alg1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	alg1
Entrez Gene ID	334161
Full Name	ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase
Synonyms	wu:fi34b12,zgc:66221
General protein information	Preferred Names ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase Names chitobiosyldiphosphodolichol beta-mannosyltransferase asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199867.1	NP_956161.1	chitobiosyldiphosphodolichol beta-mannosyltransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALG1	XP_851486.1
Bos taurus (cattle)	ALG1	NP_001192532.1
Caenorhabditis elegans (roundworm)	T26A5.4	NP_498420.2
Arabidopsis thaliana (thale cress)	AT1G16570	NP_173105.1
Pan troglodytes (chimpanzee)	ALG1	XP_510796.2
Saccharomyces cerevisiae (baker's yeast)	ALG1	NP_009668.3
Neurospora crassa	NCU07261	XP_962115.1
Homo sapiens (human)	ALG1	NP_061982.3
Mus musculus (house mouse)	Alg1	NP_663337.2
Kluyveromyces lactis	KLLA0B09405g	XP_451947.1
Eremothecium gossypii	AGOS_ADL338C	NP_983757.2
Schizosaccharomyces pombe (fission yeast)	alg1	NP_593186.1
Oryza sativa (rice)	Os03g0180700	NP_001049165.1
Danio rerio (zebrafish)	alg1	NP_956161.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003551	XP_313298.3
Magnaporthe oryzae (rice blast fungus)	MGG_10494	XP_003713398.1
	LOC710713	XP_001099380.2
Gallus gallus (chicken)	ALG1	XP_414707.3
Drosophila melanogaster (fruit fly)	CG18012	NP_650662.1
Xenopus tropicalis (tropical clawed frog)	alg1	NP_001025606.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans.
Longman D, Hug N, Keith M, Anastasaki C, Patton EE, Grimes G, C?ceres JF
Nucleic acids research41(17)8319-31(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03786	NM_199867.1 Latest version!	Danio rerio ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (alg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alg1 ( NM_199867.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956161.1 Danio rerio ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (alg1), mRNA.

Price & Availability↑

CloneID	ODa03786
Clone ID Related Accession (Same CDS sequence)	NM_199867.1
Accession Version	NM_199867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055554.1. On Jul 6, 2005 this sequence version replaced XM_697055.1. ##Evidence-Data-START## Transcript exon combination :: BC055554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATG CCAATGCGGC AGTAGCTGTA GTGACAGTTT CATTGGTTCT TTTAGGACTT 
TTATCGGGGC TAAGCGTTTT GTGGTCTCTT CTGCCGGTCG CTGTAGTCGT GCTTATTTTT 
GTACTAGCCA GTGGACTAAA GGGACGAGAT GAACTTGCCC ATTTGAACGT TTGCGTGCTG 
GTGCTGGGAG ATATCGGGCG CAGTCCTCGC ATGCAGTATC ATGCGCTCTC TCTCAGCAAA 
CATGGATACA ATGTCACCAT AATTGGCTTT CTCGGAACTA AACCTCATCA AGACATTCTT 
GAAGATGACA GAATAGACAT TCTTCCCATT TCAGAACTAA AGGGGCTAAC AGTTGGCCCC 
AGAATTTTCA GGTATATTTC AAAGGTGACA CTCCAGTCTT TCCAGCTGCT CTATGTCTTG 
ATGACGATTG AAGACCAAGG CTATATTCTT ATGCAGAACC CTCCTGGACT GCCGGCTATA 
GCGGTGACAT GGATGGCGAG CCGTATCAGG GGAAACCAGT TTATCATAGA CTGGCACAAC 
TATGGATACA CCATAATGGC TCTGAGTCAT GGGGAAAATC ATTTAATTGT TAGGTTGGCA 
AAATGGTACG AGAAGATATT TGGATGTCTC TCGGATCACA ACTTGTGTGT CACTAGTGCA 
ATGAGGGAAG ATCTTTGCAA GAACTGGAAT ATTGAGGCAA CCACGTTATA TGACAAGCCG 
CCCTCAATAT TTCGAGAAAC ACCTTTGAAA CTTCAGCATG AGCTGTTTGT CAGAATGGGC 
AGTGCTTATC TACCATTTAG GCCTAGTTCT GCTGTCACTA AGGAGTACAT GGAACTGACA 
GCCTTCACAG AGCGTAACAC TCAGACAGGA GCCGTGACAC GCTCTGCTGG ACGACCTGCT 
CTGCTGATCA GCAGCACCAG CTGGACTGAG GATGAAGACT TCTCAGTTCT GTTACAAGCA 
CTTGAAGAGT ATGAGAAATT TGTTGAGACC GAGAACAGAC TCCCATCGCT GGTTTGTGTG 
ATTACAGGGA AAGGTCCTCA GAAGGAGTAT TACAAGAAAC TGATTGACTC TAGAGAATTT 
CATCATGTCA AGATCTGCAC TCCATGGCTG GAAGCTGAAG ATTATCCTGT TTTACTTGGT 
TCTGCTGATC TTGGGGTCTG CTTGCACAAA TCCTCCAGTG GTCTTGACCT TCCAATGAAA 
GTGGTCGACA TGTTTGGATG TTGCCTGCCA GTCTGTGCCA TACACTTTGA GTGCTTGCAT 
GAGTTGGTGA AACATGAGGA AAATGGGCTC ATTTTCAAAG ACTCCAGTGA GCTGGCTGAA 
CAATTAAAGC TTCTGTTTTT AGATTTCCCT GGTGATCAGG GGAAACTGGG CATCTTCAGG 
AAGAATCTAC AGGAAAGCAG GCAGCAGCGA TGGGATGAGA ACTGGGACCA AAACGTTCTG 
CCACTTATTA AAGAGCACTG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956161.1
CDS	18..1484
Translation	MADANAAVAVVTVSLVLLGLLSGLSVLWSLLPVAVVVLIFVLASGLKGRDELAHLNVCVLVLGDIGRSPRMQYHALSLSKHGYNVTIIGFLGTKPHQDILEDDRIDILPISELKGLTVGPRIFRYISKVTLQSFQLLYVLMTIEDQGYILMQNPPGLPAIAVTWMASRIRGNQFIIDWHNYGYTIMALSHGENHLIVRLAKWYEKIFGCLSDHNLCVTSAMREDLCKNWNIEATTLYDKPPSIFRETPLKLQHELFVRMGSAYLPFRPSSAVTKEYMELTAFTERNTQTGAVTRSAGRPALLISSTSWTEDEDFSVLLQALEEYEKFVETENRLPSLVCVITGKGPQKEYYKKLIDSREFHHVKICTPWLEAEDYPVLLGSADLGVCLHKSSSGLDLPMKVVDMFGCCLPVCAIHFECLHELVKHEENGLIFKDSSELAEQLKLLFLDFPGDQGKLGIFRKNLQESRQQRWDENWDQNVLPLIKEHCD

Target ORF information:

RefSeq Version	NM_199867.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (alg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199867.1

ORF Insert Sequence:

ATGGCGGATG CCAATGCGGC AGTAGCTGTA GTGACAGTTT CATTGGTTCT TTTAGGACTT 
TTATCGGGGC TAAGCGTTTT GTGGTCTCTT CTGCCGGTCG CTGTAGTCGT GCTTATTTTT 
GTACTAGCCA GTGGACTAAA GGGACGAGAT GAACTTGCCC ATTTGAACGT TTGCGTGCTG 
GTGCTGGGAG ATATCGGGCG CAGTCCTCGC ATGCAGTATC ATGCGCTCTC TCTCAGCAAA 
CATGGATACA ATGTCACCAT AATTGGCTTT CTCGGAACTA AACCTCATCA AGACATTCTT 
GAAGATGACA GAATAGACAT TCTTCCCATT TCAGAACTAA AGGGGCTAAC AGTTGGCCCC 
AGAATTTTCA GGTATATTTC AAAGGTGACA CTCCAGTCTT TCCAGCTGCT CTATGTCTTG 
ATGACGATTG AAGACCAAGG CTATATTCTT ATGCAGAACC CTCCTGGACT GCCGGCTATA 
GCGGTGACAT GGATGGCGAG CCGTATCAGG GGAAACCAGT TTATCATAGA CTGGCACAAC 
TATGGATACA CCATAATGGC TCTGAGTCAT GGGGAAAATC ATTTAATTGT TAGGTTGGCA 
AAATGGTACG AGAAGATATT TGGATGTCTC TCGGATCACA ACTTGTGTGT CACTAGTGCA 
ATGAGGGAAG ATCTTTGCAA GAACTGGAAT ATTGAGGCAA CCACGTTATA TGACAAGCCG 
CCCTCAATAT TTCGAGAAAC ACCTTTGAAA CTTCAGCATG AGCTGTTTGT CAGAATGGGC 
AGTGCTTATC TACCATTTAG GCCTAGTTCT GCTGTCACTA AGGAGTACAT GGAACTGACA 
GCCTTCACAG AGCGTAACAC TCAGACAGGA GCCGTGACAC GCTCTGCTGG ACGACCTGCT 
CTGCTGATCA GCAGCACCAG CTGGACTGAG GATGAAGACT TCTCAGTTCT GTTACAAGCA 
CTTGAAGAGT ATGAGAAATT TGTTGAGACC GAGAACAGAC TCCCATCGCT GGTTTGTGTG 
ATTACAGGGA AAGGTCCTCA GAAGGAGTAT TACAAGAAAC TGATTGACTC TAGAGAATTT 
CATCATGTCA AGATCTGCAC TCCATGGCTG GAAGCTGAAG ATTATCCTGT TTTACTTGGT 
TCTGCTGATC TTGGGGTCTG CTTGCACAAA TCCTCCAGTG GTCTTGACCT TCCAATGAAA 
GTGGTCGACA TGTTTGGATG TTGCCTGCCA GTCTGTGCCA TACACTTTGA GTGCTTGCAT 
GAGTTGGTGA AACATGAGGA AAATGGGCTC ATTTTCAAAG ACTCCAGTGA GCTGGCTGAA 
CAATTAAAGC TTCTGTTTTT AGATTTCCCT GGTGATCAGG GGAAACTGGG CATCTTCAGG 
AAGAATCTAC AGGAAAGCAG GCAGCAGCGA TGGGATGAGA ACTGGGACCA AAACGTTCTG 
CCACTTATTA AAGAGCACTG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans.
Nucleic acids research41(17)8319-31(2013 Sep)
Longman D,Hug N,Keith M,Anastasaki C,Patton EE,Grimes G,C?ceres JF