uba7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	uba7
Entrez Gene ID	100001302
Full Name	ubiquitin-like modifier activating enzyme 7
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 7 Names ubiquitin-like modifier-activating enzyme 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155876.3	XP_005155933.1	ubiquitin-like modifier-activating enzyme 1
XM_005155876.4	XP_005155933.1	ubiquitin-like modifier-activating enzyme 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	UBA7	XP_425145.4
Danio rerio (zebrafish)	LOC100001302	XP_001341326.3

Gene Ontology

The following uba7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uba7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12292	XM_005155876.4 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 7 (uba7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa12292	XM_005155876.3	Danio rerio ubiquitin-like modifier activating enzyme 7 (uba7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

uba7 ( XM_005155876.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155933.1 Danio rerio ubiquitin-like modifier activating enzyme 7 (uba7), mRNA.

Price & Availability↑

CloneID	ODa12292
Clone ID Related Accession (Same CDS sequence)	XM_005155876.3 , XM_005155876.4
Accession Version	XM_005155876.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155876.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAG GAGATATTGA TGAGGGGTTA TATTCTCGAC AGCTCTACGT GATTGGCCAT 
GATGCAATGC GTCGTATGGG AAAGGCTGAT GTCCTCATTG CTGGAATGCG AGGTCTTGGC 
GTGGAGATTG CCAAAAACGT GATCCTGGCT GGAGTTAGAA CAGTTACAAT TCAGGATGAG 
GGTGTAGTAG AGTGGAGAGA TTTGTCATCT CAGTTTTATC TGAAGGAAGC TGATCTGGGT 
CAGAACCGGG CTTTGTGCTC TGAGAAACAG TTGTCGAGTC TGAACGCCTA TGTTAAAGTG 
TCCGCCTCGA CTAACAAACT TGATGAGAAC TTCCTTAGCA AGTTCCAGGT TGTGGTGCTC 
ACAAGTTCAC CTTTAGATGA GCAGCTACGT GTGGGAGCGT TTTGTCACTC AAACAATATA 
AAGTTTATTG TGGCTGACAC CAGAGGACTC TGTGGGCAGT TGTTCTGTGA CTTTGGCGAA 
TCGTTTGAAG TCATTGACAC AAATGGAGAT CCTCCAGTTT CAGCCATGAT TTCACATATC 
AGCAAGGAGA ATCCAGGTGT TGTAAATTGC ACCGATGAGG AAAGCCACGA GTTCACTGAT 
GGCATGTTTG TGACTTTTTC TGAGGTTCAA GGCATGACTG AACTCAACAA TTATGGCCCT 
GTTGAGATCA AAGTAAGGGA CAGCTACTCC TTCAGTATTT GTGACACTTC AAACTTTTCT 
GACTATGTGA AGTGTGGAGT GGCAACTGAA GTCAAACAGC CTGAAATCTT GAGCTTTAAA 
CCTCTGAATG TGGCTTTGGA TGAAGCCCTC CGTGATCCCG GTCTTGTAGA GATGACAGAC 
TATGGTAAGA CACAAAGACA CCTGTCTCTT CACCTGGCCT TTCAAGCCCT GCATAAATTC 
ACTCAGAAAT ACAGCCGGAC ACCCCATCCT AGATCTCAGG CGGATGCTGA AGTGCTGCTT 
ACCATCACAA AAGAGCTTTG CACAGAGGCT AAGTTTGATG AGCTTGATGA AGACGCTGTG 
AGGAATTTGT CTCTGGTTGC CAGCGGGGAT CTGGCACCCG TCAATGCCTT CATTGGAGGT 
CTGGCTGCTC AGGAGGTGGT GAAGGCGTGC AGTGGTAAAT TCACTCCTCT CAGACAGTGG 
CTGTACTTCG ATGCGCTGGA ATGTCTTCCT CAAGAGGAGG GTGGAGTTCT TTCTGAAGAT 
GCTTGTGCTC CTAGAGACAG CCGATATGAT GGCCAGATTG CTGTGTTTGG ATCTGATTTT 
CAAAACAAAC TGAAGAAGCA AAAGTATTTT CTGGTTGGAG CTGGAGCAAT TGGTTGTGAG 
CTGCTGAAGA ACTTTGCTCT GATTGGTCTG GGTGCAGGAG AGGGCGGGAG CATTACTGTG 
ACTGACATGG ACTCCATCGA GCGATCAAAT CTGAATCGAC AGTTTCTGTT CCGCTCTCAG 
GACATTGGGA GGCCGAAGTC TGAGGCAGCG GCAGAAGCTG TTAAAGAGAT GAACCCTTTC 
ATGAACATCA TTGCTCAGCA GAACCGTGTG TGTGCAGAGA CGGAGGAGGT TTACACACAT 
TCATTCTACA CTGGTCTGGA TGGTGTGGCT GCAGCGCTGG ATAATGTAGA TGCTCGGGTT 
TATCTTGACC AATGTTGTGT GCGAAACAAA AAGCCCATGC TGGAGGGAGG AACTCTGGGT 
AGCAAAGGCC ACACTATGGT TGTAGTGCCA CGCCTGACCG AATCATACGG ACTCTCCTCA 
TCTGGAGGAC AGAAAGCCAT TCCCATCTGC ACACTCAAGA ACTTCCCTCA CCGTATAGAG 
CACACACTAC AGTGGGCCAG AGACCATTTC GAAGGACTTT TCAAGCAAAC AGCACAGAAC 
GTGAACAACT TTCTCAGTGA TCCAGGGTTT GTGGATCGTA CAGTGGCCCG TGGAGATGTG 
GAGGCTGTGG AGATGCTGGA GGGCGTCTAT AGGAGTCTGA GCGATGACTG GCCTGAGAAC 
TGGAAAGACT GCATCAGCTG GGCCCGCAGA CAATGGGAAA CTCTCTACAA CAATCACATC 
AGACAACTGC TGCACTGCTT TCCTCCTGAC CAGCTAACAA GCTCTGGACT TCCGTTTTGG 
ATGGGTGCAA AGAGATGTCC ACACGCCTTG ACCTTTGACA CTAACAATGC CACCCATATG 
GATTTCATCA TCGCGGCTGC CAATTTATAT GCACAGATTT TTGGGATTAC AGGATCAAGA 
AATCGTGCTG ATATTCAGAC CGTTCTTCAG GGAGTAAAAG TGCCAGAGTT TACACCAAAA 
TCTAGTGTAA AGATAGCTGT GACCGACCAG CAGCTGAATG AGGAAAACGA GGAGAGAAAA 
GAGGAAGATA AAGTCAAGCT GGGGATGTTA AAGGAGCAGC TGTCCAAACT CCAGCTGAGG 
GACCGCAGTT TCCGAATGCA CCCGCAGGAC TTTGAGAAGG ATGATGACAG TAATTTCCAT 
ATGGACTATA TAGTTGCAGC ATCCAACCTG AGGGCAGAAA ACTATGATAT ACCGACAGCC 
GACCGCCACA AAAGCAAGTT GATCGCAGGC CGCATCATTC CAGCAATTGC CACGACAACG 
GCTGCAATCG CAGGACTGAT GTGCTTGGAG CTCTATAAAC TTGTCCAGGG TCACAGCAAA 
ATCACTTCCT ACCGCAACGC TTATATAAAC CTGGCAACCC AGTACTTTGT GTTTTCACAG 
CCTTGTCCAG CCCCCACATT TACCGTCGCG GGTCAGCGTT ACTCTCTCTG GGACGATTTC 
CCGGTCCAGG GCTGTCGTGA GGGTCAGGAG GAAATGACCT TGGAGGAACT GCTGAAACAC 
ATCGAGGAGG AGCATAAGCT GAAGATCAGC GGCTTGTATT ACGGACCTGC TGTGTTGTAC 
AGTGATCTCT CCAATCATAG CGATCGCATG AAGCTGAGCA TTAGTGATTT GGTGCGCCTT 
GCAACCAAGC ATGAAGTTGC AGATCACCAG CAGATGTTGG AGATCATTCC CTCGTTTGAA 
GAGGATGAAG ACTGCCTGAC GGTCCCGCCG ATCAGATACC TGCTCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155933.1
CDS	237..3287
Translation	MAQGDIDEGLYSRQLYVIGHDAMRRMGKADVLIAGMRGLGVEIAKNVILAGVRTVTIQDEGVVEWRDLSSQFYLKEADLGQNRALCSEKQLSSLNAYVKVSASTNKLDENFLSKFQVVVLTSSPLDEQLRVGAFCHSNNIKFIVADTRGLCGQLFCDFGESFEVIDTNGDPPVSAMISHISKENPGVVNCTDEESHEFTDGMFVTFSEVQGMTELNNYGPVEIKVRDSYSFSICDTSNFSDYVKCGVATEVKQPEILSFKPLNVALDEALRDPGLVEMTDYGKTQRHLSLHLAFQALHKFTQKYSRTPHPRSQADAEVLLTITKELCTEAKFDELDEDAVRNLSLVASGDLAPVNAFIGGLAAQEVVKACSGKFTPLRQWLYFDALECLPQEEGGVLSEDACAPRDSRYDGQIAVFGSDFQNKLKKQKYFLVGAGAIGCELLKNFALIGLGAGEGGSITVTDMDSIERSNLNRQFLFRSQDIGRPKSEAAAEAVKEMNPFMNIIAQQNRVCAETEEVYTHSFYTGLDGVAAALDNVDARVYLDQCCVRNKKPMLEGGTLGSKGHTMVVVPRLTESYGLSSSGGQKAIPICTLKNFPHRIEHTLQWARDHFEGLFKQTAQNVNNFLSDPGFVDRTVARGDVEAVEMLEGVYRSLSDDWPENWKDCISWARRQWETLYNNHIRQLLHCFPPDQLTSSGLPFWMGAKRCPHALTFDTNNATHMDFIIAAANLYAQIFGITGSRNRADIQTVLQGVKVPEFTPKSSVKIAVTDQQLNEENEERKEEDKVKLGMLKEQLSKLQLRDRSFRMHPQDFEKDDDSNFHMDYIVAASNLRAENYDIPTADRHKSKLIAGRIIPAIATTTAAIAGLMCLELYKLVQGHSKITSYRNAYINLATQYFVFSQPCPAPTFTVAGQRYSLWDDFPVQGCREGQEEMTLEELLKHIEEEHKLKISGLYYGPAVLYSDLSNHSDRMKLSISDLVRLATKHEVADHQQMLEIIPSFEEDEDCLTVPPIRYLLR

Target ORF information:

RefSeq Version	XM_005155876.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 7 (uba7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155876.3

ORF Insert Sequence:

ATGGCGCAAG GAGATATTGA TGAGGGGTTA TATTCTCGAC AGCTCTACGT GATTGGCCAT 
GATGCAATGC GTCGTATGGG AAAGGCTGAT GTCCTCATTG CTGGAATGCG AGGTCTTGGC 
GTGGAGATTG CCAAAAACGT GATCCTGGCT GGAGTTAGAA CAGTTACAAT TCAGGATGAG 
GGTGTAGTAG AGTGGAGAGA TTTGTCATCT CAGTTTTATC TGAAGGAAGC TGATCTGGGT 
CAGAACCGGG CTTTGTGCTC TGAGAAACAG TTGTCGAGTC TGAACGCCTA TGTTAAAGTG 
TCCGCCTCGA CTAACAAACT TGATGAGAAC TTCCTTAGCA AGTTCCAGGT TGTGGTGCTC 
ACAAGTTCAC CTTTAGATGA GCAGCTACGT GTGGGAGCGT TTTGTCACTC AAACAATATA 
AAGTTTATTG TGGCTGACAC CAGAGGACTC TGTGGGCAGT TGTTCTGTGA CTTTGGCGAA 
TCGTTTGAAG TCATTGACAC AAATGGAGAT CCTCCAGTTT CAGCCATGAT TTCACATATC 
AGCAAGGAGA ATCCAGGTGT TGTAAATTGC ACCGATGAGG AAAGCCACGA GTTCACTGAT 
GGCATGTTTG TGACTTTTTC TGAGGTTCAA GGCATGACTG AACTCAACAA TTATGGCCCT 
GTTGAGATCA AAGTAAGGGA CAGCTACTCC TTCAGTATTT GTGACACTTC AAACTTTTCT 
GACTATGTGA AGTGTGGAGT GGCAACTGAA GTCAAACAGC CTGAAATCTT GAGCTTTAAA 
CCTCTGAATG TGGCTTTGGA TGAAGCCCTC CGTGATCCCG GTCTTGTAGA GATGACAGAC 
TATGGTAAGA CACAAAGACA CCTGTCTCTT CACCTGGCCT TTCAAGCCCT GCATAAATTC 
ACTCAGAAAT ACAGCCGGAC ACCCCATCCT AGATCTCAGG CGGATGCTGA AGTGCTGCTT 
ACCATCACAA AAGAGCTTTG CACAGAGGCT AAGTTTGATG AGCTTGATGA AGACGCTGTG 
AGGAATTTGT CTCTGGTTGC CAGCGGGGAT CTGGCACCCG TCAATGCCTT CATTGGAGGT 
CTGGCTGCTC AGGAGGTGGT GAAGGCGTGC AGTGGTAAAT TCACTCCTCT CAGACAGTGG 
CTGTACTTCG ATGCGCTGGA ATGTCTTCCT CAAGAGGAGG GTGGAGTTCT TTCTGAAGAT 
GCTTGTGCTC CTAGAGACAG CCGATATGAT GGCCAGATTG CTGTGTTTGG ATCTGATTTT 
CAAAACAAAC TGAAGAAGCA AAAGTATTTT CTGGTTGGAG CTGGAGCAAT TGGTTGTGAG 
CTGCTGAAGA ACTTTGCTCT GATTGGTCTG GGTGCAGGAG AGGGCGGGAG CATTACTGTG 
ACTGACATGG ACTCCATCGA GCGATCAAAT CTGAATCGAC AGTTTCTGTT CCGCTCTCAG 
GACATTGGGA GGCCGAAGTC TGAGGCAGCG GCAGAAGCTG TTAAAGAGAT GAACCCTTTC 
ATGAACATCA TTGCTCAGCA GAACCGTGTG TGTGCAGAGA CGGAGGAGGT TTACACACAT 
TCATTCTACA CTGGTCTGGA TGGTGTGGCT GCAGCGCTGG ATAATGTAGA TGCTCGGGTT 
TATCTTGACC AATGTTGTGT GCGAAACAAA AAGCCCATGC TGGAGGGAGG AACTCTGGGT 
AGCAAAGGCC ACACTATGGT TGTAGTGCCA CGCCTGACCG AATCATACGG ACTCTCCTCA 
TCTGGAGGAC AGAAAGCCAT TCCCATCTGC ACACTCAAGA ACTTCCCTCA CCGTATAGAG 
CACACACTAC AGTGGGCCAG AGACCATTTC GAAGGACTTT TCAAGCAAAC AGCACAGAAC 
GTGAACAACT TTCTCAGTGA TCCAGGGTTT GTGGATCGTA CAGTGGCCCG TGGAGATGTG 
GAGGCTGTGG AGATGCTGGA GGGCGTCTAT AGGAGTCTGA GCGATGACTG GCCTGAGAAC 
TGGAAAGACT GCATCAGCTG GGCCCGCAGA CAATGGGAAA CTCTCTACAA CAATCACATC 
AGACAACTGC TGCACTGCTT TCCTCCTGAC CAGCTAACAA GCTCTGGACT TCCGTTTTGG 
ATGGGTGCAA AGAGATGTCC ACACGCCTTG ACCTTTGACA CTAACAATGC CACCCATATG 
GATTTCATCA TCGCGGCTGC CAATTTATAT GCACAGATTT TTGGGATTAC AGGATCAAGA 
AATCGTGCTG ATATTCAGAC CGTTCTTCAG GGAGTAAAAG TGCCAGAGTT TACACCAAAA 
TCTAGTGTAA AGATAGCTGT GACCGACCAG CAGCTGAATG AGGAAAACGA GGAGAGAAAA 
GAGGAAGATA AAGTCAAGCT GGGGATGTTA AAGGAGCAGC TGTCCAAACT CCAGCTGAGG 
GACCGCAGTT TCCGAATGCA CCCGCAGGAC TTTGAGAAGG ATGATGACAG TAATTTCCAT 
ATGGACTATA TAGTTGCAGC ATCCAACCTG AGGGCAGAAA ACTATGATAT ACCGACAGCC 
GACCGCCACA AAAGCAAGTT GATCGCAGGC CGCATCATTC CAGCAATTGC CACGACAACG 
GCTGCAATCG CAGGACTGAT GTGCTTGGAG CTCTATAAAC TTGTCCAGGG TCACAGCAAA 
ATCACTTCCT ACCGCAACGC TTATATAAAC CTGGCAACCC AGTACTTTGT GTTTTCACAG 
CCTTGTCCAG CCCCCACATT TACCGTCGCG GGTCAGCGTT ACTCTCTCTG GGACGATTTC 
CCGGTCCAGG GCTGTCGTGA GGGTCAGGAG GAAATGACCT TGGAGGAACT GCTGAAACAC 
ATCGAGGAGG AGCATAAGCT GAAGATCAGC GGCTTGTATT ACGGACCTGC TGTGTTGTAC 
AGTGATCTCT CCAATCATAG CGATCGCATG AAGCTGAGCA TTAGTGATTT GGTGCGCCTT 
GCAACCAAGC ATGAAGTTGC AGATCACCAG CAGATGTTGG AGATCATTCC CTCGTTTGAA 
GAGGATGAAG ACTGCCTGAC GGTCCCGCCG ATCAGATACC TGCTCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba7 ( XM_005155876.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155933.1 Danio rerio ubiquitin-like modifier activating enzyme 7 (uba7), mRNA.

Price & Availability↑

CloneID	ODa12292
Clone ID Related Accession (Same CDS sequence)	XM_005155876.3 , XM_005155876.4
Accession Version	XM_005155876.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155876.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAG GAGATATTGA TGAGGGGTTA TATTCTCGAC AGCTCTACGT GATTGGCCAT 
GATGCAATGC GTCGTATGGG AAAGGCTGAT GTCCTCATTG CTGGAATGCG AGGTCTTGGC 
GTGGAGATTG CCAAAAACGT GATCCTGGCT GGAGTTAGAA CAGTTACAAT TCAGGATGAG 
GGTGTAGTAG AGTGGAGAGA TTTGTCATCT CAGTTTTATC TGAAGGAAGC TGATCTGGGT 
CAGAACCGGG CTTTGTGCTC TGAGAAACAG TTGTCGAGTC TGAACGCCTA TGTTAAAGTG 
TCCGCCTCGA CTAACAAACT TGATGAGAAC TTCCTTAGCA AGTTCCAGGT TGTGGTGCTC 
ACAAGTTCAC CTTTAGATGA GCAGCTACGT GTGGGAGCGT TTTGTCACTC AAACAATATA 
AAGTTTATTG TGGCTGACAC CAGAGGACTC TGTGGGCAGT TGTTCTGTGA CTTTGGCGAA 
TCGTTTGAAG TCATTGACAC AAATGGAGAT CCTCCAGTTT CAGCCATGAT TTCACATATC 
AGCAAGGAGA ATCCAGGTGT TGTAAATTGC ACCGATGAGG AAAGCCACGA GTTCACTGAT 
GGCATGTTTG TGACTTTTTC TGAGGTTCAA GGCATGACTG AACTCAACAA TTATGGCCCT 
GTTGAGATCA AAGTAAGGGA CAGCTACTCC TTCAGTATTT GTGACACTTC AAACTTTTCT 
GACTATGTGA AGTGTGGAGT GGCAACTGAA GTCAAACAGC CTGAAATCTT GAGCTTTAAA 
CCTCTGAATG TGGCTTTGGA TGAAGCCCTC CGTGATCCCG GTCTTGTAGA GATGACAGAC 
TATGGTAAGA CACAAAGACA CCTGTCTCTT CACCTGGCCT TTCAAGCCCT GCATAAATTC 
ACTCAGAAAT ACAGCCGGAC ACCCCATCCT AGATCTCAGG CGGATGCTGA AGTGCTGCTT 
ACCATCACAA AAGAGCTTTG CACAGAGGCT AAGTTTGATG AGCTTGATGA AGACGCTGTG 
AGGAATTTGT CTCTGGTTGC CAGCGGGGAT CTGGCACCCG TCAATGCCTT CATTGGAGGT 
CTGGCTGCTC AGGAGGTGGT GAAGGCGTGC AGTGGTAAAT TCACTCCTCT CAGACAGTGG 
CTGTACTTCG ATGCGCTGGA ATGTCTTCCT CAAGAGGAGG GTGGAGTTCT TTCTGAAGAT 
GCTTGTGCTC CTAGAGACAG CCGATATGAT GGCCAGATTG CTGTGTTTGG ATCTGATTTT 
CAAAACAAAC TGAAGAAGCA AAAGTATTTT CTGGTTGGAG CTGGAGCAAT TGGTTGTGAG 
CTGCTGAAGA ACTTTGCTCT GATTGGTCTG GGTGCAGGAG AGGGCGGGAG CATTACTGTG 
ACTGACATGG ACTCCATCGA GCGATCAAAT CTGAATCGAC AGTTTCTGTT CCGCTCTCAG 
GACATTGGGA GGCCGAAGTC TGAGGCAGCG GCAGAAGCTG TTAAAGAGAT GAACCCTTTC 
ATGAACATCA TTGCTCAGCA GAACCGTGTG TGTGCAGAGA CGGAGGAGGT TTACACACAT 
TCATTCTACA CTGGTCTGGA TGGTGTGGCT GCAGCGCTGG ATAATGTAGA TGCTCGGGTT 
TATCTTGACC AATGTTGTGT GCGAAACAAA AAGCCCATGC TGGAGGGAGG AACTCTGGGT 
AGCAAAGGCC ACACTATGGT TGTAGTGCCA CGCCTGACCG AATCATACGG ACTCTCCTCA 
TCTGGAGGAC AGAAAGCCAT TCCCATCTGC ACACTCAAGA ACTTCCCTCA CCGTATAGAG 
CACACACTAC AGTGGGCCAG AGACCATTTC GAAGGACTTT TCAAGCAAAC AGCACAGAAC 
GTGAACAACT TTCTCAGTGA TCCAGGGTTT GTGGATCGTA CAGTGGCCCG TGGAGATGTG 
GAGGCTGTGG AGATGCTGGA GGGCGTCTAT AGGAGTCTGA GCGATGACTG GCCTGAGAAC 
TGGAAAGACT GCATCAGCTG GGCCCGCAGA CAATGGGAAA CTCTCTACAA CAATCACATC 
AGACAACTGC TGCACTGCTT TCCTCCTGAC CAGCTAACAA GCTCTGGACT TCCGTTTTGG 
ATGGGTGCAA AGAGATGTCC ACACGCCTTG ACCTTTGACA CTAACAATGC CACCCATATG 
GATTTCATCA TCGCGGCTGC CAATTTATAT GCACAGATTT TTGGGATTAC AGGATCAAGA 
AATCGTGCTG ATATTCAGAC CGTTCTTCAG GGAGTAAAAG TGCCAGAGTT TACACCAAAA 
TCTAGTGTAA AGATAGCTGT GACCGACCAG CAGCTGAATG AGGAAAACGA GGAGAGAAAA 
GAGGAAGATA AAGTCAAGCT GGGGATGTTA AAGGAGCAGC TGTCCAAACT CCAGCTGAGG 
GACCGCAGTT TCCGAATGCA CCCGCAGGAC TTTGAGAAGG ATGATGACAG TAATTTCCAT 
ATGGACTATA TAGTTGCAGC ATCCAACCTG AGGGCAGAAA ACTATGATAT ACCGACAGCC 
GACCGCCACA AAAGCAAGTT GATCGCAGGC CGCATCATTC CAGCAATTGC CACGACAACG 
GCTGCAATCG CAGGACTGAT GTGCTTGGAG CTCTATAAAC TTGTCCAGGG TCACAGCAAA 
ATCACTTCCT ACCGCAACGC TTATATAAAC CTGGCAACCC AGTACTTTGT GTTTTCACAG 
CCTTGTCCAG CCCCCACATT TACCGTCGCG GGTCAGCGTT ACTCTCTCTG GGACGATTTC 
CCGGTCCAGG GCTGTCGTGA GGGTCAGGAG GAAATGACCT TGGAGGAACT GCTGAAACAC 
ATCGAGGAGG AGCATAAGCT GAAGATCAGC GGCTTGTATT ACGGACCTGC TGTGTTGTAC 
AGTGATCTCT CCAATCATAG CGATCGCATG AAGCTGAGCA TTAGTGATTT GGTGCGCCTT 
GCAACCAAGC ATGAAGTTGC AGATCACCAG CAGATGTTGG AGATCATTCC CTCGTTTGAA 
GAGGATGAAG ACTGCCTGAC GGTCCCGCCG ATCAGATACC TGCTCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155933.1
CDS	246..3296
Translation	MAQGDIDEGLYSRQLYVIGHDAMRRMGKADVLIAGMRGLGVEIAKNVILAGVRTVTIQDEGVVEWRDLSSQFYLKEADLGQNRALCSEKQLSSLNAYVKVSASTNKLDENFLSKFQVVVLTSSPLDEQLRVGAFCHSNNIKFIVADTRGLCGQLFCDFGESFEVIDTNGDPPVSAMISHISKENPGVVNCTDEESHEFTDGMFVTFSEVQGMTELNNYGPVEIKVRDSYSFSICDTSNFSDYVKCGVATEVKQPEILSFKPLNVALDEALRDPGLVEMTDYGKTQRHLSLHLAFQALHKFTQKYSRTPHPRSQADAEVLLTITKELCTEAKFDELDEDAVRNLSLVASGDLAPVNAFIGGLAAQEVVKACSGKFTPLRQWLYFDALECLPQEEGGVLSEDACAPRDSRYDGQIAVFGSDFQNKLKKQKYFLVGAGAIGCELLKNFALIGLGAGEGGSITVTDMDSIERSNLNRQFLFRSQDIGRPKSEAAAEAVKEMNPFMNIIAQQNRVCAETEEVYTHSFYTGLDGVAAALDNVDARVYLDQCCVRNKKPMLEGGTLGSKGHTMVVVPRLTESYGLSSSGGQKAIPICTLKNFPHRIEHTLQWARDHFEGLFKQTAQNVNNFLSDPGFVDRTVARGDVEAVEMLEGVYRSLSDDWPENWKDCISWARRQWETLYNNHIRQLLHCFPPDQLTSSGLPFWMGAKRCPHALTFDTNNATHMDFIIAAANLYAQIFGITGSRNRADIQTVLQGVKVPEFTPKSSVKIAVTDQQLNEENEERKEEDKVKLGMLKEQLSKLQLRDRSFRMHPQDFEKDDDSNFHMDYIVAASNLRAENYDIPTADRHKSKLIAGRIIPAIATTTAAIAGLMCLELYKLVQGHSKITSYRNAYINLATQYFVFSQPCPAPTFTVAGQRYSLWDDFPVQGCREGQEEMTLEELLKHIEEEHKLKISGLYYGPAVLYSDLSNHSDRMKLSISDLVRLATKHEVADHQQMLEIIPSFEEDEDCLTVPPIRYLLR

Target ORF information:

RefSeq Version	XM_005155876.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 7 (uba7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155876.4

ORF Insert Sequence:

ATGGCGCAAG GAGATATTGA TGAGGGGTTA TATTCTCGAC AGCTCTACGT GATTGGCCAT 
GATGCAATGC GTCGTATGGG AAAGGCTGAT GTCCTCATTG CTGGAATGCG AGGTCTTGGC 
GTGGAGATTG CCAAAAACGT GATCCTGGCT GGAGTTAGAA CAGTTACAAT TCAGGATGAG 
GGTGTAGTAG AGTGGAGAGA TTTGTCATCT CAGTTTTATC TGAAGGAAGC TGATCTGGGT 
CAGAACCGGG CTTTGTGCTC TGAGAAACAG TTGTCGAGTC TGAACGCCTA TGTTAAAGTG 
TCCGCCTCGA CTAACAAACT TGATGAGAAC TTCCTTAGCA AGTTCCAGGT TGTGGTGCTC 
ACAAGTTCAC CTTTAGATGA GCAGCTACGT GTGGGAGCGT TTTGTCACTC AAACAATATA 
AAGTTTATTG TGGCTGACAC CAGAGGACTC TGTGGGCAGT TGTTCTGTGA CTTTGGCGAA 
TCGTTTGAAG TCATTGACAC AAATGGAGAT CCTCCAGTTT CAGCCATGAT TTCACATATC 
AGCAAGGAGA ATCCAGGTGT TGTAAATTGC ACCGATGAGG AAAGCCACGA GTTCACTGAT 
GGCATGTTTG TGACTTTTTC TGAGGTTCAA GGCATGACTG AACTCAACAA TTATGGCCCT 
GTTGAGATCA AAGTAAGGGA CAGCTACTCC TTCAGTATTT GTGACACTTC AAACTTTTCT 
GACTATGTGA AGTGTGGAGT GGCAACTGAA GTCAAACAGC CTGAAATCTT GAGCTTTAAA 
CCTCTGAATG TGGCTTTGGA TGAAGCCCTC CGTGATCCCG GTCTTGTAGA GATGACAGAC 
TATGGTAAGA CACAAAGACA CCTGTCTCTT CACCTGGCCT TTCAAGCCCT GCATAAATTC 
ACTCAGAAAT ACAGCCGGAC ACCCCATCCT AGATCTCAGG CGGATGCTGA AGTGCTGCTT 
ACCATCACAA AAGAGCTTTG CACAGAGGCT AAGTTTGATG AGCTTGATGA AGACGCTGTG 
AGGAATTTGT CTCTGGTTGC CAGCGGGGAT CTGGCACCCG TCAATGCCTT CATTGGAGGT 
CTGGCTGCTC AGGAGGTGGT GAAGGCGTGC AGTGGTAAAT TCACTCCTCT CAGACAGTGG 
CTGTACTTCG ATGCGCTGGA ATGTCTTCCT CAAGAGGAGG GTGGAGTTCT TTCTGAAGAT 
GCTTGTGCTC CTAGAGACAG CCGATATGAT GGCCAGATTG CTGTGTTTGG ATCTGATTTT 
CAAAACAAAC TGAAGAAGCA AAAGTATTTT CTGGTTGGAG CTGGAGCAAT TGGTTGTGAG 
CTGCTGAAGA ACTTTGCTCT GATTGGTCTG GGTGCAGGAG AGGGCGGGAG CATTACTGTG 
ACTGACATGG ACTCCATCGA GCGATCAAAT CTGAATCGAC AGTTTCTGTT CCGCTCTCAG 
GACATTGGGA GGCCGAAGTC TGAGGCAGCG GCAGAAGCTG TTAAAGAGAT GAACCCTTTC 
ATGAACATCA TTGCTCAGCA GAACCGTGTG TGTGCAGAGA CGGAGGAGGT TTACACACAT 
TCATTCTACA CTGGTCTGGA TGGTGTGGCT GCAGCGCTGG ATAATGTAGA TGCTCGGGTT 
TATCTTGACC AATGTTGTGT GCGAAACAAA AAGCCCATGC TGGAGGGAGG AACTCTGGGT 
AGCAAAGGCC ACACTATGGT TGTAGTGCCA CGCCTGACCG AATCATACGG ACTCTCCTCA 
TCTGGAGGAC AGAAAGCCAT TCCCATCTGC ACACTCAAGA ACTTCCCTCA CCGTATAGAG 
CACACACTAC AGTGGGCCAG AGACCATTTC GAAGGACTTT TCAAGCAAAC AGCACAGAAC 
GTGAACAACT TTCTCAGTGA TCCAGGGTTT GTGGATCGTA CAGTGGCCCG TGGAGATGTG 
GAGGCTGTGG AGATGCTGGA GGGCGTCTAT AGGAGTCTGA GCGATGACTG GCCTGAGAAC 
TGGAAAGACT GCATCAGCTG GGCCCGCAGA CAATGGGAAA CTCTCTACAA CAATCACATC 
AGACAACTGC TGCACTGCTT TCCTCCTGAC CAGCTAACAA GCTCTGGACT TCCGTTTTGG 
ATGGGTGCAA AGAGATGTCC ACACGCCTTG ACCTTTGACA CTAACAATGC CACCCATATG 
GATTTCATCA TCGCGGCTGC CAATTTATAT GCACAGATTT TTGGGATTAC AGGATCAAGA 
AATCGTGCTG ATATTCAGAC CGTTCTTCAG GGAGTAAAAG TGCCAGAGTT TACACCAAAA 
TCTAGTGTAA AGATAGCTGT GACCGACCAG CAGCTGAATG AGGAAAACGA GGAGAGAAAA 
GAGGAAGATA AAGTCAAGCT GGGGATGTTA AAGGAGCAGC TGTCCAAACT CCAGCTGAGG 
GACCGCAGTT TCCGAATGCA CCCGCAGGAC TTTGAGAAGG ATGATGACAG TAATTTCCAT 
ATGGACTATA TAGTTGCAGC ATCCAACCTG AGGGCAGAAA ACTATGATAT ACCGACAGCC 
GACCGCCACA AAAGCAAGTT GATCGCAGGC CGCATCATTC CAGCAATTGC CACGACAACG 
GCTGCAATCG CAGGACTGAT GTGCTTGGAG CTCTATAAAC TTGTCCAGGG TCACAGCAAA 
ATCACTTCCT ACCGCAACGC TTATATAAAC CTGGCAACCC AGTACTTTGT GTTTTCACAG 
CCTTGTCCAG CCCCCACATT TACCGTCGCG GGTCAGCGTT ACTCTCTCTG GGACGATTTC 
CCGGTCCAGG GCTGTCGTGA GGGTCAGGAG GAAATGACCT TGGAGGAACT GCTGAAACAC 
ATCGAGGAGG AGCATAAGCT GAAGATCAGC GGCTTGTATT ACGGACCTGC TGTGTTGTAC 
AGTGATCTCT CCAATCATAG CGATCGCATG AAGCTGAGCA TTAGTGATTT GGTGCGCCTT 
GCAACCAAGC ATGAAGTTGC AGATCACCAG CAGATGTTGG AGATCATTCC CTCGTTTGAA 
GAGGATGAAG ACTGCCTGAC GGTCCCGCCG ATCAGATACC TGCTCAGGTG A