VCL cDNA ORF clone, Crocodylus porosus(Australian saltwater crocodile)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	109323907
Full Name	vinculin
Gene Type	protein-coding
Organism	Crocodylus porosus(Australian saltwater crocodile)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019555556.1	XP_019411101.1	vinculin isoform X1
XM_019555557.1	XP_019411102.1	vinculin isoform X2

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr85466	XM_019555556.1 Latest version!	Crocodylus porosus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCr85467	XM_019555557.1 Latest version!	Crocodylus porosus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_019555556.1 ) cDNA ORF clone, Crocodylus porosus(Australian saltwater crocodile) -> XP_019411101.1 Crocodylus porosus vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr85466
Clone ID Related Accession (Same CDS sequence)	XM_019555556.1
Accession Version	XM_019555556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-19
Organism	Crocodylus porosus(Australian saltwater crocodile)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017728951.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crocodylus porosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTCT TCCATACCCG CACCATTGAG AGCATCCTGG AGCCCGTGGC GCAGCAGATC 
TCGCACCTCG TCATCATGCA CGAGGAGGGC GAGGTGGACG GCAAGGCCAT CCCAGACCTC 
ACTGCCCCAG TGGCCGCTGT GCAGGCCGCC GTCAGCAACC TGGTCCGGGT TGGAAAAGAG 
ACTGTGCAGA CAACTGAAGA CCAGATCTTG AAAAGGGACA TGCCACCAGC ATTTATCAAG 
GTGGAGAATG CCTGCACTAA ACTTGTCCAA GCGGCCCAGA TGCTACAAGC AGATCCTTAT 
TCAGTGCCAG CTCGTGATTA TCTCATTGAT GGATCAAGAG GCATACTTTC TGGAACATCA 
GACTTGCTCC TGACTTTTGA TGAGGCTGAG GTCCGTAAAA TCATCCGTGT CTGCAAGGGA 
ATTTTGGAAT ATCTAACTGT GGCAGAGGTG GTGGAGACTA TGGAGGATTT AGTGACATAC 
ACAAAGAATT TGGGGCCAGG AATGACAAAA ATGGCAAAAA TGATTGATGA AAGACAGCAG 
GAATTGACTC ATCAGGAGCA CAGAGTCATG TTGGTGAATT CCATGAACAC TGTGAAGGAG 
CTATTGCCAG TCCTTATTTC AGCTATGAAG ATTTTTGTAA CTACTAAAAA TTCTAAAAGC 
CAAGGGATAG AAGAGGCTTT GAAAAACCGT AACTTCACAG TAGAGAAGAT GAGTGCCGAG 
ATTAATGAAA TCATCCGTGT GTTGCAACTC ACTTCCTGGG ATGAGGATGC CTGGGCTAGT 
AAGGACACTG AAGCCATGAA GAGAGCTTTG GCCTTAATCG ATTCCAAAAT GAACCAGGCA 
AAAGGATGGC TAAGGGATCC TAATGCACCA GCAGGTGATG CTGGTGAGCA AGCAATAAGG 
CAAATCCTTG ATGAAGCTGG AAAAGTGGGA GAACTGTGTG CAGGCAAAGA GCGCAGAGAG 
ATTCTGGGAA CCTGTAAAAT GCTGGGCCAG ATGACTGATC AAGTAGCTGA TCTTCGAGCC 
AGAGGACAAG GTGCTACACC AATGGCCATG CAGAAAGCAC AGCAAGTGTC TCAAGGTCTG 
GATTTGTTGA CAGCAAAAGT GGAAAATGCA GCCCGTAAAC TGGAGGCCAT GACTAACTCT 
AAGCAGGCAA TTGCTAAAAA AATAGATGCT GCTCAGAACT GGCTTGCAGA TCCTAATGGG 
GGCAGTGAAG GAGAAGAACA TATTCGCGGA ATTATGGCTG AAGCCAGGAA AATTGCAGAA 
CTTTGTGAGG AACCCAAGGA AAGAGATGAC ATTCTGCGTT CCTTGGGGGA AATCTCTGCT 
CTTACTGCCA AGCTGTCAGA TCTACGAAGA CATGGGAAAG GAGACTCTCC AGAGGCCCGT 
GCATTGGCTA AGCAGATAGC CACATCACTG CAGAACTTAC AGACTAAAAC TAACAAAGTT 
GTAGCAAACA CTAGGCCAGT TAAGGCTGCA GTCCATTTGG AGGGCAAGAT TGAACAAGCT 
CAAAGGTGGA TTGACAATCC GACAGTTGAT GATCGTGGAG TAGGCCAGGC TGCAATCCGG 
GGACTGGTTG CTGAAGGTCG TCGTTTAGCC AATGTTATGA TGGGACCCTA TCGCCAAGAT 
CTGCTTGCCA AATGTGACCG TGTAGAACAG CTGGCTGCCC AGCTTGCTGA TCTGGCAGCT 
AGAGGAGAAG GAGAGTCTCC TCAGGCAAGG GCTATTGCTG CACAACTCCA GGACTCCTTG 
AAGGATCTGA AAACAAGGAT GCAGGAAGCA ATGACTCAGG AAGTTTCTGA TGTCTTCAGT 
GACACTACAA CTCCTATTAA ACTATTGGCA GTGGCAGCCA CAGCACCTCC TGATGCGCCC 
AATAGAGATG AGGTATTTGG TGAAAGAGCA GCGAACTTTG AAAACCATGC TGCTAGACTT 
GGAGCTACAG CAGAAAAAGC AGCTGCTGTG GGAACCGCTA ATAAAACTAC TGTGGAAGGC 
ATTCAAGCAA CTGTGAAATC AGCGAGAGAA CTTACACCTC AGGTAGTATC AGCTGCTCGT 
ATTCTGCTAA GAAATCCTGG CAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAAAAT 
CAATGGATTG ATAATGTGGA AAAAATGACA GGGTTGGTGG ATGAAGCAAT TGATACCAAG 
TCTCTGTTGG ATGCATCAGA AGAAGCCATC AAGAAAGACC TTGATAAATG TAAAGTTGCA 
ATGGCTAATA TCCAACCTCA GATGCTCGTA GCCGGTGCCA CCAGCATTGC TCGGAGGGCC 
AATCGGATCT TACTGGTAGC AAAACGGGAG GTTGAAAATT CGGAGGATCC CAAATTCCGG 
GAAGCTGTTA AGGCAGCCTC TGATGACCTC AGCAAAACTA TATCGCCAAT GGTAATGGAT 
GCTAAAGCTG TAGCGGGGAA CATTTCTGAT CCAGGTTTGC AGAAGAGCTT TCTGGATTCT 
GGATATAGGA TTCTGGGAGC TGTAGCAAAA GTCAGGGAAG CCTTCCAGCC TCAAGAACCT 
GATTTCCCCC CTCCTCCACC AGACCTTGAG CAGCTTCATC TAACTGATGA ACTTGCTCCT 
CCGAAACCAC CCCTTCCTGA GGGTGAAGTT CCCCCACCCA GACCACCTCC TCCAGAAGAG 
AAGGATGAAG AGTTCCCAGA GCAGAAGGCT GGAGAAGTGA TTAATCAACC CATGATGATG 
GCTGCTAGGC AGCTACATGA TGAAGCCCGA AAATGGTCCA GCAAGGGTAA TGACATCATT 
GCTGCTGCCA AAAGAATGGC TCTGCTAATG GCAGAGATGT CCCGCCTTGT GAGAGGTGGC 
AGTGGGACCA AGCGTGCCCT TATTCAGTGT GCGAAGGATA TTGCAAAAGC ATCAGATGAA 
GTAACTCGAC TGGCCAAGGA GGTGGCAAAA CAGTGCACTG ACAAACGTAT TCGAACCAAC 
CTCTTGCAGG TCTGTGAGCG AATCCCAACC ATCAGTACAC AACTCAAAAT CCTCTCTACT 
GTCAAAGCTA CCATGCTGGG CAGAACTAAC ATCAGTGATG AAGAGTCAGA ACAGGCTACT 
GAAATGTTGG TTCATAATGC CCAGAACCTT ATGCAATCTG TAAAGGAGAC CGTGAGAGAA 
GCAGAAGCAG CATCTATTAA GATTAGAACA GATGCTGGAT TCACCCTTCG CTGGGTTAGA 
AAGACTCCCT GGTATCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019411101.1
CDS	165..3365
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPAGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMAEARKIAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQTKTNKVVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVEQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKTRMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNRDEVFGERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDDLSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLHLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_019555556.1
Organism	Crocodylus porosus(Australian saltwater crocodile)
Definition	Crocodylus porosus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019555556.1

ORF Insert Sequence:

ATGCCCGTCT TCCATACCCG CACCATTGAG AGCATCCTGG AGCCCGTGGC GCAGCAGATC 
TCGCACCTCG TCATCATGCA CGAGGAGGGC GAGGTGGACG GCAAGGCCAT CCCAGACCTC 
ACTGCCCCAG TGGCCGCTGT GCAGGCCGCC GTCAGCAACC TGGTCCGGGT TGGAAAAGAG 
ACTGTGCAGA CAACTGAAGA CCAGATCTTG AAAAGGGACA TGCCACCAGC ATTTATCAAG 
GTGGAGAATG CCTGCACTAA ACTTGTCCAA GCGGCCCAGA TGCTACAAGC AGATCCTTAT 
TCAGTGCCAG CTCGTGATTA TCTCATTGAT GGATCAAGAG GCATACTTTC TGGAACATCA 
GACTTGCTCC TGACTTTTGA TGAGGCTGAG GTCCGTAAAA TCATCCGTGT CTGCAAGGGA 
ATTTTGGAAT ATCTAACTGT GGCAGAGGTG GTGGAGACTA TGGAGGATTT AGTGACATAC 
ACAAAGAATT TGGGGCCAGG AATGACAAAA ATGGCAAAAA TGATTGATGA AAGACAGCAG 
GAATTGACTC ATCAGGAGCA CAGAGTCATG TTGGTGAATT CCATGAACAC TGTGAAGGAG 
CTATTGCCAG TCCTTATTTC AGCTATGAAG ATTTTTGTAA CTACTAAAAA TTCTAAAAGC 
CAAGGGATAG AAGAGGCTTT GAAAAACCGT AACTTCACAG TAGAGAAGAT GAGTGCCGAG 
ATTAATGAAA TCATCCGTGT GTTGCAACTC ACTTCCTGGG ATGAGGATGC CTGGGCTAGT 
AAGGACACTG AAGCCATGAA GAGAGCTTTG GCCTTAATCG ATTCCAAAAT GAACCAGGCA 
AAAGGATGGC TAAGGGATCC TAATGCACCA GCAGGTGATG CTGGTGAGCA AGCAATAAGG 
CAAATCCTTG ATGAAGCTGG AAAAGTGGGA GAACTGTGTG CAGGCAAAGA GCGCAGAGAG 
ATTCTGGGAA CCTGTAAAAT GCTGGGCCAG ATGACTGATC AAGTAGCTGA TCTTCGAGCC 
AGAGGACAAG GTGCTACACC AATGGCCATG CAGAAAGCAC AGCAAGTGTC TCAAGGTCTG 
GATTTGTTGA CAGCAAAAGT GGAAAATGCA GCCCGTAAAC TGGAGGCCAT GACTAACTCT 
AAGCAGGCAA TTGCTAAAAA AATAGATGCT GCTCAGAACT GGCTTGCAGA TCCTAATGGG 
GGCAGTGAAG GAGAAGAACA TATTCGCGGA ATTATGGCTG AAGCCAGGAA AATTGCAGAA 
CTTTGTGAGG AACCCAAGGA AAGAGATGAC ATTCTGCGTT CCTTGGGGGA AATCTCTGCT 
CTTACTGCCA AGCTGTCAGA TCTACGAAGA CATGGGAAAG GAGACTCTCC AGAGGCCCGT 
GCATTGGCTA AGCAGATAGC CACATCACTG CAGAACTTAC AGACTAAAAC TAACAAAGTT 
GTAGCAAACA CTAGGCCAGT TAAGGCTGCA GTCCATTTGG AGGGCAAGAT TGAACAAGCT 
CAAAGGTGGA TTGACAATCC GACAGTTGAT GATCGTGGAG TAGGCCAGGC TGCAATCCGG 
GGACTGGTTG CTGAAGGTCG TCGTTTAGCC AATGTTATGA TGGGACCCTA TCGCCAAGAT 
CTGCTTGCCA AATGTGACCG TGTAGAACAG CTGGCTGCCC AGCTTGCTGA TCTGGCAGCT 
AGAGGAGAAG GAGAGTCTCC TCAGGCAAGG GCTATTGCTG CACAACTCCA GGACTCCTTG 
AAGGATCTGA AAACAAGGAT GCAGGAAGCA ATGACTCAGG AAGTTTCTGA TGTCTTCAGT 
GACACTACAA CTCCTATTAA ACTATTGGCA GTGGCAGCCA CAGCACCTCC TGATGCGCCC 
AATAGAGATG AGGTATTTGG TGAAAGAGCA GCGAACTTTG AAAACCATGC TGCTAGACTT 
GGAGCTACAG CAGAAAAAGC AGCTGCTGTG GGAACCGCTA ATAAAACTAC TGTGGAAGGC 
ATTCAAGCAA CTGTGAAATC AGCGAGAGAA CTTACACCTC AGGTAGTATC AGCTGCTCGT 
ATTCTGCTAA GAAATCCTGG CAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAAAAT 
CAATGGATTG ATAATGTGGA AAAAATGACA GGGTTGGTGG ATGAAGCAAT TGATACCAAG 
TCTCTGTTGG ATGCATCAGA AGAAGCCATC AAGAAAGACC TTGATAAATG TAAAGTTGCA 
ATGGCTAATA TCCAACCTCA GATGCTCGTA GCCGGTGCCA CCAGCATTGC TCGGAGGGCC 
AATCGGATCT TACTGGTAGC AAAACGGGAG GTTGAAAATT CGGAGGATCC CAAATTCCGG 
GAAGCTGTTA AGGCAGCCTC TGATGACCTC AGCAAAACTA TATCGCCAAT GGTAATGGAT 
GCTAAAGCTG TAGCGGGGAA CATTTCTGAT CCAGGTTTGC AGAAGAGCTT TCTGGATTCT 
GGATATAGGA TTCTGGGAGC TGTAGCAAAA GTCAGGGAAG CCTTCCAGCC TCAAGAACCT 
GATTTCCCCC CTCCTCCACC AGACCTTGAG CAGCTTCATC TAACTGATGA ACTTGCTCCT 
CCGAAACCAC CCCTTCCTGA GGGTGAAGTT CCCCCACCCA GACCACCTCC TCCAGAAGAG 
AAGGATGAAG AGTTCCCAGA GCAGAAGGCT GGAGAAGTGA TTAATCAACC CATGATGATG 
GCTGCTAGGC AGCTACATGA TGAAGCCCGA AAATGGTCCA GCAAGGGTAA TGACATCATT 
GCTGCTGCCA AAAGAATGGC TCTGCTAATG GCAGAGATGT CCCGCCTTGT GAGAGGTGGC 
AGTGGGACCA AGCGTGCCCT TATTCAGTGT GCGAAGGATA TTGCAAAAGC ATCAGATGAA 
GTAACTCGAC TGGCCAAGGA GGTGGCAAAA CAGTGCACTG ACAAACGTAT TCGAACCAAC 
CTCTTGCAGG TCTGTGAGCG AATCCCAACC ATCAGTACAC AACTCAAAAT CCTCTCTACT 
GTCAAAGCTA CCATGCTGGG CAGAACTAAC ATCAGTGATG AAGAGTCAGA ACAGGCTACT 
GAAATGTTGG TTCATAATGC CCAGAACCTT ATGCAATCTG TAAAGGAGAC CGTGAGAGAA 
GCAGAAGCAG CATCTATTAA GATTAGAACA GATGCTGGAT TCACCCTTCG CTGGGTTAGA 
AAGACTCCCT GGTATCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_019555557.1 ) cDNA ORF clone, Crocodylus porosus(Australian saltwater crocodile) -> XP_019411102.1 Crocodylus porosus vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr85467
Clone ID Related Accession (Same CDS sequence)	XM_019555557.1
Accession Version	XM_019555557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-19
Organism	Crocodylus porosus(Australian saltwater crocodile)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017728951.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crocodylus porosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTCT TCCATACCCG CACCATTGAG AGCATCCTGG AGCCCGTGGC GCAGCAGATC 
TCGCACCTCG TCATCATGCA CGAGGAGGGC GAGGTGGACG GCAAGGCCAT CCCAGACCTC 
ACTGCCCCAG TGGCCGCTGT GCAGGCCGCC GTCAGCAACC TGGTCCGGGT TGGAAAAGAG 
ACTGTGCAGA CAACTGAAGA CCAGATCTTG AAAAGGGACA TGCCACCAGC ATTTATCAAG 
GTGGAGAATG CCTGCACTAA ACTTGTCCAA GCGGCCCAGA TGCTACAAGC AGATCCTTAT 
TCAGTGCCAG CTCGTGATTA TCTCATTGAT GGATCAAGAG GCATACTTTC TGGAACATCA 
GACTTGCTCC TGACTTTTGA TGAGGCTGAG GTCCGTAAAA TCATCCGTGT CTGCAAGGGA 
ATTTTGGAAT ATCTAACTGT GGCAGAGGTG GTGGAGACTA TGGAGGATTT AGTGACATAC 
ACAAAGAATT TGGGGCCAGG AATGACAAAA ATGGCAAAAA TGATTGATGA AAGACAGCAG 
GAATTGACTC ATCAGGAGCA CAGAGTCATG TTGGTGAATT CCATGAACAC TGTGAAGGAG 
CTATTGCCAG TCCTTATTTC AGCTATGAAG ATTTTTGTAA CTACTAAAAA TTCTAAAAGC 
CAAGGGATAG AAGAGGCTTT GAAAAACCGT AACTTCACAG TAGAGAAGAT GAGTGCCGAG 
ATTAATGAAA TCATCCGTGT GTTGCAACTC ACTTCCTGGG ATGAGGATGC CTGGGCTAGT 
AAGGACACTG AAGCCATGAA GAGAGCTTTG GCCTTAATCG ATTCCAAAAT GAACCAGGCA 
AAAGGATGGC TAAGGGATCC TAATGCACCA GCAGGTGATG CTGGTGAGCA AGCAATAAGG 
CAAATCCTTG ATGAAGCTGG AAAAGTGGGA GAACTGTGTG CAGGCAAAGA GCGCAGAGAG 
ATTCTGGGAA CCTGTAAAAT GCTGGGCCAG ATGACTGATC AAGTAGCTGA TCTTCGAGCC 
AGAGGACAAG GTGCTACACC AATGGCCATG CAGAAAGCAC AGCAAGTGTC TCAAGGTCTG 
GATTTGTTGA CAGCAAAAGT GGAAAATGCA GCCCGTAAAC TGGAGGCCAT GACTAACTCT 
AAGCAGGCAA TTGCTAAAAA AATAGATGCT GCTCAGAACT GGCTTGCAGA TCCTAATGGG 
GGCAGTGAAG GAGAAGAACA TATTCGCGGA ATTATGGCTG AAGCCAGGAA AATTGCAGAA 
CTTTGTGAGG AACCCAAGGA AAGAGATGAC ATTCTGCGTT CCTTGGGGGA AATCTCTGCT 
CTTACTGCCA AGCTGTCAGA TCTACGAAGA CATGGGAAAG GAGACTCTCC AGAGGCCCGT 
GCATTGGCTA AGCAGATAGC CACATCACTG CAGAACTTAC AGACTAAAAC TAACAAAGTT 
GTAGCAAACA CTAGGCCAGT TAAGGCTGCA GTCCATTTGG AGGGCAAGAT TGAACAAGCT 
CAAAGGTGGA TTGACAATCC GACAGTTGAT GATCGTGGAG TAGGCCAGGC TGCAATCCGG 
GGACTGGTTG CTGAAGGTCG TCGTTTAGCC AATGTTATGA TGGGACCCTA TCGCCAAGAT 
CTGCTTGCCA AATGTGACCG TGTAGAACAG CTGGCTGCCC AGCTTGCTGA TCTGGCAGCT 
AGAGGAGAAG GAGAGTCTCC TCAGGCAAGG GCTATTGCTG CACAACTCCA GGACTCCTTG 
AAGGATCTGA AAACAAGGAT GCAGGAAGCA ATGACTCAGG AAGTTTCTGA TGTCTTCAGT 
GACACTACAA CTCCTATTAA ACTATTGGCA GTGGCAGCCA CAGCACCTCC TGATGCGCCC 
AATAGAGATG AGGTATTTGG TGAAAGAGCA GCGAACTTTG AAAACCATGC TGCTAGACTT 
GGAGCTACAG CAGAAAAAGC AGCTGCTGTG GGAACCGCTA ATAAAACTAC TGTGGAAGGC 
ATTCAAGCAA CTGTGAAATC AGCGAGAGAA CTTACACCTC AGGTAGTATC AGCTGCTCGT 
ATTCTGCTAA GAAATCCTGG CAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAAAAT 
CAATGGATTG ATAATGTGGA AAAAATGACA GGGTTGGTGG ATGAAGCAAT TGATACCAAG 
TCTCTGTTGG ATGCATCAGA AGAAGCCATC AAGAAAGACC TTGATAAATG TAAAGTTGCA 
ATGGCTAATA TCCAACCTCA GATGCTCGTA GCCGGTGCCA CCAGCATTGC TCGGAGGGCC 
AATCGGATCT TACTGGTAGC AAAACGGGAG GTTGAAAATT CGGAGGATCC CAAATTCCGG 
GAAGCTGTTA AGGCAGCCTC TGATGACCTC AGCAAAACTA TATCGCCAAT GGTAATGGAT 
GCTAAAGCTG TAGCGGGGAA CATTTCTGAT CCAGGTTTGC AGAAGAGCTT TCTGGATTCT 
GGATATAGGA TTCTGGGAGC TGTAGCAAAA GTCAGGGAAG CCTTCCAGCC TCAAGAACCT 
GATTTCCCCC CTCCTCCACC AGACCTTGAG CAGCTTCATC TAACTGATGA ACTTGCTCCT 
CCGAAACCAC CCCTTCCTGA GGGTGAAGTT CCCCCACCCA GACCACCTCC TCCAGAAGAG 
AAGGATGAAG AGTTCCCAGA GCAGAAGGCT GGAGAAGTGA TTAATCAACC CATGATGATG 
GCTGCTAGGC AGCTACATGA TGAAGCCCGA AAATGGTCCA GCAAGCCTGA TGTGACTGGG 
GTTAATGAAG CCACTGAGGC TGGTGTAGAT ATAGATGAGG AGGATGATGC AGATGATGCT 
GAATTCACCC TCCCTTCTGA CATTGAAGAT GATTACGAGC CTGAGCTGCT GTTAATACCA 
AGCAATCAGC CCGTTAACCA GCCCATTCTG GCCGCTGCTC AGTCTCTACA TCGGGAAGCA 
ACCAAGTGGT CTAGTAAGGG TAATGACATC ATTGCTGCTG CCAAAAGAAT GGCTCTGCTA 
ATGGCAGAGA TGTCCCGCCT TGTGAGAGGT GGCAGTGGGA CCAAGCGTGC CCTTATTCAG 
TGTGCGAAGG ATATTGCAAA AGCATCAGAT GAAGTAACTC GACTGGCCAA GGAGGTGGCA 
AAACAGTGCA CTGACAAACG TATTCGAACC AACCTCTTGC AGGTCTGTGA GCGAATCCCA 
ACCATCAGTA CACAACTCAA AATCCTCTCT ACTGTCAAAG CTACCATGCT GGGCAGAACT 
AACATCAGTG ATGAAGAGTC AGAACAGGCT ACTGAAATGT TGGTTCATAA TGCCCAGAAC 
CTTATGCAAT CTGTAAAGGA GACCGTGAGA GAAGCAGAAG CAGCATCTAT TAAGATTAGA 
ACAGATGCTG GATTCACCCT TCGCTGGGTT AGAAAGACTC CCTGGTATCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019411102.1
CDS	167..3580
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPAGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMAEARKIAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQTKTNKVVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVEQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKTRMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNRDEVFGERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDDLSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLHLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPDVTGVNEATEAGVDIDEEDDADDAEFTLPSDIEDDYEPELLLIPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_019555557.1
Organism	Crocodylus porosus(Australian saltwater crocodile)
Definition	Crocodylus porosus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019555557.1

ORF Insert Sequence:

ATGCCCGTCT TCCATACCCG CACCATTGAG AGCATCCTGG AGCCCGTGGC GCAGCAGATC 
TCGCACCTCG TCATCATGCA CGAGGAGGGC GAGGTGGACG GCAAGGCCAT CCCAGACCTC 
ACTGCCCCAG TGGCCGCTGT GCAGGCCGCC GTCAGCAACC TGGTCCGGGT TGGAAAAGAG 
ACTGTGCAGA CAACTGAAGA CCAGATCTTG AAAAGGGACA TGCCACCAGC ATTTATCAAG 
GTGGAGAATG CCTGCACTAA ACTTGTCCAA GCGGCCCAGA TGCTACAAGC AGATCCTTAT 
TCAGTGCCAG CTCGTGATTA TCTCATTGAT GGATCAAGAG GCATACTTTC TGGAACATCA 
GACTTGCTCC TGACTTTTGA TGAGGCTGAG GTCCGTAAAA TCATCCGTGT CTGCAAGGGA 
ATTTTGGAAT ATCTAACTGT GGCAGAGGTG GTGGAGACTA TGGAGGATTT AGTGACATAC 
ACAAAGAATT TGGGGCCAGG AATGACAAAA ATGGCAAAAA TGATTGATGA AAGACAGCAG 
GAATTGACTC ATCAGGAGCA CAGAGTCATG TTGGTGAATT CCATGAACAC TGTGAAGGAG 
CTATTGCCAG TCCTTATTTC AGCTATGAAG ATTTTTGTAA CTACTAAAAA TTCTAAAAGC 
CAAGGGATAG AAGAGGCTTT GAAAAACCGT AACTTCACAG TAGAGAAGAT GAGTGCCGAG 
ATTAATGAAA TCATCCGTGT GTTGCAACTC ACTTCCTGGG ATGAGGATGC CTGGGCTAGT 
AAGGACACTG AAGCCATGAA GAGAGCTTTG GCCTTAATCG ATTCCAAAAT GAACCAGGCA 
AAAGGATGGC TAAGGGATCC TAATGCACCA GCAGGTGATG CTGGTGAGCA AGCAATAAGG 
CAAATCCTTG ATGAAGCTGG AAAAGTGGGA GAACTGTGTG CAGGCAAAGA GCGCAGAGAG 
ATTCTGGGAA CCTGTAAAAT GCTGGGCCAG ATGACTGATC AAGTAGCTGA TCTTCGAGCC 
AGAGGACAAG GTGCTACACC AATGGCCATG CAGAAAGCAC AGCAAGTGTC TCAAGGTCTG 
GATTTGTTGA CAGCAAAAGT GGAAAATGCA GCCCGTAAAC TGGAGGCCAT GACTAACTCT 
AAGCAGGCAA TTGCTAAAAA AATAGATGCT GCTCAGAACT GGCTTGCAGA TCCTAATGGG 
GGCAGTGAAG GAGAAGAACA TATTCGCGGA ATTATGGCTG AAGCCAGGAA AATTGCAGAA 
CTTTGTGAGG AACCCAAGGA AAGAGATGAC ATTCTGCGTT CCTTGGGGGA AATCTCTGCT 
CTTACTGCCA AGCTGTCAGA TCTACGAAGA CATGGGAAAG GAGACTCTCC AGAGGCCCGT 
GCATTGGCTA AGCAGATAGC CACATCACTG CAGAACTTAC AGACTAAAAC TAACAAAGTT 
GTAGCAAACA CTAGGCCAGT TAAGGCTGCA GTCCATTTGG AGGGCAAGAT TGAACAAGCT 
CAAAGGTGGA TTGACAATCC GACAGTTGAT GATCGTGGAG TAGGCCAGGC TGCAATCCGG 
GGACTGGTTG CTGAAGGTCG TCGTTTAGCC AATGTTATGA TGGGACCCTA TCGCCAAGAT 
CTGCTTGCCA AATGTGACCG TGTAGAACAG CTGGCTGCCC AGCTTGCTGA TCTGGCAGCT 
AGAGGAGAAG GAGAGTCTCC TCAGGCAAGG GCTATTGCTG CACAACTCCA GGACTCCTTG 
AAGGATCTGA AAACAAGGAT GCAGGAAGCA ATGACTCAGG AAGTTTCTGA TGTCTTCAGT 
GACACTACAA CTCCTATTAA ACTATTGGCA GTGGCAGCCA CAGCACCTCC TGATGCGCCC 
AATAGAGATG AGGTATTTGG TGAAAGAGCA GCGAACTTTG AAAACCATGC TGCTAGACTT 
GGAGCTACAG CAGAAAAAGC AGCTGCTGTG GGAACCGCTA ATAAAACTAC TGTGGAAGGC 
ATTCAAGCAA CTGTGAAATC AGCGAGAGAA CTTACACCTC AGGTAGTATC AGCTGCTCGT 
ATTCTGCTAA GAAATCCTGG CAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAAAAT 
CAATGGATTG ATAATGTGGA AAAAATGACA GGGTTGGTGG ATGAAGCAAT TGATACCAAG 
TCTCTGTTGG ATGCATCAGA AGAAGCCATC AAGAAAGACC TTGATAAATG TAAAGTTGCA 
ATGGCTAATA TCCAACCTCA GATGCTCGTA GCCGGTGCCA CCAGCATTGC TCGGAGGGCC 
AATCGGATCT TACTGGTAGC AAAACGGGAG GTTGAAAATT CGGAGGATCC CAAATTCCGG 
GAAGCTGTTA AGGCAGCCTC TGATGACCTC AGCAAAACTA TATCGCCAAT GGTAATGGAT 
GCTAAAGCTG TAGCGGGGAA CATTTCTGAT CCAGGTTTGC AGAAGAGCTT TCTGGATTCT 
GGATATAGGA TTCTGGGAGC TGTAGCAAAA GTCAGGGAAG CCTTCCAGCC TCAAGAACCT 
GATTTCCCCC CTCCTCCACC AGACCTTGAG CAGCTTCATC TAACTGATGA ACTTGCTCCT 
CCGAAACCAC CCCTTCCTGA GGGTGAAGTT CCCCCACCCA GACCACCTCC TCCAGAAGAG 
AAGGATGAAG AGTTCCCAGA GCAGAAGGCT GGAGAAGTGA TTAATCAACC CATGATGATG 
GCTGCTAGGC AGCTACATGA TGAAGCCCGA AAATGGTCCA GCAAGCCTGA TGTGACTGGG 
GTTAATGAAG CCACTGAGGC TGGTGTAGAT ATAGATGAGG AGGATGATGC AGATGATGCT 
GAATTCACCC TCCCTTCTGA CATTGAAGAT GATTACGAGC CTGAGCTGCT GTTAATACCA 
AGCAATCAGC CCGTTAACCA GCCCATTCTG GCCGCTGCTC AGTCTCTACA TCGGGAAGCA 
ACCAAGTGGT CTAGTAAGGG TAATGACATC ATTGCTGCTG CCAAAAGAAT GGCTCTGCTA 
ATGGCAGAGA TGTCCCGCCT TGTGAGAGGT GGCAGTGGGA CCAAGCGTGC CCTTATTCAG 
TGTGCGAAGG ATATTGCAAA AGCATCAGAT GAAGTAACTC GACTGGCCAA GGAGGTGGCA 
AAACAGTGCA CTGACAAACG TATTCGAACC AACCTCTTGC AGGTCTGTGA GCGAATCCCA 
ACCATCAGTA CACAACTCAA AATCCTCTCT ACTGTCAAAG CTACCATGCT GGGCAGAACT 
AACATCAGTG ATGAAGAGTC AGAACAGGCT ACTGAAATGT TGGTTCATAA TGCCCAGAAC 
CTTATGCAAT CTGTAAAGGA GACCGTGAGA GAAGCAGAAG CAGCATCTAT TAAGATTAGA 
ACAGATGCTG GATTCACCCT TCGCTGGGTT AGAAAGACTC CCTGGTATCA GTAA