
AAACTGGGCT GGAATTTTGA CCACTGTGAA CAAAACCACA CCTGTAGCTT TGGACTGGCA 
AACTACTTCC AGATTCTCAC CACTGCCTCT GGAACTGGTC ATCTGATCAC CATCGGGATT 
TTTGCAGCTA CTTTGTCGTC AGCATTGGGT TTCTTGGTTT CTGCACCCAA AATATTTCAG 
TGTCTGTGCA AAGATAACAT TTACCCCTAC ATCGGCTTCT TCGGGAAAGG CTACGGGAAA 
AACCAAGAAC CTCTACGTGG ATATTTGCTG ATGTTCTTCA TTGCGTTGGC CTTCATCCTC 
ATCGGTGACC TGAACACAAT CGCTCCTCTG ATCTCCAACT TCTTCCTCTG CTCTTACGGC 
CTCATCAACT TCAGCTGCTT CCACGCCACG ATCACCAAAT CACCTGGTTG GCGTCCACAT 
TACCACTACT TTTCTCCATG GACGTCTCTG TTTGCTGCTT TCCTCTCATT CATCCTCATG 
TTTTTGTTTA CGTGGTGGGC TGCTCTGGTC ACGTTTGCTA TCGTCCTCTC CCTCTTAGGA 
TACGTCACCT ACAAGAAGCC TAAGATTAAC TGGGGAACGT CCTATCAGGC CAGTTTCTAC 
AACATGGCTC TGTCCTTCTC AATGTCCCTG ACTGGTGTTG AAGACCATGT TAAAAACTTC 
AGGCCTCAGT GTCTGGTTCT GACTGGCCCT CCAAACGTCC GTCCAGCCCT GGTGGACTTT 
GTGGGCACTT TCACTAAAAA CATCAGCCTG ATGATCTGTG GGAACATTTT CATGGTGGAG 
GAGAAGTCCA GGTTTCCACA GCACAGCACT GATATGCTGG TGGACTGGCT GAATCAGAGG 
AAAGTTCGTG CTTTTTACAC GTCCTTCACT GCGGACAGTC TGACAGAGGG AACACACAGT 
CTCATGCAGG CTTCAGGTCT TGGGAAACTG AAGCCAAACA CTCTTGTTTT GGGATATAAG 
ACAAACTGGC AGGAATGTAA ACCAGAGAGA TTACAGGACT ACTTCAACAC CATCAGCGAT 
GCGTTTGACT CCAGCTATGG TATTGCGGTT CTGAGAATGA TGGATGGATT GGACATAAGA 
GAAGATTTGA CTTCAGGCCG ATCGTCTGCA ATTGACAATC CAGCATTTGA GGCAGATGAA 
AATACAAAAT CAGAAAATGA AGAGACCGAC CGAAATTCAG ATATTTCTGA CGATGGCTCC 
AACGATCAGG TCAAAACAGT GTTTCAGACC AAACAGGGCA GGAAGACCAT CGACATCTAC 
TGGATCTCTG ATGATGGAGG TCTGACTCTT CTAGTGCCAT ATTTGCTGAC ACGACGAAAC 
CGCTGGAAGA AGAGCAAAAT CAGAGTGTTT GTCTTAGGAG ATCAAGAGAC CATGAAGGAG 
GACCATAAAG ACATGAAGAT GCTGTTAAAG AGATTCCGGC TAGAGATCGA GGACATCATC 
GTGATCACAG ATGTGGATAA ACCTCCGCTA GCTAAAAATT TGCAGAGGTA TGATGACTCC 
GTCGCCCCCT TCAGGCTGAC TGAGGAACAG AGCAGAGGAG ACCTGCAGGA GTTCAGGAGA 
CTCAATCCGT GGACGGTTTC TGACAAAGAC CTGGAGGCTA TTAGACCCAA GATTGAGAGG 
ACCGTGCGAC TAAATGAAAT CATCAAGAAG AACTCCAACC ACGCTGCTTT GGTTGTAGTT 
TCTCTCCCAG TGCCGGATCT CAACTGTCCC AGTTCTCTCT ATATGGCCTG GATGGAGGCG 
CTGAGTTTTG GGATACACTG TCCTGCCCTG CTCATCCGAG GAAACCAGGA GAACGTCATG 
ACCTTCTACT GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128603.1
CDS	203..3100
Translation	MNLEKVDYVMEQEQDKRKMSIFNPLDPIPKFEFYAKDTWTGRIKSARPSLDVLRKSPDPDDLPPPPEDDGRPKVKLVRFGWVLGVMIRCMLNIWGVIMFLRLPWITSQAGIILTYVIIFMSVTITTITATSVSAISTNGKVYSGGTYFLISRSLGPELGAPIGLLFSFANALACSLNTVGFAETVRDVLKMVDDVNDVRIIGTITVCILLLITFAGMAWEAKAQILFFIALLISLANYFVGTLLPPTPEKQAVGFFGYRAEIFVDNLLPSFRGKDGSFFRMFSIFFPSATGILSGVNICGDLKDPSGGIPKGTLLAIFWTTVSYLLIAITVAASVVRDASGNLNDSLAFDSSIQCSGLGCKLGWNFDHCEQNHTCSFGLANYFQILTTASGTGHLITIGIFAATLSSALGFLVSAPKIFQCLCKDNIYPYIGFFGKGYGKNQEPLRGYLLMFFIALAFILIGDLNTIAPLISNFFLCSYGLINFSCFHATITKSPGWRPHYHYFSPWTSLFAAFLSFILMFLFTWWAALVTFAIVLSLLGYVTYKKPKINWGTSYQASFYNMALSFSMSLTGVEDHVKNFRPQCLVLTGPPNVRPALVDFVGTFTKNISLMICGNIFMVEEKSRFPQHSTDMLVDWLNQRKVRAFYTSFTADSLTEGTHSLMQASGLGKLKPNTLVLGYKTNWQECKPERLQDYFNTISDAFDSSYGIAVLRMMDGLDIREDLTSGRSSAIDNPAFEADENTKSENEETDRNSDISDDGSNDQVKTVFQTKQGRKTIDIYWISDDGGLTLLVPYLLTRRNRWKKSKIRVFVLGDQETMKEDHKDMKMLLKRFRLEIEDIIVITDVDKPPLAKNLQRYDDSVAPFRLTEEQSRGDLQEFRRLNPWTVSDKDLEAIRPKIERTVRLNEIIKKNSNHAALVVVSLPVPDLNCPSSLYMAWMEALSFGIHCPALLIRGNQENVMTFYCQ

Target ORF information:

RefSeq Version	NM_001135131.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 10, tandem duplicate 3 (slc12a10.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135131.1

ORF Insert Sequence:

ATGAATTTGG AAAAGGTTGA TTATGTGATG GAGCAGGAGC AGGACAAGAG AAAAATGTCA 
ATCTTCAATC CCCTTGATCC CATACCTAAA TTTGAATTTT ATGCTAAAGA CACCTGGACT 
GGACGTATAA AAAGTGCGAG ACCTTCACTA GATGTTCTGC GCAAATCACC TGACCCTGAT 
GATCTTCCTC CACCACCGGA GGATGATGGA CGGCCCAAAG TCAAACTCGT TCGCTTTGGA 
TGGGTTCTGG GAGTCATGAT TCGCTGTATG CTGAATATCT GGGGAGTCAT CATGTTTCTG 
CGGCTGCCGT GGATCACGTC TCAAGCTGGA ATCATTCTGA CATACGTCAT CATCTTCATG 
TCTGTCACCA TCACAACAAT CACAGCGACG TCAGTGTCTG CAATCTCCAC CAATGGGAAG 
GTCTACTCTG GTGGTACGTA CTTCTTGATT TCTCGAAGTC TGGGTCCAGA GCTCGGAGCG 
CCCATTGGTT TGCTCTTCTC GTTCGCCAAT GCTCTCGCCT GCTCGCTCAA CACTGTGGGG 
TTTGCAGAAA CCGTCAGAGA CGTTCTCAAA ATGGTGGACG ATGTGAATGA CGTGCGGATT 
ATTGGGACGA TTACAGTCTG CATCCTCCTC CTCATAACGT TTGCAGGCAT GGCATGGGAG 
GCGAAGGCTC AGATCCTGTT CTTCATTGCG CTGTTGATCT CCTTAGCTAA TTATTTTGTT 
GGGACGCTCC TTCCTCCCAC CCCAGAGAAG CAGGCGGTGG GATTTTTCGG CTACCGCGCT 
GAGATCTTCG TGGATAATCT GCTGCCATCA TTCAGAGGAA AAGACGGCTC TTTCTTCAGG 
ATGTTTTCCA TCTTCTTTCC ATCTGCCACC GGAATTCTGT CTGGCGTCAA CATTTGTGGA 
GATCTCAAGG ATCCCTCCGG TGGGATTCCT AAAGGGACCC TGCTGGCCAT TTTCTGGACC 
ACAGTCAGCT ATTTACTCAT TGCAATTACC GTCGCTGCAA GTGTGGTAAG AGATGCTTCT 
GGCAATCTTA ATGACAGTCT AGCGTTCGAT TCCTCAATCC AGTGCAGTGG CTTGGGCTGT 
AAACTGGGCT GGAATTTTGA CCACTGTGAA CAAAACCACA CCTGTAGCTT TGGACTGGCA 
AACTACTTCC AGATTCTCAC CACTGCCTCT GGAACTGGTC ATCTGATCAC CATCGGGATT 
TTTGCAGCTA CTTTGTCGTC AGCATTGGGT TTCTTGGTTT CTGCACCCAA AATATTTCAG 
TGTCTGTGCA AAGATAACAT TTACCCCTAC ATCGGCTTCT TCGGGAAAGG CTACGGGAAA 
AACCAAGAAC CTCTACGTGG ATATTTGCTG ATGTTCTTCA TTGCGTTGGC CTTCATCCTC 
ATCGGTGACC TGAACACAAT CGCTCCTCTG ATCTCCAACT TCTTCCTCTG CTCTTACGGC 
CTCATCAACT TCAGCTGCTT CCACGCCACG ATCACCAAAT CACCTGGTTG GCGTCCACAT 
TACCACTACT TTTCTCCATG GACGTCTCTG TTTGCTGCTT TCCTCTCATT CATCCTCATG 
TTTTTGTTTA CGTGGTGGGC TGCTCTGGTC ACGTTTGCTA TCGTCCTCTC CCTCTTAGGA 
TACGTCACCT ACAAGAAGCC TAAGATTAAC TGGGGAACGT CCTATCAGGC CAGTTTCTAC 
AACATGGCTC TGTCCTTCTC AATGTCCCTG ACTGGTGTTG AAGACCATGT TAAAAACTTC 
AGGCCTCAGT GTCTGGTTCT GACTGGCCCT CCAAACGTCC GTCCAGCCCT GGTGGACTTT 
GTGGGCACTT TCACTAAAAA CATCAGCCTG ATGATCTGTG GGAACATTTT CATGGTGGAG 
GAGAAGTCCA GGTTTCCACA GCACAGCACT GATATGCTGG TGGACTGGCT GAATCAGAGG 
AAAGTTCGTG CTTTTTACAC GTCCTTCACT GCGGACAGTC TGACAGAGGG AACACACAGT 
CTCATGCAGG CTTCAGGTCT TGGGAAACTG AAGCCAAACA CTCTTGTTTT GGGATATAAG 
ACAAACTGGC AGGAATGTAA ACCAGAGAGA TTACAGGACT ACTTCAACAC CATCAGCGAT 
GCGTTTGACT CCAGCTATGG TATTGCGGTT CTGAGAATGA TGGATGGATT GGACATAAGA 
GAAGATTTGA CTTCAGGCCG ATCGTCTGCA ATTGACAATC CAGCATTTGA GGCAGATGAA 
AATACAAAAT CAGAAAATGA AGAGACCGAC CGAAATTCAG ATATTTCTGA CGATGGCTCC 
AACGATCAGG TCAAAACAGT GTTTCAGACC AAACAGGGCA GGAAGACCAT CGACATCTAC 
TGGATCTCTG ATGATGGAGG TCTGACTCTT CTAGTGCCAT ATTTGCTGAC ACGACGAAAC 
CGCTGGAAGA AGAGCAAAAT CAGAGTGTTT GTCTTAGGAG ATCAAGAGAC CATGAAGGAG 
GACCATAAAG ACATGAAGAT GCTGTTAAAG AGATTCCGGC TAGAGATCGA GGACATCATC 
GTGATCACAG ATGTGGATAA ACCTCCGCTA GCTAAAAATT TGCAGAGGTA TGATGACTCC 
GTCGCCCCCT TCAGGCTGAC TGAGGAACAG AGCAGAGGAG ACCTGCAGGA GTTCAGGAGA 
CTCAATCCGT GGACGGTTTC TGACAAAGAC CTGGAGGCTA TTAGACCCAA GATTGAGAGG 
ACCGTGCGAC TAAATGAAAT CATCAAGAAG AACTCCAACC ACGCTGCTTT GGTTGTAGTT 
TCTCTCCCAG TGCCGGATCT CAACTGTCCC AGTTCTCTCT ATATGGCCTG GATGGAGGCG 
CTGAGTTTTG GGATACACTG TCCTGCCCTG CTCATCCGAG GAAACCAGGA GAACGTCATG 
ACCTTCTACT GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a10.3 ( XM_021477565.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333240.1 Danio rerio slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 (slc12a10.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12476
Clone ID Related Accession (Same CDS sequence)	XM_021477565.1
Accession Version	XM_021477565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAC CCATTGCGCA CATAAGAAAC TTAATCCCGG GTGGTCGACT CCCGGACAGT 
CGACCCCCAG AGAACAACCT TCAGGATGTT ACCATGAATT TGGAAAAGGT TGATTATGTG 
ATGGAGCAGG AGCAGGACAA GAGAAAAATG TCAATCTTCA ATCCCCTTGA TCCCACACCA 
AAATTTGAAT TTTATGCTAA AGACACCTGG ACTGGACGTA TAAAAAGTGC GAGACCTTCA 
CTAGATGTTC TGCGCAAATC ACCTGACCCT GATGATCTTC CTCCACCACC GGAGGATGAT 
GGACGGCCCA AAGTCAAACT CGTTCGCTTT GGATGGGTTC TGGGAGTCAT GATTCGCTGT 
ATGCTGAATA TCTGGGGAGT CATCATGTTT CTGCGGCTGC CGTGGATCAC GTCTCAAGCT 
GGAATCATTC TGACATACGT CATCATCTTC ATGTCTGTCA CCATCACAAC AATCACAGCG 
ACGTCAGTGT CTGCAATCTC CACCAATGGG AAGGTCTACT CTGGTGGTAC GTACTTCTTG 
ATTTCTCGAA GTCTGGGTCC AGAGCTCGGA GCGCCCATTG GTTTGCTCTT CTCGTTCGCC 
AATGCTCTCG CCTGCTCGCT CAACACTGTG GGGTTTGCAG AAACCGTCAG AGACGTTCTC 
AAAGAAAACA ACGCTCAGAT GGTGGACGAT GTGAATGACG TGCGGATTAT TGGGACGATT 
ACAGTCTGCA TCCTCCTCCT CATAACGTTT GCAGGCATGG CATGGGAGGC GAAGGCTCAG 
ATCCTGTTCT TCATTGCGCT GTTGATCTCC TTAGCTAATT ATTTTGTTGG GACGCTCCTT 
CCTCCCACCC CAGAGAAGCA GGCGGTGGGA TTTTTCGGCT ACCGCGCTGA GATCTTCGTG 
GATAATCTGC TGCCATCATT CAGAGGAAAA GACGGCTCTT TCTTCAGGAT GTTTTCCATC 
TTCTTTCCAT CTGCCACCGG AATTCTGTCT GGCGTCAACA TTTGTGGAGA TCTCAAGGAT 
CCCTCAGGTG GGATTCCTAA AGGGACCCTG TTGGCCATTT TCTGGACCAC AGTCAGCTAT 
TTACTCATTG CAATTACCGT CGCTGCAAGT GTGGTAAGAG ATGCTTCTGG CAATCTTAAT 
GACAGTCTAG CGCTCGATTC CTCAATCCAG TGCAGTGGCT TGGGCTGTAA ACTGGGCTGG 
AATTTTGACC ATTGTGAACA AAACCACACC TGTAGCTTTG GACTGGCAAA CTACTTCCAG 
ATTCTCACCA CTGCCTCTGG AACTGGTCAT CTGATCACCA TCGGGATCTT TGCAGCTACT 
TTGTCGTCAG CGTTGGGTTT CTTGGTTTCT GCACCCAAAA TATTTCAGTG TCTGTGCAAA 
GATAACATTT ACCCCTACAT CGGCTTCTTC GGGAAAGGCT ACGGGAAAAA CCAAGAGCCT 
CTACGTGGAT ATTTGCTGAT GTTCTTCATT GCGTTGGCCT TCATCCTCAT CGGTGACCTG 
AACACAATCG CTCCTCTGAT CTCCAACTTC TTCCTCTGCT CTTACGGCCT CATCAACTTC 
AGCTGCTTCC ACGCCACGAT CACCAAATCA CCTGGTTGGC GTCCACATTA CCACTACTTT 
TCTCCATGGA CGTCTCTGTT TGCTGCTTTC CTCTCCTTCA TCCTCATGTT TTTGTTTACG 
TGGTGGGCTG CTCTGGTCAC GTTTGCTATC GTCCTCTCCC TCTTAGGATA CGTCACCTAC 
AAGAAGCCTA AGATTAACTG GGGAACGTCC TATCAGGCCA GTTTCTACAA CATGGCTCTG 
TCCTTCTCAA TGTCCCTGAC TGGTGTTGAA GACCATGTGA AAAACTTCAG GCCTCAGTGT 
CTGGTTCTGA CTGGCCCTCC AAACGTCCGT CCAGCCCTGG TGGACTTTGT GGGCACTTTC 
ACTAAAAACA TCAGCCTGAT GATCTGTGGG AACATTTTCA TGGTGGAGGA GAAGTCCAGG 
TTTCCACAGC ACAGCACTGA TATGCTGGTG GACTGGCTGA ATCAGAGGAA AGTTCGTGCT 
TTTTACACGT CCTTCACTGC GGACAGTCTG ACAGAGGGAA CACACAGTCT CATGCAGGCT 
TCAGGTCTTG GGAAACTGAA GCCGAACACT CTTGTTTTGG GATACAAGAC AAACTGGCAG 
GAATGTAAAC CAGAGAGATT ACAGGACTAC TTCAACACCA TCAGCGATGC GTTTGACTCC 
AGCTATGGTA TTGCGGTTCT GAGAATGATG GATGGATTGG ACATAAGAGA AGATTTGACT 
TCAGGTCGAT CATCTGCAAT TGACAATCCA GCATTTGAGG CAGATGAAAA TACAAAATCA 
GAAAATGAAG AGACCGACCG AAATTCAGAT ATTTCTGACG ATGGCTCCAA CGATCAGGTC 
AAAACAGTGT TTCAGACCAA ACAGGGCAGG AAGACCATCG ACATCTACTG GATCTCTGAT 
GATGGAGGTC TGACTCTTCT AGTGCCGTAT TTGCTGACAC GAAGAAACCG CTGGAAGAAG 
AGCAAAATCA GAGTGTTTGT CTTAGGAGAT CAAGAGACCA TGCAGGAGGA CCATAAAGAC 
ATGAAGATGC TGTTAAAGAG ATTCCGGCTG GAGATCGAGG ACATCATCGT GATCACAGAT 
GTGGATAAAC CTCCGCTAGC GAAAAATTTG CAGAGGTATG ATGACTCCGT CGCCCCCTTC 
AGGCTGACTG AGGAACAGAG CAGAGGAGAC CTGCAGGAGT TCAGGAGACT CAATCCGTGG 
ACAGTTTCTG ACAAAGACCT GGAGGCTATT AGACCCAAGA TTGAGAGGAC CGTGCGACTA 
AATGAAATCA TCAAGAAGAA CTCCAACCAC GCTGCTTTGG TTGTAGTTTC TCTCCCAGTG 
CCGGATCTCA ACTGTCCCAG TTCTCTCTAT ATGGCCTGGA TGGAGGCGCT GAGTTTTGGG 
ATACACTGTC CTGCCCTGCT CATCCGAGGA AACCAGGAGA ACGTCATGAC CTTCTACTGC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333240.1
CDS	114..3119
Translation	MSRPIAHIRNLIPGGRLPDSRPPENNLQDVTMNLEKVDYVMEQEQDKRKMSIFNPLDPTPKFEFYAKDTWTGRIKSARPSLDVLRKSPDPDDLPPPPEDDGRPKVKLVRFGWVLGVMIRCMLNIWGVIMFLRLPWITSQAGIILTYVIIFMSVTITTITATSVSAISTNGKVYSGGTYFLISRSLGPELGAPIGLLFSFANALACSLNTVGFAETVRDVLKENNAQMVDDVNDVRIIGTITVCILLLITFAGMAWEAKAQILFFIALLISLANYFVGTLLPPTPEKQAVGFFGYRAEIFVDNLLPSFRGKDGSFFRMFSIFFPSATGILSGVNICGDLKDPSGGIPKGTLLAIFWTTVSYLLIAITVAASVVRDASGNLNDSLALDSSIQCSGLGCKLGWNFDHCEQNHTCSFGLANYFQILTTASGTGHLITIGIFAATLSSALGFLVSAPKIFQCLCKDNIYPYIGFFGKGYGKNQEPLRGYLLMFFIALAFILIGDLNTIAPLISNFFLCSYGLINFSCFHATITKSPGWRPHYHYFSPWTSLFAAFLSFILMFLFTWWAALVTFAIVLSLLGYVTYKKPKINWGTSYQASFYNMALSFSMSLTGVEDHVKNFRPQCLVLTGPPNVRPALVDFVGTFTKNISLMICGNIFMVEEKSRFPQHSTDMLVDWLNQRKVRAFYTSFTADSLTEGTHSLMQASGLGKLKPNTLVLGYKTNWQECKPERLQDYFNTISDAFDSSYGIAVLRMMDGLDIREDLTSGRSSAIDNPAFEADENTKSENEETDRNSDISDDGSNDQVKTVFQTKQGRKTIDIYWISDDGGLTLLVPYLLTRRNRWKKSKIRVFVLGDQETMQEDHKDMKMLLKRFRLEIEDIIVITDVDKPPLAKNLQRYDDSVAPFRLTEEQSRGDLQEFRRLNPWTVSDKDLEAIRPKIERTVRLNEIIKKNSNHAALVVVSLPVPDLNCPSSLYMAWMEALSFGIHCPALLIRGNQENVMTFYCQ

Target ORF information:

RefSeq Version	XM_021477565.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slc12a10.3 solute carrier family 12 (sodium/potassium/chloride transporters), member 10, tandem duplicate 3 (slc12a10.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477565.1

ORF Insert Sequence:

ATGTCCAGAC CCATTGCGCA CATAAGAAAC TTAATCCCGG GTGGTCGACT CCCGGACAGT 
CGACCCCCAG AGAACAACCT TCAGGATGTT ACCATGAATT TGGAAAAGGT TGATTATGTG 
ATGGAGCAGG AGCAGGACAA GAGAAAAATG TCAATCTTCA ATCCCCTTGA TCCCACACCA 
AAATTTGAAT TTTATGCTAA AGACACCTGG ACTGGACGTA TAAAAAGTGC GAGACCTTCA 
CTAGATGTTC TGCGCAAATC ACCTGACCCT GATGATCTTC CTCCACCACC GGAGGATGAT 
GGACGGCCCA AAGTCAAACT CGTTCGCTTT GGATGGGTTC TGGGAGTCAT GATTCGCTGT 
ATGCTGAATA TCTGGGGAGT CATCATGTTT CTGCGGCTGC CGTGGATCAC GTCTCAAGCT 
GGAATCATTC TGACATACGT CATCATCTTC ATGTCTGTCA CCATCACAAC AATCACAGCG 
ACGTCAGTGT CTGCAATCTC CACCAATGGG AAGGTCTACT CTGGTGGTAC GTACTTCTTG 
ATTTCTCGAA GTCTGGGTCC AGAGCTCGGA GCGCCCATTG GTTTGCTCTT CTCGTTCGCC 
AATGCTCTCG CCTGCTCGCT CAACACTGTG GGGTTTGCAG AAACCGTCAG AGACGTTCTC 
AAAGAAAACA ACGCTCAGAT GGTGGACGAT GTGAATGACG TGCGGATTAT TGGGACGATT 
ACAGTCTGCA TCCTCCTCCT CATAACGTTT GCAGGCATGG CATGGGAGGC GAAGGCTCAG 
ATCCTGTTCT TCATTGCGCT GTTGATCTCC TTAGCTAATT ATTTTGTTGG GACGCTCCTT 
CCTCCCACCC CAGAGAAGCA GGCGGTGGGA TTTTTCGGCT ACCGCGCTGA GATCTTCGTG 
GATAATCTGC TGCCATCATT CAGAGGAAAA GACGGCTCTT TCTTCAGGAT GTTTTCCATC 
TTCTTTCCAT CTGCCACCGG AATTCTGTCT GGCGTCAACA TTTGTGGAGA TCTCAAGGAT 
CCCTCAGGTG GGATTCCTAA AGGGACCCTG TTGGCCATTT TCTGGACCAC AGTCAGCTAT 
TTACTCATTG CAATTACCGT CGCTGCAAGT GTGGTAAGAG ATGCTTCTGG CAATCTTAAT 
GACAGTCTAG CGCTCGATTC CTCAATCCAG TGCAGTGGCT TGGGCTGTAA ACTGGGCTGG 
AATTTTGACC ATTGTGAACA AAACCACACC TGTAGCTTTG GACTGGCAAA CTACTTCCAG 
ATTCTCACCA CTGCCTCTGG AACTGGTCAT CTGATCACCA TCGGGATCTT TGCAGCTACT 
TTGTCGTCAG CGTTGGGTTT CTTGGTTTCT GCACCCAAAA TATTTCAGTG TCTGTGCAAA 
GATAACATTT ACCCCTACAT CGGCTTCTTC GGGAAAGGCT ACGGGAAAAA CCAAGAGCCT 
CTACGTGGAT ATTTGCTGAT GTTCTTCATT GCGTTGGCCT TCATCCTCAT CGGTGACCTG 
AACACAATCG CTCCTCTGAT CTCCAACTTC TTCCTCTGCT CTTACGGCCT CATCAACTTC 
AGCTGCTTCC ACGCCACGAT CACCAAATCA CCTGGTTGGC GTCCACATTA CCACTACTTT 
TCTCCATGGA CGTCTCTGTT TGCTGCTTTC CTCTCCTTCA TCCTCATGTT TTTGTTTACG 
TGGTGGGCTG CTCTGGTCAC GTTTGCTATC GTCCTCTCCC TCTTAGGATA CGTCACCTAC 
AAGAAGCCTA AGATTAACTG GGGAACGTCC TATCAGGCCA GTTTCTACAA CATGGCTCTG 
TCCTTCTCAA TGTCCCTGAC TGGTGTTGAA GACCATGTGA AAAACTTCAG GCCTCAGTGT 
CTGGTTCTGA CTGGCCCTCC AAACGTCCGT CCAGCCCTGG TGGACTTTGT GGGCACTTTC 
ACTAAAAACA TCAGCCTGAT GATCTGTGGG AACATTTTCA TGGTGGAGGA GAAGTCCAGG 
TTTCCACAGC ACAGCACTGA TATGCTGGTG GACTGGCTGA ATCAGAGGAA AGTTCGTGCT 
TTTTACACGT CCTTCACTGC GGACAGTCTG ACAGAGGGAA CACACAGTCT CATGCAGGCT 
TCAGGTCTTG GGAAACTGAA GCCGAACACT CTTGTTTTGG GATACAAGAC AAACTGGCAG 
GAATGTAAAC CAGAGAGATT ACAGGACTAC TTCAACACCA TCAGCGATGC GTTTGACTCC 
AGCTATGGTA TTGCGGTTCT GAGAATGATG GATGGATTGG ACATAAGAGA AGATTTGACT 
TCAGGTCGAT CATCTGCAAT TGACAATCCA GCATTTGAGG CAGATGAAAA TACAAAATCA 
GAAAATGAAG AGACCGACCG AAATTCAGAT ATTTCTGACG ATGGCTCCAA CGATCAGGTC 
AAAACAGTGT TTCAGACCAA ACAGGGCAGG AAGACCATCG ACATCTACTG GATCTCTGAT 
GATGGAGGTC TGACTCTTCT AGTGCCGTAT TTGCTGACAC GAAGAAACCG CTGGAAGAAG 
AGCAAAATCA GAGTGTTTGT CTTAGGAGAT CAAGAGACCA TGCAGGAGGA CCATAAAGAC 
ATGAAGATGC TGTTAAAGAG ATTCCGGCTG GAGATCGAGG ACATCATCGT GATCACAGAT 
GTGGATAAAC CTCCGCTAGC GAAAAATTTG CAGAGGTATG ATGACTCCGT CGCCCCCTTC 
AGGCTGACTG AGGAACAGAG CAGAGGAGAC CTGCAGGAGT TCAGGAGACT CAATCCGTGG 
ACAGTTTCTG ACAAAGACCT GGAGGCTATT AGACCCAAGA TTGAGAGGAC CGTGCGACTA 
AATGAAATCA TCAAGAAGAA CTCCAACCAC GCTGCTTTGG TTGTAGTTTC TCTCCCAGTG 
CCGGATCTCA ACTGTCCCAG TTCTCTCTAT ATGGCCTGGA TGGAGGCGCT GAGTTTTGGG 
ATACACTGTC CTGCCCTGCT CATCCGAGGA AACCAGGAGA ACGTCATGAC CTTCTACTGC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.
International journal of biological sciences11(10)1190-203(2015)
Wang YF,Yan JJ,Tseng YC,Chen RD,Hwang PP

A new model for fish ion regulation: identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes).
Cell and tissue research357(1)225-43(2014 Jul)
Hsu HH,Lin LY,Tseng YC,Horng JL,Hwang PP

Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio).
American journal of physiology. Regulatory, integrative and comparative physiology296(5)R1650-60(2009 May)
Wang YF,Tseng YC,Yan JJ,Hiroi J,Hwang PP

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slc12a10.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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