tlr3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tlr3
Entrez Gene ID	403126
Full Name	toll-like receptor 3
Synonyms	zgc:123006
General protein information	Preferred Names toll-like receptor 3 Names toll-like receptor 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013269.3	NP_001013287.2	toll-like receptor 3 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TLR3	NP_001008664.1
Danio rerio (zebrafish)	tlr3	NP_001013287.2
Xenopus tropicalis (tropical clawed frog)	tlr3	XP_002934448.2
Homo sapiens (human)	TLR3	NP_003256.1
	TLR3	NP_001031762.1
Gallus gallus (chicken)	TLR3	NP_001011691.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012385	XP_551799.2
Pan troglodytes (chimpanzee)	TLR3	NP_001123942.1
Canis lupus familiaris (dog)	TLR3	XP_005630025.1
Rattus norvegicus (Norway rat)	Tlr3	NP_942086.1
Mus musculus (house mouse)	Tlr3	NP_569054.2
Drosophila melanogaster (fruit fly)	Tollo	NP_524757.1

Gene Ontology
Bibliography

Related articles in PubMed

Transgenic expression of tilapia piscidin 3 (TP3) in zebrafish confers resistance to Streptococcus agalactiae.
Su BC, Lai YW, Chen JY, Pan CY
Fish & shellfish immunology74235-241(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations
Title: Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations.

Binding energy (BE) calculation using MM/PBSA method from the TLR3- and TLR22-ligand complexes revealed an adequate binding affinity between TLR22-monomer and dsRNA as like as TLR3-dimer-dsRNA complex.
Title: Understanding the distinguishable structural and functional features in zebrafish TLR3 and TLR22, and their binding modes with fish dsRNA viruses: an exploratory structural model analysis.

Full-length tlr3 was functionally characterized.
Title: Functional characterization of full-length TLR3, IRAK-4, and TRAF6 in zebrafish (Danio rerio).

The following tlr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tlr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00693	NM_001013269.3 Latest version!	Danio rerio toll-like receptor 3 (tlr3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tlr3 ( NM_001013269.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001013287.2 Danio rerio toll-like receptor 3 (tlr3), mRNA.

Price & Availability↑

CloneID	ODa00693
Clone ID Related Accession (Same CDS sequence)	NM_001013269.3
Accession Version	NM_001013269.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-20
Organism	Danio rerio(zebrafish)
Product	toll-like receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107955.1. On Aug 30, 2012 this sequence version replaced NM_001013269.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATCTAA TGAAACTCAT ATTATTGCCC TTGTTCTACG CTTGTTTTAG TGCCCACTGT 
GCAGGCACTA CAAATGCACG CAAGTCAGCT TGCATGATTA AGAATGCCAA AGCAGACTGT 
AGTCATATGA ATCTGGATGC AATTCCAACG GACTTACCTA CAAATATTAC CACACTGGAT 
GTATCTCACA ACAGACTTAA AACTCTGTCT TCTCTACACA TGTACACAAA TCTGGTGAAT 
ATAGATGCGA GCTACAACTC TTTAGCTGGC ATAGAAAAGG ATCTCTGTCT TTCTCTGCCG 
CACCTGCAAT TTCTTAATGT GCAACACAAT CAAGTGTATT TGATAAGCGA AAAAAACCTG 
AAAAACTGTT TTCATTTGAC ACAACTTGAC CTGTCTGACA ATAAGCTGAA GCTACAAGGG 
GAGCCATTCT CTCTCTTAAA GAATTTAACA TGGTTGGATG TATCTCGAAA CAAACTGACA 
TCTGCTAAAC TGGGAACAGA ACCTCAGCTG CCAAACCTGG TGACCCTGGT TCTCTCTGGA 
AATAACATTA ACATACTGCA AGAGAATGAC TTCTCATTTC TCAGTAATTC TTCTTCATTT 
CGGGTTCTGA TTCTCTCATC TCTGATTCTT AAAAAGGTGG AGAATGGGTG TTTCAAGGCT 
ATCGATACAC TGTCTGACTT GGTGTTGGAT AGCAGCAAGC TCACCTCTCA GTTTACCACC 
AGTCTTTTTG AAGAACTTGC TGACACAGCC TTGCGAAACC TTTCTCTTAA GAGCACTGAA 
CAGGTGACAC TGTCAAACAC AACTTTCCAA GGTCTAGAGA AGACCAAGAT CACAGTTCTG 
GACCTTAGTG AAAACAGAAT TTCAAAGATT GTAGATGGTG CCTTTCAGTG GTTGCCCCAA 
CTGGAATTTT TGTCTCTGGA GCATAACACA ATTAGACACC TAACTAATGA CTCATTCAGT 
GGACTAGGAA ACCTAAGGCA GCTCAACCTG CGGAAAGCCC TAATTAAGAG TCATGCATCT 
TTACCTGTTA TTGATGACTT TTCCTTCCAA CATCTAGTCC AGTTGGAATA TTTGTGTATG 
GCAAATACTG CCTTCCGAGA GTTAACTGAA CAAATCTTTT CTGGCCTTCG GAACCTGAAG 
ACATTGGATT TGAGTTGGAG CATCACAGGG ATAAAGACAG TCACAAATAA AACCTTTGCT 
GGTCTGCAAG AATCTCCACT CCTTCAGACT CTTAATCTTA CAGGCATGGG CATCAACAAG 
TTGGAGCCTG GTGCCTTCTC AAGTTTAGGA AATCTCAGCA ACCTACTAAT GAGTCGCAAT 
TTTATCAATC AGCAGTTACA AGGGAATGAG TTCAAGGGCC TCTCTAACAT TAAAGAGATT 
GACATGTCTA TAAACCAGCA AAGTATTTCC CTCACCAATG CATCATTCGT TCATGTCTCT 
ACATTGAGGA CCCTAAAGCT AGGTCGTGCT TTAAAAGGGA CTCTAGATAT GGAACCATCT 
CCTTTCAGGC CACTTGTCAA CCTCACAATC CTAGATCTCA GTAATAATAA TATCGCAAAC 
ATAAATGCTG ACTTGTTGGA AGGTCTGTAT AATCTAAAAG TGGTGAAAAT GCAGCACAAC 
AACTTAGCTA GATTGTGGAA AATGGCAAAC CCTGGTGGTC CGGTGCTGTT TCTGAAGGAT 
GCTACAAATC TGTCGTATCT GAATCTGGAT TACAATGGCA TCGATGAGAT TCCACCTAAT 
GCTTTTCGTG GCTTCTCTGA GTTGCATGAA CTAAGTCTTC GTGGCAATCT CTTGGATCAG 
TTGCATGCCT CTGTTTTTGA TGACCTAAAC TCTTTAAAAT ATTTGCATCT TCAAAAGAAC 
CTTATAACAT CTGTCCAAAG GGCTACGTTT GGTGTGCCAC TGTCCAAACT GAAAGAACTT 
AACATGGACC ACAACCCATT TGACTGCACC TGTGAGAGCA TCCTGTGGTT CTCTGAATGG 
CTGAACTCCA CCAACACCAG CGTTCCAGGA TTTCCTCAAA GTTATATCTG CAATACCCCC 
AATGCCTACT TTAATCACTC TGTAATGAAC TTTGATCCTT TGTCCTGCAA GGACATGACA 
CCTTTTAAGG CGCTGTACAT TTTGAGTAGT ACGGCAGTGT TAATGTTGTT GTTTATATCC 
TTCCTGGTGC ACTTCCAGGG ATGGAGAATT CAGTTCTACT GGAATATAAT GGTAAACCGT 
ATGTTAGGGT CGCTGAAGGA TGCAAAAGTT ACTGAAGGTA GATTTCAATT TGATGCCTAT 
ATCATCCACG CAGGTGAAGA TAAATCATGG GTGGAACGAA GCTTGCTCTC TCTGGAGGAC 
AAAGATTTAA ATTTTTTCTA TGAACAGAGA GATTCTACAC CTGGACATTC TCGTCTTGAG 
ACAATTGTTG ATAACATGGT ATACTCCAGG AAGATCATTT TTGTTATAAC AGAAATGCTA 
CTAAAGGATC CATGGTGTAG GCAATTCAAA GCCCATCATG CACTTCATCA TGTAATGGAG 
GATAACCGCG ACTCACTGAT CCTGATTTTC TTGGAAGATG TAACTGATTA CAACCTGAAT 
CGCTCTCTTC ATCTTCGTCG TGGCATGTTG AAGCCTAAAT GTGTCCTCTA CTGGCCTTTA 
CACAAGGAAC GCATCCCAGC ATTTCATCAG AAACTCCGCT CAGCATTAGC CTCTACAAAC 
AAGGTCAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013287.2
CDS	243..2954
Translation	MDLMKLILLPLFYACFSAHCAGTTNARKSACMIKNAKADCSHMNLDAIPTDLPTNITTLDVSHNRLKTLSSLHMYTNLVNIDASYNSLAGIEKDLCLSLPHLQFLNVQHNQVYLISEKNLKNCFHLTQLDLSDNKLKLQGEPFSLLKNLTWLDVSRNKLTSAKLGTEPQLPNLVTLVLSGNNINILQENDFSFLSNSSSFRVLILSSLILKKVENGCFKAIDTLSDLVLDSSKLTSQFTTSLFEELADTALRNLSLKSTEQVTLSNTTFQGLEKTKITVLDLSENRISKIVDGAFQWLPQLEFLSLEHNTIRHLTNDSFSGLGNLRQLNLRKALIKSHASLPVIDDFSFQHLVQLEYLCMANTAFRELTEQIFSGLRNLKTLDLSWSITGIKTVTNKTFAGLQESPLLQTLNLTGMGINKLEPGAFSSLGNLSNLLMSRNFINQQLQGNEFKGLSNIKEIDMSINQQSISLTNASFVHVSTLRTLKLGRALKGTLDMEPSPFRPLVNLTILDLSNNNIANINADLLEGLYNLKVVKMQHNNLARLWKMANPGGPVLFLKDATNLSYLNLDYNGIDEIPPNAFRGFSELHELSLRGNLLDQLHASVFDDLNSLKYLHLQKNLITSVQRATFGVPLSKLKELNMDHNPFDCTCESILWFSEWLNSTNTSVPGFPQSYICNTPNAYFNHSVMNFDPLSCKDMTPFKALYILSSTAVLMLLFISFLVHFQGWRIQFYWNIMVNRMLGSLKDAKVTEGRFQFDAYIIHAGEDKSWVERSLLSLEDKDLNFFYEQRDSTPGHSRLETIVDNMVYSRKIIFVITEMLLKDPWCRQFKAHHALHHVMEDNRDSLILIFLEDVTDYNLNRSLHLRRGMLKPKCVLYWPLHKERIPAFHQKLRSALASTNKVN

Target ORF information:

RefSeq Version	NM_001013269.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio toll-like receptor 3 (tlr3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013269.3

ORF Insert Sequence:

ATGGATCTAA TGAAACTCAT ATTATTGCCC TTGTTCTACG CTTGTTTTAG TGCCCACTGT 
GCAGGCACTA CAAATGCACG CAAGTCAGCT TGCATGATTA AGAATGCCAA AGCAGACTGT 
AGTCATATGA ATCTGGATGC AATTCCAACG GACTTACCTA CAAATATTAC CACACTGGAT 
GTATCTCACA ACAGACTTAA AACTCTGTCT TCTCTACACA TGTACACAAA TCTGGTGAAT 
ATAGATGCGA GCTACAACTC TTTAGCTGGC ATAGAAAAGG ATCTCTGTCT TTCTCTGCCG 
CACCTGCAAT TTCTTAATGT GCAACACAAT CAAGTGTATT TGATAAGCGA AAAAAACCTG 
AAAAACTGTT TTCATTTGAC ACAACTTGAC CTGTCTGACA ATAAGCTGAA GCTACAAGGG 
GAGCCATTCT CTCTCTTAAA GAATTTAACA TGGTTGGATG TATCTCGAAA CAAACTGACA 
TCTGCTAAAC TGGGAACAGA ACCTCAGCTG CCAAACCTGG TGACCCTGGT TCTCTCTGGA 
AATAACATTA ACATACTGCA AGAGAATGAC TTCTCATTTC TCAGTAATTC TTCTTCATTT 
CGGGTTCTGA TTCTCTCATC TCTGATTCTT AAAAAGGTGG AGAATGGGTG TTTCAAGGCT 
ATCGATACAC TGTCTGACTT GGTGTTGGAT AGCAGCAAGC TCACCTCTCA GTTTACCACC 
AGTCTTTTTG AAGAACTTGC TGACACAGCC TTGCGAAACC TTTCTCTTAA GAGCACTGAA 
CAGGTGACAC TGTCAAACAC AACTTTCCAA GGTCTAGAGA AGACCAAGAT CACAGTTCTG 
GACCTTAGTG AAAACAGAAT TTCAAAGATT GTAGATGGTG CCTTTCAGTG GTTGCCCCAA 
CTGGAATTTT TGTCTCTGGA GCATAACACA ATTAGACACC TAACTAATGA CTCATTCAGT 
GGACTAGGAA ACCTAAGGCA GCTCAACCTG CGGAAAGCCC TAATTAAGAG TCATGCATCT 
TTACCTGTTA TTGATGACTT TTCCTTCCAA CATCTAGTCC AGTTGGAATA TTTGTGTATG 
GCAAATACTG CCTTCCGAGA GTTAACTGAA CAAATCTTTT CTGGCCTTCG GAACCTGAAG 
ACATTGGATT TGAGTTGGAG CATCACAGGG ATAAAGACAG TCACAAATAA AACCTTTGCT 
GGTCTGCAAG AATCTCCACT CCTTCAGACT CTTAATCTTA CAGGCATGGG CATCAACAAG 
TTGGAGCCTG GTGCCTTCTC AAGTTTAGGA AATCTCAGCA ACCTACTAAT GAGTCGCAAT 
TTTATCAATC AGCAGTTACA AGGGAATGAG TTCAAGGGCC TCTCTAACAT TAAAGAGATT 
GACATGTCTA TAAACCAGCA AAGTATTTCC CTCACCAATG CATCATTCGT TCATGTCTCT 
ACATTGAGGA CCCTAAAGCT AGGTCGTGCT TTAAAAGGGA CTCTAGATAT GGAACCATCT 
CCTTTCAGGC CACTTGTCAA CCTCACAATC CTAGATCTCA GTAATAATAA TATCGCAAAC 
ATAAATGCTG ACTTGTTGGA AGGTCTGTAT AATCTAAAAG TGGTGAAAAT GCAGCACAAC 
AACTTAGCTA GATTGTGGAA AATGGCAAAC CCTGGTGGTC CGGTGCTGTT TCTGAAGGAT 
GCTACAAATC TGTCGTATCT GAATCTGGAT TACAATGGCA TCGATGAGAT TCCACCTAAT 
GCTTTTCGTG GCTTCTCTGA GTTGCATGAA CTAAGTCTTC GTGGCAATCT CTTGGATCAG 
TTGCATGCCT CTGTTTTTGA TGACCTAAAC TCTTTAAAAT ATTTGCATCT TCAAAAGAAC 
CTTATAACAT CTGTCCAAAG GGCTACGTTT GGTGTGCCAC TGTCCAAACT GAAAGAACTT 
AACATGGACC ACAACCCATT TGACTGCACC TGTGAGAGCA TCCTGTGGTT CTCTGAATGG 
CTGAACTCCA CCAACACCAG CGTTCCAGGA TTTCCTCAAA GTTATATCTG CAATACCCCC 
AATGCCTACT TTAATCACTC TGTAATGAAC TTTGATCCTT TGTCCTGCAA GGACATGACA 
CCTTTTAAGG CGCTGTACAT TTTGAGTAGT ACGGCAGTGT TAATGTTGTT GTTTATATCC 
TTCCTGGTGC ACTTCCAGGG ATGGAGAATT CAGTTCTACT GGAATATAAT GGTAAACCGT 
ATGTTAGGGT CGCTGAAGGA TGCAAAAGTT ACTGAAGGTA GATTTCAATT TGATGCCTAT 
ATCATCCACG CAGGTGAAGA TAAATCATGG GTGGAACGAA GCTTGCTCTC TCTGGAGGAC 
AAAGATTTAA ATTTTTTCTA TGAACAGAGA GATTCTACAC CTGGACATTC TCGTCTTGAG 
ACAATTGTTG ATAACATGGT ATACTCCAGG AAGATCATTT TTGTTATAAC AGAAATGCTA 
CTAAAGGATC CATGGTGTAG GCAATTCAAA GCCCATCATG CACTTCATCA TGTAATGGAG 
GATAACCGCG ACTCACTGAT CCTGATTTTC TTGGAAGATG TAACTGATTA CAACCTGAAT 
CGCTCTCTTC ATCTTCGTCG TGGCATGTTG AAGCCTAAAT GTGTCCTCTA CTGGCCTTTA 
CACAAGGAAC GCATCCCAGC ATTTCATCAG AAACTCCGCT CAGCATTAGC CTCTACAAAC 
AAGGTCAACT AG