Bckdhb cDNA ORF clone, Chinchilla lanigera(long-tailed chinchilla)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bckdhb
Entrez Gene ID	102008389
Full Name	branched chain keto acid dehydrogenase E1 subunit beta
Gene Type	protein-coding
Organism	Chinchilla lanigera(long-tailed chinchilla)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005377677.2	XP_005377734.1	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

Pathways from BioSystems
Gene Ontology

The following Bckdhb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bckdhb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh204210	XM_005377677.2 Latest version!	Chinchilla lanigera branched chain keto acid dehydrogenase E1, beta polypeptide (Bckdhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bckdhb ( XM_005377677.2 ) cDNA ORF clone, Chinchilla lanigera(long-tailed chinchilla) -> XP_005377734.1 Chinchilla lanigera branched chain keto acid dehydrogenase E1, beta polypeptide (Bckdhb), mRNA.

Price & Availability↑

CloneID	OCh204210
Clone ID Related Accession (Same CDS sequence)	XM_005377677.2
Accession Version	XM_005377677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-18
Organism	Chinchilla lanigera(long-tailed chinchilla)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004955411.1) and transcript sequence (GBBH01117792.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 19, 2015 this sequence version replaced XM_005377677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chinchilla lanigera Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 258 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATCTTT TTCAAGCAAT AACAAGTGCC TTGGACAACT CCTTAGCCAA AGACCCTACT 
GCAGTAATAT TCGGTGAAGA TGTTGCCTTT GGCGGAGTCT TCAGATGCAC TGTTGGCTTG 
CGAGACAAAT ATGGAAAAGA TAGAGTTTTT AATACTCCAT TGTGTGAACA AGGAATTGTT 
GGATTTGGAA TCGGAATTGC AGTCACTGGT GCTACTGCCA TTGCTGAGAT TCAGTTTGCA 
GATTATATTT TCCCTGCTTT CGATCAAATC GTCAATGAAG CTGCCAAGTA TCGCTACCGC 
TCCGGGGACC TTTTTAACTG CGGAAGCCTC ACGATCAGGT CCCCGTGGGG CTGCGTTGGT 
CACGGGGCTC TCTATCATTC TCAGTGTCCC GAAGCTTTCT TCGCGCATTG CCCCGGGATC 
AAGGTGGTGA TACCCAGAAG CCCTTTCCAG GCCAAGGGCC TACTTCTGTC CTGCATAGAG 
GATAAAAATC CTTGTATATT TTTTGAACCA AAAATACTTT ACAGGGCAGC AGTGGAGCAG 
GTTCCTGTAG AACCATACAA TATTCCACTG TCCCAGGCTG AAGTCATACA GGAAGGCAGT 
GATATCACTC TGGTTGCCTG GGGCACTCAG GTTCACGTGA TCCGGGAAGT AGCTTCTATG 
GCAAGAGAAA AGCTTGGAGT GTCCTGTGAA GTCATCGACC TAAGGACTAT AATACCCTGG 
GATGTGGATA CAGTTTGCAA GTCTGTGATC AAAACGGGAC GACTCTTAAT CAGTCACGAG 
GCTCCCTTGA CAGGTGGTTT TGCATCTGAG ATCAGCTCCA CAGTTCAGGA AGAGTGTTTC 
TTGAACCTCG AAGCTCCTAT ATCCAGAGTG TGTGGTTACG ACACACCATT TCCTCACATT 
TTTGAGCCAT TTTACATCCC AGACAAATGG AAATGCTACG ATGCCCTTCG AAAAATGATC 
AACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005377734.1
CDS	364..1332
Translation	MNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRSPWGCVGHGALYHSQCPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDITLVAWGTQVHVIREVASMAREKLGVSCEVIDLRTIIPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY

Target ORF information:

RefSeq Version	XM_005377677.2
Organism	Chinchilla lanigera(long-tailed chinchilla)
Definition	Chinchilla lanigera branched chain keto acid dehydrogenase E1, beta polypeptide (Bckdhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005377677.2

ORF Insert Sequence:

ATGAATCTTT TTCAAGCAAT AACAAGTGCC TTGGACAACT CCTTAGCCAA AGACCCTACT 
GCAGTAATAT TCGGTGAAGA TGTTGCCTTT GGCGGAGTCT TCAGATGCAC TGTTGGCTTG 
CGAGACAAAT ATGGAAAAGA TAGAGTTTTT AATACTCCAT TGTGTGAACA AGGAATTGTT 
GGATTTGGAA TCGGAATTGC AGTCACTGGT GCTACTGCCA TTGCTGAGAT TCAGTTTGCA 
GATTATATTT TCCCTGCTTT CGATCAAATC GTCAATGAAG CTGCCAAGTA TCGCTACCGC 
TCCGGGGACC TTTTTAACTG CGGAAGCCTC ACGATCAGGT CCCCGTGGGG CTGCGTTGGT 
CACGGGGCTC TCTATCATTC TCAGTGTCCC GAAGCTTTCT TCGCGCATTG CCCCGGGATC 
AAGGTGGTGA TACCCAGAAG CCCTTTCCAG GCCAAGGGCC TACTTCTGTC CTGCATAGAG 
GATAAAAATC CTTGTATATT TTTTGAACCA AAAATACTTT ACAGGGCAGC AGTGGAGCAG 
GTTCCTGTAG AACCATACAA TATTCCACTG TCCCAGGCTG AAGTCATACA GGAAGGCAGT 
GATATCACTC TGGTTGCCTG GGGCACTCAG GTTCACGTGA TCCGGGAAGT AGCTTCTATG 
GCAAGAGAAA AGCTTGGAGT GTCCTGTGAA GTCATCGACC TAAGGACTAT AATACCCTGG 
GATGTGGATA CAGTTTGCAA GTCTGTGATC AAAACGGGAC GACTCTTAAT CAGTCACGAG 
GCTCCCTTGA CAGGTGGTTT TGCATCTGAG ATCAGCTCCA CAGTTCAGGA AGAGTGTTTC 
TTGAACCTCG AAGCTCCTAT ATCCAGAGTG TGTGGTTACG ACACACCATT TCCTCACATT 
TTTGAGCCAT TTTACATCCC AGACAAATGG AAATGCTACG ATGCCCTTCG AAAAATGATC 
AACTATTGA