Gtf2h1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtf2h1
Entrez Gene ID	14884
Full Name	general transcription factor II H, polypeptide 1
Synonyms	62kDa,AW743425,AW822074,BTF2 p62,C77871,p62
General protein information	Preferred Names general transcription factor II H, polypeptide 1 Names general transcription factor IIH subunit 1 BTF2-p62 TFIIH basal transcription factor complex p62 subunit basic transcription factor 2 62 kDa subunit general transcription factor IIH, polypeptide 1 (62kD subunit)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540636.3	XP_006540699.1	general transcription factor IIH subunit 1 isoform X1
NM_001360075.1	NP_001347004.1	general transcription factor IIH subunit 1 isoform 3
NM_008186.4	NP_032212.3	general transcription factor IIH subunit 1 isoform 2
NM_001360076.1	NP_001347005.1	general transcription factor IIH subunit 1 isoform 1
NM_001291075.1	NP_001278004.1	general transcription factor IIH subunit 1 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GTF2H1	XP_001173120.1
Bos taurus (cattle)	GTF2H1	NP_001039615.1
Rattus norvegicus (Norway rat)	Gtf2h1	NP_001101955.1
Gallus gallus (chicken)	GTF2H1	NP_001186521.1
Oryza sativa (rice)	Os08g0338200	NP_001061583.1
Mus musculus (house mouse)	Gtf2h1	NP_032212.3
Danio rerio (zebrafish)	gtf2h1	NP_001004596.1
Arabidopsis thaliana (thale cress)	AT1G55750	NP_175971.3
Xenopus tropicalis (tropical clawed frog)	gtf2h1	NP_001006921.1
Canis lupus familiaris (dog)	GTF2H1	XP_534083.1
Drosophila melanogaster (fruit fly)	Tfb1	NP_725382.1
Arabidopsis thaliana (thale cress)	AT3G61420	NP_191701.4
Homo sapiens (human)	GTF2H1	NP_001135779.1
Danio rerio (zebrafish)	LOC100536263	XP_003201424.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011110	XP_309535.4
Caenorhabditis elegans (roundworm)	CELE_R02D3.3	NP_499880.3

Gene Ontology
Bibliography

Related articles in PubMed

The insulin-like growth factor 2 (IGF2) mRNA-binding protein p62/IGF2BP2-2 as a promoter of NAFLD and HCC?
Simon Y, Kessler SM, Bohle RM, Haybaeck J, Kiemer AK
Gut63(5)861-3(2014 May)

p62 expression and autophagy in ?B-crystallin R120G mutant knock-in mouse model of hereditary cataract.
Wignes JA, Goldman JW, Weihl CC, Bartley MG, Andley UP
Experimental eye research115263-73(2013 Oct)

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Kamileri I, Karakasilioti I, Sideri A, Kosteas T, Tatarakis A, Talianidis I, Garinis GA
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)

Autophagy and p62 in cardiac proteinopathy.
Zheng Q, Su H, Ranek MJ, Wang X
Circulation research109(3)296-308(2011 Jul)

Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation.
Linares JF, Amanchy R, Greis K, Diaz-Meco MT, Moscat J
Molecular and cellular biology31(1)105-17(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mice with a liver overexpression of insulin-like growth factor 2 (IGF2) mRNA-binding protein p62/IMP2-2/IGF2BP2-2 show elevated Ccl2 levels, which models all stages of non-alcoholic fatty liver disease.
Title: The insulin-like growth factor 2 (IGF2) mRNA-binding protein p62/IGF2BP2-2 as a promoter of NAFLD and HCC?

We propose that autophagy is inhibited in the alphaB-R120G mutant lenses because of a defect in protein degradation after autophagosome formation.
Title: p62 expression and autophagy in ?B-crystallin R120G mutant knock-in mouse model of hereditary cataract.

p62 is up-regulated in mouse proteinopathic hearts and promotes aggresome formation and autophagy activation and protects cardiomyocytes against proteotoxic stress
Title: Autophagy and p62 in cardiac proteinopathy.

cdk1 phosphorylates p62 in vitro and in vivo at T269 and S272, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis.
Title: Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation.

p62 is a crucial regulator of ERK1 in metabolism
Title: A functional role for the p62-ERK1 axis in the control of energy homeostasis and adipogenesis.

The following Gtf2h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtf2h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14426	NM_008186.4 Latest version!	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu14443	NM_001360076.1 Latest version!	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu14443	NM_001291075.1 Latest version!	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu14443	XM_006540636.3 Latest version!	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu84036	NM_001360075.1 Latest version!	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtf2h1 ( NM_008186.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032212.3 Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14426
Clone ID Related Accession (Same CDS sequence)	NM_008186.4
Accession Version	NM_008186.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	general transcription factor IIH subunit 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB610125.1, AK005065.1, BC052837.1 and BX632789.1. On May 14, 2008 this sequence version replaced NM_008186.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK005065.1, SRR1660811.131649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACTT CATCAGAAGA AGTGTTGCTG ATTGTGAAGA AGGTTCGTCA GAAGAAGCAG 
GATGGAGCAC TGTACCTCAT GGCAGAGAGA ATTGCTTGGG CACCTGAAGG CAAAGACAGA 
TTTACAATCA GCCATATGTA TGCAGATATT AAATGCCAGA AAATCAGTCC AGAGGGAAAA 
GCTAAAATAC AACTTCAGCT GGTCCTGCAT GCAGGGGACA CAACAAACTT CCATTTTTCC 
AACGAAAGCA CGGCAGTGAA AGAACGGGAT GCAGTGAAGG ACCTCCTTCA GCAGCTGCTG 
CCCAAGTTCA AGCGGAAAGC TAATAAAGAG CTGGAGGAGA AGAACAGAAT GCTCCAAGAA 
GATCCTGTTT TATTCCAACT CTATAAAGAC CTTGTTGTGA GCCAAGTGAT CAGTGCTGAG 
GAATTCTGGG CCAATCGTTT AAATGTGAAT GCAACAGATA GTTCTACATC CAGTCACAAG 
CAGGATGTTG GTATTTCTGC AGCATTTCTG GCTGATGTCC GGCCCCAAAC GGATGGCTGT 
AATGGTCTGA GATATAATTT AACTTCTGAT ATCATTGAAT CTATATTTAG GACCTATCCA 
GCAGTAAAAA TGAAATATGC AGAAACTGTA CCACACAACA TGACAGAAAA GGAGTTCTGG 
ACACGCTTTT TCCAGTCCCA TTATTTTCAC AGGGACCGAC TAAATACAGG ATCGAAGGAC 
CTCTTTGCAG AATGTGCCAA AATAGATGAG AAGGGATTAA AAACAATGGT TTCATTAGGA 
GTGAAAAACC CAATGCTAGA TTTGACATCG TTGGAAGATA AGCCATTAGA CGAGGGCTAT 
GGCATTTCCT CTGTGCCATC TACTTCCAAT TCCAAATCCA TAAAGGAGAA TAGTAATGCT 
GCCATCATCA AAAGGTTTAA CCACCACAGT GCCATGGTCC TGGCTGCTGG TCTCAGGAAA 
CAACAAGCAC AAAATGGACA GAATGGTGAG CCCAGCAGCG TGGATGGGAA TTCTGGGGAT 
ACAGACTGCT TTCAGCCAGC AGTCAAAAGG GCAAAGTTGC AGGAGTCCAT TGAATATGAA 
GACTTGGGAA ACAACAATTC TGTGAAAACG ATTGCACTGA ATCTCAAGAA GTCAGACAGG 
TATTACCATG GTCCTACTCC AATTCAGTCA CTGCAGTATG CAACAAGTCA GGACATTATT 
AACTCTTTTC AAAGTATTAG ACAAGAAATG GAAGCTTACA CACCCAAGTT AACTCAGGTT 
CTCTCAAGCA GTGCTGCTAG TAGCACCATC ACAGCCCTGT CACCTGGAGG AGCTCTTATG 
CAGGGAGGGA CACAGCAAGC CGTAAACCAG ATGGTGCCAA ACGATATTCA GTCTGAATTG 
AAACACTTGT ATGTGGCTGT TGGGGAACTT CTGCGGCATT TTTGGTCTTG CTTTCCTGTT 
AATACACCAT TCCTAGAAGA AAAGGTAGTG AAAATGAAGA GTAATTTGGA ACGGTTCCAA 
GTTACAAAGC TCTGCCCATT CCAAGAAAAG ATTCGGAGGC AGTATTTAAG CACAAATCTG 
GTAAGTCACA TAGAAGAGAT GCTGCAGACA GCCTACAACA AGCTCCACGC GTGGCAATCC 
CGGCGCCTGA TGAAGAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032212.3
CDS	211..1854
Translation	MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHAWQSRRLMKKT

Target ORF information:

RefSeq Version	NM_008186.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008186.4

ORF Insert Sequence:

ATGGCGACTT CATCAGAAGA AGTGTTGCTG ATTGTGAAGA AGGTTCGTCA GAAGAAGCAG 
GATGGAGCAC TGTACCTCAT GGCAGAGAGA ATTGCTTGGG CACCTGAAGG CAAAGACAGA 
TTTACAATCA GCCATATGTA TGCAGATATT AAATGCCAGA AAATCAGTCC AGAGGGAAAA 
GCTAAAATAC AACTTCAGCT GGTCCTGCAT GCAGGGGACA CAACAAACTT CCATTTTTCC 
AACGAAAGCA CGGCAGTGAA AGAACGGGAT GCAGTGAAGG ACCTCCTTCA GCAGCTGCTG 
CCCAAGTTCA AGCGGAAAGC TAATAAAGAG CTGGAGGAGA AGAACAGAAT GCTCCAAGAA 
GATCCTGTTT TATTCCAACT CTATAAAGAC CTTGTTGTGA GCCAAGTGAT CAGTGCTGAG 
GAATTCTGGG CCAATCGTTT AAATGTGAAT GCAACAGATA GTTCTACATC CAGTCACAAG 
CAGGATGTTG GTATTTCTGC AGCATTTCTG GCTGATGTCC GGCCCCAAAC GGATGGCTGT 
AATGGTCTGA GATATAATTT AACTTCTGAT ATCATTGAAT CTATATTTAG GACCTATCCA 
GCAGTAAAAA TGAAATATGC AGAAACTGTA CCACACAACA TGACAGAAAA GGAGTTCTGG 
ACACGCTTTT TCCAGTCCCA TTATTTTCAC AGGGACCGAC TAAATACAGG ATCGAAGGAC 
CTCTTTGCAG AATGTGCCAA AATAGATGAG AAGGGATTAA AAACAATGGT TTCATTAGGA 
GTGAAAAACC CAATGCTAGA TTTGACATCG TTGGAAGATA AGCCATTAGA CGAGGGCTAT 
GGCATTTCCT CTGTGCCATC TACTTCCAAT TCCAAATCCA TAAAGGAGAA TAGTAATGCT 
GCCATCATCA AAAGGTTTAA CCACCACAGT GCCATGGTCC TGGCTGCTGG TCTCAGGAAA 
CAACAAGCAC AAAATGGACA GAATGGTGAG CCCAGCAGCG TGGATGGGAA TTCTGGGGAT 
ACAGACTGCT TTCAGCCAGC AGTCAAAAGG GCAAAGTTGC AGGAGTCCAT TGAATATGAA 
GACTTGGGAA ACAACAATTC TGTGAAAACG ATTGCACTGA ATCTCAAGAA GTCAGACAGG 
TATTACCATG GTCCTACTCC AATTCAGTCA CTGCAGTATG CAACAAGTCA GGACATTATT 
AACTCTTTTC AAAGTATTAG ACAAGAAATG GAAGCTTACA CACCCAAGTT AACTCAGGTT 
CTCTCAAGCA GTGCTGCTAG TAGCACCATC ACAGCCCTGT CACCTGGAGG AGCTCTTATG 
CAGGGAGGGA CACAGCAAGC CGTAAACCAG ATGGTGCCAA ACGATATTCA GTCTGAATTG 
AAACACTTGT ATGTGGCTGT TGGGGAACTT CTGCGGCATT TTTGGTCTTG CTTTCCTGTT 
AATACACCAT TCCTAGAAGA AAAGGTAGTG AAAATGAAGA GTAATTTGGA ACGGTTCCAA 
GTTACAAAGC TCTGCCCATT CCAAGAAAAG ATTCGGAGGC AGTATTTAAG CACAAATCTG 
GTAAGTCACA TAGAAGAGAT GCTGCAGACA GCCTACAACA AGCTCCACGC GTGGCAATCC 
CGGCGCCTGA TGAAGAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2h1 ( NM_001360076.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347005.1 Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu14443
Clone ID Related Accession (Same CDS sequence)	XM_006540636.3 , NM_001360076.1 , NM_001291075.1
Accession Version	NM_001360076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	general transcription factor IIH subunit 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090122.26 and AC090123.68. On Jan 26, 2018 this sequence version replaced XM_006540636.3. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (1) has a shorter and distinct N-terminus compared to isoform 3. Variants 1 and 4 both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.4652359.1, SRR1660825.170780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCTTA TAGCCATCAT GGCGACTTCA TCAGAAGAAG TGTTGCTGAT TGTGAAGAAG 
GTTCGTCAGA AGAAGCAGGA TGGAGCACTG TACCTCATGG CAGAGAGAAT TGCTTGGGCA 
CCTGAAGGCA AAGACAGATT TACAATCAGC CATATGTATG CAGATATTAA ATGCCAGAAA 
ATCAGTCCAG AGGGAAAAGC TAAAATACAA CTTCAGCTGG TCCTGCATGC AGGGGACACA 
ACAAACTTCC ATTTTTCCAA CGAAAGCACG GCAGTGAAAG AACGGGATGC AGTGAAGGAC 
CTCCTTCAGC AGCTGCTGCC CAAGTTCAAG CGGAAAGCTA ATAAAGAGCT GGAGGAGAAG 
AACAGAATGC TCCAAGAAGA TCCTGTTTTA TTCCAACTCT ATAAAGACCT TGTTGTGAGC 
CAAGTGATCA GTGCTGAGGA ATTCTGGGCC AATCGTTTAA ATGTGAATGC AACAGATAGT 
TCTACATCCA GTCACAAGCA GGATGTTGGT ATTTCTGCAG CATTTCTGGC TGATGTCCGG 
CCCCAAACGG ATGGCTGTAA TGGTCTGAGA TATAATTTAA CTTCTGATAT CATTGAATCT 
ATATTTAGGA CCTATCCAGC AGTAAAAATG AAATATGCAG AAACTGTACC ACACAACATG 
ACAGAAAAGG AGTTCTGGAC ACGCTTTTTC CAGTCCCATT ATTTTCACAG GGACCGACTA 
AATACAGGAT CGAAGGACCT CTTTGCAGAA TGTGCCAAAA TAGATGAGAA GGGATTAAAA 
ACAATGGTTT CATTAGGAGT GAAAAACCCA ATGCTAGATT TGACATCGTT GGAAGATAAG 
CCATTAGACG AGGGCTATGG CATTTCCTCT GTGCCATCTA CTTCCAATTC CAAATCCATA 
AAGGAGAATA GTAATGCTGC CATCATCAAA AGGTTTAACC ACCACAGTGC CATGGTCCTG 
GCTGCTGGTC TCAGGAAACA ACAAGCACAA AATGGACAGA ATGGTGAGCC CAGCAGCGTG 
GATGGGAATT CTGGGGATAC AGACTGCTTT CAGCCAGCAG TCAAAAGGGC AAAGTTGCAG 
GAGTCCATTG AATATGAAGA CTTGGGAAAC AACAATTCTG TGAAAACGAT TGCACTGAAT 
CTCAAGAAGT CAGACAGGTA TTACCATGGT CCTACTCCAA TTCAGTCACT GCAGTATGCA 
ACAAGTCAGG ACATTATTAA CTCTTTTCAA AGTATTAGAC AAGAAATGGA AGCTTACACA 
CCCAAGTTAA CTCAGGTTCT CTCAAGCAGT GCTGCTAGTA GCACCATCAC AGCCCTGTCA 
CCTGGAGGAG CTCTTATGCA GGGAGGGACA CAGCAAGCCG TAAACCAGAT GGTGCCAAAC 
GATATTCAGT CTGAATTGAA ACACTTGTAT GTGGCTGTTG GGGAACTTCT GCGGCATTTT 
TGGTCTTGCT TTCCTGTTAA TACACCATTC CTAGAAGAAA AGGTAGTGAA AATGAAGAGT 
AATTTGGAAC GGTTCCAAGT TACAAAGCTC TGCCCATTCC AAGAAAAGAT TCGGAGGCAG 
TATTTAAGCA CAAATCTGGT AAGTCACATA GAAGAGATGC TGCAGACAGC CTACAACAAG 
CTCCACGCGT GGCAATCCCG GCGCCTGATG AAGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347005.1
CDS	262..1923
Translation	MHLIAIMATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHAWQSRRLMKKT

Target ORF information:

RefSeq Version	NM_001360076.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360076.1

ORF Insert Sequence:

ATGCACCTTA TAGCCATCAT GGCGACTTCA TCAGAAGAAG TGTTGCTGAT TGTGAAGAAG 
GTTCGTCAGA AGAAGCAGGA TGGAGCACTG TACCTCATGG CAGAGAGAAT TGCTTGGGCA 
CCTGAAGGCA AAGACAGATT TACAATCAGC CATATGTATG CAGATATTAA ATGCCAGAAA 
ATCAGTCCAG AGGGAAAAGC TAAAATACAA CTTCAGCTGG TCCTGCATGC AGGGGACACA 
ACAAACTTCC ATTTTTCCAA CGAAAGCACG GCAGTGAAAG AACGGGATGC AGTGAAGGAC 
CTCCTTCAGC AGCTGCTGCC CAAGTTCAAG CGGAAAGCTA ATAAAGAGCT GGAGGAGAAG 
AACAGAATGC TCCAAGAAGA TCCTGTTTTA TTCCAACTCT ATAAAGACCT TGTTGTGAGC 
CAAGTGATCA GTGCTGAGGA ATTCTGGGCC AATCGTTTAA ATGTGAATGC AACAGATAGT 
TCTACATCCA GTCACAAGCA GGATGTTGGT ATTTCTGCAG CATTTCTGGC TGATGTCCGG 
CCCCAAACGG ATGGCTGTAA TGGTCTGAGA TATAATTTAA CTTCTGATAT CATTGAATCT 
ATATTTAGGA CCTATCCAGC AGTAAAAATG AAATATGCAG AAACTGTACC ACACAACATG 
ACAGAAAAGG AGTTCTGGAC ACGCTTTTTC CAGTCCCATT ATTTTCACAG GGACCGACTA 
AATACAGGAT CGAAGGACCT CTTTGCAGAA TGTGCCAAAA TAGATGAGAA GGGATTAAAA 
ACAATGGTTT CATTAGGAGT GAAAAACCCA ATGCTAGATT TGACATCGTT GGAAGATAAG 
CCATTAGACG AGGGCTATGG CATTTCCTCT GTGCCATCTA CTTCCAATTC CAAATCCATA 
AAGGAGAATA GTAATGCTGC CATCATCAAA AGGTTTAACC ACCACAGTGC CATGGTCCTG 
GCTGCTGGTC TCAGGAAACA ACAAGCACAA AATGGACAGA ATGGTGAGCC CAGCAGCGTG 
GATGGGAATT CTGGGGATAC AGACTGCTTT CAGCCAGCAG TCAAAAGGGC AAAGTTGCAG 
GAGTCCATTG AATATGAAGA CTTGGGAAAC AACAATTCTG TGAAAACGAT TGCACTGAAT 
CTCAAGAAGT CAGACAGGTA TTACCATGGT CCTACTCCAA TTCAGTCACT GCAGTATGCA 
ACAAGTCAGG ACATTATTAA CTCTTTTCAA AGTATTAGAC AAGAAATGGA AGCTTACACA 
CCCAAGTTAA CTCAGGTTCT CTCAAGCAGT GCTGCTAGTA GCACCATCAC AGCCCTGTCA 
CCTGGAGGAG CTCTTATGCA GGGAGGGACA CAGCAAGCCG TAAACCAGAT GGTGCCAAAC 
GATATTCAGT CTGAATTGAA ACACTTGTAT GTGGCTGTTG GGGAACTTCT GCGGCATTTT 
TGGTCTTGCT TTCCTGTTAA TACACCATTC CTAGAAGAAA AGGTAGTGAA AATGAAGAGT 
AATTTGGAAC GGTTCCAAGT TACAAAGCTC TGCCCATTCC AAGAAAAGAT TCGGAGGCAG 
TATTTAAGCA CAAATCTGGT AAGTCACATA GAAGAGATGC TGCAGACAGC CTACAACAAG 
CTCCACGCGT GGCAATCCCG GCGCCTGATG AAGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2h1 ( NM_001291075.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278004.1 Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14443
Clone ID Related Accession (Same CDS sequence)	XM_006540636.3 , NM_001360076.1 , NM_001291075.1
Accession Version	NM_001291075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	general transcription factor IIH subunit 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052837.1, AK005065.1, AC090123.68 and BE136268.1. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (1) has a shorter and distinct N-terminus compared to isoform 3. Variants 1 and 4 both encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCTTA TAGCCATCAT GGCGACTTCA TCAGAAGAAG TGTTGCTGAT TGTGAAGAAG 
GTTCGTCAGA AGAAGCAGGA TGGAGCACTG TACCTCATGG CAGAGAGAAT TGCTTGGGCA 
CCTGAAGGCA AAGACAGATT TACAATCAGC CATATGTATG CAGATATTAA ATGCCAGAAA 
ATCAGTCCAG AGGGAAAAGC TAAAATACAA CTTCAGCTGG TCCTGCATGC AGGGGACACA 
ACAAACTTCC ATTTTTCCAA CGAAAGCACG GCAGTGAAAG AACGGGATGC AGTGAAGGAC 
CTCCTTCAGC AGCTGCTGCC CAAGTTCAAG CGGAAAGCTA ATAAAGAGCT GGAGGAGAAG 
AACAGAATGC TCCAAGAAGA TCCTGTTTTA TTCCAACTCT ATAAAGACCT TGTTGTGAGC 
CAAGTGATCA GTGCTGAGGA ATTCTGGGCC AATCGTTTAA ATGTGAATGC AACAGATAGT 
TCTACATCCA GTCACAAGCA GGATGTTGGT ATTTCTGCAG CATTTCTGGC TGATGTCCGG 
CCCCAAACGG ATGGCTGTAA TGGTCTGAGA TATAATTTAA CTTCTGATAT CATTGAATCT 
ATATTTAGGA CCTATCCAGC AGTAAAAATG AAATATGCAG AAACTGTACC ACACAACATG 
ACAGAAAAGG AGTTCTGGAC ACGCTTTTTC CAGTCCCATT ATTTTCACAG GGACCGACTA 
AATACAGGAT CGAAGGACCT CTTTGCAGAA TGTGCCAAAA TAGATGAGAA GGGATTAAAA 
ACAATGGTTT CATTAGGAGT GAAAAACCCA ATGCTAGATT TGACATCGTT GGAAGATAAG 
CCATTAGACG AGGGCTATGG CATTTCCTCT GTGCCATCTA CTTCCAATTC CAAATCCATA 
AAGGAGAATA GTAATGCTGC CATCATCAAA AGGTTTAACC ACCACAGTGC CATGGTCCTG 
GCTGCTGGTC TCAGGAAACA ACAAGCACAA AATGGACAGA ATGGTGAGCC CAGCAGCGTG 
GATGGGAATT CTGGGGATAC AGACTGCTTT CAGCCAGCAG TCAAAAGGGC AAAGTTGCAG 
GAGTCCATTG AATATGAAGA CTTGGGAAAC AACAATTCTG TGAAAACGAT TGCACTGAAT 
CTCAAGAAGT CAGACAGGTA TTACCATGGT CCTACTCCAA TTCAGTCACT GCAGTATGCA 
ACAAGTCAGG ACATTATTAA CTCTTTTCAA AGTATTAGAC AAGAAATGGA AGCTTACACA 
CCCAAGTTAA CTCAGGTTCT CTCAAGCAGT GCTGCTAGTA GCACCATCAC AGCCCTGTCA 
CCTGGAGGAG CTCTTATGCA GGGAGGGACA CAGCAAGCCG TAAACCAGAT GGTGCCAAAC 
GATATTCAGT CTGAATTGAA ACACTTGTAT GTGGCTGTTG GGGAACTTCT GCGGCATTTT 
TGGTCTTGCT TTCCTGTTAA TACACCATTC CTAGAAGAAA AGGTAGTGAA AATGAAGAGT 
AATTTGGAAC GGTTCCAAGT TACAAAGCTC TGCCCATTCC AAGAAAAGAT TCGGAGGCAG 
TATTTAAGCA CAAATCTGGT AAGTCACATA GAAGAGATGC TGCAGACAGC CTACAACAAG 
CTCCACGCGT GGCAATCCCG GCGCCTGATG AAGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278004.1
CDS	160..1821
Translation	MHLIAIMATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHAWQSRRLMKKT

Target ORF information:

RefSeq Version	NM_001291075.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291075.1

ORF Insert Sequence:

ATGCACCTTA TAGCCATCAT GGCGACTTCA TCAGAAGAAG TGTTGCTGAT TGTGAAGAAG 
GTTCGTCAGA AGAAGCAGGA TGGAGCACTG TACCTCATGG CAGAGAGAAT TGCTTGGGCA 
CCTGAAGGCA AAGACAGATT TACAATCAGC CATATGTATG CAGATATTAA ATGCCAGAAA 
ATCAGTCCAG AGGGAAAAGC TAAAATACAA CTTCAGCTGG TCCTGCATGC AGGGGACACA 
ACAAACTTCC ATTTTTCCAA CGAAAGCACG GCAGTGAAAG AACGGGATGC AGTGAAGGAC 
CTCCTTCAGC AGCTGCTGCC CAAGTTCAAG CGGAAAGCTA ATAAAGAGCT GGAGGAGAAG 
AACAGAATGC TCCAAGAAGA TCCTGTTTTA TTCCAACTCT ATAAAGACCT TGTTGTGAGC 
CAAGTGATCA GTGCTGAGGA ATTCTGGGCC AATCGTTTAA ATGTGAATGC AACAGATAGT 
TCTACATCCA GTCACAAGCA GGATGTTGGT ATTTCTGCAG CATTTCTGGC TGATGTCCGG 
CCCCAAACGG ATGGCTGTAA TGGTCTGAGA TATAATTTAA CTTCTGATAT CATTGAATCT 
ATATTTAGGA CCTATCCAGC AGTAAAAATG AAATATGCAG AAACTGTACC ACACAACATG 
ACAGAAAAGG AGTTCTGGAC ACGCTTTTTC CAGTCCCATT ATTTTCACAG GGACCGACTA 
AATACAGGAT CGAAGGACCT CTTTGCAGAA TGTGCCAAAA TAGATGAGAA GGGATTAAAA 
ACAATGGTTT CATTAGGAGT GAAAAACCCA ATGCTAGATT TGACATCGTT GGAAGATAAG 
CCATTAGACG AGGGCTATGG CATTTCCTCT GTGCCATCTA CTTCCAATTC CAAATCCATA 
AAGGAGAATA GTAATGCTGC CATCATCAAA AGGTTTAACC ACCACAGTGC CATGGTCCTG 
GCTGCTGGTC TCAGGAAACA ACAAGCACAA AATGGACAGA ATGGTGAGCC CAGCAGCGTG 
GATGGGAATT CTGGGGATAC AGACTGCTTT CAGCCAGCAG TCAAAAGGGC AAAGTTGCAG 
GAGTCCATTG AATATGAAGA CTTGGGAAAC AACAATTCTG TGAAAACGAT TGCACTGAAT 
CTCAAGAAGT CAGACAGGTA TTACCATGGT CCTACTCCAA TTCAGTCACT GCAGTATGCA 
ACAAGTCAGG ACATTATTAA CTCTTTTCAA AGTATTAGAC AAGAAATGGA AGCTTACACA 
CCCAAGTTAA CTCAGGTTCT CTCAAGCAGT GCTGCTAGTA GCACCATCAC AGCCCTGTCA 
CCTGGAGGAG CTCTTATGCA GGGAGGGACA CAGCAAGCCG TAAACCAGAT GGTGCCAAAC 
GATATTCAGT CTGAATTGAA ACACTTGTAT GTGGCTGTTG GGGAACTTCT GCGGCATTTT 
TGGTCTTGCT TTCCTGTTAA TACACCATTC CTAGAAGAAA AGGTAGTGAA AATGAAGAGT 
AATTTGGAAC GGTTCCAAGT TACAAAGCTC TGCCCATTCC AAGAAAAGAT TCGGAGGCAG 
TATTTAAGCA CAAATCTGGT AAGTCACATA GAAGAGATGC TGCAGACAGC CTACAACAAG 
CTCCACGCGT GGCAATCCCG GCGCCTGATG AAGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2h1 ( XM_006540636.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540699.1 Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant X1, mRNA.

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CloneID	OMu14443
Clone ID Related Accession (Same CDS sequence)	XM_006540636.3 , NM_001360076.1 , NM_001291075.1
Accession Version	XM_006540636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	general transcription factor IIH subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTTA TAGCCATCAT GGCGACTTCA TCAGAAGAAG TGTTGCTGAT TGTGAAGAAG 
GTTCGTCAGA AGAAGCAGGA TGGAGCACTG TACCTCATGG CAGAGAGAAT TGCTTGGGCA 
CCTGAAGGCA AAGACAGATT TACAATCAGC CATATGTATG CAGATATTAA ATGCCAGAAA 
ATCAGTCCAG AGGGAAAAGC TAAAATACAA CTTCAGCTGG TCCTGCATGC AGGGGACACA 
ACAAACTTCC ATTTTTCCAA CGAAAGCACG GCAGTGAAAG AACGGGATGC AGTGAAGGAC 
CTCCTTCAGC AGCTGCTGCC CAAGTTCAAG CGGAAAGCTA ATAAAGAGCT GGAGGAGAAG 
AACAGAATGC TCCAAGAAGA TCCTGTTTTA TTCCAACTCT ATAAAGACCT TGTTGTGAGC 
CAAGTGATCA GTGCTGAGGA ATTCTGGGCC AATCGTTTAA ATGTGAATGC AACAGATAGT 
TCTACATCCA GTCACAAGCA GGATGTTGGT ATTTCTGCAG CATTTCTGGC TGATGTCCGG 
CCCCAAACGG ATGGCTGTAA TGGTCTGAGA TATAATTTAA CTTCTGATAT CATTGAATCT 
ATATTTAGGA CCTATCCAGC AGTAAAAATG AAATATGCAG AAACTGTACC ACACAACATG 
ACAGAAAAGG AGTTCTGGAC ACGCTTTTTC CAGTCCCATT ATTTTCACAG GGACCGACTA 
AATACAGGAT CGAAGGACCT CTTTGCAGAA TGTGCCAAAA TAGATGAGAA GGGATTAAAA 
ACAATGGTTT CATTAGGAGT GAAAAACCCA ATGCTAGATT TGACATCGTT GGAAGATAAG 
CCATTAGACG AGGGCTATGG CATTTCCTCT GTGCCATCTA CTTCCAATTC CAAATCCATA 
AAGGAGAATA GTAATGCTGC CATCATCAAA AGGTTTAACC ACCACAGTGC CATGGTCCTG 
GCTGCTGGTC TCAGGAAACA ACAAGCACAA AATGGACAGA ATGGTGAGCC CAGCAGCGTG 
GATGGGAATT CTGGGGATAC AGACTGCTTT CAGCCAGCAG TCAAAAGGGC AAAGTTGCAG 
GAGTCCATTG AATATGAAGA CTTGGGAAAC AACAATTCTG TGAAAACGAT TGCACTGAAT 
CTCAAGAAGT CAGACAGGTA TTACCATGGT CCTACTCCAA TTCAGTCACT GCAGTATGCA 
ACAAGTCAGG ACATTATTAA CTCTTTTCAA AGTATTAGAC AAGAAATGGA AGCTTACACA 
CCCAAGTTAA CTCAGGTTCT CTCAAGCAGT GCTGCTAGTA GCACCATCAC AGCCCTGTCA 
CCTGGAGGAG CTCTTATGCA GGGAGGGACA CAGCAAGCCG TAAACCAGAT GGTGCCAAAC 
GATATTCAGT CTGAATTGAA ACACTTGTAT GTGGCTGTTG GGGAACTTCT GCGGCATTTT 
TGGTCTTGCT TTCCTGTTAA TACACCATTC CTAGAAGAAA AGGTAGTGAA AATGAAGAGT 
AATTTGGAAC GGTTCCAAGT TACAAAGCTC TGCCCATTCC AAGAAAAGAT TCGGAGGCAG 
TATTTAAGCA CAAATCTGGT AAGTCACATA GAAGAGATGC TGCAGACAGC CTACAACAAG 
CTCCACGCGT GGCAATCCCG GCGCCTGATG AAGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540699.1
CDS	159..1820
Translation	MHLIAIMATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHAWQSRRLMKKT

Target ORF information:

RefSeq Version	XM_006540636.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540636.3

ORF Insert Sequence:

ATGCACCTTA TAGCCATCAT GGCGACTTCA TCAGAAGAAG TGTTGCTGAT TGTGAAGAAG 
GTTCGTCAGA AGAAGCAGGA TGGAGCACTG TACCTCATGG CAGAGAGAAT TGCTTGGGCA 
CCTGAAGGCA AAGACAGATT TACAATCAGC CATATGTATG CAGATATTAA ATGCCAGAAA 
ATCAGTCCAG AGGGAAAAGC TAAAATACAA CTTCAGCTGG TCCTGCATGC AGGGGACACA 
ACAAACTTCC ATTTTTCCAA CGAAAGCACG GCAGTGAAAG AACGGGATGC AGTGAAGGAC 
CTCCTTCAGC AGCTGCTGCC CAAGTTCAAG CGGAAAGCTA ATAAAGAGCT GGAGGAGAAG 
AACAGAATGC TCCAAGAAGA TCCTGTTTTA TTCCAACTCT ATAAAGACCT TGTTGTGAGC 
CAAGTGATCA GTGCTGAGGA ATTCTGGGCC AATCGTTTAA ATGTGAATGC AACAGATAGT 
TCTACATCCA GTCACAAGCA GGATGTTGGT ATTTCTGCAG CATTTCTGGC TGATGTCCGG 
CCCCAAACGG ATGGCTGTAA TGGTCTGAGA TATAATTTAA CTTCTGATAT CATTGAATCT 
ATATTTAGGA CCTATCCAGC AGTAAAAATG AAATATGCAG AAACTGTACC ACACAACATG 
ACAGAAAAGG AGTTCTGGAC ACGCTTTTTC CAGTCCCATT ATTTTCACAG GGACCGACTA 
AATACAGGAT CGAAGGACCT CTTTGCAGAA TGTGCCAAAA TAGATGAGAA GGGATTAAAA 
ACAATGGTTT CATTAGGAGT GAAAAACCCA ATGCTAGATT TGACATCGTT GGAAGATAAG 
CCATTAGACG AGGGCTATGG CATTTCCTCT GTGCCATCTA CTTCCAATTC CAAATCCATA 
AAGGAGAATA GTAATGCTGC CATCATCAAA AGGTTTAACC ACCACAGTGC CATGGTCCTG 
GCTGCTGGTC TCAGGAAACA ACAAGCACAA AATGGACAGA ATGGTGAGCC CAGCAGCGTG 
GATGGGAATT CTGGGGATAC AGACTGCTTT CAGCCAGCAG TCAAAAGGGC AAAGTTGCAG 
GAGTCCATTG AATATGAAGA CTTGGGAAAC AACAATTCTG TGAAAACGAT TGCACTGAAT 
CTCAAGAAGT CAGACAGGTA TTACCATGGT CCTACTCCAA TTCAGTCACT GCAGTATGCA 
ACAAGTCAGG ACATTATTAA CTCTTTTCAA AGTATTAGAC AAGAAATGGA AGCTTACACA 
CCCAAGTTAA CTCAGGTTCT CTCAAGCAGT GCTGCTAGTA GCACCATCAC AGCCCTGTCA 
CCTGGAGGAG CTCTTATGCA GGGAGGGACA CAGCAAGCCG TAAACCAGAT GGTGCCAAAC 
GATATTCAGT CTGAATTGAA ACACTTGTAT GTGGCTGTTG GGGAACTTCT GCGGCATTTT 
TGGTCTTGCT TTCCTGTTAA TACACCATTC CTAGAAGAAA AGGTAGTGAA AATGAAGAGT 
AATTTGGAAC GGTTCCAAGT TACAAAGCTC TGCCCATTCC AAGAAAAGAT TCGGAGGCAG 
TATTTAAGCA CAAATCTGGT AAGTCACATA GAAGAGATGC TGCAGACAGC CTACAACAAG 
CTCCACGCGT GGCAATCCCG GCGCCTGATG AAGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2h1 ( NM_001360075.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347004.1 Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 3, mRNA.

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CloneID	OMu84036
Clone ID Related Accession (Same CDS sequence)	NM_001360075.1
Accession Version	NM_001360075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	general transcription factor IIH subunit 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090122.26 and AC090123.68. Transcript Variant: This variant (3) encodes the longest isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.174409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACACC TTATAGCCAT CATGGCGACT TCATCAGAAG AAGTGTTGCT GATTGTGAAG 
AAGGTTCGTC AGAAGAAGCA GGATGGAGCA CTGTACCTCA TGGCAGAGAG AATTGCTTGG 
GCACCTGAAG GCAAAGACAG ATTTACAATC AGCCATATGT ATGCAGATAT TAAATGCCAG 
AAAATCAGTC CAGAGGGAAA AGCTAAAATA CAACTTCAGC TGGTCCTGCA TGCAGGGGAC 
ACAACAAACT TCCATTTTTC CAACGAAAGC ACGGCAGTGA AAGAACGGGA TGCAGTGAAG 
GACCTCCTTC AGCAGCTGCT GCCCAAGTTC AAGCGGAAAG CTAATAAAGA GCTGGAGGAG 
AAGAACAGAA TGCTCCAAGA AGATCCTGTT TTATTCCAAC TCTATAAAGA CCTTGTTGTG 
AGCCAAGTGA TCAGTGCTGA GGAATTCTGG GCCAATCGTT TAAATGTGAA TGCAACAGAT 
AGTTCTACAT CCAGTCACAA GCAGGATGTT GGTATTTCTG CAGCATTTCT GGCTGATGTC 
CGGCCCCAAA CGGATGGCTG TAATGGTCTG AGATATAATT TAACTTCTGA TATCATTGAA 
TCTATATTTA GGACCTATCC AGCAGTAAAA ATGAAATATG CAGAAACTGT ACCACACAAC 
ATGACAGAAA AGGAGTTCTG GACACGCTTT TTCCAGTCCC ATTATTTTCA CAGGGACCGA 
CTAAATACAG GATCGAAGGA CCTCTTTGCA GAATGTGCCA AAATAGATGA GAAGGGATTA 
AAAACAATGG TTTCATTAGG AGTGAAAAAC CCAATGCTAG ATTTGACATC GTTGGAAGAT 
AAGCCATTAG ACGAGGGCTA TGGCATTTCC TCTGTGCCAT CTACTTCCAA TTCCAAATCC 
ATAAAGGAGA ATAGTAATGC TGCCATCATC AAAAGGTTTA ACCACCACAG TGCCATGGTC 
CTGGCTGCTG GTCTCAGGAA ACAACAAGCA CAAAATGGAC AGAATGGTGA GCCCAGCAGC 
GTGGATGGGA ATTCTGGGGA TACAGACTGC TTTCAGCCAG CAGTCAAAAG GGCAAAGTTG 
CAGGAGTCCA TTGAATATGA AGACTTGGGA AACAACAATT CTGTGAAAAC GATTGCACTG 
AATCTCAAGA AGTCAGACAG GTATTACCAT GGTCCTACTC CAATTCAGTC ACTGCAGTAT 
GCAACAAGTC AGGACATTAT TAACTCTTTT CAAAGTATTA GACAAGAAAT GGAAGCTTAC 
ACACCCAAGT TAACTCAGGT TCTCTCAAGC AGTGCTGCTA GTAGCACCAT CACAGCCCTG 
TCACCTGGAG GAGCTCTTAT GCAGGGAGGG ACACAGCAAG CCGTAAACCA GATGGTGCCA 
AACGATATTC AGTCTGAATT GAAACACTTG TATGTGGCTG TTGGGGAACT TCTGCGGCAT 
TTTTGGTCTT GCTTTCCTGT TAATACACCA TTCCTAGAAG AAAAGGTAGT GAAAATGAAG 
AGTAATTTGG AACGGTTCCA AGTTACAAAG CTCTGCCCAT TCCAAGAAAA GATTCGGAGG 
CAGTATTTAA GCACAAATCT GGTAAGTCAC ATAGAAGAGA TGCTGCAGAC AGCCTACAAC 
AAGCTCCACG CGTGGCAATC CCGGCGCCTG ATGAAGAAGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347004.1
CDS	88..1752
Translation	MEHLIAIMATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHAWQSRRLMKKT

Target ORF information:

RefSeq Version	NM_001360075.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor II H, polypeptide 1 (Gtf2h1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360075.1

ORF Insert Sequence:

ATGGAACACC TTATAGCCAT CATGGCGACT TCATCAGAAG AAGTGTTGCT GATTGTGAAG 
AAGGTTCGTC AGAAGAAGCA GGATGGAGCA CTGTACCTCA TGGCAGAGAG AATTGCTTGG 
GCACCTGAAG GCAAAGACAG ATTTACAATC AGCCATATGT ATGCAGATAT TAAATGCCAG 
AAAATCAGTC CAGAGGGAAA AGCTAAAATA CAACTTCAGC TGGTCCTGCA TGCAGGGGAC 
ACAACAAACT TCCATTTTTC CAACGAAAGC ACGGCAGTGA AAGAACGGGA TGCAGTGAAG 
GACCTCCTTC AGCAGCTGCT GCCCAAGTTC AAGCGGAAAG CTAATAAAGA GCTGGAGGAG 
AAGAACAGAA TGCTCCAAGA AGATCCTGTT TTATTCCAAC TCTATAAAGA CCTTGTTGTG 
AGCCAAGTGA TCAGTGCTGA GGAATTCTGG GCCAATCGTT TAAATGTGAA TGCAACAGAT 
AGTTCTACAT CCAGTCACAA GCAGGATGTT GGTATTTCTG CAGCATTTCT GGCTGATGTC 
CGGCCCCAAA CGGATGGCTG TAATGGTCTG AGATATAATT TAACTTCTGA TATCATTGAA 
TCTATATTTA GGACCTATCC AGCAGTAAAA ATGAAATATG CAGAAACTGT ACCACACAAC 
ATGACAGAAA AGGAGTTCTG GACACGCTTT TTCCAGTCCC ATTATTTTCA CAGGGACCGA 
CTAAATACAG GATCGAAGGA CCTCTTTGCA GAATGTGCCA AAATAGATGA GAAGGGATTA 
AAAACAATGG TTTCATTAGG AGTGAAAAAC CCAATGCTAG ATTTGACATC GTTGGAAGAT 
AAGCCATTAG ACGAGGGCTA TGGCATTTCC TCTGTGCCAT CTACTTCCAA TTCCAAATCC 
ATAAAGGAGA ATAGTAATGC TGCCATCATC AAAAGGTTTA ACCACCACAG TGCCATGGTC 
CTGGCTGCTG GTCTCAGGAA ACAACAAGCA CAAAATGGAC AGAATGGTGA GCCCAGCAGC 
GTGGATGGGA ATTCTGGGGA TACAGACTGC TTTCAGCCAG CAGTCAAAAG GGCAAAGTTG 
CAGGAGTCCA TTGAATATGA AGACTTGGGA AACAACAATT CTGTGAAAAC GATTGCACTG 
AATCTCAAGA AGTCAGACAG GTATTACCAT GGTCCTACTC CAATTCAGTC ACTGCAGTAT 
GCAACAAGTC AGGACATTAT TAACTCTTTT CAAAGTATTA GACAAGAAAT GGAAGCTTAC 
ACACCCAAGT TAACTCAGGT TCTCTCAAGC AGTGCTGCTA GTAGCACCAT CACAGCCCTG 
TCACCTGGAG GAGCTCTTAT GCAGGGAGGG ACACAGCAAG CCGTAAACCA GATGGTGCCA 
AACGATATTC AGTCTGAATT GAAACACTTG TATGTGGCTG TTGGGGAACT TCTGCGGCAT 
TTTTGGTCTT GCTTTCCTGT TAATACACCA TTCCTAGAAG AAAAGGTAGT GAAAATGAAG 
AGTAATTTGG AACGGTTCCA AGTTACAAAG CTCTGCCCAT TCCAAGAAAA GATTCGGAGG 
CAGTATTTAA GCACAAATCT GGTAAGTCAC ATAGAAGAGA TGCTGCAGAC AGCCTACAAC 
AAGCTCCACG CGTGGCAATC CCGGCGCCTG ATGAAGAAGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The insulin-like growth factor 2 (IGF2) mRNA-binding protein p62/IGF2BP2-2 as a promoter of NAFLD and HCC?
Gut63(5)861-3(2014 May)
Simon Y,Kessler SM,Bohle RM,Haybaeck J,Kiemer AK

p62 expression and autophagy in ?B-crystallin R120G mutant knock-in mouse model of hereditary cataract.
Experimental eye research115263-73(2013 Oct)
Wignes JA,Goldman JW,Weihl CC,Bartley MG,Andley UP

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)
Kamileri I,Karakasilioti I,Sideri A,Kosteas T,Tatarakis A,Talianidis I,Garinis GA

Autophagy and p62 in cardiac proteinopathy.
Circulation research109(3)296-308(2011 Jul)
Zheng Q,Su H,Ranek MJ,Wang X

Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation.
Molecular and cellular biology31(1)105-17(2011 Jan)
Linares JF,Amanchy R,Greis K,Diaz-Meco MT,Moscat J

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Gtf2h1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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