b3gat3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	b3gat3
Entrez Gene ID	192334
Full Name	beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
Synonyms	CHUNP6865,fe01c06,wu:fe01c06,zgc:112070
General protein information	Preferred Names beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) Names galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 3-glucuronyltransferase 3 beta3-glucuronyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001015059.2	NP_001015059.2	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B3GAT3	NP_001229555.1
Canis lupus familiaris (dog)	B3GAT3	XP_540906.2
Bos taurus (cattle)	B3GAT3	NP_991374.2
Homo sapiens (human)	B3GAT3	NP_036332.2
Mus musculus (house mouse)	B3gat3	NP_077218.1
Rattus norvegicus (Norway rat)	B3gat3	NP_001121656.1
Danio rerio (zebrafish)	b3gat3	NP_001015059.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002801	XP_312113.5
Drosophila melanogaster (fruit fly)	GlcAT-I	NP_726910.1
Caenorhabditis elegans (roundworm)	sqv-8	NP_496076.1
Xenopus tropicalis (tropical clawed frog)	b3gat3	NP_001025520.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

On the roles and regulation of chondroitin sulfate and heparan sulfate in zebrafish pharyngeal cartilage morphogenesis.
Holmborn K, Habicher J, Kasza Z, Eriksson AS, Filipek-Gorniok B, Gopal S, Couchman JR, Ahlberg PE, Wiweger M, Spillmann D, Kreuger J, Ledin J
The Journal of biological chemistry287(40)33905-16(2012 Sep)

Growth plate regulation and osteochondroma formation: insights from tracing proteoglycans in zebrafish models and human cartilage.
de Andrea CE, Prins FA, Wiweger MI, Hogendoorn PC
The Journal of pathology224(2)160-8(2011 Jun)

Cartilage ultrastructure in proteoglycan-deficient zebrafish mutants brings to light new candidate genes for human skeletal disorders.
Wiweger MI, Avramut CM, de Andrea CE, Prins FA, Koster AJ, Ravelli RB, Hogendoorn PC
The Journal of pathology223(4)531-42(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of chondroitin sulfate and heparan sulfate biosynthesis in zebrafish larvae homozygous for mutations in ext2, extl3, uxs1, or b3gat3
Title: On the roles and regulation of chondroitin sulfate and heparan sulfate in zebrafish pharyngeal cartilage morphogenesis.

The following b3gat3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the b3gat3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53235	NM_001015059.2 Latest version!	Danio rerio beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (b3gat3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

b3gat3 ( NM_001015059.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001015059.2 Danio rerio beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (b3gat3), mRNA.

Price & Availability↑

CloneID	ODa53235
Clone ID Related Accession (Same CDS sequence)	NM_001015059.2
Accession Version	NM_001015059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX547932.17, CU856512.4 and AL954715.13. On Jan 27, 2016 this sequence version replaced NM_001015059.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC093183.1, AF506207.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGGATGA GACTGAAGCT GAAGACAGTG TTTGTCCTGT ACTTCATGGT GTCTCTGTTT 
GGGCTCCTGT ACGCGCTGAT GCAGCTCGGT CAGCGCTGTG ACTGTCGAGA TCATGAGCAA 
TCTAAAGATC AGCAGATCTC TCAGCTGAAA GGGGAACTGC AGAAGCTGCA GGAGCACATC 
AAAACATCAG AGCTGTCCAA GAAGACGGAT GTGCCCAGAA TATACGTCAT AACACCCACA 
TATGCTAGAC TGGTGCAGAA AGCTGAGCTC ACTCGTCTAT CTCACACATT CCTCCACGTT 
CCCCAGCTGC ACTGGATCGT GGTGGAAGAT GCTCCTCAGC AAACTCAGCT CGTCTCCGAC 
TTCCTGTCTG CTTCCGGCTT GACCTACACT CATCTAAACA AGCTGACGCC CAAGGAGAGG 
AAGCTGCAGG AGGGAGACCC CAACTGGCTG AAGCCCCGGG GGGCTGAGCA GAGGAACGAG 
GGTCTGCGCT GGCTCAGATG GATGGGTTCT ACAGTTCACG GGAAAGAAGC AGCTGCCCTT 
GAAGAGGCCG TGGTTTACTT TGCTGATGAT GACAACACGT ACAGCCTACA GCTTTTTGAA 
GAGATGCGCT ATACTTACCG TGTGTCAGTG TGGCCTGTGG GTCTGGTGGG TGGGATGAAG 
TTTGAGCGGC CCGTGGTGGA GGATGGGAAG GTTGTGCGTT TCCACACTGG CTGGCGTCCC 
AATCGGCCCT TCCCTATCGA CATGGCTGGA TTTGCAGTGT CCCTGAGGCT GGTACTGACC 
AATAAGGAAG CTTTGTTTGA CGGAGACGCT CAGATGGGCT TCCTGGAAAG CAGTTTCCTT 
CAGCACCTGG TCACTATGGA TGACCTTGAA CCGAAAGCAG ACCTGTGCAC CAAGGTGCTG 
GTTTGGCACA CCCGGACTGA GAAGCCAAAG ATGAAGAGAG AAGAAGCTTT GCTGAAGCAG 
GGGATGGGCT CTGATCCGGA TGTGGAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015059.2
CDS	226..1218
Translation	MRMRLKLKTVFVLYFMVSLFGLLYALMQLGQRCDCRDHEQSKDQQISQLKGELQKLQEHIKTSELSKKTDVPRIYVITPTYARLVQKAELTRLSHTFLHVPQLHWIVVEDAPQQTQLVSDFLSASGLTYTHLNKLTPKERKLQEGDPNWLKPRGAEQRNEGLRWLRWMGSTVHGKEAAALEEAVVYFADDDNTYSLQLFEEMRYTYRVSVWPVGLVGGMKFERPVVEDGKVVRFHTGWRPNRPFPIDMAGFAVSLRLVLTNKEALFDGDAQMGFLESSFLQHLVTMDDLEPKADLCTKVLVWHTRTEKPKMKREEALLKQGMGSDPDVEV

Target ORF information:

RefSeq Version	NM_001015059.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (b3gat3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015059.2

ORF Insert Sequence:

ATGAGGATGA GACTGAAGCT GAAGACAGTG TTTGTCCTGT ACTTCATGGT GTCTCTGTTT 
GGGCTCCTGT ACGCGCTGAT GCAGCTCGGT CAGCGCTGTG ACTGTCGAGA TCATGAGCAA 
TCTAAAGATC AGCAGATCTC TCAGCTGAAA GGGGAACTGC AGAAGCTGCA GGAGCACATC 
AAAACATCAG AGCTGTCCAA GAAGACGGAT GTGCCCAGAA TATACGTCAT AACACCCACA 
TATGCTAGAC TGGTGCAGAA AGCTGAGCTC ACTCGTCTAT CTCACACATT CCTCCACGTT 
CCCCAGCTGC ACTGGATCGT GGTGGAAGAT GCTCCTCAGC AAACTCAGCT CGTCTCCGAC 
TTCCTGTCTG CTTCCGGCTT GACCTACACT CATCTAAACA AGCTGACGCC CAAGGAGAGG 
AAGCTGCAGG AGGGAGACCC CAACTGGCTG AAGCCCCGGG GGGCTGAGCA GAGGAACGAG 
GGTCTGCGCT GGCTCAGATG GATGGGTTCT ACAGTTCACG GGAAAGAAGC AGCTGCCCTT 
GAAGAGGCCG TGGTTTACTT TGCTGATGAT GACAACACGT ACAGCCTACA GCTTTTTGAA 
GAGATGCGCT ATACTTACCG TGTGTCAGTG TGGCCTGTGG GTCTGGTGGG TGGGATGAAG 
TTTGAGCGGC CCGTGGTGGA GGATGGGAAG GTTGTGCGTT TCCACACTGG CTGGCGTCCC 
AATCGGCCCT TCCCTATCGA CATGGCTGGA TTTGCAGTGT CCCTGAGGCT GGTACTGACC 
AATAAGGAAG CTTTGTTTGA CGGAGACGCT CAGATGGGCT TCCTGGAAAG CAGTTTCCTT 
CAGCACCTGG TCACTATGGA TGACCTTGAA CCGAAAGCAG ACCTGTGCAC CAAGGTGCTG 
GTTTGGCACA CCCGGACTGA GAAGCCAAAG ATGAAGAGAG AAGAAGCTTT GCTGAAGCAG 
GGGATGGGCT CTGATCCGGA TGTGGAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

On the roles and regulation of chondroitin sulfate and heparan sulfate in zebrafish pharyngeal cartilage morphogenesis.
The Journal of biological chemistry287(40)33905-16(2012 Sep)
Holmborn K,Habicher J,Kasza Z,Eriksson AS,Filipek-Gorniok B,Gopal S,Couchman JR,Ahlberg PE,Wiweger M,Spillmann D,Kreuger J,Ledin J

Growth plate regulation and osteochondroma formation: insights from tracing proteoglycans in zebrafish models and human cartilage.
The Journal of pathology224(2)160-8(2011 Jun)
de Andrea CE,Prins FA,Wiweger MI,Hogendoorn PC

Cartilage ultrastructure in proteoglycan-deficient zebrafish mutants brings to light new candidate genes for human skeletal disorders.
The Journal of pathology223(4)531-42(2011 Mar)
Wiweger MI,Avramut CM,de Andrea CE,Prins FA,Koster AJ,Ravelli RB,Hogendoorn PC