tjp1a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009303250.3	XP_009301525.1	tight junction protein ZO-1 isoform X1
XM_005168936.4	XP_005168993.1	tight junction protein ZO-1 isoform X8
XM_009303256.3	XP_009301531.1	tight junction protein ZO-1 isoform X10
XM_009303258.3	XP_009301533.1	tight junction protein ZO-1 isoform X13
XM_021477863.1	XP_021333538.1	tight junction protein ZO-1 isoform X14
XM_009303250.2	XP_009301525.1	tight junction protein ZO-1 isoform X1
XM_009303251.2	XP_009301526.1	tight junction protein ZO-1 isoform X2
XM_009303252.2	XP_009301527.1	tight junction protein ZO-1 isoform X3
XM_005168933.3	XP_005168990.1	tight junction protein ZO-1 isoform X4
XM_009303255.2	XP_009301530.1	tight junction protein ZO-1 isoform X8
XM_009303257.2	XP_009301532.1	tight junction protein ZO-1 isoform X10
XM_009303255.3	XP_009301530.1	tight junction protein ZO-1 isoform X9
XM_009303258.2	XP_009301533.1	tight junction protein ZO-1 isoform X11
XM_009303253.3	XP_009301528.1	tight junction protein ZO-1 isoform X5
XM_009303256.2	XP_009301531.1	tight junction protein ZO-1 isoform X9
XM_009303251.3	XP_009301526.1	tight junction protein ZO-1 isoform X2
XM_009303252.3	XP_009301527.1	tight junction protein ZO-1 isoform X3
XM_021477860.1	XP_021333535.1	tight junction protein ZO-1 isoform X6
XM_009303253.2	XP_009301528.1	tight junction protein ZO-1 isoform X5
XM_009303254.2	XP_009301529.1	tight junction protein ZO-1 isoform X6
XM_005168936.3	XP_005168993.1	tight junction protein ZO-1 isoform X7
XM_005168933.4	XP_005168990.1	tight junction protein ZO-1 isoform X4
XM_009303254.3	XP_009301529.1	tight junction protein ZO-1 isoform X7
XM_009303257.3	XP_009301532.1	tight junction protein ZO-1 isoform X11
XM_021477862.1	XP_021333537.1	tight junction protein ZO-1 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143461	XM_021477860.1 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143462	XM_021477862.1 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa143463	XM_021477863.1 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa13132	XM_005168933.4 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13135	XM_005168936.4 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40325	XM_009303250.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40326	XM_009303251.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40327	XM_009303252.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40328	XM_009303253.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40329	XM_009303254.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40330	XM_009303255.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40331	XM_009303256.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40332	XM_009303257.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40333	XM_009303258.3 Latest version!	Danio rerio tight junction protein 1a (tjp1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa40325	XM_009303250.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40330	XM_009303255.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40333	XM_009303258.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40331	XM_009303256.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40326	XM_009303251.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40327	XM_009303252.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40328	XM_009303253.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13135	XM_005168936.3	Danio rerio tight junction protein 1a (tjp1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13132	XM_005168933.3	Danio rerio tight junction protein 1a (tjp1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40329	XM_009303254.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40332	XM_009303257.2	Danio rerio tight junction protein 1a (tjp1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

tjp1a ( XM_021477860.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333535.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa143461
Clone ID Related Accession (Same CDS sequence)	XM_021477860.1
Accession Version	XM_021477860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGACA CAGCTCCCAG ATGGAAGGGA AAAGAGTGAA 
CCTAGAGATG CCGGCACTGA CCAGCGCAGC TCGGGGATCA TACGCCTTCA CACTATCAAA 
CAGATAATCG ACCGGGATAA GCATGCAGTG CTGGACATTA CTCCTAACGC AGTGGATCGG 
CTGAACTATG CTCAGTGGTA TCCCATCGTA GTGTTCTTAA ATCCTGACAG CAAGCAGGGT 
GTGAAGAACA TGAGGACCAG ACTGTGTTCC GAGTCCAGGA AAAGTGCCAG AAAACTCCAC 
GAGCGCGCAC TCAAACTGAG GAAGAACAAC CATCATCTCT TCACAGCCAC TATTAACATG 
AATAACATGA ATGATGGCTG GTATGGAGCA CTGAAGGACA CCATTCAGCA GCAGCAGAAC 
CAGCTGGTGT GGGTCTCAGA GGGCAAGGCG GACGGTTCAC ATGATGATGA CCTGGACCTT 
CACGATGACC GTCTGTCCTA TTTATCGGCC CCAGGCAGTG AGTACAGTAT GTACAGCACG 
GACAGCCGCC ACACCTCAGA TTACGAGGAC ACGGACACCG AGGGTGGCGC CTACACTGAC 
CAGGAACTGG ACGAAACCCT GAACGATGAG GTCGGACCAC CCAGCGAACC AGCCATCACT 
CGCTCCTCAG AACCTGTCCG CGAAGACCCG CCCGTCATCC AGGAGCCCCC CGGATATGGA 
GGATATCAGC CCGAACCCAT CAACCGCATC GACCCCGCGG GGTTCAAGGC CCCTGCACCT 
CAGCAGAAAG CAGAGGCTGC TCTCCCCTCG CAGCCTGAGC CACTGGCAGA GACAGCGCCC 
CCTGCTGTTC ATGTAAATGT AGGGGCCCCG AGTGCCAAGG ATCTCACTCA GGGGGAAATC 
AGCCCCAAGG CTGGCTCTCT CTTCCCACCT GCCCCTGAGC TTATCCAACC CCCGTCTCCT 
GACCCCAACC AATCCGGACA GCCCGGGTCA GAGTCCAAGA TGTACAAGAA GGATATCTAC 
CGACCCAACG AACCGGTGCA GATAAGCCAA AACCCTATGC AGCCCAACTA CTACCAGGAA 
GACAAGCCAT ACAGAGATTA TGACCACCAG CCCTACCGCT ATGATGGAGG CTACATGGAA 
CCAAAGGCTC GTAACTTTGA CCCACATTTT GAGGACAGCG TGCCTCCATA TAATGATCAA 
TGGGGTCAGT ATGACCACAC CTCTGGGTAT GAACCTCAAA CGCTCTACGA GGATGGTCTG 
AACCGCTTGT ATGGTCACCC TCAGTCACGA AACGACACCG GCTACGACAC AGGTCGACCT 
CGATATGGCA AACCAACCTC TGGACCAATC CGACACGATG AACCACCACC ATCACGCCCA 
CAATATGACA CTCAACAAAC CTCTCGTTCC CCAGAACCGC CCAAACAACA ATACTACGAC 
CCTTCCCAAA GGCCTTCCTA CACTCAAGCT CCACAAAAAG GCTATATGAA CTCTGAAGCA 
CCGGTAACGC CACCCAAACC TGAGTCGCTC TCACCGCCTT TGGAGCCTGC TCTTCCTCCT 
CCACCTCCTC CAGCCGAAGC AGAGGATGAT CCCGCCATGA AGCCCCAGTC GGTGCTCACC 
AGGGTGAAGA TGTTCGAGAA CAAGCGATCG GTGTCTGTAG ACAGAGCCAG AGAGTCTGCA 
GACTCTGGTA TTCGGCCTGT GGACATGGTG CCTAAACCTG GCGCTGCCTC CGTGCCCAAA 
GCCAATTCTT TAAGTAACCT GGATCAAGAG AAGACCTTTA GAACTCCAGA GCCCCAGCAG 
CCACAGCCTA AAGTAGATGA TATCATTCGT TCAAATAACA ATGACCCTGA TGAGGATGAA 
GAATATTACA GGAAACAACT GTCCTACTTC GACCGCAGGA GCTTCGACAA ACCCTCCACA 
CAGCTGACAC CTGCCATCAA ACCAGCCCAA CCCCAAACTC AACCAGCACA CTATCCCAGA 
GCAGAGTCAG TGGAGAAAAT CAACCCAGCT GCTCCATCAA ACCCAGCTGT CCCACCACCA 
ACCCTGCCTA AACCTGCAGC AACCCCACCT CTCGACCCTC ACGGATCTCC CAAAGTAAAG 
CCTCCTAGTC ATGAAGACAC AGTCCAGAGC ACTTACCTGC CTCACAAAAG CTTCCCAGAG 
AAATCCCCCG TCAACGGCAC CGGCGAACCT CCAAAGACCA ACAGCACTGC CACGTCCAGC 
TACAACCGTT TTGTGCCCAA GCCGTACACC ACCTCCGCAA GACCCTTCGA GCGCAAGTTT 
GACAGTCCCA AGTTCAATCA CAATCTCCTC CCTAATGACT CCCAAAACAA GACGGATAAT 
GTGAAAGCTC CCATCACCGC TAAACCACAG AGCTCACCCC AGCCGGCAGA CCACGACAGC 
GGCCTGGACA CCTTCACACG CACCATGGAC CACAAACCCA AATACCAGCA GAACAACATC 
AACGCCGTAC CCAAGGCAAT ACCTGTCAGC CCGAGTGCTT TGGAGGATGA TGACGATGAT 
GACAGCCACA CGGTGGTGGC GACAGCGCGG GGTATTTTCA ACTCTAATGG TGGGGTCCTG 
AGCTCCATTG AGACAGGAGT AAGCATTATT ATCCCACAGG GAGCCATCCC TGATGGCGTG 
GAGCAAGAGA TCTACTTTAA AGTGTGTCGG GATAACAGCA TCCTGCCACC TCTGGACAAA 
GAGAAAGGAG AAACTCTGTT GAGTCCTCTG GTGATGTGTG GTCCTCACGG TCTGAAATTC 
CTGAAGCCAG TGGAGCTCCG CTTACCTCAC TGTGCGTCTA TGACCCCTGA TGGTTGGTCT 
TTTGCTCTAA AATCCTCCGA CTCCTCGTCG GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333535.1
CDS	384..5549
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021477860.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477860.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGACA CAGCTCCCAG ATGGAAGGGA AAAGAGTGAA 
CCTAGAGATG CCGGCACTGA CCAGCGCAGC TCGGGGATCA TACGCCTTCA CACTATCAAA 
CAGATAATCG ACCGGGATAA GCATGCAGTG CTGGACATTA CTCCTAACGC AGTGGATCGG 
CTGAACTATG CTCAGTGGTA TCCCATCGTA GTGTTCTTAA ATCCTGACAG CAAGCAGGGT 
GTGAAGAACA TGAGGACCAG ACTGTGTTCC GAGTCCAGGA AAAGTGCCAG AAAACTCCAC 
GAGCGCGCAC TCAAACTGAG GAAGAACAAC CATCATCTCT TCACAGCCAC TATTAACATG 
AATAACATGA ATGATGGCTG GTATGGAGCA CTGAAGGACA CCATTCAGCA GCAGCAGAAC 
CAGCTGGTGT GGGTCTCAGA GGGCAAGGCG GACGGTTCAC ATGATGATGA CCTGGACCTT 
CACGATGACC GTCTGTCCTA TTTATCGGCC CCAGGCAGTG AGTACAGTAT GTACAGCACG 
GACAGCCGCC ACACCTCAGA TTACGAGGAC ACGGACACCG AGGGTGGCGC CTACACTGAC 
CAGGAACTGG ACGAAACCCT GAACGATGAG GTCGGACCAC CCAGCGAACC AGCCATCACT 
CGCTCCTCAG AACCTGTCCG CGAAGACCCG CCCGTCATCC AGGAGCCCCC CGGATATGGA 
GGATATCAGC CCGAACCCAT CAACCGCATC GACCCCGCGG GGTTCAAGGC CCCTGCACCT 
CAGCAGAAAG CAGAGGCTGC TCTCCCCTCG CAGCCTGAGC CACTGGCAGA GACAGCGCCC 
CCTGCTGTTC ATGTAAATGT AGGGGCCCCG AGTGCCAAGG ATCTCACTCA GGGGGAAATC 
AGCCCCAAGG CTGGCTCTCT CTTCCCACCT GCCCCTGAGC TTATCCAACC CCCGTCTCCT 
GACCCCAACC AATCCGGACA GCCCGGGTCA GAGTCCAAGA TGTACAAGAA GGATATCTAC 
CGACCCAACG AACCGGTGCA GATAAGCCAA AACCCTATGC AGCCCAACTA CTACCAGGAA 
GACAAGCCAT ACAGAGATTA TGACCACCAG CCCTACCGCT ATGATGGAGG CTACATGGAA 
CCAAAGGCTC GTAACTTTGA CCCACATTTT GAGGACAGCG TGCCTCCATA TAATGATCAA 
TGGGGTCAGT ATGACCACAC CTCTGGGTAT GAACCTCAAA CGCTCTACGA GGATGGTCTG 
AACCGCTTGT ATGGTCACCC TCAGTCACGA AACGACACCG GCTACGACAC AGGTCGACCT 
CGATATGGCA AACCAACCTC TGGACCAATC CGACACGATG AACCACCACC ATCACGCCCA 
CAATATGACA CTCAACAAAC CTCTCGTTCC CCAGAACCGC CCAAACAACA ATACTACGAC 
CCTTCCCAAA GGCCTTCCTA CACTCAAGCT CCACAAAAAG GCTATATGAA CTCTGAAGCA 
CCGGTAACGC CACCCAAACC TGAGTCGCTC TCACCGCCTT TGGAGCCTGC TCTTCCTCCT 
CCACCTCCTC CAGCCGAAGC AGAGGATGAT CCCGCCATGA AGCCCCAGTC GGTGCTCACC 
AGGGTGAAGA TGTTCGAGAA CAAGCGATCG GTGTCTGTAG ACAGAGCCAG AGAGTCTGCA 
GACTCTGGTA TTCGGCCTGT GGACATGGTG CCTAAACCTG GCGCTGCCTC CGTGCCCAAA 
GCCAATTCTT TAAGTAACCT GGATCAAGAG AAGACCTTTA GAACTCCAGA GCCCCAGCAG 
CCACAGCCTA AAGTAGATGA TATCATTCGT TCAAATAACA ATGACCCTGA TGAGGATGAA 
GAATATTACA GGAAACAACT GTCCTACTTC GACCGCAGGA GCTTCGACAA ACCCTCCACA 
CAGCTGACAC CTGCCATCAA ACCAGCCCAA CCCCAAACTC AACCAGCACA CTATCCCAGA 
GCAGAGTCAG TGGAGAAAAT CAACCCAGCT GCTCCATCAA ACCCAGCTGT CCCACCACCA 
ACCCTGCCTA AACCTGCAGC AACCCCACCT CTCGACCCTC ACGGATCTCC CAAAGTAAAG 
CCTCCTAGTC ATGAAGACAC AGTCCAGAGC ACTTACCTGC CTCACAAAAG CTTCCCAGAG 
AAATCCCCCG TCAACGGCAC CGGCGAACCT CCAAAGACCA ACAGCACTGC CACGTCCAGC 
TACAACCGTT TTGTGCCCAA GCCGTACACC ACCTCCGCAA GACCCTTCGA GCGCAAGTTT 
GACAGTCCCA AGTTCAATCA CAATCTCCTC CCTAATGACT CCCAAAACAA GACGGATAAT 
GTGAAAGCTC CCATCACCGC TAAACCACAG AGCTCACCCC AGCCGGCAGA CCACGACAGC 
GGCCTGGACA CCTTCACACG CACCATGGAC CACAAACCCA AATACCAGCA GAACAACATC 
AACGCCGTAC CCAAGGCAAT ACCTGTCAGC CCGAGTGCTT TGGAGGATGA TGACGATGAT 
GACAGCCACA CGGTGGTGGC GACAGCGCGG GGTATTTTCA ACTCTAATGG TGGGGTCCTG 
AGCTCCATTG AGACAGGAGT AAGCATTATT ATCCCACAGG GAGCCATCCC TGATGGCGTG 
GAGCAAGAGA TCTACTTTAA AGTGTGTCGG GATAACAGCA TCCTGCCACC TCTGGACAAA 
GAGAAAGGAG AAACTCTGTT GAGTCCTCTG GTGATGTGTG GTCCTCACGG TCTGAAATTC 
CTGAAGCCAG TGGAGCTCCG CTTACCTCAC TGTGCGTCTA TGACCCCTGA TGGTTGGTCT 
TTTGCTCTAA AATCCTCCGA CTCCTCGTCG GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_021477862.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333537.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa143462
Clone ID Related Accession (Same CDS sequence)	XM_021477862.1
Accession Version	XM_021477862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA TGTACAAGAA GGATATCTAC 
CGACCCAACG AACCGGTGCA GATAAGCCAA AACCCTATGC AGCCCAACTA CTACCAGGAA 
GACAAGCCAT ACAGAGATTA TGACCACCAG CCCTACCGCT ATGATGGAGG CTACATGGAA 
CCAAAGGCTC GTAACTTTGA CCCACATTTT GAGGACAGCG TGCCTCCATA TAATGATCAA 
TGGGGTCAGT ATGACCACAC CTCTGGGTAT GAACCTCAAA CGCTCTACGA GGATGGTCTG 
AACCGCTTGT ATGGTCACCC TCAGTCACGA AACGACACCG GCTACGACAC AGGTCGACCT 
CGATATGGCA AACCAACCTC TGGACCAATC CGACACGATG AACCACCACC ATCACGCCCA 
CAATATGACA CTCAACAAAC CTCTCGTTCC CCAGAACCGC CCAAACAACA ATACTACGAC 
CCTTCCCAAA GGCCTTCCTA CACTCAAGCT CCACAAAAAG GCTATATGAA CTCTGAAGCA 
CCGGTAACGC CACCCAAACC TGAGTCGCTC TCACCGCCTT TGGAGCCTGC TCTTCCTCCT 
CCACCTCCTC CAGCCGAAGC AGAGGATGAT CCCGCCATGA AGCCCCAGTC GGTGCTCACC 
AGGGTGAAGA TGTTCGAGAA CAAGCGATCG GTGTCTGTAG ACAGAGCCAG AGAGTCTGCA 
GACTCTGGTA TTCGGCCTGT GGACATGGTG CCTAAACCTG GCGCTGCCTC CGTGCCCAAA 
GCCAATTCTT TAAGTAACCT GGATCAAGAG AAGACCTTTA GAACTCCAGA GCCCCAGCAG 
CCACAGCCTA AAGTAGATGA TATCATTCGT TCAAATAACA ATGACCCTGA TGAGGATGAA 
GAATATTACA GGAAACAACT GTCCTACTTC GACCGCAGGA GCTTCGACAA ACCCTCCACA 
CAGCTGACAC CTGCCATCAA ACCAGCCCAA CCCCAAACTC AACCAGCACA CTATCCCAGA 
GCAGAGTCAG TGGAGAAAAT CAACCCAGCT GCTCCATCAA ACCCAGCTGT CCCACCACCA 
ACCCTGCCTA AACCTGCAGC AACCCCACCT CTCGACCCTC ACGGATCTCC CAAAGTAAAG 
CCTCCTAGTC ATGAAGACAC AGTCCAGAGC ACTTACCTGC CTCACAAAAG CTTCCCAGAG 
AAATCCCCCG TCAACGGCAC CGGCGAACCT CCAAAGACCA ACAGCACTGC CACGTCCAGC 
TACAACCGTT TTGTGCCCAA GCCGTACACC ACCTCCGCAA GACCCTTCGA GCGCAAGTTT 
GACAGTCCCA AGTTCAATCA CAATCTCCTC CCTAATGACT CCCAAAACAA GACGGATAAT 
GTGAAAGCTC CCATCACCGC TAAACCACAG AGCTCACCCC AGCCGGCAGA CCACGACAGC 
GGCCTGGACA CCTTCACACG CACCATGGAC CACAAACCCA AATACCAGCA GAACAACATC 
AACGCCGTAC CCAAGGCAAT ACCTGTCAGC CCGAGTGCTT TGGAGGATGA TGACGATGAT 
GACAGCCACA CGGTGGTGGC GACAGCGCGG GGTATTTTCA ACTCTAATGG TGGGGTCCTG 
AGCTCCATTG AGACAGGAGT AAGCATTATT ATCCCACAGG GAGCCATCCC TGATGGCGTG 
GAGCAAGAGA TCTACTTTAA AGTGTGTCGG GATAACAGCA TCCTGCCACC TCTGGACAAA 
GAGAAAGGAG AAACTCTGTT GAGTCCTCTG GTGATGTGTG GTCCTCACGG TCTGAAATTC 
CTGAAGCCAG TGGAGCTCCG CTTACCTCAC TGTGCGTCTA TGACCCCTGA TGGTTGGTCT 
TTTGCTCTAA AATCCTCCGA CTCCTCGTCG GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333537.1
CDS	383..5308
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021477862.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477862.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA TGTACAAGAA GGATATCTAC 
CGACCCAACG AACCGGTGCA GATAAGCCAA AACCCTATGC AGCCCAACTA CTACCAGGAA 
GACAAGCCAT ACAGAGATTA TGACCACCAG CCCTACCGCT ATGATGGAGG CTACATGGAA 
CCAAAGGCTC GTAACTTTGA CCCACATTTT GAGGACAGCG TGCCTCCATA TAATGATCAA 
TGGGGTCAGT ATGACCACAC CTCTGGGTAT GAACCTCAAA CGCTCTACGA GGATGGTCTG 
AACCGCTTGT ATGGTCACCC TCAGTCACGA AACGACACCG GCTACGACAC AGGTCGACCT 
CGATATGGCA AACCAACCTC TGGACCAATC CGACACGATG AACCACCACC ATCACGCCCA 
CAATATGACA CTCAACAAAC CTCTCGTTCC CCAGAACCGC CCAAACAACA ATACTACGAC 
CCTTCCCAAA GGCCTTCCTA CACTCAAGCT CCACAAAAAG GCTATATGAA CTCTGAAGCA 
CCGGTAACGC CACCCAAACC TGAGTCGCTC TCACCGCCTT TGGAGCCTGC TCTTCCTCCT 
CCACCTCCTC CAGCCGAAGC AGAGGATGAT CCCGCCATGA AGCCCCAGTC GGTGCTCACC 
AGGGTGAAGA TGTTCGAGAA CAAGCGATCG GTGTCTGTAG ACAGAGCCAG AGAGTCTGCA 
GACTCTGGTA TTCGGCCTGT GGACATGGTG CCTAAACCTG GCGCTGCCTC CGTGCCCAAA 
GCCAATTCTT TAAGTAACCT GGATCAAGAG AAGACCTTTA GAACTCCAGA GCCCCAGCAG 
CCACAGCCTA AAGTAGATGA TATCATTCGT TCAAATAACA ATGACCCTGA TGAGGATGAA 
GAATATTACA GGAAACAACT GTCCTACTTC GACCGCAGGA GCTTCGACAA ACCCTCCACA 
CAGCTGACAC CTGCCATCAA ACCAGCCCAA CCCCAAACTC AACCAGCACA CTATCCCAGA 
GCAGAGTCAG TGGAGAAAAT CAACCCAGCT GCTCCATCAA ACCCAGCTGT CCCACCACCA 
ACCCTGCCTA AACCTGCAGC AACCCCACCT CTCGACCCTC ACGGATCTCC CAAAGTAAAG 
CCTCCTAGTC ATGAAGACAC AGTCCAGAGC ACTTACCTGC CTCACAAAAG CTTCCCAGAG 
AAATCCCCCG TCAACGGCAC CGGCGAACCT CCAAAGACCA ACAGCACTGC CACGTCCAGC 
TACAACCGTT TTGTGCCCAA GCCGTACACC ACCTCCGCAA GACCCTTCGA GCGCAAGTTT 
GACAGTCCCA AGTTCAATCA CAATCTCCTC CCTAATGACT CCCAAAACAA GACGGATAAT 
GTGAAAGCTC CCATCACCGC TAAACCACAG AGCTCACCCC AGCCGGCAGA CCACGACAGC 
GGCCTGGACA CCTTCACACG CACCATGGAC CACAAACCCA AATACCAGCA GAACAACATC 
AACGCCGTAC CCAAGGCAAT ACCTGTCAGC CCGAGTGCTT TGGAGGATGA TGACGATGAT 
GACAGCCACA CGGTGGTGGC GACAGCGCGG GGTATTTTCA ACTCTAATGG TGGGGTCCTG 
AGCTCCATTG AGACAGGAGT AAGCATTATT ATCCCACAGG GAGCCATCCC TGATGGCGTG 
GAGCAAGAGA TCTACTTTAA AGTGTGTCGG GATAACAGCA TCCTGCCACC TCTGGACAAA 
GAGAAAGGAG AAACTCTGTT GAGTCCTCTG GTGATGTGTG GTCCTCACGG TCTGAAATTC 
CTGAAGCCAG TGGAGCTCCG CTTACCTCAC TGTGCGTCTA TGACCCCTGA TGGTTGGTCT 
TTTGCTCTAA AATCCTCCGA CTCCTCGTCG GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_021477863.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333538.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa143463
Clone ID Related Accession (Same CDS sequence)	XM_021477863.1
Accession Version	XM_021477863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA TGTACAAGAA GGATATCTAC 
CGACCCAACG AACCGGTGCA GATAAGCCAA AACCCTATGC AGCCCAACTA CTACCAGGAA 
GACAAGCCAT ACAGAGATTA TGACCACCAG CCCTACCGCT ATGATGGAGG CTACATGGAA 
CCAAAGGCTC GTAACTTTGA CCCACATTTT GAGGACAGCG TGCCTCCATA TAATGATCAA 
TGGGGTCAGT ATGACCACAC CTCTGGGTAT GAACCTCAAA CGCTCTACGA GGATGGTCTG 
AACCGCTTGT ATGGTCACCC TCAGTCACGA AACGACACCG GCTACGACAC AGGTCGACCT 
CGATATGGCA AACCAACCTC TGGACCAATC CGACACGATG AACCACCACC ATCACGCCCA 
CAATATGACA CTCAACAAAC CTCTCGTTCC CCAGAACCGC CCAAACAACA ATACTACGAC 
CCTTCCCAAA GGCCTTCCTA CACTCAAGCT CCACAAAAAG GCTATATGAA CTCTGAAGCA 
CCGGTAACGC CACCCAAACC TGAGTCGCTC TCACCGCCTT TGGAGCCTGC TCTTCCTCCT 
CCACCTCCTC CAGCCGAAGC AGAGGATGAT CCCGCCATGA AGCCCCAGTC GGTGCTCACC 
AGGGTGAAGA TGTTCGAGAA CAAGCGATCG GTGTCTGTAG ACAGAGCCAG AGAGTCTGCA 
GACTCTGGTA TTCGGCCTGT GGACATGGTG CCTAAACCTG GCGCTGCCTC CGTGCCCAAA 
GCCAATTCTT TAAGTAACCT GGATCAAGAG AAGACCTTTA GAACTCCAGA GCCCCAGCAG 
CCACAGCCTA AAGTAGATGA TATCATTCGT TCAAATAACA ATGACCCTGA TGAGGATGAA 
GAATATTACA GGAAACAACT GTCCTACTTC GACCGCAGGA GCTTCGACAA ACCCTCCACA 
CAGCTGACAC CTGCCATCAA ACCAGCCCAA CCCCAAACTC AACCAGCACA CTATCCCAGA 
GCAGAGTCAG TGGAGAAAAT CAACCCAGCT GCTCCATCAA ACCCAGCTGT CCCACCACCA 
ACCCTGCCTA AACCTGCAGC AACCCCACCT CTCGACCCTC ACGGATCTCC CAAAGTAAAG 
CCTCCTAGTC ATGAAGACAC AGTCCAGAGC ACTTACCTGC CTCACAAAAG CTTCCCAGAG 
AAATCCCCCG TCAACGGCAC CGGCGAACCT CCAAAGACCA ACAGCACTGC CACGTCCAGC 
TACAACCGTT TTGTGCCCAA GCCGTACACC ACCTCCGCAA GACCCTTCGA GCGCAAGTTT 
GACAGTCCCA AGTTCAATCA CAATCTCCTC CCTAATGACT CCCAAAACAA GACGGATAAT 
GTGAAAGCTC CCATCACCGC TAAACCACAG AGCTCACCCC AGCCGGCAGA CCACGACAGC 
GGCCTGGACA CCTTCACACG CACCATGGAC CACAAACCCA AATACCAGCA GAACAACATC 
AACGCCGTAC CCAAGGCAAT ACCTGTCAGC CCGAGTGCTT TGGAGGATGA TGACGATGAT 
GACAGCCACA CGGTGGTGGC GACAGCGCGG GGTATTTTCA ACTCTAATGG TGGGGTCCTG 
AGCTCCATTG AGACAGGAGT AAGCATTATT ATCCCACAGG GAGCCATCCC TGATGGCGTG 
GAGCAAGAGA TCTACTTTAA AGTGTGTCGG GATAACAGCA TCCTGCCACC TCTGGACAAA 
GAGAAAGGAG AAACTCTGTT GAGTCCTCTG GTGATGTGTG GTCCTCACGG TCTGAAATTC 
CTGAAGCCAG TGGAGCTCCG CTTACCTCAC TGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333538.1
CDS	382..5247
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021477863.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477863.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA TGTACAAGAA GGATATCTAC 
CGACCCAACG AACCGGTGCA GATAAGCCAA AACCCTATGC AGCCCAACTA CTACCAGGAA 
GACAAGCCAT ACAGAGATTA TGACCACCAG CCCTACCGCT ATGATGGAGG CTACATGGAA 
CCAAAGGCTC GTAACTTTGA CCCACATTTT GAGGACAGCG TGCCTCCATA TAATGATCAA 
TGGGGTCAGT ATGACCACAC CTCTGGGTAT GAACCTCAAA CGCTCTACGA GGATGGTCTG 
AACCGCTTGT ATGGTCACCC TCAGTCACGA AACGACACCG GCTACGACAC AGGTCGACCT 
CGATATGGCA AACCAACCTC TGGACCAATC CGACACGATG AACCACCACC ATCACGCCCA 
CAATATGACA CTCAACAAAC CTCTCGTTCC CCAGAACCGC CCAAACAACA ATACTACGAC 
CCTTCCCAAA GGCCTTCCTA CACTCAAGCT CCACAAAAAG GCTATATGAA CTCTGAAGCA 
CCGGTAACGC CACCCAAACC TGAGTCGCTC TCACCGCCTT TGGAGCCTGC TCTTCCTCCT 
CCACCTCCTC CAGCCGAAGC AGAGGATGAT CCCGCCATGA AGCCCCAGTC GGTGCTCACC 
AGGGTGAAGA TGTTCGAGAA CAAGCGATCG GTGTCTGTAG ACAGAGCCAG AGAGTCTGCA 
GACTCTGGTA TTCGGCCTGT GGACATGGTG CCTAAACCTG GCGCTGCCTC CGTGCCCAAA 
GCCAATTCTT TAAGTAACCT GGATCAAGAG AAGACCTTTA GAACTCCAGA GCCCCAGCAG 
CCACAGCCTA AAGTAGATGA TATCATTCGT TCAAATAACA ATGACCCTGA TGAGGATGAA 
GAATATTACA GGAAACAACT GTCCTACTTC GACCGCAGGA GCTTCGACAA ACCCTCCACA 
CAGCTGACAC CTGCCATCAA ACCAGCCCAA CCCCAAACTC AACCAGCACA CTATCCCAGA 
GCAGAGTCAG TGGAGAAAAT CAACCCAGCT GCTCCATCAA ACCCAGCTGT CCCACCACCA 
ACCCTGCCTA AACCTGCAGC AACCCCACCT CTCGACCCTC ACGGATCTCC CAAAGTAAAG 
CCTCCTAGTC ATGAAGACAC AGTCCAGAGC ACTTACCTGC CTCACAAAAG CTTCCCAGAG 
AAATCCCCCG TCAACGGCAC CGGCGAACCT CCAAAGACCA ACAGCACTGC CACGTCCAGC 
TACAACCGTT TTGTGCCCAA GCCGTACACC ACCTCCGCAA GACCCTTCGA GCGCAAGTTT 
GACAGTCCCA AGTTCAATCA CAATCTCCTC CCTAATGACT CCCAAAACAA GACGGATAAT 
GTGAAAGCTC CCATCACCGC TAAACCACAG AGCTCACCCC AGCCGGCAGA CCACGACAGC 
GGCCTGGACA CCTTCACACG CACCATGGAC CACAAACCCA AATACCAGCA GAACAACATC 
AACGCCGTAC CCAAGGCAAT ACCTGTCAGC CCGAGTGCTT TGGAGGATGA TGACGATGAT 
GACAGCCACA CGGTGGTGGC GACAGCGCGG GGTATTTTCA ACTCTAATGG TGGGGTCCTG 
AGCTCCATTG AGACAGGAGT AAGCATTATT ATCCCACAGG GAGCCATCCC TGATGGCGTG 
GAGCAAGAGA TCTACTTTAA AGTGTGTCGG GATAACAGCA TCCTGCCACC TCTGGACAAA 
GAGAAAGGAG AAACTCTGTT GAGTCCTCTG GTGATGTGTG GTCCTCACGG TCTGAAATTC 
CTGAAGCCAG TGGAGCTCCG CTTACCTCAC TGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_005168933.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168990.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X4, mRNA.

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CloneID	ODa13132
Clone ID Related Accession (Same CDS sequence)	XM_005168933.3 , XM_005168933.4
Accession Version	XM_005168933.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGCAGGCC CAGTATGAAG 
CTGGTGAAGT TTAAGAAAGG GGAAAGTGTC GGATTGAGGC TGGCTGGAGG AAACGATGTG 
GGGATCTTTG TGGCTGGAGT TCTGGAGGAC AGTCCTGCTG CTAAGGAAGG CCTGGAGGAG 
GGAGACCAGA TACTCAGGGT TAACAATGTT GACTTTGCAA ACATTATTCG TGAGGAAGCA 
GTTCTGTTCC TGCTAGATCT GCCCAGAGGT GAGGAGGTCA CCATCCTTGC GCAGAAGAAG 
AAAGATGTGT ACCGGCGAAT AGTGGAGTCT GATGTGGGCG ACTCCTTCTA CATCAGGACA 
CACTTTGAGT ACGAGAAAGA GTCTCCTTAT GGGCTGAGCT TTAATAAAGG AGAGGTGTTC 
AGAGTGGTGG ACACTCTCTA CAATGGCAAA CTGGGCTCCT GGCTGGCTAT CCGCATCGGG 
AAGAACCACC AGGAGGTGGA AAGAGGCATC ATTCCCAACA AGAACAGGGC GGAACAGTTA 
TCAAGCGTAC AGTACACACT CCCCAAGACT GCAGGTGGTG ATCGTGCTGA TTTCTGGAGG 
TTCAGAGGCC TGCGCAGTTC AAAGAGGAAC TTGCGCAAGA GTAGAGAAGA TCTGTCAGCT 
CAGCCCGTTC AGACCAAGTT CCCTGCTTAT GAAAGGGTGG TTCTTAGAGA GGCTGGTTTC 
CTGAGACCTA TTGTGATCTT TGGACCAATC GCAGATGTGG CACGAGACAA ACTGGCAAGA 
GAGGTGCCTG ACCTATTCGA GCTCGCAAAG ACACAGCTCC CAGATGGAAG GGAAAAGAGT 
GAACCTAGAG ATGCCGGCAC TGACCAGCGC AGCTCGGGGA TCATACGCCT TCACACTATC 
AAACAGATAA TCGACCGGGA TAAGCATGCA GTGCTGGACA TTACTCCTAA CGCAGTGGAT 
CGGCTGAACT ATGCTCAGTG GTATCCCATC GTAGTGTTCT TAAATCCTGA CAGCAAGCAG 
GGTGTGAAGA ACATGAGGAC CAGACTGTGT TCCGAGTCCA GGAAAAGTGC CAGAAAACTC 
CACGAGCGCG CACTCAAACT GAGGAAGAAC AACCATCATC TCTTCACAGC CACTATTAAC 
ATGAATAACA TGAATGATGG CTGGTATGGA GCACTGAAGG ACACCATTCA GCAGCAGCAG 
AACCAGCTGG TGTGGGTCTC AGAGGGCAAG GCGGACGGTT CACATGATGA TGACCTGGAC 
CTTCACGATG ACCGTCTGTC CTATTTATCG GCCCCAGGCA GTGAGTACAG TATGTACAGC 
ACGGACAGCC GCCACACCTC AGATTACGAG GACACGGACA CCGAGGGTGG CGCCTACACT 
GACCAGGAAC TGGACGAAAC CCTGAACGAT GAGGTCGGAC CACCCAGCGA ACCAGCCATC 
ACTCGCTCCT CAGAACCTGT CCGCGAAGAC CCGCCCGTCA TCCAGGAGCC CCCCGGATAT 
GGAGGATATC AGCCCGAACC CATCAACCGC ATCGACCCCG CGGGGTTCAA GGCCCCTGCA 
CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC TCGCAGCCTG AGCCACTGGC AGAGACAGCG 
CCCCCTGCTG TTCATGTAAA TGTAGGGGCC CCGAGTGCCA AGGATCTCAC TCAGGGGGAA 
ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA CCTGCCCCTG AGCTTATCCA ACCCCCGTCT 
CCTGACCCCA ACCAATCCGG ACAGCCCGGG TCAGAGTCCA AGATGTACAA GAAGGATATC 
TACCGACCCA ACGAACCGGT GCAGATAAGC CAAAACCCTA TGCAGCCCAA CTACTACCAG 
GAAGACAAGC CATACAGAGA TTATGACCAC CAGCCCTACC GCTATGATGG AGGCTACATG 
GAACCAAAGG CTCGTAACTT TGACCCACAT TTTGAGGACA GCGTGCCTCC ATATAATGAT 
CAATGGGGTC AGTATGACCA CACCTCTGGG TATGAACCTC AAACGCTCTA CGAGGATGGT 
CTGAACCGCT TGTATGGTCA CCCTCAGTCA CGAAACGACA CCGGCTACGA CACAGGTCGA 
CCTCGATATG GCAAACCAAC CTCTGGACCA ATCCGACACG ATGAACCACC ACCATCACGC 
CCACAATATG ACACTCAACA AACCTCTCGT TCCCCAGAAC CGCCCAAACA ACAATACTAC 
GACCCTTCCC AAAGGCCTTC CTACACTCAA GCTCCACAAA AAGGCTATAT GAACTCTGAA 
GCACCGGTAA CGCCACCCAA ACCTGAGTCG CTCTCACCGC CTTTGGAGCC TGCTCTTCCT 
CCTCCACCTC CTCCAGCCGA AGCAGAGGAT GATCCCGCCA TGAAGCCCCA GTCGGTGCTC 
ACCAGGGTGA AGATGTTCGA GAACAAGCGA TCGGTGTCTG TAGACAGAGC CAGAGAGTCT 
GCAGACTCTG GTATTCGGCC TGTGGACATG GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC 
AAAGCCAATT CTTTAAGTAA CCTGGATCAA GAGAAGACCT TTAGAACTCC AGAGCCCCAG 
CAGCCACAGC CTAAAGTAGA TGATATCATT CGTTCAAATA ACAATGACCC TGATGAGGAT 
GAAGAATATT ACAGGAAACA ACTGTCCTAC TTCGACCGCA GGAGCTTCGA CAAACCCTCC 
ACACAGCTGA CACCTGCCAT CAAACCAGCC CAACCCCAAA CTCAACCAGC ACACTATCCC 
AGAGCAGAGT CAGTGGAGAA AATCAACCCA GCTGCTCCAT CAAACCCAGC TGTCCCACCA 
CCAACCCTGC CTAAACCTGC AGCAACCCCA CCTCTCGACC CTCACGGATC TCCCAAAGTA 
AAGCCTCCTA GTCATGAAGA CACAGTCCAG AGCACTTACC TGCCTCACAA AAGCTTCCCA 
GAGAAATCCC CCGTCAACGG CACCGGCGAA CCTCCAAAGA CCAACAGCAC TGCCACGTCC 
AGCTACAACC GTTTTGTGCC CAAGCCGTAC ACCACCTCCG CAAGACCCTT CGAGCGCAAG 
TTTGACAGTC CCAAGTTCAA TCACAATCTC CTCCCTAATG ACTCCCAAAA CAAGACGGAT 
AATGTGAAAG CTCCCATCAC CGCTAAACCA CAGAGCTCAC CCCAGCCGGC AGACCACGAC 
AGCGGCCTGG ACACCTTCAC ACGCACCATG GACCACAAAC CCAAATACCA GCAGAACAAC 
ATCAACGCCG TACCCAAGGC AATACCTGTC AGCCCGAGTG CTTTGGAGGA TGATGACGAT 
GATGACAGCC ACACGGTGGT GGCGACAGCG CGGGGTATTT TCAACTCTAA TGGTGGGGTC 
CTGAGCTCCA TTGAGACAGG AGTAAGCATT ATTATCCCAC AGGGAGCCAT CCCTGATGGC 
GTGGAGCAAG AGATCTACTT TAAAGTGTGT CGGGATAACA GCATCCTGCC ACCTCTGGAC 
AAAGAGAAAG GAGAAACTCT GTTGAGTCCT CTGGTGATGT GTGGTCCTCA CGGTCTGAAA 
TTCCTGAAGC CAGTGGAGCT CCGCTTACCT CACTGTGCGT CTATGACCCC TGATGGTTGG 
TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGATC CGAAGAGTTG GCAGAACAAA 
TCTCTTCCCG GAGATCCCAA CTACCTGGTT GGAGCCAACT GTGTGTCTGT GCTTATTGAC 
CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168990.1
CDS	410..5578
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005168933.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168933.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGCAGGCC CAGTATGAAG 
CTGGTGAAGT TTAAGAAAGG GGAAAGTGTC GGATTGAGGC TGGCTGGAGG AAACGATGTG 
GGGATCTTTG TGGCTGGAGT TCTGGAGGAC AGTCCTGCTG CTAAGGAAGG CCTGGAGGAG 
GGAGACCAGA TACTCAGGGT TAACAATGTT GACTTTGCAA ACATTATTCG TGAGGAAGCA 
GTTCTGTTCC TGCTAGATCT GCCCAGAGGT GAGGAGGTCA CCATCCTTGC GCAGAAGAAG 
AAAGATGTGT ACCGGCGAAT AGTGGAGTCT GATGTGGGCG ACTCCTTCTA CATCAGGACA 
CACTTTGAGT ACGAGAAAGA GTCTCCTTAT GGGCTGAGCT TTAATAAAGG AGAGGTGTTC 
AGAGTGGTGG ACACTCTCTA CAATGGCAAA CTGGGCTCCT GGCTGGCTAT CCGCATCGGG 
AAGAACCACC AGGAGGTGGA AAGAGGCATC ATTCCCAACA AGAACAGGGC GGAACAGTTA 
TCAAGCGTAC AGTACACACT CCCCAAGACT GCAGGTGGTG ATCGTGCTGA TTTCTGGAGG 
TTCAGAGGCC TGCGCAGTTC AAAGAGGAAC TTGCGCAAGA GTAGAGAAGA TCTGTCAGCT 
CAGCCCGTTC AGACCAAGTT CCCTGCTTAT GAAAGGGTGG TTCTTAGAGA GGCTGGTTTC 
CTGAGACCTA TTGTGATCTT TGGACCAATC GCAGATGTGG CACGAGACAA ACTGGCAAGA 
GAGGTGCCTG ACCTATTCGA GCTCGCAAAG ACACAGCTCC CAGATGGAAG GGAAAAGAGT 
GAACCTAGAG ATGCCGGCAC TGACCAGCGC AGCTCGGGGA TCATACGCCT TCACACTATC 
AAACAGATAA TCGACCGGGA TAAGCATGCA GTGCTGGACA TTACTCCTAA CGCAGTGGAT 
CGGCTGAACT ATGCTCAGTG GTATCCCATC GTAGTGTTCT TAAATCCTGA CAGCAAGCAG 
GGTGTGAAGA ACATGAGGAC CAGACTGTGT TCCGAGTCCA GGAAAAGTGC CAGAAAACTC 
CACGAGCGCG CACTCAAACT GAGGAAGAAC AACCATCATC TCTTCACAGC CACTATTAAC 
ATGAATAACA TGAATGATGG CTGGTATGGA GCACTGAAGG ACACCATTCA GCAGCAGCAG 
AACCAGCTGG TGTGGGTCTC AGAGGGCAAG GCGGACGGTT CACATGATGA TGACCTGGAC 
CTTCACGATG ACCGTCTGTC CTATTTATCG GCCCCAGGCA GTGAGTACAG TATGTACAGC 
ACGGACAGCC GCCACACCTC AGATTACGAG GACACGGACA CCGAGGGTGG CGCCTACACT 
GACCAGGAAC TGGACGAAAC CCTGAACGAT GAGGTCGGAC CACCCAGCGA ACCAGCCATC 
ACTCGCTCCT CAGAACCTGT CCGCGAAGAC CCGCCCGTCA TCCAGGAGCC CCCCGGATAT 
GGAGGATATC AGCCCGAACC CATCAACCGC ATCGACCCCG CGGGGTTCAA GGCCCCTGCA 
CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC TCGCAGCCTG AGCCACTGGC AGAGACAGCG 
CCCCCTGCTG TTCATGTAAA TGTAGGGGCC CCGAGTGCCA AGGATCTCAC TCAGGGGGAA 
ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA CCTGCCCCTG AGCTTATCCA ACCCCCGTCT 
CCTGACCCCA ACCAATCCGG ACAGCCCGGG TCAGAGTCCA AGATGTACAA GAAGGATATC 
TACCGACCCA ACGAACCGGT GCAGATAAGC CAAAACCCTA TGCAGCCCAA CTACTACCAG 
GAAGACAAGC CATACAGAGA TTATGACCAC CAGCCCTACC GCTATGATGG AGGCTACATG 
GAACCAAAGG CTCGTAACTT TGACCCACAT TTTGAGGACA GCGTGCCTCC ATATAATGAT 
CAATGGGGTC AGTATGACCA CACCTCTGGG TATGAACCTC AAACGCTCTA CGAGGATGGT 
CTGAACCGCT TGTATGGTCA CCCTCAGTCA CGAAACGACA CCGGCTACGA CACAGGTCGA 
CCTCGATATG GCAAACCAAC CTCTGGACCA ATCCGACACG ATGAACCACC ACCATCACGC 
CCACAATATG ACACTCAACA AACCTCTCGT TCCCCAGAAC CGCCCAAACA ACAATACTAC 
GACCCTTCCC AAAGGCCTTC CTACACTCAA GCTCCACAAA AAGGCTATAT GAACTCTGAA 
GCACCGGTAA CGCCACCCAA ACCTGAGTCG CTCTCACCGC CTTTGGAGCC TGCTCTTCCT 
CCTCCACCTC CTCCAGCCGA AGCAGAGGAT GATCCCGCCA TGAAGCCCCA GTCGGTGCTC 
ACCAGGGTGA AGATGTTCGA GAACAAGCGA TCGGTGTCTG TAGACAGAGC CAGAGAGTCT 
GCAGACTCTG GTATTCGGCC TGTGGACATG GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC 
AAAGCCAATT CTTTAAGTAA CCTGGATCAA GAGAAGACCT TTAGAACTCC AGAGCCCCAG 
CAGCCACAGC CTAAAGTAGA TGATATCATT CGTTCAAATA ACAATGACCC TGATGAGGAT 
GAAGAATATT ACAGGAAACA ACTGTCCTAC TTCGACCGCA GGAGCTTCGA CAAACCCTCC 
ACACAGCTGA CACCTGCCAT CAAACCAGCC CAACCCCAAA CTCAACCAGC ACACTATCCC 
AGAGCAGAGT CAGTGGAGAA AATCAACCCA GCTGCTCCAT CAAACCCAGC TGTCCCACCA 
CCAACCCTGC CTAAACCTGC AGCAACCCCA CCTCTCGACC CTCACGGATC TCCCAAAGTA 
AAGCCTCCTA GTCATGAAGA CACAGTCCAG AGCACTTACC TGCCTCACAA AAGCTTCCCA 
GAGAAATCCC CCGTCAACGG CACCGGCGAA CCTCCAAAGA CCAACAGCAC TGCCACGTCC 
AGCTACAACC GTTTTGTGCC CAAGCCGTAC ACCACCTCCG CAAGACCCTT CGAGCGCAAG 
TTTGACAGTC CCAAGTTCAA TCACAATCTC CTCCCTAATG ACTCCCAAAA CAAGACGGAT 
AATGTGAAAG CTCCCATCAC CGCTAAACCA CAGAGCTCAC CCCAGCCGGC AGACCACGAC 
AGCGGCCTGG ACACCTTCAC ACGCACCATG GACCACAAAC CCAAATACCA GCAGAACAAC 
ATCAACGCCG TACCCAAGGC AATACCTGTC AGCCCGAGTG CTTTGGAGGA TGATGACGAT 
GATGACAGCC ACACGGTGGT GGCGACAGCG CGGGGTATTT TCAACTCTAA TGGTGGGGTC 
CTGAGCTCCA TTGAGACAGG AGTAAGCATT ATTATCCCAC AGGGAGCCAT CCCTGATGGC 
GTGGAGCAAG AGATCTACTT TAAAGTGTGT CGGGATAACA GCATCCTGCC ACCTCTGGAC 
AAAGAGAAAG GAGAAACTCT GTTGAGTCCT CTGGTGATGT GTGGTCCTCA CGGTCTGAAA 
TTCCTGAAGC CAGTGGAGCT CCGCTTACCT CACTGTGCGT CTATGACCCC TGATGGTTGG 
TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGATC CGAAGAGTTG GCAGAACAAA 
TCTCTTCCCG GAGATCCCAA CTACCTGGTT GGAGCCAACT GTGTGTCTGT GCTTATTGAC 
CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_005168933.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168990.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X4, mRNA.

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CloneID	ODa13132
Clone ID Related Accession (Same CDS sequence)	XM_005168933.3 , XM_005168933.4
Accession Version	XM_005168933.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168933.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGCAGGCC CAGTATGAAG 
CTGGTGAAGT TTAAGAAAGG GGAAAGTGTC GGATTGAGGC TGGCTGGAGG AAACGATGTG 
GGGATCTTTG TGGCTGGAGT TCTGGAGGAC AGTCCTGCTG CTAAGGAAGG CCTGGAGGAG 
GGAGACCAGA TACTCAGGGT TAACAATGTT GACTTTGCAA ACATTATTCG TGAGGAAGCA 
GTTCTGTTCC TGCTAGATCT GCCCAGAGGT GAGGAGGTCA CCATCCTTGC GCAGAAGAAG 
AAAGATGTGT ACCGGCGAAT AGTGGAGTCT GATGTGGGCG ACTCCTTCTA CATCAGGACA 
CACTTTGAGT ACGAGAAAGA GTCTCCTTAT GGGCTGAGCT TTAATAAAGG AGAGGTGTTC 
AGAGTGGTGG ACACTCTCTA CAATGGCAAA CTGGGCTCCT GGCTGGCTAT CCGCATCGGG 
AAGAACCACC AGGAGGTGGA AAGAGGCATC ATTCCCAACA AGAACAGGGC GGAACAGTTA 
TCAAGCGTAC AGTACACACT CCCCAAGACT GCAGGTGGTG ATCGTGCTGA TTTCTGGAGG 
TTCAGAGGCC TGCGCAGTTC AAAGAGGAAC TTGCGCAAGA GTAGAGAAGA TCTGTCAGCT 
CAGCCCGTTC AGACCAAGTT CCCTGCTTAT GAAAGGGTGG TTCTTAGAGA GGCTGGTTTC 
CTGAGACCTA TTGTGATCTT TGGACCAATC GCAGATGTGG CACGAGACAA ACTGGCAAGA 
GAGGTGCCTG ACCTATTCGA GCTCGCAAAG ACACAGCTCC CAGATGGAAG GGAAAAGAGT 
GAACCTAGAG ATGCCGGCAC TGACCAGCGC AGCTCGGGGA TCATACGCCT TCACACTATC 
AAACAGATAA TCGACCGGGA TAAGCATGCA GTGCTGGACA TTACTCCTAA CGCAGTGGAT 
CGGCTGAACT ATGCTCAGTG GTATCCCATC GTAGTGTTCT TAAATCCTGA CAGCAAGCAG 
GGTGTGAAGA ACATGAGGAC CAGACTGTGT TCCGAGTCCA GGAAAAGTGC CAGAAAACTC 
CACGAGCGCG CACTCAAACT GAGGAAGAAC AACCATCATC TCTTCACAGC CACTATTAAC 
ATGAATAACA TGAATGATGG CTGGTATGGA GCACTGAAGG ACACCATTCA GCAGCAGCAG 
AACCAGCTGG TGTGGGTCTC AGAGGGCAAG GCGGACGGTT CACATGATGA TGACCTGGAC 
CTTCACGATG ACCGTCTGTC CTATTTATCG GCCCCAGGCA GTGAGTACAG TATGTACAGC 
ACGGACAGCC GCCACACCTC AGATTACGAG GACACGGACA CCGAGGGTGG CGCCTACACT 
GACCAGGAAC TGGACGAAAC CCTGAACGAT GAGGTCGGAC CACCCAGCGA ACCAGCCATC 
ACTCGCTCCT CAGAACCTGT CCGCGAAGAC CCGCCCGTCA TCCAGGAGCC CCCCGGATAT 
GGAGGATATC AGCCCGAACC CATCAACCGC ATCGACCCCG CGGGGTTCAA GGCCCCTGCA 
CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC TCGCAGCCTG AGCCACTGGC AGAGACAGCG 
CCCCCTGCTG TTCATGTAAA TGTAGGGGCC CCGAGTGCCA AGGATCTCAC TCAGGGGGAA 
ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA CCTGCCCCTG AGCTTATCCA ACCCCCGTCT 
CCTGACCCCA ACCAATCCGG ACAGCCCGGG TCAGAGTCCA AGATGTACAA GAAGGATATC 
TACCGACCCA ACGAACCGGT GCAGATAAGC CAAAACCCTA TGCAGCCCAA CTACTACCAG 
GAAGACAAGC CATACAGAGA TTATGACCAC CAGCCCTACC GCTATGATGG AGGCTACATG 
GAACCAAAGG CTCGTAACTT TGACCCACAT TTTGAGGACA GCGTGCCTCC ATATAATGAT 
CAATGGGGTC AGTATGACCA CACCTCTGGG TATGAACCTC AAACGCTCTA CGAGGATGGT 
CTGAACCGCT TGTATGGTCA CCCTCAGTCA CGAAACGACA CCGGCTACGA CACAGGTCGA 
CCTCGATATG GCAAACCAAC CTCTGGACCA ATCCGACACG ATGAACCACC ACCATCACGC 
CCACAATATG ACACTCAACA AACCTCTCGT TCCCCAGAAC CGCCCAAACA ACAATACTAC 
GACCCTTCCC AAAGGCCTTC CTACACTCAA GCTCCACAAA AAGGCTATAT GAACTCTGAA 
GCACCGGTAA CGCCACCCAA ACCTGAGTCG CTCTCACCGC CTTTGGAGCC TGCTCTTCCT 
CCTCCACCTC CTCCAGCCGA AGCAGAGGAT GATCCCGCCA TGAAGCCCCA GTCGGTGCTC 
ACCAGGGTGA AGATGTTCGA GAACAAGCGA TCGGTGTCTG TAGACAGAGC CAGAGAGTCT 
GCAGACTCTG GTATTCGGCC TGTGGACATG GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC 
AAAGCCAATT CTTTAAGTAA CCTGGATCAA GAGAAGACCT TTAGAACTCC AGAGCCCCAG 
CAGCCACAGC CTAAAGTAGA TGATATCATT CGTTCAAATA ACAATGACCC TGATGAGGAT 
GAAGAATATT ACAGGAAACA ACTGTCCTAC TTCGACCGCA GGAGCTTCGA CAAACCCTCC 
ACACAGCTGA CACCTGCCAT CAAACCAGCC CAACCCCAAA CTCAACCAGC ACACTATCCC 
AGAGCAGAGT CAGTGGAGAA AATCAACCCA GCTGCTCCAT CAAACCCAGC TGTCCCACCA 
CCAACCCTGC CTAAACCTGC AGCAACCCCA CCTCTCGACC CTCACGGATC TCCCAAAGTA 
AAGCCTCCTA GTCATGAAGA CACAGTCCAG AGCACTTACC TGCCTCACAA AAGCTTCCCA 
GAGAAATCCC CCGTCAACGG CACCGGCGAA CCTCCAAAGA CCAACAGCAC TGCCACGTCC 
AGCTACAACC GTTTTGTGCC CAAGCCGTAC ACCACCTCCG CAAGACCCTT CGAGCGCAAG 
TTTGACAGTC CCAAGTTCAA TCACAATCTC CTCCCTAATG ACTCCCAAAA CAAGACGGAT 
AATGTGAAAG CTCCCATCAC CGCTAAACCA CAGAGCTCAC CCCAGCCGGC AGACCACGAC 
AGCGGCCTGG ACACCTTCAC ACGCACCATG GACCACAAAC CCAAATACCA GCAGAACAAC 
ATCAACGCCG TACCCAAGGC AATACCTGTC AGCCCGAGTG CTTTGGAGGA TGATGACGAT 
GATGACAGCC ACACGGTGGT GGCGACAGCG CGGGGTATTT TCAACTCTAA TGGTGGGGTC 
CTGAGCTCCA TTGAGACAGG AGTAAGCATT ATTATCCCAC AGGGAGCCAT CCCTGATGGC 
GTGGAGCAAG AGATCTACTT TAAAGTGTGT CGGGATAACA GCATCCTGCC ACCTCTGGAC 
AAAGAGAAAG GAGAAACTCT GTTGAGTCCT CTGGTGATGT GTGGTCCTCA CGGTCTGAAA 
TTCCTGAAGC CAGTGGAGCT CCGCTTACCT CACTGTGCGT CTATGACCCC TGATGGTTGG 
TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGATC CGAAGAGTTG GCAGAACAAA 
TCTCTTCCCG GAGATCCCAA CTACCTGGTT GGAGCCAACT GTGTGTCTGT GCTTATTGAC 
CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168990.1
CDS	384..5552
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005168933.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168933.4

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGCAGGCC CAGTATGAAG 
CTGGTGAAGT TTAAGAAAGG GGAAAGTGTC GGATTGAGGC TGGCTGGAGG AAACGATGTG 
GGGATCTTTG TGGCTGGAGT TCTGGAGGAC AGTCCTGCTG CTAAGGAAGG CCTGGAGGAG 
GGAGACCAGA TACTCAGGGT TAACAATGTT GACTTTGCAA ACATTATTCG TGAGGAAGCA 
GTTCTGTTCC TGCTAGATCT GCCCAGAGGT GAGGAGGTCA CCATCCTTGC GCAGAAGAAG 
AAAGATGTGT ACCGGCGAAT AGTGGAGTCT GATGTGGGCG ACTCCTTCTA CATCAGGACA 
CACTTTGAGT ACGAGAAAGA GTCTCCTTAT GGGCTGAGCT TTAATAAAGG AGAGGTGTTC 
AGAGTGGTGG ACACTCTCTA CAATGGCAAA CTGGGCTCCT GGCTGGCTAT CCGCATCGGG 
AAGAACCACC AGGAGGTGGA AAGAGGCATC ATTCCCAACA AGAACAGGGC GGAACAGTTA 
TCAAGCGTAC AGTACACACT CCCCAAGACT GCAGGTGGTG ATCGTGCTGA TTTCTGGAGG 
TTCAGAGGCC TGCGCAGTTC AAAGAGGAAC TTGCGCAAGA GTAGAGAAGA TCTGTCAGCT 
CAGCCCGTTC AGACCAAGTT CCCTGCTTAT GAAAGGGTGG TTCTTAGAGA GGCTGGTTTC 
CTGAGACCTA TTGTGATCTT TGGACCAATC GCAGATGTGG CACGAGACAA ACTGGCAAGA 
GAGGTGCCTG ACCTATTCGA GCTCGCAAAG ACACAGCTCC CAGATGGAAG GGAAAAGAGT 
GAACCTAGAG ATGCCGGCAC TGACCAGCGC AGCTCGGGGA TCATACGCCT TCACACTATC 
AAACAGATAA TCGACCGGGA TAAGCATGCA GTGCTGGACA TTACTCCTAA CGCAGTGGAT 
CGGCTGAACT ATGCTCAGTG GTATCCCATC GTAGTGTTCT TAAATCCTGA CAGCAAGCAG 
GGTGTGAAGA ACATGAGGAC CAGACTGTGT TCCGAGTCCA GGAAAAGTGC CAGAAAACTC 
CACGAGCGCG CACTCAAACT GAGGAAGAAC AACCATCATC TCTTCACAGC CACTATTAAC 
ATGAATAACA TGAATGATGG CTGGTATGGA GCACTGAAGG ACACCATTCA GCAGCAGCAG 
AACCAGCTGG TGTGGGTCTC AGAGGGCAAG GCGGACGGTT CACATGATGA TGACCTGGAC 
CTTCACGATG ACCGTCTGTC CTATTTATCG GCCCCAGGCA GTGAGTACAG TATGTACAGC 
ACGGACAGCC GCCACACCTC AGATTACGAG GACACGGACA CCGAGGGTGG CGCCTACACT 
GACCAGGAAC TGGACGAAAC CCTGAACGAT GAGGTCGGAC CACCCAGCGA ACCAGCCATC 
ACTCGCTCCT CAGAACCTGT CCGCGAAGAC CCGCCCGTCA TCCAGGAGCC CCCCGGATAT 
GGAGGATATC AGCCCGAACC CATCAACCGC ATCGACCCCG CGGGGTTCAA GGCCCCTGCA 
CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC TCGCAGCCTG AGCCACTGGC AGAGACAGCG 
CCCCCTGCTG TTCATGTAAA TGTAGGGGCC CCGAGTGCCA AGGATCTCAC TCAGGGGGAA 
ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA CCTGCCCCTG AGCTTATCCA ACCCCCGTCT 
CCTGACCCCA ACCAATCCGG ACAGCCCGGG TCAGAGTCCA AGATGTACAA GAAGGATATC 
TACCGACCCA ACGAACCGGT GCAGATAAGC CAAAACCCTA TGCAGCCCAA CTACTACCAG 
GAAGACAAGC CATACAGAGA TTATGACCAC CAGCCCTACC GCTATGATGG AGGCTACATG 
GAACCAAAGG CTCGTAACTT TGACCCACAT TTTGAGGACA GCGTGCCTCC ATATAATGAT 
CAATGGGGTC AGTATGACCA CACCTCTGGG TATGAACCTC AAACGCTCTA CGAGGATGGT 
CTGAACCGCT TGTATGGTCA CCCTCAGTCA CGAAACGACA CCGGCTACGA CACAGGTCGA 
CCTCGATATG GCAAACCAAC CTCTGGACCA ATCCGACACG ATGAACCACC ACCATCACGC 
CCACAATATG ACACTCAACA AACCTCTCGT TCCCCAGAAC CGCCCAAACA ACAATACTAC 
GACCCTTCCC AAAGGCCTTC CTACACTCAA GCTCCACAAA AAGGCTATAT GAACTCTGAA 
GCACCGGTAA CGCCACCCAA ACCTGAGTCG CTCTCACCGC CTTTGGAGCC TGCTCTTCCT 
CCTCCACCTC CTCCAGCCGA AGCAGAGGAT GATCCCGCCA TGAAGCCCCA GTCGGTGCTC 
ACCAGGGTGA AGATGTTCGA GAACAAGCGA TCGGTGTCTG TAGACAGAGC CAGAGAGTCT 
GCAGACTCTG GTATTCGGCC TGTGGACATG GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC 
AAAGCCAATT CTTTAAGTAA CCTGGATCAA GAGAAGACCT TTAGAACTCC AGAGCCCCAG 
CAGCCACAGC CTAAAGTAGA TGATATCATT CGTTCAAATA ACAATGACCC TGATGAGGAT 
GAAGAATATT ACAGGAAACA ACTGTCCTAC TTCGACCGCA GGAGCTTCGA CAAACCCTCC 
ACACAGCTGA CACCTGCCAT CAAACCAGCC CAACCCCAAA CTCAACCAGC ACACTATCCC 
AGAGCAGAGT CAGTGGAGAA AATCAACCCA GCTGCTCCAT CAAACCCAGC TGTCCCACCA 
CCAACCCTGC CTAAACCTGC AGCAACCCCA CCTCTCGACC CTCACGGATC TCCCAAAGTA 
AAGCCTCCTA GTCATGAAGA CACAGTCCAG AGCACTTACC TGCCTCACAA AAGCTTCCCA 
GAGAAATCCC CCGTCAACGG CACCGGCGAA CCTCCAAAGA CCAACAGCAC TGCCACGTCC 
AGCTACAACC GTTTTGTGCC CAAGCCGTAC ACCACCTCCG CAAGACCCTT CGAGCGCAAG 
TTTGACAGTC CCAAGTTCAA TCACAATCTC CTCCCTAATG ACTCCCAAAA CAAGACGGAT 
AATGTGAAAG CTCCCATCAC CGCTAAACCA CAGAGCTCAC CCCAGCCGGC AGACCACGAC 
AGCGGCCTGG ACACCTTCAC ACGCACCATG GACCACAAAC CCAAATACCA GCAGAACAAC 
ATCAACGCCG TACCCAAGGC AATACCTGTC AGCCCGAGTG CTTTGGAGGA TGATGACGAT 
GATGACAGCC ACACGGTGGT GGCGACAGCG CGGGGTATTT TCAACTCTAA TGGTGGGGTC 
CTGAGCTCCA TTGAGACAGG AGTAAGCATT ATTATCCCAC AGGGAGCCAT CCCTGATGGC 
GTGGAGCAAG AGATCTACTT TAAAGTGTGT CGGGATAACA GCATCCTGCC ACCTCTGGAC 
AAAGAGAAAG GAGAAACTCT GTTGAGTCCT CTGGTGATGT GTGGTCCTCA CGGTCTGAAA 
TTCCTGAAGC CAGTGGAGCT CCGCTTACCT CACTGTGCGT CTATGACCCC TGATGGTTGG 
TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGATC CGAAGAGTTG GCAGAACAAA 
TCTCTTCCCG GAGATCCCAA CTACCTGGTT GGAGCCAACT GTGTGTCTGT GCTTATTGAC 
CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_005168936.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168993.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X8, mRNA.

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CloneID	ODa13135
Clone ID Related Accession (Same CDS sequence)	XM_005168936.4 , XM_005168936.3
Accession Version	XM_005168936.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168936.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGCGT CTATGACCCC TGATGGTTGG TCTTTTGCTC TAAAATCCTC CGACTCCTCG 
TCGGGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168993.1
CDS	383..5521
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005168936.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168936.4

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGCGT CTATGACCCC TGATGGTTGG TCTTTTGCTC TAAAATCCTC CGACTCCTCG 
TCGGGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_005168936.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168993.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X7, mRNA.

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CloneID	ODa13135
Clone ID Related Accession (Same CDS sequence)	XM_005168936.4 , XM_005168936.3
Accession Version	XM_005168936.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGCGT CTATGACCCC TGATGGTTGG TCTTTTGCTC TAAAATCCTC CGACTCCTCG 
TCGGGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168993.1
CDS	409..5547
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005168936.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168936.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGCGT CTATGACCCC TGATGGTTGG TCTTTTGCTC TAAAATCCTC CGACTCCTCG 
TCGGGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303250.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301525.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X1, mRNA.

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CloneID	ODa40325
Clone ID Related Accession (Same CDS sequence)	XM_009303250.3 , XM_009303250.2
Accession Version	XM_009303250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTTGGTCTT TTGCTCTAAA ATCCTCCGAC 
TCCTCGTCGG GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301525.1
CDS	384..5588
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303250.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303250.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTTGGTCTT TTGCTCTAAA ATCCTCCGAC 
TCCTCGTCGG GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303250.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301525.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X1, mRNA.

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CloneID	ODa40325
Clone ID Related Accession (Same CDS sequence)	XM_009303250.3 , XM_009303250.2
Accession Version	XM_009303250.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTTGGTCTT TTGCTCTAAA ATCCTCCGAC 
TCCTCGTCGG GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301525.1
CDS	410..5614
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303250.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303250.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTTGGTCTT TTGCTCTAAA ATCCTCCGAC 
TCCTCGTCGG GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303251.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301526.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40326
Clone ID Related Accession (Same CDS sequence)	XM_009303251.2 , XM_009303251.3
Accession Version	XM_009303251.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG GCCCAGTATG AAGCTGGTGA AGTTTAAGAA AGGGGAAAGT 
GTCGGATTGA GGCTGGCTGG AGGAAACGAT GTGGGGATCT TTGTGGCTGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAGGA AGGCCTGGAG GAGGGAGACC AGATACTCAG GGTTAACAAT 
GTTGACTTTG CAAACATTAT TCGTGAGGAA GCAGTTCTGT TCCTGCTAGA TCTGCCCAGA 
GGTGAGGAGG TCACCATCCT TGCGCAGAAG AAGAAAGATG TGTACCGGCG AATAGTGGAG 
TCTGATGTGG GCGACTCCTT CTACATCAGG ACACACTTTG AGTACGAGAA AGAGTCTCCT 
TATGGGCTGA GCTTTAATAA AGGAGAGGTG TTCAGAGTGG TGGACACTCT CTACAATGGC 
AAACTGGGCT CCTGGCTGGC TATCCGCATC GGGAAGAACC ACCAGGAGGT GGAAAGAGGC 
ATCATTCCCA ACAAGAACAG GGCGGAACAG TTATCAAGCG TACAGTACAC ACTCCCCAAG 
ACTGCAGGTG GTGATCGTGC TGATTTCTGG AGGTTCAGAG GCCTGCGCAG TTCAAAGAGG 
AACTTGCGCA AGAGTAGAGA AGATCTGTCA GCTCAGCCCG TTCAGACCAA GTTCCCTGCT 
TATGAAAGGG TGGTTCTTAG AGAGGCTGGT TTCCTGAGAC CTATTGTGAT CTTTGGACCA 
ATCGCAGATG TGGCACGAGA CAAACTGGCA AGAGAGGTGC CTGACCTATT CGAGCTCGCA 
AAGACACAGC TCCCAGATGG AAGGGAAAAG AGTGAACCTA GAGATGCCGG CACTGACCAG 
CGCAGCTCGG GGATCATACG CCTTCACACT ATCAAACAGA TAATCGACCG GGATAAGCAT 
GCAGTGCTGG ACATTACTCC TAACGCAGTG GATCGGCTGA ACTATGCTCA GTGGTATCCC 
ATCGTAGTGT TCTTAAATCC TGACAGCAAG CAGGGTGTGA AGAACATGAG GACCAGACTG 
TGTTCCGAGT CCAGGAAAAG TGCCAGAAAA CTCCACGAGC GCGCACTCAA ACTGAGGAAG 
AACAACCATC ATCTCTTCAC AGCCACTATT AACATGAATA ACATGAATGA TGGCTGGTAT 
GGAGCACTGA AGGACACCAT TCAGCAGCAG CAGAACCAGC TGGTGTGGGT CTCAGAGGGC 
AAGGCGGACG GTTCACATGA TGATGACCTG GACCTTCACG ATGACCGTCT GTCCTATTTA 
TCGGCCCCAG GCAGTGAGTA CAGTATGTAC AGCACGGACA GCCGCCACAC CTCAGATTAC 
GAGGACACGG ACACCGAGGG TGGCGCCTAC ACTGACCAGG AACTGGACGA AACCCTGAAC 
GATGAGGTCG GACCACCCAG CGAACCAGCC ATCACTCGCT CCTCAGAACC TGTCCGCGAA 
GACCCGCCCG TCATCCAGGA GCCCCCCGGA TATGGAGGAT ATCAGCCCGA ACCCATCAAC 
CGCATCGACC CCGCGGGGTT CAAGGCCCCT GCACCTCAGC AGAAAGCAGA GGCTGCTCTC 
CCCTCGCAGC CTGAGCCACT GGCAGAGACA GCGCCCCCTG CTGTTCATGT AAATGTAGGG 
GCCCCGAGTG CCAAGGATCT CACTCAGGGG GAAATCAGCC CCAAGGCTGG CTCTCTCTTC 
CCACCTGCCC CTGAGCTTAT CCAACCCCCG TCTCCTGACC CCAACCAATC CGGACAGCCC 
GGGTCAGAGT CCAAGATGTA CAAGAAGGAT ATCTACCGAC CCAACGAACC GGTGCAGATA 
AGCCAAAACC CTATGCAGCC CAACTACTAC CAGGAAGACA AGCCATACAG AGATTATGAC 
CACCAGCCCT ACCGCTATGA TGGAGGCTAC ATGGAACCAA AGGCTCGTAA CTTTGACCCA 
CATTTTGAGG ACAGCGTGCC TCCATATAAT GATCAATGGG GTCAGTATGA CCACACCTCT 
GGGTATGAAC CTCAAACGCT CTACGAGGAT GGTCTGAACC GCTTGTATGG TCACCCTCAG 
TCACGAAACG ACACCGGCTA CGACACAGGT CGACCTCGAT ATGGCAAACC AACCTCTGGA 
CCAATCCGAC ACGATGAACC ACCACCATCA CGCCCACAAT ATGACACTCA ACAAACCTCT 
CGTTCCCCAG AACCGCCCAA ACAACAATAC TACGACCCTT CCCAAAGGCC TTCCTACACT 
CAAGCTCCAC AAAAAGGCTA TATGAACTCT GAAGCACCGG TAACGCCACC CAAACCTGAG 
TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG 
GATGATCCCG CCATGAAGCC CCAGTCGGTG CTCACCAGGG TGAAGATGTT CGAGAACAAG 
CGATCGGTGT CTGTAGACAG AGCCAGAGAG TCTGCAGACT CTGGTATTCG GCCTGTGGAC 
ATGGTGCCTA AACCTGGCGC TGCCTCCGTG CCCAAAGCCA ATTCTTTAAG TAACCTGGAT 
CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC CAGCAGCCAC AGCCTAAAGT AGATGATATC 
ATTCGTTCAA ATAACAATGA CCCTGATGAG GATGAAGAAT ATTACAGGAA ACAACTGTCC 
TACTTCGACC GCAGGAGCTT CGACAAACCC TCCACACAGC TGACACCTGC CATCAAACCA 
GCCCAACCCC AAACTCAACC AGCACACTAT CCCAGAGCAG AGTCAGTGGA GAAAATCAAC 
CCAGCTGCTC CATCAAACCC AGCTGTCCCA CCACCAACCC TGCCTAAACC TGCAGCAACC 
CCACCTCTCG ACCCTCACGG ATCTCCCAAA GTAAAGCCTC CTAGTCATGA AGACACAGTC 
CAGAGCACTT ACCTGCCTCA CAAAAGCTTC CCAGAGAAAT CCCCCGTCAA CGGCACCGGC 
GAACCTCCAA AGACCAACAG CACTGCCACG TCCAGCTACA ACCGTTTTGT GCCCAAGCCG 
TACACCACCT CCGCAAGACC CTTCGAGCGC AAGTTTGACA GTCCCAAGTT CAATCACAAT 
CTCCTCCCTA ATGACTCCCA AAACAAGACG GATAATGTGA AAGCTCCCAT CACCGCTAAA 
CCACAGAGCT CACCCCAGCC GGCAGACCAC GACAGCGGCC TGGACACCTT CACACGCACC 
ATGGACCACA AACCCAAATA CCAGCAGAAC AACATCAACG CCGTACCCAA GGCAATACCT 
GTCAGCCCGA GTGCTTTGGA GGATGATGAC GATGATGACA GCCACACGGT GGTGGCGACA 
GCGCGGGGTA TTTTCAACTC TAATGGTGGG GTCCTGAGCT CCATTGAGAC AGGAGTAAGC 
ATTATTATCC CACAGGGAGC CATCCCTGAT GGCGTGGAGC AAGAGATCTA CTTTAAAGTG 
TGTCGGGATA ACAGCATCCT GCCACCTCTG GACAAAGAGA AAGGAGAAAC TCTGTTGAGT 
CCTCTGGTGA TGTGTGGTCC TCACGGTCTG AAATTCCTGA AGCCAGTGGA GCTCCGCTTA 
CCTCACTGTG CGTCTATGAC CCCTGATGGT TGGTCTTTTG CTCTAAAATC CTCCGACTCC 
TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC AAATCTCTTC CCGGAGATCC CAACTACCTG 
GTTGGAGCCA ACTGTGTGTC TGTGCTTATT GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301526.1
CDS	410..5611
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303251.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303251.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG GCCCAGTATG AAGCTGGTGA AGTTTAAGAA AGGGGAAAGT 
GTCGGATTGA GGCTGGCTGG AGGAAACGAT GTGGGGATCT TTGTGGCTGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAGGA AGGCCTGGAG GAGGGAGACC AGATACTCAG GGTTAACAAT 
GTTGACTTTG CAAACATTAT TCGTGAGGAA GCAGTTCTGT TCCTGCTAGA TCTGCCCAGA 
GGTGAGGAGG TCACCATCCT TGCGCAGAAG AAGAAAGATG TGTACCGGCG AATAGTGGAG 
TCTGATGTGG GCGACTCCTT CTACATCAGG ACACACTTTG AGTACGAGAA AGAGTCTCCT 
TATGGGCTGA GCTTTAATAA AGGAGAGGTG TTCAGAGTGG TGGACACTCT CTACAATGGC 
AAACTGGGCT CCTGGCTGGC TATCCGCATC GGGAAGAACC ACCAGGAGGT GGAAAGAGGC 
ATCATTCCCA ACAAGAACAG GGCGGAACAG TTATCAAGCG TACAGTACAC ACTCCCCAAG 
ACTGCAGGTG GTGATCGTGC TGATTTCTGG AGGTTCAGAG GCCTGCGCAG TTCAAAGAGG 
AACTTGCGCA AGAGTAGAGA AGATCTGTCA GCTCAGCCCG TTCAGACCAA GTTCCCTGCT 
TATGAAAGGG TGGTTCTTAG AGAGGCTGGT TTCCTGAGAC CTATTGTGAT CTTTGGACCA 
ATCGCAGATG TGGCACGAGA CAAACTGGCA AGAGAGGTGC CTGACCTATT CGAGCTCGCA 
AAGACACAGC TCCCAGATGG AAGGGAAAAG AGTGAACCTA GAGATGCCGG CACTGACCAG 
CGCAGCTCGG GGATCATACG CCTTCACACT ATCAAACAGA TAATCGACCG GGATAAGCAT 
GCAGTGCTGG ACATTACTCC TAACGCAGTG GATCGGCTGA ACTATGCTCA GTGGTATCCC 
ATCGTAGTGT TCTTAAATCC TGACAGCAAG CAGGGTGTGA AGAACATGAG GACCAGACTG 
TGTTCCGAGT CCAGGAAAAG TGCCAGAAAA CTCCACGAGC GCGCACTCAA ACTGAGGAAG 
AACAACCATC ATCTCTTCAC AGCCACTATT AACATGAATA ACATGAATGA TGGCTGGTAT 
GGAGCACTGA AGGACACCAT TCAGCAGCAG CAGAACCAGC TGGTGTGGGT CTCAGAGGGC 
AAGGCGGACG GTTCACATGA TGATGACCTG GACCTTCACG ATGACCGTCT GTCCTATTTA 
TCGGCCCCAG GCAGTGAGTA CAGTATGTAC AGCACGGACA GCCGCCACAC CTCAGATTAC 
GAGGACACGG ACACCGAGGG TGGCGCCTAC ACTGACCAGG AACTGGACGA AACCCTGAAC 
GATGAGGTCG GACCACCCAG CGAACCAGCC ATCACTCGCT CCTCAGAACC TGTCCGCGAA 
GACCCGCCCG TCATCCAGGA GCCCCCCGGA TATGGAGGAT ATCAGCCCGA ACCCATCAAC 
CGCATCGACC CCGCGGGGTT CAAGGCCCCT GCACCTCAGC AGAAAGCAGA GGCTGCTCTC 
CCCTCGCAGC CTGAGCCACT GGCAGAGACA GCGCCCCCTG CTGTTCATGT AAATGTAGGG 
GCCCCGAGTG CCAAGGATCT CACTCAGGGG GAAATCAGCC CCAAGGCTGG CTCTCTCTTC 
CCACCTGCCC CTGAGCTTAT CCAACCCCCG TCTCCTGACC CCAACCAATC CGGACAGCCC 
GGGTCAGAGT CCAAGATGTA CAAGAAGGAT ATCTACCGAC CCAACGAACC GGTGCAGATA 
AGCCAAAACC CTATGCAGCC CAACTACTAC CAGGAAGACA AGCCATACAG AGATTATGAC 
CACCAGCCCT ACCGCTATGA TGGAGGCTAC ATGGAACCAA AGGCTCGTAA CTTTGACCCA 
CATTTTGAGG ACAGCGTGCC TCCATATAAT GATCAATGGG GTCAGTATGA CCACACCTCT 
GGGTATGAAC CTCAAACGCT CTACGAGGAT GGTCTGAACC GCTTGTATGG TCACCCTCAG 
TCACGAAACG ACACCGGCTA CGACACAGGT CGACCTCGAT ATGGCAAACC AACCTCTGGA 
CCAATCCGAC ACGATGAACC ACCACCATCA CGCCCACAAT ATGACACTCA ACAAACCTCT 
CGTTCCCCAG AACCGCCCAA ACAACAATAC TACGACCCTT CCCAAAGGCC TTCCTACACT 
CAAGCTCCAC AAAAAGGCTA TATGAACTCT GAAGCACCGG TAACGCCACC CAAACCTGAG 
TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG 
GATGATCCCG CCATGAAGCC CCAGTCGGTG CTCACCAGGG TGAAGATGTT CGAGAACAAG 
CGATCGGTGT CTGTAGACAG AGCCAGAGAG TCTGCAGACT CTGGTATTCG GCCTGTGGAC 
ATGGTGCCTA AACCTGGCGC TGCCTCCGTG CCCAAAGCCA ATTCTTTAAG TAACCTGGAT 
CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC CAGCAGCCAC AGCCTAAAGT AGATGATATC 
ATTCGTTCAA ATAACAATGA CCCTGATGAG GATGAAGAAT ATTACAGGAA ACAACTGTCC 
TACTTCGACC GCAGGAGCTT CGACAAACCC TCCACACAGC TGACACCTGC CATCAAACCA 
GCCCAACCCC AAACTCAACC AGCACACTAT CCCAGAGCAG AGTCAGTGGA GAAAATCAAC 
CCAGCTGCTC CATCAAACCC AGCTGTCCCA CCACCAACCC TGCCTAAACC TGCAGCAACC 
CCACCTCTCG ACCCTCACGG ATCTCCCAAA GTAAAGCCTC CTAGTCATGA AGACACAGTC 
CAGAGCACTT ACCTGCCTCA CAAAAGCTTC CCAGAGAAAT CCCCCGTCAA CGGCACCGGC 
GAACCTCCAA AGACCAACAG CACTGCCACG TCCAGCTACA ACCGTTTTGT GCCCAAGCCG 
TACACCACCT CCGCAAGACC CTTCGAGCGC AAGTTTGACA GTCCCAAGTT CAATCACAAT 
CTCCTCCCTA ATGACTCCCA AAACAAGACG GATAATGTGA AAGCTCCCAT CACCGCTAAA 
CCACAGAGCT CACCCCAGCC GGCAGACCAC GACAGCGGCC TGGACACCTT CACACGCACC 
ATGGACCACA AACCCAAATA CCAGCAGAAC AACATCAACG CCGTACCCAA GGCAATACCT 
GTCAGCCCGA GTGCTTTGGA GGATGATGAC GATGATGACA GCCACACGGT GGTGGCGACA 
GCGCGGGGTA TTTTCAACTC TAATGGTGGG GTCCTGAGCT CCATTGAGAC AGGAGTAAGC 
ATTATTATCC CACAGGGAGC CATCCCTGAT GGCGTGGAGC AAGAGATCTA CTTTAAAGTG 
TGTCGGGATA ACAGCATCCT GCCACCTCTG GACAAAGAGA AAGGAGAAAC TCTGTTGAGT 
CCTCTGGTGA TGTGTGGTCC TCACGGTCTG AAATTCCTGA AGCCAGTGGA GCTCCGCTTA 
CCTCACTGTG CGTCTATGAC CCCTGATGGT TGGTCTTTTG CTCTAAAATC CTCCGACTCC 
TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC AAATCTCTTC CCGGAGATCC CAACTACCTG 
GTTGGAGCCA ACTGTGTGTC TGTGCTTATT GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303251.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301526.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40326
Clone ID Related Accession (Same CDS sequence)	XM_009303251.2 , XM_009303251.3
Accession Version	XM_009303251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303251.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG GCCCAGTATG AAGCTGGTGA AGTTTAAGAA AGGGGAAAGT 
GTCGGATTGA GGCTGGCTGG AGGAAACGAT GTGGGGATCT TTGTGGCTGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAGGA AGGCCTGGAG GAGGGAGACC AGATACTCAG GGTTAACAAT 
GTTGACTTTG CAAACATTAT TCGTGAGGAA GCAGTTCTGT TCCTGCTAGA TCTGCCCAGA 
GGTGAGGAGG TCACCATCCT TGCGCAGAAG AAGAAAGATG TGTACCGGCG AATAGTGGAG 
TCTGATGTGG GCGACTCCTT CTACATCAGG ACACACTTTG AGTACGAGAA AGAGTCTCCT 
TATGGGCTGA GCTTTAATAA AGGAGAGGTG TTCAGAGTGG TGGACACTCT CTACAATGGC 
AAACTGGGCT CCTGGCTGGC TATCCGCATC GGGAAGAACC ACCAGGAGGT GGAAAGAGGC 
ATCATTCCCA ACAAGAACAG GGCGGAACAG TTATCAAGCG TACAGTACAC ACTCCCCAAG 
ACTGCAGGTG GTGATCGTGC TGATTTCTGG AGGTTCAGAG GCCTGCGCAG TTCAAAGAGG 
AACTTGCGCA AGAGTAGAGA AGATCTGTCA GCTCAGCCCG TTCAGACCAA GTTCCCTGCT 
TATGAAAGGG TGGTTCTTAG AGAGGCTGGT TTCCTGAGAC CTATTGTGAT CTTTGGACCA 
ATCGCAGATG TGGCACGAGA CAAACTGGCA AGAGAGGTGC CTGACCTATT CGAGCTCGCA 
AAGACACAGC TCCCAGATGG AAGGGAAAAG AGTGAACCTA GAGATGCCGG CACTGACCAG 
CGCAGCTCGG GGATCATACG CCTTCACACT ATCAAACAGA TAATCGACCG GGATAAGCAT 
GCAGTGCTGG ACATTACTCC TAACGCAGTG GATCGGCTGA ACTATGCTCA GTGGTATCCC 
ATCGTAGTGT TCTTAAATCC TGACAGCAAG CAGGGTGTGA AGAACATGAG GACCAGACTG 
TGTTCCGAGT CCAGGAAAAG TGCCAGAAAA CTCCACGAGC GCGCACTCAA ACTGAGGAAG 
AACAACCATC ATCTCTTCAC AGCCACTATT AACATGAATA ACATGAATGA TGGCTGGTAT 
GGAGCACTGA AGGACACCAT TCAGCAGCAG CAGAACCAGC TGGTGTGGGT CTCAGAGGGC 
AAGGCGGACG GTTCACATGA TGATGACCTG GACCTTCACG ATGACCGTCT GTCCTATTTA 
TCGGCCCCAG GCAGTGAGTA CAGTATGTAC AGCACGGACA GCCGCCACAC CTCAGATTAC 
GAGGACACGG ACACCGAGGG TGGCGCCTAC ACTGACCAGG AACTGGACGA AACCCTGAAC 
GATGAGGTCG GACCACCCAG CGAACCAGCC ATCACTCGCT CCTCAGAACC TGTCCGCGAA 
GACCCGCCCG TCATCCAGGA GCCCCCCGGA TATGGAGGAT ATCAGCCCGA ACCCATCAAC 
CGCATCGACC CCGCGGGGTT CAAGGCCCCT GCACCTCAGC AGAAAGCAGA GGCTGCTCTC 
CCCTCGCAGC CTGAGCCACT GGCAGAGACA GCGCCCCCTG CTGTTCATGT AAATGTAGGG 
GCCCCGAGTG CCAAGGATCT CACTCAGGGG GAAATCAGCC CCAAGGCTGG CTCTCTCTTC 
CCACCTGCCC CTGAGCTTAT CCAACCCCCG TCTCCTGACC CCAACCAATC CGGACAGCCC 
GGGTCAGAGT CCAAGATGTA CAAGAAGGAT ATCTACCGAC CCAACGAACC GGTGCAGATA 
AGCCAAAACC CTATGCAGCC CAACTACTAC CAGGAAGACA AGCCATACAG AGATTATGAC 
CACCAGCCCT ACCGCTATGA TGGAGGCTAC ATGGAACCAA AGGCTCGTAA CTTTGACCCA 
CATTTTGAGG ACAGCGTGCC TCCATATAAT GATCAATGGG GTCAGTATGA CCACACCTCT 
GGGTATGAAC CTCAAACGCT CTACGAGGAT GGTCTGAACC GCTTGTATGG TCACCCTCAG 
TCACGAAACG ACACCGGCTA CGACACAGGT CGACCTCGAT ATGGCAAACC AACCTCTGGA 
CCAATCCGAC ACGATGAACC ACCACCATCA CGCCCACAAT ATGACACTCA ACAAACCTCT 
CGTTCCCCAG AACCGCCCAA ACAACAATAC TACGACCCTT CCCAAAGGCC TTCCTACACT 
CAAGCTCCAC AAAAAGGCTA TATGAACTCT GAAGCACCGG TAACGCCACC CAAACCTGAG 
TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG 
GATGATCCCG CCATGAAGCC CCAGTCGGTG CTCACCAGGG TGAAGATGTT CGAGAACAAG 
CGATCGGTGT CTGTAGACAG AGCCAGAGAG TCTGCAGACT CTGGTATTCG GCCTGTGGAC 
ATGGTGCCTA AACCTGGCGC TGCCTCCGTG CCCAAAGCCA ATTCTTTAAG TAACCTGGAT 
CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC CAGCAGCCAC AGCCTAAAGT AGATGATATC 
ATTCGTTCAA ATAACAATGA CCCTGATGAG GATGAAGAAT ATTACAGGAA ACAACTGTCC 
TACTTCGACC GCAGGAGCTT CGACAAACCC TCCACACAGC TGACACCTGC CATCAAACCA 
GCCCAACCCC AAACTCAACC AGCACACTAT CCCAGAGCAG AGTCAGTGGA GAAAATCAAC 
CCAGCTGCTC CATCAAACCC AGCTGTCCCA CCACCAACCC TGCCTAAACC TGCAGCAACC 
CCACCTCTCG ACCCTCACGG ATCTCCCAAA GTAAAGCCTC CTAGTCATGA AGACACAGTC 
CAGAGCACTT ACCTGCCTCA CAAAAGCTTC CCAGAGAAAT CCCCCGTCAA CGGCACCGGC 
GAACCTCCAA AGACCAACAG CACTGCCACG TCCAGCTACA ACCGTTTTGT GCCCAAGCCG 
TACACCACCT CCGCAAGACC CTTCGAGCGC AAGTTTGACA GTCCCAAGTT CAATCACAAT 
CTCCTCCCTA ATGACTCCCA AAACAAGACG GATAATGTGA AAGCTCCCAT CACCGCTAAA 
CCACAGAGCT CACCCCAGCC GGCAGACCAC GACAGCGGCC TGGACACCTT CACACGCACC 
ATGGACCACA AACCCAAATA CCAGCAGAAC AACATCAACG CCGTACCCAA GGCAATACCT 
GTCAGCCCGA GTGCTTTGGA GGATGATGAC GATGATGACA GCCACACGGT GGTGGCGACA 
GCGCGGGGTA TTTTCAACTC TAATGGTGGG GTCCTGAGCT CCATTGAGAC AGGAGTAAGC 
ATTATTATCC CACAGGGAGC CATCCCTGAT GGCGTGGAGC AAGAGATCTA CTTTAAAGTG 
TGTCGGGATA ACAGCATCCT GCCACCTCTG GACAAAGAGA AAGGAGAAAC TCTGTTGAGT 
CCTCTGGTGA TGTGTGGTCC TCACGGTCTG AAATTCCTGA AGCCAGTGGA GCTCCGCTTA 
CCTCACTGTG CGTCTATGAC CCCTGATGGT TGGTCTTTTG CTCTAAAATC CTCCGACTCC 
TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC AAATCTCTTC CCGGAGATCC CAACTACCTG 
GTTGGAGCCA ACTGTGTGTC TGTGCTTATT GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301526.1
CDS	384..5585
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303251.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303251.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG GCCCAGTATG AAGCTGGTGA AGTTTAAGAA AGGGGAAAGT 
GTCGGATTGA GGCTGGCTGG AGGAAACGAT GTGGGGATCT TTGTGGCTGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAGGA AGGCCTGGAG GAGGGAGACC AGATACTCAG GGTTAACAAT 
GTTGACTTTG CAAACATTAT TCGTGAGGAA GCAGTTCTGT TCCTGCTAGA TCTGCCCAGA 
GGTGAGGAGG TCACCATCCT TGCGCAGAAG AAGAAAGATG TGTACCGGCG AATAGTGGAG 
TCTGATGTGG GCGACTCCTT CTACATCAGG ACACACTTTG AGTACGAGAA AGAGTCTCCT 
TATGGGCTGA GCTTTAATAA AGGAGAGGTG TTCAGAGTGG TGGACACTCT CTACAATGGC 
AAACTGGGCT CCTGGCTGGC TATCCGCATC GGGAAGAACC ACCAGGAGGT GGAAAGAGGC 
ATCATTCCCA ACAAGAACAG GGCGGAACAG TTATCAAGCG TACAGTACAC ACTCCCCAAG 
ACTGCAGGTG GTGATCGTGC TGATTTCTGG AGGTTCAGAG GCCTGCGCAG TTCAAAGAGG 
AACTTGCGCA AGAGTAGAGA AGATCTGTCA GCTCAGCCCG TTCAGACCAA GTTCCCTGCT 
TATGAAAGGG TGGTTCTTAG AGAGGCTGGT TTCCTGAGAC CTATTGTGAT CTTTGGACCA 
ATCGCAGATG TGGCACGAGA CAAACTGGCA AGAGAGGTGC CTGACCTATT CGAGCTCGCA 
AAGACACAGC TCCCAGATGG AAGGGAAAAG AGTGAACCTA GAGATGCCGG CACTGACCAG 
CGCAGCTCGG GGATCATACG CCTTCACACT ATCAAACAGA TAATCGACCG GGATAAGCAT 
GCAGTGCTGG ACATTACTCC TAACGCAGTG GATCGGCTGA ACTATGCTCA GTGGTATCCC 
ATCGTAGTGT TCTTAAATCC TGACAGCAAG CAGGGTGTGA AGAACATGAG GACCAGACTG 
TGTTCCGAGT CCAGGAAAAG TGCCAGAAAA CTCCACGAGC GCGCACTCAA ACTGAGGAAG 
AACAACCATC ATCTCTTCAC AGCCACTATT AACATGAATA ACATGAATGA TGGCTGGTAT 
GGAGCACTGA AGGACACCAT TCAGCAGCAG CAGAACCAGC TGGTGTGGGT CTCAGAGGGC 
AAGGCGGACG GTTCACATGA TGATGACCTG GACCTTCACG ATGACCGTCT GTCCTATTTA 
TCGGCCCCAG GCAGTGAGTA CAGTATGTAC AGCACGGACA GCCGCCACAC CTCAGATTAC 
GAGGACACGG ACACCGAGGG TGGCGCCTAC ACTGACCAGG AACTGGACGA AACCCTGAAC 
GATGAGGTCG GACCACCCAG CGAACCAGCC ATCACTCGCT CCTCAGAACC TGTCCGCGAA 
GACCCGCCCG TCATCCAGGA GCCCCCCGGA TATGGAGGAT ATCAGCCCGA ACCCATCAAC 
CGCATCGACC CCGCGGGGTT CAAGGCCCCT GCACCTCAGC AGAAAGCAGA GGCTGCTCTC 
CCCTCGCAGC CTGAGCCACT GGCAGAGACA GCGCCCCCTG CTGTTCATGT AAATGTAGGG 
GCCCCGAGTG CCAAGGATCT CACTCAGGGG GAAATCAGCC CCAAGGCTGG CTCTCTCTTC 
CCACCTGCCC CTGAGCTTAT CCAACCCCCG TCTCCTGACC CCAACCAATC CGGACAGCCC 
GGGTCAGAGT CCAAGATGTA CAAGAAGGAT ATCTACCGAC CCAACGAACC GGTGCAGATA 
AGCCAAAACC CTATGCAGCC CAACTACTAC CAGGAAGACA AGCCATACAG AGATTATGAC 
CACCAGCCCT ACCGCTATGA TGGAGGCTAC ATGGAACCAA AGGCTCGTAA CTTTGACCCA 
CATTTTGAGG ACAGCGTGCC TCCATATAAT GATCAATGGG GTCAGTATGA CCACACCTCT 
GGGTATGAAC CTCAAACGCT CTACGAGGAT GGTCTGAACC GCTTGTATGG TCACCCTCAG 
TCACGAAACG ACACCGGCTA CGACACAGGT CGACCTCGAT ATGGCAAACC AACCTCTGGA 
CCAATCCGAC ACGATGAACC ACCACCATCA CGCCCACAAT ATGACACTCA ACAAACCTCT 
CGTTCCCCAG AACCGCCCAA ACAACAATAC TACGACCCTT CCCAAAGGCC TTCCTACACT 
CAAGCTCCAC AAAAAGGCTA TATGAACTCT GAAGCACCGG TAACGCCACC CAAACCTGAG 
TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG 
GATGATCCCG CCATGAAGCC CCAGTCGGTG CTCACCAGGG TGAAGATGTT CGAGAACAAG 
CGATCGGTGT CTGTAGACAG AGCCAGAGAG TCTGCAGACT CTGGTATTCG GCCTGTGGAC 
ATGGTGCCTA AACCTGGCGC TGCCTCCGTG CCCAAAGCCA ATTCTTTAAG TAACCTGGAT 
CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC CAGCAGCCAC AGCCTAAAGT AGATGATATC 
ATTCGTTCAA ATAACAATGA CCCTGATGAG GATGAAGAAT ATTACAGGAA ACAACTGTCC 
TACTTCGACC GCAGGAGCTT CGACAAACCC TCCACACAGC TGACACCTGC CATCAAACCA 
GCCCAACCCC AAACTCAACC AGCACACTAT CCCAGAGCAG AGTCAGTGGA GAAAATCAAC 
CCAGCTGCTC CATCAAACCC AGCTGTCCCA CCACCAACCC TGCCTAAACC TGCAGCAACC 
CCACCTCTCG ACCCTCACGG ATCTCCCAAA GTAAAGCCTC CTAGTCATGA AGACACAGTC 
CAGAGCACTT ACCTGCCTCA CAAAAGCTTC CCAGAGAAAT CCCCCGTCAA CGGCACCGGC 
GAACCTCCAA AGACCAACAG CACTGCCACG TCCAGCTACA ACCGTTTTGT GCCCAAGCCG 
TACACCACCT CCGCAAGACC CTTCGAGCGC AAGTTTGACA GTCCCAAGTT CAATCACAAT 
CTCCTCCCTA ATGACTCCCA AAACAAGACG GATAATGTGA AAGCTCCCAT CACCGCTAAA 
CCACAGAGCT CACCCCAGCC GGCAGACCAC GACAGCGGCC TGGACACCTT CACACGCACC 
ATGGACCACA AACCCAAATA CCAGCAGAAC AACATCAACG CCGTACCCAA GGCAATACCT 
GTCAGCCCGA GTGCTTTGGA GGATGATGAC GATGATGACA GCCACACGGT GGTGGCGACA 
GCGCGGGGTA TTTTCAACTC TAATGGTGGG GTCCTGAGCT CCATTGAGAC AGGAGTAAGC 
ATTATTATCC CACAGGGAGC CATCCCTGAT GGCGTGGAGC AAGAGATCTA CTTTAAAGTG 
TGTCGGGATA ACAGCATCCT GCCACCTCTG GACAAAGAGA AAGGAGAAAC TCTGTTGAGT 
CCTCTGGTGA TGTGTGGTCC TCACGGTCTG AAATTCCTGA AGCCAGTGGA GCTCCGCTTA 
CCTCACTGTG CGTCTATGAC CCCTGATGGT TGGTCTTTTG CTCTAAAATC CTCCGACTCC 
TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC AAATCTCTTC CCGGAGATCC CAACTACCTG 
GTTGGAGCCA ACTGTGTGTC TGTGCTTATT GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303252.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301527.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X3, mRNA.

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CloneID	ODa40327
Clone ID Related Accession (Same CDS sequence)	XM_009303252.2 , XM_009303252.3
Accession Version	XM_009303252.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGAGTG AACCTAGAGA TGCCGGCACT GACCAGCGCA GCTCGGGGAT CATACGCCTT 
CACACTATCA AACAGATAAT CGACCGGGAT AAGCATGCAG TGCTGGACAT TACTCCTAAC 
GCAGTGGATC GGCTGAACTA TGCTCAGTGG TATCCCATCG TAGTGTTCTT AAATCCTGAC 
AGCAAGCAGG GTGTGAAGAA CATGAGGACC AGACTGTGTT CCGAGTCCAG GAAAAGTGCC 
AGAAAACTCC ACGAGCGCGC ACTCAAACTG AGGAAGAACA ACCATCATCT CTTCACAGCC 
ACTATTAACA TGAATAACAT GAATGATGGC TGGTATGGAG CACTGAAGGA CACCATTCAG 
CAGCAGCAGA ACCAGCTGGT GTGGGTCTCA GAGGGCAAGG CGGACGGTTC ACATGATGAT 
GACCTGGACC TTCACGATGA CCGTCTGTCC TATTTATCGG CCCCAGGCAG TGAGTACAGT 
ATGTACAGCA CGGACAGCCG CCACACCTCA GATTACGAGG ACACGGACAC CGAGGGTGGC 
GCCTACACTG ACCAGGAACT GGACGAAACC CTGAACGATG AGGTCGGACC ACCCAGCGAA 
CCAGCCATCA CTCGCTCCTC AGAACCTGTC CGCGAAGACC CGCCCGTCAT CCAGGAGCCC 
CCCGGATATG GAGGATATCA GCCCGAACCC ATCAACCGCA TCGACCCCGC GGGGTTCAAG 
GCCCCTGCAC CTCAGCAGAA AGCAGAGGCT GCTCTCCCCT CGCAGCCTGA GCCACTGGCA 
GAGACAGCGC CCCCTGCTGT TCATGTAAAT GTAGGGGCCC CGAGTGCCAA GGATCTCACT 
CAGGGGGAAA TCAGCCCCAA GGCTGGCTCT CTCTTCCCAC CTGCCCCTGA GCTTATCCAA 
CCCCCGTCTC CTGACCCCAA CCAATCCGGA CAGCCCGGGT CAGAGTCCAA GATGTACAAG 
AAGGATATCT ACCGACCCAA CGAACCGGTG CAGATAAGCC AAAACCCTAT GCAGCCCAAC 
TACTACCAGG AAGACAAGCC ATACAGAGAT TATGACCACC AGCCCTACCG CTATGATGGA 
GGCTACATGG AACCAAAGGC TCGTAACTTT GACCCACATT TTGAGGACAG CGTGCCTCCA 
TATAATGATC AATGGGGTCA GTATGACCAC ACCTCTGGGT ATGAACCTCA AACGCTCTAC 
GAGGATGGTC TGAACCGCTT GTATGGTCAC CCTCAGTCAC GAAACGACAC CGGCTACGAC 
ACAGGTCGAC CTCGATATGG CAAACCAACC TCTGGACCAA TCCGACACGA TGAACCACCA 
CCATCACGCC CACAATATGA CACTCAACAA ACCTCTCGTT CCCCAGAACC GCCCAAACAA 
CAATACTACG ACCCTTCCCA AAGGCCTTCC TACACTCAAG CTCCACAAAA AGGCTATATG 
AACTCTGAAG CACCGGTAAC GCCACCCAAA CCTGAGTCGC TCTCACCGCC TTTGGAGCCT 
GCTCTTCCTC CTCCACCTCC TCCAGCCGAA GCAGAGGATG ATCCCGCCAT GAAGCCCCAG 
TCGGTGCTCA CCAGGGTGAA GATGTTCGAG AACAAGCGAT CGGTGTCTGT AGACAGAGCC 
AGAGAGTCTG CAGACTCTGG TATTCGGCCT GTGGACATGG TGCCTAAACC TGGCGCTGCC 
TCCGTGCCCA AAGCCAATTC TTTAAGTAAC CTGGATCAAG AGAAGACCTT TAGAACTCCA 
GAGCCCCAGC AGCCACAGCC TAAAGTAGAT GATATCATTC GTTCAAATAA CAATGACCCT 
GATGAGGATG AAGAATATTA CAGGAAACAA CTGTCCTACT TCGACCGCAG GAGCTTCGAC 
AAACCCTCCA CACAGCTGAC ACCTGCCATC AAACCAGCCC AACCCCAAAC TCAACCAGCA 
CACTATCCCA GAGCAGAGTC AGTGGAGAAA ATCAACCCAG CTGCTCCATC AAACCCAGCT 
GTCCCACCAC CAACCCTGCC TAAACCTGCA GCAACCCCAC CTCTCGACCC TCACGGATCT 
CCCAAAGTAA AGCCTCCTAG TCATGAAGAC ACAGTCCAGA GCACTTACCT GCCTCACAAA 
AGCTTCCCAG AGAAATCCCC CGTCAACGGC ACCGGCGAAC CTCCAAAGAC CAACAGCACT 
GCCACGTCCA GCTACAACCG TTTTGTGCCC AAGCCGTACA CCACCTCCGC AAGACCCTTC 
GAGCGCAAGT TTGACAGTCC CAAGTTCAAT CACAATCTCC TCCCTAATGA CTCCCAAAAC 
AAGACGGATA ATGTGAAAGC TCCCATCACC GCTAAACCAC AGAGCTCACC CCAGCCGGCA 
GACCACGACA GCGGCCTGGA CACCTTCACA CGCACCATGG ACCACAAACC CAAATACCAG 
CAGAACAACA TCAACGCCGT ACCCAAGGCA ATACCTGTCA GCCCGAGTGC TTTGGAGGAT 
GATGACGATG ATGACAGCCA CACGGTGGTG GCGACAGCGC GGGGTATTTT CAACTCTAAT 
GGTGGGGTCC TGAGCTCCAT TGAGACAGGA GTAAGCATTA TTATCCCACA GGGAGCCATC 
CCTGATGGCG TGGAGCAAGA GATCTACTTT AAAGTGTGTC GGGATAACAG CATCCTGCCA 
CCTCTGGACA AAGAGAAAGG AGAAACTCTG TTGAGTCCTC TGGTGATGTG TGGTCCTCAC 
GGTCTGAAAT TCCTGAAGCC AGTGGAGCTC CGCTTACCTC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGATCC GAAGAGTTGG 
CAGAACAAAT CTCTTCCCGG AGATCCCAAC TACCTGGTTG GAGCCAACTG TGTGTCTGTG 
CTTATTGACC ATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301527.1
CDS	410..5587
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303252.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303252.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGAGTG AACCTAGAGA TGCCGGCACT GACCAGCGCA GCTCGGGGAT CATACGCCTT 
CACACTATCA AACAGATAAT CGACCGGGAT AAGCATGCAG TGCTGGACAT TACTCCTAAC 
GCAGTGGATC GGCTGAACTA TGCTCAGTGG TATCCCATCG TAGTGTTCTT AAATCCTGAC 
AGCAAGCAGG GTGTGAAGAA CATGAGGACC AGACTGTGTT CCGAGTCCAG GAAAAGTGCC 
AGAAAACTCC ACGAGCGCGC ACTCAAACTG AGGAAGAACA ACCATCATCT CTTCACAGCC 
ACTATTAACA TGAATAACAT GAATGATGGC TGGTATGGAG CACTGAAGGA CACCATTCAG 
CAGCAGCAGA ACCAGCTGGT GTGGGTCTCA GAGGGCAAGG CGGACGGTTC ACATGATGAT 
GACCTGGACC TTCACGATGA CCGTCTGTCC TATTTATCGG CCCCAGGCAG TGAGTACAGT 
ATGTACAGCA CGGACAGCCG CCACACCTCA GATTACGAGG ACACGGACAC CGAGGGTGGC 
GCCTACACTG ACCAGGAACT GGACGAAACC CTGAACGATG AGGTCGGACC ACCCAGCGAA 
CCAGCCATCA CTCGCTCCTC AGAACCTGTC CGCGAAGACC CGCCCGTCAT CCAGGAGCCC 
CCCGGATATG GAGGATATCA GCCCGAACCC ATCAACCGCA TCGACCCCGC GGGGTTCAAG 
GCCCCTGCAC CTCAGCAGAA AGCAGAGGCT GCTCTCCCCT CGCAGCCTGA GCCACTGGCA 
GAGACAGCGC CCCCTGCTGT TCATGTAAAT GTAGGGGCCC CGAGTGCCAA GGATCTCACT 
CAGGGGGAAA TCAGCCCCAA GGCTGGCTCT CTCTTCCCAC CTGCCCCTGA GCTTATCCAA 
CCCCCGTCTC CTGACCCCAA CCAATCCGGA CAGCCCGGGT CAGAGTCCAA GATGTACAAG 
AAGGATATCT ACCGACCCAA CGAACCGGTG CAGATAAGCC AAAACCCTAT GCAGCCCAAC 
TACTACCAGG AAGACAAGCC ATACAGAGAT TATGACCACC AGCCCTACCG CTATGATGGA 
GGCTACATGG AACCAAAGGC TCGTAACTTT GACCCACATT TTGAGGACAG CGTGCCTCCA 
TATAATGATC AATGGGGTCA GTATGACCAC ACCTCTGGGT ATGAACCTCA AACGCTCTAC 
GAGGATGGTC TGAACCGCTT GTATGGTCAC CCTCAGTCAC GAAACGACAC CGGCTACGAC 
ACAGGTCGAC CTCGATATGG CAAACCAACC TCTGGACCAA TCCGACACGA TGAACCACCA 
CCATCACGCC CACAATATGA CACTCAACAA ACCTCTCGTT CCCCAGAACC GCCCAAACAA 
CAATACTACG ACCCTTCCCA AAGGCCTTCC TACACTCAAG CTCCACAAAA AGGCTATATG 
AACTCTGAAG CACCGGTAAC GCCACCCAAA CCTGAGTCGC TCTCACCGCC TTTGGAGCCT 
GCTCTTCCTC CTCCACCTCC TCCAGCCGAA GCAGAGGATG ATCCCGCCAT GAAGCCCCAG 
TCGGTGCTCA CCAGGGTGAA GATGTTCGAG AACAAGCGAT CGGTGTCTGT AGACAGAGCC 
AGAGAGTCTG CAGACTCTGG TATTCGGCCT GTGGACATGG TGCCTAAACC TGGCGCTGCC 
TCCGTGCCCA AAGCCAATTC TTTAAGTAAC CTGGATCAAG AGAAGACCTT TAGAACTCCA 
GAGCCCCAGC AGCCACAGCC TAAAGTAGAT GATATCATTC GTTCAAATAA CAATGACCCT 
GATGAGGATG AAGAATATTA CAGGAAACAA CTGTCCTACT TCGACCGCAG GAGCTTCGAC 
AAACCCTCCA CACAGCTGAC ACCTGCCATC AAACCAGCCC AACCCCAAAC TCAACCAGCA 
CACTATCCCA GAGCAGAGTC AGTGGAGAAA ATCAACCCAG CTGCTCCATC AAACCCAGCT 
GTCCCACCAC CAACCCTGCC TAAACCTGCA GCAACCCCAC CTCTCGACCC TCACGGATCT 
CCCAAAGTAA AGCCTCCTAG TCATGAAGAC ACAGTCCAGA GCACTTACCT GCCTCACAAA 
AGCTTCCCAG AGAAATCCCC CGTCAACGGC ACCGGCGAAC CTCCAAAGAC CAACAGCACT 
GCCACGTCCA GCTACAACCG TTTTGTGCCC AAGCCGTACA CCACCTCCGC AAGACCCTTC 
GAGCGCAAGT TTGACAGTCC CAAGTTCAAT CACAATCTCC TCCCTAATGA CTCCCAAAAC 
AAGACGGATA ATGTGAAAGC TCCCATCACC GCTAAACCAC AGAGCTCACC CCAGCCGGCA 
GACCACGACA GCGGCCTGGA CACCTTCACA CGCACCATGG ACCACAAACC CAAATACCAG 
CAGAACAACA TCAACGCCGT ACCCAAGGCA ATACCTGTCA GCCCGAGTGC TTTGGAGGAT 
GATGACGATG ATGACAGCCA CACGGTGGTG GCGACAGCGC GGGGTATTTT CAACTCTAAT 
GGTGGGGTCC TGAGCTCCAT TGAGACAGGA GTAAGCATTA TTATCCCACA GGGAGCCATC 
CCTGATGGCG TGGAGCAAGA GATCTACTTT AAAGTGTGTC GGGATAACAG CATCCTGCCA 
CCTCTGGACA AAGAGAAAGG AGAAACTCTG TTGAGTCCTC TGGTGATGTG TGGTCCTCAC 
GGTCTGAAAT TCCTGAAGCC AGTGGAGCTC CGCTTACCTC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGATCC GAAGAGTTGG 
CAGAACAAAT CTCTTCCCGG AGATCCCAAC TACCTGGTTG GAGCCAACTG TGTGTCTGTG 
CTTATTGACC ATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303252.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301527.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X3, mRNA.

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CloneID	ODa40327
Clone ID Related Accession (Same CDS sequence)	XM_009303252.2 , XM_009303252.3
Accession Version	XM_009303252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGAGTG AACCTAGAGA TGCCGGCACT GACCAGCGCA GCTCGGGGAT CATACGCCTT 
CACACTATCA AACAGATAAT CGACCGGGAT AAGCATGCAG TGCTGGACAT TACTCCTAAC 
GCAGTGGATC GGCTGAACTA TGCTCAGTGG TATCCCATCG TAGTGTTCTT AAATCCTGAC 
AGCAAGCAGG GTGTGAAGAA CATGAGGACC AGACTGTGTT CCGAGTCCAG GAAAAGTGCC 
AGAAAACTCC ACGAGCGCGC ACTCAAACTG AGGAAGAACA ACCATCATCT CTTCACAGCC 
ACTATTAACA TGAATAACAT GAATGATGGC TGGTATGGAG CACTGAAGGA CACCATTCAG 
CAGCAGCAGA ACCAGCTGGT GTGGGTCTCA GAGGGCAAGG CGGACGGTTC ACATGATGAT 
GACCTGGACC TTCACGATGA CCGTCTGTCC TATTTATCGG CCCCAGGCAG TGAGTACAGT 
ATGTACAGCA CGGACAGCCG CCACACCTCA GATTACGAGG ACACGGACAC CGAGGGTGGC 
GCCTACACTG ACCAGGAACT GGACGAAACC CTGAACGATG AGGTCGGACC ACCCAGCGAA 
CCAGCCATCA CTCGCTCCTC AGAACCTGTC CGCGAAGACC CGCCCGTCAT CCAGGAGCCC 
CCCGGATATG GAGGATATCA GCCCGAACCC ATCAACCGCA TCGACCCCGC GGGGTTCAAG 
GCCCCTGCAC CTCAGCAGAA AGCAGAGGCT GCTCTCCCCT CGCAGCCTGA GCCACTGGCA 
GAGACAGCGC CCCCTGCTGT TCATGTAAAT GTAGGGGCCC CGAGTGCCAA GGATCTCACT 
CAGGGGGAAA TCAGCCCCAA GGCTGGCTCT CTCTTCCCAC CTGCCCCTGA GCTTATCCAA 
CCCCCGTCTC CTGACCCCAA CCAATCCGGA CAGCCCGGGT CAGAGTCCAA GATGTACAAG 
AAGGATATCT ACCGACCCAA CGAACCGGTG CAGATAAGCC AAAACCCTAT GCAGCCCAAC 
TACTACCAGG AAGACAAGCC ATACAGAGAT TATGACCACC AGCCCTACCG CTATGATGGA 
GGCTACATGG AACCAAAGGC TCGTAACTTT GACCCACATT TTGAGGACAG CGTGCCTCCA 
TATAATGATC AATGGGGTCA GTATGACCAC ACCTCTGGGT ATGAACCTCA AACGCTCTAC 
GAGGATGGTC TGAACCGCTT GTATGGTCAC CCTCAGTCAC GAAACGACAC CGGCTACGAC 
ACAGGTCGAC CTCGATATGG CAAACCAACC TCTGGACCAA TCCGACACGA TGAACCACCA 
CCATCACGCC CACAATATGA CACTCAACAA ACCTCTCGTT CCCCAGAACC GCCCAAACAA 
CAATACTACG ACCCTTCCCA AAGGCCTTCC TACACTCAAG CTCCACAAAA AGGCTATATG 
AACTCTGAAG CACCGGTAAC GCCACCCAAA CCTGAGTCGC TCTCACCGCC TTTGGAGCCT 
GCTCTTCCTC CTCCACCTCC TCCAGCCGAA GCAGAGGATG ATCCCGCCAT GAAGCCCCAG 
TCGGTGCTCA CCAGGGTGAA GATGTTCGAG AACAAGCGAT CGGTGTCTGT AGACAGAGCC 
AGAGAGTCTG CAGACTCTGG TATTCGGCCT GTGGACATGG TGCCTAAACC TGGCGCTGCC 
TCCGTGCCCA AAGCCAATTC TTTAAGTAAC CTGGATCAAG AGAAGACCTT TAGAACTCCA 
GAGCCCCAGC AGCCACAGCC TAAAGTAGAT GATATCATTC GTTCAAATAA CAATGACCCT 
GATGAGGATG AAGAATATTA CAGGAAACAA CTGTCCTACT TCGACCGCAG GAGCTTCGAC 
AAACCCTCCA CACAGCTGAC ACCTGCCATC AAACCAGCCC AACCCCAAAC TCAACCAGCA 
CACTATCCCA GAGCAGAGTC AGTGGAGAAA ATCAACCCAG CTGCTCCATC AAACCCAGCT 
GTCCCACCAC CAACCCTGCC TAAACCTGCA GCAACCCCAC CTCTCGACCC TCACGGATCT 
CCCAAAGTAA AGCCTCCTAG TCATGAAGAC ACAGTCCAGA GCACTTACCT GCCTCACAAA 
AGCTTCCCAG AGAAATCCCC CGTCAACGGC ACCGGCGAAC CTCCAAAGAC CAACAGCACT 
GCCACGTCCA GCTACAACCG TTTTGTGCCC AAGCCGTACA CCACCTCCGC AAGACCCTTC 
GAGCGCAAGT TTGACAGTCC CAAGTTCAAT CACAATCTCC TCCCTAATGA CTCCCAAAAC 
AAGACGGATA ATGTGAAAGC TCCCATCACC GCTAAACCAC AGAGCTCACC CCAGCCGGCA 
GACCACGACA GCGGCCTGGA CACCTTCACA CGCACCATGG ACCACAAACC CAAATACCAG 
CAGAACAACA TCAACGCCGT ACCCAAGGCA ATACCTGTCA GCCCGAGTGC TTTGGAGGAT 
GATGACGATG ATGACAGCCA CACGGTGGTG GCGACAGCGC GGGGTATTTT CAACTCTAAT 
GGTGGGGTCC TGAGCTCCAT TGAGACAGGA GTAAGCATTA TTATCCCACA GGGAGCCATC 
CCTGATGGCG TGGAGCAAGA GATCTACTTT AAAGTGTGTC GGGATAACAG CATCCTGCCA 
CCTCTGGACA AAGAGAAAGG AGAAACTCTG TTGAGTCCTC TGGTGATGTG TGGTCCTCAC 
GGTCTGAAAT TCCTGAAGCC AGTGGAGCTC CGCTTACCTC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGATCC GAAGAGTTGG 
CAGAACAAAT CTCTTCCCGG AGATCCCAAC TACCTGGTTG GAGCCAACTG TGTGTCTGTG 
CTTATTGACC ATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301527.1
CDS	384..5561
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303252.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303252.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGAGTG AACCTAGAGA TGCCGGCACT GACCAGCGCA GCTCGGGGAT CATACGCCTT 
CACACTATCA AACAGATAAT CGACCGGGAT AAGCATGCAG TGCTGGACAT TACTCCTAAC 
GCAGTGGATC GGCTGAACTA TGCTCAGTGG TATCCCATCG TAGTGTTCTT AAATCCTGAC 
AGCAAGCAGG GTGTGAAGAA CATGAGGACC AGACTGTGTT CCGAGTCCAG GAAAAGTGCC 
AGAAAACTCC ACGAGCGCGC ACTCAAACTG AGGAAGAACA ACCATCATCT CTTCACAGCC 
ACTATTAACA TGAATAACAT GAATGATGGC TGGTATGGAG CACTGAAGGA CACCATTCAG 
CAGCAGCAGA ACCAGCTGGT GTGGGTCTCA GAGGGCAAGG CGGACGGTTC ACATGATGAT 
GACCTGGACC TTCACGATGA CCGTCTGTCC TATTTATCGG CCCCAGGCAG TGAGTACAGT 
ATGTACAGCA CGGACAGCCG CCACACCTCA GATTACGAGG ACACGGACAC CGAGGGTGGC 
GCCTACACTG ACCAGGAACT GGACGAAACC CTGAACGATG AGGTCGGACC ACCCAGCGAA 
CCAGCCATCA CTCGCTCCTC AGAACCTGTC CGCGAAGACC CGCCCGTCAT CCAGGAGCCC 
CCCGGATATG GAGGATATCA GCCCGAACCC ATCAACCGCA TCGACCCCGC GGGGTTCAAG 
GCCCCTGCAC CTCAGCAGAA AGCAGAGGCT GCTCTCCCCT CGCAGCCTGA GCCACTGGCA 
GAGACAGCGC CCCCTGCTGT TCATGTAAAT GTAGGGGCCC CGAGTGCCAA GGATCTCACT 
CAGGGGGAAA TCAGCCCCAA GGCTGGCTCT CTCTTCCCAC CTGCCCCTGA GCTTATCCAA 
CCCCCGTCTC CTGACCCCAA CCAATCCGGA CAGCCCGGGT CAGAGTCCAA GATGTACAAG 
AAGGATATCT ACCGACCCAA CGAACCGGTG CAGATAAGCC AAAACCCTAT GCAGCCCAAC 
TACTACCAGG AAGACAAGCC ATACAGAGAT TATGACCACC AGCCCTACCG CTATGATGGA 
GGCTACATGG AACCAAAGGC TCGTAACTTT GACCCACATT TTGAGGACAG CGTGCCTCCA 
TATAATGATC AATGGGGTCA GTATGACCAC ACCTCTGGGT ATGAACCTCA AACGCTCTAC 
GAGGATGGTC TGAACCGCTT GTATGGTCAC CCTCAGTCAC GAAACGACAC CGGCTACGAC 
ACAGGTCGAC CTCGATATGG CAAACCAACC TCTGGACCAA TCCGACACGA TGAACCACCA 
CCATCACGCC CACAATATGA CACTCAACAA ACCTCTCGTT CCCCAGAACC GCCCAAACAA 
CAATACTACG ACCCTTCCCA AAGGCCTTCC TACACTCAAG CTCCACAAAA AGGCTATATG 
AACTCTGAAG CACCGGTAAC GCCACCCAAA CCTGAGTCGC TCTCACCGCC TTTGGAGCCT 
GCTCTTCCTC CTCCACCTCC TCCAGCCGAA GCAGAGGATG ATCCCGCCAT GAAGCCCCAG 
TCGGTGCTCA CCAGGGTGAA GATGTTCGAG AACAAGCGAT CGGTGTCTGT AGACAGAGCC 
AGAGAGTCTG CAGACTCTGG TATTCGGCCT GTGGACATGG TGCCTAAACC TGGCGCTGCC 
TCCGTGCCCA AAGCCAATTC TTTAAGTAAC CTGGATCAAG AGAAGACCTT TAGAACTCCA 
GAGCCCCAGC AGCCACAGCC TAAAGTAGAT GATATCATTC GTTCAAATAA CAATGACCCT 
GATGAGGATG AAGAATATTA CAGGAAACAA CTGTCCTACT TCGACCGCAG GAGCTTCGAC 
AAACCCTCCA CACAGCTGAC ACCTGCCATC AAACCAGCCC AACCCCAAAC TCAACCAGCA 
CACTATCCCA GAGCAGAGTC AGTGGAGAAA ATCAACCCAG CTGCTCCATC AAACCCAGCT 
GTCCCACCAC CAACCCTGCC TAAACCTGCA GCAACCCCAC CTCTCGACCC TCACGGATCT 
CCCAAAGTAA AGCCTCCTAG TCATGAAGAC ACAGTCCAGA GCACTTACCT GCCTCACAAA 
AGCTTCCCAG AGAAATCCCC CGTCAACGGC ACCGGCGAAC CTCCAAAGAC CAACAGCACT 
GCCACGTCCA GCTACAACCG TTTTGTGCCC AAGCCGTACA CCACCTCCGC AAGACCCTTC 
GAGCGCAAGT TTGACAGTCC CAAGTTCAAT CACAATCTCC TCCCTAATGA CTCCCAAAAC 
AAGACGGATA ATGTGAAAGC TCCCATCACC GCTAAACCAC AGAGCTCACC CCAGCCGGCA 
GACCACGACA GCGGCCTGGA CACCTTCACA CGCACCATGG ACCACAAACC CAAATACCAG 
CAGAACAACA TCAACGCCGT ACCCAAGGCA ATACCTGTCA GCCCGAGTGC TTTGGAGGAT 
GATGACGATG ATGACAGCCA CACGGTGGTG GCGACAGCGC GGGGTATTTT CAACTCTAAT 
GGTGGGGTCC TGAGCTCCAT TGAGACAGGA GTAAGCATTA TTATCCCACA GGGAGCCATC 
CCTGATGGCG TGGAGCAAGA GATCTACTTT AAAGTGTGTC GGGATAACAG CATCCTGCCA 
CCTCTGGACA AAGAGAAAGG AGAAACTCTG TTGAGTCCTC TGGTGATGTG TGGTCCTCAC 
GGTCTGAAAT TCCTGAAGCC AGTGGAGCTC CGCTTACCTC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGATCC GAAGAGTTGG 
CAGAACAAAT CTCTTCCCGG AGATCCCAAC TACCTGGTTG GAGCCAACTG TGTGTCTGTG 
CTTATTGACC ATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303253.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301528.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X5, mRNA.

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CloneID	ODa40328
Clone ID Related Accession (Same CDS sequence)	XM_009303253.3 , XM_009303253.2
Accession Version	XM_009303253.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303253.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301528.1
CDS	384..5549
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303253.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303253.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303253.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301528.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X5, mRNA.

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CloneID	ODa40328
Clone ID Related Accession (Same CDS sequence)	XM_009303253.3 , XM_009303253.2
Accession Version	XM_009303253.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301528.1
CDS	409..5574
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303253.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303253.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGCGTCTAT GACCCCTGAT GGTGATCCGA AGAGTTGGCA GAACAAATCT 
CTTCCCGGAG ATCCCAACTA CCTGGTTGGA GCCAACTGTG TGTCTGTGCT TATTGACCAT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303254.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301529.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X6, mRNA.

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CloneID	ODa40329
Clone ID Related Accession (Same CDS sequence)	XM_009303254.2 , XM_009303254.3
Accession Version	XM_009303254.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301529.1
CDS	410..5554
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303254.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303254.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303254.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301529.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa40329
Clone ID Related Accession (Same CDS sequence)	XM_009303254.2 , XM_009303254.3
Accession Version	XM_009303254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301529.1
CDS	384..5528
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303254.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303254.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGAAAGC AGAGGCTGCT 
CTCCCCTCGC AGCCTGAGCC ACTGGCAGAG ACAGCGCCCC CTGCTGTTCA TGTAAATGTA 
GGGGCCCCGA GTGCCAAGGA TCTCACTCAG GGGGAAATCA GCCCCAAGGC TGGCTCTCTC 
TTCCCACCTG CCCCTGAGCT TATCCAACCC CCGTCTCCTG ACCCCAACCA ATCCGGACAG 
CCCGGGTCAG AGTCCAAGAT GTACAAGAAG GATATCTACC GACCCAACGA ACCGGTGCAG 
ATAAGCCAAA ACCCTATGCA GCCCAACTAC TACCAGGAAG ACAAGCCATA CAGAGATTAT 
GACCACCAGC CCTACCGCTA TGATGGAGGC TACATGGAAC CAAAGGCTCG TAACTTTGAC 
CCACATTTTG AGGACAGCGT GCCTCCATAT AATGATCAAT GGGGTCAGTA TGACCACACC 
TCTGGGTATG AACCTCAAAC GCTCTACGAG GATGGTCTGA ACCGCTTGTA TGGTCACCCT 
CAGTCACGAA ACGACACCGG CTACGACACA GGTCGACCTC GATATGGCAA ACCAACCTCT 
GGACCAATCC GACACGATGA ACCACCACCA TCACGCCCAC AATATGACAC TCAACAAACC 
TCTCGTTCCC CAGAACCGCC CAAACAACAA TACTACGACC CTTCCCAAAG GCCTTCCTAC 
ACTCAAGCTC CACAAAAAGG CTATATGAAC TCTGAAGCAC CGGTAACGCC ACCCAAACCT 
GAGTCGCTCT CACCGCCTTT GGAGCCTGCT CTTCCTCCTC CACCTCCTCC AGCCGAAGCA 
GAGGATGATC CCGCCATGAA GCCCCAGTCG GTGCTCACCA GGGTGAAGAT GTTCGAGAAC 
AAGCGATCGG TGTCTGTAGA CAGAGCCAGA GAGTCTGCAG ACTCTGGTAT TCGGCCTGTG 
GACATGGTGC CTAAACCTGG CGCTGCCTCC GTGCCCAAAG CCAATTCTTT AAGTAACCTG 
GATCAAGAGA AGACCTTTAG AACTCCAGAG CCCCAGCAGC CACAGCCTAA AGTAGATGAT 
ATCATTCGTT CAAATAACAA TGACCCTGAT GAGGATGAAG AATATTACAG GAAACAACTG 
TCCTACTTCG ACCGCAGGAG CTTCGACAAA CCCTCCACAC AGCTGACACC TGCCATCAAA 
CCAGCCCAAC CCCAAACTCA ACCAGCACAC TATCCCAGAG CAGAGTCAGT GGAGAAAATC 
AACCCAGCTG CTCCATCAAA CCCAGCTGTC CCACCACCAA CCCTGCCTAA ACCTGCAGCA 
ACCCCACCTC TCGACCCTCA CGGATCTCCC AAAGTAAAGC CTCCTAGTCA TGAAGACACA 
GTCCAGAGCA CTTACCTGCC TCACAAAAGC TTCCCAGAGA AATCCCCCGT CAACGGCACC 
GGCGAACCTC CAAAGACCAA CAGCACTGCC ACGTCCAGCT ACAACCGTTT TGTGCCCAAG 
CCGTACACCA CCTCCGCAAG ACCCTTCGAG CGCAAGTTTG ACAGTCCCAA GTTCAATCAC 
AATCTCCTCC CTAATGACTC CCAAAACAAG ACGGATAATG TGAAAGCTCC CATCACCGCT 
AAACCACAGA GCTCACCCCA GCCGGCAGAC CACGACAGCG GCCTGGACAC CTTCACACGC 
ACCATGGACC ACAAACCCAA ATACCAGCAG AACAACATCA ACGCCGTACC CAAGGCAATA 
CCTGTCAGCC CGAGTGCTTT GGAGGATGAT GACGATGATG ACAGCCACAC GGTGGTGGCG 
ACAGCGCGGG GTATTTTCAA CTCTAATGGT GGGGTCCTGA GCTCCATTGA GACAGGAGTA 
AGCATTATTA TCCCACAGGG AGCCATCCCT GATGGCGTGG AGCAAGAGAT CTACTTTAAA 
GTGTGTCGGG ATAACAGCAT CCTGCCACCT CTGGACAAAG AGAAAGGAGA AACTCTGTTG 
AGTCCTCTGG TGATGTGTGG TCCTCACGGT CTGAAATTCC TGAAGCCAGT GGAGCTCCGC 
TTACCTCACT GTGATCCGAA GAGTTGGCAG AACAAATCTC TTCCCGGAGA TCCCAACTAC 
CTGGTTGGAG CCAACTGTGT GTCTGTGCTT ATTGACCATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303255.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301530.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa40330
Clone ID Related Accession (Same CDS sequence)	XM_009303255.2 , XM_009303255.3
Accession Version	XM_009303255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGATGTA CAAGAAGGAT 
ATCTACCGAC CCAACGAACC GGTGCAGATA AGCCAAAACC CTATGCAGCC CAACTACTAC 
CAGGAAGACA AGCCATACAG AGATTATGAC CACCAGCCCT ACCGCTATGA TGGAGGCTAC 
ATGGAACCAA AGGCTCGTAA CTTTGACCCA CATTTTGAGG ACAGCGTGCC TCCATATAAT 
GATCAATGGG GTCAGTATGA CCACACCTCT GGGTATGAAC CTCAAACGCT CTACGAGGAT 
GGTCTGAACC GCTTGTATGG TCACCCTCAG TCACGAAACG ACACCGGCTA CGACACAGGT 
CGACCTCGAT ATGGCAAACC AACCTCTGGA CCAATCCGAC ACGATGAACC ACCACCATCA 
CGCCCACAAT ATGACACTCA ACAAACCTCT CGTTCCCCAG AACCGCCCAA ACAACAATAC 
TACGACCCTT CCCAAAGGCC TTCCTACACT CAAGCTCCAC AAAAAGGCTA TATGAACTCT 
GAAGCACCGG TAACGCCACC CAAACCTGAG TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT 
CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG GATGATCCCG CCATGAAGCC CCAGTCGGTG 
CTCACCAGGG TGAAGATGTT CGAGAACAAG CGATCGGTGT CTGTAGACAG AGCCAGAGAG 
TCTGCAGACT CTGGTATTCG GCCTGTGGAC ATGGTGCCTA AACCTGGCGC TGCCTCCGTG 
CCCAAAGCCA ATTCTTTAAG TAACCTGGAT CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC 
CAGCAGCCAC AGCCTAAAGT AGATGATATC ATTCGTTCAA ATAACAATGA CCCTGATGAG 
GATGAAGAAT ATTACAGGAA ACAACTGTCC TACTTCGACC GCAGGAGCTT CGACAAACCC 
TCCACACAGC TGACACCTGC CATCAAACCA GCCCAACCCC AAACTCAACC AGCACACTAT 
CCCAGAGCAG AGTCAGTGGA GAAAATCAAC CCAGCTGCTC CATCAAACCC AGCTGTCCCA 
CCACCAACCC TGCCTAAACC TGCAGCAACC CCACCTCTCG ACCCTCACGG ATCTCCCAAA 
GTAAAGCCTC CTAGTCATGA AGACACAGTC CAGAGCACTT ACCTGCCTCA CAAAAGCTTC 
CCAGAGAAAT CCCCCGTCAA CGGCACCGGC GAACCTCCAA AGACCAACAG CACTGCCACG 
TCCAGCTACA ACCGTTTTGT GCCCAAGCCG TACACCACCT CCGCAAGACC CTTCGAGCGC 
AAGTTTGACA GTCCCAAGTT CAATCACAAT CTCCTCCCTA ATGACTCCCA AAACAAGACG 
GATAATGTGA AAGCTCCCAT CACCGCTAAA CCACAGAGCT CACCCCAGCC GGCAGACCAC 
GACAGCGGCC TGGACACCTT CACACGCACC ATGGACCACA AACCCAAATA CCAGCAGAAC 
AACATCAACG CCGTACCCAA GGCAATACCT GTCAGCCCGA GTGCTTTGGA GGATGATGAC 
GATGATGACA GCCACACGGT GGTGGCGACA GCGCGGGGTA TTTTCAACTC TAATGGTGGG 
GTCCTGAGCT CCATTGAGAC AGGAGTAAGC ATTATTATCC CACAGGGAGC CATCCCTGAT 
GGCGTGGAGC AAGAGATCTA CTTTAAAGTG TGTCGGGATA ACAGCATCCT GCCACCTCTG 
GACAAAGAGA AAGGAGAAAC TCTGTTGAGT CCTCTGGTGA TGTGTGGTCC TCACGGTCTG 
AAATTCCTGA AGCCAGTGGA GCTCCGCTTA CCTCACTGTG CGTCTATGAC CCCTGATGGT 
TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC 
AAATCTCTTC CCGGAGATCC CAACTACCTG GTTGGAGCCA ACTGTGTGTC TGTGCTTATT 
GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301530.1
CDS	410..5401
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303255.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303255.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGATGTA CAAGAAGGAT 
ATCTACCGAC CCAACGAACC GGTGCAGATA AGCCAAAACC CTATGCAGCC CAACTACTAC 
CAGGAAGACA AGCCATACAG AGATTATGAC CACCAGCCCT ACCGCTATGA TGGAGGCTAC 
ATGGAACCAA AGGCTCGTAA CTTTGACCCA CATTTTGAGG ACAGCGTGCC TCCATATAAT 
GATCAATGGG GTCAGTATGA CCACACCTCT GGGTATGAAC CTCAAACGCT CTACGAGGAT 
GGTCTGAACC GCTTGTATGG TCACCCTCAG TCACGAAACG ACACCGGCTA CGACACAGGT 
CGACCTCGAT ATGGCAAACC AACCTCTGGA CCAATCCGAC ACGATGAACC ACCACCATCA 
CGCCCACAAT ATGACACTCA ACAAACCTCT CGTTCCCCAG AACCGCCCAA ACAACAATAC 
TACGACCCTT CCCAAAGGCC TTCCTACACT CAAGCTCCAC AAAAAGGCTA TATGAACTCT 
GAAGCACCGG TAACGCCACC CAAACCTGAG TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT 
CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG GATGATCCCG CCATGAAGCC CCAGTCGGTG 
CTCACCAGGG TGAAGATGTT CGAGAACAAG CGATCGGTGT CTGTAGACAG AGCCAGAGAG 
TCTGCAGACT CTGGTATTCG GCCTGTGGAC ATGGTGCCTA AACCTGGCGC TGCCTCCGTG 
CCCAAAGCCA ATTCTTTAAG TAACCTGGAT CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC 
CAGCAGCCAC AGCCTAAAGT AGATGATATC ATTCGTTCAA ATAACAATGA CCCTGATGAG 
GATGAAGAAT ATTACAGGAA ACAACTGTCC TACTTCGACC GCAGGAGCTT CGACAAACCC 
TCCACACAGC TGACACCTGC CATCAAACCA GCCCAACCCC AAACTCAACC AGCACACTAT 
CCCAGAGCAG AGTCAGTGGA GAAAATCAAC CCAGCTGCTC CATCAAACCC AGCTGTCCCA 
CCACCAACCC TGCCTAAACC TGCAGCAACC CCACCTCTCG ACCCTCACGG ATCTCCCAAA 
GTAAAGCCTC CTAGTCATGA AGACACAGTC CAGAGCACTT ACCTGCCTCA CAAAAGCTTC 
CCAGAGAAAT CCCCCGTCAA CGGCACCGGC GAACCTCCAA AGACCAACAG CACTGCCACG 
TCCAGCTACA ACCGTTTTGT GCCCAAGCCG TACACCACCT CCGCAAGACC CTTCGAGCGC 
AAGTTTGACA GTCCCAAGTT CAATCACAAT CTCCTCCCTA ATGACTCCCA AAACAAGACG 
GATAATGTGA AAGCTCCCAT CACCGCTAAA CCACAGAGCT CACCCCAGCC GGCAGACCAC 
GACAGCGGCC TGGACACCTT CACACGCACC ATGGACCACA AACCCAAATA CCAGCAGAAC 
AACATCAACG CCGTACCCAA GGCAATACCT GTCAGCCCGA GTGCTTTGGA GGATGATGAC 
GATGATGACA GCCACACGGT GGTGGCGACA GCGCGGGGTA TTTTCAACTC TAATGGTGGG 
GTCCTGAGCT CCATTGAGAC AGGAGTAAGC ATTATTATCC CACAGGGAGC CATCCCTGAT 
GGCGTGGAGC AAGAGATCTA CTTTAAAGTG TGTCGGGATA ACAGCATCCT GCCACCTCTG 
GACAAAGAGA AAGGAGAAAC TCTGTTGAGT CCTCTGGTGA TGTGTGGTCC TCACGGTCTG 
AAATTCCTGA AGCCAGTGGA GCTCCGCTTA CCTCACTGTG CGTCTATGAC CCCTGATGGT 
TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC 
AAATCTCTTC CCGGAGATCC CAACTACCTG GTTGGAGCCA ACTGTGTGTC TGTGCTTATT 
GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303255.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301530.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X9, mRNA.

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CloneID	ODa40330
Clone ID Related Accession (Same CDS sequence)	XM_009303255.2 , XM_009303255.3
Accession Version	XM_009303255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGATGTA CAAGAAGGAT 
ATCTACCGAC CCAACGAACC GGTGCAGATA AGCCAAAACC CTATGCAGCC CAACTACTAC 
CAGGAAGACA AGCCATACAG AGATTATGAC CACCAGCCCT ACCGCTATGA TGGAGGCTAC 
ATGGAACCAA AGGCTCGTAA CTTTGACCCA CATTTTGAGG ACAGCGTGCC TCCATATAAT 
GATCAATGGG GTCAGTATGA CCACACCTCT GGGTATGAAC CTCAAACGCT CTACGAGGAT 
GGTCTGAACC GCTTGTATGG TCACCCTCAG TCACGAAACG ACACCGGCTA CGACACAGGT 
CGACCTCGAT ATGGCAAACC AACCTCTGGA CCAATCCGAC ACGATGAACC ACCACCATCA 
CGCCCACAAT ATGACACTCA ACAAACCTCT CGTTCCCCAG AACCGCCCAA ACAACAATAC 
TACGACCCTT CCCAAAGGCC TTCCTACACT CAAGCTCCAC AAAAAGGCTA TATGAACTCT 
GAAGCACCGG TAACGCCACC CAAACCTGAG TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT 
CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG GATGATCCCG CCATGAAGCC CCAGTCGGTG 
CTCACCAGGG TGAAGATGTT CGAGAACAAG CGATCGGTGT CTGTAGACAG AGCCAGAGAG 
TCTGCAGACT CTGGTATTCG GCCTGTGGAC ATGGTGCCTA AACCTGGCGC TGCCTCCGTG 
CCCAAAGCCA ATTCTTTAAG TAACCTGGAT CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC 
CAGCAGCCAC AGCCTAAAGT AGATGATATC ATTCGTTCAA ATAACAATGA CCCTGATGAG 
GATGAAGAAT ATTACAGGAA ACAACTGTCC TACTTCGACC GCAGGAGCTT CGACAAACCC 
TCCACACAGC TGACACCTGC CATCAAACCA GCCCAACCCC AAACTCAACC AGCACACTAT 
CCCAGAGCAG AGTCAGTGGA GAAAATCAAC CCAGCTGCTC CATCAAACCC AGCTGTCCCA 
CCACCAACCC TGCCTAAACC TGCAGCAACC CCACCTCTCG ACCCTCACGG ATCTCCCAAA 
GTAAAGCCTC CTAGTCATGA AGACACAGTC CAGAGCACTT ACCTGCCTCA CAAAAGCTTC 
CCAGAGAAAT CCCCCGTCAA CGGCACCGGC GAACCTCCAA AGACCAACAG CACTGCCACG 
TCCAGCTACA ACCGTTTTGT GCCCAAGCCG TACACCACCT CCGCAAGACC CTTCGAGCGC 
AAGTTTGACA GTCCCAAGTT CAATCACAAT CTCCTCCCTA ATGACTCCCA AAACAAGACG 
GATAATGTGA AAGCTCCCAT CACCGCTAAA CCACAGAGCT CACCCCAGCC GGCAGACCAC 
GACAGCGGCC TGGACACCTT CACACGCACC ATGGACCACA AACCCAAATA CCAGCAGAAC 
AACATCAACG CCGTACCCAA GGCAATACCT GTCAGCCCGA GTGCTTTGGA GGATGATGAC 
GATGATGACA GCCACACGGT GGTGGCGACA GCGCGGGGTA TTTTCAACTC TAATGGTGGG 
GTCCTGAGCT CCATTGAGAC AGGAGTAAGC ATTATTATCC CACAGGGAGC CATCCCTGAT 
GGCGTGGAGC AAGAGATCTA CTTTAAAGTG TGTCGGGATA ACAGCATCCT GCCACCTCTG 
GACAAAGAGA AAGGAGAAAC TCTGTTGAGT CCTCTGGTGA TGTGTGGTCC TCACGGTCTG 
AAATTCCTGA AGCCAGTGGA GCTCCGCTTA CCTCACTGTG CGTCTATGAC CCCTGATGGT 
TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC 
AAATCTCTTC CCGGAGATCC CAACTACCTG GTTGGAGCCA ACTGTGTGTC TGTGCTTATT 
GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301530.1
CDS	384..5375
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303255.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303255.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGGCT GACGGGCTCT 
TTTCCCACCA GAAAGCTCCC GCTCCATAGA AGTCGAGATG AGGAGCGCAT CTCTAAACCA 
GCGGCTCTAT CCACTCCAGT TAAGATGGCT GAGGATGCTC TTCTGGCTCC GCCCAGCGAA 
CAGCCCGGGG ACAAACAGAT CCCTCCACTG CCAGAGCCGA AACCAGTGTA TGCACAACCT 
GGGCAACCCG ATGTAGATCT GCCAGTGAGC CCGTCTGATG CCCCTGTCCC CAGCGTCACC 
CATGATGACA GCATCCTCCG CAGGCCCAGT ATGAAGCTGG TGAAGTTTAA GAAAGGGGAA 
AGTGTCGGAT TGAGGCTGGC TGGAGGAAAC GATGTGGGGA TCTTTGTGGC TGGAGTTCTG 
GAGGACAGTC CTGCTGCTAA GGAAGGCCTG GAGGAGGGAG ACCAGATACT CAGGGTTAAC 
AATGTTGACT TTGCAAACAT TATTCGTGAG GAAGCAGTTC TGTTCCTGCT AGATCTGCCC 
AGAGGTGAGG AGGTCACCAT CCTTGCGCAG AAGAAGAAAG ATGTGTACCG GCGAATAGTG 
GAGTCTGATG TGGGCGACTC CTTCTACATC AGGACACACT TTGAGTACGA GAAAGAGTCT 
CCTTATGGGC TGAGCTTTAA TAAAGGAGAG GTGTTCAGAG TGGTGGACAC TCTCTACAAT 
GGCAAACTGG GCTCCTGGCT GGCTATCCGC ATCGGGAAGA ACCACCAGGA GGTGGAAAGA 
GGCATCATTC CCAACAAGAA CAGGGCGGAA CAGTTATCAA GCGTACAGTA CACACTCCCC 
AAGACTGCAG GTGGTGATCG TGCTGATTTC TGGAGGTTCA GAGGCCTGCG CAGTTCAAAG 
AGGAACTTGC GCAAGAGTAG AGAAGATCTG TCAGCTCAGC CCGTTCAGAC CAAGTTCCCT 
GCTTATGAAA GGGTGGTTCT TAGAGAGGCT GGTTTCCTGA GACCTATTGT GATCTTTGGA 
CCAATCGCAG ATGTGGCACG AGACAAACTG GCAAGAGAGG TGCCTGACCT ATTCGAGCTC 
GCAAAGACAC AGCTCCCAGA TGGAAGGGAA AAGAGTGAAC CTAGAGATGC CGGCACTGAC 
CAGCGCAGCT CGGGGATCAT ACGCCTTCAC ACTATCAAAC AGATAATCGA CCGGGATAAG 
CATGCAGTGC TGGACATTAC TCCTAACGCA GTGGATCGGC TGAACTATGC TCAGTGGTAT 
CCCATCGTAG TGTTCTTAAA TCCTGACAGC AAGCAGGGTG TGAAGAACAT GAGGACCAGA 
CTGTGTTCCG AGTCCAGGAA AAGTGCCAGA AAACTCCACG AGCGCGCACT CAAACTGAGG 
AAGAACAACC ATCATCTCTT CACAGCCACT ATTAACATGA ATAACATGAA TGATGGCTGG 
TATGGAGCAC TGAAGGACAC CATTCAGCAG CAGCAGAACC AGCTGGTGTG GGTCTCAGAG 
GGCAAGGCGG ACGGTTCACA TGATGATGAC CTGGACCTTC ACGATGACCG TCTGTCCTAT 
TTATCGGCCC CAGGCAGTGA GTACAGTATG TACAGCACGG ACAGCCGCCA CACCTCAGAT 
TACGAGGACA CGGACACCGA GGGTGGCGCC TACACTGACC AGGAACTGGA CGAAACCCTG 
AACGATGAGG TCGGACCACC CAGCGAACCA GCCATCACTC GCTCCTCAGA ACCTGTCCGC 
GAAGACCCGC CCGTCATCCA GGAGCCCCCC GGATATGGAG GATATCAGCC CGAACCCATC 
AACCGCATCG ACCCCGCGGG GTTCAAGGCC CCTGCACCTC AGCAGATGTA CAAGAAGGAT 
ATCTACCGAC CCAACGAACC GGTGCAGATA AGCCAAAACC CTATGCAGCC CAACTACTAC 
CAGGAAGACA AGCCATACAG AGATTATGAC CACCAGCCCT ACCGCTATGA TGGAGGCTAC 
ATGGAACCAA AGGCTCGTAA CTTTGACCCA CATTTTGAGG ACAGCGTGCC TCCATATAAT 
GATCAATGGG GTCAGTATGA CCACACCTCT GGGTATGAAC CTCAAACGCT CTACGAGGAT 
GGTCTGAACC GCTTGTATGG TCACCCTCAG TCACGAAACG ACACCGGCTA CGACACAGGT 
CGACCTCGAT ATGGCAAACC AACCTCTGGA CCAATCCGAC ACGATGAACC ACCACCATCA 
CGCCCACAAT ATGACACTCA ACAAACCTCT CGTTCCCCAG AACCGCCCAA ACAACAATAC 
TACGACCCTT CCCAAAGGCC TTCCTACACT CAAGCTCCAC AAAAAGGCTA TATGAACTCT 
GAAGCACCGG TAACGCCACC CAAACCTGAG TCGCTCTCAC CGCCTTTGGA GCCTGCTCTT 
CCTCCTCCAC CTCCTCCAGC CGAAGCAGAG GATGATCCCG CCATGAAGCC CCAGTCGGTG 
CTCACCAGGG TGAAGATGTT CGAGAACAAG CGATCGGTGT CTGTAGACAG AGCCAGAGAG 
TCTGCAGACT CTGGTATTCG GCCTGTGGAC ATGGTGCCTA AACCTGGCGC TGCCTCCGTG 
CCCAAAGCCA ATTCTTTAAG TAACCTGGAT CAAGAGAAGA CCTTTAGAAC TCCAGAGCCC 
CAGCAGCCAC AGCCTAAAGT AGATGATATC ATTCGTTCAA ATAACAATGA CCCTGATGAG 
GATGAAGAAT ATTACAGGAA ACAACTGTCC TACTTCGACC GCAGGAGCTT CGACAAACCC 
TCCACACAGC TGACACCTGC CATCAAACCA GCCCAACCCC AAACTCAACC AGCACACTAT 
CCCAGAGCAG AGTCAGTGGA GAAAATCAAC CCAGCTGCTC CATCAAACCC AGCTGTCCCA 
CCACCAACCC TGCCTAAACC TGCAGCAACC CCACCTCTCG ACCCTCACGG ATCTCCCAAA 
GTAAAGCCTC CTAGTCATGA AGACACAGTC CAGAGCACTT ACCTGCCTCA CAAAAGCTTC 
CCAGAGAAAT CCCCCGTCAA CGGCACCGGC GAACCTCCAA AGACCAACAG CACTGCCACG 
TCCAGCTACA ACCGTTTTGT GCCCAAGCCG TACACCACCT CCGCAAGACC CTTCGAGCGC 
AAGTTTGACA GTCCCAAGTT CAATCACAAT CTCCTCCCTA ATGACTCCCA AAACAAGACG 
GATAATGTGA AAGCTCCCAT CACCGCTAAA CCACAGAGCT CACCCCAGCC GGCAGACCAC 
GACAGCGGCC TGGACACCTT CACACGCACC ATGGACCACA AACCCAAATA CCAGCAGAAC 
AACATCAACG CCGTACCCAA GGCAATACCT GTCAGCCCGA GTGCTTTGGA GGATGATGAC 
GATGATGACA GCCACACGGT GGTGGCGACA GCGCGGGGTA TTTTCAACTC TAATGGTGGG 
GTCCTGAGCT CCATTGAGAC AGGAGTAAGC ATTATTATCC CACAGGGAGC CATCCCTGAT 
GGCGTGGAGC AAGAGATCTA CTTTAAAGTG TGTCGGGATA ACAGCATCCT GCCACCTCTG 
GACAAAGAGA AAGGAGAAAC TCTGTTGAGT CCTCTGGTGA TGTGTGGTCC TCACGGTCTG 
AAATTCCTGA AGCCAGTGGA GCTCCGCTTA CCTCACTGTG CGTCTATGAC CCCTGATGGT 
TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ATCCGAAGAG TTGGCAGAAC 
AAATCTCTTC CCGGAGATCC CAACTACCTG GTTGGAGCCA ACTGTGTGTC TGTGCTTATT 
GACCATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303256.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301531.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X10, mRNA.

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CloneID	ODa40331
Clone ID Related Accession (Same CDS sequence)	XM_009303256.3 , XM_009303256.2
Accession Version	XM_009303256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCA AGGCTGCAAT AAATAAGAGT ACAACAATGG AGGAAACTGT CATATGGGAA 
CAGCATACGG TGACTCTTCA CAGGGCACCG GGTTTTGGGT TTGGAATCGC CATCTCAGGG 
GGCCGCGACA ACCCTCATTT TCAGAGCGGA GAGACCTCCA TAGTGATCTC AGATGTACTC 
AAAGGGGGAC CAGCAGAGGG ACTTCTACAA GAGAACGATA GAGTGGTGAT GGTGAATGGC 
GTCTCTATGG ACAACGTGGA GCATGCCTAC GCTGTTCAGC AGCTGAGGAA AAGTGGAAAG 
AATGCCAAAA TTACTATCCG CAGGAAGCGG AAGGTGCAGA TTCCTGTAAG TCGAGGGGGA 
GAAAGAGAAA CTATGTCGGA GCGCGAAGAG GAAGATGACA CAGAGGAGGA TGATTATGAG 
GGTCCAGCTG CTCGTGCTGG GGGAGCGAGT GGAGGCACAG CAAGGAGAAA TGAGCACAGC 
GGCAGCACCG GCCGGCGGGA CCACAGTAAT TCCAGAGAGA GGAGCGTTTC CCCACGCTCA 
GACAGACGAT CAGTTGCGTC CAACAACCCA CAGCGACCTT CCAAAGTCAC ACTCGTCAAG 
TCCCGCAAAA ATGAAGAATA TGGTCTGCGT CTGGCCAGCC ACATCTTTGT TAAGGACATT 
TCTCCTGAGA GTCTTGCAGC GCGTGACGGA AACATACAAG AGGGAGACGT TGTCCTGAAG 
ATCAATGGCA CGGTGACTGA GAATCTGTCC TTGATAGATG CAAAGAAGCT TATAGAGCGA 
TCGAAAGGCA AGTTGAAAAT GGTGGTGCAG AGAGATGAAC GAGCGACACT CCTTAACATT 
CCAGATATGG ATGATAGCAT TCCCTCAGCC AATTCAGACA GAGATGACAT TTCAGAAATC 
CATTCACTGG CATCAGATCA TTCCCATGAG CGCACCCGTC GCAGCCGATC CCGTTCCCCT 
GACAAGCGCT CCGAGCCCTC CGACATCTCT GGTCACTCTC CACAACAGAT GAGCAACGGC 
AGGCTGACGG GCTCTTTTCC CACCAGAAAG CTCCCGCTCC ATAGAAGTCG AGATGAGGAG 
CGCATCTCTA AACCAGCGGC TCTATCCACT CCAGTTAAGA TGGCTGAGGA TGCTCTTCTG 
GCTCCGCCCA GCGAACAGCC CGGGGACAAA CAGATCCCTC CACTGCCAGA GCCGAAACCA 
GTGTATGCAC AACCTGGGCA ACCCGATGTA GATCTGCCAG TGAGCCCGTC TGATGCCCCT 
GTCCCCAGCG TCACCCATGA TGACAGCATC CTCCGCAGGC CCAGTATGAA GCTGGTGAAG 
TTTAAGAAAG GGGAAAGTGT CGGATTGAGG CTGGCTGGAG GAAACGATGT GGGGATCTTT 
GTGGCTGGAG TTCTGGAGGA CAGTCCTGCT GCTAAGGAAG GCCTGGAGGA GGGAGACCAG 
ATACTCAGGG TTAACAATGT TGACTTTGCA AACATTATTC GTGAGGAAGC AGTTCTGTTC 
CTGCTAGATC TGCCCAGAGG TGAGGAGGTC ACCATCCTTG CGCAGAAGAA GAAAGATGTG 
TACCGGCGAA TAGTGGAGTC TGATGTGGGC GACTCCTTCT ACATCAGGAC ACACTTTGAG 
TACGAGAAAG AGTCTCCTTA TGGGCTGAGC TTTAATAAAG GAGAGGTGTT CAGAGTGGTG 
GACACTCTCT ACAATGGCAA ACTGGGCTCC TGGCTGGCTA TCCGCATCGG GAAGAACCAC 
CAGGAGGTGG AAAGAGGCAT CATTCCCAAC AAGAACAGGG CGGAACAGTT ATCAAGCGTA 
CAGTACACAC TCCCCAAGAC TGCAGGTGGT GATCGTGCTG ATTTCTGGAG GTTCAGAGGC 
CTGCGCAGTT CAAAGAGGAA CTTGCGCAAG AGTAGAGAAG ATCTGTCAGC TCAGCCCGTT 
CAGACCAAGT TCCCTGCTTA TGAAAGGGTG GTTCTTAGAG AGGCTGGTTT CCTGAGACCT 
ATTGTGATCT TTGGACCAAT CGCAGATGTG GCACGAGACA AACTGGCAAG AGAGGTGCCT 
GACCTATTCG AGCTCGCAAA GACACAGCTC CCAGATGGAA GGGAAAAGAG TGAACCTAGA 
GATGCCGGCA CTGACCAGCG CAGCTCGGGG ATCATACGCC TTCACACTAT CAAACAGATA 
ATCGACCGGG ATAAGCATGC AGTGCTGGAC ATTACTCCTA ACGCAGTGGA TCGGCTGAAC 
TATGCTCAGT GGTATCCCAT CGTAGTGTTC TTAAATCCTG ACAGCAAGCA GGGTGTGAAG 
AACATGAGGA CCAGACTGTG TTCCGAGTCC AGGAAAAGTG CCAGAAAACT CCACGAGCGC 
GCACTCAAAC TGAGGAAGAA CAACCATCAT CTCTTCACAG CCACTATTAA CATGAATAAC 
ATGAATGATG GCTGGTATGG AGCACTGAAG GACACCATTC AGCAGCAGCA GAACCAGCTG 
GTGTGGGTCT CAGAGGGCAA GGCGGACGGT TCACATGATG ATGACCTGGA CCTTCACGAT 
GACCGTCTGT CCTATTTATC GGCCCCAGGC AGTGAGTACA GTATGTACAG CACGGACAGC 
CGCCACACCT CAGATTACGA GGACACGGAC ACCGAGGGTG GCGCCTACAC TGACCAGGAA 
CTGGACGAAA CCCTGAACGA TGAGGTCGGA CCACCCAGCG AACCAGCCAT CACTCGCTCC 
TCAGAACCTG TCCGCGAAGA CCCGCCCGTC ATCCAGGAGC CCCCCGGATA TGGAGGATAT 
CAGCCCGAAC CCATCAACCG CATCGACCCC GCGGGGTTCA AGGCCCCTGC ACCTCAGCAG 
AAAGCAGAGG CTGCTCTCCC CTCGCAGCCT GAGCCACTGG CAGAGACAGC GCCCCCTGCT 
GTTCATGTAA ATGTAGGGGC CCCGAGTGCC AAGGATCTCA CTCAGGGGGA AATCAGCCCC 
AAGGCTGGCT CTCTCTTCCC ACCTGCCCCT GAGCTTATCC AACCCCCGTC TCCTGACCCC 
AACCAATCCG GACAGCCCGG GTCAGAGTCC AAGATGTACA AGAAGGATAT CTACCGACCC 
AACGAACCGG TGCAGATAAG CCAAAACCCT ATGCAGCCCA ACTACTACCA GGAAGACAAG 
CCATACAGAG ATTATGACCA CCAGCCCTAC CGCTATGATG GAGGCTACAT GGAACCAAAG 
GCTCGTAACT TTGACCCACA TTTTGAGGAC AGCGTGCCTC CATATAATGA TCAATGGGGT 
CAGTATGACC ACACCTCTGG GTATGAACCT CAAACGCTCT ACGAGGATGG TCTGAACCGC 
TTGTATGGTC ACCCTCAGTC ACGAAACGAC ACCGGCTACG ACACAGGTCG ACCTCGATAT 
GGCAAACCAA CCTCTGGACC AATCCGACAC GATGAACCAC CACCATCACG CCCACAATAT 
GACACTCAAC AAACCTCTCG TTCCCCAGAA CCGCCCAAAC AACAATACTA CGACCCTTCC 
CAAAGGCCTT CCTACACTCA AGCTCCACAA AAAGGCTATA TGAACTCTGA AGCACCGGTA 
ACGCCACCCA AACCTGAGTC GCTCTCACCG CCTTTGGAGC CTGCTCTTCC TCCTCCACCT 
CCTCCAGCCG AAGCAGAGGA TGATCCCGCC ATGAAGCCCC AGTCGGTGCT CACCAGGGTG 
AAGATGTTCG AGAACAAGCG ATCGGTGTCT GTAGACAGAG CCAGAGAGTC TGCAGACTCT 
GGTATTCGGC CTGTGGACAT GGTGCCTAAA CCTGGCGCTG CCTCCGTGCC CAAAGCCAAT 
TCTTTAAGTA ACCTGGATCA AGAGAAGACC TTTAGAACTC CAGAGCCCCA GCAGCCACAG 
CCTAAAGTAG ATGATATCAT TCGTTCAAAT AACAATGACC CTGATGAGGA TGAAGAATAT 
TACAGGAAAC AACTGTCCTA CTTCGACCGC AGGAGCTTCG ACAAACCCTC CACACAGCTG 
ACACCTGCCA TCAAACCAGC CCAACCCCAA ACTCAACCAG CACACTATCC CAGAGCAGAG 
TCAGTGGAGA AAATCAACCC AGCTGCTCCA TCAAACCCAG CTGTCCCACC ACCAACCCTG 
CCTAAACCTG CAGCAACCCC ACCTCTCGAC CCTCACGGAT CTCCCAAAGT AAAGCCTCCT 
AGTCATGAAG ACACAGTCCA GAGCACTTAC CTGCCTCACA AAAGCTTCCC AGAGAAATCC 
CCCGTCAACG GCACCGGCGA ACCTCCAAAG ACCAACAGCA CTGCCACGTC CAGCTACAAC 
CGTTTTGTGC CCAAGCCGTA CACCACCTCC GCAAGACCCT TCGAGCGCAA GTTTGACAGT 
CCCAAGTTCA ATCACAATCT CCTCCCTAAT GACTCCCAAA ACAAGACGGA TAATGTGAAA 
GCTCCCATCA CCGCTAAACC ACAGAGCTCA CCCCAGCCGG CAGACCACGA CAGCGGCCTG 
GACACCTTCA CACGCACCAT GGACCACAAA CCCAAATACC AGCAGAACAA CATCAACGCC 
GTACCCAAGG CAATACCTGT CAGCCCGAGT GCTTTGGAGG ATGATGACGA TGATGACAGC 
CACACGGTGG TGGCGACAGC GCGGGGTATT TTCAACTCTA ATGGTGGGGT CCTGAGCTCC 
ATTGAGACAG GAGTAAGCAT TATTATCCCA CAGGGAGCCA TCCCTGATGG CGTGGAGCAA 
GAGATCTACT TTAAAGTGTG TCGGGATAAC AGCATCCTGC CACCTCTGGA CAAAGAGAAA 
GGAGAAACTC TGTTGAGTCC TCTGGTGATG TGTGGTCCTC ACGGTCTGAA ATTCCTGAAG 
CCAGTGGAGC TCCGCTTACC TCACTGTGCG TCTATGACCC CTGATGGTTG GTCTTTTGCT 
CTAAAATCCT CCGACTCCTC GTCGGGTGAT CCGAAGAGTT GGCAGAACAA ATCTCTTCCC 
GGAGATCCCA ACTACCTGGT TGGAGCCAAC TGTGTGTCTG TGCTTATTGA CCATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301531.1
CDS	410..5389
Translation	MSAKAAINKSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303256.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303256.3

ORF Insert Sequence:

ATGTCTGCCA AGGCTGCAAT AAATAAGAGT ACAACAATGG AGGAAACTGT CATATGGGAA 
CAGCATACGG TGACTCTTCA CAGGGCACCG GGTTTTGGGT TTGGAATCGC CATCTCAGGG 
GGCCGCGACA ACCCTCATTT TCAGAGCGGA GAGACCTCCA TAGTGATCTC AGATGTACTC 
AAAGGGGGAC CAGCAGAGGG ACTTCTACAA GAGAACGATA GAGTGGTGAT GGTGAATGGC 
GTCTCTATGG ACAACGTGGA GCATGCCTAC GCTGTTCAGC AGCTGAGGAA AAGTGGAAAG 
AATGCCAAAA TTACTATCCG CAGGAAGCGG AAGGTGCAGA TTCCTGTAAG TCGAGGGGGA 
GAAAGAGAAA CTATGTCGGA GCGCGAAGAG GAAGATGACA CAGAGGAGGA TGATTATGAG 
GGTCCAGCTG CTCGTGCTGG GGGAGCGAGT GGAGGCACAG CAAGGAGAAA TGAGCACAGC 
GGCAGCACCG GCCGGCGGGA CCACAGTAAT TCCAGAGAGA GGAGCGTTTC CCCACGCTCA 
GACAGACGAT CAGTTGCGTC CAACAACCCA CAGCGACCTT CCAAAGTCAC ACTCGTCAAG 
TCCCGCAAAA ATGAAGAATA TGGTCTGCGT CTGGCCAGCC ACATCTTTGT TAAGGACATT 
TCTCCTGAGA GTCTTGCAGC GCGTGACGGA AACATACAAG AGGGAGACGT TGTCCTGAAG 
ATCAATGGCA CGGTGACTGA GAATCTGTCC TTGATAGATG CAAAGAAGCT TATAGAGCGA 
TCGAAAGGCA AGTTGAAAAT GGTGGTGCAG AGAGATGAAC GAGCGACACT CCTTAACATT 
CCAGATATGG ATGATAGCAT TCCCTCAGCC AATTCAGACA GAGATGACAT TTCAGAAATC 
CATTCACTGG CATCAGATCA TTCCCATGAG CGCACCCGTC GCAGCCGATC CCGTTCCCCT 
GACAAGCGCT CCGAGCCCTC CGACATCTCT GGTCACTCTC CACAACAGAT GAGCAACGGC 
AGGCTGACGG GCTCTTTTCC CACCAGAAAG CTCCCGCTCC ATAGAAGTCG AGATGAGGAG 
CGCATCTCTA AACCAGCGGC TCTATCCACT CCAGTTAAGA TGGCTGAGGA TGCTCTTCTG 
GCTCCGCCCA GCGAACAGCC CGGGGACAAA CAGATCCCTC CACTGCCAGA GCCGAAACCA 
GTGTATGCAC AACCTGGGCA ACCCGATGTA GATCTGCCAG TGAGCCCGTC TGATGCCCCT 
GTCCCCAGCG TCACCCATGA TGACAGCATC CTCCGCAGGC CCAGTATGAA GCTGGTGAAG 
TTTAAGAAAG GGGAAAGTGT CGGATTGAGG CTGGCTGGAG GAAACGATGT GGGGATCTTT 
GTGGCTGGAG TTCTGGAGGA CAGTCCTGCT GCTAAGGAAG GCCTGGAGGA GGGAGACCAG 
ATACTCAGGG TTAACAATGT TGACTTTGCA AACATTATTC GTGAGGAAGC AGTTCTGTTC 
CTGCTAGATC TGCCCAGAGG TGAGGAGGTC ACCATCCTTG CGCAGAAGAA GAAAGATGTG 
TACCGGCGAA TAGTGGAGTC TGATGTGGGC GACTCCTTCT ACATCAGGAC ACACTTTGAG 
TACGAGAAAG AGTCTCCTTA TGGGCTGAGC TTTAATAAAG GAGAGGTGTT CAGAGTGGTG 
GACACTCTCT ACAATGGCAA ACTGGGCTCC TGGCTGGCTA TCCGCATCGG GAAGAACCAC 
CAGGAGGTGG AAAGAGGCAT CATTCCCAAC AAGAACAGGG CGGAACAGTT ATCAAGCGTA 
CAGTACACAC TCCCCAAGAC TGCAGGTGGT GATCGTGCTG ATTTCTGGAG GTTCAGAGGC 
CTGCGCAGTT CAAAGAGGAA CTTGCGCAAG AGTAGAGAAG ATCTGTCAGC TCAGCCCGTT 
CAGACCAAGT TCCCTGCTTA TGAAAGGGTG GTTCTTAGAG AGGCTGGTTT CCTGAGACCT 
ATTGTGATCT TTGGACCAAT CGCAGATGTG GCACGAGACA AACTGGCAAG AGAGGTGCCT 
GACCTATTCG AGCTCGCAAA GACACAGCTC CCAGATGGAA GGGAAAAGAG TGAACCTAGA 
GATGCCGGCA CTGACCAGCG CAGCTCGGGG ATCATACGCC TTCACACTAT CAAACAGATA 
ATCGACCGGG ATAAGCATGC AGTGCTGGAC ATTACTCCTA ACGCAGTGGA TCGGCTGAAC 
TATGCTCAGT GGTATCCCAT CGTAGTGTTC TTAAATCCTG ACAGCAAGCA GGGTGTGAAG 
AACATGAGGA CCAGACTGTG TTCCGAGTCC AGGAAAAGTG CCAGAAAACT CCACGAGCGC 
GCACTCAAAC TGAGGAAGAA CAACCATCAT CTCTTCACAG CCACTATTAA CATGAATAAC 
ATGAATGATG GCTGGTATGG AGCACTGAAG GACACCATTC AGCAGCAGCA GAACCAGCTG 
GTGTGGGTCT CAGAGGGCAA GGCGGACGGT TCACATGATG ATGACCTGGA CCTTCACGAT 
GACCGTCTGT CCTATTTATC GGCCCCAGGC AGTGAGTACA GTATGTACAG CACGGACAGC 
CGCCACACCT CAGATTACGA GGACACGGAC ACCGAGGGTG GCGCCTACAC TGACCAGGAA 
CTGGACGAAA CCCTGAACGA TGAGGTCGGA CCACCCAGCG AACCAGCCAT CACTCGCTCC 
TCAGAACCTG TCCGCGAAGA CCCGCCCGTC ATCCAGGAGC CCCCCGGATA TGGAGGATAT 
CAGCCCGAAC CCATCAACCG CATCGACCCC GCGGGGTTCA AGGCCCCTGC ACCTCAGCAG 
AAAGCAGAGG CTGCTCTCCC CTCGCAGCCT GAGCCACTGG CAGAGACAGC GCCCCCTGCT 
GTTCATGTAA ATGTAGGGGC CCCGAGTGCC AAGGATCTCA CTCAGGGGGA AATCAGCCCC 
AAGGCTGGCT CTCTCTTCCC ACCTGCCCCT GAGCTTATCC AACCCCCGTC TCCTGACCCC 
AACCAATCCG GACAGCCCGG GTCAGAGTCC AAGATGTACA AGAAGGATAT CTACCGACCC 
AACGAACCGG TGCAGATAAG CCAAAACCCT ATGCAGCCCA ACTACTACCA GGAAGACAAG 
CCATACAGAG ATTATGACCA CCAGCCCTAC CGCTATGATG GAGGCTACAT GGAACCAAAG 
GCTCGTAACT TTGACCCACA TTTTGAGGAC AGCGTGCCTC CATATAATGA TCAATGGGGT 
CAGTATGACC ACACCTCTGG GTATGAACCT CAAACGCTCT ACGAGGATGG TCTGAACCGC 
TTGTATGGTC ACCCTCAGTC ACGAAACGAC ACCGGCTACG ACACAGGTCG ACCTCGATAT 
GGCAAACCAA CCTCTGGACC AATCCGACAC GATGAACCAC CACCATCACG CCCACAATAT 
GACACTCAAC AAACCTCTCG TTCCCCAGAA CCGCCCAAAC AACAATACTA CGACCCTTCC 
CAAAGGCCTT CCTACACTCA AGCTCCACAA AAAGGCTATA TGAACTCTGA AGCACCGGTA 
ACGCCACCCA AACCTGAGTC GCTCTCACCG CCTTTGGAGC CTGCTCTTCC TCCTCCACCT 
CCTCCAGCCG AAGCAGAGGA TGATCCCGCC ATGAAGCCCC AGTCGGTGCT CACCAGGGTG 
AAGATGTTCG AGAACAAGCG ATCGGTGTCT GTAGACAGAG CCAGAGAGTC TGCAGACTCT 
GGTATTCGGC CTGTGGACAT GGTGCCTAAA CCTGGCGCTG CCTCCGTGCC CAAAGCCAAT 
TCTTTAAGTA ACCTGGATCA AGAGAAGACC TTTAGAACTC CAGAGCCCCA GCAGCCACAG 
CCTAAAGTAG ATGATATCAT TCGTTCAAAT AACAATGACC CTGATGAGGA TGAAGAATAT 
TACAGGAAAC AACTGTCCTA CTTCGACCGC AGGAGCTTCG ACAAACCCTC CACACAGCTG 
ACACCTGCCA TCAAACCAGC CCAACCCCAA ACTCAACCAG CACACTATCC CAGAGCAGAG 
TCAGTGGAGA AAATCAACCC AGCTGCTCCA TCAAACCCAG CTGTCCCACC ACCAACCCTG 
CCTAAACCTG CAGCAACCCC ACCTCTCGAC CCTCACGGAT CTCCCAAAGT AAAGCCTCCT 
AGTCATGAAG ACACAGTCCA GAGCACTTAC CTGCCTCACA AAAGCTTCCC AGAGAAATCC 
CCCGTCAACG GCACCGGCGA ACCTCCAAAG ACCAACAGCA CTGCCACGTC CAGCTACAAC 
CGTTTTGTGC CCAAGCCGTA CACCACCTCC GCAAGACCCT TCGAGCGCAA GTTTGACAGT 
CCCAAGTTCA ATCACAATCT CCTCCCTAAT GACTCCCAAA ACAAGACGGA TAATGTGAAA 
GCTCCCATCA CCGCTAAACC ACAGAGCTCA CCCCAGCCGG CAGACCACGA CAGCGGCCTG 
GACACCTTCA CACGCACCAT GGACCACAAA CCCAAATACC AGCAGAACAA CATCAACGCC 
GTACCCAAGG CAATACCTGT CAGCCCGAGT GCTTTGGAGG ATGATGACGA TGATGACAGC 
CACACGGTGG TGGCGACAGC GCGGGGTATT TTCAACTCTA ATGGTGGGGT CCTGAGCTCC 
ATTGAGACAG GAGTAAGCAT TATTATCCCA CAGGGAGCCA TCCCTGATGG CGTGGAGCAA 
GAGATCTACT TTAAAGTGTG TCGGGATAAC AGCATCCTGC CACCTCTGGA CAAAGAGAAA 
GGAGAAACTC TGTTGAGTCC TCTGGTGATG TGTGGTCCTC ACGGTCTGAA ATTCCTGAAG 
CCAGTGGAGC TCCGCTTACC TCACTGTGCG TCTATGACCC CTGATGGTTG GTCTTTTGCT 
CTAAAATCCT CCGACTCCTC GTCGGGTGAT CCGAAGAGTT GGCAGAACAA ATCTCTTCCC 
GGAGATCCCA ACTACCTGGT TGGAGCCAAC TGTGTGTCTG TGCTTATTGA CCATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303256.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301531.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X9, mRNA.

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CloneID	ODa40331
Clone ID Related Accession (Same CDS sequence)	XM_009303256.3 , XM_009303256.2
Accession Version	XM_009303256.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCA AGGCTGCAAT AAATAAGAGT ACAACAATGG AGGAAACTGT CATATGGGAA 
CAGCATACGG TGACTCTTCA CAGGGCACCG GGTTTTGGGT TTGGAATCGC CATCTCAGGG 
GGCCGCGACA ACCCTCATTT TCAGAGCGGA GAGACCTCCA TAGTGATCTC AGATGTACTC 
AAAGGGGGAC CAGCAGAGGG ACTTCTACAA GAGAACGATA GAGTGGTGAT GGTGAATGGC 
GTCTCTATGG ACAACGTGGA GCATGCCTAC GCTGTTCAGC AGCTGAGGAA AAGTGGAAAG 
AATGCCAAAA TTACTATCCG CAGGAAGCGG AAGGTGCAGA TTCCTGTAAG TCGAGGGGGA 
GAAAGAGAAA CTATGTCGGA GCGCGAAGAG GAAGATGACA CAGAGGAGGA TGATTATGAG 
GGTCCAGCTG CTCGTGCTGG GGGAGCGAGT GGAGGCACAG CAAGGAGAAA TGAGCACAGC 
GGCAGCACCG GCCGGCGGGA CCACAGTAAT TCCAGAGAGA GGAGCGTTTC CCCACGCTCA 
GACAGACGAT CAGTTGCGTC CAACAACCCA CAGCGACCTT CCAAAGTCAC ACTCGTCAAG 
TCCCGCAAAA ATGAAGAATA TGGTCTGCGT CTGGCCAGCC ACATCTTTGT TAAGGACATT 
TCTCCTGAGA GTCTTGCAGC GCGTGACGGA AACATACAAG AGGGAGACGT TGTCCTGAAG 
ATCAATGGCA CGGTGACTGA GAATCTGTCC TTGATAGATG CAAAGAAGCT TATAGAGCGA 
TCGAAAGGCA AGTTGAAAAT GGTGGTGCAG AGAGATGAAC GAGCGACACT CCTTAACATT 
CCAGATATGG ATGATAGCAT TCCCTCAGCC AATTCAGACA GAGATGACAT TTCAGAAATC 
CATTCACTGG CATCAGATCA TTCCCATGAG CGCACCCGTC GCAGCCGATC CCGTTCCCCT 
GACAAGCGCT CCGAGCCCTC CGACATCTCT GGTCACTCTC CACAACAGAT GAGCAACGGC 
AGGCTGACGG GCTCTTTTCC CACCAGAAAG CTCCCGCTCC ATAGAAGTCG AGATGAGGAG 
CGCATCTCTA AACCAGCGGC TCTATCCACT CCAGTTAAGA TGGCTGAGGA TGCTCTTCTG 
GCTCCGCCCA GCGAACAGCC CGGGGACAAA CAGATCCCTC CACTGCCAGA GCCGAAACCA 
GTGTATGCAC AACCTGGGCA ACCCGATGTA GATCTGCCAG TGAGCCCGTC TGATGCCCCT 
GTCCCCAGCG TCACCCATGA TGACAGCATC CTCCGCAGGC CCAGTATGAA GCTGGTGAAG 
TTTAAGAAAG GGGAAAGTGT CGGATTGAGG CTGGCTGGAG GAAACGATGT GGGGATCTTT 
GTGGCTGGAG TTCTGGAGGA CAGTCCTGCT GCTAAGGAAG GCCTGGAGGA GGGAGACCAG 
ATACTCAGGG TTAACAATGT TGACTTTGCA AACATTATTC GTGAGGAAGC AGTTCTGTTC 
CTGCTAGATC TGCCCAGAGG TGAGGAGGTC ACCATCCTTG CGCAGAAGAA GAAAGATGTG 
TACCGGCGAA TAGTGGAGTC TGATGTGGGC GACTCCTTCT ACATCAGGAC ACACTTTGAG 
TACGAGAAAG AGTCTCCTTA TGGGCTGAGC TTTAATAAAG GAGAGGTGTT CAGAGTGGTG 
GACACTCTCT ACAATGGCAA ACTGGGCTCC TGGCTGGCTA TCCGCATCGG GAAGAACCAC 
CAGGAGGTGG AAAGAGGCAT CATTCCCAAC AAGAACAGGG CGGAACAGTT ATCAAGCGTA 
CAGTACACAC TCCCCAAGAC TGCAGGTGGT GATCGTGCTG ATTTCTGGAG GTTCAGAGGC 
CTGCGCAGTT CAAAGAGGAA CTTGCGCAAG AGTAGAGAAG ATCTGTCAGC TCAGCCCGTT 
CAGACCAAGT TCCCTGCTTA TGAAAGGGTG GTTCTTAGAG AGGCTGGTTT CCTGAGACCT 
ATTGTGATCT TTGGACCAAT CGCAGATGTG GCACGAGACA AACTGGCAAG AGAGGTGCCT 
GACCTATTCG AGCTCGCAAA GACACAGCTC CCAGATGGAA GGGAAAAGAG TGAACCTAGA 
GATGCCGGCA CTGACCAGCG CAGCTCGGGG ATCATACGCC TTCACACTAT CAAACAGATA 
ATCGACCGGG ATAAGCATGC AGTGCTGGAC ATTACTCCTA ACGCAGTGGA TCGGCTGAAC 
TATGCTCAGT GGTATCCCAT CGTAGTGTTC TTAAATCCTG ACAGCAAGCA GGGTGTGAAG 
AACATGAGGA CCAGACTGTG TTCCGAGTCC AGGAAAAGTG CCAGAAAACT CCACGAGCGC 
GCACTCAAAC TGAGGAAGAA CAACCATCAT CTCTTCACAG CCACTATTAA CATGAATAAC 
ATGAATGATG GCTGGTATGG AGCACTGAAG GACACCATTC AGCAGCAGCA GAACCAGCTG 
GTGTGGGTCT CAGAGGGCAA GGCGGACGGT TCACATGATG ATGACCTGGA CCTTCACGAT 
GACCGTCTGT CCTATTTATC GGCCCCAGGC AGTGAGTACA GTATGTACAG CACGGACAGC 
CGCCACACCT CAGATTACGA GGACACGGAC ACCGAGGGTG GCGCCTACAC TGACCAGGAA 
CTGGACGAAA CCCTGAACGA TGAGGTCGGA CCACCCAGCG AACCAGCCAT CACTCGCTCC 
TCAGAACCTG TCCGCGAAGA CCCGCCCGTC ATCCAGGAGC CCCCCGGATA TGGAGGATAT 
CAGCCCGAAC CCATCAACCG CATCGACCCC GCGGGGTTCA AGGCCCCTGC ACCTCAGCAG 
AAAGCAGAGG CTGCTCTCCC CTCGCAGCCT GAGCCACTGG CAGAGACAGC GCCCCCTGCT 
GTTCATGTAA ATGTAGGGGC CCCGAGTGCC AAGGATCTCA CTCAGGGGGA AATCAGCCCC 
AAGGCTGGCT CTCTCTTCCC ACCTGCCCCT GAGCTTATCC AACCCCCGTC TCCTGACCCC 
AACCAATCCG GACAGCCCGG GTCAGAGTCC AAGATGTACA AGAAGGATAT CTACCGACCC 
AACGAACCGG TGCAGATAAG CCAAAACCCT ATGCAGCCCA ACTACTACCA GGAAGACAAG 
CCATACAGAG ATTATGACCA CCAGCCCTAC CGCTATGATG GAGGCTACAT GGAACCAAAG 
GCTCGTAACT TTGACCCACA TTTTGAGGAC AGCGTGCCTC CATATAATGA TCAATGGGGT 
CAGTATGACC ACACCTCTGG GTATGAACCT CAAACGCTCT ACGAGGATGG TCTGAACCGC 
TTGTATGGTC ACCCTCAGTC ACGAAACGAC ACCGGCTACG ACACAGGTCG ACCTCGATAT 
GGCAAACCAA CCTCTGGACC AATCCGACAC GATGAACCAC CACCATCACG CCCACAATAT 
GACACTCAAC AAACCTCTCG TTCCCCAGAA CCGCCCAAAC AACAATACTA CGACCCTTCC 
CAAAGGCCTT CCTACACTCA AGCTCCACAA AAAGGCTATA TGAACTCTGA AGCACCGGTA 
ACGCCACCCA AACCTGAGTC GCTCTCACCG CCTTTGGAGC CTGCTCTTCC TCCTCCACCT 
CCTCCAGCCG AAGCAGAGGA TGATCCCGCC ATGAAGCCCC AGTCGGTGCT CACCAGGGTG 
AAGATGTTCG AGAACAAGCG ATCGGTGTCT GTAGACAGAG CCAGAGAGTC TGCAGACTCT 
GGTATTCGGC CTGTGGACAT GGTGCCTAAA CCTGGCGCTG CCTCCGTGCC CAAAGCCAAT 
TCTTTAAGTA ACCTGGATCA AGAGAAGACC TTTAGAACTC CAGAGCCCCA GCAGCCACAG 
CCTAAAGTAG ATGATATCAT TCGTTCAAAT AACAATGACC CTGATGAGGA TGAAGAATAT 
TACAGGAAAC AACTGTCCTA CTTCGACCGC AGGAGCTTCG ACAAACCCTC CACACAGCTG 
ACACCTGCCA TCAAACCAGC CCAACCCCAA ACTCAACCAG CACACTATCC CAGAGCAGAG 
TCAGTGGAGA AAATCAACCC AGCTGCTCCA TCAAACCCAG CTGTCCCACC ACCAACCCTG 
CCTAAACCTG CAGCAACCCC ACCTCTCGAC CCTCACGGAT CTCCCAAAGT AAAGCCTCCT 
AGTCATGAAG ACACAGTCCA GAGCACTTAC CTGCCTCACA AAAGCTTCCC AGAGAAATCC 
CCCGTCAACG GCACCGGCGA ACCTCCAAAG ACCAACAGCA CTGCCACGTC CAGCTACAAC 
CGTTTTGTGC CCAAGCCGTA CACCACCTCC GCAAGACCCT TCGAGCGCAA GTTTGACAGT 
CCCAAGTTCA ATCACAATCT CCTCCCTAAT GACTCCCAAA ACAAGACGGA TAATGTGAAA 
GCTCCCATCA CCGCTAAACC ACAGAGCTCA CCCCAGCCGG CAGACCACGA CAGCGGCCTG 
GACACCTTCA CACGCACCAT GGACCACAAA CCCAAATACC AGCAGAACAA CATCAACGCC 
GTACCCAAGG CAATACCTGT CAGCCCGAGT GCTTTGGAGG ATGATGACGA TGATGACAGC 
CACACGGTGG TGGCGACAGC GCGGGGTATT TTCAACTCTA ATGGTGGGGT CCTGAGCTCC 
ATTGAGACAG GAGTAAGCAT TATTATCCCA CAGGGAGCCA TCCCTGATGG CGTGGAGCAA 
GAGATCTACT TTAAAGTGTG TCGGGATAAC AGCATCCTGC CACCTCTGGA CAAAGAGAAA 
GGAGAAACTC TGTTGAGTCC TCTGGTGATG TGTGGTCCTC ACGGTCTGAA ATTCCTGAAG 
CCAGTGGAGC TCCGCTTACC TCACTGTGCG TCTATGACCC CTGATGGTTG GTCTTTTGCT 
CTAAAATCCT CCGACTCCTC GTCGGGTGAT CCGAAGAGTT GGCAGAACAA ATCTCTTCCC 
GGAGATCCCA ACTACCTGGT TGGAGCCAAC TGTGTGTCTG TGCTTATTGA CCATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301531.1
CDS	419..5398
Translation	MSAKAAINKSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303256.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303256.2

ORF Insert Sequence:

ATGTCTGCCA AGGCTGCAAT AAATAAGAGT ACAACAATGG AGGAAACTGT CATATGGGAA 
CAGCATACGG TGACTCTTCA CAGGGCACCG GGTTTTGGGT TTGGAATCGC CATCTCAGGG 
GGCCGCGACA ACCCTCATTT TCAGAGCGGA GAGACCTCCA TAGTGATCTC AGATGTACTC 
AAAGGGGGAC CAGCAGAGGG ACTTCTACAA GAGAACGATA GAGTGGTGAT GGTGAATGGC 
GTCTCTATGG ACAACGTGGA GCATGCCTAC GCTGTTCAGC AGCTGAGGAA AAGTGGAAAG 
AATGCCAAAA TTACTATCCG CAGGAAGCGG AAGGTGCAGA TTCCTGTAAG TCGAGGGGGA 
GAAAGAGAAA CTATGTCGGA GCGCGAAGAG GAAGATGACA CAGAGGAGGA TGATTATGAG 
GGTCCAGCTG CTCGTGCTGG GGGAGCGAGT GGAGGCACAG CAAGGAGAAA TGAGCACAGC 
GGCAGCACCG GCCGGCGGGA CCACAGTAAT TCCAGAGAGA GGAGCGTTTC CCCACGCTCA 
GACAGACGAT CAGTTGCGTC CAACAACCCA CAGCGACCTT CCAAAGTCAC ACTCGTCAAG 
TCCCGCAAAA ATGAAGAATA TGGTCTGCGT CTGGCCAGCC ACATCTTTGT TAAGGACATT 
TCTCCTGAGA GTCTTGCAGC GCGTGACGGA AACATACAAG AGGGAGACGT TGTCCTGAAG 
ATCAATGGCA CGGTGACTGA GAATCTGTCC TTGATAGATG CAAAGAAGCT TATAGAGCGA 
TCGAAAGGCA AGTTGAAAAT GGTGGTGCAG AGAGATGAAC GAGCGACACT CCTTAACATT 
CCAGATATGG ATGATAGCAT TCCCTCAGCC AATTCAGACA GAGATGACAT TTCAGAAATC 
CATTCACTGG CATCAGATCA TTCCCATGAG CGCACCCGTC GCAGCCGATC CCGTTCCCCT 
GACAAGCGCT CCGAGCCCTC CGACATCTCT GGTCACTCTC CACAACAGAT GAGCAACGGC 
AGGCTGACGG GCTCTTTTCC CACCAGAAAG CTCCCGCTCC ATAGAAGTCG AGATGAGGAG 
CGCATCTCTA AACCAGCGGC TCTATCCACT CCAGTTAAGA TGGCTGAGGA TGCTCTTCTG 
GCTCCGCCCA GCGAACAGCC CGGGGACAAA CAGATCCCTC CACTGCCAGA GCCGAAACCA 
GTGTATGCAC AACCTGGGCA ACCCGATGTA GATCTGCCAG TGAGCCCGTC TGATGCCCCT 
GTCCCCAGCG TCACCCATGA TGACAGCATC CTCCGCAGGC CCAGTATGAA GCTGGTGAAG 
TTTAAGAAAG GGGAAAGTGT CGGATTGAGG CTGGCTGGAG GAAACGATGT GGGGATCTTT 
GTGGCTGGAG TTCTGGAGGA CAGTCCTGCT GCTAAGGAAG GCCTGGAGGA GGGAGACCAG 
ATACTCAGGG TTAACAATGT TGACTTTGCA AACATTATTC GTGAGGAAGC AGTTCTGTTC 
CTGCTAGATC TGCCCAGAGG TGAGGAGGTC ACCATCCTTG CGCAGAAGAA GAAAGATGTG 
TACCGGCGAA TAGTGGAGTC TGATGTGGGC GACTCCTTCT ACATCAGGAC ACACTTTGAG 
TACGAGAAAG AGTCTCCTTA TGGGCTGAGC TTTAATAAAG GAGAGGTGTT CAGAGTGGTG 
GACACTCTCT ACAATGGCAA ACTGGGCTCC TGGCTGGCTA TCCGCATCGG GAAGAACCAC 
CAGGAGGTGG AAAGAGGCAT CATTCCCAAC AAGAACAGGG CGGAACAGTT ATCAAGCGTA 
CAGTACACAC TCCCCAAGAC TGCAGGTGGT GATCGTGCTG ATTTCTGGAG GTTCAGAGGC 
CTGCGCAGTT CAAAGAGGAA CTTGCGCAAG AGTAGAGAAG ATCTGTCAGC TCAGCCCGTT 
CAGACCAAGT TCCCTGCTTA TGAAAGGGTG GTTCTTAGAG AGGCTGGTTT CCTGAGACCT 
ATTGTGATCT TTGGACCAAT CGCAGATGTG GCACGAGACA AACTGGCAAG AGAGGTGCCT 
GACCTATTCG AGCTCGCAAA GACACAGCTC CCAGATGGAA GGGAAAAGAG TGAACCTAGA 
GATGCCGGCA CTGACCAGCG CAGCTCGGGG ATCATACGCC TTCACACTAT CAAACAGATA 
ATCGACCGGG ATAAGCATGC AGTGCTGGAC ATTACTCCTA ACGCAGTGGA TCGGCTGAAC 
TATGCTCAGT GGTATCCCAT CGTAGTGTTC TTAAATCCTG ACAGCAAGCA GGGTGTGAAG 
AACATGAGGA CCAGACTGTG TTCCGAGTCC AGGAAAAGTG CCAGAAAACT CCACGAGCGC 
GCACTCAAAC TGAGGAAGAA CAACCATCAT CTCTTCACAG CCACTATTAA CATGAATAAC 
ATGAATGATG GCTGGTATGG AGCACTGAAG GACACCATTC AGCAGCAGCA GAACCAGCTG 
GTGTGGGTCT CAGAGGGCAA GGCGGACGGT TCACATGATG ATGACCTGGA CCTTCACGAT 
GACCGTCTGT CCTATTTATC GGCCCCAGGC AGTGAGTACA GTATGTACAG CACGGACAGC 
CGCCACACCT CAGATTACGA GGACACGGAC ACCGAGGGTG GCGCCTACAC TGACCAGGAA 
CTGGACGAAA CCCTGAACGA TGAGGTCGGA CCACCCAGCG AACCAGCCAT CACTCGCTCC 
TCAGAACCTG TCCGCGAAGA CCCGCCCGTC ATCCAGGAGC CCCCCGGATA TGGAGGATAT 
CAGCCCGAAC CCATCAACCG CATCGACCCC GCGGGGTTCA AGGCCCCTGC ACCTCAGCAG 
AAAGCAGAGG CTGCTCTCCC CTCGCAGCCT GAGCCACTGG CAGAGACAGC GCCCCCTGCT 
GTTCATGTAA ATGTAGGGGC CCCGAGTGCC AAGGATCTCA CTCAGGGGGA AATCAGCCCC 
AAGGCTGGCT CTCTCTTCCC ACCTGCCCCT GAGCTTATCC AACCCCCGTC TCCTGACCCC 
AACCAATCCG GACAGCCCGG GTCAGAGTCC AAGATGTACA AGAAGGATAT CTACCGACCC 
AACGAACCGG TGCAGATAAG CCAAAACCCT ATGCAGCCCA ACTACTACCA GGAAGACAAG 
CCATACAGAG ATTATGACCA CCAGCCCTAC CGCTATGATG GAGGCTACAT GGAACCAAAG 
GCTCGTAACT TTGACCCACA TTTTGAGGAC AGCGTGCCTC CATATAATGA TCAATGGGGT 
CAGTATGACC ACACCTCTGG GTATGAACCT CAAACGCTCT ACGAGGATGG TCTGAACCGC 
TTGTATGGTC ACCCTCAGTC ACGAAACGAC ACCGGCTACG ACACAGGTCG ACCTCGATAT 
GGCAAACCAA CCTCTGGACC AATCCGACAC GATGAACCAC CACCATCACG CCCACAATAT 
GACACTCAAC AAACCTCTCG TTCCCCAGAA CCGCCCAAAC AACAATACTA CGACCCTTCC 
CAAAGGCCTT CCTACACTCA AGCTCCACAA AAAGGCTATA TGAACTCTGA AGCACCGGTA 
ACGCCACCCA AACCTGAGTC GCTCTCACCG CCTTTGGAGC CTGCTCTTCC TCCTCCACCT 
CCTCCAGCCG AAGCAGAGGA TGATCCCGCC ATGAAGCCCC AGTCGGTGCT CACCAGGGTG 
AAGATGTTCG AGAACAAGCG ATCGGTGTCT GTAGACAGAG CCAGAGAGTC TGCAGACTCT 
GGTATTCGGC CTGTGGACAT GGTGCCTAAA CCTGGCGCTG CCTCCGTGCC CAAAGCCAAT 
TCTTTAAGTA ACCTGGATCA AGAGAAGACC TTTAGAACTC CAGAGCCCCA GCAGCCACAG 
CCTAAAGTAG ATGATATCAT TCGTTCAAAT AACAATGACC CTGATGAGGA TGAAGAATAT 
TACAGGAAAC AACTGTCCTA CTTCGACCGC AGGAGCTTCG ACAAACCCTC CACACAGCTG 
ACACCTGCCA TCAAACCAGC CCAACCCCAA ACTCAACCAG CACACTATCC CAGAGCAGAG 
TCAGTGGAGA AAATCAACCC AGCTGCTCCA TCAAACCCAG CTGTCCCACC ACCAACCCTG 
CCTAAACCTG CAGCAACCCC ACCTCTCGAC CCTCACGGAT CTCCCAAAGT AAAGCCTCCT 
AGTCATGAAG ACACAGTCCA GAGCACTTAC CTGCCTCACA AAAGCTTCCC AGAGAAATCC 
CCCGTCAACG GCACCGGCGA ACCTCCAAAG ACCAACAGCA CTGCCACGTC CAGCTACAAC 
CGTTTTGTGC CCAAGCCGTA CACCACCTCC GCAAGACCCT TCGAGCGCAA GTTTGACAGT 
CCCAAGTTCA ATCACAATCT CCTCCCTAAT GACTCCCAAA ACAAGACGGA TAATGTGAAA 
GCTCCCATCA CCGCTAAACC ACAGAGCTCA CCCCAGCCGG CAGACCACGA CAGCGGCCTG 
GACACCTTCA CACGCACCAT GGACCACAAA CCCAAATACC AGCAGAACAA CATCAACGCC 
GTACCCAAGG CAATACCTGT CAGCCCGAGT GCTTTGGAGG ATGATGACGA TGATGACAGC 
CACACGGTGG TGGCGACAGC GCGGGGTATT TTCAACTCTA ATGGTGGGGT CCTGAGCTCC 
ATTGAGACAG GAGTAAGCAT TATTATCCCA CAGGGAGCCA TCCCTGATGG CGTGGAGCAA 
GAGATCTACT TTAAAGTGTG TCGGGATAAC AGCATCCTGC CACCTCTGGA CAAAGAGAAA 
GGAGAAACTC TGTTGAGTCC TCTGGTGATG TGTGGTCCTC ACGGTCTGAA ATTCCTGAAG 
CCAGTGGAGC TCCGCTTACC TCACTGTGCG TCTATGACCC CTGATGGTTG GTCTTTTGCT 
CTAAAATCCT CCGACTCCTC GTCGGGTGAT CCGAAGAGTT GGCAGAACAA ATCTCTTCCC 
GGAGATCCCA ACTACCTGGT TGGAGCCAAC TGTGTGTCTG TGCTTATTGA CCATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303257.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301532.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X10, mRNA.

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CloneID	ODa40332
Clone ID Related Accession (Same CDS sequence)	XM_009303257.2 , XM_009303257.3
Accession Version	XM_009303257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CTGTCATATG GGAACAGCAT ACGGTGACTC TTCACAGGGC ACCGGGTTTT 
GGGTTTGGAA TCGCCATCTC AGGGGGCCGC GACAACCCTC ATTTTCAGAG CGGAGAGACC 
TCCATAGTGA TCTCAGATGT ACTCAAAGGG GGACCAGCAG AGGGACTTCT ACAAGAGAAC 
GATAGAGTGG TGATGGTGAA TGGCGTCTCT ATGGACAACG TGGAGCATGC CTACGCTGTT 
CAGCAGCTGA GGAAAAGTGG AAAGAATGCC AAAATTACTA TCCGCAGGAA GCGGAAGGTG 
CAGATTCCTG TAAGTCGAGG GGGAGAAAGA GAAACTATGT CGGAGCGCGA AGAGGAAGAT 
GACACAGAGG AGGATGATTA TGAGGGTCCA GCTGCTCGTG CTGGGGGAGC GAGTGGAGGC 
ACAGCAAGGA GAAATGAGCA CAGCGGCAGC ACCGGCCGGC GGGACCACAG TAATTCCAGA 
GAGAGGAGCG TTTCCCCACG CTCAGACAGA CGATCAGTTG CGTCCAACAA CCCACAGCGA 
CCTTCCAAAG TCACACTCGT CAAGTCCCGC AAAAATGAAG AATATGGTCT GCGTCTGGCC 
AGCCACATCT TTGTTAAGGA CATTTCTCCT GAGAGTCTTG CAGCGCGTGA CGGAAACATA 
CAAGAGGGAG ACGTTGTCCT GAAGATCAAT GGCACGGTGA CTGAGAATCT GTCCTTGATA 
GATGCAAAGA AGCTTATAGA GCGATCGAAA GGCAAGTTGA AAATGGTGGT GCAGAGAGAT 
GAACGAGCGA CACTCCTTAA CATTCCAGAT ATGGATGATA GCATTCCCTC AGCCAATTCA 
GACAGAGATG ACATTTCAGA AATCCATTCA CTGGCATCAG ATCATTCCCA TGAGCGCACC 
CGTCGCAGCC GATCCCGTTC CCCTGACAAG CGCTCCGAGC CCTCCGACAT CTCTGGTCAC 
TCTCCACAAC AGATGAGCAA CGGCAGGCTG ACGGGCTCTT TTCCCACCAG AAAGCTCCCG 
CTCCATAGAA GTCGAGATGA GGAGCGCATC TCTAAACCAG CGGCTCTATC CACTCCAGTT 
AAGATGGCTG AGGATGCTCT TCTGGCTCCG CCCAGCGAAC AGCCCGGGGA CAAACAGATC 
CCTCCACTGC CAGAGCCGAA ACCAGTGTAT GCACAACCTG GGCAACCCGA TGTAGATCTG 
CCAGTGAGCC CGTCTGATGC CCCTGTCCCC AGCGTCACCC ATGATGACAG CATCCTCCGC 
AGGCCCAGTA TGAAGCTGGT GAAGTTTAAG AAAGGGGAAA GTGTCGGATT GAGGCTGGCT 
GGAGGAAACG ATGTGGGGAT CTTTGTGGCT GGAGTTCTGG AGGACAGTCC TGCTGCTAAG 
GAAGGCCTGG AGGAGGGAGA CCAGATACTC AGGGTTAACA ATGTTGACTT TGCAAACATT 
ATTCGTGAGG AAGCAGTTCT GTTCCTGCTA GATCTGCCCA GAGGTGAGGA GGTCACCATC 
CTTGCGCAGA AGAAGAAAGA TGTGTACCGG CGAATAGTGG AGTCTGATGT GGGCGACTCC 
TTCTACATCA GGACACACTT TGAGTACGAG AAAGAGTCTC CTTATGGGCT GAGCTTTAAT 
AAAGGAGAGG TGTTCAGAGT GGTGGACACT CTCTACAATG GCAAACTGGG CTCCTGGCTG 
GCTATCCGCA TCGGGAAGAA CCACCAGGAG GTGGAAAGAG GCATCATTCC CAACAAGAAC 
AGGGCGGAAC AGTTATCAAG CGTACAGTAC ACACTCCCCA AGACTGCAGG TGGTGATCGT 
GCTGATTTCT GGAGGTTCAG AGGCCTGCGC AGTTCAAAGA GGAACTTGCG CAAGAGTAGA 
GAAGATCTGT CAGCTCAGCC CGTTCAGACC AAGTTCCCTG CTTATGAAAG GGTGGTTCTT 
AGAGAGGCTG GTTTCCTGAG ACCTATTGTG ATCTTTGGAC CAATCGCAGA TGTGGCACGA 
GACAAACTGG CAAGAGAGGT GCCTGACCTA TTCGAGCTCG CAAAGACACA GCTCCCAGAT 
GGAAGGGAAA AGAGTGAACC TAGAGATGCC GGCACTGACC AGCGCAGCTC GGGGATCATA 
CGCCTTCACA CTATCAAACA GATAATCGAC CGGGATAAGC ATGCAGTGCT GGACATTACT 
CCTAACGCAG TGGATCGGCT GAACTATGCT CAGTGGTATC CCATCGTAGT GTTCTTAAAT 
CCTGACAGCA AGCAGGGTGT GAAGAACATG AGGACCAGAC TGTGTTCCGA GTCCAGGAAA 
AGTGCCAGAA AACTCCACGA GCGCGCACTC AAACTGAGGA AGAACAACCA TCATCTCTTC 
ACAGCCACTA TTAACATGAA TAACATGAAT GATGGCTGGT ATGGAGCACT GAAGGACACC 
ATTCAGCAGC AGCAGAACCA GCTGGTGTGG GTCTCAGAGG GCAAGGCGGA CGGTTCACAT 
GATGATGACC TGGACCTTCA CGATGACCGT CTGTCCTATT TATCGGCCCC AGGCAGTGAG 
TACAGTATGT ACAGCACGGA CAGCCGCCAC ACCTCAGATT ACGAGGACAC GGACACCGAG 
GGTGGCGCCT ACACTGACCA GGAACTGGAC GAAACCCTGA ACGATGAGGT CGGACCACCC 
AGCGAACCAG CCATCACTCG CTCCTCAGAA CCTGTCCGCG AAGACCCGCC CGTCATCCAG 
GAGCCCCCCG GATATGGAGG ATATCAGCCC GAACCCATCA ACCGCATCGA CCCCGCGGGG 
TTCAAGGCCC CTGCACCTCA GCAGAAAGCA GAGGCTGCTC TCCCCTCGCA GCCTGAGCCA 
CTGGCAGAGA CAGCGCCCCC TGCTGTTCAT GTAAATGTAG GGGCCCCGAG TGCCAAGGAT 
CTCACTCAGG GGGAAATCAG CCCCAAGGCT GGCTCTCTCT TCCCACCTGC CCCTGAGCTT 
ATCCAACCCC CGTCTCCTGA CCCCAACCAA TCCGGACAGC CCGGGTCAGA GTCCAAGATG 
TACAAGAAGG ATATCTACCG ACCCAACGAA CCGGTGCAGA TAAGCCAAAA CCCTATGCAG 
CCCAACTACT ACCAGGAAGA CAAGCCATAC AGAGATTATG ACCACCAGCC CTACCGCTAT 
GATGGAGGCT ACATGGAACC AAAGGCTCGT AACTTTGACC CACATTTTGA GGACAGCGTG 
CCTCCATATA ATGATCAATG GGGTCAGTAT GACCACACCT CTGGGTATGA ACCTCAAACG 
CTCTACGAGG ATGGTCTGAA CCGCTTGTAT GGTCACCCTC AGTCACGAAA CGACACCGGC 
TACGACACAG GTCGACCTCG ATATGGCAAA CCAACCTCTG GACCAATCCG ACACGATGAA 
CCACCACCAT CACGCCCACA ATATGACACT CAACAAACCT CTCGTTCCCC AGAACCGCCC 
AAACAACAAT ACTACGACCC TTCCCAAAGG CCTTCCTACA CTCAAGCTCC ACAAAAAGGC 
TATATGAACT CTGAAGCACC GGTAACGCCA CCCAAACCTG AGTCGCTCTC ACCGCCTTTG 
GAGCCTGCTC TTCCTCCTCC ACCTCCTCCA GCCGAAGCAG AGGATGATCC CGCCATGAAG 
CCCCAGTCGG TGCTCACCAG GGTGAAGATG TTCGAGAACA AGCGATCGGT GTCTGTAGAC 
AGAGCCAGAG AGTCTGCAGA CTCTGGTATT CGGCCTGTGG ACATGGTGCC TAAACCTGGC 
GCTGCCTCCG TGCCCAAAGC CAATTCTTTA AGTAACCTGG ATCAAGAGAA GACCTTTAGA 
ACTCCAGAGC CCCAGCAGCC ACAGCCTAAA GTAGATGATA TCATTCGTTC AAATAACAAT 
GACCCTGATG AGGATGAAGA ATATTACAGG AAACAACTGT CCTACTTCGA CCGCAGGAGC 
TTCGACAAAC CCTCCACACA GCTGACACCT GCCATCAAAC CAGCCCAACC CCAAACTCAA 
CCAGCACACT ATCCCAGAGC AGAGTCAGTG GAGAAAATCA ACCCAGCTGC TCCATCAAAC 
CCAGCTGTCC CACCACCAAC CCTGCCTAAA CCTGCAGCAA CCCCACCTCT CGACCCTCAC 
GGATCTCCCA AAGTAAAGCC TCCTAGTCAT GAAGACACAG TCCAGAGCAC TTACCTGCCT 
CACAAAAGCT TCCCAGAGAA ATCCCCCGTC AACGGCACCG GCGAACCTCC AAAGACCAAC 
AGCACTGCCA CGTCCAGCTA CAACCGTTTT GTGCCCAAGC CGTACACCAC CTCCGCAAGA 
CCCTTCGAGC GCAAGTTTGA CAGTCCCAAG TTCAATCACA ATCTCCTCCC TAATGACTCC 
CAAAACAAGA CGGATAATGT GAAAGCTCCC ATCACCGCTA AACCACAGAG CTCACCCCAG 
CCGGCAGACC ACGACAGCGG CCTGGACACC TTCACACGCA CCATGGACCA CAAACCCAAA 
TACCAGCAGA ACAACATCAA CGCCGTACCC AAGGCAATAC CTGTCAGCCC GAGTGCTTTG 
GAGGATGATG ACGATGATGA CAGCCACACG GTGGTGGCGA CAGCGCGGGG TATTTTCAAC 
TCTAATGGTG GGGTCCTGAG CTCCATTGAG ACAGGAGTAA GCATTATTAT CCCACAGGGA 
GCCATCCCTG ATGGCGTGGA GCAAGAGATC TACTTTAAAG TGTGTCGGGA TAACAGCATC 
CTGCCACCTC TGGACAAAGA GAAAGGAGAA ACTCTGTTGA GTCCTCTGGT GATGTGTGGT 
CCTCACGGTC TGAAATTCCT GAAGCCAGTG GAGCTCCGCT TACCTCACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGATCCGAAG 
AGTTGGCAGA ACAAATCTCT TCCCGGAGAT CCCAACTACC TGGTTGGAGC CAACTGTGTG 
TCTGTGCTTA TTGACCATTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301532.1
CDS	338..5281
Translation	MEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303257.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303257.2

ORF Insert Sequence:

ATGGAGGAAA CTGTCATATG GGAACAGCAT ACGGTGACTC TTCACAGGGC ACCGGGTTTT 
GGGTTTGGAA TCGCCATCTC AGGGGGCCGC GACAACCCTC ATTTTCAGAG CGGAGAGACC 
TCCATAGTGA TCTCAGATGT ACTCAAAGGG GGACCAGCAG AGGGACTTCT ACAAGAGAAC 
GATAGAGTGG TGATGGTGAA TGGCGTCTCT ATGGACAACG TGGAGCATGC CTACGCTGTT 
CAGCAGCTGA GGAAAAGTGG AAAGAATGCC AAAATTACTA TCCGCAGGAA GCGGAAGGTG 
CAGATTCCTG TAAGTCGAGG GGGAGAAAGA GAAACTATGT CGGAGCGCGA AGAGGAAGAT 
GACACAGAGG AGGATGATTA TGAGGGTCCA GCTGCTCGTG CTGGGGGAGC GAGTGGAGGC 
ACAGCAAGGA GAAATGAGCA CAGCGGCAGC ACCGGCCGGC GGGACCACAG TAATTCCAGA 
GAGAGGAGCG TTTCCCCACG CTCAGACAGA CGATCAGTTG CGTCCAACAA CCCACAGCGA 
CCTTCCAAAG TCACACTCGT CAAGTCCCGC AAAAATGAAG AATATGGTCT GCGTCTGGCC 
AGCCACATCT TTGTTAAGGA CATTTCTCCT GAGAGTCTTG CAGCGCGTGA CGGAAACATA 
CAAGAGGGAG ACGTTGTCCT GAAGATCAAT GGCACGGTGA CTGAGAATCT GTCCTTGATA 
GATGCAAAGA AGCTTATAGA GCGATCGAAA GGCAAGTTGA AAATGGTGGT GCAGAGAGAT 
GAACGAGCGA CACTCCTTAA CATTCCAGAT ATGGATGATA GCATTCCCTC AGCCAATTCA 
GACAGAGATG ACATTTCAGA AATCCATTCA CTGGCATCAG ATCATTCCCA TGAGCGCACC 
CGTCGCAGCC GATCCCGTTC CCCTGACAAG CGCTCCGAGC CCTCCGACAT CTCTGGTCAC 
TCTCCACAAC AGATGAGCAA CGGCAGGCTG ACGGGCTCTT TTCCCACCAG AAAGCTCCCG 
CTCCATAGAA GTCGAGATGA GGAGCGCATC TCTAAACCAG CGGCTCTATC CACTCCAGTT 
AAGATGGCTG AGGATGCTCT TCTGGCTCCG CCCAGCGAAC AGCCCGGGGA CAAACAGATC 
CCTCCACTGC CAGAGCCGAA ACCAGTGTAT GCACAACCTG GGCAACCCGA TGTAGATCTG 
CCAGTGAGCC CGTCTGATGC CCCTGTCCCC AGCGTCACCC ATGATGACAG CATCCTCCGC 
AGGCCCAGTA TGAAGCTGGT GAAGTTTAAG AAAGGGGAAA GTGTCGGATT GAGGCTGGCT 
GGAGGAAACG ATGTGGGGAT CTTTGTGGCT GGAGTTCTGG AGGACAGTCC TGCTGCTAAG 
GAAGGCCTGG AGGAGGGAGA CCAGATACTC AGGGTTAACA ATGTTGACTT TGCAAACATT 
ATTCGTGAGG AAGCAGTTCT GTTCCTGCTA GATCTGCCCA GAGGTGAGGA GGTCACCATC 
CTTGCGCAGA AGAAGAAAGA TGTGTACCGG CGAATAGTGG AGTCTGATGT GGGCGACTCC 
TTCTACATCA GGACACACTT TGAGTACGAG AAAGAGTCTC CTTATGGGCT GAGCTTTAAT 
AAAGGAGAGG TGTTCAGAGT GGTGGACACT CTCTACAATG GCAAACTGGG CTCCTGGCTG 
GCTATCCGCA TCGGGAAGAA CCACCAGGAG GTGGAAAGAG GCATCATTCC CAACAAGAAC 
AGGGCGGAAC AGTTATCAAG CGTACAGTAC ACACTCCCCA AGACTGCAGG TGGTGATCGT 
GCTGATTTCT GGAGGTTCAG AGGCCTGCGC AGTTCAAAGA GGAACTTGCG CAAGAGTAGA 
GAAGATCTGT CAGCTCAGCC CGTTCAGACC AAGTTCCCTG CTTATGAAAG GGTGGTTCTT 
AGAGAGGCTG GTTTCCTGAG ACCTATTGTG ATCTTTGGAC CAATCGCAGA TGTGGCACGA 
GACAAACTGG CAAGAGAGGT GCCTGACCTA TTCGAGCTCG CAAAGACACA GCTCCCAGAT 
GGAAGGGAAA AGAGTGAACC TAGAGATGCC GGCACTGACC AGCGCAGCTC GGGGATCATA 
CGCCTTCACA CTATCAAACA GATAATCGAC CGGGATAAGC ATGCAGTGCT GGACATTACT 
CCTAACGCAG TGGATCGGCT GAACTATGCT CAGTGGTATC CCATCGTAGT GTTCTTAAAT 
CCTGACAGCA AGCAGGGTGT GAAGAACATG AGGACCAGAC TGTGTTCCGA GTCCAGGAAA 
AGTGCCAGAA AACTCCACGA GCGCGCACTC AAACTGAGGA AGAACAACCA TCATCTCTTC 
ACAGCCACTA TTAACATGAA TAACATGAAT GATGGCTGGT ATGGAGCACT GAAGGACACC 
ATTCAGCAGC AGCAGAACCA GCTGGTGTGG GTCTCAGAGG GCAAGGCGGA CGGTTCACAT 
GATGATGACC TGGACCTTCA CGATGACCGT CTGTCCTATT TATCGGCCCC AGGCAGTGAG 
TACAGTATGT ACAGCACGGA CAGCCGCCAC ACCTCAGATT ACGAGGACAC GGACACCGAG 
GGTGGCGCCT ACACTGACCA GGAACTGGAC GAAACCCTGA ACGATGAGGT CGGACCACCC 
AGCGAACCAG CCATCACTCG CTCCTCAGAA CCTGTCCGCG AAGACCCGCC CGTCATCCAG 
GAGCCCCCCG GATATGGAGG ATATCAGCCC GAACCCATCA ACCGCATCGA CCCCGCGGGG 
TTCAAGGCCC CTGCACCTCA GCAGAAAGCA GAGGCTGCTC TCCCCTCGCA GCCTGAGCCA 
CTGGCAGAGA CAGCGCCCCC TGCTGTTCAT GTAAATGTAG GGGCCCCGAG TGCCAAGGAT 
CTCACTCAGG GGGAAATCAG CCCCAAGGCT GGCTCTCTCT TCCCACCTGC CCCTGAGCTT 
ATCCAACCCC CGTCTCCTGA CCCCAACCAA TCCGGACAGC CCGGGTCAGA GTCCAAGATG 
TACAAGAAGG ATATCTACCG ACCCAACGAA CCGGTGCAGA TAAGCCAAAA CCCTATGCAG 
CCCAACTACT ACCAGGAAGA CAAGCCATAC AGAGATTATG ACCACCAGCC CTACCGCTAT 
GATGGAGGCT ACATGGAACC AAAGGCTCGT AACTTTGACC CACATTTTGA GGACAGCGTG 
CCTCCATATA ATGATCAATG GGGTCAGTAT GACCACACCT CTGGGTATGA ACCTCAAACG 
CTCTACGAGG ATGGTCTGAA CCGCTTGTAT GGTCACCCTC AGTCACGAAA CGACACCGGC 
TACGACACAG GTCGACCTCG ATATGGCAAA CCAACCTCTG GACCAATCCG ACACGATGAA 
CCACCACCAT CACGCCCACA ATATGACACT CAACAAACCT CTCGTTCCCC AGAACCGCCC 
AAACAACAAT ACTACGACCC TTCCCAAAGG CCTTCCTACA CTCAAGCTCC ACAAAAAGGC 
TATATGAACT CTGAAGCACC GGTAACGCCA CCCAAACCTG AGTCGCTCTC ACCGCCTTTG 
GAGCCTGCTC TTCCTCCTCC ACCTCCTCCA GCCGAAGCAG AGGATGATCC CGCCATGAAG 
CCCCAGTCGG TGCTCACCAG GGTGAAGATG TTCGAGAACA AGCGATCGGT GTCTGTAGAC 
AGAGCCAGAG AGTCTGCAGA CTCTGGTATT CGGCCTGTGG ACATGGTGCC TAAACCTGGC 
GCTGCCTCCG TGCCCAAAGC CAATTCTTTA AGTAACCTGG ATCAAGAGAA GACCTTTAGA 
ACTCCAGAGC CCCAGCAGCC ACAGCCTAAA GTAGATGATA TCATTCGTTC AAATAACAAT 
GACCCTGATG AGGATGAAGA ATATTACAGG AAACAACTGT CCTACTTCGA CCGCAGGAGC 
TTCGACAAAC CCTCCACACA GCTGACACCT GCCATCAAAC CAGCCCAACC CCAAACTCAA 
CCAGCACACT ATCCCAGAGC AGAGTCAGTG GAGAAAATCA ACCCAGCTGC TCCATCAAAC 
CCAGCTGTCC CACCACCAAC CCTGCCTAAA CCTGCAGCAA CCCCACCTCT CGACCCTCAC 
GGATCTCCCA AAGTAAAGCC TCCTAGTCAT GAAGACACAG TCCAGAGCAC TTACCTGCCT 
CACAAAAGCT TCCCAGAGAA ATCCCCCGTC AACGGCACCG GCGAACCTCC AAAGACCAAC 
AGCACTGCCA CGTCCAGCTA CAACCGTTTT GTGCCCAAGC CGTACACCAC CTCCGCAAGA 
CCCTTCGAGC GCAAGTTTGA CAGTCCCAAG TTCAATCACA ATCTCCTCCC TAATGACTCC 
CAAAACAAGA CGGATAATGT GAAAGCTCCC ATCACCGCTA AACCACAGAG CTCACCCCAG 
CCGGCAGACC ACGACAGCGG CCTGGACACC TTCACACGCA CCATGGACCA CAAACCCAAA 
TACCAGCAGA ACAACATCAA CGCCGTACCC AAGGCAATAC CTGTCAGCCC GAGTGCTTTG 
GAGGATGATG ACGATGATGA CAGCCACACG GTGGTGGCGA CAGCGCGGGG TATTTTCAAC 
TCTAATGGTG GGGTCCTGAG CTCCATTGAG ACAGGAGTAA GCATTATTAT CCCACAGGGA 
GCCATCCCTG ATGGCGTGGA GCAAGAGATC TACTTTAAAG TGTGTCGGGA TAACAGCATC 
CTGCCACCTC TGGACAAAGA GAAAGGAGAA ACTCTGTTGA GTCCTCTGGT GATGTGTGGT 
CCTCACGGTC TGAAATTCCT GAAGCCAGTG GAGCTCCGCT TACCTCACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGATCCGAAG 
AGTTGGCAGA ACAAATCTCT TCCCGGAGAT CCCAACTACC TGGTTGGAGC CAACTGTGTG 
TCTGTGCTTA TTGACCATTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303257.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301532.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa40332
Clone ID Related Accession (Same CDS sequence)	XM_009303257.2 , XM_009303257.3
Accession Version	XM_009303257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CTGTCATATG GGAACAGCAT ACGGTGACTC TTCACAGGGC ACCGGGTTTT 
GGGTTTGGAA TCGCCATCTC AGGGGGCCGC GACAACCCTC ATTTTCAGAG CGGAGAGACC 
TCCATAGTGA TCTCAGATGT ACTCAAAGGG GGACCAGCAG AGGGACTTCT ACAAGAGAAC 
GATAGAGTGG TGATGGTGAA TGGCGTCTCT ATGGACAACG TGGAGCATGC CTACGCTGTT 
CAGCAGCTGA GGAAAAGTGG AAAGAATGCC AAAATTACTA TCCGCAGGAA GCGGAAGGTG 
CAGATTCCTG TAAGTCGAGG GGGAGAAAGA GAAACTATGT CGGAGCGCGA AGAGGAAGAT 
GACACAGAGG AGGATGATTA TGAGGGTCCA GCTGCTCGTG CTGGGGGAGC GAGTGGAGGC 
ACAGCAAGGA GAAATGAGCA CAGCGGCAGC ACCGGCCGGC GGGACCACAG TAATTCCAGA 
GAGAGGAGCG TTTCCCCACG CTCAGACAGA CGATCAGTTG CGTCCAACAA CCCACAGCGA 
CCTTCCAAAG TCACACTCGT CAAGTCCCGC AAAAATGAAG AATATGGTCT GCGTCTGGCC 
AGCCACATCT TTGTTAAGGA CATTTCTCCT GAGAGTCTTG CAGCGCGTGA CGGAAACATA 
CAAGAGGGAG ACGTTGTCCT GAAGATCAAT GGCACGGTGA CTGAGAATCT GTCCTTGATA 
GATGCAAAGA AGCTTATAGA GCGATCGAAA GGCAAGTTGA AAATGGTGGT GCAGAGAGAT 
GAACGAGCGA CACTCCTTAA CATTCCAGAT ATGGATGATA GCATTCCCTC AGCCAATTCA 
GACAGAGATG ACATTTCAGA AATCCATTCA CTGGCATCAG ATCATTCCCA TGAGCGCACC 
CGTCGCAGCC GATCCCGTTC CCCTGACAAG CGCTCCGAGC CCTCCGACAT CTCTGGTCAC 
TCTCCACAAC AGATGAGCAA CGGCAGGCTG ACGGGCTCTT TTCCCACCAG AAAGCTCCCG 
CTCCATAGAA GTCGAGATGA GGAGCGCATC TCTAAACCAG CGGCTCTATC CACTCCAGTT 
AAGATGGCTG AGGATGCTCT TCTGGCTCCG CCCAGCGAAC AGCCCGGGGA CAAACAGATC 
CCTCCACTGC CAGAGCCGAA ACCAGTGTAT GCACAACCTG GGCAACCCGA TGTAGATCTG 
CCAGTGAGCC CGTCTGATGC CCCTGTCCCC AGCGTCACCC ATGATGACAG CATCCTCCGC 
AGGCCCAGTA TGAAGCTGGT GAAGTTTAAG AAAGGGGAAA GTGTCGGATT GAGGCTGGCT 
GGAGGAAACG ATGTGGGGAT CTTTGTGGCT GGAGTTCTGG AGGACAGTCC TGCTGCTAAG 
GAAGGCCTGG AGGAGGGAGA CCAGATACTC AGGGTTAACA ATGTTGACTT TGCAAACATT 
ATTCGTGAGG AAGCAGTTCT GTTCCTGCTA GATCTGCCCA GAGGTGAGGA GGTCACCATC 
CTTGCGCAGA AGAAGAAAGA TGTGTACCGG CGAATAGTGG AGTCTGATGT GGGCGACTCC 
TTCTACATCA GGACACACTT TGAGTACGAG AAAGAGTCTC CTTATGGGCT GAGCTTTAAT 
AAAGGAGAGG TGTTCAGAGT GGTGGACACT CTCTACAATG GCAAACTGGG CTCCTGGCTG 
GCTATCCGCA TCGGGAAGAA CCACCAGGAG GTGGAAAGAG GCATCATTCC CAACAAGAAC 
AGGGCGGAAC AGTTATCAAG CGTACAGTAC ACACTCCCCA AGACTGCAGG TGGTGATCGT 
GCTGATTTCT GGAGGTTCAG AGGCCTGCGC AGTTCAAAGA GGAACTTGCG CAAGAGTAGA 
GAAGATCTGT CAGCTCAGCC CGTTCAGACC AAGTTCCCTG CTTATGAAAG GGTGGTTCTT 
AGAGAGGCTG GTTTCCTGAG ACCTATTGTG ATCTTTGGAC CAATCGCAGA TGTGGCACGA 
GACAAACTGG CAAGAGAGGT GCCTGACCTA TTCGAGCTCG CAAAGACACA GCTCCCAGAT 
GGAAGGGAAA AGAGTGAACC TAGAGATGCC GGCACTGACC AGCGCAGCTC GGGGATCATA 
CGCCTTCACA CTATCAAACA GATAATCGAC CGGGATAAGC ATGCAGTGCT GGACATTACT 
CCTAACGCAG TGGATCGGCT GAACTATGCT CAGTGGTATC CCATCGTAGT GTTCTTAAAT 
CCTGACAGCA AGCAGGGTGT GAAGAACATG AGGACCAGAC TGTGTTCCGA GTCCAGGAAA 
AGTGCCAGAA AACTCCACGA GCGCGCACTC AAACTGAGGA AGAACAACCA TCATCTCTTC 
ACAGCCACTA TTAACATGAA TAACATGAAT GATGGCTGGT ATGGAGCACT GAAGGACACC 
ATTCAGCAGC AGCAGAACCA GCTGGTGTGG GTCTCAGAGG GCAAGGCGGA CGGTTCACAT 
GATGATGACC TGGACCTTCA CGATGACCGT CTGTCCTATT TATCGGCCCC AGGCAGTGAG 
TACAGTATGT ACAGCACGGA CAGCCGCCAC ACCTCAGATT ACGAGGACAC GGACACCGAG 
GGTGGCGCCT ACACTGACCA GGAACTGGAC GAAACCCTGA ACGATGAGGT CGGACCACCC 
AGCGAACCAG CCATCACTCG CTCCTCAGAA CCTGTCCGCG AAGACCCGCC CGTCATCCAG 
GAGCCCCCCG GATATGGAGG ATATCAGCCC GAACCCATCA ACCGCATCGA CCCCGCGGGG 
TTCAAGGCCC CTGCACCTCA GCAGAAAGCA GAGGCTGCTC TCCCCTCGCA GCCTGAGCCA 
CTGGCAGAGA CAGCGCCCCC TGCTGTTCAT GTAAATGTAG GGGCCCCGAG TGCCAAGGAT 
CTCACTCAGG GGGAAATCAG CCCCAAGGCT GGCTCTCTCT TCCCACCTGC CCCTGAGCTT 
ATCCAACCCC CGTCTCCTGA CCCCAACCAA TCCGGACAGC CCGGGTCAGA GTCCAAGATG 
TACAAGAAGG ATATCTACCG ACCCAACGAA CCGGTGCAGA TAAGCCAAAA CCCTATGCAG 
CCCAACTACT ACCAGGAAGA CAAGCCATAC AGAGATTATG ACCACCAGCC CTACCGCTAT 
GATGGAGGCT ACATGGAACC AAAGGCTCGT AACTTTGACC CACATTTTGA GGACAGCGTG 
CCTCCATATA ATGATCAATG GGGTCAGTAT GACCACACCT CTGGGTATGA ACCTCAAACG 
CTCTACGAGG ATGGTCTGAA CCGCTTGTAT GGTCACCCTC AGTCACGAAA CGACACCGGC 
TACGACACAG GTCGACCTCG ATATGGCAAA CCAACCTCTG GACCAATCCG ACACGATGAA 
CCACCACCAT CACGCCCACA ATATGACACT CAACAAACCT CTCGTTCCCC AGAACCGCCC 
AAACAACAAT ACTACGACCC TTCCCAAAGG CCTTCCTACA CTCAAGCTCC ACAAAAAGGC 
TATATGAACT CTGAAGCACC GGTAACGCCA CCCAAACCTG AGTCGCTCTC ACCGCCTTTG 
GAGCCTGCTC TTCCTCCTCC ACCTCCTCCA GCCGAAGCAG AGGATGATCC CGCCATGAAG 
CCCCAGTCGG TGCTCACCAG GGTGAAGATG TTCGAGAACA AGCGATCGGT GTCTGTAGAC 
AGAGCCAGAG AGTCTGCAGA CTCTGGTATT CGGCCTGTGG ACATGGTGCC TAAACCTGGC 
GCTGCCTCCG TGCCCAAAGC CAATTCTTTA AGTAACCTGG ATCAAGAGAA GACCTTTAGA 
ACTCCAGAGC CCCAGCAGCC ACAGCCTAAA GTAGATGATA TCATTCGTTC AAATAACAAT 
GACCCTGATG AGGATGAAGA ATATTACAGG AAACAACTGT CCTACTTCGA CCGCAGGAGC 
TTCGACAAAC CCTCCACACA GCTGACACCT GCCATCAAAC CAGCCCAACC CCAAACTCAA 
CCAGCACACT ATCCCAGAGC AGAGTCAGTG GAGAAAATCA ACCCAGCTGC TCCATCAAAC 
CCAGCTGTCC CACCACCAAC CCTGCCTAAA CCTGCAGCAA CCCCACCTCT CGACCCTCAC 
GGATCTCCCA AAGTAAAGCC TCCTAGTCAT GAAGACACAG TCCAGAGCAC TTACCTGCCT 
CACAAAAGCT TCCCAGAGAA ATCCCCCGTC AACGGCACCG GCGAACCTCC AAAGACCAAC 
AGCACTGCCA CGTCCAGCTA CAACCGTTTT GTGCCCAAGC CGTACACCAC CTCCGCAAGA 
CCCTTCGAGC GCAAGTTTGA CAGTCCCAAG TTCAATCACA ATCTCCTCCC TAATGACTCC 
CAAAACAAGA CGGATAATGT GAAAGCTCCC ATCACCGCTA AACCACAGAG CTCACCCCAG 
CCGGCAGACC ACGACAGCGG CCTGGACACC TTCACACGCA CCATGGACCA CAAACCCAAA 
TACCAGCAGA ACAACATCAA CGCCGTACCC AAGGCAATAC CTGTCAGCCC GAGTGCTTTG 
GAGGATGATG ACGATGATGA CAGCCACACG GTGGTGGCGA CAGCGCGGGG TATTTTCAAC 
TCTAATGGTG GGGTCCTGAG CTCCATTGAG ACAGGAGTAA GCATTATTAT CCCACAGGGA 
GCCATCCCTG ATGGCGTGGA GCAAGAGATC TACTTTAAAG TGTGTCGGGA TAACAGCATC 
CTGCCACCTC TGGACAAAGA GAAAGGAGAA ACTCTGTTGA GTCCTCTGGT GATGTGTGGT 
CCTCACGGTC TGAAATTCCT GAAGCCAGTG GAGCTCCGCT TACCTCACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGATCCGAAG 
AGTTGGCAGA ACAAATCTCT TCCCGGAGAT CCCAACTACC TGGTTGGAGC CAACTGTGTG 
TCTGTGCTTA TTGACCATTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301532.1
CDS	369..5312
Translation	MEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGRLTGSFPTRKLPLHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKTQLPDGREKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303257.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303257.3

ORF Insert Sequence:

ATGGAGGAAA CTGTCATATG GGAACAGCAT ACGGTGACTC TTCACAGGGC ACCGGGTTTT 
GGGTTTGGAA TCGCCATCTC AGGGGGCCGC GACAACCCTC ATTTTCAGAG CGGAGAGACC 
TCCATAGTGA TCTCAGATGT ACTCAAAGGG GGACCAGCAG AGGGACTTCT ACAAGAGAAC 
GATAGAGTGG TGATGGTGAA TGGCGTCTCT ATGGACAACG TGGAGCATGC CTACGCTGTT 
CAGCAGCTGA GGAAAAGTGG AAAGAATGCC AAAATTACTA TCCGCAGGAA GCGGAAGGTG 
CAGATTCCTG TAAGTCGAGG GGGAGAAAGA GAAACTATGT CGGAGCGCGA AGAGGAAGAT 
GACACAGAGG AGGATGATTA TGAGGGTCCA GCTGCTCGTG CTGGGGGAGC GAGTGGAGGC 
ACAGCAAGGA GAAATGAGCA CAGCGGCAGC ACCGGCCGGC GGGACCACAG TAATTCCAGA 
GAGAGGAGCG TTTCCCCACG CTCAGACAGA CGATCAGTTG CGTCCAACAA CCCACAGCGA 
CCTTCCAAAG TCACACTCGT CAAGTCCCGC AAAAATGAAG AATATGGTCT GCGTCTGGCC 
AGCCACATCT TTGTTAAGGA CATTTCTCCT GAGAGTCTTG CAGCGCGTGA CGGAAACATA 
CAAGAGGGAG ACGTTGTCCT GAAGATCAAT GGCACGGTGA CTGAGAATCT GTCCTTGATA 
GATGCAAAGA AGCTTATAGA GCGATCGAAA GGCAAGTTGA AAATGGTGGT GCAGAGAGAT 
GAACGAGCGA CACTCCTTAA CATTCCAGAT ATGGATGATA GCATTCCCTC AGCCAATTCA 
GACAGAGATG ACATTTCAGA AATCCATTCA CTGGCATCAG ATCATTCCCA TGAGCGCACC 
CGTCGCAGCC GATCCCGTTC CCCTGACAAG CGCTCCGAGC CCTCCGACAT CTCTGGTCAC 
TCTCCACAAC AGATGAGCAA CGGCAGGCTG ACGGGCTCTT TTCCCACCAG AAAGCTCCCG 
CTCCATAGAA GTCGAGATGA GGAGCGCATC TCTAAACCAG CGGCTCTATC CACTCCAGTT 
AAGATGGCTG AGGATGCTCT TCTGGCTCCG CCCAGCGAAC AGCCCGGGGA CAAACAGATC 
CCTCCACTGC CAGAGCCGAA ACCAGTGTAT GCACAACCTG GGCAACCCGA TGTAGATCTG 
CCAGTGAGCC CGTCTGATGC CCCTGTCCCC AGCGTCACCC ATGATGACAG CATCCTCCGC 
AGGCCCAGTA TGAAGCTGGT GAAGTTTAAG AAAGGGGAAA GTGTCGGATT GAGGCTGGCT 
GGAGGAAACG ATGTGGGGAT CTTTGTGGCT GGAGTTCTGG AGGACAGTCC TGCTGCTAAG 
GAAGGCCTGG AGGAGGGAGA CCAGATACTC AGGGTTAACA ATGTTGACTT TGCAAACATT 
ATTCGTGAGG AAGCAGTTCT GTTCCTGCTA GATCTGCCCA GAGGTGAGGA GGTCACCATC 
CTTGCGCAGA AGAAGAAAGA TGTGTACCGG CGAATAGTGG AGTCTGATGT GGGCGACTCC 
TTCTACATCA GGACACACTT TGAGTACGAG AAAGAGTCTC CTTATGGGCT GAGCTTTAAT 
AAAGGAGAGG TGTTCAGAGT GGTGGACACT CTCTACAATG GCAAACTGGG CTCCTGGCTG 
GCTATCCGCA TCGGGAAGAA CCACCAGGAG GTGGAAAGAG GCATCATTCC CAACAAGAAC 
AGGGCGGAAC AGTTATCAAG CGTACAGTAC ACACTCCCCA AGACTGCAGG TGGTGATCGT 
GCTGATTTCT GGAGGTTCAG AGGCCTGCGC AGTTCAAAGA GGAACTTGCG CAAGAGTAGA 
GAAGATCTGT CAGCTCAGCC CGTTCAGACC AAGTTCCCTG CTTATGAAAG GGTGGTTCTT 
AGAGAGGCTG GTTTCCTGAG ACCTATTGTG ATCTTTGGAC CAATCGCAGA TGTGGCACGA 
GACAAACTGG CAAGAGAGGT GCCTGACCTA TTCGAGCTCG CAAAGACACA GCTCCCAGAT 
GGAAGGGAAA AGAGTGAACC TAGAGATGCC GGCACTGACC AGCGCAGCTC GGGGATCATA 
CGCCTTCACA CTATCAAACA GATAATCGAC CGGGATAAGC ATGCAGTGCT GGACATTACT 
CCTAACGCAG TGGATCGGCT GAACTATGCT CAGTGGTATC CCATCGTAGT GTTCTTAAAT 
CCTGACAGCA AGCAGGGTGT GAAGAACATG AGGACCAGAC TGTGTTCCGA GTCCAGGAAA 
AGTGCCAGAA AACTCCACGA GCGCGCACTC AAACTGAGGA AGAACAACCA TCATCTCTTC 
ACAGCCACTA TTAACATGAA TAACATGAAT GATGGCTGGT ATGGAGCACT GAAGGACACC 
ATTCAGCAGC AGCAGAACCA GCTGGTGTGG GTCTCAGAGG GCAAGGCGGA CGGTTCACAT 
GATGATGACC TGGACCTTCA CGATGACCGT CTGTCCTATT TATCGGCCCC AGGCAGTGAG 
TACAGTATGT ACAGCACGGA CAGCCGCCAC ACCTCAGATT ACGAGGACAC GGACACCGAG 
GGTGGCGCCT ACACTGACCA GGAACTGGAC GAAACCCTGA ACGATGAGGT CGGACCACCC 
AGCGAACCAG CCATCACTCG CTCCTCAGAA CCTGTCCGCG AAGACCCGCC CGTCATCCAG 
GAGCCCCCCG GATATGGAGG ATATCAGCCC GAACCCATCA ACCGCATCGA CCCCGCGGGG 
TTCAAGGCCC CTGCACCTCA GCAGAAAGCA GAGGCTGCTC TCCCCTCGCA GCCTGAGCCA 
CTGGCAGAGA CAGCGCCCCC TGCTGTTCAT GTAAATGTAG GGGCCCCGAG TGCCAAGGAT 
CTCACTCAGG GGGAAATCAG CCCCAAGGCT GGCTCTCTCT TCCCACCTGC CCCTGAGCTT 
ATCCAACCCC CGTCTCCTGA CCCCAACCAA TCCGGACAGC CCGGGTCAGA GTCCAAGATG 
TACAAGAAGG ATATCTACCG ACCCAACGAA CCGGTGCAGA TAAGCCAAAA CCCTATGCAG 
CCCAACTACT ACCAGGAAGA CAAGCCATAC AGAGATTATG ACCACCAGCC CTACCGCTAT 
GATGGAGGCT ACATGGAACC AAAGGCTCGT AACTTTGACC CACATTTTGA GGACAGCGTG 
CCTCCATATA ATGATCAATG GGGTCAGTAT GACCACACCT CTGGGTATGA ACCTCAAACG 
CTCTACGAGG ATGGTCTGAA CCGCTTGTAT GGTCACCCTC AGTCACGAAA CGACACCGGC 
TACGACACAG GTCGACCTCG ATATGGCAAA CCAACCTCTG GACCAATCCG ACACGATGAA 
CCACCACCAT CACGCCCACA ATATGACACT CAACAAACCT CTCGTTCCCC AGAACCGCCC 
AAACAACAAT ACTACGACCC TTCCCAAAGG CCTTCCTACA CTCAAGCTCC ACAAAAAGGC 
TATATGAACT CTGAAGCACC GGTAACGCCA CCCAAACCTG AGTCGCTCTC ACCGCCTTTG 
GAGCCTGCTC TTCCTCCTCC ACCTCCTCCA GCCGAAGCAG AGGATGATCC CGCCATGAAG 
CCCCAGTCGG TGCTCACCAG GGTGAAGATG TTCGAGAACA AGCGATCGGT GTCTGTAGAC 
AGAGCCAGAG AGTCTGCAGA CTCTGGTATT CGGCCTGTGG ACATGGTGCC TAAACCTGGC 
GCTGCCTCCG TGCCCAAAGC CAATTCTTTA AGTAACCTGG ATCAAGAGAA GACCTTTAGA 
ACTCCAGAGC CCCAGCAGCC ACAGCCTAAA GTAGATGATA TCATTCGTTC AAATAACAAT 
GACCCTGATG AGGATGAAGA ATATTACAGG AAACAACTGT CCTACTTCGA CCGCAGGAGC 
TTCGACAAAC CCTCCACACA GCTGACACCT GCCATCAAAC CAGCCCAACC CCAAACTCAA 
CCAGCACACT ATCCCAGAGC AGAGTCAGTG GAGAAAATCA ACCCAGCTGC TCCATCAAAC 
CCAGCTGTCC CACCACCAAC CCTGCCTAAA CCTGCAGCAA CCCCACCTCT CGACCCTCAC 
GGATCTCCCA AAGTAAAGCC TCCTAGTCAT GAAGACACAG TCCAGAGCAC TTACCTGCCT 
CACAAAAGCT TCCCAGAGAA ATCCCCCGTC AACGGCACCG GCGAACCTCC AAAGACCAAC 
AGCACTGCCA CGTCCAGCTA CAACCGTTTT GTGCCCAAGC CGTACACCAC CTCCGCAAGA 
CCCTTCGAGC GCAAGTTTGA CAGTCCCAAG TTCAATCACA ATCTCCTCCC TAATGACTCC 
CAAAACAAGA CGGATAATGT GAAAGCTCCC ATCACCGCTA AACCACAGAG CTCACCCCAG 
CCGGCAGACC ACGACAGCGG CCTGGACACC TTCACACGCA CCATGGACCA CAAACCCAAA 
TACCAGCAGA ACAACATCAA CGCCGTACCC AAGGCAATAC CTGTCAGCCC GAGTGCTTTG 
GAGGATGATG ACGATGATGA CAGCCACACG GTGGTGGCGA CAGCGCGGGG TATTTTCAAC 
TCTAATGGTG GGGTCCTGAG CTCCATTGAG ACAGGAGTAA GCATTATTAT CCCACAGGGA 
GCCATCCCTG ATGGCGTGGA GCAAGAGATC TACTTTAAAG TGTGTCGGGA TAACAGCATC 
CTGCCACCTC TGGACAAAGA GAAAGGAGAA ACTCTGTTGA GTCCTCTGGT GATGTGTGGT 
CCTCACGGTC TGAAATTCCT GAAGCCAGTG GAGCTCCGCT TACCTCACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGATCCGAAG 
AGTTGGCAGA ACAAATCTCT TCCCGGAGAT CCCAACTACC TGGTTGGAGC CAACTGTGTG 
TCTGTGCTTA TTGACCATTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303258.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301533.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa40333
Clone ID Related Accession (Same CDS sequence)	XM_009303258.3 , XM_009303258.2
Accession Version	XM_009303258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301533.1
CDS	383..5461
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303258.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303258.3

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1a ( XM_009303258.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301533.1 Danio rerio tight junction protein 1a (tjp1a), transcript variant X11, mRNA.

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CloneID	ODa40333
Clone ID Related Accession (Same CDS sequence)	XM_009303258.3 , XM_009303258.2
Accession Version	XM_009303258.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301533.1
CDS	409..5487
Translation	MKYQKYITVMQMAMGVTASNKDNCIPPKRQLWVPPASDAPAASAAATSPAAAATASQGKPSLRRIKGRIHRSKSLDSMDLLDCNSTTMEETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNGVSMDNVEHAYAVQQLRKSGKNAKITIRRKRKVQIPVSRGGERETMSEREEEDDTEEDDYEGPAARAGGASGGTARRNEHSGSTGRRDHSNSRERSVSPRSDRRSVASNNPQRPSKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDMDDSIPSANSDRDDISEIHSLASDHSHERTRRSRSRSPDKRSEPSDISGHSPQQMSNGSHRSRDEERISKPAALSTPVKMAEDALLAPPSEQPGDKQIPPLPEPKPVYAQPGQPDVDLPVSPSDAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPRGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPIVIFGPIADVARDKLAREVPDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCSESRKSARKLHERALKLRKNNHHLFTATINMNNMNDGWYGALKDTIQQQQNQLVWVSEGKADGSHDDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGPPSEPAITRSSEPVREDPPVIQEPPGYGGYQPEPINRIDPAGFKAPAPQQKAEAALPSQPEPLAETAPPAVHVNVGAPSAKDLTQGEISPKAGSLFPPAPELIQPPSPDPNQSGQPGSESKMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEPKARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRPRYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPSYTQAPQKGYMNSEAPVTPPKPESLSPPLEPALPPPPPPAEAEDDPAMKPQSVLTRVKMFENKRSVSVDRARESADSGIRPVDMVPKPGAASVPKANSLSNLDQEKTFRTPEPQQPQPKVDDIIRSNNNDPDEDEEYYRKQLSYFDRRSFDKPSTQLTPAIKPAQPQTQPAHYPRAESVEKINPAAPSNPAVPPPTLPKPAATPPLDPHGSPKVKPPSHEDTVQSTYLPHKSFPEKSPVNGTGEPPKTNSTATSSYNRFVPKPYTTSARPFERKFDSPKFNHNLLPNDSQNKTDNVKAPITAKPQSSPQPADHDSGLDTFTRTMDHKPKYQQNNINAVPKAIPVSPSALEDDDDDDSHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPDGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKSWQNKSLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009303258.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1a (tjp1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303258.2

ORF Insert Sequence:

ATGAAGTACC AGAAATACAT AACAGTGATG CAGATGGCCA TGGGAGTGAC AGCCTCCAAT 
AAAGACAACT GCATCCCTCC GAAGAGACAG CTGTGGGTGC CACCTGCCTC AGATGCCCCT 
GCCGCCTCTG CTGCTGCCAC GTCACCTGCT GCTGCTGCGA CTGCCAGTCA GGGCAAACCA 
TCACTGCGCA GGATCAAGGG CCGCATCCAC AGAAGCAAGA GCCTGGACAG CATGGATCTA 
CTGGACTGCA ATAGTACAAC AATGGAGGAA ACTGTCATAT GGGAACAGCA TACGGTGACT 
CTTCACAGGG CACCGGGTTT TGGGTTTGGA ATCGCCATCT CAGGGGGCCG CGACAACCCT 
CATTTTCAGA GCGGAGAGAC CTCCATAGTG ATCTCAGATG TACTCAAAGG GGGACCAGCA 
GAGGGACTTC TACAAGAGAA CGATAGAGTG GTGATGGTGA ATGGCGTCTC TATGGACAAC 
GTGGAGCATG CCTACGCTGT TCAGCAGCTG AGGAAAAGTG GAAAGAATGC CAAAATTACT 
ATCCGCAGGA AGCGGAAGGT GCAGATTCCT GTAAGTCGAG GGGGAGAAAG AGAAACTATG 
TCGGAGCGCG AAGAGGAAGA TGACACAGAG GAGGATGATT ATGAGGGTCC AGCTGCTCGT 
GCTGGGGGAG CGAGTGGAGG CACAGCAAGG AGAAATGAGC ACAGCGGCAG CACCGGCCGG 
CGGGACCACA GTAATTCCAG AGAGAGGAGC GTTTCCCCAC GCTCAGACAG ACGATCAGTT 
GCGTCCAACA ACCCACAGCG ACCTTCCAAA GTCACACTCG TCAAGTCCCG CAAAAATGAA 
GAATATGGTC TGCGTCTGGC CAGCCACATC TTTGTTAAGG ACATTTCTCC TGAGAGTCTT 
GCAGCGCGTG ACGGAAACAT ACAAGAGGGA GACGTTGTCC TGAAGATCAA TGGCACGGTG 
ACTGAGAATC TGTCCTTGAT AGATGCAAAG AAGCTTATAG AGCGATCGAA AGGCAAGTTG 
AAAATGGTGG TGCAGAGAGA TGAACGAGCG ACACTCCTTA ACATTCCAGA TATGGATGAT 
AGCATTCCCT CAGCCAATTC AGACAGAGAT GACATTTCAG AAATCCATTC ACTGGCATCA 
GATCATTCCC ATGAGCGCAC CCGTCGCAGC CGATCCCGTT CCCCTGACAA GCGCTCCGAG 
CCCTCCGACA TCTCTGGTCA CTCTCCACAA CAGATGAGCA ACGGCAGCCA TAGAAGTCGA 
GATGAGGAGC GCATCTCTAA ACCAGCGGCT CTATCCACTC CAGTTAAGAT GGCTGAGGAT 
GCTCTTCTGG CTCCGCCCAG CGAACAGCCC GGGGACAAAC AGATCCCTCC ACTGCCAGAG 
CCGAAACCAG TGTATGCACA ACCTGGGCAA CCCGATGTAG ATCTGCCAGT GAGCCCGTCT 
GATGCCCCTG TCCCCAGCGT CACCCATGAT GACAGCATCC TCCGGCCCAG TATGAAGCTG 
GTGAAGTTTA AGAAAGGGGA AAGTGTCGGA TTGAGGCTGG CTGGAGGAAA CGATGTGGGG 
ATCTTTGTGG CTGGAGTTCT GGAGGACAGT CCTGCTGCTA AGGAAGGCCT GGAGGAGGGA 
GACCAGATAC TCAGGGTTAA CAATGTTGAC TTTGCAAACA TTATTCGTGA GGAAGCAGTT 
CTGTTCCTGC TAGATCTGCC CAGAGGTGAG GAGGTCACCA TCCTTGCGCA GAAGAAGAAA 
GATGTGTACC GGCGAATAGT GGAGTCTGAT GTGGGCGACT CCTTCTACAT CAGGACACAC 
TTTGAGTACG AGAAAGAGTC TCCTTATGGG CTGAGCTTTA ATAAAGGAGA GGTGTTCAGA 
GTGGTGGACA CTCTCTACAA TGGCAAACTG GGCTCCTGGC TGGCTATCCG CATCGGGAAG 
AACCACCAGG AGGTGGAAAG AGGCATCATT CCCAACAAGA ACAGGGCGGA ACAGTTATCA 
AGCGTACAGT ACACACTCCC CAAGACTGCA GGTGGTGATC GTGCTGATTT CTGGAGGTTC 
AGAGGCCTGC GCAGTTCAAA GAGGAACTTG CGCAAGAGTA GAGAAGATCT GTCAGCTCAG 
CCCGTTCAGA CCAAGTTCCC TGCTTATGAA AGGGTGGTTC TTAGAGAGGC TGGTTTCCTG 
AGACCTATTG TGATCTTTGG ACCAATCGCA GATGTGGCAC GAGACAAACT GGCAAGAGAG 
GTGCCTGACC TATTCGAGCT CGCAAAGAGT GAACCTAGAG ATGCCGGCAC TGACCAGCGC 
AGCTCGGGGA TCATACGCCT TCACACTATC AAACAGATAA TCGACCGGGA TAAGCATGCA 
GTGCTGGACA TTACTCCTAA CGCAGTGGAT CGGCTGAACT ATGCTCAGTG GTATCCCATC 
GTAGTGTTCT TAAATCCTGA CAGCAAGCAG GGTGTGAAGA ACATGAGGAC CAGACTGTGT 
TCCGAGTCCA GGAAAAGTGC CAGAAAACTC CACGAGCGCG CACTCAAACT GAGGAAGAAC 
AACCATCATC TCTTCACAGC CACTATTAAC ATGAATAACA TGAATGATGG CTGGTATGGA 
GCACTGAAGG ACACCATTCA GCAGCAGCAG AACCAGCTGG TGTGGGTCTC AGAGGGCAAG 
GCGGACGGTT CACATGATGA TGACCTGGAC CTTCACGATG ACCGTCTGTC CTATTTATCG 
GCCCCAGGCA GTGAGTACAG TATGTACAGC ACGGACAGCC GCCACACCTC AGATTACGAG 
GACACGGACA CCGAGGGTGG CGCCTACACT GACCAGGAAC TGGACGAAAC CCTGAACGAT 
GAGGTCGGAC CACCCAGCGA ACCAGCCATC ACTCGCTCCT CAGAACCTGT CCGCGAAGAC 
CCGCCCGTCA TCCAGGAGCC CCCCGGATAT GGAGGATATC AGCCCGAACC CATCAACCGC 
ATCGACCCCG CGGGGTTCAA GGCCCCTGCA CCTCAGCAGA AAGCAGAGGC TGCTCTCCCC 
TCGCAGCCTG AGCCACTGGC AGAGACAGCG CCCCCTGCTG TTCATGTAAA TGTAGGGGCC 
CCGAGTGCCA AGGATCTCAC TCAGGGGGAA ATCAGCCCCA AGGCTGGCTC TCTCTTCCCA 
CCTGCCCCTG AGCTTATCCA ACCCCCGTCT CCTGACCCCA ACCAATCCGG ACAGCCCGGG 
TCAGAGTCCA AGATGTACAA GAAGGATATC TACCGACCCA ACGAACCGGT GCAGATAAGC 
CAAAACCCTA TGCAGCCCAA CTACTACCAG GAAGACAAGC CATACAGAGA TTATGACCAC 
CAGCCCTACC GCTATGATGG AGGCTACATG GAACCAAAGG CTCGTAACTT TGACCCACAT 
TTTGAGGACA GCGTGCCTCC ATATAATGAT CAATGGGGTC AGTATGACCA CACCTCTGGG 
TATGAACCTC AAACGCTCTA CGAGGATGGT CTGAACCGCT TGTATGGTCA CCCTCAGTCA 
CGAAACGACA CCGGCTACGA CACAGGTCGA CCTCGATATG GCAAACCAAC CTCTGGACCA 
ATCCGACACG ATGAACCACC ACCATCACGC CCACAATATG ACACTCAACA AACCTCTCGT 
TCCCCAGAAC CGCCCAAACA ACAATACTAC GACCCTTCCC AAAGGCCTTC CTACACTCAA 
GCTCCACAAA AAGGCTATAT GAACTCTGAA GCACCGGTAA CGCCACCCAA ACCTGAGTCG 
CTCTCACCGC CTTTGGAGCC TGCTCTTCCT CCTCCACCTC CTCCAGCCGA AGCAGAGGAT 
GATCCCGCCA TGAAGCCCCA GTCGGTGCTC ACCAGGGTGA AGATGTTCGA GAACAAGCGA 
TCGGTGTCTG TAGACAGAGC CAGAGAGTCT GCAGACTCTG GTATTCGGCC TGTGGACATG 
GTGCCTAAAC CTGGCGCTGC CTCCGTGCCC AAAGCCAATT CTTTAAGTAA CCTGGATCAA 
GAGAAGACCT TTAGAACTCC AGAGCCCCAG CAGCCACAGC CTAAAGTAGA TGATATCATT 
CGTTCAAATA ACAATGACCC TGATGAGGAT GAAGAATATT ACAGGAAACA ACTGTCCTAC 
TTCGACCGCA GGAGCTTCGA CAAACCCTCC ACACAGCTGA CACCTGCCAT CAAACCAGCC 
CAACCCCAAA CTCAACCAGC ACACTATCCC AGAGCAGAGT CAGTGGAGAA AATCAACCCA 
GCTGCTCCAT CAAACCCAGC TGTCCCACCA CCAACCCTGC CTAAACCTGC AGCAACCCCA 
CCTCTCGACC CTCACGGATC TCCCAAAGTA AAGCCTCCTA GTCATGAAGA CACAGTCCAG 
AGCACTTACC TGCCTCACAA AAGCTTCCCA GAGAAATCCC CCGTCAACGG CACCGGCGAA 
CCTCCAAAGA CCAACAGCAC TGCCACGTCC AGCTACAACC GTTTTGTGCC CAAGCCGTAC 
ACCACCTCCG CAAGACCCTT CGAGCGCAAG TTTGACAGTC CCAAGTTCAA TCACAATCTC 
CTCCCTAATG ACTCCCAAAA CAAGACGGAT AATGTGAAAG CTCCCATCAC CGCTAAACCA 
CAGAGCTCAC CCCAGCCGGC AGACCACGAC AGCGGCCTGG ACACCTTCAC ACGCACCATG 
GACCACAAAC CCAAATACCA GCAGAACAAC ATCAACGCCG TACCCAAGGC AATACCTGTC 
AGCCCGAGTG CTTTGGAGGA TGATGACGAT GATGACAGCC ACACGGTGGT GGCGACAGCG 
CGGGGTATTT TCAACTCTAA TGGTGGGGTC CTGAGCTCCA TTGAGACAGG AGTAAGCATT 
ATTATCCCAC AGGGAGCCAT CCCTGATGGC GTGGAGCAAG AGATCTACTT TAAAGTGTGT 
CGGGATAACA GCATCCTGCC ACCTCTGGAC AAAGAGAAAG GAGAAACTCT GTTGAGTCCT 
CTGGTGATGT GTGGTCCTCA CGGTCTGAAA TTCCTGAAGC CAGTGGAGCT CCGCTTACCT 
CACTGTGATC CGAAGAGTTG GCAGAACAAA TCTCTTCCCG GAGATCCCAA CTACCTGGTT 
GGAGCCAACT GTGTGTCTGT GCTTATTGAC CATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tjp1a
Entrez Gene ID	378845
Full Name	tight junction protein 1a
Synonyms	cb817,tjp1
General protein information	Preferred Names tight junction protein 1a Names tight junction protein 1a tight junction protein ZO-1 tight junction protein 1 (zona occludens 1) tjp1.1 zo-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	TJP1	NP_003248.3
	TJP1	XP_002804738.1
Canis lupus familiaris (dog)	TJP1	NP_001003140.1
Rattus norvegicus (Norway rat)	Tjp1	NP_001099736.1
Danio rerio (zebrafish)	LOC565459	XP_003201509.2
Bos taurus (cattle)	TJP1	XP_002696696.3
Gallus gallus (chicken)	TJP1	XP_413773.4
Danio rerio (zebrafish)	tjp1a	XP_005168990.1
Pan troglodytes (chimpanzee)	TJP1	XP_001163120.1
Mus musculus (house mouse)	Tjp1	NP_033412.2
Xenopus tropicalis (tropical clawed frog)	tjp1	XP_004917990.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tjp1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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