gtf2h1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gtf2h1
Entrez Gene ID	447857
Full Name	general transcription factor IIH, polypeptide 1
Synonyms	zgc:92126
General protein information	Preferred Names general transcription factor IIH, polypeptide 1 Names general transcription factor IIH subunit 1 p62 zmp:0000000569
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004596.1	NP_001004596.1	general transcription factor IIH subunit 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GTF2H1	XP_001173120.1
Bos taurus (cattle)	GTF2H1	NP_001039615.1
Rattus norvegicus (Norway rat)	Gtf2h1	NP_001101955.1
Gallus gallus (chicken)	GTF2H1	NP_001186521.1
Oryza sativa (rice)	Os08g0338200	NP_001061583.1
Mus musculus (house mouse)	Gtf2h1	NP_032212.3
Danio rerio (zebrafish)	gtf2h1	NP_001004596.1
Arabidopsis thaliana (thale cress)	AT1G55750	NP_175971.3
Xenopus tropicalis (tropical clawed frog)	gtf2h1	NP_001006921.1
Canis lupus familiaris (dog)	GTF2H1	XP_534083.1
Drosophila melanogaster (fruit fly)	Tfb1	NP_725382.1
Arabidopsis thaliana (thale cress)	AT3G61420	NP_191701.4
Homo sapiens (human)	GTF2H1	NP_001135779.1
Danio rerio (zebrafish)	LOC100536263	XP_003201424.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011110	XP_309535.4
Caenorhabditis elegans (roundworm)	CELE_R02D3.3	NP_499880.3

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Gene duplication followed by exon structure divergence substitutes for alternative splicing in zebrafish.
Lambert MJ, Olsen KG, Cooper CD
Gene546(2)271-6(2014 Aug)

Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Silva IA, Cox CJ, Leite RB, Cancela ML, Concei??o N
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology172-1739-20(2014 Jun-Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gtf2h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gtf2h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35438	NM_001004596.1 Latest version!	Danio rerio general transcription factor IIH, polypeptide 1 (gtf2h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gtf2h1 ( NM_001004596.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004596.1 Danio rerio general transcription factor IIH, polypeptide 1 (gtf2h1), mRNA.

Price & Availability↑

CloneID	ODa35438
Clone ID Related Accession (Same CDS sequence)	NM_001004596.1
Accession Version	NM_001004596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	general transcription factor IIH subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081602.1. On Jul 6, 2005 this sequence version replaced XM_690859.1. ##Evidence-Data-START## Transcript exon combination :: BC081602.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCTC TGTCCGAGGA GGTGTTGTTG GTGGTGAAGA GGGTACGTCA GAGGAAACAG 
GACGGGACAC TGTACCTTAT GGCTGAGAGG ATCGCCTGGG GACCTGAGGG AAAAGACCGC 
TTCACTGTCA GCCACCTCTA CGCCGACATC CGCTGCCAGA AGATCAGTCC TGACGGTAAA 
GCCAAGATTC AGCTTCAGCT GGTTCTGCAC ACAGGAGAAA ACACCACTTT TCACTTCGCC 
AATGAGAGCA CAGCGCTGAA GGATAGAGAA GCCGCCAAAG AGCTTCTCCA GCAGCTCCTG 
CCCAAGTTCA AGAAGAAGGC CAATAAAGAG CTGGAGGAGA AGAACAGGAT GTTGCAGGAA 
GACCCCGTCC TGTTCCAGCT CTATAAGGAC CTGGTGGTGA GTCAGGTGAT CAGCGCTGAG 
GAGTTCTGGG CCAATCGGAT GAGCCTCAAT TCAGTGGATC ACTCGCCCTC CAGTAATAAC 
GTACAGGAAG TGGGCATCTC TGCTGCCTTC CTGGCCGACA TCAGACCACA GACAGACGGC 
TGTAATGGCC TGCGGTACAA TCTGACCTCT GACATCATCG AGTCGATCTT CAGGACTTAC 
CCTACAGTCA AGCAGAAATA CGCAGAGAAC GTTCCTCACA ACATGACGGA GAAGGAGTTC 
TGGACACGTT TCTTTCAGTC TCATTATTTC CACAGAGATC GCATCAACAC GGGCCAGCAG 
GACATCTTCT CTGAGTGTGC CAAACAGGAC GAGAAGGGCC TGAAGTCTAT GGTGACCCAG 
GGAGTGAAGA ACCCAATGGT GGATCTGCTT GCACTCGAAG ATAAAACGTT AGATGAGGGT 
TACGGCACAG CGGCGGCTCC CCCTGCGTCA AACACAGCCA ACAAAACACT CAGAGAAAAC 
AGCAACTCCG CCATCATCAA GCGCTTCAAC CACCACAGTG CCATGGTCCT GGCAGCCGGC 
CTGCGGAAAG CGGAAGCCCC CAGTGATCAA GCCAGCGAGA CGAGCAGCAC GGATGGAAAC 
TCCAGAGATT CAGATTTCTT TCAGCCGCCA CTTAAAAAGG TGAAATTACA GGAAGCCATA 
GAGTATGATG ACCTGCAAAA CGACTCGGGC CGAAAGACCA TCGCTCTGAA CCTGAAGAAA 
TCAGACAGAT ATGCCCACGG GCCGGTCCCT CTTCAGTCGC AGCATTACAG CACCAGTCAG 
GACATCATCA ACTCCATCAG CATTATCCAG CACGAGATGA GGAGCTACAA ACCCCAACTC 
ACGCAGGTGA TGTCCAGCAG TGCGGCGAGC TCAGCCATCA GCGCTCTTTC ACCTGGAGGA 
GTTTTAATGC AGAGCGCAGG CCAGCAGACC ATCCACCAGT TAGTGCCCGC TGATGTTCAG 
AGCGAGCTGA AGCACTTATA CACGGCCGCC GGAGAGCTGC TCCGACACTT CTGGTCCTGT 
TTCCCCGTCA ACACTCCCTT TCTGGAGGAG AAGGTGTTGA AAATGAAATC CAACCTGGAG 
AGGTTTCAGG TGACCAAGCT CCGCCCCTTT CAAGAGAAGA TCCAGCGACA GTATTTGAGC 
ACTAATCTTA CAGGCCACCT GGAGGAGATG TTTCAGGCTG CATACAGCAA ATTTCAAGCC 
TGGCAGACAC GCCGAATGAT GAGGAAAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004596.1
CDS	144..1796
Translation	MASLSEEVLLVVKRVRQRKQDGTLYLMAERIAWGPEGKDRFTVSHLYADIRCQKISPDGKAKIQLQLVLHTGENTTFHFANESTALKDREAAKELLQQLLPKFKKKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRMSLNSVDHSPSSNNVQEVGISAAFLADIRPQTDGCNGLRYNLTSDIIESIFRTYPTVKQKYAENVPHNMTEKEFWTRFFQSHYFHRDRINTGQQDIFSECAKQDEKGLKSMVTQGVKNPMVDLLALEDKTLDEGYGTAAAPPASNTANKTLRENSNSAIIKRFNHHSAMVLAAGLRKAEAPSDQASETSSTDGNSRDSDFFQPPLKKVKLQEAIEYDDLQNDSGRKTIALNLKKSDRYAHGPVPLQSQHYSTSQDIINSISIIQHEMRSYKPQLTQVMSSSAASSAISALSPGGVLMQSAGQQTIHQLVPADVQSELKHLYTAAGELLRHFWSCFPVNTPFLEEKVLKMKSNLERFQVTKLRPFQEKIQRQYLSTNLTGHLEEMFQAAYSKFQAWQTRRMMRKT

Target ORF information:

RefSeq Version	NM_001004596.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio general transcription factor IIH, polypeptide 1 (gtf2h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004596.1

ORF Insert Sequence:

ATGGCGTCTC TGTCCGAGGA GGTGTTGTTG GTGGTGAAGA GGGTACGTCA GAGGAAACAG 
GACGGGACAC TGTACCTTAT GGCTGAGAGG ATCGCCTGGG GACCTGAGGG AAAAGACCGC 
TTCACTGTCA GCCACCTCTA CGCCGACATC CGCTGCCAGA AGATCAGTCC TGACGGTAAA 
GCCAAGATTC AGCTTCAGCT GGTTCTGCAC ACAGGAGAAA ACACCACTTT TCACTTCGCC 
AATGAGAGCA CAGCGCTGAA GGATAGAGAA GCCGCCAAAG AGCTTCTCCA GCAGCTCCTG 
CCCAAGTTCA AGAAGAAGGC CAATAAAGAG CTGGAGGAGA AGAACAGGAT GTTGCAGGAA 
GACCCCGTCC TGTTCCAGCT CTATAAGGAC CTGGTGGTGA GTCAGGTGAT CAGCGCTGAG 
GAGTTCTGGG CCAATCGGAT GAGCCTCAAT TCAGTGGATC ACTCGCCCTC CAGTAATAAC 
GTACAGGAAG TGGGCATCTC TGCTGCCTTC CTGGCCGACA TCAGACCACA GACAGACGGC 
TGTAATGGCC TGCGGTACAA TCTGACCTCT GACATCATCG AGTCGATCTT CAGGACTTAC 
CCTACAGTCA AGCAGAAATA CGCAGAGAAC GTTCCTCACA ACATGACGGA GAAGGAGTTC 
TGGACACGTT TCTTTCAGTC TCATTATTTC CACAGAGATC GCATCAACAC GGGCCAGCAG 
GACATCTTCT CTGAGTGTGC CAAACAGGAC GAGAAGGGCC TGAAGTCTAT GGTGACCCAG 
GGAGTGAAGA ACCCAATGGT GGATCTGCTT GCACTCGAAG ATAAAACGTT AGATGAGGGT 
TACGGCACAG CGGCGGCTCC CCCTGCGTCA AACACAGCCA ACAAAACACT CAGAGAAAAC 
AGCAACTCCG CCATCATCAA GCGCTTCAAC CACCACAGTG CCATGGTCCT GGCAGCCGGC 
CTGCGGAAAG CGGAAGCCCC CAGTGATCAA GCCAGCGAGA CGAGCAGCAC GGATGGAAAC 
TCCAGAGATT CAGATTTCTT TCAGCCGCCA CTTAAAAAGG TGAAATTACA GGAAGCCATA 
GAGTATGATG ACCTGCAAAA CGACTCGGGC CGAAAGACCA TCGCTCTGAA CCTGAAGAAA 
TCAGACAGAT ATGCCCACGG GCCGGTCCCT CTTCAGTCGC AGCATTACAG CACCAGTCAG 
GACATCATCA ACTCCATCAG CATTATCCAG CACGAGATGA GGAGCTACAA ACCCCAACTC 
ACGCAGGTGA TGTCCAGCAG TGCGGCGAGC TCAGCCATCA GCGCTCTTTC ACCTGGAGGA 
GTTTTAATGC AGAGCGCAGG CCAGCAGACC ATCCACCAGT TAGTGCCCGC TGATGTTCAG 
AGCGAGCTGA AGCACTTATA CACGGCCGCC GGAGAGCTGC TCCGACACTT CTGGTCCTGT 
TTCCCCGTCA ACACTCCCTT TCTGGAGGAG AAGGTGTTGA AAATGAAATC CAACCTGGAG 
AGGTTTCAGG TGACCAAGCT CCGCCCCTTT CAAGAGAAGA TCCAGCGACA GTATTTGAGC 
ACTAATCTTA CAGGCCACCT GGAGGAGATG TTTCAGGCTG CATACAGCAA ATTTCAAGCC 
TGGCAGACAC GCCGAATGAT GAGGAAAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Gene duplication followed by exon structure divergence substitutes for alternative splicing in zebrafish.
Gene546(2)271-6(2014 Aug)
Lambert MJ,Olsen KG,Cooper CD

Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology172-1739-20(2014 Jun-Jul)
Silva IA,Cox CJ,Leite RB,Cancela ML,Concei??o N