tmed7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmed7
Entrez Gene ID	405848
Full Name	transmembrane p24 trafficking protein 7
Synonyms	zgc:76996
General protein information	Preferred Names transmembrane p24 trafficking protein 7 Names transmembrane emp24 domain-containing protein 7 p24 gamma 3 transmembrane emp24 protein transport domain containing 7
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313710.1	NP_001300639.1	transmembrane emp24 domain-containing protein 7 precursor
NM_001313709.1	NP_001300638.1	transmembrane emp24 domain-containing protein 7 precursor
NM_212912.2	NP_998077.2	transmembrane emp24 domain-containing protein 7 precursor
NM_001313708.1	NP_001300637.1	transmembrane emp24 domain-containing protein 7 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMED7	NP_861974.1
Mus musculus (house mouse)	Tmed7	NP_079974.1
Saccharomyces cerevisiae (baker's yeast)	ERP2	NP_009395.1
Pan troglodytes (chimpanzee)	TMED7	NP_001191238.1
Drosophila melanogaster (fruit fly)	p24-1	NP_001259465.1
Kluyveromyces lactis	KLLA0D14641g	XP_453709.1
Eremothecium gossypii	AGOS_ADR026W	NP_984122.1
Neurospora crassa	NCU03800	XP_961189.2
Canis lupus familiaris (dog)	TMED7	NP_001191267.1
Bos taurus (cattle)	TMED7	NP_001098966.1
Gallus gallus (chicken)	TMED7	XP_003643122.2
Caenorhabditis elegans (roundworm)	F57B10.5	NP_491892.1
Saccharomyces cerevisiae (baker's yeast)	ERP4	NP_014659.1
Schizosaccharomyces pombe (fission yeast)	SPAC17A5.08	NP_593476.1
Magnaporthe oryzae (rice blast fungus)	MGG_07471	XP_003711375.1
Xenopus tropicalis (tropical clawed frog)	tmed7	NP_989261.1
Rattus norvegicus (Norway rat)	Tmed7	NP_001099228.1
Danio rerio (zebrafish)	tmed7	NP_998077.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000949	XP_309138.3

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Label-free quantitative analysis of the membrane proteome of Bace1 protease knock-out zebrafish brains.
Hogl S, van Bebber F, Dislich B, Kuhn PH, Haass C, Schmid B, Lichtenthaler SF
Proteomics13(9)1519-27(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tmed7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmed7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13757	NM_212912.2 Latest version!	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa13757	NM_001313709.1 Latest version!	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa13757	NM_001313708.1 Latest version!	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa13757	NM_001313710.1 Latest version!	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmed7 ( NM_001313710.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001300639.1 Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 4, mRNA.

Price & Availability↑

CloneID	ODa13757
Clone ID Related Accession (Same CDS sequence)	NM_001313710.1 , NM_001313709.1 , NM_212912.2 , NM_001313708.1
Accession Version	NM_001313710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	transmembrane emp24 domain-containing protein 7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01087431.1, EH456276.1 and EH482012.1. On Aug 27, 2015 this sequence version replaced XM_005172427.2. Transcript Variant: This variant (4) uses an alternate 5' terminal exon and an alternate splice site that result in a different 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: GFIL01040923.1, EH456276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300639.1
CDS	101..751
Translation	MAGSSSSWLLPLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRKTTTTRMGS

Target ORF information:

RefSeq Version	NM_001313710.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313710.1

ORF Insert Sequence:

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmed7 ( NM_001313709.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001300638.1 Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa13757
Clone ID Related Accession (Same CDS sequence)	NM_001313710.1 , NM_001313709.1 , NM_212912.2 , NM_001313708.1
Accession Version	NM_001313709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	transmembrane emp24 domain-containing protein 7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN505306.1, CABZ01087431.1, EH456276.1 and EH482012.1. On Aug 27, 2015 this sequence version replaced XM_005172429.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC159190.1, EV604216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300638.1
CDS	189..839
Translation	MAGSSSSWLLPLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRKTTTTRMGS

Target ORF information:

RefSeq Version	NM_001313709.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313709.1

ORF Insert Sequence:

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmed7 ( NM_212912.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998077.2 Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ODa13757
Clone ID Related Accession (Same CDS sequence)	NM_001313710.1 , NM_001313709.1 , NM_212912.2 , NM_001313708.1
Accession Version	NM_212912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	transmembrane emp24 domain-containing protein 7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01087431.1, EH456276.1, DT881625.1 and EH482012.1. On Aug 27, 2015 this sequence version replaced NM_212912.1. Transcript Variant: This variant (3) uses an alternate 5' terminal exon, which results in a different 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: EE301360.1, BC067383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998077.2
CDS	104..754
Translation	MAGSSSSWLLPLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRKTTTTRMGS

Target ORF information:

RefSeq Version	NM_212912.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212912.2

ORF Insert Sequence:

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmed7 ( NM_001313708.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001300637.1 Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa13757
Clone ID Related Accession (Same CDS sequence)	NM_001313710.1 , NM_001313709.1 , NM_212912.2 , NM_001313708.1
Accession Version	NM_001313708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	transmembrane emp24 domain-containing protein 7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN505306.1, CABZ01087431.1, EH456276.1 and EH482012.1. On Aug 27, 2015 this sequence version replaced XM_005172428.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3, and 4 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: DT871649.1, CN505306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300637.1
CDS	192..842
Translation	MAGSSSSWLLPLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRKTTTTRMGS

Target ORF information:

RefSeq Version	NM_001313708.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane p24 trafficking protein 7 (tmed7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313708.1

ORF Insert Sequence:

ATGGCTGGCT CCTCATCTTC GTGGCTCCTC CCACTATTTG TTCAGGTGGT GATGATGAAG 
GTGGGCTTAA GCTCCGCCTC TGAGCTGACC TTCGAGCTGC CGGACAACGC CAAGCAGTGT 
TTCTATGAGG ACATCACCAT CGGCACCAAG TGCACACTCG AGTTCCAGGT GGTCACCGGG 
GGCCACTATG ACGTGGACTG CCGGCTGGAG GACCCCGAGG GGACGGTGCT CTACAAGGAG 
ATGAAGAAGC AGTACGACAG CTTCACCTTC AGTGCCGCTC GCAACGGAAC CTACAAGTTC 
TGCTTCAGCA ACGAGTTCTC CACCTTCACA CACAAGACCG TCTACTTCGA CTTCCAGGTG 
GGAGACGACC CGCCGCTCTT CCCCAACGAG AACCGGGTCA CGGCACTGAC ACAGATGGAG 
TCAGCGTGTG TGTCGATCCA TGAGGCGCTG AAGTCGGTGA TGGACTATCA GACACACTTC 
CGGCTGCGCG AGGCTCAGGG CCGCAGCCGC GCTGAAGACC TGAACAGCAG AGTGGCCTTC 
TGGTCGGTCG GGGAGGCGCT GATCCTGCTG GTGGTGAGCG TCAGTCAGGT GCTGCTGCTG 
CGCTCCTTCT TCTCCGACCG CAAGACCACC ACCACCCGCA TGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Label-free quantitative analysis of the membrane proteome of Bace1 protease knock-out zebrafish brains.
Proteomics13(9)1519-27(2013 May)
Hogl S,van Bebber F,Dislich B,Kuhn PH,Haass C,Schmid B,Lichtenthaler SF

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tmed7 cDNA ORF clone, Danio rerio(zebrafish)

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