anpepb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	anpepb
Entrez Gene ID	322533
Full Name	alanyl (membrane) aminopeptidase b
Synonyms	anpep,fb64c05,wu:fb64c05,zgc:136771
General protein information	Preferred Names alanyl (membrane) aminopeptidase b Names aminopeptidase N
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001089325.1	NP_001082794.1	aminopeptidase N

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	anpepb	NP_001082794.1
Danio rerio (zebrafish)	LOC100535900	XP_003201453.1
Danio rerio (zebrafish)	LOC100535773	XP_003201450.1
	ANPEP	XP_001093727.1
Bos taurus (cattle)	ANPEP	NP_001068612.1
Rattus norvegicus (Norway rat)	Anpep	NP_112274.1
Danio rerio (zebrafish)	LOC555478	XP_682987.1
Danio rerio (zebrafish)	LOC555317	XP_001922987.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013001	XP_003436574.1
Mus musculus (house mouse)	Anpep	NP_032512.2
Danio rerio (zebrafish)	LOC100004744	XP_001920418.2
Drosophila melanogaster (fruit fly)	CG14516	NP_001263055.1
Homo sapiens (human)	ANPEP	NP_001141.2
Pan troglodytes (chimpanzee)	ANPEP	XP_523153.3
Canis lupus familiaris (dog)	ANPEP	NP_001139506.1
Gallus gallus (chicken)	ANPEP	NP_990192.1
Xenopus tropicalis (tropical clawed frog)	anpep	XP_002932303.2

Gene Ontology
Bibliography

Related articles in PubMed

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Li JM, Li LY, Qin X, Ning LJ, Lu DL, Li DL, Zhang ML, Wang X, Du ZY
Scientific reports740815(2017 Jan)

Molecular characterization and dietary regulation of aminopeptidase N (APN) in the grass carp (Ctenopharyngodon idella).
Tang J, Qu F, Tang X, Zhao Q, Wang Y, Zhou Y, Feng J, Lu S, Hou D, Liu Z
Gene582(1)77-84(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following anpepb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the anpepb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33371	NM_001089325.1 Latest version!	Danio rerio alanyl (membrane) aminopeptidase b (anpepb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

anpepb ( NM_001089325.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082794.1 Danio rerio alanyl (membrane) aminopeptidase b (anpepb), mRNA.

Price & Availability↑

CloneID	ODa33371
Clone ID Related Accession (Same CDS sequence)	NM_001089325.1
Accession Version	NM_001089325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Danio rerio(zebrafish)
Product	aminopeptidase N
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC115260.1. ##Evidence-Data-START## Transcript exon combination :: BC115260.1, BC154244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAAGG GTTTCTACAT CAGCAAAACC CTCGGGATAG TAGGGATTGT GATAGGCGCA 
GGAGCGGTGG CCACAATCAT CGCCCTTTCT GTGGTCTATT CCCAAGAGAA GTCCAAAAAC 
AACGAGATCG AGAACCAACA GACGACTACA ACAAAGGGCC CGACAACAAA TGTTCCAGCG 
ACGACAAACA TCCCAACAAC AGTACCATCC AATGAACCAT GGGACCAATG GCGGCTTCCT 
CAAACTCTCA GTCCAGAAAC CTACAAAGTC ACCCTGTGGC CGAGGCTCCA GAAGAACGCT 
GAGGGACTTT ACATCTTCAC GGGAGATTCA AGTGTGGTCT TCAGATGTGT GGAAAACACA 
GATCTGATCC TCATTCACTC CAATAAGCTG ACCATCAAAG ACTCGACCAC TTTAAAAGCA 
CTCGGAGGTA ATCCAGCTCC TAACATTGTG TCAACTAAGA TGTATCCAAA AACCCAATAC 
ATGGCGATTT GGCTGGATCG AGAATTAACT GCTGGGGAAA GTTATGAGCT TTACACTGAA 
TTCGTGGGAG AGCTTTCTGA TGACTTGGGT GGCTTTTACC GGAGTGAATA TTATGATGAG 
AATGGTGTGC TCAAAGTGGT CGCCACAACT CAGATGCAGG CAACGGATGC CAGAAAAGCA 
TTTCCTTGCT TTGATGAACC AGCAATGAAA GCCGTGTTCA ACATCGTTCT TCTCCATGAT 
CCAGGAACTG TCGCGCTCTC CAATGGCGTT GTGATCGAGG AGATTCCAGT CACAGTGGAT 
GGCATTTCTT TAACCAAAAC CACATTCGCA CCCACAGAAA AAATGTCGAC ATATTTGTTG 
GCATTTATAG TCAGCGAATT CACCTACATT GAACAAAAAC TTGATGATCT GCAGATTCGA 
ATTTTTGCAC GTAAAGAAGC CATTGATGCA AATCAAGGAG AGTATGCACT CAGTGTGACC 
GGAAAAATTC TCCGATTCTT TGAGGAGTAT TACAACTCGT CCTACCCACT GCCCAAATCA 
GATCAAATCG CTCTGCCGGA CTTCAACGCT GGTGCAATGG AAAACTGGGG TTTAATCACG 
TACAGAGAGA CAGCCCTGCT GTACGATGAA GAAATGTCCT CAAATGGAAA CAAAGAGAGG 
GTCGTCACTG TTATAGCCCA TGAACTTGCC CATCAGTGGT TCGGAAACCT TGTGACTATC 
AGGTGGTGGA ATGACCTGTG GCTCAATGAG GGTTTTGCCT CTTATGTGGA ATATCTTGGG 
GCGGATGAAG CTGAGCCATT ATGGAACATT AAAGATCTGA TCGTTCTTAA TGATGTGCAC 
CGAGTTTTCG CTATTGATGC GTTGGCCTCA TCTCATCCCT TATCATCTAA AGAAGAGGAT 
GTCCAAAGGC CGGAGCAAAT TAGCGAAGTG TTTGACACAA TCTCCTACAG TAAGGGGGCA 
TCAGTACTCA GGATGCTATC AAACTTTCTG TCTGAGGACG TGTTTACTCA AGGGCTCAGA 
ACCTACTTGG AACATTTCAA ATTTAACAAC ACAGTGTACA CAGATCTTTG GGACCATCTT 
CAAATGGCTG TGGACGAGAC TGGAACTGAA CTCCCCAGAT CTGTGAAAGA TATTATGGAC 
CGCTGGGTTC TTCAAATGGG CTTCCCTGTG GTCACTATTA ACACCGTAAC TGGACAGATC 
TCACAGGAGC ACTTCTTATT GGACCCTGAA ACCAAACCAG AGCCATCAGA ATTCAACTAT 
GAGTGGTTTG TACCAATTAC ATGGACAAAG AATGAAGCAA TAAAACCCCA GTATTGGCTG 
CTGCAGAAAA ACACTCAATT CGATGACATG AAAACGAATG CAAATGAGTG GGTTCTCGCC 
AACATCAACA CCGTAGGATA CTACAGGGTT AACTATGATG AGCAAAACTG GGAACGCCTG 
CTTAATGCTC TCCAGACATC CAGAGAGAGT ATTCCAGTTA TTAACCGGGC ACAGCTAATC 
GATGACGCTT TCAACCTGGC CAAAGCAGGA ATTATTAAAA CAACCTTGGC TCTAAGAACC 
ACAGAGTTTC TAGATGTAGA AACCGAATAC ATGCCATGGC AGTCTGCTCT CAACAACCTG 
GACTACTTTT ACCTCATGTT TGATCGCTCA GAGGTTTACG GCCACATGCA GGCATACATT 
AGAAAGCAGG TGACACCCTT GTTTGAATAC TTTACAGAAC TAACAGACTG GCAGGGTGTG 
CCTGACAATC ATACTGAACA ATACAATCAA GTGAATGCTC TCCGAGTTGC CTGCAGCATT 
GGGTTAAAAA ATTGTACAGA TCTTGTTACA TCATGGTTCG GAGAGTGGAT GAACAATGAA 
GATGTCAACC CTATTCATGC CAACTTGAGG TCTACAGTTT ACTGCAGTGC CATTGCTGCA 
GGTGGTGCTG AAGAGTGGGA ATTCGCCTGG AAAATGTTTG AAAAGACATC CGTCGCTTCA 
GAAAAAGACA AACTGAGAGC TGCAATGGCA TGTGCCACCC AGCCCTGGTT ACTGAACAGG 
TATCTCGAAT ACACTTTGGA TGCAAACAAA ATTCGCAAGC AAGATGCTAC TTCTACCATT 
GTCAGCATTG CAAGTAATGT AGGCGGCCAA TCATTGGCTT GGGATTTTGT TCGAGCCAAT 
TGGGAGTACA TTTTCAACCA GTATGGTGGT GGATCTTTCT CTTTCTCCAA TTTGATCAAC 
GGTGTCACAA AGAGATTCTC CACTGAGTTT GAATTGAAAC AGCTAATGCA GTTCAAGGAG 
GACAACGAGC ACATCGGCTT CGGTTCTGGA TCCCTAGCCA TTCAGCAATC CATTGAGAGG 
ACAAAAGCCA ACATCAAATG GGTGGAACAG AACAAAGCTG ATGTTTTACA GTGGTTTAAA 
GACCAATCCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082794.1
CDS	14..2911
Translation	MGKGFYISKTLGIVGIVIGAGAVATIIALSVVYSQEKSKNNEIENQQTTTTKGPTTNVPATTNIPTTVPSNEPWDQWRLPQTLSPETYKVTLWPRLQKNAEGLYIFTGDSSVVFRCVENTDLILIHSNKLTIKDSTTLKALGGNPAPNIVSTKMYPKTQYMAIWLDRELTAGESYELYTEFVGELSDDLGGFYRSEYYDENGVLKVVATTQMQATDARKAFPCFDEPAMKAVFNIVLLHDPGTVALSNGVVIEEIPVTVDGISLTKTTFAPTEKMSTYLLAFIVSEFTYIEQKLDDLQIRIFARKEAIDANQGEYALSVTGKILRFFEEYYNSSYPLPKSDQIALPDFNAGAMENWGLITYRETALLYDEEMSSNGNKERVVTVIAHELAHQWFGNLVTIRWWNDLWLNEGFASYVEYLGADEAEPLWNIKDLIVLNDVHRVFAIDALASSHPLSSKEEDVQRPEQISEVFDTISYSKGASVLRMLSNFLSEDVFTQGLRTYLEHFKFNNTVYTDLWDHLQMAVDETGTELPRSVKDIMDRWVLQMGFPVVTINTVTGQISQEHFLLDPETKPEPSEFNYEWFVPITWTKNEAIKPQYWLLQKNTQFDDMKTNANEWVLANINTVGYYRVNYDEQNWERLLNALQTSRESIPVINRAQLIDDAFNLAKAGIIKTTLALRTTEFLDVETEYMPWQSALNNLDYFYLMFDRSEVYGHMQAYIRKQVTPLFEYFTELTDWQGVPDNHTEQYNQVNALRVACSIGLKNCTDLVTSWFGEWMNNEDVNPIHANLRSTVYCSAIAAGGAEEWEFAWKMFEKTSVASEKDKLRAAMACATQPWLLNRYLEYTLDANKIRKQDATSTIVSIASNVGGQSLAWDFVRANWEYIFNQYGGGSFSFSNLINGVTKRFSTEFELKQLMQFKEDNEHIGFGSGSLAIQQSIERTKANIKWVEQNKADVLQWFKDQSKP

Target ORF information:

RefSeq Version	NM_001089325.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alanyl (membrane) aminopeptidase b (anpepb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089325.1

ORF Insert Sequence:

ATGGGAAAGG GTTTCTACAT CAGCAAAACC CTCGGGATAG TAGGGATTGT GATAGGCGCA 
GGAGCGGTGG CCACAATCAT CGCCCTTTCT GTGGTCTATT CCCAAGAGAA GTCCAAAAAC 
AACGAGATCG AGAACCAACA GACGACTACA ACAAAGGGCC CGACAACAAA TGTTCCAGCG 
ACGACAAACA TCCCAACAAC AGTACCATCC AATGAACCAT GGGACCAATG GCGGCTTCCT 
CAAACTCTCA GTCCAGAAAC CTACAAAGTC ACCCTGTGGC CGAGGCTCCA GAAGAACGCT 
GAGGGACTTT ACATCTTCAC GGGAGATTCA AGTGTGGTCT TCAGATGTGT GGAAAACACA 
GATCTGATCC TCATTCACTC CAATAAGCTG ACCATCAAAG ACTCGACCAC TTTAAAAGCA 
CTCGGAGGTA ATCCAGCTCC TAACATTGTG TCAACTAAGA TGTATCCAAA AACCCAATAC 
ATGGCGATTT GGCTGGATCG AGAATTAACT GCTGGGGAAA GTTATGAGCT TTACACTGAA 
TTCGTGGGAG AGCTTTCTGA TGACTTGGGT GGCTTTTACC GGAGTGAATA TTATGATGAG 
AATGGTGTGC TCAAAGTGGT CGCCACAACT CAGATGCAGG CAACGGATGC CAGAAAAGCA 
TTTCCTTGCT TTGATGAACC AGCAATGAAA GCCGTGTTCA ACATCGTTCT TCTCCATGAT 
CCAGGAACTG TCGCGCTCTC CAATGGCGTT GTGATCGAGG AGATTCCAGT CACAGTGGAT 
GGCATTTCTT TAACCAAAAC CACATTCGCA CCCACAGAAA AAATGTCGAC ATATTTGTTG 
GCATTTATAG TCAGCGAATT CACCTACATT GAACAAAAAC TTGATGATCT GCAGATTCGA 
ATTTTTGCAC GTAAAGAAGC CATTGATGCA AATCAAGGAG AGTATGCACT CAGTGTGACC 
GGAAAAATTC TCCGATTCTT TGAGGAGTAT TACAACTCGT CCTACCCACT GCCCAAATCA 
GATCAAATCG CTCTGCCGGA CTTCAACGCT GGTGCAATGG AAAACTGGGG TTTAATCACG 
TACAGAGAGA CAGCCCTGCT GTACGATGAA GAAATGTCCT CAAATGGAAA CAAAGAGAGG 
GTCGTCACTG TTATAGCCCA TGAACTTGCC CATCAGTGGT TCGGAAACCT TGTGACTATC 
AGGTGGTGGA ATGACCTGTG GCTCAATGAG GGTTTTGCCT CTTATGTGGA ATATCTTGGG 
GCGGATGAAG CTGAGCCATT ATGGAACATT AAAGATCTGA TCGTTCTTAA TGATGTGCAC 
CGAGTTTTCG CTATTGATGC GTTGGCCTCA TCTCATCCCT TATCATCTAA AGAAGAGGAT 
GTCCAAAGGC CGGAGCAAAT TAGCGAAGTG TTTGACACAA TCTCCTACAG TAAGGGGGCA 
TCAGTACTCA GGATGCTATC AAACTTTCTG TCTGAGGACG TGTTTACTCA AGGGCTCAGA 
ACCTACTTGG AACATTTCAA ATTTAACAAC ACAGTGTACA CAGATCTTTG GGACCATCTT 
CAAATGGCTG TGGACGAGAC TGGAACTGAA CTCCCCAGAT CTGTGAAAGA TATTATGGAC 
CGCTGGGTTC TTCAAATGGG CTTCCCTGTG GTCACTATTA ACACCGTAAC TGGACAGATC 
TCACAGGAGC ACTTCTTATT GGACCCTGAA ACCAAACCAG AGCCATCAGA ATTCAACTAT 
GAGTGGTTTG TACCAATTAC ATGGACAAAG AATGAAGCAA TAAAACCCCA GTATTGGCTG 
CTGCAGAAAA ACACTCAATT CGATGACATG AAAACGAATG CAAATGAGTG GGTTCTCGCC 
AACATCAACA CCGTAGGATA CTACAGGGTT AACTATGATG AGCAAAACTG GGAACGCCTG 
CTTAATGCTC TCCAGACATC CAGAGAGAGT ATTCCAGTTA TTAACCGGGC ACAGCTAATC 
GATGACGCTT TCAACCTGGC CAAAGCAGGA ATTATTAAAA CAACCTTGGC TCTAAGAACC 
ACAGAGTTTC TAGATGTAGA AACCGAATAC ATGCCATGGC AGTCTGCTCT CAACAACCTG 
GACTACTTTT ACCTCATGTT TGATCGCTCA GAGGTTTACG GCCACATGCA GGCATACATT 
AGAAAGCAGG TGACACCCTT GTTTGAATAC TTTACAGAAC TAACAGACTG GCAGGGTGTG 
CCTGACAATC ATACTGAACA ATACAATCAA GTGAATGCTC TCCGAGTTGC CTGCAGCATT 
GGGTTAAAAA ATTGTACAGA TCTTGTTACA TCATGGTTCG GAGAGTGGAT GAACAATGAA 
GATGTCAACC CTATTCATGC CAACTTGAGG TCTACAGTTT ACTGCAGTGC CATTGCTGCA 
GGTGGTGCTG AAGAGTGGGA ATTCGCCTGG AAAATGTTTG AAAAGACATC CGTCGCTTCA 
GAAAAAGACA AACTGAGAGC TGCAATGGCA TGTGCCACCC AGCCCTGGTT ACTGAACAGG 
TATCTCGAAT ACACTTTGGA TGCAAACAAA ATTCGCAAGC AAGATGCTAC TTCTACCATT 
GTCAGCATTG CAAGTAATGT AGGCGGCCAA TCATTGGCTT GGGATTTTGT TCGAGCCAAT 
TGGGAGTACA TTTTCAACCA GTATGGTGGT GGATCTTTCT CTTTCTCCAA TTTGATCAAC 
GGTGTCACAA AGAGATTCTC CACTGAGTTT GAATTGAAAC AGCTAATGCA GTTCAAGGAG 
GACAACGAGC ACATCGGCTT CGGTTCTGGA TCCCTAGCCA TTCAGCAATC CATTGAGAGG 
ACAAAAGCCA ACATCAAATG GGTGGAACAG AACAAAGCTG ATGTTTTACA GTGGTTTAAA 
GACCAATCCA AACCCTAA