raph1b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005159955.4	XP_005160012.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X3
XM_009291873.3	XP_009290148.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X2
NM_001365585.1	NP_001352514.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform 1
XM_005159956.3	XP_005160013.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X4
NM_001365589.1	NP_001352518.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform 3
XM_005159954.4	XP_005160011.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
XM_005159955.3	XP_005160012.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X3
XM_009291866.3	XP_009290141.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
XM_009291866.2	XP_009290141.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
XM_005159954.3	XP_005160011.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
XM_005159957.3	XP_005160014.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X5
XM_005159957.4	XP_005160014.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X5
NM_001365587.1	NP_001352516.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform 2
XM_005159956.4	XP_005160013.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X4
XM_009291873.2	XP_009290148.1	ras-associated and pleckstrin homology domains-containing protein 1 isoform X2
NM_001128244.1	NP_001121716.1	ras-associated and pleckstrin homology domains-containing protein 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01678	NM_001128244.1 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa05820	NM_001365585.1 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05821	NM_001365587.1 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05822	NM_001365589.1 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05820	XM_005159954.4 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05820	XM_009291866.3 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05821	XM_005159955.4 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05822	XM_005159956.4 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05823	XM_005159957.4 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa43264	XM_009291873.3 Latest version!	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
ODa05821	XM_005159955.3	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05820	XM_009291866.2	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05820	XM_005159954.3	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa05823	XM_005159957.3	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa05822	XM_005159956.3	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43264	XM_009291873.2	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

raph1b ( NM_001128244.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121716.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), mRNA.

Price & Availability↑

CloneID	ODa01678
Clone ID Related Accession (Same CDS sequence)	NM_001128244.1
Accession Version	NM_001128244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-01
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929045.7. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA708836, SAMEA898404 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGGTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCC 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TCAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TATGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCCCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTAC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATT 
CAGTCACAGC CTCTACCGCC TCCTCCACCC ATAACACCCA TGCAGTCACA TGCTCTACCA 
CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA 
CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT 
CTACCACCTC CTCCACCAAT AATGCCCATG CAGTCAGAGC CTCTACCGCC GCCTGCACAA 
ATCATGCCCA TGCAATCACA TCCTCTTCCA CCACCTCCAC CTTTAGTGCA AATGCAGTCA 
CAGCCCATGC CACCACCTCC TCCCACAGTG TCATTCCAAT CACAGCCTTT ACCACCTCCT 
CCACCCCCTC TCCAGGCTAA TGGCCTTCCC CCTCCTCCAC CTTTAATCAC TAATGGGCTT 
AAGCAGGCTT TTGCCCATAA ATTTCCCAGC AGACCACAGG ATGTATTACC CCCAGCTAAT 
CAAAACAATT TTCCTCCACC TCCCCCACCA CCACCACCTC CACCACTTGC ACCTACGCTC 
CCTCCTTACC AGCATGTTAC TTCAACTTCA AATCCACCAC CACCCCCACC ACCACCACCT 
CTTCCCCCAC CAGCCCCCAT ACATCTACCA AATCAACCTG CGGTTTTACC CAAATTTTTC 
ACTGGTGGAT TTCCACCTCA GAAAGCACCC AAACCTTCAT GTGGCATTCT TCCAGTATCC 
CCAGTAGTTC CAGCACTTCA ACCTCCACCA CCGCCTCCTC CTCCACCTCC TCCTCCACCA 
CCTGCACCAT TGAAGAATCA GCCTCCACCT ACACTGCCCA AGCAGCACAG CCTCTCCAAG 
ACATTGCCCT TCTCCAGTCA AACTTCATCA GTACCGTCTA TGGTTAAGCA GCTTGCTAGT 
CAATTTCCTG TTGCATCTCC TGCATTAACC AATCAAGCAG ATAGTCTCAA AGCTCCCCAG 
TCTCCACCTG CAGTGAAGGT TAAGCCAAAA TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA 
CAGAGGTCAC CTGAATTTCC ACCACCTCCA CCAGACAATG CCATTGGCTT TGCTTCACCA 
ACACCTCCAC CACCACCACC TCCTCCTCTT CCTCCAGGTC CTACTCCTCC TCCTCCCCCT 
CCTCCTCCTC CTCTGATGTC AGGCTCCCCT ATCAAAAAGT CTCCCTCTGG CTCATCTGCA 
GGTGTTAAGA AACCACCGCC AACTCCTCAG CGCAACTCCA GTGTGAAGTA CGATGCCTCA 
ACGGAATACC AGGAGTCCCG GAAGAACTTG GTGAGCAAGT TTAACCCAAG TTCTGCTTCA 
TCAACAACAT CAACCTCCAG CTGTTCACCC TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA 
CCCCCCAAAC CAGGGAAGCT CAATCTGGCA AATCTTCCTT TGGCTTTGCA GAACAAATTC 
AATCAGAACC GCCAGTCAGC GGCAGACTTT CCATCTCCTC CACCCCCAGA AAATGATTTT 
TTCCCACCTC CGCCAATAGA GTCCGACCTC CCTCCACCAC CACCTCTTCC AGGGGATCTA 
AATGGTGTGT CTCCCAAAGT GGCCGTAGTC AATCCTCAGC CTCAGCCTGC TTGGGGCAAA 
AGTTCCCTAA AGAAGACACA ACCTCCAGCA TCACTTCGCT GTAATAACAC TGTTAGAGAG 
TCTCCACCAC TCTCACCACC TTCCATACAC GGGGGGCCAA TTCCCCATTC TCCCAAAGGC 
ACCACACAGC CAAACTTTTT GGAGGACCTT AACAAGACAC TGAAACGCAA GTCTTCAAGA 
GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC ACAATGGATG ACATGGTTTT GCCACCACCT 
CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC 
ATCTCAGGCT ATGCAACACT ACGGCGAGGG CCTCCTCCTG CCCCACCCAA GCGGGACCAC 
AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121716.1
CDS	252..4712
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQVLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYMNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSTHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPIQSQPLPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSRPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPLPPPAPIHLPNQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVVPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQADSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNAIGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	NM_001128244.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128244.1

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGGTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCC 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TCAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TATGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCCCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTAC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATT 
CAGTCACAGC CTCTACCGCC TCCTCCACCC ATAACACCCA TGCAGTCACA TGCTCTACCA 
CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA 
CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT 
CTACCACCTC CTCCACCAAT AATGCCCATG CAGTCAGAGC CTCTACCGCC GCCTGCACAA 
ATCATGCCCA TGCAATCACA TCCTCTTCCA CCACCTCCAC CTTTAGTGCA AATGCAGTCA 
CAGCCCATGC CACCACCTCC TCCCACAGTG TCATTCCAAT CACAGCCTTT ACCACCTCCT 
CCACCCCCTC TCCAGGCTAA TGGCCTTCCC CCTCCTCCAC CTTTAATCAC TAATGGGCTT 
AAGCAGGCTT TTGCCCATAA ATTTCCCAGC AGACCACAGG ATGTATTACC CCCAGCTAAT 
CAAAACAATT TTCCTCCACC TCCCCCACCA CCACCACCTC CACCACTTGC ACCTACGCTC 
CCTCCTTACC AGCATGTTAC TTCAACTTCA AATCCACCAC CACCCCCACC ACCACCACCT 
CTTCCCCCAC CAGCCCCCAT ACATCTACCA AATCAACCTG CGGTTTTACC CAAATTTTTC 
ACTGGTGGAT TTCCACCTCA GAAAGCACCC AAACCTTCAT GTGGCATTCT TCCAGTATCC 
CCAGTAGTTC CAGCACTTCA ACCTCCACCA CCGCCTCCTC CTCCACCTCC TCCTCCACCA 
CCTGCACCAT TGAAGAATCA GCCTCCACCT ACACTGCCCA AGCAGCACAG CCTCTCCAAG 
ACATTGCCCT TCTCCAGTCA AACTTCATCA GTACCGTCTA TGGTTAAGCA GCTTGCTAGT 
CAATTTCCTG TTGCATCTCC TGCATTAACC AATCAAGCAG ATAGTCTCAA AGCTCCCCAG 
TCTCCACCTG CAGTGAAGGT TAAGCCAAAA TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA 
CAGAGGTCAC CTGAATTTCC ACCACCTCCA CCAGACAATG CCATTGGCTT TGCTTCACCA 
ACACCTCCAC CACCACCACC TCCTCCTCTT CCTCCAGGTC CTACTCCTCC TCCTCCCCCT 
CCTCCTCCTC CTCTGATGTC AGGCTCCCCT ATCAAAAAGT CTCCCTCTGG CTCATCTGCA 
GGTGTTAAGA AACCACCGCC AACTCCTCAG CGCAACTCCA GTGTGAAGTA CGATGCCTCA 
ACGGAATACC AGGAGTCCCG GAAGAACTTG GTGAGCAAGT TTAACCCAAG TTCTGCTTCA 
TCAACAACAT CAACCTCCAG CTGTTCACCC TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA 
CCCCCCAAAC CAGGGAAGCT CAATCTGGCA AATCTTCCTT TGGCTTTGCA GAACAAATTC 
AATCAGAACC GCCAGTCAGC GGCAGACTTT CCATCTCCTC CACCCCCAGA AAATGATTTT 
TTCCCACCTC CGCCAATAGA GTCCGACCTC CCTCCACCAC CACCTCTTCC AGGGGATCTA 
AATGGTGTGT CTCCCAAAGT GGCCGTAGTC AATCCTCAGC CTCAGCCTGC TTGGGGCAAA 
AGTTCCCTAA AGAAGACACA ACCTCCAGCA TCACTTCGCT GTAATAACAC TGTTAGAGAG 
TCTCCACCAC TCTCACCACC TTCCATACAC GGGGGGCCAA TTCCCCATTC TCCCAAAGGC 
ACCACACAGC CAAACTTTTT GGAGGACCTT AACAAGACAC TGAAACGCAA GTCTTCAAGA 
GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC ACAATGGATG ACATGGTTTT GCCACCACCT 
CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC 
ATCTCAGGCT ATGCAACACT ACGGCGAGGG CCTCCTCCTG CCCCACCCAA GCGGGACCAC 
AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( NM_001365585.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352514.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa05820
Clone ID Related Accession (Same CDS sequence)	NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
Accession Version	NM_001365585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005426.17. On Sep 12, 2018 this sequence version replaced XM_009291866.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898401 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352514.1
CDS	298..4995
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	NM_001365585.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365585.1

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( NM_001365587.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352516.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 2, mRNA.

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CloneID	ODa05821
Clone ID Related Accession (Same CDS sequence)	XM_005159955.4 , XM_005159955.3 , NM_001365587.1
Accession Version	NM_001365587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005426.17. On Sep 12, 2018 this sequence version replaced XM_005159955.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898401 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC 
TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG 
GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA 
GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC 
AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC 
CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC 
AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA 
CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC 
AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA 
ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT 
CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT 
TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC 
AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG 
TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT 
GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG 
CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA 
AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG 
TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT 
GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC 
CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT 
CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG 
GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT 
CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT 
CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC 
TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA 
CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT 
CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG 
CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT 
CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA 
CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG 
TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC 
ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA 
CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT 
CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG 
CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA 
CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG 
TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC 
CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC 
ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA 
CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA 
AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA 
AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC 
AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA 
CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT 
ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA 
GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC 
AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA 
TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA 
CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT 
CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT 
ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG 
CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG 
GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC 
TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA 
AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT 
CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC 
CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC 
AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA 
TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC 
GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT 
AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC 
ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG 
CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG 
CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352516.1
CDS	298..4908
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	NM_001365587.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365587.1

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC 
TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG 
GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA 
GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC 
AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC 
CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC 
AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA 
CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC 
AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA 
ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT 
CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT 
TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC 
AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG 
TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT 
GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG 
CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA 
AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG 
TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT 
GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC 
CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT 
CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG 
GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT 
CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT 
CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC 
TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA 
CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT 
CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG 
CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT 
CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA 
CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG 
TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC 
ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA 
CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT 
CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG 
CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA 
CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG 
TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC 
CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC 
ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA 
CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA 
AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA 
AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC 
AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA 
CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT 
ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA 
GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC 
AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA 
TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA 
CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT 
CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT 
ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG 
CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG 
GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC 
TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA 
AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT 
CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC 
CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC 
AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA 
TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC 
GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT 
AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC 
ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG 
CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG 
CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( NM_001365589.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352518.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 3, mRNA.

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CloneID	ODa05822
Clone ID Related Accession (Same CDS sequence)	XM_005159956.3 , NM_001365589.1 , XM_005159956.4
Accession Version	NM_001365589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005426.17. On or before Sep 5, 2018 this sequence version replaced NM_001128244.1, XM_005159956.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898404 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG 
CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA 
CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG 
TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA 
CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA 
GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG 
CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA 
ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA 
TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA 
CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC 
CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT 
TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC 
ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA 
CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG 
CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT 
AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT 
CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT 
CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT 
GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT 
CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC 
TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG 
AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC 
CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT 
GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT 
TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC 
CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT 
CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG 
CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT 
AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC 
CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA 
CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG 
GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT 
TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA 
CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352518.1
CDS	298..4833
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	NM_001365589.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365589.1

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG 
CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA 
CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG 
TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA 
CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA 
GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG 
CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA 
ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA 
TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA 
CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC 
CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT 
TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC 
ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA 
CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG 
CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT 
AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT 
CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT 
CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT 
GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT 
CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC 
TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG 
AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC 
CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT 
GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT 
TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC 
CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT 
CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG 
CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT 
AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC 
CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA 
CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG 
GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT 
TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA 
CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159954.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160011.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.

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CloneID	ODa05820
Clone ID Related Accession (Same CDS sequence)	NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
Accession Version	XM_005159954.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159954.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160011.1
CDS	312..5009
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159954.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159954.4

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_009291866.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290141.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05820
Clone ID Related Accession (Same CDS sequence)	NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
Accession Version	XM_009291866.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290141.1
CDS	309..5006
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_009291866.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291866.3

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_009291866.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290141.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05820
Clone ID Related Accession (Same CDS sequence)	NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
Accession Version	XM_009291866.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290141.1
CDS	367..5064
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_009291866.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291866.2

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159954.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160011.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.

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CloneID	ODa05820
Clone ID Related Accession (Same CDS sequence)	NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
Accession Version	XM_005159954.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160011.1
CDS	370..5067
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159954.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159954.3

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG 
ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC 
AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA 
AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG 
AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT 
CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT 
CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG 
TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG 
AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT 
TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT 
AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA 
AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC 
GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC 
CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT 
GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG 
TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC 
TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC 
CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT 
CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG 
AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT 
TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT 
CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA 
CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA 
CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC 
AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT 
CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT 
CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA 
CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG 
ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG 
CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG 
CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG 
TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA 
CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC 
ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG 
CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA 
CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG 
CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA 
AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT 
CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT 
CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT 
GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA 
GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT 
GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA 
CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA 
TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT 
CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG 
AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA 
CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT 
CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT 
GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG 
GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA 
ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC 
CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT 
CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC 
CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT 
GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT 
TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT 
CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC 
ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC 
TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC 
CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC 
TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT 
ACCAAACTTA CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159955.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160012.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.

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CloneID	ODa05821
Clone ID Related Accession (Same CDS sequence)	XM_005159955.4 , XM_005159955.3 , NM_001365587.1
Accession Version	XM_005159955.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159955.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC 
TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG 
GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA 
GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC 
AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC 
CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC 
AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA 
CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC 
AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA 
ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT 
CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT 
TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC 
AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG 
TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT 
GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG 
CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA 
AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG 
TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT 
GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC 
CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT 
CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG 
GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT 
CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT 
CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC 
TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA 
CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT 
CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG 
CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT 
CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA 
CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG 
TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC 
ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA 
CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT 
CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG 
CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA 
CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG 
TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC 
CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC 
ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA 
CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA 
AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA 
AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC 
AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA 
CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT 
ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA 
GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC 
AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA 
TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA 
CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT 
CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT 
ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG 
CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG 
GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC 
TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA 
AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT 
CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC 
CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC 
AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA 
TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC 
GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT 
AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC 
ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG 
CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG 
CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160012.1
CDS	309..4919
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159955.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159955.4

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC 
TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG 
GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA 
GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC 
AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC 
CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC 
AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA 
CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC 
AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA 
ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT 
CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT 
TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC 
AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG 
TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT 
GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG 
CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA 
AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG 
TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT 
GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC 
CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT 
CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG 
GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT 
CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT 
CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC 
TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA 
CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT 
CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG 
CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT 
CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA 
CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG 
TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC 
ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA 
CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT 
CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG 
CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA 
CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG 
TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC 
CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC 
ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA 
CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA 
AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA 
AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC 
AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA 
CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT 
ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA 
GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC 
AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA 
TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA 
CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT 
CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT 
ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG 
CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG 
GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC 
TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA 
AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT 
CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC 
CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC 
AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA 
TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC 
GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT 
AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC 
ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG 
CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG 
CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159955.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160012.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa05821
Clone ID Related Accession (Same CDS sequence)	XM_005159955.4 , XM_005159955.3 , NM_001365587.1
Accession Version	XM_005159955.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC 
TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG 
GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA 
GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC 
AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC 
CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC 
AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA 
CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC 
AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA 
ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT 
CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT 
TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC 
AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG 
TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT 
GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG 
CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA 
AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG 
TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT 
GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC 
CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT 
CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG 
GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT 
CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT 
CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC 
TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA 
CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT 
CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG 
CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT 
CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA 
CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG 
TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC 
ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA 
CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT 
CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG 
CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA 
CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG 
TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC 
CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC 
ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA 
CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA 
AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA 
AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC 
AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA 
CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT 
ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA 
GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC 
AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA 
TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA 
CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT 
CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT 
ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG 
CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG 
GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC 
TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA 
AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT 
CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC 
CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC 
AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA 
TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC 
GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT 
AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC 
ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG 
CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG 
CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160012.1
CDS	367..4977
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHSYLDRETSLILKNIAGKPSHLLTKEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159955.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159955.3

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC 
TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG 
GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA 
GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC 
AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC 
CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC 
AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA 
CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC 
AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA 
ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT 
CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT 
TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC 
AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG 
TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT 
GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG 
CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA 
AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG 
TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT 
GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC 
CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT 
CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG 
GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT 
CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT 
CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC 
TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA 
CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT 
CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG 
CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT 
CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA 
CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG 
TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC 
ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA 
CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT 
CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG 
CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA 
CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG 
TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC 
CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC 
ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA 
CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA 
AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA 
AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC 
AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA 
CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT 
ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA 
GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC 
AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA 
TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA 
CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT 
CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT 
ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG 
CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG 
GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC 
TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA 
AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT 
CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC 
CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC 
AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA 
TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC 
GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT 
AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC 
ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG 
CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG 
CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159956.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160013.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa05822
Clone ID Related Accession (Same CDS sequence)	XM_005159956.3 , NM_001365589.1 , XM_005159956.4
Accession Version	XM_005159956.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG 
CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA 
CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG 
TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA 
CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA 
GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG 
CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA 
ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA 
TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA 
CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC 
CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT 
TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC 
ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA 
CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG 
CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT 
AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT 
CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT 
CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT 
GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT 
CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC 
TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG 
AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC 
CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT 
GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT 
TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC 
CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT 
CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG 
CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT 
AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC 
CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA 
CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG 
GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT 
TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA 
CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160013.1
CDS	366..4901
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159956.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159956.3

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG 
CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA 
CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG 
TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA 
CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA 
GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG 
CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA 
ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA 
TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA 
CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC 
CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT 
TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC 
ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA 
CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG 
CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT 
AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT 
CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT 
CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT 
GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT 
CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC 
TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG 
AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC 
CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT 
GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT 
TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC 
CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT 
CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG 
CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT 
AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC 
CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA 
CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG 
GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT 
TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA 
CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159956.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160013.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.

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CloneID	ODa05822
Clone ID Related Accession (Same CDS sequence)	XM_005159956.3 , NM_001365589.1 , XM_005159956.4
Accession Version	XM_005159956.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159956.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG 
CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA 
CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG 
TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA 
CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA 
GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG 
CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA 
ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA 
TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA 
CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC 
CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT 
TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC 
ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA 
CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG 
CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT 
AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT 
CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT 
CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT 
GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT 
CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC 
TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG 
AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC 
CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT 
GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT 
TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC 
CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT 
CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG 
CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT 
AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC 
CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA 
CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG 
GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT 
TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA 
CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160013.1
CDS	252..4787
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159956.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159956.4

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT 
AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG 
ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA 
TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG 
GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC 
AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT 
CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA 
CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG 
TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT 
GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG 
CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC 
ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA 
TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT 
AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC 
TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA 
CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG 
TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA 
GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA 
AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG 
CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA 
CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT 
CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT 
ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT 
CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA 
CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA 
CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT 
GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT 
CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG 
CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA 
CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG 
TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT 
CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC 
ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA 
CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA 
GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG 
CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA 
ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA 
TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA 
CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC 
CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT 
TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC 
ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA 
CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG 
CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT 
AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT 
CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT 
CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT 
GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT 
CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC 
TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG 
AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC 
CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT 
GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT 
TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC 
CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT 
CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG 
CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT 
AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC 
CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA 
CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG 
GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT 
TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA 
CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159957.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160014.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.

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CloneID	ODa05823
Clone ID Related Accession (Same CDS sequence)	XM_005159957.3 , XM_005159957.4
Accession Version	XM_005159957.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA 
CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC 
AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT 
CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT 
GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG 
ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT 
TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG 
GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG 
AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC 
AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT 
CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT 
CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA 
CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC 
CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC 
ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG 
ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC 
AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA 
AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA 
TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT 
GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC 
CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT 
ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT 
CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG 
CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT 
AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC 
ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC 
CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT 
CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA 
ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA 
CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA 
ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT 
GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA 
CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA 
TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA 
CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG 
GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC 
CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT 
CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT 
GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC 
CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA 
CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA 
CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG 
AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC 
AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA 
TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG 
AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA 
TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA 
CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG 
ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA 
CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG 
TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC 
TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG 
AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG 
TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA 
ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC 
AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG 
ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA 
CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC 
TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA 
GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG 
CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA 
ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160014.1
CDS	1258..5043
Translation	MDVMTACWRGQEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159957.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159957.3

ORF Insert Sequence:

ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA 
CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC 
AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT 
CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT 
GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG 
ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT 
TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG 
GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG 
AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC 
AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT 
CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT 
CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA 
CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC 
CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC 
ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG 
ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC 
AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA 
AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA 
TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT 
GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC 
CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT 
ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT 
CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG 
CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT 
AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC 
ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC 
CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT 
CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA 
ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA 
CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA 
ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT 
GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA 
CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA 
TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA 
CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG 
GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC 
CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT 
CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT 
GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC 
CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA 
CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA 
CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG 
AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC 
AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA 
TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG 
AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA 
TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA 
CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG 
ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA 
CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG 
TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC 
TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG 
AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG 
TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA 
ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC 
AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG 
ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA 
CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC 
TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA 
GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG 
CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA 
ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_005159957.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160014.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa05823
Clone ID Related Accession (Same CDS sequence)	XM_005159957.3 , XM_005159957.4
Accession Version	XM_005159957.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159957.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA 
CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC 
AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT 
CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT 
GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG 
ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT 
TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG 
GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG 
AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC 
AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT 
CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT 
CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA 
CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC 
CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC 
ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG 
ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC 
AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA 
AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA 
TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT 
GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC 
CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT 
ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT 
CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG 
CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT 
AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC 
ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC 
CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT 
CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA 
ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA 
CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA 
ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT 
GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA 
CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA 
TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA 
CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG 
GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC 
CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT 
CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT 
GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC 
CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA 
CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA 
CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG 
AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC 
AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA 
TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG 
AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA 
TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA 
CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG 
ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA 
CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG 
TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC 
TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG 
AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG 
TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA 
ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC 
AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG 
ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA 
CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC 
TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA 
GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG 
CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA 
ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160014.1
CDS	927..4712
Translation	MDVMTACWRGQEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_005159957.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159957.4

ORF Insert Sequence:

ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA 
CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC 
AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT 
CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT 
GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG 
ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT 
TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG 
GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG 
AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC 
AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT 
CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT 
CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA 
CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC 
CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC 
ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG 
ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC 
AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA 
AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA 
TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT 
GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC 
CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT 
ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT 
CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG 
CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT 
AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC 
ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC 
CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT 
CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA 
ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA 
CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA 
ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT 
GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA 
CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA 
TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA 
CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG 
GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC 
CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT 
CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT 
GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC 
CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA 
CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA 
CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG 
AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC 
AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA 
TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG 
AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA 
TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA 
CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG 
ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA 
CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG 
TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC 
TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG 
AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG 
TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA 
ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC 
AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG 
ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA 
CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC 
TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA 
GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG 
CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA 
ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_009291873.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290148.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa43264
Clone ID Related Accession (Same CDS sequence)	XM_009291873.3 , XM_009291873.2
Accession Version	XM_009291873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG 
AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT 
GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG 
CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG 
GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA 
GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT 
GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT 
GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG 
TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG 
AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG 
GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG 
GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG 
ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC 
CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG 
GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC 
AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA 
GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT 
TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG 
CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT 
CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA 
AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA 
GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT 
TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG 
CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA 
GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC 
ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG 
GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC 
CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA 
CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA 
ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA 
CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA 
CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT 
CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA 
ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA 
CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT 
CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT 
AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT 
AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA 
CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT 
ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC 
ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT 
CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT 
CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT 
CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT 
CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT 
CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG 
GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT 
CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA 
CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG 
TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG 
CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC 
CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC 
AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG 
CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA 
GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA 
GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA 
GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA 
CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA 
CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT 
TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG 
GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG 
GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA 
CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290148.1
CDS	309..4931
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_009291873.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291873.3

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG 
AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT 
GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG 
CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG 
GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA 
GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT 
GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT 
GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG 
TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG 
AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG 
GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG 
GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG 
ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC 
CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG 
GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC 
AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA 
GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT 
TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG 
CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT 
CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA 
AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA 
GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT 
TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG 
CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA 
GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC 
ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG 
GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC 
CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA 
CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA 
ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA 
CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA 
CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT 
CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA 
ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA 
CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT 
CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT 
AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT 
AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA 
CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT 
ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC 
ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT 
CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT 
CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT 
CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT 
CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT 
CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG 
GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT 
CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA 
CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG 
TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG 
CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC 
CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC 
AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG 
CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA 
GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA 
GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA 
GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA 
CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA 
CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT 
TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG 
GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG 
GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA 
CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raph1b ( XM_009291873.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290148.1 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.

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CloneID	ODa43264
Clone ID Related Accession (Same CDS sequence)	XM_009291873.3 , XM_009291873.2
Accession Version	XM_009291873.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-associated and pleckstrin homology domains-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG 
AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT 
GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG 
CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG 
GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA 
GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT 
GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT 
GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG 
TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG 
AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG 
GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG 
GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG 
ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC 
CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG 
GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC 
AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA 
GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT 
TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG 
CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT 
CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA 
AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA 
GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT 
TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG 
CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA 
GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC 
ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG 
GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC 
CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA 
CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA 
ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA 
CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA 
CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT 
CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA 
ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA 
CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT 
CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT 
AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT 
AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA 
CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT 
ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC 
ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT 
CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT 
CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT 
CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT 
CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT 
CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG 
GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT 
CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA 
CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG 
TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG 
CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC 
CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC 
AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG 
CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA 
GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA 
GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA 
GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA 
CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA 
CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT 
TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG 
GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG 
GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA 
CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290148.1
CDS	367..4989
Translation	MEQVSDDELDHAAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMADLCSIEQELSTIGKPISKTLDGKSKQRPTGRSASAKHSGGGSSGGSTSSSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNSHHRRTGSVGTVSDQEVRSIGHSSRSSVNSASASSMDSLDIRGQENETQSQNQSQTSTEHVLLRRANGKTTRRHLDFTSQEGEVDQSTEEQAAKAKAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVETIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMADRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLVCFLQLDHVNVYYGQDYRSKYKAPTDYCLALKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYVNYQEAMKRTEAAYDWSSLSSTSIKSGTSSVSIPESQSNHSSQTDSGMSDGTSSSHARSQSVVSSIFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSHHVSQQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPHHATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPPPPASMPPPGSAMAVLKLGPPAAAVIPQISPSPPSPPPPPPPPPIMPVQSQPQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQSHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKFPSTPQDVLPPANQNNFPPPPPPPPPPPLAPTLPPYQHVTSTSNPPPPPPPPPPPPPAPIHLANQPAVLPKFFTGGFPPQKAPKPSCGILPVSPVAPALQPPPPPPPPPPPPPPAPLKNQPPPTLPKQHSLSKTLPFSSQTSSVPSMVKQLASQFPVASPALTNQTDSLKAPQSPPAVKVKPKWQPVGQGQQQQRSPEFPPPPPDNALGFASPTPPPPPPPPLPPGPTPPPPPPPPPLMSGSPIKKSPSGSSAGVKKPPPTPQRNSSVKYDASTEYQESRKNLVSKFNPSSASSTTSTSSCSPSKELSAGPQAPPKPGKLNLANLPLALQNKFNQNRQSAADFPSPPPPENDFFPPPPIESDLPPPPPLPGDLNGVSPKVAVVNPQPQPAWGKSSLKKTQPPASLRCNNTVRESPPLSPPSIHGGPIPHSPKGTTQPNFLEDLNKTLKRKSSRVSGDKVDPVATMDDMVLPPPPPELLQDQQRLSGSGFMSGNISGYATLRRGPPPAPPKRDHSTKLTN

Target ORF information:

RefSeq Version	XM_009291873.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291873.2

ORF Insert Sequence:

ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG 
AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC 
CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT 
GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC 
ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA 
CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT 
ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT 
TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT 
GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT 
TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT 
TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC 
CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA 
GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC 
TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG 
AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT 
GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG 
CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG 
GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA 
GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT 
GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT 
GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG 
TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG 
AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG 
GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG 
GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG 
ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC 
CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG 
GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC 
AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA 
GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT 
TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG 
CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT 
CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA 
AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA 
GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT 
TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG 
CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA 
GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC 
ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG 
GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC 
CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA 
CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA 
ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA 
CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA 
CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT 
CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA 
ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA 
CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT 
CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT 
AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT 
AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA 
CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT 
ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC 
ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT 
CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT 
CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT 
CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT 
CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT 
CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG 
GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT 
CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA 
CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG 
TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG 
CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC 
CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC 
AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG 
CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA 
GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA 
GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA 
GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA 
CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA 
CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT 
TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG 
GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG 
GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA 
CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	raph1b
Entrez Gene ID	560319
Full Name	Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b
Synonyms	si:dkey-1h24.3
General protein information	Preferred Names Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b Names ras-associated and pleckstrin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Rattus norvegicus (Norway rat)	Raph1	NP_001102268.2
Danio rerio (zebrafish)	si:dkey-1h24.3	NP_001121716.1
Danio rerio (zebrafish)	raph1	XP_005167562.1
Homo sapiens (human)	RAPH1	NP_998754.1
Pan troglodytes (chimpanzee)	RAPH1	XP_516044.3
Canis lupus familiaris (dog)	RAPH1	XP_005640794.1
Gallus gallus (chicken)	RAPH1	XP_421961.4
	LOC705065	XP_002808066.1
Bos taurus (cattle)	RAPH1	XP_003585810.1
Mus musculus (house mouse)	Raph1	NP_001038978.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

raph1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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