sec24d cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec24d
Entrez Gene ID	553407
Full Name	SEC24 homolog D, COPII coat complex component
General protein information	Preferred Names SEC24 homolog D, COPII coat complex component Names protein transport protein Sec24D SEC24 family, member D bul bulldog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001177932.1	NP_001171403.1	protein transport protein Sec24D
XM_021477529.1	XP_021333204.1	protein transport protein Sec24D isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sec24d	XP_003749437.1
Pan troglodytes (chimpanzee)	SEC24D	XP_003310504.1
	SEC24D	XP_002804229.1
Mus musculus (house mouse)	Sec24d	NP_081411.2
Danio rerio (zebrafish)	sec24d	NP_001171403.1
Xenopus tropicalis (tropical clawed frog)	sec24d	XP_002934294.1
Homo sapiens (human)	SEC24D	NP_055637.2
Canis lupus familiaris (dog)	SEC24D	XP_005639375.1
Bos taurus (cattle)	SEC24D	XP_005207647.1
Gallus gallus (chicken)	SEC24D	XP_004941299.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Modulation of the secretory pathway rescues zebrafish polycystic kidney disease pathology.
Le Corre S, Eyre D, Drummond IA
Journal of the American Society of Nephrology : JASN25(8)1749-59(2014 Aug)

The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis.
Melville DB, Montero-Balaguer M, Levic DS, Bradley K, Smith JR, Hatzopoulos AK, Knapik EW
Disease models & mechanisms4(6)763-76(2011 Nov)

Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis.
Sarmah S, Barrallo-Gimeno A, Melville DB, Topczewski J, Solnica-Krezel L, Knapik EW
PloS one5(4)e10367(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sec24D is indispensable for chondrocyte maturation
Title: Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis.

The following sec24d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec24d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa143293	XM_021477529.1 Latest version!	Danio rerio SEC24 homolog D, COPII coat complex component (sec24d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa04571	NM_001177932.1 Latest version!	Danio rerio SEC24 homolog D, COPII coat complex component (sec24d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec24d ( XM_021477529.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333204.1 Danio rerio SEC24 homolog D, COPII coat complex component (sec24d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143293
Clone ID Related Accession (Same CDS sequence)	XM_021477529.1
Accession Version	XM_021477529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec24D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) and transcript sequence (GFIL01034707.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 175 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGTCAGC AAGGTTATGT CGCCACCCCT CCATACTCCC AGGCCCAGGC TGGAATGGGG 
GGATACGCTG CAGGTTTCGG GAACCCTCCA CCTCAGTCTC ATTATGGGGC CTATGGATCT 
CCAACACAGG GTTATTCAGC CCCTCCGACA GGTGTGCTCA AGCCTCCTGC CTCCTCTCCT 
TCTGGGATGC CTCCACCACC GGTATCCAGC CAGTATGGCA CTAACATTCA GCAGAACGGC 
GCCCATCCAC ACAGTTTCCC TCCTCCAGTT TCTTCATCTC CTTCTATGTC TCCTTATGGT 
CAGCCTCCTC CAACTGCTTT TCATAGTATG GCACAAGCCC TGCCTCCAAC TCAGCAGCTG 
ACCAATCAAA TGAGTGCCAT GAATATTGCA GGATATGGTC AAAGGCCTGT GCAGTCTCCC 
TCAAACTCAG CACCAGCACA CTTCCAAACT TCACCACCTC CACCTGTGAT GGGACATCCA 
CCGCTTTCTC CACCAGGACA GCAGCCGTCT CCTCTAGGAT CTCCTCCACC AATGGCCACA 
ATGGGATCTA TGCCAGCTCC ACCAATGGGA ATGTCTGGCC CGCCTGGACC AGTAGTCCAC 
CAGCCGCCAC ATGGACCACA GGGATATCCA CAGCAACCCG GGAGCCCGAT CGCTGGCCCG 
TATGGTGCAC AAATGGCAGG ACCACAGATG GCAGGACCAC AGACAGGCGC TTTCCCAGGA 
GGCTTTGCTG GAGGACCAGC ACAAATGGCA GGCCCTCCCC AGAAGAAGCT GGACCCAGAC 
TCTATTCCAA GCACGACACA AATGATTGCG GATGACCAGG CTAAAAGAGG AGGTCAAGTG 
TACGTCACAA ACATCAGAGG TCAGGTGCCG CCTCTGGTCA CCACAAATTT CACTGTACAG 
GATCAAGGGA ATGCCAGCCC GAGGTTTATG CGATGCACAA CATATTCATT CCCTTGCACT 
GCTGACCTTG CAAAGCAGTG CAAAGTGCCT TTGGCAGCCA TTATCAAACC CTTTGCCACC 
GTGCCCAAAA ATGAGACTCC TCTGTACGTT GTAAATCATG GAGAGACTGG ACCAATCCGC 
TGCAACCGCT GCAAGGCCTA CATGTGTCCC TACATGCAGT TTATTGACGG AGGACGACGC 
TTCCAGTGCA GCTTCTGCAG CTGCGTTAAT GAAGTTCCAG TGCATTATTT CCAGCATCTG 
GACCATATGG GGAGAAGAAT GGATCTGTAT GAGAGACCAG AACTGTCTTT GGGCTCGTAT 
GAGTTTGCCG CCACTTTGGA TTATTGCAAG AATAATAAGC CTCCTAATCC TCCTGCCTAC 
ATCTTCATGA TCGATGTCTC TTACAACAAC ATAAAAAGTG GACTTGTCAG ACTAATATGC 
GAGGAGCTGA AGACGCTGCT TGACCGACTT CCAAAGGAGG AAGGAGCAGA GAGTTCGTCC 
ATCAAGGTTG GTTTTGTGAC CTATAATAAG ATCCTGCACT TCTACAATGT GAAGAGCGCT 
CTGGCGCAGC CGCAGATGAT GGTGGTCTCC GACACAGCAG AGATGTTTGT GCCGCTTCTT 
GACGGCTTCC TCGTCAACTT CCAGGAGTCT CGAGCTGTCA TCAACAACCT TTTAGATCAG 
ATCCCTGACA TGTTTGCTGA CACCAACGAG AGCGAGACTG TTTTTGCACC CGTCGTCCAG 
GCTGGATTGG AGGCACTAAA GGCAGCTGAA TGCAGTGGCA AACTCTTCAT CTTCCACTCC 
TCCATCCCAA CAGCTGAGGC TCCAGGAAAG CTGAAAAACA GAGATGACAA AAAACTAGTG 
GGGACAGAAA AGGAGAAGAC TCTGTTCCAG CCTCAGCGGG GTGTGTATGA GCAGCTCACT 
AAAGACTGTG TGGCTCAGGG ATGTTGTGTC GACCTCTTCC TGTTCCCCAA TCAGTATGTG 
GATATCGCCA CCATGGGGGA CGTGCCCTCA CACACTGGCG GCTCAATCTA CAAGTACAGC 
AACTTCCAGG TTGAGGTAAA TGGCCAGCAG TTTCTCAGTG ACCTCAGTAG AGACGTAGAG 
AAATCCATTG GATTTGATGC CATCATGCGT GTGCGTACTG GCACAGGCTT CAGGGCGACA 
GATTTCTTCG GTGCCGTGTA CATGAACAAC ACCACAGATG TGGAGATGGC AGCGGTAGAC 
TGTGATAAGG CTGTGACGGT CGAGTTTAAA CATGACGACA CACTTAGTGA GGAGTCTGGA 
GCTGTAATGC AGTGTGCTTT GCTCTACACC ACTATTGGTG GACAGAGACG TCTGCGAATT 
CACAACCTGA GTCTGAACTG CAGCTCTCAG CTTTCTGAGC TCTACAAGAG CTGTGAGACT 
GACGCGCTCA TCAACTTCTT TGCCAAATCA GCGTATCGGG CGATGCTCAA CCAGCCGATG 
AAGACCGTGA GGGAAATCCT GGTCAACCAG ACGGCTCACA TGCTGGCCTG TTACAGGAAA 
AACTGCGCCA GTCCGTCAGC AGTCAGCCAG CTGATCCTGC CTGATGCCAT GAAGGTGTTT 
CCGGTCTACA TGAATAGTTT GCTGAAGACT CCGGCTCTAG TAAGCAGCAC TGAGCTCTCC 
ACCGACGACA GGGCCTTTCA CAGATTCATG GTCAACGCTA TGGGTGTGGA GGAAACACAG 
GTCCTGCTGT ACCCGCGCCT CATCCCGCTG CACACTATGG ATGTGTCCAG CGAGTCTCTG 
CCGGCTGCGG TGCGCTGCTC CGAGGAGAGA CTCAATGAAT CCGGGATGTT CCTGCTGGAA 
AACGGCCTCT CTATGTTCCT ATGGCTAGGA CAGGCCTGTT CACCAGACCT CATCCAGAAT 
CTCTTCAATG TGCCCTCCTT CGGTCATCTG CCCGCAGAAG GGACGTCATT ACCTGTGCTG 
GATAACCCTC ATTCCCGCAA ACTCCATTCA ATCATCTCCA GAATTTCACA GCAGAGAGCA 
GCATCCATGA AGCTGCTGAT CGTGAAGCAG AAGGATCGAG CAGAGATGTC GTTTCGTCAG 
CTGCTGGTGG AGGATAAAGG TCTTCACGGA GGAGCTTCTT ACATGGACTT TCTGTGCTTC 
GTCCACCGCG AGATCCGACA GCTGCTCACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333204.1
CDS	1..3093
Translation	MSQQGYVATPPYSQAQAGMGGYAAGFGNPPPQSHYGAYGSPTQGYSAPPTGVLKPPASSPSGMPPPPVSSQYGTNIQQNGAHPHSFPPPVSSSPSMSPYGQPPPTAFHSMAQALPPTQQLTNQMSAMNIAGYGQRPVQSPSNSAPAHFQTSPPPPVMGHPPLSPPGQQPSPLGSPPPMATMGSMPAPPMGMSGPPGPVVHQPPHGPQGYPQQPGSPIAGPYGAQMAGPQMAGPQTGAFPGGFAGGPAQMAGPPQKKLDPDSIPSTTQMIADDQAKRGGQVYVTNIRGQVPPLVTTNFTVQDQGNASPRFMRCTTYSFPCTADLAKQCKVPLAAIIKPFATVPKNETPLYVVNHGETGPIRCNRCKAYMCPYMQFIDGGRRFQCSFCSCVNEVPVHYFQHLDHMGRRMDLYERPELSLGSYEFAATLDYCKNNKPPNPPAYIFMIDVSYNNIKSGLVRLICEELKTLLDRLPKEEGAESSSIKVGFVTYNKILHFYNVKSALAQPQMMVVSDTAEMFVPLLDGFLVNFQESRAVINNLLDQIPDMFADTNESETVFAPVVQAGLEALKAAECSGKLFIFHSSIPTAEAPGKLKNRDDKKLVGTEKEKTLFQPQRGVYEQLTKDCVAQGCCVDLFLFPNQYVDIATMGDVPSHTGGSIYKYSNFQVEVNGQQFLSDLSRDVEKSIGFDAIMRVRTGTGFRATDFFGAVYMNNTTDVEMAAVDCDKAVTVEFKHDDTLSEESGAVMQCALLYTTIGGQRRLRIHNLSLNCSSQLSELYKSCETDALINFFAKSAYRAMLNQPMKTVREILVNQTAHMLACYRKNCASPSAVSQLILPDAMKVFPVYMNSLLKTPALVSSTELSTDDRAFHRFMVNAMGVEETQVLLYPRLIPLHTMDVSSESLPAAVRCSEERLNESGMFLLENGLSMFLWLGQACSPDLIQNLFNVPSFGHLPAEGTSLPVLDNPHSRKLHSIISRISQQRAASMKLLIVKQKDRAEMSFRQLLVEDKGLHGGASYMDFLCFVHREIRQLLT

Target ORF information:

RefSeq Version	XM_021477529.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC24 homolog D, COPII coat complex component (sec24d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477529.1

ORF Insert Sequence:

ATGAGTCAGC AAGGTTATGT CGCCACCCCT CCATACTCCC AGGCCCAGGC TGGAATGGGG 
GGATACGCTG CAGGTTTCGG GAACCCTCCA CCTCAGTCTC ATTATGGGGC CTATGGATCT 
CCAACACAGG GTTATTCAGC CCCTCCGACA GGTGTGCTCA AGCCTCCTGC CTCCTCTCCT 
TCTGGGATGC CTCCACCACC GGTATCCAGC CAGTATGGCA CTAACATTCA GCAGAACGGC 
GCCCATCCAC ACAGTTTCCC TCCTCCAGTT TCTTCATCTC CTTCTATGTC TCCTTATGGT 
CAGCCTCCTC CAACTGCTTT TCATAGTATG GCACAAGCCC TGCCTCCAAC TCAGCAGCTG 
ACCAATCAAA TGAGTGCCAT GAATATTGCA GGATATGGTC AAAGGCCTGT GCAGTCTCCC 
TCAAACTCAG CACCAGCACA CTTCCAAACT TCACCACCTC CACCTGTGAT GGGACATCCA 
CCGCTTTCTC CACCAGGACA GCAGCCGTCT CCTCTAGGAT CTCCTCCACC AATGGCCACA 
ATGGGATCTA TGCCAGCTCC ACCAATGGGA ATGTCTGGCC CGCCTGGACC AGTAGTCCAC 
CAGCCGCCAC ATGGACCACA GGGATATCCA CAGCAACCCG GGAGCCCGAT CGCTGGCCCG 
TATGGTGCAC AAATGGCAGG ACCACAGATG GCAGGACCAC AGACAGGCGC TTTCCCAGGA 
GGCTTTGCTG GAGGACCAGC ACAAATGGCA GGCCCTCCCC AGAAGAAGCT GGACCCAGAC 
TCTATTCCAA GCACGACACA AATGATTGCG GATGACCAGG CTAAAAGAGG AGGTCAAGTG 
TACGTCACAA ACATCAGAGG TCAGGTGCCG CCTCTGGTCA CCACAAATTT CACTGTACAG 
GATCAAGGGA ATGCCAGCCC GAGGTTTATG CGATGCACAA CATATTCATT CCCTTGCACT 
GCTGACCTTG CAAAGCAGTG CAAAGTGCCT TTGGCAGCCA TTATCAAACC CTTTGCCACC 
GTGCCCAAAA ATGAGACTCC TCTGTACGTT GTAAATCATG GAGAGACTGG ACCAATCCGC 
TGCAACCGCT GCAAGGCCTA CATGTGTCCC TACATGCAGT TTATTGACGG AGGACGACGC 
TTCCAGTGCA GCTTCTGCAG CTGCGTTAAT GAAGTTCCAG TGCATTATTT CCAGCATCTG 
GACCATATGG GGAGAAGAAT GGATCTGTAT GAGAGACCAG AACTGTCTTT GGGCTCGTAT 
GAGTTTGCCG CCACTTTGGA TTATTGCAAG AATAATAAGC CTCCTAATCC TCCTGCCTAC 
ATCTTCATGA TCGATGTCTC TTACAACAAC ATAAAAAGTG GACTTGTCAG ACTAATATGC 
GAGGAGCTGA AGACGCTGCT TGACCGACTT CCAAAGGAGG AAGGAGCAGA GAGTTCGTCC 
ATCAAGGTTG GTTTTGTGAC CTATAATAAG ATCCTGCACT TCTACAATGT GAAGAGCGCT 
CTGGCGCAGC CGCAGATGAT GGTGGTCTCC GACACAGCAG AGATGTTTGT GCCGCTTCTT 
GACGGCTTCC TCGTCAACTT CCAGGAGTCT CGAGCTGTCA TCAACAACCT TTTAGATCAG 
ATCCCTGACA TGTTTGCTGA CACCAACGAG AGCGAGACTG TTTTTGCACC CGTCGTCCAG 
GCTGGATTGG AGGCACTAAA GGCAGCTGAA TGCAGTGGCA AACTCTTCAT CTTCCACTCC 
TCCATCCCAA CAGCTGAGGC TCCAGGAAAG CTGAAAAACA GAGATGACAA AAAACTAGTG 
GGGACAGAAA AGGAGAAGAC TCTGTTCCAG CCTCAGCGGG GTGTGTATGA GCAGCTCACT 
AAAGACTGTG TGGCTCAGGG ATGTTGTGTC GACCTCTTCC TGTTCCCCAA TCAGTATGTG 
GATATCGCCA CCATGGGGGA CGTGCCCTCA CACACTGGCG GCTCAATCTA CAAGTACAGC 
AACTTCCAGG TTGAGGTAAA TGGCCAGCAG TTTCTCAGTG ACCTCAGTAG AGACGTAGAG 
AAATCCATTG GATTTGATGC CATCATGCGT GTGCGTACTG GCACAGGCTT CAGGGCGACA 
GATTTCTTCG GTGCCGTGTA CATGAACAAC ACCACAGATG TGGAGATGGC AGCGGTAGAC 
TGTGATAAGG CTGTGACGGT CGAGTTTAAA CATGACGACA CACTTAGTGA GGAGTCTGGA 
GCTGTAATGC AGTGTGCTTT GCTCTACACC ACTATTGGTG GACAGAGACG TCTGCGAATT 
CACAACCTGA GTCTGAACTG CAGCTCTCAG CTTTCTGAGC TCTACAAGAG CTGTGAGACT 
GACGCGCTCA TCAACTTCTT TGCCAAATCA GCGTATCGGG CGATGCTCAA CCAGCCGATG 
AAGACCGTGA GGGAAATCCT GGTCAACCAG ACGGCTCACA TGCTGGCCTG TTACAGGAAA 
AACTGCGCCA GTCCGTCAGC AGTCAGCCAG CTGATCCTGC CTGATGCCAT GAAGGTGTTT 
CCGGTCTACA TGAATAGTTT GCTGAAGACT CCGGCTCTAG TAAGCAGCAC TGAGCTCTCC 
ACCGACGACA GGGCCTTTCA CAGATTCATG GTCAACGCTA TGGGTGTGGA GGAAACACAG 
GTCCTGCTGT ACCCGCGCCT CATCCCGCTG CACACTATGG ATGTGTCCAG CGAGTCTCTG 
CCGGCTGCGG TGCGCTGCTC CGAGGAGAGA CTCAATGAAT CCGGGATGTT CCTGCTGGAA 
AACGGCCTCT CTATGTTCCT ATGGCTAGGA CAGGCCTGTT CACCAGACCT CATCCAGAAT 
CTCTTCAATG TGCCCTCCTT CGGTCATCTG CCCGCAGAAG GGACGTCATT ACCTGTGCTG 
GATAACCCTC ATTCCCGCAA ACTCCATTCA ATCATCTCCA GAATTTCACA GCAGAGAGCA 
GCATCCATGA AGCTGCTGAT CGTGAAGCAG AAGGATCGAG CAGAGATGTC GTTTCGTCAG 
CTGCTGGTGG AGGATAAAGG TCTTCACGGA GGAGCTTCTT ACATGGACTT TCTGTGCTTC 
GTCCACCGCG AGATCCGACA GCTGCTCACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec24d ( NM_001177932.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001171403.1 Danio rerio SEC24 homolog D, COPII coat complex component (sec24d), mRNA.

Price & Availability↑

CloneID	ODa04571
Clone ID Related Accession (Same CDS sequence)	NM_001177932.1
Accession Version	NM_001177932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec24D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU944484.1. On May 6, 2010 this sequence version replaced XM_002667412.1. ##Evidence-Data-START## Transcript exon combination :: GU944484.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTCAGC AAGGTTATGT CGCCACCCCT CCATACTCCC AGGCCCAAGC TGGAATGGGG 
GGATACGCTG CAGGTTTCGG GAACCCTCCA CCTCAGTCTC ATTATGGGGC CTATGGATCT 
CCAACACAGG GTTATTCAGC CCCTCCGCCA GGTGTGCTCA AGCCTCCTGC CTCCTCTCCT 
TCTGGGATGC CTCCACCACC GGTATCCAGC CAGTATGGCA CTAACATTCA GCAGAACGGC 
GCCCATCCAC ACAGTTTCCC TCCTCCAGTT TCTTCATCTC CTTCTATGTC TCCTTATGGT 
CAGCCTCCTC CAACTGCTTT TCGTAGTATG GCACAAGCTC CGCCTCCAAC TCAGCAGCTG 
ACCAATCAAA TGAGTGCCAT GAATATTGCA GGATATGGTC AAAGGCCTGT GCAGTCTCCC 
TCAAACTCAG CACCAGCACA CTTCCAAACT TCACCACCTC CACCTGTGAT GGGACATCCA 
CCGCTTTCTC CACCAGGACA GCAGCCGTCT CCTCTAGGAT CTCCTCCACC AATGGCCACA 
ATGGGATCTA TGCCAGCTCC ACCAATGGGA ATGTCTGGCC CGCCTGGACC AGTAGTCCAC 
CAGCCGCCAC ATGGACCACA GGGATATCCA CAGCAACCCG GGAGCCCGAT CGCTGGCCCG 
TATGGTGCAC AAATGGCAGG ACCACAGGGG CAAGGACCAC AGACAGGCGC TTTCCCAGGA 
GGCTTTGCTG GAGGACCAGC ACAAATGGCA GGCCCTCCTC AGAAGAAGCT TGACCCAGAC 
TCTATTCCAA GCACGACACA AATGATGCGG ATGACCAGGC TAAAGAGGAG GCAGGTGTAC 
GTCACAAACA CAAGAGGTCA GGTGCCGCCT CTGGTCACCT CAAAATTCAC TGTACAGGAT 
CAAGGGAATG CCAGCCCGAG GTTTATGCGA TGCACAACAT ATTCATTCCC TTGCACTGCT 
GACCTTGCAA AGCAGTGCAA AGTGCCTTTG GCAGCCATTA TCAAACCCTT CGCCGCCGTG 
CCCAAAAATG AGACTCCTCT GTACGTTGTA AATCATGGAG AGACTGGACC AATCCGCTGC 
AACCGCTGCA AGGCCTACAT GTGTCCCTAC ATGCAGTTTA TTGACGGAGG ACGACGATTC 
CAGTGCAGCT TCTGCAGCTG TGTTAATGAA GTTCCAGTGC ATTATTTCCA GCATCTGGAC 
CATATGGGGA GAAGAATGGA TCTGTATGAG AGACCAGAAC TGTCTTTGGG CTCGTATGAG 
TTTGCCGCCA CTTTGGATTA TTGCAAGAAT AATAAGCCTC CTAATCCTCC TGCCTACATC 
TTCATGATCG ATGTCTCTTA CAACAACATA AAAAGTGGAC TTGTCAGACT AATATGCGAG 
GAGCTGAAGA CGCTGCTTGA CCGACTTCCA AAGGAGGAAG GAGCAGAGAG TTCGTCCATC 
AAGGTTGGTT TTGTGACCTA TAATAAGATC CTGCACTTCT ACAATGTGAA GAGTGCTCTG 
GCGCAGCCGC AGATGATGGT GGTCTCCGAC ACAGCAGAGA TGTTTGTGCC GCTTCTTGAC 
GGCTTCCTCG TCAACTTCCA GGAGTCTCGA GCTGTCATCA ACAACCTTTT AGATCAGATC 
CCTGACATGT TTGCTGACAC CAACGAGAGC GAGACTGTTT TTGCACCCGT CATCCAGGCT 
GGATTGGAGG CACTAAAGGC AGCTGAATGC AGTGGCAAAC TCTTCATCTT CCACTCCTCC 
ATCCCAACAG CTGAGGCTCC AGGAAAGCTG AAAAACAGAG ATGACAAAAA ACTAGTGGGG 
ACAGAAAAGG AGAAGACTCT GTTCCAGCCT CAGCGGGGTG TGTATGAGCA GCTCACTAAA 
GACTGTGTGG CTCAGGGATG TTGTGTCGAC CTCTTCCTGT TCCCCAATCA GTATGTGGAT 
ATCGCCACCA TGGGGGACGT GCCCTCACAC ACTGGCGGCT CAATCTACAA GTACAGCAAC 
TTCCAGGTTG AGGTAAATGG CCAGCAGTTT CTCAGTGACC TCAGTAGAGA CGTAGAGAAA 
TCCATTGGAT TTGATGCCAT CATGCGTGTG CGTACTGGCA CAGGCTTCAG GGCGACAGAT 
TTCTTCGGTG CCGTGTACAT GAACAACACC ACAGATGTGG AGATGGCAGC GGTAGACTGT 
GATAAGGCTG TGACGGTCGA GTTTAAACAT GACGACACAC TTAGTGAGGA GTCTGGAGCT 
GTAATGCAGT GTGCTTTGCT CTACACCACT ATTGGTGGAC AGAGACGTCT GCGAATTCAC 
AACCTGAGTC TGAACTGCAG CTCTCAGCTT TCTGAGCTCT ACAAGAGCTG TGAGACTGAC 
GCGCTCATCA ACTTCTTTGC CAAATCAGCG TATCGGGCGA TGCTCAACCA GCCGATGAAG 
ACCGTGAGGG AAATCCTGGT CAACCAGACG GCTCACATGC TGGCCTGTTA CAGGAAAAAC 
TGCGCCAGTC CGTCAGCAGT CAGCCAGCTG ATCCTGCCTG ATGCCATGAA GGTGTTTCCG 
GTCTACATGA ACAGTTTGCT GAAGACTCCG GCTCTAGTAA GCAGCACTGA GCTCTCCACC 
GACGACAGGG CCTTTCACAG ATTCATGGTC AACGCTATGG GTGTGGAGGA AACACAGGTC 
CTGCTGTACC CGCGCCTCAT CCCGCTGCAC ACTATGGATG TGTCCAGCGA GTCTCTGCCG 
GCTGCGGTGC GCTGCTCCGA GGAGAGACTC AATGAATCCG GGATGTTCCT GCTGGAAAAC 
GGCCTCTCTA TGTTCCTATG GCTAGGACAG GCCTGTTCAC CAGACCTCAT CCAGAATCTC 
TTCAATGTGC CCTCCTTCGG TCATCTGCCC GCAGAAGGGA CGTCATTACC TGTGCTGGAT 
AACGCTCATT CCCGCAAACT CCATTCAATC ATCTCCAGAA TTTCACAACA GAGAGCAGCA 
TCCATGAAGC TGCTGATCGT GAAGCAGAAG GATCGAGCAG AGATGTCGAT TCGTCAGCTG 
CTGGTGGAGG ATAAAGGTCT TCACGGAGGA GCTTCTTACA TGGACTTTCT GTGCTTCGTC 
CACCGCGAGA TCCGACAGCT GCTCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171403.1
CDS	121..3210
Translation	MSQQGYVATPPYSQAQAGMGGYAAGFGNPPPQSHYGAYGSPTQGYSAPPPGVLKPPASSPSGMPPPPVSSQYGTNIQQNGAHPHSFPPPVSSSPSMSPYGQPPPTAFRSMAQAPPPTQQLTNQMSAMNIAGYGQRPVQSPSNSAPAHFQTSPPPPVMGHPPLSPPGQQPSPLGSPPPMATMGSMPAPPMGMSGPPGPVVHQPPHGPQGYPQQPGSPIAGPYGAQMAGPQGQGPQTGAFPGGFAGGPAQMAGPPQKKLDPDSIPSTTQMMRMTRLKRRQVYVTNTRGQVPPLVTSKFTVQDQGNASPRFMRCTTYSFPCTADLAKQCKVPLAAIIKPFAAVPKNETPLYVVNHGETGPIRCNRCKAYMCPYMQFIDGGRRFQCSFCSCVNEVPVHYFQHLDHMGRRMDLYERPELSLGSYEFAATLDYCKNNKPPNPPAYIFMIDVSYNNIKSGLVRLICEELKTLLDRLPKEEGAESSSIKVGFVTYNKILHFYNVKSALAQPQMMVVSDTAEMFVPLLDGFLVNFQESRAVINNLLDQIPDMFADTNESETVFAPVIQAGLEALKAAECSGKLFIFHSSIPTAEAPGKLKNRDDKKLVGTEKEKTLFQPQRGVYEQLTKDCVAQGCCVDLFLFPNQYVDIATMGDVPSHTGGSIYKYSNFQVEVNGQQFLSDLSRDVEKSIGFDAIMRVRTGTGFRATDFFGAVYMNNTTDVEMAAVDCDKAVTVEFKHDDTLSEESGAVMQCALLYTTIGGQRRLRIHNLSLNCSSQLSELYKSCETDALINFFAKSAYRAMLNQPMKTVREILVNQTAHMLACYRKNCASPSAVSQLILPDAMKVFPVYMNSLLKTPALVSSTELSTDDRAFHRFMVNAMGVEETQVLLYPRLIPLHTMDVSSESLPAAVRCSEERLNESGMFLLENGLSMFLWLGQACSPDLIQNLFNVPSFGHLPAEGTSLPVLDNAHSRKLHSIISRISQQRAASMKLLIVKQKDRAEMSIRQLLVEDKGLHGGASYMDFLCFVHREIRQLLT

Target ORF information:

RefSeq Version	NM_001177932.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC24 homolog D, COPII coat complex component (sec24d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177932.1

ORF Insert Sequence:

ATGAGTCAGC AAGGTTATGT CGCCACCCCT CCATACTCCC AGGCCCAAGC TGGAATGGGG 
GGATACGCTG CAGGTTTCGG GAACCCTCCA CCTCAGTCTC ATTATGGGGC CTATGGATCT 
CCAACACAGG GTTATTCAGC CCCTCCGCCA GGTGTGCTCA AGCCTCCTGC CTCCTCTCCT 
TCTGGGATGC CTCCACCACC GGTATCCAGC CAGTATGGCA CTAACATTCA GCAGAACGGC 
GCCCATCCAC ACAGTTTCCC TCCTCCAGTT TCTTCATCTC CTTCTATGTC TCCTTATGGT 
CAGCCTCCTC CAACTGCTTT TCGTAGTATG GCACAAGCTC CGCCTCCAAC TCAGCAGCTG 
ACCAATCAAA TGAGTGCCAT GAATATTGCA GGATATGGTC AAAGGCCTGT GCAGTCTCCC 
TCAAACTCAG CACCAGCACA CTTCCAAACT TCACCACCTC CACCTGTGAT GGGACATCCA 
CCGCTTTCTC CACCAGGACA GCAGCCGTCT CCTCTAGGAT CTCCTCCACC AATGGCCACA 
ATGGGATCTA TGCCAGCTCC ACCAATGGGA ATGTCTGGCC CGCCTGGACC AGTAGTCCAC 
CAGCCGCCAC ATGGACCACA GGGATATCCA CAGCAACCCG GGAGCCCGAT CGCTGGCCCG 
TATGGTGCAC AAATGGCAGG ACCACAGGGG CAAGGACCAC AGACAGGCGC TTTCCCAGGA 
GGCTTTGCTG GAGGACCAGC ACAAATGGCA GGCCCTCCTC AGAAGAAGCT TGACCCAGAC 
TCTATTCCAA GCACGACACA AATGATGCGG ATGACCAGGC TAAAGAGGAG GCAGGTGTAC 
GTCACAAACA CAAGAGGTCA GGTGCCGCCT CTGGTCACCT CAAAATTCAC TGTACAGGAT 
CAAGGGAATG CCAGCCCGAG GTTTATGCGA TGCACAACAT ATTCATTCCC TTGCACTGCT 
GACCTTGCAA AGCAGTGCAA AGTGCCTTTG GCAGCCATTA TCAAACCCTT CGCCGCCGTG 
CCCAAAAATG AGACTCCTCT GTACGTTGTA AATCATGGAG AGACTGGACC AATCCGCTGC 
AACCGCTGCA AGGCCTACAT GTGTCCCTAC ATGCAGTTTA TTGACGGAGG ACGACGATTC 
CAGTGCAGCT TCTGCAGCTG TGTTAATGAA GTTCCAGTGC ATTATTTCCA GCATCTGGAC 
CATATGGGGA GAAGAATGGA TCTGTATGAG AGACCAGAAC TGTCTTTGGG CTCGTATGAG 
TTTGCCGCCA CTTTGGATTA TTGCAAGAAT AATAAGCCTC CTAATCCTCC TGCCTACATC 
TTCATGATCG ATGTCTCTTA CAACAACATA AAAAGTGGAC TTGTCAGACT AATATGCGAG 
GAGCTGAAGA CGCTGCTTGA CCGACTTCCA AAGGAGGAAG GAGCAGAGAG TTCGTCCATC 
AAGGTTGGTT TTGTGACCTA TAATAAGATC CTGCACTTCT ACAATGTGAA GAGTGCTCTG 
GCGCAGCCGC AGATGATGGT GGTCTCCGAC ACAGCAGAGA TGTTTGTGCC GCTTCTTGAC 
GGCTTCCTCG TCAACTTCCA GGAGTCTCGA GCTGTCATCA ACAACCTTTT AGATCAGATC 
CCTGACATGT TTGCTGACAC CAACGAGAGC GAGACTGTTT TTGCACCCGT CATCCAGGCT 
GGATTGGAGG CACTAAAGGC AGCTGAATGC AGTGGCAAAC TCTTCATCTT CCACTCCTCC 
ATCCCAACAG CTGAGGCTCC AGGAAAGCTG AAAAACAGAG ATGACAAAAA ACTAGTGGGG 
ACAGAAAAGG AGAAGACTCT GTTCCAGCCT CAGCGGGGTG TGTATGAGCA GCTCACTAAA 
GACTGTGTGG CTCAGGGATG TTGTGTCGAC CTCTTCCTGT TCCCCAATCA GTATGTGGAT 
ATCGCCACCA TGGGGGACGT GCCCTCACAC ACTGGCGGCT CAATCTACAA GTACAGCAAC 
TTCCAGGTTG AGGTAAATGG CCAGCAGTTT CTCAGTGACC TCAGTAGAGA CGTAGAGAAA 
TCCATTGGAT TTGATGCCAT CATGCGTGTG CGTACTGGCA CAGGCTTCAG GGCGACAGAT 
TTCTTCGGTG CCGTGTACAT GAACAACACC ACAGATGTGG AGATGGCAGC GGTAGACTGT 
GATAAGGCTG TGACGGTCGA GTTTAAACAT GACGACACAC TTAGTGAGGA GTCTGGAGCT 
GTAATGCAGT GTGCTTTGCT CTACACCACT ATTGGTGGAC AGAGACGTCT GCGAATTCAC 
AACCTGAGTC TGAACTGCAG CTCTCAGCTT TCTGAGCTCT ACAAGAGCTG TGAGACTGAC 
GCGCTCATCA ACTTCTTTGC CAAATCAGCG TATCGGGCGA TGCTCAACCA GCCGATGAAG 
ACCGTGAGGG AAATCCTGGT CAACCAGACG GCTCACATGC TGGCCTGTTA CAGGAAAAAC 
TGCGCCAGTC CGTCAGCAGT CAGCCAGCTG ATCCTGCCTG ATGCCATGAA GGTGTTTCCG 
GTCTACATGA ACAGTTTGCT GAAGACTCCG GCTCTAGTAA GCAGCACTGA GCTCTCCACC 
GACGACAGGG CCTTTCACAG ATTCATGGTC AACGCTATGG GTGTGGAGGA AACACAGGTC 
CTGCTGTACC CGCGCCTCAT CCCGCTGCAC ACTATGGATG TGTCCAGCGA GTCTCTGCCG 
GCTGCGGTGC GCTGCTCCGA GGAGAGACTC AATGAATCCG GGATGTTCCT GCTGGAAAAC 
GGCCTCTCTA TGTTCCTATG GCTAGGACAG GCCTGTTCAC CAGACCTCAT CCAGAATCTC 
TTCAATGTGC CCTCCTTCGG TCATCTGCCC GCAGAAGGGA CGTCATTACC TGTGCTGGAT 
AACGCTCATT CCCGCAAACT CCATTCAATC ATCTCCAGAA TTTCACAACA GAGAGCAGCA 
TCCATGAAGC TGCTGATCGT GAAGCAGAAG GATCGAGCAG AGATGTCGAT TCGTCAGCTG 
CTGGTGGAGG ATAAAGGTCT TCACGGAGGA GCTTCTTACA TGGACTTTCT GTGCTTCGTC 
CACCGCGAGA TCCGACAGCT GCTCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Modulation of the secretory pathway rescues zebrafish polycystic kidney disease pathology.
Journal of the American Society of Nephrology : JASN25(8)1749-59(2014 Aug)
Le Corre S,Eyre D,Drummond IA

The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis.
Disease models & mechanisms4(6)763-76(2011 Nov)
Melville DB,Montero-Balaguer M,Levic DS,Bradley K,Smith JR,Hatzopoulos AK,Knapik EW

Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis.
PloS one5(4)e10367(2010 Apr)
Sarmah S,Barrallo-Gimeno A,Melville DB,Topczewski J,Solnica-Krezel L,Knapik EW