si:ch73-190m4.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch73-190m4.1
Entrez Gene ID	559981
Full Name	si:ch73-190m4.1
General protein information	Preferred Names si:ch73-190m4.1 Names probable E3 ubiquitin-protein ligase HERC4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017355897.1	XP_017211386.1	probable E3 ubiquitin-protein ligase HERC4
XM_017355897.2	XP_017211386.1	probable E3 ubiquitin-protein ligase HERC4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch73-190m4.1	XP_688463.5
Danio rerio (zebrafish)	zgc:163136	NP_001092233.1
Danio rerio (zebrafish)	si:ch211-281g13.4	NP_001139174.1
Gallus gallus (chicken)	LOC422513	XP_420476.4
Danio rerio (zebrafish)	si:ch211-281g13.5	NP_001139103.1

Gene Ontology

The following si:ch73-190m4.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch73-190m4.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53779	XM_017355897.2 Latest version!	Danio rerio si:ch73-190m4.1 (si:ch73-190m4.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa53779	XM_017355897.1	Danio rerio si:ch73-190m4.1 (si:ch73-190m4.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch73-190m4.1 ( XM_017355897.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211386.1 Danio rerio si:ch73-190m4.1 (si:ch73-190m4.1), mRNA.

Price & Availability↑

CloneID	ODa53779
Clone ID Related Accession (Same CDS sequence)	XM_017355897.1 , XM_017355897.2
Accession Version	XM_017355897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATTCCTC TTAAAGGTCT GGAGAACAGG CAGGTGATTC AGATCGCATG TGGAGACCAG 
CATTCAATGG CGCTAACCAA CGATGGTCAG TTGTTTGTTT GGGGCGAGAA CGCTCTTGGT 
CAGCTGGGTT TGAGGAAAGA GCAGGCCGGC ACTCAGTCTC CTCGACACCT CCAGAGTCTG 
TGTGGGATCC CAGTGGCTCA GATCAGCGCT GGAGGAAACC ACAGCTTTGT GTTGTCTCTG 
TCAGGAGTCG TGTTTGGGTG GGGGGGCAAC TCTGCTGGAC AGCTGGGTCT CGGAGACACT 
ACAGACAGAT TCGTCCCAAC AGTTGTAAAT AGTCTTAAAC GAAAGAAGAT TGTGTCCATC 
TCATGTGGAG GAGAACACAC TGCAGCTCTA GCAAAGGGAG GAACAGTGTT CACATTTGGA 
TCAGGAGGTT TCGGTCAGCT TGGGCACAAC TCATTTAAAG ATGAACATCA TCCACGACTG 
GTTGCTGAAC TGTGGGGATC TAAAGTGTCA CAGGTCACAT GTGGGAGAAA TCACACACTT 
GTATCGACTG CATCACCAAA GCTGATCTAC TCATTTGGAT GTGGGATGCA AGGGCAGCTG 
GGAAATGGAG AACAGGTCAA GCAGTCTGTG CCGTTACCAG TAGACCAGCC AACTGAATAT 
AATGACGAAT ATACAATTCA AAAACTTGTC GCGGGGGAGA ATCATTCGTT TGCCTTGTAT 
TTCAAAGAAC TTGGAAATGA GGCTAATCCA GATCCAACCA GAGGGATTAT GACATTTGAT 
AACGGAATGA TTGAACAATG CGAGTGTGGA AAAGATCCAC GGAAAGCGAT AAAGGACTTG 
AAAAAAGTGT TCTCCTCTGC TGCATCTCTA AATGGAAGTT TTCTCAAAAC AAGGCATGAT 
GGTCATTATC AGACGTCTGA AGAGTATTGT GGTTTGGATT TTGGTCTGGT TGAATCTTCC 
ATTGCAACTT TATCAAGGAA TGAAAGGCTT ATCTCAACGG TTTTGAAAGT GCTCCAAGAG 
TCACTGTTGC CAACTCTAAA TCCAAATCCA ACTGGTGAGG AATCACTGAG ACTCTATATT 
CTCCTGCCTG AGCTGATCAG CCATCTCAAG AGTGAACAAA AAGCAGAACT TACTGAAGCT 
CTGGCCTCCA AAATACTTAA TCTAAGTCCT GCTGCTCTGA GAGTGTTAGA GATCTACTGG 
TCCAAACTCC CTGATGACTG GCTTAAAAAC TTGGTTAAGT TATTCAAAAA GGCATCAGCT 
AAATTCTTTG GGAGGATCAT TGTCGAGAAC GTGAACAATG TGGCTAAAAC ACACATGCTA 
AAATTTGTGG AGATTCTTCA AAGGGTCTAT CAGGTCTGCT GCAGCGCCAA CAGAAATATC 
AAGAAGAGGT ATTTCATCAT TCATGAGGTC AACAATCTCC TAGATGTGCT ACAAACTATG 
AATGCAGATG TTAATAACAA CTGGCCTTGG TTTAACCTTA CTGGACAAAT CCATGCCCTG 
GAGGCACAAC GAAATGCGTA TGAAGAAACT TTTAGAAAGC TGACATCATT TCCATGCATC 
TTTAATCTGG AGGCCAAATG TAGCTATATG AGGAACAGAG TATGGAAGGG TCGTTTTGAG 
CTGACTGTGA GACGCACAGC ACTGCTAGAG GATTGTTTAT GTCTACTAAA CCCAGGCACC 
CAACAGCATC TCAGAGATAA ATGGCTGTCG ATTCTTTTCA CTGAGAACGT GAGCAAAAGA 
ACAGACGTCC ACAAGAGGGA CTTCTTTCTC AACGTGTTTA AGGAGCTTTG TGATCCAGCT 
TCTCAGCTCT TCATGTATAA CGACAACGAA ACTATGATCT GGTTCCCCGC GAAGCTCACC 
GTGGAGAAGA AGAAGTACTT CCTGTTTGGG ATTCTGTGTG GCTTGGCCTT CAACAACAGC 
TCTGTAGTCA ATCTGCCTTT CCCTTTAGCC CTGTTCAAGA AACTTCTCAA CATCAAACCA 
TCTCTGGAAG ATTTTATAGA GTTTAATCCA GTCCATGGCA GGAGTTTACA GTACATCCTA 
GAGTACAGTA ATGATGTTCT GGAGGAATCG TCCTTGCCCT TAACGATAAT ATGGAGTGGA 
TCAGAGCTGG ACCTGGACCC TGCTGAGCCA GAACAGCACA TCACGACTTC TAACAGAACT 
AAGTTTGTGG ATGAATATGT GGATCACATC TTCAATCAAT CAGTGAAGGA GGCGTTTGAA 
GAATTTAGAA GAGGCTTCTT TAGAGGATGT GAGAAACGCT TGGTGGAGAT GTTTGAACCA 
GAAGAACTGA GGGGGGTGCT GGTGGGCAAT GAGGAATATG ACTGGAATAT ACTCAAGCAG 
AACACTACAT ATGAAGGCGT ATTTAATGCG GATCATCCAG TCATAATCTC TTTCTGGGAG 
GTGTTTGATG ATCTGACACC AAATGAGAAG AAGTCCTTCT TGTTGTTTTT GACGGGGTTT 
GAGAGAGTGC CTATTCTGGG CATGAGCGCG GTGAAGATGA GAGTGACACA TTTGGCTAAC 
TCAACTGAGG ATCATCTGCC AGAGACTCTC ACCTGCCATT CTCTTCTGCA GCTTCCTAAA 
TATGAGAAAA GGGAAACCCT CAGAGCCAAA GTTATTGAGG CCATCAGCCA CAAGAGGGGG 
TTCTGGGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211386.1
CDS	1..2655
Translation	MIPLKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPRHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGGNSAGQLGLGDTTDRFVPTVVNSLKRKKIVSISCGGEHTAALAKGGTVFTFGSGGFGQLGHNSFKDEHHPRLVAELWGSKVSQVTCGRNHTLVSTASPKLIYSFGCGMQGQLGNGEQVKQSVPLPVDQPTEYNDEYTIQKLVAGENHSFALYFKELGNEANPDPTRGIMTFDNGMIEQCECGKDPRKAIKDLKKVFSSAASLNGSFLKTRHDGHYQTSEEYCGLDFGLVESSIATLSRNERLISTVLKVLQESLLPTLNPNPTGEESLRLYILLPELISHLKSEQKAELTEALASKILNLSPAALRVLEIYWSKLPDDWLKNLVKLFKKASAKFFGRIIVENVNNVAKTHMLKFVEILQRVYQVCCSANRNIKKRYFIIHEVNNLLDVLQTMNADVNNNWPWFNLTGQIHALEAQRNAYEETFRKLTSFPCIFNLEAKCSYMRNRVWKGRFELTVRRTALLEDCLCLLNPGTQQHLRDKWLSILFTENVSKRTDVHKRDFFLNVFKELCDPASQLFMYNDNETMIWFPAKLTVEKKKYFLFGILCGLAFNNSSVVNLPFPLALFKKLLNIKPSLEDFIEFNPVHGRSLQYILEYSNDVLEESSLPLTIIWSGSELDLDPAEPEQHITTSNRTKFVDEYVDHIFNQSVKEAFEEFRRGFFRGCEKRLVEMFEPEELRGVLVGNEEYDWNILKQNTTYEGVFNADHPVIISFWEVFDDLTPNEKKSFLLFLTGFERVPILGMSAVKMRVTHLANSTEDHLPETLTCHSLLQLPKYEKRETLRAKVIEAISHKRGFWEE

Target ORF information:

RefSeq Version	XM_017355897.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-190m4.1 (si:ch73-190m4.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355897.1

ORF Insert Sequence:

ATGATTCCTC TTAAAGGTCT GGAGAACAGG CAGGTGATTC AGATCGCATG TGGAGACCAG 
CATTCAATGG CGCTAACCAA CGATGGTCAG TTGTTTGTTT GGGGCGAGAA CGCTCTTGGT 
CAGCTGGGTT TGAGGAAAGA GCAGGCCGGC ACTCAGTCTC CTCGACACCT CCAGAGTCTG 
TGTGGGATCC CAGTGGCTCA GATCAGCGCT GGAGGAAACC ACAGCTTTGT GTTGTCTCTG 
TCAGGAGTCG TGTTTGGGTG GGGGGGCAAC TCTGCTGGAC AGCTGGGTCT CGGAGACACT 
ACAGACAGAT TCGTCCCAAC AGTTGTAAAT AGTCTTAAAC GAAAGAAGAT TGTGTCCATC 
TCATGTGGAG GAGAACACAC TGCAGCTCTA GCAAAGGGAG GAACAGTGTT CACATTTGGA 
TCAGGAGGTT TCGGTCAGCT TGGGCACAAC TCATTTAAAG ATGAACATCA TCCACGACTG 
GTTGCTGAAC TGTGGGGATC TAAAGTGTCA CAGGTCACAT GTGGGAGAAA TCACACACTT 
GTATCGACTG CATCACCAAA GCTGATCTAC TCATTTGGAT GTGGGATGCA AGGGCAGCTG 
GGAAATGGAG AACAGGTCAA GCAGTCTGTG CCGTTACCAG TAGACCAGCC AACTGAATAT 
AATGACGAAT ATACAATTCA AAAACTTGTC GCGGGGGAGA ATCATTCGTT TGCCTTGTAT 
TTCAAAGAAC TTGGAAATGA GGCTAATCCA GATCCAACCA GAGGGATTAT GACATTTGAT 
AACGGAATGA TTGAACAATG CGAGTGTGGA AAAGATCCAC GGAAAGCGAT AAAGGACTTG 
AAAAAAGTGT TCTCCTCTGC TGCATCTCTA AATGGAAGTT TTCTCAAAAC AAGGCATGAT 
GGTCATTATC AGACGTCTGA AGAGTATTGT GGTTTGGATT TTGGTCTGGT TGAATCTTCC 
ATTGCAACTT TATCAAGGAA TGAAAGGCTT ATCTCAACGG TTTTGAAAGT GCTCCAAGAG 
TCACTGTTGC CAACTCTAAA TCCAAATCCA ACTGGTGAGG AATCACTGAG ACTCTATATT 
CTCCTGCCTG AGCTGATCAG CCATCTCAAG AGTGAACAAA AAGCAGAACT TACTGAAGCT 
CTGGCCTCCA AAATACTTAA TCTAAGTCCT GCTGCTCTGA GAGTGTTAGA GATCTACTGG 
TCCAAACTCC CTGATGACTG GCTTAAAAAC TTGGTTAAGT TATTCAAAAA GGCATCAGCT 
AAATTCTTTG GGAGGATCAT TGTCGAGAAC GTGAACAATG TGGCTAAAAC ACACATGCTA 
AAATTTGTGG AGATTCTTCA AAGGGTCTAT CAGGTCTGCT GCAGCGCCAA CAGAAATATC 
AAGAAGAGGT ATTTCATCAT TCATGAGGTC AACAATCTCC TAGATGTGCT ACAAACTATG 
AATGCAGATG TTAATAACAA CTGGCCTTGG TTTAACCTTA CTGGACAAAT CCATGCCCTG 
GAGGCACAAC GAAATGCGTA TGAAGAAACT TTTAGAAAGC TGACATCATT TCCATGCATC 
TTTAATCTGG AGGCCAAATG TAGCTATATG AGGAACAGAG TATGGAAGGG TCGTTTTGAG 
CTGACTGTGA GACGCACAGC ACTGCTAGAG GATTGTTTAT GTCTACTAAA CCCAGGCACC 
CAACAGCATC TCAGAGATAA ATGGCTGTCG ATTCTTTTCA CTGAGAACGT GAGCAAAAGA 
ACAGACGTCC ACAAGAGGGA CTTCTTTCTC AACGTGTTTA AGGAGCTTTG TGATCCAGCT 
TCTCAGCTCT TCATGTATAA CGACAACGAA ACTATGATCT GGTTCCCCGC GAAGCTCACC 
GTGGAGAAGA AGAAGTACTT CCTGTTTGGG ATTCTGTGTG GCTTGGCCTT CAACAACAGC 
TCTGTAGTCA ATCTGCCTTT CCCTTTAGCC CTGTTCAAGA AACTTCTCAA CATCAAACCA 
TCTCTGGAAG ATTTTATAGA GTTTAATCCA GTCCATGGCA GGAGTTTACA GTACATCCTA 
GAGTACAGTA ATGATGTTCT GGAGGAATCG TCCTTGCCCT TAACGATAAT ATGGAGTGGA 
TCAGAGCTGG ACCTGGACCC TGCTGAGCCA GAACAGCACA TCACGACTTC TAACAGAACT 
AAGTTTGTGG ATGAATATGT GGATCACATC TTCAATCAAT CAGTGAAGGA GGCGTTTGAA 
GAATTTAGAA GAGGCTTCTT TAGAGGATGT GAGAAACGCT TGGTGGAGAT GTTTGAACCA 
GAAGAACTGA GGGGGGTGCT GGTGGGCAAT GAGGAATATG ACTGGAATAT ACTCAAGCAG 
AACACTACAT ATGAAGGCGT ATTTAATGCG GATCATCCAG TCATAATCTC TTTCTGGGAG 
GTGTTTGATG ATCTGACACC AAATGAGAAG AAGTCCTTCT TGTTGTTTTT GACGGGGTTT 
GAGAGAGTGC CTATTCTGGG CATGAGCGCG GTGAAGATGA GAGTGACACA TTTGGCTAAC 
TCAACTGAGG ATCATCTGCC AGAGACTCTC ACCTGCCATT CTCTTCTGCA GCTTCCTAAA 
TATGAGAAAA GGGAAACCCT CAGAGCCAAA GTTATTGAGG CCATCAGCCA CAAGAGGGGG 
TTCTGGGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-190m4.1 ( XM_017355897.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211386.1 Danio rerio si:ch73-190m4.1 (si:ch73-190m4.1), mRNA.

Price & Availability↑

CloneID	ODa53779
Clone ID Related Accession (Same CDS sequence)	XM_017355897.1 , XM_017355897.2
Accession Version	XM_017355897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATTCCTC TTAAAGGTCT GGAGAACAGG CAGGTGATTC AGATCGCATG TGGAGACCAG 
CATTCAATGG CGCTAACCAA CGATGGTCAG TTGTTTGTTT GGGGCGAGAA CGCTCTTGGT 
CAGCTGGGTT TGAGGAAAGA GCAGGCCGGC ACTCAGTCTC CTCGACACCT CCAGAGTCTG 
TGTGGGATCC CAGTGGCTCA GATCAGCGCT GGAGGAAACC ACAGCTTTGT GTTGTCTCTG 
TCAGGAGTCG TGTTTGGGTG GGGGGGCAAC TCTGCTGGAC AGCTGGGTCT CGGAGACACT 
ACAGACAGAT TCGTCCCAAC AGTTGTAAAT AGTCTTAAAC GAAAGAAGAT TGTGTCCATC 
TCATGTGGAG GAGAACACAC TGCAGCTCTA GCAAAGGGAG GAACAGTGTT CACATTTGGA 
TCAGGAGGTT TCGGTCAGCT TGGGCACAAC TCATTTAAAG ATGAACATCA TCCACGACTG 
GTTGCTGAAC TGTGGGGATC TAAAGTGTCA CAGGTCACAT GTGGGAGAAA TCACACACTT 
GTATCGACTG CATCACCAAA GCTGATCTAC TCATTTGGAT GTGGGATGCA AGGGCAGCTG 
GGAAATGGAG AACAGGTCAA GCAGTCTGTG CCGTTACCAG TAGACCAGCC AACTGAATAT 
AATGACGAAT ATACAATTCA AAAACTTGTC GCGGGGGAGA ATCATTCGTT TGCCTTGTAT 
TTCAAAGAAC TTGGAAATGA GGCTAATCCA GATCCAACCA GAGGGATTAT GACATTTGAT 
AACGGAATGA TTGAACAATG CGAGTGTGGA AAAGATCCAC GGAAAGCGAT AAAGGACTTG 
AAAAAAGTGT TCTCCTCTGC TGCATCTCTA AATGGAAGTT TTCTCAAAAC AAGGCATGAT 
GGTCATTATC AGACGTCTGA AGAGTATTGT GGTTTGGATT TTGGTCTGGT TGAATCTTCC 
ATTGCAACTT TATCAAGGAA TGAAAGGCTT ATCTCAACGG TTTTGAAAGT GCTCCAAGAG 
TCACTGTTGC CAACTCTAAA TCCAAATCCA ACTGGTGAGG AATCACTGAG ACTCTATATT 
CTCCTGCCTG AGCTGATCAG CCATCTCAAG AGTGAACAAA AAGCAGAACT TACTGAAGCT 
CTGGCCTCCA AAATACTTAA TCTAAGTCCT GCTGCTCTGA GAGTGTTAGA GATCTACTGG 
TCCAAACTCC CTGATGACTG GCTTAAAAAC TTGGTTAAGT TATTCAAAAA GGCATCAGCT 
AAATTCTTTG GGAGGATCAT TGTCGAGAAC GTGAACAATG TGGCTAAAAC ACACATGCTA 
AAATTTGTGG AGATTCTTCA AAGGGTCTAT CAGGTCTGCT GCAGCGCCAA CAGAAATATC 
AAGAAGAGGT ATTTCATCAT TCATGAGGTC AACAATCTCC TAGATGTGCT ACAAACTATG 
AATGCAGATG TTAATAACAA CTGGCCTTGG TTTAACCTTA CTGGACAAAT CCATGCCCTG 
GAGGCACAAC GAAATGCGTA TGAAGAAACT TTTAGAAAGC TGACATCATT TCCATGCATC 
TTTAATCTGG AGGCCAAATG TAGCTATATG AGGAACAGAG TATGGAAGGG TCGTTTTGAG 
CTGACTGTGA GACGCACAGC ACTGCTAGAG GATTGTTTAT GTCTACTAAA CCCAGGCACC 
CAACAGCATC TCAGAGATAA ATGGCTGTCG ATTCTTTTCA CTGAGAACGT GAGCAAAAGA 
ACAGACGTCC ACAAGAGGGA CTTCTTTCTC AACGTGTTTA AGGAGCTTTG TGATCCAGCT 
TCTCAGCTCT TCATGTATAA CGACAACGAA ACTATGATCT GGTTCCCCGC GAAGCTCACC 
GTGGAGAAGA AGAAGTACTT CCTGTTTGGG ATTCTGTGTG GCTTGGCCTT CAACAACAGC 
TCTGTAGTCA ATCTGCCTTT CCCTTTAGCC CTGTTCAAGA AACTTCTCAA CATCAAACCA 
TCTCTGGAAG ATTTTATAGA GTTTAATCCA GTCCATGGCA GGAGTTTACA GTACATCCTA 
GAGTACAGTA ATGATGTTCT GGAGGAATCG TCCTTGCCCT TAACGATAAT ATGGAGTGGA 
TCAGAGCTGG ACCTGGACCC TGCTGAGCCA GAACAGCACA TCACGACTTC TAACAGAACT 
AAGTTTGTGG ATGAATATGT GGATCACATC TTCAATCAAT CAGTGAAGGA GGCGTTTGAA 
GAATTTAGAA GAGGCTTCTT TAGAGGATGT GAGAAACGCT TGGTGGAGAT GTTTGAACCA 
GAAGAACTGA GGGGGGTGCT GGTGGGCAAT GAGGAATATG ACTGGAATAT ACTCAAGCAG 
AACACTACAT ATGAAGGCGT ATTTAATGCG GATCATCCAG TCATAATCTC TTTCTGGGAG 
GTGTTTGATG ATCTGACACC AAATGAGAAG AAGTCCTTCT TGTTGTTTTT GACGGGGTTT 
GAGAGAGTGC CTATTCTGGG CATGAGCGCG GTGAAGATGA GAGTGACACA TTTGGCTAAC 
TCAACTGAGG ATCATCTGCC AGAGACTCTC ACCTGCCATT CTCTTCTGCA GCTTCCTAAA 
TATGAGAAAA GGGAAACCCT CAGAGCCAAA GTTATTGAGG CCATCAGCCA CAAGAGGGGG 
TTCTGGGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211386.1
CDS	1..2655
Translation	MIPLKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPRHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGGNSAGQLGLGDTTDRFVPTVVNSLKRKKIVSISCGGEHTAALAKGGTVFTFGSGGFGQLGHNSFKDEHHPRLVAELWGSKVSQVTCGRNHTLVSTASPKLIYSFGCGMQGQLGNGEQVKQSVPLPVDQPTEYNDEYTIQKLVAGENHSFALYFKELGNEANPDPTRGIMTFDNGMIEQCECGKDPRKAIKDLKKVFSSAASLNGSFLKTRHDGHYQTSEEYCGLDFGLVESSIATLSRNERLISTVLKVLQESLLPTLNPNPTGEESLRLYILLPELISHLKSEQKAELTEALASKILNLSPAALRVLEIYWSKLPDDWLKNLVKLFKKASAKFFGRIIVENVNNVAKTHMLKFVEILQRVYQVCCSANRNIKKRYFIIHEVNNLLDVLQTMNADVNNNWPWFNLTGQIHALEAQRNAYEETFRKLTSFPCIFNLEAKCSYMRNRVWKGRFELTVRRTALLEDCLCLLNPGTQQHLRDKWLSILFTENVSKRTDVHKRDFFLNVFKELCDPASQLFMYNDNETMIWFPAKLTVEKKKYFLFGILCGLAFNNSSVVNLPFPLALFKKLLNIKPSLEDFIEFNPVHGRSLQYILEYSNDVLEESSLPLTIIWSGSELDLDPAEPEQHITTSNRTKFVDEYVDHIFNQSVKEAFEEFRRGFFRGCEKRLVEMFEPEELRGVLVGNEEYDWNILKQNTTYEGVFNADHPVIISFWEVFDDLTPNEKKSFLLFLTGFERVPILGMSAVKMRVTHLANSTEDHLPETLTCHSLLQLPKYEKRETLRAKVIEAISHKRGFWEE

Target ORF information:

RefSeq Version	XM_017355897.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-190m4.1 (si:ch73-190m4.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355897.2

ORF Insert Sequence:

ATGATTCCTC TTAAAGGTCT GGAGAACAGG CAGGTGATTC AGATCGCATG TGGAGACCAG 
CATTCAATGG CGCTAACCAA CGATGGTCAG TTGTTTGTTT GGGGCGAGAA CGCTCTTGGT 
CAGCTGGGTT TGAGGAAAGA GCAGGCCGGC ACTCAGTCTC CTCGACACCT CCAGAGTCTG 
TGTGGGATCC CAGTGGCTCA GATCAGCGCT GGAGGAAACC ACAGCTTTGT GTTGTCTCTG 
TCAGGAGTCG TGTTTGGGTG GGGGGGCAAC TCTGCTGGAC AGCTGGGTCT CGGAGACACT 
ACAGACAGAT TCGTCCCAAC AGTTGTAAAT AGTCTTAAAC GAAAGAAGAT TGTGTCCATC 
TCATGTGGAG GAGAACACAC TGCAGCTCTA GCAAAGGGAG GAACAGTGTT CACATTTGGA 
TCAGGAGGTT TCGGTCAGCT TGGGCACAAC TCATTTAAAG ATGAACATCA TCCACGACTG 
GTTGCTGAAC TGTGGGGATC TAAAGTGTCA CAGGTCACAT GTGGGAGAAA TCACACACTT 
GTATCGACTG CATCACCAAA GCTGATCTAC TCATTTGGAT GTGGGATGCA AGGGCAGCTG 
GGAAATGGAG AACAGGTCAA GCAGTCTGTG CCGTTACCAG TAGACCAGCC AACTGAATAT 
AATGACGAAT ATACAATTCA AAAACTTGTC GCGGGGGAGA ATCATTCGTT TGCCTTGTAT 
TTCAAAGAAC TTGGAAATGA GGCTAATCCA GATCCAACCA GAGGGATTAT GACATTTGAT 
AACGGAATGA TTGAACAATG CGAGTGTGGA AAAGATCCAC GGAAAGCGAT AAAGGACTTG 
AAAAAAGTGT TCTCCTCTGC TGCATCTCTA AATGGAAGTT TTCTCAAAAC AAGGCATGAT 
GGTCATTATC AGACGTCTGA AGAGTATTGT GGTTTGGATT TTGGTCTGGT TGAATCTTCC 
ATTGCAACTT TATCAAGGAA TGAAAGGCTT ATCTCAACGG TTTTGAAAGT GCTCCAAGAG 
TCACTGTTGC CAACTCTAAA TCCAAATCCA ACTGGTGAGG AATCACTGAG ACTCTATATT 
CTCCTGCCTG AGCTGATCAG CCATCTCAAG AGTGAACAAA AAGCAGAACT TACTGAAGCT 
CTGGCCTCCA AAATACTTAA TCTAAGTCCT GCTGCTCTGA GAGTGTTAGA GATCTACTGG 
TCCAAACTCC CTGATGACTG GCTTAAAAAC TTGGTTAAGT TATTCAAAAA GGCATCAGCT 
AAATTCTTTG GGAGGATCAT TGTCGAGAAC GTGAACAATG TGGCTAAAAC ACACATGCTA 
AAATTTGTGG AGATTCTTCA AAGGGTCTAT CAGGTCTGCT GCAGCGCCAA CAGAAATATC 
AAGAAGAGGT ATTTCATCAT TCATGAGGTC AACAATCTCC TAGATGTGCT ACAAACTATG 
AATGCAGATG TTAATAACAA CTGGCCTTGG TTTAACCTTA CTGGACAAAT CCATGCCCTG 
GAGGCACAAC GAAATGCGTA TGAAGAAACT TTTAGAAAGC TGACATCATT TCCATGCATC 
TTTAATCTGG AGGCCAAATG TAGCTATATG AGGAACAGAG TATGGAAGGG TCGTTTTGAG 
CTGACTGTGA GACGCACAGC ACTGCTAGAG GATTGTTTAT GTCTACTAAA CCCAGGCACC 
CAACAGCATC TCAGAGATAA ATGGCTGTCG ATTCTTTTCA CTGAGAACGT GAGCAAAAGA 
ACAGACGTCC ACAAGAGGGA CTTCTTTCTC AACGTGTTTA AGGAGCTTTG TGATCCAGCT 
TCTCAGCTCT TCATGTATAA CGACAACGAA ACTATGATCT GGTTCCCCGC GAAGCTCACC 
GTGGAGAAGA AGAAGTACTT CCTGTTTGGG ATTCTGTGTG GCTTGGCCTT CAACAACAGC 
TCTGTAGTCA ATCTGCCTTT CCCTTTAGCC CTGTTCAAGA AACTTCTCAA CATCAAACCA 
TCTCTGGAAG ATTTTATAGA GTTTAATCCA GTCCATGGCA GGAGTTTACA GTACATCCTA 
GAGTACAGTA ATGATGTTCT GGAGGAATCG TCCTTGCCCT TAACGATAAT ATGGAGTGGA 
TCAGAGCTGG ACCTGGACCC TGCTGAGCCA GAACAGCACA TCACGACTTC TAACAGAACT 
AAGTTTGTGG ATGAATATGT GGATCACATC TTCAATCAAT CAGTGAAGGA GGCGTTTGAA 
GAATTTAGAA GAGGCTTCTT TAGAGGATGT GAGAAACGCT TGGTGGAGAT GTTTGAACCA 
GAAGAACTGA GGGGGGTGCT GGTGGGCAAT GAGGAATATG ACTGGAATAT ACTCAAGCAG 
AACACTACAT ATGAAGGCGT ATTTAATGCG GATCATCCAG TCATAATCTC TTTCTGGGAG 
GTGTTTGATG ATCTGACACC AAATGAGAAG AAGTCCTTCT TGTTGTTTTT GACGGGGTTT 
GAGAGAGTGC CTATTCTGGG CATGAGCGCG GTGAAGATGA GAGTGACACA TTTGGCTAAC 
TCAACTGAGG ATCATCTGCC AGAGACTCTC ACCTGCCATT CTCTTCTGCA GCTTCCTAAA 
TATGAGAAAA GGGAAACCCT CAGAGCCAAA GTTATTGAGG CCATCAGCCA CAAGAGGGGG 
TTCTGGGAAG AATGA