ALG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALG1
Entrez Gene ID	56052
Full Name	ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase
Synonyms	CDG1K,HMAT1,HMT-1,HMT1,MT-1,Mat-1,hMat-1
General protein information	Preferred Names ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase Names chitobiosyldiphosphodolichol beta-mannosyltransferase GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase GDP-mannose-dolichol diphosphochitobiose mannosyltransferase asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog asparagine-linked glycosylation protein 1 homolog beta-1,4-mannosyltransferase mannosyltransferase-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik. [provided by RefSeq, Dec 2008].
Disorder MIM:	605907
Disorder Html:	Congenital disorder of glycosylation, type Ik, 608540 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017023458.1	XP_016878947.1	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform X2
NM_001330504.1	NP_001317433.1	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform 2
NM_019109.5	NP_061982.3	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform 1
NM_019109.4	NP_061982.3	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform 1
XM_017023457.1	XP_016878946.1	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform X1
XM_017023457.2	XP_016878946.1	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALG1	XP_851486.1
Bos taurus (cattle)	ALG1	NP_001192532.1
Caenorhabditis elegans (roundworm)	T26A5.4	NP_498420.2
Arabidopsis thaliana (thale cress)	AT1G16570	NP_173105.1
Pan troglodytes (chimpanzee)	ALG1	XP_510796.2
Saccharomyces cerevisiae (baker's yeast)	ALG1	NP_009668.3
Neurospora crassa	NCU07261	XP_962115.1
Homo sapiens (human)	ALG1	NP_061982.3
Mus musculus (house mouse)	Alg1	NP_663337.2
Kluyveromyces lactis	KLLA0B09405g	XP_451947.1
Eremothecium gossypii	AGOS_ADL338C	NP_983757.2
Schizosaccharomyces pombe (fission yeast)	alg1	NP_593186.1
Oryza sativa (rice)	Os03g0180700	NP_001049165.1
Danio rerio (zebrafish)	alg1	NP_956161.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003551	XP_313298.3
Magnaporthe oryzae (rice blast fungus)	MGG_10494	XP_003713398.1
	LOC710713	XP_001099380.2
Gallus gallus (chicken)	ALG1	XP_414707.3
Drosophila melanogaster (fruit fly)	CG18012	NP_650662.1
Xenopus tropicalis (tropical clawed frog)	alg1	NP_001025606.1

Gene Ontology
Bibliography

Related articles in PubMed

Association studies of WD repeat domain 3 and chitobiosyldiphosphodolichol beta-mannosyltransferase genes with schizophrenia in a Japanese population.
Kobayashi M, Jitoku D, Iwayama Y, Yamamoto N, Toyota T, Suzuki K, Kikuchi M, Hashimoto T, Kanahara N, Kurumaji A, Yoshikawa T, Nishikawa T
PloS one13(1)e0190991(2018)

ALG1-CDG: Clinical and Molecular Characterization of 39 Unreported Patients.
Ng BG, Shiryaev SA, Rymen D, Eklund EA, Raymond K, Kircher M, Abdenur JE, Alehan F, Midro AT, Bamshad MJ, Barone R, Berry GT, Brumbaugh JE, Buckingham KJ, Clarkson K, Cole FS, O'Connor S, Cooper GM, Van Coster R, Demmer LA, Diogo L, Fay AJ, Ficicioglu C, Fiumara A, Gahl WA, Ganetzky R, Goel H, Harshman LA, He M, Jaeken J, James PM, Katz D, Keldermans L, Kibaek M, Kornberg AJ, Lachlan K, Lam C, Yaplito-Lee J, Nickerson DA, Peters HL, Race V, R?gal L, Rush JS, Rutledge SL, Shendure J, Souche E, Sparks SE, Trapane P, Sanchez-Valle A, Vilain E, V?llo A, Waechter CJ, Wang RY, Wolfe LA, Wong DA, Wood T, Yang AC, , Matthijs G, Freeze HH
Human mutation37(7)653-60(2016 07)

Clinical utility gene card for: ALG1 defective congenital disorder of glycosylation.
Jaeken J, Lefeber D, Matthijs G
European journal of human genetics : EJHG23(10)(2015 Oct)

ALG1-CDG: a new case with early fatal outcome.
Rohlfing AK, Rust S, Reunert J, Tirre M, Du Chesne I, Wemhoff S, Meinhardt F, Hartmann H, Das AM, Marquardt T
Gene534(2)345-51(2014 Jan)

Defining the phenotype in congenital disorder of glycosylation due to ALG1 mutations.
Morava E, Vodopiutz J, Lefeber DJ, Janecke AR, Schmidt WM, Lechner S, Item CB, Sykut-Cegielska J, Adamowicz M, Wierzba J, Zhang ZH, Mihalek I, Stockler S, Bodamer OA, Lehle L, Wevers RA
Pediatrics130(4)e1034-9(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study presents molecular, clinical and biochemical findings in the largest collection of ALG1-CDG cases ever reported at a single time with 39 cases, bringing the total number to 57. This ranks it the third most common CDG type behind PMM2-CDG and ALG6-CDG. In addition, highly lethal genotype were identified and confirm the presence of a unique xeno-tetrasaccharide in ALG1-CDG patients.
Title: ALG1-CDG: Clinical and Molecular Characterization of 39 Unreported Patients.

In title.
Title: Clinical utility gene card for: ALG1 defective congenital disorder of glycosylation.

Was detected in the patient's ALG1-coding sequence.
Title: ALG1-CDG: a new case with early fatal outcome.

Family study defining the phenotype of deficiency of beta-1,4 mannosyltransferase (MT-1) congenital disorder of glycosylation (CDG), due to ALG1 gene mutations. Four novel ALG1 mutations were identified.
Title: Defining the phenotype in congenital disorder of glycosylation due to ALG1 mutations.

DNA sequencing of ALG1 revealed nine different mutations, seven of which have not been previously reported. Clinical presentations of deficiency are severe, with dysmorphias, CNS involvement and ocular disturbances
Title: Guanosine diphosphate-mannose:GlcNAc2-PP-dolichol mannosyltransferase deficiency (congenital disorders of glycosylation type Ik): five new patients and seven novel mutations.

The following ALG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu69932	NM_001330504.1 Latest version!	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69932	XM_017023458.1 Latest version!	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu18940	NM_019109.5 Latest version!	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu86152	XM_017023457.2 Latest version!	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu18940	NM_019109.4	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu86152	XM_017023457.1	Homo sapiens ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALG1 ( NM_019109.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061982.3 Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18940
Clone ID Related Accession (Same CDS sequence)	NM_019109.5 , NM_019109.4
Accession Version	NM_019109.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA879477.1, AB019038.1, AC026458.8 and AA810665.1. This sequence is a reference standard in the RefSeqGene project. On Oct 28, 2008 this sequence version replaced NM_019109.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85408.1, SRR1803613.270957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGGACGAA GACTTCTCCA TCCTGCTGGC AGCTTTAGAA 
AAGTTTGAAC AACTGACTCT TGATGGACAC AACCTTCCTT CTCTCGTCTG TGTGATAACA 
GGCAAAGGGC CTCTGAGGGA GTATTATAGC CGCCTCATCC ACCAGAAGCA CTTCCAGCAC 
ATCCAGGTCT GCACCCCCTG GCTGGAGGCC GAGGACTACC CCCTGCTTCT AGGGTCGGCG 
GACCTGGGTG TCTGTCTGCA CACGTCCTCC AGTGGCCTGG ACCTGCCCAT GAAGGTGGTG 
GACATGTTCG GGTGCTGTTT GCCTGTGTGT GCTGTGAACT TCAAGTGTTT ACATGAGCTG 
GTGAAACATG AAGAAAATGG CCTGGTCTTT GAGGACTCAG AGGAACTGGC AGCTCAGCTG 
CAGATGCTTT TCTCAAACTT TCCTGATCCT GCGGGCAAGC TAAACCAGTT CCGGAAGAAC 
CTGCGGGAGT CGCAGCAGCT CCGATGGGAT GAGAGCTGGG TGCAGACTGT GCTCCCTTTG 
GTTATGGACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061982.3
CDS	42..1436
Translation	MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Target ORF information:

RefSeq Version	NM_019109.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019109.4

ORF Insert Sequence:

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGGACGAA GACTTCTCCA TCCTGCTGGC AGCTTTAGAA 
AAGTTTGAAC AACTGACTCT TGATGGACAC AACCTTCCTT CTCTCGTCTG TGTGATAACA 
GGCAAAGGGC CTCTGAGGGA GTATTATAGC CGCCTCATCC ACCAGAAGCA CTTCCAGCAC 
ATCCAGGTCT GCACCCCCTG GCTGGAGGCC GAGGACTACC CCCTGCTTCT AGGGTCGGCG 
GACCTGGGTG TCTGTCTGCA CACGTCCTCC AGTGGCCTGG ACCTGCCCAT GAAGGTGGTG 
GACATGTTCG GGTGCTGTTT GCCTGTGTGT GCTGTGAACT TCAAGTGTTT ACATGAGCTG 
GTGAAACATG AAGAAAATGG CCTGGTCTTT GAGGACTCAG AGGAACTGGC AGCTCAGCTG 
CAGATGCTTT TCTCAAACTT TCCTGATCCT GCGGGCAAGC TAAACCAGTT CCGGAAGAAC 
CTGCGGGAGT CGCAGCAGCT CCGATGGGAT GAGAGCTGGG TGCAGACTGT GCTCCCTTTG 
GTTATGGACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG1 ( NM_001330504.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317433.1 Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu69932
Clone ID Related Accession (Same CDS sequence)	XM_017023458.1 , NM_001330504.1
Accession Version	NM_001330504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Homo sapiens(human)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298144.1, BX370817.2, AC026458.8 and AA810665.1. On Aug 26, 2016 this sequence version replaced XM_011522565.1. Transcript Variant: This variant (2) uses an alternate 5' terminal exon, resulting in a distinct 5' UTR and the use of a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298144.1, SRR1660805.258716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACTTGC TGTGGAAGTT GATGTGGAGG GAGCCAGGTG CCTATATCTT TCTCCAGAAC 
CCCCCAGGTC TGCCTAGCAT TGCTGTCTGC TGGTTCGTGG GCTGCCTTTG TGGAAGCAAG 
CTCGTCATTG ACTGGCACAA CTATGGCTAC TCCATCATGG GTCTGGTGCA TGGCCCCAAC 
CATCCCCTCG TTCTGCTGGC CAAGTGGTAC GAGAAGTTCT TTGGGCGCCT GTCCCACCTG 
AACCTGTGTG TTACCAATGC TATGCGAGAA GACCTGGCGG ATAACTGGCA CATCAGGGCT 
GTGACCGTCT ACGACAAGCC CGCATCTTTC TTTAAAGAGA CACCTCTGGA CCTGCAGCAC 
CGGCTCTTCA TGAAGCTGGG CAGCATGCAC TCTCCGTTCA GGGCCCGCTC AGAACCTGAG 
GACCCAGTCA CGGAGCGGTC GGCCTTCACG GAGCGGGATG CTGGGAGCGG GCTGGTGACG 
CGTCTCCGTG AGCGGCCAGC CCTGCTGGTC AGCAGCACGA GCTGGACAGA GGACGAAGAC 
TTCTCCATCC TGCTGGCAGC TTTAGAAAAG TTTGAACAAC TGACTCTTGA TGGACACAAC 
CTTCCTTCTC TCGTCTGTGT GATAACAGGC AAAGGGCCTC TGAGGGAGTA TTATAGCCGC 
CTCATCCACC AGAAGCACTT CCAGCACATC CAGGTCTGCA CCCCCTGGCT GGAGGCCGAG 
GACTACCCCC TGCTTCTAGG GTCGGCGGAC CTGGGTGTCT GTCTGCACAC GTCCTCCAGT 
GGCCTGGACC TGCCCATGAA GGTGGTGGAC ATGTTCGGGT GCTGTTTGCC TGTGTGTGCT 
GTGAACTTCA AGTGTTTACA TGAGCTGGTG AAACATGAAG AAAATGGCCT GGTCTTTGAG 
GACTCAGAGG AACTGGCAGC TCAGCTGCAG ATGCTTTTCT CAAACTTTCC TGATCCTGCG 
GGCAAGCTAA ACCAGTTCCG GAAGAACCTG CGGGAGTCGC AGCAGCTCCG ATGGGATGAG 
AGCTGGGTGC AGACTGTGCT CCCTTTGGTT ATGGACACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317433.1
CDS	182..1243
Translation	MYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Target ORF information:

RefSeq Version	NM_001330504.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330504.1

ORF Insert Sequence:

ATGTACTTGC TGTGGAAGTT GATGTGGAGG GAGCCAGGTG CCTATATCTT TCTCCAGAAC 
CCCCCAGGTC TGCCTAGCAT TGCTGTCTGC TGGTTCGTGG GCTGCCTTTG TGGAAGCAAG 
CTCGTCATTG ACTGGCACAA CTATGGCTAC TCCATCATGG GTCTGGTGCA TGGCCCCAAC 
CATCCCCTCG TTCTGCTGGC CAAGTGGTAC GAGAAGTTCT TTGGGCGCCT GTCCCACCTG 
AACCTGTGTG TTACCAATGC TATGCGAGAA GACCTGGCGG ATAACTGGCA CATCAGGGCT 
GTGACCGTCT ACGACAAGCC CGCATCTTTC TTTAAAGAGA CACCTCTGGA CCTGCAGCAC 
CGGCTCTTCA TGAAGCTGGG CAGCATGCAC TCTCCGTTCA GGGCCCGCTC AGAACCTGAG 
GACCCAGTCA CGGAGCGGTC GGCCTTCACG GAGCGGGATG CTGGGAGCGG GCTGGTGACG 
CGTCTCCGTG AGCGGCCAGC CCTGCTGGTC AGCAGCACGA GCTGGACAGA GGACGAAGAC 
TTCTCCATCC TGCTGGCAGC TTTAGAAAAG TTTGAACAAC TGACTCTTGA TGGACACAAC 
CTTCCTTCTC TCGTCTGTGT GATAACAGGC AAAGGGCCTC TGAGGGAGTA TTATAGCCGC 
CTCATCCACC AGAAGCACTT CCAGCACATC CAGGTCTGCA CCCCCTGGCT GGAGGCCGAG 
GACTACCCCC TGCTTCTAGG GTCGGCGGAC CTGGGTGTCT GTCTGCACAC GTCCTCCAGT 
GGCCTGGACC TGCCCATGAA GGTGGTGGAC ATGTTCGGGT GCTGTTTGCC TGTGTGTGCT 
GTGAACTTCA AGTGTTTACA TGAGCTGGTG AAACATGAAG AAAATGGCCT GGTCTTTGAG 
GACTCAGAGG AACTGGCAGC TCAGCTGCAG ATGCTTTTCT CAAACTTTCC TGATCCTGCG 
GGCAAGCTAA ACCAGTTCCG GAAGAACCTG CGGGAGTCGC AGCAGCTCCG ATGGGATGAG 
AGCTGGGTGC AGACTGTGCT CCCTTTGGTT ATGGACACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG1 ( XM_017023458.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878947.1 Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu69932
Clone ID Related Accession (Same CDS sequence)	XM_017023458.1 , NM_001330504.1
Accession Version	XM_017023458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGC TGTGGAAGTT GATGTGGAGG GAGCCAGGTG CCTATATCTT TCTCCAGAAC 
CCCCCAGGTC TGCCTAGCAT TGCTGTCTGC TGGTTCGTGG GCTGCCTTTG TGGAAGCAAG 
CTCGTCATTG ACTGGCACAA CTATGGCTAC TCCATCATGG GTCTGGTGCA TGGCCCCAAC 
CATCCCCTCG TTCTGCTGGC CAAGTGGTAC GAGAAGTTCT TTGGGCGCCT GTCCCACCTG 
AACCTGTGTG TTACCAATGC TATGCGAGAA GACCTGGCGG ATAACTGGCA CATCAGGGCT 
GTGACCGTCT ACGACAAGCC CGCATCTTTC TTTAAAGAGA CACCTCTGGA CCTGCAGCAC 
CGGCTCTTCA TGAAGCTGGG CAGCATGCAC TCTCCGTTCA GGGCCCGCTC AGAACCTGAG 
GACCCAGTCA CGGAGCGGTC GGCCTTCACG GAGCGGGATG CTGGGAGCGG GCTGGTGACG 
CGTCTCCGTG AGCGGCCAGC CCTGCTGGTC AGCAGCACGA GCTGGACAGA GGACGAAGAC 
TTCTCCATCC TGCTGGCAGC TTTAGAAAAG TTTGAACAAC TGACTCTTGA TGGACACAAC 
CTTCCTTCTC TCGTCTGTGT GATAACAGGC AAAGGGCCTC TGAGGGAGTA TTATAGCCGC 
CTCATCCACC AGAAGCACTT CCAGCACATC CAGGTCTGCA CCCCCTGGCT GGAGGCCGAG 
GACTACCCCC TGCTTCTAGG GTCGGCGGAC CTGGGTGTCT GTCTGCACAC GTCCTCCAGT 
GGCCTGGACC TGCCCATGAA GGTGGTGGAC ATGTTCGGGT GCTGTTTGCC TGTGTGTGCT 
GTGAACTTCA AGTGTTTACA TGAGCTGGTG AAACATGAAG AAAATGGCCT GGTCTTTGAG 
GACTCAGAGG AACTGGCAGC TCAGCTGCAG ATGCTTTTCT CAAACTTTCC TGATCCTGCG 
GGCAAGCTAA ACCAGTTCCG GAAGAACCTG CGGGAGTCGC AGCAGCTCCG ATGGGATGAG 
AGCTGGGTGC AGACTGTGCT CCCTTTGGTT ATGGACACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878947.1
CDS	497..1558
Translation	MYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Target ORF information:

RefSeq Version	XM_017023458.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023458.1

ORF Insert Sequence:

ATGTACTTGC TGTGGAAGTT GATGTGGAGG GAGCCAGGTG CCTATATCTT TCTCCAGAAC 
CCCCCAGGTC TGCCTAGCAT TGCTGTCTGC TGGTTCGTGG GCTGCCTTTG TGGAAGCAAG 
CTCGTCATTG ACTGGCACAA CTATGGCTAC TCCATCATGG GTCTGGTGCA TGGCCCCAAC 
CATCCCCTCG TTCTGCTGGC CAAGTGGTAC GAGAAGTTCT TTGGGCGCCT GTCCCACCTG 
AACCTGTGTG TTACCAATGC TATGCGAGAA GACCTGGCGG ATAACTGGCA CATCAGGGCT 
GTGACCGTCT ACGACAAGCC CGCATCTTTC TTTAAAGAGA CACCTCTGGA CCTGCAGCAC 
CGGCTCTTCA TGAAGCTGGG CAGCATGCAC TCTCCGTTCA GGGCCCGCTC AGAACCTGAG 
GACCCAGTCA CGGAGCGGTC GGCCTTCACG GAGCGGGATG CTGGGAGCGG GCTGGTGACG 
CGTCTCCGTG AGCGGCCAGC CCTGCTGGTC AGCAGCACGA GCTGGACAGA GGACGAAGAC 
TTCTCCATCC TGCTGGCAGC TTTAGAAAAG TTTGAACAAC TGACTCTTGA TGGACACAAC 
CTTCCTTCTC TCGTCTGTGT GATAACAGGC AAAGGGCCTC TGAGGGAGTA TTATAGCCGC 
CTCATCCACC AGAAGCACTT CCAGCACATC CAGGTCTGCA CCCCCTGGCT GGAGGCCGAG 
GACTACCCCC TGCTTCTAGG GTCGGCGGAC CTGGGTGTCT GTCTGCACAC GTCCTCCAGT 
GGCCTGGACC TGCCCATGAA GGTGGTGGAC ATGTTCGGGT GCTGTTTGCC TGTGTGTGCT 
GTGAACTTCA AGTGTTTACA TGAGCTGGTG AAACATGAAG AAAATGGCCT GGTCTTTGAG 
GACTCAGAGG AACTGGCAGC TCAGCTGCAG ATGCTTTTCT CAAACTTTCC TGATCCTGCG 
GGCAAGCTAA ACCAGTTCCG GAAGAACCTG CGGGAGTCGC AGCAGCTCCG ATGGGATGAG 
AGCTGGGTGC AGACTGTGCT CCCTTTGGTT ATGGACACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG1 ( NM_019109.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061982.3 Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 1, mRNA.

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CloneID	OHu18940
Clone ID Related Accession (Same CDS sequence)	NM_019109.5 , NM_019109.4
Accession Version	NM_019109.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026458.8. On Aug 15, 2019 this sequence version replaced NM_019109.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85408.1, SRR1660809.32966.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGGACGAA GACTTCTCCA TCCTGCTGGC AGCTTTAGAA 
AAGTTTGAAC AACTGACTCT TGATGGACAC AACCTTCCTT CTCTCGTCTG TGTGATAACA 
GGCAAAGGGC CTCTGAGGGA GTATTATAGC CGCCTCATCC ACCAGAAGCA CTTCCAGCAC 
ATCCAGGTCT GCACCCCCTG GCTGGAGGCC GAGGACTACC CCCTGCTTCT AGGGTCGGCG 
GACCTGGGTG TCTGTCTGCA CACGTCCTCC AGTGGCCTGG ACCTGCCCAT GAAGGTGGTG 
GACATGTTCG GGTGCTGTTT GCCTGTGTGT GCTGTGAACT TCAAGTGTTT ACATGAGCTG 
GTGAAACATG AAGAAAATGG CCTGGTCTTT GAGGACTCAG AGGAACTGGC AGCTCAGCTG 
CAGATGCTTT TCTCAAACTT TCCTGATCCT GCGGGCAAGC TAAACCAGTT CCGGAAGAAC 
CTGCGGGAGT CGCAGCAGCT CCGATGGGAT GAGAGCTGGG TGCAGACTGT GCTCCCTTTG 
GTTATGGACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061982.3
CDS	8..1402
Translation	MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Target ORF information:

RefSeq Version	NM_019109.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019109.5

ORF Insert Sequence:

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGGACGAA GACTTCTCCA TCCTGCTGGC AGCTTTAGAA 
AAGTTTGAAC AACTGACTCT TGATGGACAC AACCTTCCTT CTCTCGTCTG TGTGATAACA 
GGCAAAGGGC CTCTGAGGGA GTATTATAGC CGCCTCATCC ACCAGAAGCA CTTCCAGCAC 
ATCCAGGTCT GCACCCCCTG GCTGGAGGCC GAGGACTACC CCCTGCTTCT AGGGTCGGCG 
GACCTGGGTG TCTGTCTGCA CACGTCCTCC AGTGGCCTGG ACCTGCCCAT GAAGGTGGTG 
GACATGTTCG GGTGCTGTTT GCCTGTGTGT GCTGTGAACT TCAAGTGTTT ACATGAGCTG 
GTGAAACATG AAGAAAATGG CCTGGTCTTT GAGGACTCAG AGGAACTGGC AGCTCAGCTG 
CAGATGCTTT TCTCAAACTT TCCTGATCCT GCGGGCAAGC TAAACCAGTT CCGGAAGAAC 
CTGCGGGAGT CGCAGCAGCT CCGATGGGAT GAGAGCTGGG TGCAGACTGT GCTCCCTTTG 
GTTATGGACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG1 ( XM_017023457.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878946.1 Homo sapiens ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X1, mRNA.

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CloneID	OHu86152
Clone ID Related Accession (Same CDS sequence)	XM_017023457.1 , XM_017023457.2
Accession Version	XM_017023457.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGTTTGAA CAACTGACTC TTGATGGACA CAACCTTCCT 
TCTCTCGTCT GTGTGATAAC AGGCAAAGGG CCTCTGAGGG AGTATTATAG CCGCCTCATC 
CACCAGAAGC ACTTCCAGCA CATCCAGGTC TGCACCCCCT GGCTGGAGGC CGAGGACTAC 
CCCCTGCTTC TAGGGTCGGC GGACCTGGGT GTCTGTCTGC ACACGTCCTC CAGTGGCCTG 
GACCTGCCCA TGAAGGTGGT GGACATGTTC GGGTGCTGTT TGCCTGTGTG TGCTGTGAAC 
TTCAAGTGTT TACATGAGCT GGTGAAACAT GAAGAAAATG GCCTGGTCTT TGAGGACTCA 
GAGGAACTGG CAGCTCAGCT GCAGATGCTT TTCTCAAACT TTCCTGATCC TGCGGGCAAG 
CTAAACCAGT TCCGGAAGAA CCTGCGGGAG TCGCAGCAGC TCCGATGGGA TGAGAGCTGG 
GTGCAGACTG TGCTCCCTTT GGTTATGGAC ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878946.1
CDS	42..1397
Translation	MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Target ORF information:

RefSeq Version	XM_017023457.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023457.1

ORF Insert Sequence:

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGTTTGAA CAACTGACTC TTGATGGACA CAACCTTCCT 
TCTCTCGTCT GTGTGATAAC AGGCAAAGGG CCTCTGAGGG AGTATTATAG CCGCCTCATC 
CACCAGAAGC ACTTCCAGCA CATCCAGGTC TGCACCCCCT GGCTGGAGGC CGAGGACTAC 
CCCCTGCTTC TAGGGTCGGC GGACCTGGGT GTCTGTCTGC ACACGTCCTC CAGTGGCCTG 
GACCTGCCCA TGAAGGTGGT GGACATGTTC GGGTGCTGTT TGCCTGTGTG TGCTGTGAAC 
TTCAAGTGTT TACATGAGCT GGTGAAACAT GAAGAAAATG GCCTGGTCTT TGAGGACTCA 
GAGGAACTGG CAGCTCAGCT GCAGATGCTT TTCTCAAACT TTCCTGATCC TGCGGGCAAG 
CTAAACCAGT TCCGGAAGAA CCTGCGGGAG TCGCAGCAGC TCCGATGGGA TGAGAGCTGG 
GTGCAGACTG TGCTCCCTTT GGTTATGGAC ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG1 ( XM_017023457.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878946.1 Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86152
Clone ID Related Accession (Same CDS sequence)	XM_017023457.1 , XM_017023457.2
Accession Version	XM_017023457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chitobiosyldiphosphodolichol beta-mannosyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGTTTGAA CAACTGACTC TTGATGGACA CAACCTTCCT 
TCTCTCGTCT GTGTGATAAC AGGCAAAGGG CCTCTGAGGG AGTATTATAG CCGCCTCATC 
CACCAGAAGC ACTTCCAGCA CATCCAGGTC TGCACCCCCT GGCTGGAGGC CGAGGACTAC 
CCCCTGCTTC TAGGGTCGGC GGACCTGGGT GTCTGTCTGC ACACGTCCTC CAGTGGCCTG 
GACCTGCCCA TGAAGGTGGT GGACATGTTC GGGTGCTGTT TGCCTGTGTG TGCTGTGAAC 
TTCAAGTGTT TACATGAGCT GGTGAAACAT GAAGAAAATG GCCTGGTCTT TGAGGACTCA 
GAGGAACTGG CAGCTCAGCT GCAGATGCTT TTCTCAAACT TTCCTGATCC TGCGGGCAAG 
CTAAACCAGT TCCGGAAGAA CCTGCGGGAG TCGCAGCAGC TCCGATGGGA TGAGAGCTGG 
GTGCAGACTG TGCTCCCTTT GGTTATGGAC ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878946.1
CDS	26..1381
Translation	MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Target ORF information:

RefSeq Version	XM_017023457.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023457.2

ORF Insert Sequence:

ATGGCGGCCT CATGCTTGGT CCTGCTGGCG CTGTGTCTGC TGCTGCCGCT GCTGCTGCTG 
GGAGGATGGA AGCGCTGGCG CCGGGGGCGG GCGGCCCGGC ATGTAGTAGC GGTGGTGCTG 
GGCGACGTGG GCCGCAGCCC CCGTATGCAG TACCACGCGC TGTCGTTGGC CATGCACGGC 
TTCTCGGTGA CCCTCCTGGG GTTCTGCAAC TCCAAACCCC ATGATGAGCT CTTGCAGAAC 
AACAGAATTC AGATTGTGGG GTTGACAGAA CTTCAGAGTC TTGCAGTTGG GCCCCGAGTT 
TTCCAGTACG GAGTCAAAGT TGTACTTCAG GCTATGTACT TGCTGTGGAA GTTGATGTGG 
AGGGAGCCAG GTGCCTATAT CTTTCTCCAG AACCCCCCAG GTCTGCCTAG CATTGCTGTC 
TGCTGGTTCG TGGGCTGCCT TTGTGGAAGC AAGCTCGTCA TTGACTGGCA CAACTATGGC 
TACTCCATCA TGGGTCTGGT GCATGGCCCC AACCATCCCC TCGTTCTGCT GGCCAAGTGG 
TACGAGAAGT TCTTTGGGCG CCTGTCCCAC CTGAACCTGT GTGTTACCAA TGCTATGCGA 
GAAGACCTGG CGGATAACTG GCACATCAGG GCTGTGACCG TCTACGACAA GCCCGCATCT 
TTCTTTAAAG AGACACCTCT GGACCTGCAG CACCGGCTCT TCATGAAGCT GGGCAGCATG 
CACTCTCCGT TCAGGGCCCG CTCAGAACCT GAGGACCCAG TCACGGAGCG GTCGGCCTTC 
ACGGAGCGGG ATGCTGGGAG CGGGCTGGTG ACGCGTCTCC GTGAGCGGCC AGCCCTGCTG 
GTCAGCAGCA CGAGCTGGAC AGAGTTTGAA CAACTGACTC TTGATGGACA CAACCTTCCT 
TCTCTCGTCT GTGTGATAAC AGGCAAAGGG CCTCTGAGGG AGTATTATAG CCGCCTCATC 
CACCAGAAGC ACTTCCAGCA CATCCAGGTC TGCACCCCCT GGCTGGAGGC CGAGGACTAC 
CCCCTGCTTC TAGGGTCGGC GGACCTGGGT GTCTGTCTGC ACACGTCCTC CAGTGGCCTG 
GACCTGCCCA TGAAGGTGGT GGACATGTTC GGGTGCTGTT TGCCTGTGTG TGCTGTGAAC 
TTCAAGTGTT TACATGAGCT GGTGAAACAT GAAGAAAATG GCCTGGTCTT TGAGGACTCA 
GAGGAACTGG CAGCTCAGCT GCAGATGCTT TTCTCAAACT TTCCTGATCC TGCGGGCAAG 
CTAAACCAGT TCCGGAAGAA CCTGCGGGAG TCGCAGCAGC TCCGATGGGA TGAGAGCTGG 
GTGCAGACTG TGCTCCCTTT GGTTATGGAC ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association studies of WD repeat domain 3 and chitobiosyldiphosphodolichol beta-mannosyltransferase genes with schizophrenia in a Japanese population.
PloS one13(1)e0190991(2018)
Kobayashi M,Jitoku D,Iwayama Y,Yamamoto N,Toyota T,Suzuki K,Kikuchi M,Hashimoto T,Kanahara N,Kurumaji A,Yoshikawa T,Nishikawa T

ALG1-CDG: Clinical and Molecular Characterization of 39 Unreported Patients.
Human mutation37(7)653-60(2016 07)
Ng BG,Shiryaev SA,Rymen D,Eklund EA,Raymond K,Kircher M,Abdenur JE,Alehan F,Midro AT,Bamshad MJ,Barone R,Berry GT,Brumbaugh JE,Buckingham KJ,Clarkson K,Cole FS,O'Connor S,Cooper GM,Van Coster R,Demmer LA,Diogo L,Fay AJ,Ficicioglu C,Fiumara A,Gahl WA,Ganetzky R,Goel H,Harshman LA,He M,Jaeken J,James PM,Katz D,Keldermans L,Kibaek M,Kornberg AJ,Lachlan K,Lam C,Yaplito-Lee J,Nickerson DA,Peters HL,Race V,R?gal L,Rush JS,Rutledge SL,Shendure J,Souche E,Sparks SE,Trapane P,Sanchez-Valle A,Vilain E,V?llo A,Waechter CJ,Wang RY,Wolfe LA,Wong DA,Wood T,Yang AC,,Matthijs G,Freeze HH

Clinical utility gene card for: ALG1 defective congenital disorder of glycosylation.
European journal of human genetics : EJHG23(10)(2015 Oct)
Jaeken J,Lefeber D,Matthijs G

ALG1-CDG: a new case with early fatal outcome.
Gene534(2)345-51(2014 Jan)
Rohlfing AK,Rust S,Reunert J,Tirre M,Du Chesne I,Wemhoff S,Meinhardt F,Hartmann H,Das AM,Marquardt T

Defining the phenotype in congenital disorder of glycosylation due to ALG1 mutations.
Pediatrics130(4)e1034-9(2012 Oct)
Morava E,Vodopiutz J,Lefeber DJ,Janecke AR,Schmidt WM,Lechner S,Item CB,Sykut-Cegielska J,Adamowicz M,Wierzba J,Zhang ZH,Mihalek I,Stockler S,Bodamer OA,Lehle L,Wevers RA

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ALG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム